Rw0G011380

Protein present in Fab1, YOTB, Vac1, and EEA1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00500
OG0003738
Physical Location & Seq
Forward (+)
16013 .. 27609
11597 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G011380.1

Sequence Viewer

Length: 3216 bp
ATGTTGCGCATGGAGAGGATGACTGCCGATCAGGCGAAGGCGGGCCCACTCGAAAGGGACATTCATTTGGCCATCACTGCTCTAAAAAAGGGGTCACAATTACTGAAGTATGGACGCAGAGGAAAGCCCAAATTTTGTCCTTTCAGACTGTCCAATGATGAATCTGTTCTGACATGGTACTCAGGCAAAGATGAGAAATATGTTAAACTAAGCCATGTCTCTAGAATTATACCTGGCCAACGCACTCCAATCTTTCAAAGGTATCCTCGGCCTGAGAAGGAATATCAGTCATTTTCTCTTATATATGATGACAGGTCTTTAGATTTGATATGTAAGGATAAAGATGAAGCTGAAGTCTGGTTTACTGGTCTAAAAGCATTAATATCACGTGGCCTTCAACAGAAGGGAAGAGCAGAAACGAAAAGTGGAGTTACCTCTGAAGCAACTAGTCCAAGATCACACACGCAAAGAAGTTCTCCTTTGAGCTCTCCATTTTGTAGTGGTGATAGTTCACAGAAGGATGAAGTGGAACCCTTTCATCCTCATGCTACAGCTGAAAGCCCTCCTAAAGTCCATTTGGAGAAGGCTTTATCAGATGCCATGTTATATGCCCTCCCTCCGAAAGTTCCCTTTCCTTCAGATCCTGCTTCTGATGGAATGAATGGTCGAACAAAGGGTGCAGGGACGGATGCTTTTAGAGTTAGTTTATCAAGTGCAGTTAGCTCATCAAGTCAAGGCTCTGGTCATGATGATGGTGATGCTTTAGGGGATGTTTTCATTTGGGGGGAAGGCGTAGGTGATTGCATTTTGGGTGGTGGATTACATAGAGTTGGTAGTTCTTCTAGTTCTAAGACGGACTCTTTTGTACCCAAAGTCTTGGAATCTGCTGTTGTACTTGATGTTCAAAGTATAGCTTGTGGGGGAAGACATGCTGCTTTTGTAACCAAGCAAGGGGAGGTATTTTCTTGGGGTGAAGAATTAGGAGGCAGACTCGGACATGGTGTAGATGCTGATATCTCCCAACCAAAGCTCATAGAAGGTCTCAAAAATCTTAACGTAGAATTTTTAGCCTGTGGGGAGTACCATTCTTGTGCAGTAACACTCTCTGGTGACATATACACATGGGGTGGCAGTACTTCTAATTTTGCCCTCCTTGGGCATGGGTTTCAATCGAGTCAGTGGGTCCCAAAAAAGTTAAATGGACCTTTAGAGGGAATTCATGTCTCTTCAGTTTCTTGTGGACCATGGCACACAGCCGTTGTGACCTCTGCTGGTCAATTGTTTACTTTTGGTGATGGAACTTTTGGTGTTCTAGGTCATGGGGATCGCAAGAGTATATCAGTTCCAAGGGAAGTTGAGTCCCTTAAAGGCCTTCGTACTGTTCGAGTAGCTTGTGGTGTTTGGCACTCTGCAGCAGTAATTGAAGTAATGGTGGGATCATCAAGTTCCAGCAACTGTTCTTCTGGGAAGCTTTTTACATGGGGAGATGGAGATAAAGGTCGACTTGGGCATGGTGATAAGGATGCAAGACTTGTGCCCACTTGCGTTGCTGCTTTGGTTGACCCGAACTTCTGTCAAGTTGCGTGTGGACAAAGTCTGACTGTTGCGCTTACAACAACTGGGCATGTTTACACAATGGGAAGCCCTGTTTTTGGTCAATTAGGTATCCCTCAAGCTGACGGGAAACTCCCCCGCCGTGTTGAGGGAAAACTAATGAAGAATTTTGTTGAAGAAATTGCTTGTGGTGCTTATCATGTTGCAGTCTTAACTTCGAGAACTGAAGTTTACACTTGGGGAAAGGGTGCAAATGGTAGATTAGGTCTTGGGGATACAGAGGATAAATTTTCACCAACATTAGTTGAAGCTCTAAGAGACAAACAAGTCAAAAGTATCGTTTGTGGCACTAACTTTACAGCAGCCATCTGTCTTCATAAATGGGTCTCTGGTGTCGATCAATCAATGTGTTCTGGCTGTCGCCTTCCGTTTAATTTTAAAAGAAAAAGACACAACTGCTACAATTGTGGACTTGTATTATGCCATTCATGCAGTAGCAAAAAGTGCCTCAAGGCTTCGATGGCACCAAATCCAAACAAACCATATCGAGTTTGTGATAATTGTTTTGGGAAACTCAGAAAGGCCTCTGAAACCAACTCTTCATCTGGCTCCGCTTTGAAAAAAGGAAGTAATCAGGGATTTGCTGAACTGAGTGAAAAAAATATTAAAGATTTGGACACTGGTTCATCCACAAGAAACAAGAAACTAGATTTTAGCAGTAATTGTGTTGCTCCCTTTCCAAATGGCATTTCTGAGCGCCGTGCATTCAGTGGGACTAAATCTTTCAACCCAGTATTTGGATCTTCCAAAAAGTTTTTCTCAGCTTCTCTTCCTGGATCAAGAATTGCTTCTCGAGCAACATCACCAACATCGAGACGATCTAGTCCACCTCGTGCCAAAACGCCAACCCCTGCTCTCTCAGGGATCACATTGCCAAATGTTGTGGAATATGCTAGAGGGACAAATGACGGTCTAAGTGAAGAAATTATTAAACTAAGAGCTCAGGTCGAAGATCTTACTCGTAAAAGTCGAGTTCAAGATACTGAGCTGGAAAGAACTACCCACCAATTGAAGGAGGCTGTCAAAGTAGCAGACGAAGAAACTGCAAAATGTAAAGCAGCAAAGGAAGTAATTAAGTCGCTCACAGCTCAATTAAAGGAAATGGCTGAAAGGTTACCCATTGGGACAGCAAGAAGCAGCGACACACGTCCATTAGGCTTTTCTAGTCCTACCCATGACGTTTCTACTACAGCCATTGAGAAAGCAAGCAGTCCATTGACCTTCCATGAACCACATTCAATTGCATCAAATAGCCTGGTGGTTTCTAATGCATCAAGGATTTTGAGTAATCATGCTTCAAATCCCACTGAAGTGGCAAATTCGGAAGCAACTACTAGGCATAAGAACAGAGGAACAAAAGCTGAAACCACAAATGGGGATGAATGGGTTGAGCAAGATGAGCCTGGTGTATATATTACCCTGGTTTCCTTGCCCACAGGAGTCAAAGATCTCAAGCGTGTCCGCTTCAGTCGAAAGCGGTTCAGTGAGAAACAAGCGGAACAGTGGTGGGCGGCAAATAGGACTAGAGTATACCAGCAGTACAATGTTCCTGTGGTTGAAAAATCTAGTATTCCCATAGGAAGAGAAGGGTTAGCTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BRX BRX_N BRX_assoc FYVE PH_12 RCC1

Protein Analysis

1071

Amino Acids

115.75

Weight (kDa)

9.11

Isoelectric Point (pI)

40.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH_12 PF16457 26 - 128 1e-07 Pleckstrin homology domain
WD40_RLD PF25390 257 - 640 2.7e-81 RCC1-like domain
RCC1_2 PF13540 301 - 324 2.5e-06 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 317 - 366 6.7e-10 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 356 - 379 4.1e-06 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 369 - 421 9.7e-08 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 408 - 436 8.2e-06 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 425 - 473 4.4e-14 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 488 - 537 6.2e-14 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 541 - 589 3.1e-07 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 594 - 641 1.5e-14 Regulator of chromosome condensation (RCC1) repeat
FYVE PF01363 645 - 711 2.2e-14 FYVE zinc finger
BRX_N PF13713 884 - 917 1.2e-14 Transcription factor BRX N-terminal domain
