MD07G1038400.v1.1

Protein present in Fab1, YOTB, Vac1, and EEA1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0003738
Physical Location & Seq
Reverse (-)
3156434 .. 3164189
7756 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1038400.v1.1.491

Sequence Viewer

Length: 3246 bp
ATGCTGCGTATGGACAGGATGACTGCGGATCAGGCAAAGGCGGGCCCACTCGAAAGGGACATTGACTTGGCCATCACTTCTCTGAAAAAAGGGGCACAATTGCTCAAGTATGGACGCAGAGGAAAACCCAAATTTTGTCCTTTCAGGCTTTCCAATGTCTTGAAGGATGAATCTGTTCTAATATGGTACTCAGGAAAAGAGGAGAAATATCTTAAACTAAGCCAAGTCTCGAGAATTGTACCTGGTCAGCGCACTCAAACCTTTCAGAGGTATCCTCGGCCTGAAAAGGAATATCAGTCATTTTCTCTTATATGTGATGATAGGTCTTTGGATTTGATATGCAAGGATAAAGATGAAGCTGAAGTTTGGTTTACTGGTCTAAAAGCATTAATATCACGTGGCCTTCATCAGAAGGGAAGACCAGAATCAAGAAGTGGAATTACATCTGAGGCAAACAGTCCGAGATCACACACCCAACAAAGTTCTCCATTGAGCTCTCCATTTTGTAGTGGTGATAGTTCGCAGAAGGATGGCGTGGAACCGTTTCATCTTCATGCTGCATTTGAAAGCCCTCCTAAAATTGGTTTAGAGAAGGCTTTATCGGATGTCATGTTATATGCCCTGCCTCCAAAAATCCCCTTCCCTTCAGATGCTGCTTGTGGCTCTGTTCCGTCCCCGGGAGGCTCAGATGGAATGAATGGACGAACAAAGGGTGCAGGTGTGGATGCGTTTAGAGTAAGTTTATCAAGTGCTGTTAGCTCATCAAGTCAAGGCTCTGGTCATGATGAAGTTGATGCTTTAGGGGATGTTTTCATTTGGGGGGAAGGCGTTGGTGATTGCTTTCTGGGTGGAGGATCACATAGAGTTGAAAGTTCTTCTAGTTGTAAGATGGATTCTTTTGTGCCTAAAGTCTTGGAATCTGCTGTAGTACTTGATGTTCAAAGCATAGCTTGTGGTGGACAGCATGCTGCTTTCGTAACCAAACAAGGAGAGGTATTTTCTTGGGGTGCAGAATTAGGAGGCAGACTTGGACATGGTGTGGATGCTGATGTTTCACATCCAAAACTGATAGATGCTCTGAAAAATATGAATATTGAATTTTTAGCATGTGGGGAATACCATTCTTGTGCTGTCACACTTTCCGGCGACATGTACACATGGGGTGGCAGTACTTCCAATTTTGCGCTCCTTGGTCATGGGTTTCAATCAAGTCAATGGGTCCCAAAAAAGCTAAATGGACCTTTAGAGGGAATTCATGTCTCATCAGTTTCATGTGGACCGTGGCACACAGCTGTTGTGACCTCTGCTGGGCAATTGTTTACTTTTGGGGATGGCACATTTGGTGTTCTAGGTCATGGGGACCGAAAGAGCATTTCAGTGCCCAGGGAGGTTGATTCCCTTAAAGGTCTCCGTACTGTGCGAGTAGCTTGTGGTGTTTGGCACACTGCAGCAGTAATTGAAGTTATGGTGGGGTCATCAAGTTCCAGCAATTGCTCTTCTGGAAAGCTTTTTACATGGGGAGATGGAGATAAAGGTCGACTTGGACATGGTGACAAGGAAGCAAGACTTGTGCCCACTTGTGTTGCTTCTTTGGTTGAACCCAACTTTTGTAAAGTTGCCTGTGGACAAAGCCTGACTGTTGCACTTACAACTACGGGCCATGTTTACACCATGGGAAGCCCTGTTTTTGGTCAATTAGGGGTCCCTAAAGCTGACGGGAAACTTCCAAGACGTGTTGAGGGAAAGCTAATGAAGAGTTTTGTTGAAGAAATTGCTTGTGGTGCTAATCATGTTGCAGTTTTAACTTCAAGAACTGAGGTTTACACTTGGGGAAAGGGTGCAAATGGTCGATTAGGTCTTGGGGATACAGAAGATAAATTTTCACCAACATTAGTTGAAGCTTTAAAAGATAAACAAGTCAAGAGTATTGTCTGTGGCACTAACTTTACTGCTGCTATCTGTCTTCACAAATGGCTCTCTGTTATTGATCAGTCAATGTGTTCTGGCTGTCGCCTTCCATTTAATTTTAAAAGAAAAAGACACAATTGTTATAATTGTGGACTTGTGTTATGCCATTCATGCAGCAGCAAAAAGTGTCTCAAGGCTTCCATGGCACCAAACCCAAACAAACCTTATCGTGTCTGTGATAATTGTTTTGCAAAACTTCGGAAAGCCACTGAAACCAACTCTTCATCTGGGTCTGCTATTAGTAGAAGAGGAAGTATGAATCAGGGATTCAACGAACTGATTCAAAACAATTTTATAGAAATGGAAAGTGGATCTTCCAAAAGAAACAAGAAAATGGATTTCAGCAGCAATTGTGTTATTCCCATAGCAAATGGAGTTTCTGAGCGTCGAGCACTCAATGGGACTAGATCCTTCAACCCAGTTTTTGGATCATCCAAAAAGTTCTTCTCAGCTTCGCTTCCTGGCTCCAGAATTGCATCTCGAGCAACATCACCAACATTGAGACGGTCTAGTCCACCTCGTTCCACAACACCAACCCCAGCTCTTTCGGGGCTTACTCTTCCAAAAGTTGGGGATGATGCTAAAAGGAAAAATGTCAGTCTGAGTGAAGAAGTTCTAAAATTAAAAGCTCAGGTTGAAGATCTTACTCGTAAAGCTCAACTTCGAGAAATTGAGATGGAAAAAACTGGCCAACAGTTGAAGGAGGCTCTGAAAGTAGCAGGGGAAGAAACCGTTAAATGCAAAGCAGCAAAGGAAGTAATTAAGTCGCTCACAGCCCAATTAAAGGAAATGGCTGAAAGGTTACCAATTGGAACAGCAAGAAACATGGATACTCGTCCATTAGACTCTTCCAGTCCCACTCGTGAAGTTTCTACTACAGCCGTTGAGAAAACAAGCAGTACATTGAATTTCTCAGAACCATATTCAATTGTATCGAGCAGTGAGATAATTTCTGATGTGCCACAGGCCACTTCGAATCACACTGAAGTGGCACATTCAGAAGCAACAGCTAGGCATAAAAACAGAGGAGCAAAAGCTGAAACAGCACAAGGGGATGAATGGGTTGAGCAAGATGAGCCTGGTGTATATATTACCCTAGTTTCCTTGCCTGGAGGTGTCAAAGATCTCAAGCGTGTTCGCTTCAGTCGAAAGCGGTTCAGCGAGAAACAAGCAGAGCAGTGGTGGGCGGCAAATAAGGGTAGAGTATATCAGCAGTACAATGTTCCGGTGGTTGAAAAATCCAGTATTCCGACGGGGAGAGAAGGGTTAGCTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BRX BRX_N BRX_assoc FYVE PH_12 RCC1

Protein Analysis

1082

Amino Acids

116.84

Weight (kDa)

9.09

Isoelectric Point (pI)

44.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH_12 PF16457 67 - 131 4.5e-06 Pleckstrin homology domain
WD40_RLD PF25390 269 - 652 1.5e-80 RCC1-like domain
RCC1_2 PF13540 313 - 336 2e-06 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 329 - 378 1.1e-09 Regulator of chromosome condensation (RCC1) repeat
Alsin_RLD PF25384 329 - 441 5.3e-07 Alsin RCC1-like domain
RCC1_2 PF13540 368 - 391 3.3e-06 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 381 - 433 1.8e-07 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 420 - 448 8.3e-06 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 437 - 485 1.2e-13 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 500 - 549 5.8e-14 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 553 - 601 6.8e-07 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 606 - 653 1.8e-14 Regulator of chromosome condensation (RCC1) repeat
FYVE PF01363 658 - 723 4.7e-14 FYVE zinc finger
BRX_N PF13713 898 - 928 3.6e-14 Transcription factor BRX N-terminal domain
BRX PF08381 1008 - 1062 2.1e-28 Transcription factor regulating root and shoot growth via Pin3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2052
