MD02G1288400.v1.1

Protein present in Fab1, YOTB, Vac1, and EEA1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0003738
Physical Location & Seq
Forward (+)
34483139 .. 34491485
8347 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1288400.v1.1.491

Sequence Viewer

Length: 3237 bp
ATGCTGCGTATGGACAGGATGACTGCGGATCAGGCAAAGGCGGGCCCACTCGAAAGGGACATTGACTTGGCCATCACTGCTCTGAAAAAGGGGGCACAATTGCTCAAGTATGGACGCAGAGGGAAACCCAAATTTTGTCCTTTCAGGCTGTCCAATGATGAATCTTTTCTAATATGGTACTCAGGGAAAGAGGAGAAATATGTTAAACTAACCCAAGTCTCAAGAATTGTACCTGGTCAGCGCACTCAAACCTTTCAGAGGTATCCTCGGCCTGAAAAGGAATATCAGTCATTTTCTCTTATATATAATGATAGGTCTTTGGATTTGATATGCAAGGATAAAGATGAAGCTGAAGTTTGGTTTACTGGTCTAAAAGCATTAATATCACGTGGCCTTCACCAGAAGCGGAGAGCAGAATCAAGAAGTGGAGTTACATCTGAGGCAAATAGTCCTAGATCACACACCCAACAAAGTTCTCCATTGAGCTCTCCATTTTGTAGTGGTGATAGTTCGCAGAAGGATGGCGTGGAACCGTTTCATCTTCATGCTGCATTTGAAAGCCCTCCTAAAATTGGTTTAGAGAAGGCTTTATCGGATGTCATGTTGTATGCCCTGCCTCCAAAAATTCCCTTCCCTTCAGATGCTTCTTGTGGCTCAGTCCCGTCTTCGGGAGGCTCAGATGGAATGAACGGACGAACAAAGGGTGCCGGTGTGGATGCATTTAGAGTAAGTTTATCAAGTGCTGTTAGCTCATCAAGTCAAGGCTCTGGTCATGATGAAGGTGATGCTTTAGCTTTAGGGGATGTTTTCATTTGGGGGGAAGGCGTTGGTGATTGCTTTCTCGGTGGAGGATCACATAGAGTTGGAAGTTCTTCTGGTTGTAAGATGGATTCTTTTGTGCCTAAAGTCTTGGAATCTGCCGTAGTGCTTGATGTCCAAAGCATAGCATGTGGTGGACGGCATGCTGCTTTTGTAACCAAGCAAGGGGAGGTATTTTCTTGGGGTGAAGAATTAGGAGGCAGACTTGGACAAGGTGTGGATGCTGATGTTTCACATCCAAAACTCATAGATGCTCTGAAAAACATGAATATTGAATTATTAGCATGTGGAGAATACCATTCTTGTGCTGTCACACTTTCCGGTGACATGTACACATGGGGTGGCAGTAATTCCAATTTTGCACTCCTTGGTCATGGGTCTCAATCAAGTCAATGGGTCCCAAAAAAGCTAAATGGACCTTTAGAGGGAATTCATGTCTCATCGGTTTCATGTGGACCATGGCACACAGCTGTTGTGACCTCTGCTGGGCAACTGTTTACTTTTGGCGATGGCACATTTGGTGTTCTAGGTCATGGGGATCGAAAGAGCACTTCGGTGCCCAGGGAGGTTGATTCCCTTAAAGGTCTCCGCACTGTGCGAGTAGCTTGTGGTGTTTGGCACACTGCAGCAGTAATTGAAGTTATGGTGGGGTCATCAAGTTCCAGCAATTGCTCTTCTGGAAAGCTTTTTACATGGGGAGATGGAGATAAAGGTCGACTTGGACACGGTGATAAGGAAGCAAGACTTGTGCCCACTTGTGTTGCTGCTTTGGTTGAACCCAACTTCTGTAAAGTTGCCTGCGGACAAAGCATGACTGTTGCACTTACAACTACGGGCCATGTTTACACTATGGGAAGCCCTGTTTTTGGTCAATTAGGGGTCCCTAAAGCTGATGGGAAACTTCCAAGACGTGTCGAGGGAAAGCTAATGAAGAGTTTTGTTGAAGAAATTGCTTGTGGTGATAATCATGTTGCGGTTTTAACTTCAAGAACTGAAGTTTACACTTGGGGAAAGGGTGCAAATGGTCGATTAGGTCTTGGGGATACAGAAGACAAATTTTCACCAACACTAGTTGAAGCTTTAAAAGACAAACAAGTCAAGAGTATTGTCTGTGGTGCTAACTTTACTGCTGCTATCTGTCTTCACAAATGGGTCTCTGGTATTGATCAATCAATGTGTTCTGGCTGTCGCCTTCCCTTTAATTTTAAAAGAAAAAGACACAATTGTTATAATTGTGGACTTGTGCTATGCCATTCATGCAGCAGCAACAAGTGTCTCAAGGCGTCCATGGCACCAAACCCAAACAAACCTTATCGTGTCTGTGATAACTGTTTTGCAAAACTTAGGAAGGCCACTGAAACCAACTCTTCATCTGGGTCTGCTATCAGTAGAAGGGGAAGTATGAATCAGGGATTCAACGAACTGATTGAAAACAATGTTAAAGAAATGGAAAATGGATCTTCCAAAAGCAACAAGAAAATGGATTTCAGCAGCAATTGTGTTACTCCCATTGCAAATGGAGTTTCTGAGCGTCGCGCACTCAATGGGACTAAATCTTTCAACCCTGGATCATCCAAAAAGTTCTTCTCTGCTTCGCTTCCTGGATCCAGAATTGCATCTCGAGCAACATCACCAACATCGAGACGATCTAGTCCACCTCGTTCCACAACACCAACCCCAGCTCTTTCAGGGCTTACTTTTCCAAAAATTGGGGATGATGCTAAAAGGAAAAATGTCAATCTAAGTGAAGAAGTTCTAAAATTAAAAGCTCAGGTTGAAGATCTTACTCATAAAGCTCAACTTCAAGACATTGAGATGGAAAAAACTACCCAACAGTTGAAGGAGGCTCTGAAAGTAGCAGGGGAAGAAACTCTGAAATGCAAAGCAGCGAAGGAAGTAATTAAGTCACTCACAGCCCAATTAAAGGAAATGGCTGAAAGGTTACCAATTGGAACGGCAAGAAACATGGATACCCGTCCGTTAGACTCTTCCAGTCCCACTCATGAAGTTTCTACTACAGCCGTCGAGAAAACAAGCAGTCTATTGAATTTCCATGAACCTTATTCAGTTGTATCGGACAGTGTGATGATTTCTGGTGTGCCACGGGCCACTTCGAATCACACAGAAGTGTCACATTCAGAAGCAACAGCTAGGCATAAAAACAGAGGAACAAAGACTGAAATAGCACAAGGGGACGAGTGGGTTGAGCAAGATGAGCCTGGTGTATATATTACCCTTGTTTCCTTGCCTGGAGGTGTCAAAGATCTCAAGCGTGTTCGTTTCAGTCGAAAGCGGTTCAGCGAGAAACAAGCAGAACAGTGGTGGGCGGCAAATAGGGGTAGAGTATATCAGCAGTACAATGTTCCGGTGGTTGAAAAATCCAGTATTCCGATGGGGAGAGAAGGGTTAGCTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BRX BRX_N BRX_assoc FYVE PH_12 RCC1

Protein Analysis

1079

Amino Acids

116.47

Weight (kDa)

9.05

Isoelectric Point (pI)

43.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH_12 PF16457 26 - 128 3.5e-08 Pleckstrin homology domain
WD40_RLD PF25390 268 - 651 4.8e-81 RCC1-like domain
RCC1_2 PF13540 312 - 336 2.3e-06 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 328 - 377 7.9e-10 Regulator of chromosome condensation (RCC1) repeat
Alsin_RLD PF25384 328 - 440 5.7e-07 Alsin RCC1-like domain
RCC1_2 PF13540 367 - 390 1.5e-06 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 380 - 432 2.1e-09 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 419 - 447 8.3e-06 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 436 - 484 6.6e-14 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 499 - 548 1.4e-13 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 552 - 600 4.6e-07 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 605 - 652 8.7e-15 Regulator of chromosome condensation (RCC1) repeat