BRX_assoc PF16627 924 - 988 1.1e-10 Unstructured region between BRX_N and BRX domain
BRX PF08381 998 - 1052 1.9e-28 Transcription factor regulating root and shoot growth via Pin3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1880, 2436
Acc16I TGCGCA 1 cut(s) 8
AccB1I GGYRCC 1 cut(s) 2077
AccB7I CCANNNNNTGG 1 cut(s) 2351
AccI GTMKAC 2 cut(s) 1501, 3144
AciI CCGC 7 cut(s) 41, 1693, 2166, 3076, 3091, 3110, 3125
AclWI GGATC 6 cut(s) 635, 1332, 1444, 2362, 2398, 2486
AcoI YGGCCR 2 cut(s) 69, 235
AcsI RAATTY 6 cut(s) 131, 1061, 1215, 1720, 1841, 2932
AcuI CTGAAG 8 cut(s) 125, 372, 459, 621, 1212, 1800, 2943, 3064
AcvI CACGTG 1 cut(s) 389
AdeI CACNNNGTG 1 cut(s) 2447
AfaI GTAC 7 cut(s) 179, 867, 894, 1082, 1135, 1378, 3155
AfiI CCNNNNNNNGG 8 cut(s) 951, 1155, 1211, 1652, 1702, 2351, 2626, 2988
AflIII ACRYGT 1 cut(s) 2759
AhlI ACTAGT 1 cut(s) 446
AjiI CACGTC 1 cut(s) 2762
AjnI CCWGG 5 cut(s) 232, 2386, 2868, 3016, 3033
AjuI GAANNNNNNNTTGG 4 cut(s) 2287, 2319, 2354, 2386
AleI CACNNNNGTG 1 cut(s) 2924
Alw21I GWGCWC 2 cut(s) 488, 2557
Alw26I GTCTC 6 cut(s) 223, 1046, 1228, 1866, 1945, 2422
AlwI GGATC 6 cut(s) 635, 1332, 1444, 2362, 2398, 2486
AlwNI CAGNNNCTG 2 cut(s) 644, 1455
Ama87I CYCGRG 1 cut(s) 2406
AoxI GGCC 7 cut(s) 43, 69, 235, 269, 391, 1369, 2136
ApaI GGGCCC 1 cut(s) 47
ApeKI GCWGC 6 cut(s) 932, 1412, 1550, 1916, 2672, 2751
ApoI RAATTY 6 cut(s) 131, 1061, 1215, 1720, 1841, 2932
AseI ATTAAT 1 cut(s) 380
Asp700I GAANNNNTTC 3 cut(s) 165, 534, 2401
AspLEI GCGC 3 cut(s) 9, 1609, 2313
AspS9I GGNCC 5 cut(s) 43, 44, 1183, 1202, 1241
AsuHPI GGTGA 9 cut(s) 515, 767, 809, 983, 1121, 1304, 1526, 1839, 2409
AvaI CYCGRG 1 cut(s) 2406
AvaII GGWCC 3 cut(s) 1183, 1202, 1241
BaeGI GKGCMC 2 cut(s) 47, 1539
BalI TGGCCA 2 cut(s) 71, 237
BanI GGYRCC 1 cut(s) 2077
BanII GRGCYC 3 cut(s) 47, 488, 2557
BarI GAAGNNNNNNTAC 2 cut(s) 2893, 2925
BauI CACGAG 1 cut(s) 2445
BbrPI CACGTG 1 cut(s) 389
BbsI GAAGAC 2 cut(s) 931, 1919
Bbv12I GWGCWC 2 cut(s) 488, 2557
BbvI GCAGC 6 cut(s) 919, 1424, 1537, 1928, 2684, 2763
BccI CCATC 7 cut(s) 80, 647, 746, 1289, 1481, 1928, 2068
BceAI ACGGC 3 cut(s) 1241, 1680, 2298
BcgI CGANNNNNNTGC 2 cut(s) 2639, 2673
BciT130I CCWGG 5 cut(s) 234, 2388, 2870, 3018, 3035
BciVI GTATCC 3 cut(s) 273, 1676, 1822
BcoDI GTCTC 6 cut(s) 223, 1046, 1228, 1866, 1945, 2422
BcuI ACTAGT 1 cut(s) 446