AarI CACCTGC 1 cut(s) 707
AasI GACNNNNNNGTC 1 cut(s) 2478
Acc36I ACCTGC 1 cut(s) 707
AccB1I GGYRCC 1 cut(s) 2113
AccB7I CCANNNNNTGG 2 cut(s) 2393, 2537
AccI GTMKAC 1 cut(s) 1537
AciI CCGC 4 cut(s) 26, 41, 3121, 3155
AclWI GGATC 5 cut(s) 36, 862, 2287, 2370, 2404
AcoI YGGCCR 2 cut(s) 69, 2656
AcsI RAATTY 5 cut(s) 131, 1097, 1251, 1877, 2875
AcuI CTGAAG 4 cut(s) 381, 630, 2973, 3094
AcvI CACGTG 1 cut(s) 398
AfaI GTAC 8 cut(s) 188, 240, 930, 1154, 1171, 1414, 2869, 3185
AfiI CCNNNNNNNGG 9 cut(s) 267, 581, 677, 1247, 1308, 1688, 2393, 2537, 2752
AflIII ACRYGT 2 cut(s) 1149, 1732
AjiI CACGTC 1 cut(s) 1733
AjnI CCWGG 5 cut(s) 241, 1382, 2428, 3046, 3076
AjuI GAANNNNNNNTTGG 2 cut(s) 2396, 2428
AleI CACNNNNGTG 1 cut(s) 2954
Alw21I GWGCWC 2 cut(s) 497, 2362
Alw26I GTCTC 5 cut(s) 232, 1264, 1412, 2102, 2464
AlwI GGATC 5 cut(s) 36, 862, 2287, 2370, 2404
AlwNI CAGNNNCTG 1 cut(s) 653
Ama87I CYCGRG 3 cut(s) 229, 676, 2448
AoxI GGCC 7 cut(s) 43, 69, 278, 400, 1657, 2656, 2934
ApaI GGGCCC 1 cut(s) 47
ApoI RAATTY 5 cut(s) 131, 1097, 1251, 1877, 2875
AseI ATTAAT 1 cut(s) 389
Asp700I GAANNNNTTC 4 cut(s) 174, 543, 2247, 2580
AspLEI GCGC 2 cut(s) 252, 1186
AspS9I GGNCC 8 cut(s) 43, 44, 1219, 1238, 1277, 1360, 1657, 1702
AsuC2I CCSGG 2 cut(s) 677, 678
AsuHPI GGTGA 5 cut(s) 524, 845, 1562, 1875, 2451
AsuII TTCGAA 1 cut(s) 2942
AvaI CYCGRG 3 cut(s) 229, 676, 2448
AvaII GGWCC 5 cut(s) 1219, 1238, 1277, 1360, 1702
BaeGI GKGCMC 4 cut(s) 47, 97, 1383, 1575
BalI TGGCCA 2 cut(s) 71, 2658
BanI GGYRCC 1 cut(s) 2113
BanII GRGCYC 2 cut(s) 47, 497
BarI GAAGNNNNNNTAC 2 cut(s) 424, 456
BauI CACGAG 1 cut(s) 2829
BbrPI CACGTG 1 cut(s) 398
BbsI GAAGAC 2 cut(s) 424, 1955
Bbv12I GWGCWC 2 cut(s) 497, 2362
BbvI GCAGC 9 cut(s) 544, 640, 955, 1460, 1939, 2094, 2097, 2325, 2726
BccI CCATC 7 cut(s) 80, 524, 683, 883, 1325, 1517, 2638
BceAI ACGGC 1 cut(s) 2834
BciT130I CCWGG 5 cut(s) 243, 1384, 2430, 3048, 3078
BciVI GTATCC 3 cut(s) 282, 1858, 2791
BclI TGATCA 1 cut(s) 1987
BcnI CCSGG 2 cut(s) 677, 678
BcoDI GTCTC 5 cut(s) 232, 1264, 1412, 2102, 2464
BfaI CTAG 6 cut(s) 879, 1349, 2373, 2478, 2979, 3065
BfmI CTRYAG 3 cut(s) 924, 1446, 2844
BfuAI ACCTGC 1 cut(s) 707
BfuI GTATCC 3 cut(s) 282, 1858, 2791
BglII AGATCT 2 cut(s) 2608, 3091
BmcAI AGTACT 2 cut(s) 930, 1171
Bme1390I CCNGG 7 cut(s) 243, 677, 678, 1384, 2430, 3048, 3078
Bme18I GGWCC 5 cut(s) 1219, 1238, 1277, 1360, 1702