FYVE PF01363 656 - 722 2.1e-14 FYVE zinc finger
BRX_N PF13713 895 - 925 1e-13 Transcription factor BRX N-terminal domain
BRX PF08381 1005 - 1059 2.5e-28 Transcription factor regulating root and shoot growth via Pin3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2049
AasI GACNNNNNNGTC 2 cut(s) 1913, 2469
AccB1I GGYRCC 3 cut(s) 704, 1375, 2110
AccB7I CCANNNNNTGG 1 cut(s) 2528
AccI GTMKAC 1 cut(s) 1534
AccII CGCG 1 cut(s) 2355
AciI CCGC 8 cut(s) 26, 41, 406, 1408, 1620, 1793, 3112, 3146
AclWI GGATC 7 cut(s) 36, 859, 1365, 2284, 2395, 2418, 2431
AcoI YGGCCR 1 cut(s) 69
AcsI RAATTY 5 cut(s) 131, 624, 1248, 1874, 2866
AcuI CTGAAG 3 cut(s) 372, 621, 1833
AcvI CACGTG 1 cut(s) 389
AcyI GRCGYC 1 cut(s) 2102
AfaI GTAC 4 cut(s) 179, 231, 1151, 3176
AflIII ACRYGT 2 cut(s) 1146, 1729
AhlI ACTAGT 1 cut(s) 1888
AjiI CACGTC 1 cut(s) 1730
AjnI CCWGG 6 cut(s) 232, 1379, 2383, 2419, 3037, 3067
AjuI GAANNNNNNNTTGG 2 cut(s) 2387, 2419
AleI CACNNNNGTG 2 cut(s) 1373, 2945
Alw21I GWGCWC 2 cut(s) 488, 1370
Alw26I GTCTC 7 cut(s) 223, 1203, 1261, 1409, 1978, 2099, 2455
AlwI GGATC 7 cut(s) 36, 859, 1365, 2284, 2395, 2418, 2431
Ama87I CYCGRG 1 cut(s) 2439
AoxI GGCC 7 cut(s) 43, 69, 269, 391, 1654, 2169, 2925
ApaI GGGCCC 1 cut(s) 47
ApoI RAATTY 5 cut(s) 131, 624, 1248, 1874, 2866
AseI ATTAAT 1 cut(s) 380
Asp700I GAANNNNTTC 4 cut(s) 165, 534, 871, 2571
AspLEI GCGC 2 cut(s) 243, 2357
AspS9I GGNCC 8 cut(s) 43, 44, 1216, 1235, 1274, 1654, 1699, 2925
AsuII TTCGAA 1 cut(s) 2933
AvaI CYCGRG 1 cut(s) 2439
AvaII GGWCC 4 cut(s) 1216, 1235, 1274, 1699
BaeGI GKGCMC 4 cut(s) 47, 97, 1380, 1572
BalI TGGCCA 1 cut(s) 71
BamHI GGATCC 1 cut(s) 2423
BanI GGYRCC 3 cut(s) 704, 1375, 2110
BanII GRGCYC 2 cut(s) 47, 488
BarI GAAGNNNNNNTAC 2 cut(s) 415, 447
BbrPI CACGTG 1 cut(s) 389
BbsI GAAGAC 3 cut(s) 657, 1875, 1952
Bbv12I GWGCWC 2 cut(s) 488, 1370
BbvI GCAGC 9 cut(s) 535, 952, 1457, 1570, 1936, 2091, 2094, 2322, 2717
BccI CCATC 9 cut(s) 80, 515, 674, 880, 1322, 1514, 1706, 2629, 3205
BceAI ACGGC 4 cut(s) 905, 974, 2790, 2825
BciT130I CCWGG 6 cut(s) 234, 1381, 2385, 2421, 3039, 3069
BciVI GTATCC 3 cut(s) 273, 1855, 2782
BclI TGATCA 1 cut(s) 1984
BcoDI GTCTC 7 cut(s) 223, 1203, 1261, 1409, 1978, 2099, 2455
BcuI ACTAGT 1 cut(s) 1888
BfaI CTAG 5 cut(s) 453, 1346, 1889, 2469, 2970
BfmI CTRYAG 2 cut(s) 1443, 2835