BfmI CTRYAG 3 cut(s) 549, 1410, 2802
BfoI RGCGCY 1 cut(s) 2314
BfuI GTATCC 3 cut(s) 273, 1676, 1822
BglI GCCNNNNNGGC 1 cut(s) 32
BglII AGATCT 2 cut(s) 2566, 3061
BisI GCNGC 7 cut(s) 933, 1413, 1551, 1917, 2673, 2752, 3126
BlsI GCNGC 7 cut(s) 934, 1414, 1552, 1918, 2674, 2753, 3127
BmcAI AGTACT 1 cut(s) 1135
Bme1390I CCNGG 5 cut(s) 234, 2388, 2870, 3018, 3035
Bme18I GGWCC 3 cut(s) 1183, 1202, 1241
BmeT110I CYCGRG 1 cut(s) 2406
BmgBI CACGTC 1 cut(s) 2762
BmgT120I GGNCC 5 cut(s) 43, 44, 1183, 1202, 1241
BmiI GGNNCC 6 cut(s) 45, 531, 1184, 1185, 2079, 2164
BmrFI CCNGG 5 cut(s) 234, 2388, 2870, 3018, 3035
BmrI ACTGGG 2 cut(s) 1629, 2339
BmsI GCATC 7 cut(s) 586, 679, 748, 997, 1513, 2867, 2894
BmuI ACTGGG 2 cut(s) 1629, 2339
BoxI GACNNNNGTC 1 cut(s) 2760
BpiI GAAGAC 2 cut(s) 931, 1919
BplI GAGNNNNNCTC 2 cut(s) 975, 1007
Bpu10I CCTNAGC 1 cut(s) 2556
BpuEI CTTGAG 3 cut(s) 1656, 2048, 3050
BsaAI YACGTR 1 cut(s) 389
BsaI GGTCTC 2 cut(s) 1046, 1945
BsaJI CCNNGG 5 cut(s) 266, 1153, 1244, 1346, 3033
Bsc4I CCNNNNNNNGG 8 cut(s) 951, 1155, 1211, 1652, 1702, 2351, 2626, 2988
Bse1I ACTGG 4 cut(s) 370, 1624, 2239, 2345
Bse3DI GCAATG 1 cut(s) 2483
BseBI CCWGG 5 cut(s) 234, 2388, 2870, 3018, 3035
BseDI CCNNGG 5 cut(s) 266, 1153, 1244, 1346, 3033
BseGI GGATG 8 cut(s) 24, 526, 538, 694, 775, 1528, 2240, 2998
BseLI CCNNNNNNNGG 8 cut(s) 951, 1155, 1211, 1652, 1702, 2351, 2626, 2988
BseMI GCAATG 1 cut(s) 2483
BseMII CTCAG 9 cut(s) 195, 264, 2143, 2195, 2298, 2388, 2487, 2570, 2589
BseNI ACTGG 4 cut(s) 370, 1624, 2239, 2345
BseSI GKGCMC 2 cut(s) 47, 1539
BseXI GCAGC 6 cut(s) 919, 1424, 1537, 1928, 2684, 2763
BsgI GTGCAG 3 cut(s) 699, 735, 1113
BshFI GGCC 7 cut(s) 45, 71, 237, 271, 393, 1371, 2138
BshNI GGYRCC 1 cut(s) 2077
BsiHKAI GWGCWC 2 cut(s) 488, 2557
BsiHKCI CYCGRG 1 cut(s) 2406
BslFI GGGAC 7 cut(s) 71, 697, 1169, 1345, 2341, 2527, 2752
BslI CCNNNNNNNGG 8 cut(s) 951, 1155, 1211, 1652, 1702, 2351, 2626, 2988
BsmAI GTCTC 6 cut(s) 223, 1046, 1228, 1866, 1945, 2422
BsmBI CGTCTC 1 cut(s) 2422
BsmFI GGGAC 7 cut(s) 71, 697, 1169, 1345, 2341, 2527, 2752
BsmI GAATGC 1 cut(s) 2318
BsnI GGCC 7 cut(s) 45, 71, 237, 271, 393, 1371, 2138
Bso31I GGTCTC 2 cut(s) 1046, 1945
BsoBI CYCGRG 1 cut(s) 2406
Bsp120I GGGCCC 1 cut(s) 43
Bsp1286I GDGCHC 4 cut(s) 47, 488, 1539, 2557
Bsp19I CCATGG 1 cut(s) 1244
BspACI CCGC 7 cut(s) 41, 1693, 2166, 3076, 3091, 3110, 3125