BmeT110I CYCGRG 3 cut(s) 229, 676, 2448
BmgBI CACGTC 1 cut(s) 1733
BmgT120I GGNCC 8 cut(s) 43, 44, 1219, 1238, 1277, 1360, 1657, 1702
BmiI GGNNCC 9 cut(s) 45, 540, 1220, 1221, 1361, 1703, 1704, 2115, 2434
BmrFI CCNGG 7 cut(s) 243, 677, 678, 1384, 2430, 3048, 3078
BmrI ACTGGG 1 cut(s) 2381
BmsI GCATC 7 cut(s) 640, 715, 784, 1033, 1063, 2453, 2536
BmuI ACTGGG 1 cut(s) 2381
BpiI GAAGAC 2 cut(s) 424, 1955
BplI GAGNNNNNCTC 2 cut(s) 259, 291
BpmI CTGGAG 2 cut(s) 2419, 3099
Bpu10I CCTNAGC 1 cut(s) 2598
Bpu14I TTCGAA 1 cut(s) 2942
BpuEI CTTGAG 3 cut(s) 89, 2084, 3080
BpuMI CCSGG 2 cut(s) 677, 678
BsaAI YACGTR 1 cut(s) 398
BsaI GGTCTC 1 cut(s) 1412
BsaJI CCNNGG 9 cut(s) 275, 675, 676, 1189, 1280, 1382, 1383, 1671, 2109
BsaWI WCCGGW 1 cut(s) 3193
Bsc4I CCNNNNNNNGG 9 cut(s) 267, 581, 677, 1247, 1308, 1688, 2393, 2537, 2752
Bse1I ACTGG 5 cut(s) 379, 2387, 2659, 2820, 3210
BseBI CCWGG 5 cut(s) 243, 1384, 2430, 3048, 3078
BseDI CCNNGG 9 cut(s) 275, 675, 676, 1189, 1280, 1382, 1383, 1671, 2109
BseLI CCNNNNNNNGG 9 cut(s) 267, 581, 677, 1247, 1308, 1688, 2393, 2537, 2752
BseMII CTCAG 9 cut(s) 204, 438, 699, 1806, 2340, 2430, 2561, 2612, 2895
BseNI ACTGG 5 cut(s) 379, 2387, 2659, 2820, 3210
BseRI GAGGAG 2 cut(s) 215, 3009
BseSI GKGCMC 4 cut(s) 47, 97, 1383, 1575
BseXI GCAGC 9 cut(s) 544, 640, 955, 1460, 1939, 2094, 2097, 2325, 2726
BseYI CCCAGC 2 cut(s) 1307, 2506
BsgI GTGCAG 2 cut(s) 735, 1029
BshFI GGCC 7 cut(s) 45, 71, 280, 402, 1659, 2658, 2936
BshNI GGYRCC 1 cut(s) 2113
BsiHKAI GWGCWC 2 cut(s) 497, 2362
BsiHKCI CYCGRG 3 cut(s) 229, 676, 2448
BsiSI CCGG 3 cut(s) 677, 1143, 3194
BslFI GGGAC 7 cut(s) 71, 658, 1205, 1373, 1688, 2383, 2808
BslI CCNNNNNNNGG 9 cut(s) 267, 581, 677, 1247, 1308, 1688, 2393, 2537, 2752
BsmAI GTCTC 5 cut(s) 232, 1264, 1412, 2102, 2464
BsmBI CGTCTC 1 cut(s) 2464
BsmFI GGGAC 7 cut(s) 71, 658, 1205, 1373, 1688, 2383, 2808
BsnI GGCC 7 cut(s) 45, 71, 280, 402, 1659, 2658, 2936
Bso31I GGTCTC 1 cut(s) 1412
BsoBI CYCGRG 3 cut(s) 229, 676, 2448
Bsp119I TTCGAA 1 cut(s) 2942
Bsp120I GGGCCC 1 cut(s) 43
Bsp1286I GDGCHC 6 cut(s) 47, 97, 497, 1383, 1575, 2362
Bsp1407I TGTACA 1 cut(s) 1152
Bsp143I GATC 9 cut(s) 28, 464, 854, 1987, 2279, 2375, 2396, 2608, 3091
Bsp19I CCATGG 2 cut(s) 1671, 2109
BspACI CCGC 4 cut(s) 26, 41, 3121, 3155
BspANI GGCC 7 cut(s) 45, 71, 280, 402, 1659, 2658, 2936
BspCNI CTCAG 9 cut(s) 203, 439, 698, 1807, 2341, 2429, 2562, 2611, 2894
BspHI TCATGA 1 cut(s) 781