BfuI GTATCC 3 cut(s) 273, 1855, 2782
BglII AGATCT 2 cut(s) 2599, 3082
Bme1390I CCNGG 6 cut(s) 234, 1381, 2385, 2421, 3039, 3069
Bme18I GGWCC 4 cut(s) 1216, 1235, 1274, 1699
BmeT110I CYCGRG 1 cut(s) 2439
BmgBI CACGTC 1 cut(s) 1730
BmgT120I GGNCC 8 cut(s) 43, 44, 1216, 1235, 1274, 1654, 1699, 2925
BmrFI CCNGG 6 cut(s) 234, 1381, 2385, 2421, 3039, 3069
BmsI GCATC 7 cut(s) 631, 706, 775, 1030, 1060, 2444, 2527
BpiI GAAGAC 3 cut(s) 657, 1875, 1952
BplI GAGNNNNNCTC 2 cut(s) 250, 282
BpmI CTGGAG 1 cut(s) 3090
Bpu10I CCTNAGC 1 cut(s) 2589
Bpu14I TTCGAA 1 cut(s) 2933
BpuEI CTTGAG 4 cut(s) 89, 205, 2081, 3071
BsaAI YACGTR 1 cut(s) 389
BsaHI GRCGYC 1 cut(s) 2102
BsaI GGTCTC 3 cut(s) 1203, 1409, 1978
BsaJI CCNNGG 8 cut(s) 266, 1186, 1277, 1379, 1380, 2106, 2383, 2921
BsaWI WCCGGW 2 cut(s) 1139, 3184
Bse118I RCCGGY 1 cut(s) 707
Bse1I ACTGG 3 cut(s) 370, 2811, 3201
Bse3DI GCAATG 1 cut(s) 2328
BseBI CCWGG 6 cut(s) 234, 1381, 2385, 2421, 3039, 3069
BseDI CCNNGG 8 cut(s) 266, 1186, 1277, 1379, 1380, 2106, 2383, 2921
BseGI GGATG 9 cut(s) 24, 526, 601, 721, 808, 1045, 1054, 2390, 2539
BseMI GCAATG 1 cut(s) 2328
BseMII CTCAG 6 cut(s) 195, 429, 669, 690, 2337, 2603
BseNI ACTGG 3 cut(s) 370, 2811, 3201
BseRI GAGGAG 1 cut(s) 206
BseSI GKGCMC 4 cut(s) 47, 97, 1380, 1572
BseXI GCAGC 9 cut(s) 535, 952, 1457, 1570, 1936, 2091, 2094, 2322, 2717
BseYI CCCAGC 2 cut(s) 1304, 2497
Bsh1236I CGCG 1 cut(s) 2355
BshFI GGCC 7 cut(s) 45, 71, 271, 393, 1656, 2171, 2927
BshNI GGYRCC 3 cut(s) 704, 1375, 2110
BsiHKAI GWGCWC 2 cut(s) 488, 1370
BsiHKCI CYCGRG 1 cut(s) 2439
BsiSI CCGG 3 cut(s) 708, 1140, 3185
BslFI GGGAC 7 cut(s) 71, 644, 1202, 1685, 2380, 2799, 3026
BsmAI GTCTC 7 cut(s) 223, 1203, 1261, 1409, 1978, 2099, 2455
BsmBI CGTCTC 1 cut(s) 2455
BsmFI GGGAC 7 cut(s) 71, 644, 1202, 1685, 2380, 2799, 3026
BsnI GGCC 7 cut(s) 45, 71, 271, 393, 1656, 2171, 2927
Bso31I GGTCTC 3 cut(s) 1203, 1409, 1978
BsoBI CYCGRG 1 cut(s) 2439
Bsp119I TTCGAA 1 cut(s) 2933
Bsp120I GGGCCC 1 cut(s) 43
Bsp1286I GDGCHC 6 cut(s) 47, 97, 488, 1370, 1380, 1572
Bsp1407I TGTACA 1 cut(s) 1149
Bsp19I CCATGG 2 cut(s) 1277, 2106
BspACI CCGC 8 cut(s) 26, 41, 406, 1408, 1620, 1793, 3112, 3146
BspANI GGCC 7 cut(s) 45, 71, 271, 393, 1656, 2171, 2927
BspCNI CTCAG 6 cut(s) 194, 430, 668, 689, 2338, 2602
BspFNI CGCG 1 cut(s) 2355
BspHI TCATGA 2 cut(s) 772, 2821