BspANI GGCC 7 cut(s) 45, 71, 237, 271, 393, 1371, 2138
BspCNI CTCAG 9 cut(s) 194, 265, 2142, 2196, 2299, 2387, 2486, 2569, 2590
BspHI TCATGA 1 cut(s) 745
BspLI GGNNCC 6 cut(s) 45, 531, 1184, 1185, 2079, 2164
BspMAI CTGCAG 1 cut(s) 1414
BspPI GGATC 6 cut(s) 635, 1332, 1444, 2362, 2398, 2486
BspQI GCTCTTC 1 cut(s) 403
BspT107I GGYRCC 1 cut(s) 2077
BspTNI GGTCTC 2 cut(s) 1046, 1945
BsrDI GCAATG 1 cut(s) 2483
BsrI ACTGG 4 cut(s) 370, 1624, 2239, 2345
BssECI CCNNGG 5 cut(s) 266, 1153, 1244, 1346, 3033
BssNAI GTATAC 1 cut(s) 3145
BssSI CACGAG 1 cut(s) 2445
BssT1I CCWWGG 3 cut(s) 1153, 1244, 1346
Bst1107I GTATAC 1 cut(s) 3145
Bst2BI CACGAG 1 cut(s) 2445
Bst2UI CCWGG 5 cut(s) 234, 2388, 2870, 3018, 3035
Bst4CI ACNGT 6 cut(s) 150, 1381, 1457, 1603, 2525, 3117
Bst6I CTCTTC 5 cut(s) 403, 1231, 2158, 2388, 3190
BstAPI GCANNNNNTGC 1 cut(s) 2058
BstBAI YACGTR 1 cut(s) 389
BstC8I GCNNGC 2 cut(s) 43, 2821
BstDSI CCRYGG 1 cut(s) 1244
BstEII GGTNACC 1 cut(s) 2727
BstF5I GGATG 8 cut(s) 24, 526, 538, 694, 775, 1528, 2240, 2998
BstH2I RGCGCY 1 cut(s) 2314
BstHHI GCGC 3 cut(s) 9, 1609, 2313
BstMAI GTCTC 6 cut(s) 223, 1046, 1228, 1866, 1945, 2422
BstMWI GCNNNNNNNGC 8 cut(s) 32, 77, 1745, 2043, 2058, 2075, 2408, 3013
BstNI CCWGG 5 cut(s) 234, 2388, 2870, 3018, 3035
BstNSI RCATGY 2 cut(s) 932, 1628
BstPAI GACNNNNGTC 1 cut(s) 2760
BstPI GGTNACC 1 cut(s) 2727
BstSCI CCNGG 5 cut(s) 232, 2386, 2868, 3016, 3033
BstSFI CTRYAG 3 cut(s) 549, 1410, 2802
BstSLI GKGCMC 2 cut(s) 47, 1539
BstV1I GCAGC 6 cut(s) 919, 1424, 1537, 1928, 2684, 2763
BstV2I GAAGAC 2 cut(s) 931, 1919
BstX2I RGATCY 4 cut(s) 640, 2354, 2566, 3061
BstXI CCANNNNNNTGG 2 cut(s) 877, 2926
BstYI RGATCY 4 cut(s) 640, 2354, 2566, 3061
BstZ17I GTATAC 1 cut(s) 3145
BsuI GTATCC 3 cut(s) 273, 1676, 1822
BsuRI GGCC 7 cut(s) 45, 71, 237, 271, 393, 1371, 2138
BtgI CCRYGG 1 cut(s) 1244
BtrI CACGTC 1 cut(s) 2762
BtsCI GGATG 8 cut(s) 24, 526, 538, 694, 775, 1528, 2240, 2998
BtsI GCAGTG 1 cut(s) 75
BtsIMutI CAGTG 7 cut(s) 75, 1184, 2232, 2329, 2919, 3103, 3122
Cac8I GCNNGC 2 cut(s) 43, 2821
CaiI CAGNNNCTG 2 cut(s) 644, 1455
CciI TCATGA 1 cut(s) 745
CfoI GCGC 3 cut(s) 9, 1609, 2313
Cfr13I GGNCC 5 cut(s) 43, 44, 1183, 1202, 1241
CseI GACGC 1 cut(s) 123
Csp6I GTAC 7 cut(s) 178, 866, 893, 1081, 1134, 1377, 3154
CspCI CAANNNNNGTGG 4 cut(s) 1528, 1563, 2478, 2513