BspLI GGNNCC 9 cut(s) 45, 540, 1220, 1221, 1361, 1703, 1704, 2115, 2434
BspMAI CTGCAG 1 cut(s) 1450
BspMI ACCTGC 1 cut(s) 707
BspPI GGATC 5 cut(s) 36, 862, 2287, 2370, 2404
BspQI GCTCTTC 1 cut(s) 1501
BspT104I TTCGAA 1 cut(s) 2942
BspT107I GGYRCC 1 cut(s) 2113
BspTNI GGTCTC 1 cut(s) 1412
BsrGI TGTACA 1 cut(s) 1152
BsrI ACTGG 5 cut(s) 379, 2387, 2659, 2820, 3210
BssECI CCNNGG 9 cut(s) 275, 675, 676, 1189, 1280, 1382, 1383, 1671, 2109
BssMI GATC 9 cut(s) 28, 464, 854, 1987, 2279, 2375, 2396, 2608, 3091
BssSI CACGAG 1 cut(s) 2829
BssT1I CCWWGG 3 cut(s) 1189, 1671, 2109
Bst2BI CACGAG 1 cut(s) 2829
Bst2UI CCWGG 5 cut(s) 243, 1384, 2430, 3048, 3078
Bst4CI ACNGT 8 cut(s) 458, 543, 1281, 1417, 1639, 2475, 2664, 2701
Bst6I CTCTTC 6 cut(s) 1501, 1748, 2194, 2209, 2532, 2821
BstAUI TGTACA 1 cut(s) 1152
BstBAI YACGTR 1 cut(s) 398
BstBI TTCGAA 1 cut(s) 2942
BstC8I GCNNGC 2 cut(s) 43, 966
BstDSI CCRYGG 3 cut(s) 1280, 1671, 2109
BstEII GGTNACC 1 cut(s) 2769
BstENI CCTNNNNNAGG 1 cut(s) 265
BstHHI GCGC 2 cut(s) 252, 1186
BstKTI GATC 9 cut(s) 31, 467, 857, 1990, 2282, 2378, 2399, 2611, 3094
BstMAI GTCTC 5 cut(s) 232, 1264, 1412, 2102, 2464
BstMBI GATC 9 cut(s) 28, 464, 854, 1987, 2279, 2375, 2396, 2608, 3091
BstMWI GCNNNNNNNGC 7 cut(s) 32, 1781, 2079, 2111, 2450, 3011, 3043
BstNI CCWGG 5 cut(s) 243, 1384, 2430, 3048, 3078
BstNSI RCATGY 3 cut(s) 968, 1110, 1153
BstPI GGTNACC 1 cut(s) 2769
BstSCI CCNGG 7 cut(s) 241, 675, 676, 1382, 2428, 3046, 3076
BstSFI CTRYAG 3 cut(s) 924, 1446, 2844
BstSLI GKGCMC 4 cut(s) 47, 97, 1383, 1575
BstV1I GCAGC 9 cut(s) 544, 640, 955, 1460, 1939, 2094, 2097, 2325, 2726
BstV2I GAAGAC 2 cut(s) 424, 1955
BstX2I RGATCY 4 cut(s) 2279, 2375, 2608, 3091
BstYI RGATCY 4 cut(s) 2279, 2375, 2608, 3091
BsuI GTATCC 3 cut(s) 282, 1858, 2791
BsuRI GGCC 7 cut(s) 45, 71, 280, 402, 1659, 2658, 2936
BtgI CCRYGG 3 cut(s) 1280, 1671, 2109
BtrI CACGTC 1 cut(s) 1733
BtsI GCAGTG 3 cut(s) 1443, 2914, 3152
BtsIMutI CAGTG 6 cut(s) 1383, 1443, 2175, 2914, 2949, 3152
BveI ACCTGC 1 cut(s) 707
Cac8I GCNNGC 2 cut(s) 43, 966
CaiI CAGNNNCTG 1 cut(s) 653
CciI TCATGA 1 cut(s) 781
CfoI GCGC 2 cut(s) 252, 1186
Cfr13I GGNCC 8 cut(s) 43, 44, 1219, 1238, 1277, 1360, 1657, 1702
Cfr9I CCCGGG 1 cut(s) 676
CseI GACGC 2 cut(s) 123, 2342
CsiI ACCWGGT 1 cut(s) 241
Csp6I GTAC 8 cut(s) 187, 239, 929, 1153, 1170, 1413, 2868, 3184
CspCI CAANNNNNGTGG 2 cut(s) 1564, 1599
CviQI GTAC 8 cut(s) 187, 239, 929, 1153, 1170, 1413, 2868, 3184