BspMAI CTGCAG 1 cut(s) 1447
BspPI GGATC 7 cut(s) 36, 859, 1365, 2284, 2395, 2418, 2431
BspQI GCTCTTC 1 cut(s) 1498
BspT104I TTCGAA 1 cut(s) 2933
BspT107I GGYRCC 3 cut(s) 704, 1375, 2110
BspTNI GGTCTC 3 cut(s) 1203, 1409, 1978
BsrDI GCAATG 1 cut(s) 2328
BsrFI RCCGGY 1 cut(s) 707
BsrGI TGTACA 1 cut(s) 1149
BsrI ACTGG 3 cut(s) 370, 2811, 3201
BssAI RCCGGY 1 cut(s) 707
BssECI CCNNGG 8 cut(s) 266, 1186, 1277, 1379, 1380, 2106, 2383, 2921
BssNI GRCGYC 1 cut(s) 2102
BssT1I CCWWGG 3 cut(s) 1186, 1277, 2106
Bst2UI CCWGG 6 cut(s) 234, 1381, 2385, 2421, 3039, 3069
Bst4CI ACNGT 9 cut(s) 534, 1314, 1414, 1547, 1636, 2150, 2655, 2900, 3138
Bst6I CTCTTC 4 cut(s) 1498, 1745, 2191, 2812
BstACI GRCGYC 1 cut(s) 2102
BstAUI TGTACA 1 cut(s) 1149
BstBAI YACGTR 1 cut(s) 389
BstBI TTCGAA 1 cut(s) 2933
BstC8I GCNNGC 3 cut(s) 43, 963, 1618
BstDEI CTNAG 8 cut(s) 181, 438, 655, 676, 2162, 2346, 2561, 2589
BstDSI CCRYGG 3 cut(s) 1277, 2106, 2921
BstEII GGTNACC 1 cut(s) 2760
BstENI CCTNNNNNAGG 1 cut(s) 256
BstF5I GGATG 9 cut(s) 24, 526, 601, 721, 808, 1045, 1054, 2390, 2539
BstFNI CGCG 1 cut(s) 2355
BstHHI GCGC 2 cut(s) 243, 2357
BstMAI GTCTC 7 cut(s) 223, 1203, 1261, 1409, 1978, 2099, 2455
BstMWI GCNNNNNNNGC 7 cut(s) 32, 77, 1626, 2076, 2108, 2441, 3034
BstNI CCWGG 6 cut(s) 234, 1381, 2385, 2421, 3039, 3069
BstNSI RCATGY 4 cut(s) 951, 965, 1107, 1150
BstPI GGTNACC 1 cut(s) 2760
BstSCI CCNGG 6 cut(s) 232, 1379, 2383, 2419, 3037, 3067
BstSFI CTRYAG 2 cut(s) 1443, 2835
BstSLI GKGCMC 4 cut(s) 47, 97, 1380, 1572
BstUI CGCG 1 cut(s) 2355
BstV1I GCAGC 9 cut(s) 535, 952, 1457, 1570, 1936, 2091, 2094, 2322, 2717
BstV2I GAAGAC 3 cut(s) 657, 1875, 1952
BstX2I RGATCY 4 cut(s) 2276, 2423, 2599, 3082
BstYI RGATCY 4 cut(s) 2276, 2423, 2599, 3082
BsuI GTATCC 3 cut(s) 273, 1855, 2782
BsuRI GGCC 7 cut(s) 45, 71, 271, 393, 1656, 2171, 2927
BtgI CCRYGG 3 cut(s) 1277, 2106, 2921
BtgZI GCGATG 1 cut(s) 1341
BtrI CACGTC 1 cut(s) 1730
BtsCI GGATG 9 cut(s) 24, 526, 601, 721, 808, 1045, 1054, 2390, 2539
BtsI GCAGTG 2 cut(s) 75, 1440
BtsIMutI CAGTG 6 cut(s) 75, 1410, 1440, 2172, 2905, 3143
Cac8I GCNNGC 3 cut(s) 43, 963, 1618
CciI TCATGA 2 cut(s) 772, 2821
CfoI GCGC 2 cut(s) 243, 2357
Cfr10I RCCGGY 1 cut(s) 707
Cfr13I GGNCC 8 cut(s) 43, 44, 1216, 1235, 1274, 1654, 1699, 2925
CseI GACGC 3 cut(s) 123, 2091, 2339