CviQI GTAC 7 cut(s) 178, 866, 893, 1081, 1134, 1377, 3154
DraI TTTAAA 1 cut(s) 1993
DraIII CACNNNGTG 1 cut(s) 2447
DrdI GACNNNNNNGTC 2 cut(s) 1880, 2436
DseDI GACNNNNNNGTC 2 cut(s) 1880, 2436
EaeI YGGCCR 2 cut(s) 69, 235
Eam1104I CTCTTC 5 cut(s) 403, 1231, 2158, 2388, 3190
EarI CTCTTC 5 cut(s) 403, 1231, 2158, 2388, 3190
Ecl136II GAGCTC 2 cut(s) 486, 2555
Eco130I CCWWGG 3 cut(s) 1153, 1244, 1346
Eco147I AGGCCT 2 cut(s) 1371, 2138
Eco24I GRGCYC 3 cut(s) 47, 488, 2557
Eco31I GGTCTC 2 cut(s) 1046, 1945
Eco32I GATATC 1 cut(s) 1015
Eco47I GGWCC 3 cut(s) 1183, 1202, 1241
Eco53kI GAGCTC 2 cut(s) 486, 2555
Eco57I CTGAAG 8 cut(s) 125, 372, 459, 621, 1212, 1800, 2943, 3064
Eco72I CACGTG 1 cut(s) 389
Eco88I CYCGRG 1 cut(s) 2406
Eco91I GGTNACC 1 cut(s) 2727
EcoICRI GAGCTC 2 cut(s) 486, 2555
EcoO109I RGGNCCY 1 cut(s) 1183
EcoO65I GGTNACC 1 cut(s) 2727
EcoRI GAATTC 1 cut(s) 1215
EcoRII CCWGG 5 cut(s) 232, 2386, 2868, 3016, 3033
EcoRV GATATC 1 cut(s) 1015
EcoT14I CCWWGG 3 cut(s) 1153, 1244, 1346
EcoT22I ATGCAT 1 cut(s) 2887
EcoT38I GRGCYC 3 cut(s) 47, 488, 2557
ErhI CCWWGG 3 cut(s) 1153, 1244, 1346
Esp3I CGTCTC 1 cut(s) 2422
FalI AAGNNNNNCTT 8 cut(s) 463, 495, 898, 930, 1488, 1520, 2386, 2418
FaqI GGGAC 7 cut(s) 71, 697, 1169, 1345, 2341, 2527, 2752
FauI CCCGC 2 cut(s) 34, 1700
FblI GTMKAC 2 cut(s) 1501, 3144
Fnu4HI GCNGC 7 cut(s) 933, 1413, 1551, 1917, 2673, 2752, 3126
FokI GGATG 8 cut(s) 31, 525, 533, 701, 782, 1535, 2227, 3005
FriOI GRGCYC 3 cut(s) 47, 488, 2557
Fsp4HI GCNGC 7 cut(s) 933, 1413, 1551, 1917, 2673, 2752, 3126
FspI TGCGCA 1 cut(s) 8
GlaI GCGC 3 cut(s) 8, 1608, 2312
GluI GCNGC 7 cut(s) 933, 1413, 1551, 1917, 2673, 2752, 3126
HaeII RGCGCY 1 cut(s) 2314
HaeIII GGCC 7 cut(s) 45, 71, 237, 271, 393, 1371, 2138
HgaI GACGC 1 cut(s) 123
HhaI GCGC 3 cut(s) 9, 1609, 2313
Hin6I GCGC 3 cut(s) 7, 1607, 2311
HinP1I GCGC 3 cut(s) 7, 1607, 2311
HincII GTYRAC 2 cut(s) 1502, 1561
HindII GTYRAC 2 cut(s) 1502, 1561
HindIII AAGCTT 1 cut(s) 1469
HinfI GANTC 7 cut(s) 161, 857, 881, 990, 1174, 1358, 3054
HphI GGTGA 9 cut(s) 515, 767, 809, 983, 1121, 1304, 1526, 1839, 2409
Hpy188III TCNNGA 7 cut(s) 222, 746, 1773, 2394, 2406, 2427, 2591
HpyCH4III ACNGT 6 cut(s) 150, 1381, 1457, 1603, 2525, 3117
HpyCH4IV ACGT 4 cut(s) 388, 1056, 2761, 2793
HpyF10VI GCNNNNNNNGC 8 cut(s) 32, 77, 1745, 2043, 2058, 2075, 2408, 3013