DpnI GATC 9 cut(s) 30, 466, 856, 1989, 2281, 2377, 2398, 2610, 3093
DpnII GATC 9 cut(s) 28, 464, 854, 1987, 2279, 2375, 2396, 2608, 3091
DraI TTTAAA 2 cut(s) 1905, 2029
DrdI GACNNNNNNGTC 1 cut(s) 2478
DseDI GACNNNNNNGTC 1 cut(s) 2478
EaeI YGGCCR 2 cut(s) 69, 2656
Eam1104I CTCTTC 6 cut(s) 1501, 1748, 2194, 2209, 2532, 2821
EarI CTCTTC 6 cut(s) 1501, 1748, 2194, 2209, 2532, 2821
Ecl136II GAGCTC 1 cut(s) 495
Eco130I CCWWGG 3 cut(s) 1189, 1671, 2109
Eco24I GRGCYC 2 cut(s) 47, 497
Eco31I GGTCTC 1 cut(s) 1412
Eco47I GGWCC 5 cut(s) 1219, 1238, 1277, 1360, 1702
Eco53kI GAGCTC 1 cut(s) 495
Eco57I CTGAAG 4 cut(s) 381, 630, 2973, 3094
Eco72I CACGTG 1 cut(s) 398
Eco88I CYCGRG 3 cut(s) 229, 676, 2448
Eco91I GGTNACC 1 cut(s) 2769
EcoICRI GAGCTC 1 cut(s) 495
EcoNI CCTNNNNNAGG 1 cut(s) 265
EcoO109I RGGNCCY 2 cut(s) 1219, 1702
EcoO65I GGTNACC 1 cut(s) 2769
EcoRI GAATTC 1 cut(s) 1251
EcoRII CCWGG 5 cut(s) 241, 1382, 2428, 3046, 3076
EcoT14I CCWWGG 3 cut(s) 1189, 1671, 2109
EcoT38I GRGCYC 2 cut(s) 47, 497
ErhI CCWWGG 3 cut(s) 1189, 1671, 2109
Esp3I CGTCTC 1 cut(s) 2464
FaqI GGGAC 7 cut(s) 71, 658, 1205, 1373, 1688, 2383, 2808
FauI CCCGC 1 cut(s) 34
FbaI TGATCA 1 cut(s) 1987
FblI GTMKAC 1 cut(s) 1537
FriOI GRGCYC 2 cut(s) 47, 497
FspBI CTAG 6 cut(s) 879, 1349, 2373, 2478, 2979, 3065
GlaI GCGC 2 cut(s) 251, 1185
GsaI CCCAGC 2 cut(s) 1311, 2510
GsuI CTGGAG 2 cut(s) 2419, 3099
HaeIII GGCC 7 cut(s) 45, 71, 280, 402, 1659, 2658, 2936
HapII CCGG 3 cut(s) 677, 1143, 3194
HgaI GACGC 2 cut(s) 123, 2342
HhaI GCGC 2 cut(s) 252, 1186
Hin6I GCGC 2 cut(s) 250, 1184
HinP1I GCGC 2 cut(s) 250, 1184
HincII GTYRAC 1 cut(s) 1538
HindII GTYRAC 1 cut(s) 1538
HindIII AAGCTT 2 cut(s) 1505, 1899
HpaII CCGG 3 cut(s) 677, 1143, 3194
HphI GGTGA 5 cut(s) 524, 845, 1562, 1875, 2451
Hpy99I CGWCG 2 cut(s) 2358, 3223
HpyCH4III ACNGT 8 cut(s) 458, 543, 1281, 1417, 1639, 2475, 2664, 2701
HpyCH4IV ACGT 2 cut(s) 397, 1732
HpyF10VI GCNNNNNNNGC 7 cut(s) 32, 1781, 2079, 2111, 2450, 3011, 3043
HpySE526I ACGT 2 cut(s) 397, 1732
HspAI GCGC 2 cut(s) 250, 1184
KflI GGGWCCC 2 cut(s) 1219, 1702
Ksp22I TGATCA 1 cut(s) 1987
Kzo9I GATC 9 cut(s) 28, 464, 854, 1987, 2279, 2375, 2396, 2608, 3091
LguI GCTCTTC 1 cut(s) 1501
LmnI GCTCC 3 cut(s) 1191, 2438, 2996
Lsp1109I GCAGC 9 cut(s) 544, 640, 955, 1460, 1939, 2094, 2097, 2325, 2726
LweI GCATC 7 cut(s) 640, 715, 784, 1033, 1063, 2453, 2536
MabI ACCWGGT 1 cut(s) 241
MaeI CTAG 6 cut(s) 879, 1349, 2373, 2478, 2979, 3065