CsiI ACCWGGT 1 cut(s) 232
Csp6I GTAC 4 cut(s) 178, 230, 1150, 3175
CspCI CAANNNNNGTGG 2 cut(s) 1561, 1596
CviQI GTAC 4 cut(s) 178, 230, 1150, 3175
DdeI CTNAG 8 cut(s) 181, 438, 655, 676, 2162, 2346, 2561, 2589
DraI TTTAAA 2 cut(s) 1902, 2026
DrdI GACNNNNNNGTC 2 cut(s) 1913, 2469
DseDI GACNNNNNNGTC 2 cut(s) 1913, 2469
EaeI YGGCCR 1 cut(s) 69
Eam1104I CTCTTC 4 cut(s) 1498, 1745, 2191, 2812
EarI CTCTTC 4 cut(s) 1498, 1745, 2191, 2812
Ecl136II GAGCTC 1 cut(s) 486
Eco130I CCWWGG 3 cut(s) 1186, 1277, 2106
Eco24I GRGCYC 2 cut(s) 47, 488
Eco31I GGTCTC 3 cut(s) 1203, 1409, 1978
Eco47I GGWCC 4 cut(s) 1216, 1235, 1274, 1699
Eco53kI GAGCTC 1 cut(s) 486
Eco57I CTGAAG 3 cut(s) 372, 621, 1833
Eco72I CACGTG 1 cut(s) 389
Eco88I CYCGRG 1 cut(s) 2439
Eco91I GGTNACC 1 cut(s) 2760
EcoICRI GAGCTC 1 cut(s) 486
EcoNI CCTNNNNNAGG 1 cut(s) 256
EcoO109I RGGNCCY 2 cut(s) 1216, 1699
EcoO65I GGTNACC 1 cut(s) 2760
EcoRI GAATTC 1 cut(s) 1248
EcoRII CCWGG 6 cut(s) 232, 1379, 2383, 2419, 3037, 3067
EcoT14I CCWWGG 3 cut(s) 1186, 1277, 2106
EcoT22I ATGCAT 1 cut(s) 721
EcoT38I GRGCYC 2 cut(s) 47, 488
ErhI CCWWGG 3 cut(s) 1186, 1277, 2106
Esp3I CGTCTC 1 cut(s) 2455
FalI AAGNNNNNCTT 6 cut(s) 1521, 1553, 1548, 1580, 2604, 2636
FaqI GGGAC 7 cut(s) 71, 644, 1202, 1685, 2380, 2799, 3026
FauI CCCGC 1 cut(s) 34
FbaI TGATCA 1 cut(s) 1984
FblI GTMKAC 1 cut(s) 1534
FokI GGATG 9 cut(s) 31, 533, 608, 728, 815, 1041, 1052, 2377, 2546
FriOI GRGCYC 2 cut(s) 47, 488
FspBI CTAG 5 cut(s) 453, 1346, 1889, 2469, 2970
GlaI GCGC 2 cut(s) 242, 2356
GsaI CCCAGC 2 cut(s) 1308, 2501
GsuI CTGGAG 1 cut(s) 3090
HaeIII GGCC 7 cut(s) 45, 71, 271, 393, 1656, 2171, 2927
HapII CCGG 3 cut(s) 708, 1140, 3185
HgaI GACGC 3 cut(s) 123, 2091, 2339
HhaI GCGC 2 cut(s) 243, 2357
Hin1I GRCGYC 1 cut(s) 2102
Hin6I GCGC 2 cut(s) 241, 2355
HinP1I GCGC 2 cut(s) 241, 2355
HincII GTYRAC 1 cut(s) 1535
HindII GTYRAC 1 cut(s) 1535
HindIII AAGCTT 2 cut(s) 1502, 1896
HinfI GANTC 9 cut(s) 161, 416, 890, 914, 1391, 2224, 2232, 2804, 2935
HpaII CCGG 3 cut(s) 708, 1140, 3185
Hpy99I CGWCG 2 cut(s) 2355, 2846
HpyCH4III ACNGT 9 cut(s) 534, 1314, 1414, 1547, 1636, 2150, 2655, 2900, 3138
HpyCH4IV ACGT 2 cut(s) 388, 1729
HpyF10VI GCNNNNNNNGC 7 cut(s) 32, 77, 1626, 2076, 2108, 2441, 3034
HpyF3I CTNAG 8 cut(s) 181, 438, 655, 676, 2162, 2346, 2561, 2589