HpySE526I ACGT 4 cut(s) 388, 1056, 2761, 2793
HspAI GCGC 3 cut(s) 7, 1607, 2311
KflI GGGWCCC 1 cut(s) 1183
LguI GCTCTTC 1 cut(s) 403
LmnI GCTCC 2 cut(s) 2168, 2290
Lsp1109I GCAGC 6 cut(s) 919, 1424, 1537, 1928, 2684, 2763
LweI GCATC 7 cut(s) 586, 679, 748, 997, 1513, 2867, 2894
MaeII ACGT 4 cut(s) 388, 1056, 2761, 2793
MaeIII GTNAC 7 cut(s) 93, 430, 940, 1096, 1109, 1261, 2727
MfeI CAATTG 4 cut(s) 1277, 2017, 2621, 2853
MflI RGATCY 4 cut(s) 640, 2354, 2566, 3061
MhlI GDGCHC 4 cut(s) 47, 488, 1539, 2557
MlsI TGGCCA 2 cut(s) 71, 237
MluNI TGGCCA 2 cut(s) 71, 237
MlyI GAGTC 5 cut(s) 851, 984, 1183, 1367, 3063
Mox20I TGGCCA 2 cut(s) 71, 237
Mph1103I ATGCAT 1 cut(s) 2887
MroXI GAANNNNTTC 3 cut(s) 165, 534, 2401
MscI TGGCCA 2 cut(s) 71, 237
MslI CAYNNNNRTG 4 cut(s) 543, 750, 1089, 2924
Msp20I TGGCCA 2 cut(s) 71, 237
MspA1I CMGCKG 1 cut(s) 554
MspR9I CCNGG 5 cut(s) 234, 2388, 2870, 3018, 3035
MunI CAATTG 4 cut(s) 1277, 2017, 2621, 2853
Mva1269I GAATGC 1 cut(s) 2318
MvaI CCWGG 5 cut(s) 234, 2388, 2870, 3018, 3035
MwoI GCNNNNNNNGC 8 cut(s) 32, 77, 1745, 2043, 2058, 2075, 2408, 3013
NcoI CCATGG 1 cut(s) 1244
NlaIV GGNNCC 6 cut(s) 45, 531, 1184, 1185, 2079, 2164
NmeAIII GCCGAG 1 cut(s) 247
NmuCI GTSAC 3 cut(s) 93, 1109, 1261
NsbI TGCGCA 1 cut(s) 8
NsiI ATGCAT 1 cut(s) 2887
NspI RCATGY 2 cut(s) 932, 1628
OliI CACNNNNGTG 1 cut(s) 2924
PaeR7I CTCGAG 1 cut(s) 2406
PagI TCATGA 1 cut(s) 745
PceI AGGCCT 2 cut(s) 1371, 2138
PciSI GCTCTTC 1 cut(s) 403
PcsI WCGNNNNNNNCGW 2 cut(s) 1381, 2581
PctI GAATGC 1 cut(s) 2318
PdmI GAANNNNTTC 3 cut(s) 165, 534, 2401
PfeI GAWTC 2 cut(s) 161, 881
PflFI GACNNNGTC 1 cut(s) 1593
PflMI CCANNNNNTGG 1 cut(s) 2351
PfoI TCCNGGA 1 cut(s) 2386
PkrI GCNGC 7 cut(s) 934, 1414, 1552, 1918, 2674, 2753, 3127
PleI GAGTC 5 cut(s) 851, 984, 1182, 1366, 3062
PmaCI CACGTG 1 cut(s) 389
PmlI CACGTG 1 cut(s) 389
PpsI GAGTC 5 cut(s) 851, 984, 1182, 1366, 3062
Ppu21I YACGTR 1 cut(s) 389
PpuMI RGGWCCY 1 cut(s) 1183
PshAI GACNNNNGTC 1 cut(s) 2760
PshBI ATTAAT 1 cut(s) 380
Psp124BI GAGCTC 2 cut(s) 488, 2557
Psp5II RGGWCCY 1 cut(s) 1183
Psp6I CCWGG 5 cut(s) 232, 2386, 2868, 3016, 3033
PspCI CACGTG 1 cut(s) 389
PspEI GGTNACC 1 cut(s) 2727
PspGI CCWGG 5 cut(s) 232, 2386, 2868, 3016, 3033
PspN4I GGNNCC 6 cut(s) 45, 531, 1184, 1185, 2079, 2164
PspOMI GGGCCC 1 cut(s) 43