MaeII ACGT 2 cut(s) 397, 1732
MaeIII GTNAC 5 cut(s) 976, 1132, 1297, 1550, 2769
MalI GATC 9 cut(s) 30, 466, 856, 1989, 2281, 2377, 2398, 2610, 3093
MboI GATC 9 cut(s) 28, 464, 854, 1987, 2279, 2375, 2396, 2608, 3091
MfeI CAATTG 7 cut(s) 98, 1313, 1489, 2044, 2317, 2775, 2895
MflI RGATCY 4 cut(s) 2279, 2375, 2608, 3091
MhlI GDGCHC 6 cut(s) 47, 97, 497, 1383, 1575, 2362
MlsI TGGCCA 2 cut(s) 71, 2658
MluNI TGGCCA 2 cut(s) 71, 2658
MlyI GAGTC 1 cut(s) 2807
MmeI TCCRAC 1 cut(s) 3242
Mox20I TGGCCA 2 cut(s) 71, 2658
MroXI GAANNNNTTC 4 cut(s) 174, 543, 2247, 2580
MscI TGGCCA 2 cut(s) 71, 2658
MslI CAYNNNNRTG 4 cut(s) 552, 1125, 1376, 2954
Msp20I TGGCCA 2 cut(s) 71, 2658
MspA1I CMGCKG 1 cut(s) 1292
MspI CCGG 3 cut(s) 677, 1143, 3194
MspR9I CCNGG 7 cut(s) 243, 677, 678, 1384, 2430, 3048, 3078
MunI CAATTG 7 cut(s) 98, 1313, 1489, 2044, 2317, 2775, 2895
MvaI CCWGG 5 cut(s) 243, 1384, 2430, 3048, 3078
MwoI GCNNNNNNNGC 7 cut(s) 32, 1781, 2079, 2111, 2450, 3011, 3043
NciI CCSGG 2 cut(s) 677, 678
NcoI CCATGG 2 cut(s) 1671, 2109
NdeII GATC 9 cut(s) 28, 464, 854, 1987, 2279, 2375, 2396, 2608, 3091
NlaIV GGNNCC 9 cut(s) 45, 540, 1220, 1221, 1361, 1703, 1704, 2115, 2434
NmeAIII GCCGAG 1 cut(s) 256
NmuCI GTSAC 3 cut(s) 1132, 1297, 1550
NspI RCATGY 3 cut(s) 968, 1110, 1153
NspV TTCGAA 1 cut(s) 2942
OliI CACNNNNGTG 1 cut(s) 2954
PaeI GCATGC 1 cut(s) 968
PaeR7I CTCGAG 2 cut(s) 229, 2448
PagI TCATGA 1 cut(s) 781
PaqCI CACCTGC 1 cut(s) 707
PasI CCCWGGG 1 cut(s) 1383
PciI ACATGT 1 cut(s) 1149
PciSI GCTCTTC 1 cut(s) 1501
PcsI WCGNNNNNNNCGW 1 cut(s) 3112
PdmI GAANNNNTTC 4 cut(s) 174, 543, 2247, 2580
PfeI GAWTC 9 cut(s) 170, 425, 893, 917, 1394, 2227, 2235, 2248, 2944
PflMI CCANNNNNTGG 2 cut(s) 2393, 2537
PleI GAGTC 1 cut(s) 2807
PmaCI CACGTG 1 cut(s) 398
PmlI CACGTG 1 cut(s) 398
PpsI GAGTC 1 cut(s) 2807
Ppu21I YACGTR 1 cut(s) 398
PpuMI RGGWCCY 2 cut(s) 1219, 1702
PscI ACATGT 1 cut(s) 1149
PshBI ATTAAT 1 cut(s) 389
PsiI TTATAA 1 cut(s) 2052
Psp124BI GAGCTC 1 cut(s) 497
Psp5II RGGWCCY 2 cut(s) 1219, 1702
Psp6I CCWGG 5 cut(s) 241, 1382, 2428, 3046, 3076
PspCI CACGTG 1 cut(s) 398
PspEI GGTNACC 1 cut(s) 2769
PspFI CCCAGC 2 cut(s) 1307, 2506
PspGI CCWGG 5 cut(s) 241, 1382, 2428, 3046, 3076
PspN4I GGNNCC 9 cut(s) 45, 540, 1220, 1221, 1361, 1703, 1704, 2115, 2434
PspOMI GGGCCC 1 cut(s) 43
PspPI GGNCC 8 cut(s) 43, 44, 1219, 1238, 1277, 1360, 1657, 1702
PspPPI RGGWCCY 2 cut(s) 1219, 1702