HpySE526I ACGT 2 cut(s) 388, 1729
Hsp92I GRCGYC 1 cut(s) 2102
HspAI GCGC 2 cut(s) 241, 2355
KflI GGGWCCC 2 cut(s) 1216, 1699
Ksp22I TGATCA 1 cut(s) 1984
LguI GCTCTTC 1 cut(s) 1498
Lsp1109I GCAGC 9 cut(s) 535, 952, 1457, 1570, 1936, 2091, 2094, 2322, 2717
LweI GCATC 7 cut(s) 631, 706, 775, 1030, 1060, 2444, 2527
MabI ACCWGGT 1 cut(s) 232
MaeI CTAG 5 cut(s) 453, 1346, 1889, 2469, 2970
MaeII ACGT 2 cut(s) 388, 1729
MaeIII GTNAC 9 cut(s) 430, 973, 1129, 1142, 1294, 2320, 2724, 2760, 2949
MfeI CAATTG 5 cut(s) 98, 1486, 2041, 2314, 2766
MflI RGATCY 4 cut(s) 2276, 2423, 2599, 3082
MhlI GDGCHC 6 cut(s) 47, 97, 488, 1370, 1380, 1572
MlsI TGGCCA 1 cut(s) 71
MluNI TGGCCA 1 cut(s) 71
MlyI GAGTC 1 cut(s) 2798
MmeI TCCRAC 1 cut(s) 844
Mox20I TGGCCA 1 cut(s) 71
Mph1103I ATGCAT 1 cut(s) 721
MroXI GAANNNNTTC 4 cut(s) 165, 534, 871, 2571
MscI TGGCCA 1 cut(s) 71
MslI CAYNNNNRTG 5 cut(s) 543, 1122, 1373, 2633, 2945
Msp20I TGGCCA 1 cut(s) 71
MspA1I CMGCKG 1 cut(s) 1289
MspI CCGG 3 cut(s) 708, 1140, 3185
MspR9I CCNGG 6 cut(s) 234, 1381, 2385, 2421, 3039, 3069
MunI CAATTG 5 cut(s) 98, 1486, 2041, 2314, 2766
MvaI CCWGG 6 cut(s) 234, 1381, 2385, 2421, 3039, 3069
MvnI CGCG 1 cut(s) 2355
MwoI GCNNNNNNNGC 7 cut(s) 32, 77, 1626, 2076, 2108, 2441, 3034
NcoI CCATGG 2 cut(s) 1277, 2106
NmeAIII GCCGAG 1 cut(s) 247
NmuCI GTSAC 5 cut(s) 1129, 1142, 1294, 2724, 2949
NsiI ATGCAT 1 cut(s) 721
NspI RCATGY 4 cut(s) 951, 965, 1107, 1150
NspV TTCGAA 1 cut(s) 2933
OliI CACNNNNGTG 2 cut(s) 1373, 2945
PaeI GCATGC 1 cut(s) 965
PaeR7I CTCGAG 1 cut(s) 2439
PagI TCATGA 2 cut(s) 772, 2821
PasI CCCWGGG 1 cut(s) 1380
PciI ACATGT 1 cut(s) 1146
PciSI GCTCTTC 1 cut(s) 1498
PcsI WCGNNNNNNNCGW 1 cut(s) 3103
PdmI GAANNNNTTC 4 cut(s) 165, 534, 871, 2571
PfeI GAWTC 8 cut(s) 161, 416, 890, 914, 1391, 2224, 2232, 2935
PflMI CCANNNNNTGG 1 cut(s) 2528
PfoI TCCNGGA 1 cut(s) 2419
PleI GAGTC 1 cut(s) 2798
PmaCI CACGTG 1 cut(s) 389
PmlI CACGTG 1 cut(s) 389
PpsI GAGTC 1 cut(s) 2798
Ppu21I YACGTR 1 cut(s) 389
PpuMI RGGWCCY 2 cut(s) 1216, 1699
PscI ACATGT 1 cut(s) 1146
PshBI ATTAAT 1 cut(s) 380
PsiI TTATAA 1 cut(s) 2049
Psp124BI GAGCTC 1 cut(s) 488
Psp5II RGGWCCY 2 cut(s) 1216, 1699
Psp6I CCWGG 6 cut(s) 232, 1379, 2383, 2419, 3037, 3067
PspCI CACGTG 1 cut(s) 389
PspEI GGTNACC 1 cut(s) 2760
PspFI CCCAGC 2 cut(s) 1304, 2497