PspPI GGNCC 5 cut(s) 43, 44, 1183, 1202, 1241
PspPPI RGGWCCY 1 cut(s) 1183
PsrI GAACNNNNNNTAC 2 cut(s) 885, 917
PstI CTGCAG 1 cut(s) 1414
PstNI CAGNNNCTG 2 cut(s) 644, 1455
PsuI RGATCY 4 cut(s) 640, 2354, 2566, 3061
PsyI GACNNNGTC 1 cut(s) 1593
PvuII CAGCTG 1 cut(s) 554
RsaI GTAC 7 cut(s) 179, 867, 894, 1082, 1135, 1378, 3155
RsaNI GTAC 7 cut(s) 178, 866, 893, 1081, 1134, 1377, 3154
RseI CAYNNNNRTG 4 cut(s) 543, 750, 1089, 2924
SacI GAGCTC 2 cut(s) 488, 2557
SalI GTCGAC 1 cut(s) 1500
SapI GCTCTTC 1 cut(s) 403
SatI GCNGC 7 cut(s) 933, 1413, 1551, 1917, 2673, 2752, 3126
Sau96I GGNCC 5 cut(s) 43, 44, 1183, 1202, 1241
ScaI AGTACT 1 cut(s) 1135
SchI GAGTC 5 cut(s) 851, 984, 1183, 1367, 3063
ScrFI CCNGG 5 cut(s) 234, 2388, 2870, 3018, 3035
SduI GDGCHC 4 cut(s) 47, 488, 1539, 2557
SfaNI GCATC 7 cut(s) 586, 679, 748, 997, 1513, 2867, 2894
SfcI CTRYAG 3 cut(s) 549, 1410, 2802
Sfr274I CTCGAG 1 cut(s) 2406
SinI GGWCC 3 cut(s) 1183, 1202, 1241
SlaI CTCGAG 1 cut(s) 2406
SmiMI CAYNNNNRTG 4 cut(s) 543, 750, 1089, 2924
SmlI CTYRAG 4 cut(s) 1671, 2063, 2406, 3065
SmoI CTYRAG 4 cut(s) 1671, 2063, 2406, 3065
SpeI ACTAGT 1 cut(s) 446
SseBI AGGCCT 2 cut(s) 1371, 2138
SsiI CCGC 7 cut(s) 41, 1693, 2166, 3076, 3091, 3110, 3125
SspI AATATT 1 cut(s) 2218
SstI GAGCTC 2 cut(s) 488, 2557
StuI AGGCCT 2 cut(s) 1371, 2138
StyD4I CCNGG 5 cut(s) 232, 2386, 2868, 3016, 3033
StyI CCWWGG 3 cut(s) 1153, 1244, 1346
TaaI ACNGT 6 cut(s) 150, 1381, 1457, 1603, 2525, 3117
TaiI ACGT 4 cut(s) 391, 1059, 2764, 2796
TatI WGTACW 3 cut(s) 892, 1133, 3153
TauI GCSGC 1 cut(s) 3128
TfiI GAWTC 2 cut(s) 161, 881
TscAI CASTG 7 cut(s) 82, 1184, 2239, 2329, 2926, 3103, 3122
TseFI GTSAC 3 cut(s) 93, 1109, 1261
TseI GCWGC 6 cut(s) 932, 1412, 1550, 1916, 2672, 2751
Tsp45I GTSAC 3 cut(s) 93, 1109, 1261
TspGWI ACGGA 3 cut(s) 701, 869, 1971
TspRI CASTG 7 cut(s) 82, 1184, 2239, 2329, 2926, 3103, 3122
Tth111I GACNNNGTC 1 cut(s) 1593
Van91I CCANNNNNTGG 1 cut(s) 2351
VpaK11BI GGWCC 3 cut(s) 1183, 1202, 1241
VspI ATTAAT 1 cut(s) 380
XapI RAATTY 6 cut(s) 131, 1061, 1215, 1720, 1841, 2932
XbaI TCTAGA 1 cut(s) 221
XceI RCATGY 2 cut(s) 932, 1628
XhoI CTCGAG 1 cut(s) 2406
XmiI GTMKAC 2 cut(s) 1501, 3144
XmnI GAANNNNTTC 3 cut(s) 165, 534, 2401
ZrmI AGTACT 1 cut(s) 1135
Zsp2I ATGCAT 1 cut(s) 2887
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.