PsrI GAACNNNNNNTAC 2 cut(s) 921, 953
PstI CTGCAG 1 cut(s) 1450
PstNI CAGNNNCTG 1 cut(s) 653
PsuI RGATCY 4 cut(s) 2279, 2375, 2608, 3091
PvuII CAGCTG 1 cut(s) 1292
RsaI GTAC 8 cut(s) 188, 240, 930, 1154, 1171, 1414, 2869, 3185
RsaNI GTAC 8 cut(s) 187, 239, 929, 1153, 1170, 1413, 2868, 3184
RseI CAYNNNNRTG 4 cut(s) 552, 1125, 1376, 2954
SacI GAGCTC 1 cut(s) 497
SalI GTCGAC 1 cut(s) 1536
SapI GCTCTTC 1 cut(s) 1501
Sau3AI GATC 9 cut(s) 28, 464, 854, 1987, 2279, 2375, 2396, 2608, 3091
Sau96I GGNCC 8 cut(s) 43, 44, 1219, 1238, 1277, 1360, 1657, 1702
ScaI AGTACT 2 cut(s) 930, 1171
SchI GAGTC 1 cut(s) 2807
ScrFI CCNGG 7 cut(s) 243, 677, 678, 1384, 2430, 3048, 3078
SduI GDGCHC 6 cut(s) 47, 97, 497, 1383, 1575, 2362
SexAI ACCWGGT 1 cut(s) 241
SfaNI GCATC 7 cut(s) 640, 715, 784, 1033, 1063, 2453, 2536
SfcI CTRYAG 3 cut(s) 924, 1446, 2844
Sfr274I CTCGAG 2 cut(s) 229, 2448
SfuI TTCGAA 1 cut(s) 2942
SinI GGWCC 5 cut(s) 1219, 1238, 1277, 1360, 1702
SlaI CTCGAG 2 cut(s) 229, 2448
SmaI CCCGGG 1 cut(s) 678
SmiMI CAYNNNNRTG 4 cut(s) 552, 1125, 1376, 2954
SmlI CTYRAG 5 cut(s) 104, 229, 2099, 2448, 3095
SmoI CTYRAG 5 cut(s) 104, 229, 2099, 2448, 3095
SphI GCATGC 1 cut(s) 968
SsiI CCGC 4 cut(s) 26, 41, 3121, 3155
SspI AATATT 1 cut(s) 1093
SspMI CTAG 6 cut(s) 879, 1349, 2373, 2478, 2979, 3065
SstI GAGCTC 1 cut(s) 497
StyD4I CCNGG 7 cut(s) 241, 675, 676, 1382, 2428, 3046, 3076
StyI CCWWGG 3 cut(s) 1189, 1671, 2109
TaaI ACNGT 8 cut(s) 458, 543, 1281, 1417, 1639, 2475, 2664, 2701
TaiI ACGT 2 cut(s) 400, 1735
TaqII GACCGA 1 cut(s) 1377
TatI WGTACW 5 cut(s) 928, 1152, 1169, 2867, 3183
TauI GCSGC 1 cut(s) 3158
TfiI GAWTC 9 cut(s) 170, 425, 893, 917, 1394, 2227, 2235, 2248, 2944
TscAI CASTG 6 cut(s) 1383, 1450, 2182, 2914, 2956, 3152
TseFI GTSAC 3 cut(s) 1132, 1297, 1550
Tsp45I GTSAC 3 cut(s) 1132, 1297, 1550
TspGWI ACGGA 2 cut(s) 660, 1400
TspMI CCCGGG 1 cut(s) 676
TspRI CASTG 6 cut(s) 1383, 1450, 2182, 2914, 2956, 3152
Van91I CCANNNNNTGG 2 cut(s) 2393, 2537
VpaK11BI GGWCC 5 cut(s) 1219, 1238, 1277, 1360, 1702
VspI ATTAAT 1 cut(s) 389
XagI CCTNNNNNAGG 1 cut(s) 265
XapI RAATTY 5 cut(s) 131, 1097, 1251, 1877, 2875
XceI RCATGY 3 cut(s) 968, 1110, 1153
XhoI CTCGAG 2 cut(s) 229, 2448
XmaI CCCGGG 1 cut(s) 676
XmiI GTMKAC 1 cut(s) 1537
XmnI GAANNNNTTC 4 cut(s) 174, 543, 2247, 2580
XspI CTAG 6 cut(s) 879, 1349, 2373, 2478, 2979, 3065
ZrmI AGTACT 2 cut(s) 930, 1171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.