PspGI CCWGG 6 cut(s) 232, 1379, 2383, 2419, 3037, 3067
PspOMI GGGCCC 1 cut(s) 43
PspPI GGNCC 8 cut(s) 43, 44, 1216, 1235, 1274, 1654, 1699, 2925
PspPPI RGGWCCY 2 cut(s) 1216, 1699
PstI CTGCAG 1 cut(s) 1447
PsuI RGATCY 4 cut(s) 2276, 2423, 2599, 3082
PvuII CAGCTG 1 cut(s) 1289
RsaI GTAC 4 cut(s) 179, 231, 1151, 3176
RsaNI GTAC 4 cut(s) 178, 230, 1150, 3175
RseI CAYNNNNRTG 5 cut(s) 543, 1122, 1373, 2633, 2945
SacI GAGCTC 1 cut(s) 488
SalI GTCGAC 1 cut(s) 1533
SapI GCTCTTC 1 cut(s) 1498
Sau96I GGNCC 8 cut(s) 43, 44, 1216, 1235, 1274, 1654, 1699, 2925
SchI GAGTC 1 cut(s) 2798
ScrFI CCNGG 6 cut(s) 234, 1381, 2385, 2421, 3039, 3069
SduI GDGCHC 6 cut(s) 47, 97, 488, 1370, 1380, 1572
SexAI ACCWGGT 1 cut(s) 232
SfaNI GCATC 7 cut(s) 631, 706, 775, 1030, 1060, 2444, 2527
SfcI CTRYAG 2 cut(s) 1443, 2835
Sfr274I CTCGAG 1 cut(s) 2439
SfuI TTCGAA 1 cut(s) 2933
SinI GGWCC 4 cut(s) 1216, 1235, 1274, 1699
SlaI CTCGAG 1 cut(s) 2439
SmiMI CAYNNNNRTG 5 cut(s) 543, 1122, 1373, 2633, 2945
SmlI CTYRAG 5 cut(s) 104, 220, 2096, 2439, 3086
SmoI CTYRAG 5 cut(s) 104, 220, 2096, 2439, 3086
SpeI ACTAGT 1 cut(s) 1888
SphI GCATGC 1 cut(s) 965
SsiI CCGC 8 cut(s) 26, 41, 406, 1408, 1620, 1793, 3112, 3146
SspI AATATT 1 cut(s) 1090
SspMI CTAG 5 cut(s) 453, 1346, 1889, 2469, 2970
SstI GAGCTC 1 cut(s) 488
StyD4I CCNGG 6 cut(s) 232, 1379, 2383, 2419, 3037, 3067
StyI CCWWGG 3 cut(s) 1186, 1277, 2106
TaaI ACNGT 9 cut(s) 534, 1314, 1414, 1547, 1636, 2150, 2655, 2900, 3138
TaiI ACGT 2 cut(s) 391, 1732
TatI WGTACW 2 cut(s) 1149, 3174
TauI GCSGC 1 cut(s) 3149
TfiI GAWTC 8 cut(s) 161, 416, 890, 914, 1391, 2224, 2232, 2935
TscAI CASTG 6 cut(s) 82, 1417, 1447, 2179, 2905, 3143
TseFI GTSAC 5 cut(s) 1129, 1142, 1294, 2724, 2949
Tsp45I GTSAC 5 cut(s) 1129, 1142, 1294, 2724, 2949
TspGWI ACGGA 2 cut(s) 705, 2787
TspRI CASTG 6 cut(s) 82, 1417, 1447, 2179, 2905, 3143
Van91I CCANNNNNTGG 1 cut(s) 2528
VpaK11BI GGWCC 4 cut(s) 1216, 1235, 1274, 1699
VspI ATTAAT 1 cut(s) 380
XagI CCTNNNNNAGG 1 cut(s) 256
XapI RAATTY 5 cut(s) 131, 624, 1248, 1874, 2866
XceI RCATGY 4 cut(s) 951, 965, 1107, 1150
XcmI CCANNNNNNNNNTGG 1 cut(s) 3208
XhoI CTCGAG 1 cut(s) 2439
XmiI GTMKAC 1 cut(s) 1534
XmnI GAANNNNTTC 4 cut(s) 165, 534, 871, 2571
XspI CTAG 5 cut(s) 453, 1346, 1889, 2469, 2970
Zsp2I ATGCAT 1 cut(s) 721
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.