AT5G18390

PPR repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005891
Physical Location & Seq
Forward (+)
6090891 .. 6092657
1767 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G18390.1

Sequence Viewer

Length: 1380 bp
ATGTTACTCCTTCGTCGTTATTCCGGCGGAAGTCTTTACTCGATCCTCCCTATCAGAGCCTCTATTGAATCTACTATTCGTCATTTCAACAGCCTCGAGCCTTTGCAAAGCTCTGATTCGACTCCAACTAAAGGCGATTACTTCGCCGCGATCAACCACGTTGTCAATATCGTCCGCCGCGAGATTCACCCGGAGAGATCATTGAACAGTCTCCGTCTACCTGTAACCTCCGAATTCGTGTTCAGAGTCCTCCGCGCAACTTCTCGTTCCTCCAACGATTCTCTCCGATTCTTCAATTGGGCTCGATCGAACCCTAGCTACACACCAACCTCCATGGAATATGAAGAGCTTGCTAAGTCCTTAGCTTCACATAAAAAATACGAATCGATGTGGAAGATCCTCAAACAGATGAAGGATTTGTCTCTCGACATCTCCGGTGAGACACTCTGCTTCATCATTGAGCAATACGGTAAGAACGGTCATGTAGATCAAGCCGTAGAGCTTTTCAACGGCGTCCCGAAGACTCTAGGATGTCAACAAACCGTTGATGTTTACAATTCCTTGCTCCATGCGCTTTGTGATGTGAAGATGTTCCATGGTGCTTATGCTTTGATTCGGCGAATGATTAGGAAAGGTCTCAAGCCTGATAAGCGGACATACGCTATCTTGGTTAACGGATGGTGTTCCGCTGGAAAAATGAAGGAGGCTCAAGAGTTTCTTGATGAAATGAGTAGGAGAGGGTTTAATCCACCGGCTCGTGGTCGTGATTTGCTTATTGAAGGTTTGTTGAATGCTGGTTACTTGGAATCTGCTAAAGAGATGGTGAGTAAGATGACCAAAGGAGGGTTTGTTCCTGATATTCAAACTTTTAATATTTTGATTGAAGCTATATCTAAATCAGGGGAAGTTGAATTTTGCATTGAGATGTATTATACAGCTTGCAAGTTAGGTCTTTGTGTAGATATTGATACTTACAAGACACTAATACCTGCGGTTTCAAAGATTGGAAAGATTGATGAAGCGTTTCGGTTGTTGAACAATTGTGTGGAAGATGGGCATAAGCCGTTTCCGAGTTTGTATGCTCCGATAATCAAAGGAATGTGTAGGAATGGGATGTTTGATGATGCATTTAGCTTTTTTAGTGACATGAAGGTGAAGGCTCATCCGCCTAACAGGCCGGTTTATACAATGCTGATTACAATGTGTGGTCGTGGTGGGAAATTTGTGGATGCGGCTAATTACTTGGTTGAGATGACAGAGATGGGTTTGGTTCCGATATCACGGTGCTTTGATATGGTTACTGATGGGTTGAAGAATGGCGGGAAGCATGATTTGGCTATGCGAATAGAGCAGTTGGAAGTTCAGCTTAGAGGAGTTTGA

Protein Analysis

459

Amino Acids

51.77

Weight (kDa)

8.71

Isoelectric Point (pI)

45.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 182 - 228 1.1e-11 PPR repeat family
TPR_24 PF23276 207 - 311 1.3e-07 Fungal tetratrico peptide repeats
PPR_1 PF12854 211 - 244 1e-12 PPR repeat
PPR PF01535 219 - 248 1e-05 PPR repeat
PPR_2 PF13041 258 - 297 1e-07 PPR repeat family
PPR_1 PF12854 360 - 384 3.5e-06 PPR repeat
PPR_2 PF13041 362 - 403 9.3e-10 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 997
AccI GTMKAC 1 cut(s) 217
AccII CGCG 3 cut(s) 149, 180, 255
AclWI GGATC 2 cut(s) 37, 391
AcsI RAATTY 3 cut(s) 233, 911, 1220
AcyI GRCGYC 1 cut(s) 513
AfiI CCNNNNNNNGG 3 cut(s) 131, 758, 843
AjuI GAANNNNNNNTTGG 2 cut(s) 1316, 1348
AloI GAACNNNNNNTCC 2 cut(s) 834, 866
AluBI AGCT 9 cut(s) 111, 318, 349, 365, 502, 887, 938, 1134, 1366
AluI AGCT 9 cut(s) 111, 318, 349, 365, 502, 887, 938, 1134, 1366
Alw26I GTCTC 4 cut(s) 215, 426, 434, 641
AlwI GGATC 2 cut(s) 37, 391
Ama87I CYCGRG 1 cut(s) 95
AoxI GGCC 1 cut(s) 1175
ApoI RAATTY 3 cut(s) 233, 911, 1220
Asp700I GAANNNNTTC 2 cut(s) 590, 1023
AspLEI GCGC 2 cut(s) 257, 574
AsuC2I CCSGG 1 cut(s) 191
AsuHPI GGTGA 4 cut(s) 179, 449, 835, 1165
AvaI CYCGRG 1 cut(s) 95
BanII GRGCYC 1 cut(s) 304
BauI CACGAG 1 cut(s) 756
BbsI GAAGAC 1 cut(s) 527
BccI CCATC 5 cut(s) 672, 814, 1046, 1255, 1298
BceAI ACGGC 3 cut(s) 479, 526, 1048
BcnI CCSGG 1 cut(s) 191
BcoDI GTCTC 4 cut(s) 215, 426, 434, 641
BfaI CTAG 2 cut(s) 315, 527
BfuAI ACCTGC 1 cut(s) 997
BglI GCCNNNNNGGC 1 cut(s) 1174
BisI GCNGC 3 cut(s) 147, 178, 1233
BlsI GCNGC 3 cut(s) 148, 179, 1234
Bme1390I CCNGG 1 cut(s) 191
BmeT110I CYCGRG 1 cut(s) 95
BmiI GGNNCC 1 cut(s) 1272
BmrFI CCNGG 1 cut(s) 191
BmsI GCATC 2 cut(s) 1114, 1219
BpiI GAAGAC 1 cut(s) 527
Bpu10I CCTNAGC 1 cut(s) 361
BpuEI CTTGAG 2 cut(s) 623, 693
BpuMI CCSGG 1 cut(s) 191
Bsa29I ATCGAT 1 cut(s) 386
BsaHI GRCGYC 1 cut(s) 513
BsaI GGTCTC 1 cut(s) 641
BsaJI CCNNGG 2 cut(s) 333, 595
BsaWI WCCGGW 1 cut(s) 434
BsaXI ACNNNNNCTCC 2 cut(s) 834, 864
Bsc4I CCNNNNNNNGG 3 cut(s) 131, 758, 843
Bse118I RCCGGY 2 cut(s) 751, 1177
BseCI ATCGAT 1 cut(s) 386
BseDI CCNNGG 2 cut(s) 333, 595
BseGI GGATG 5 cut(s) 536, 683, 1119, 1162, 1234
BseLI CCNNNNNNNGG 3 cut(s) 131, 758, 843
Bsh1236I CGCG 3 cut(s) 149, 180, 255
Bsh1285I CGRYCG 1 cut(s) 308
BshFI GGCC 1 cut(s) 1177
BshVI ATCGAT 1 cut(s) 386
BsiEI CGRYCG 1 cut(s) 308
BsiHKCI CYCGRG 1 cut(s) 95
BsiSI CCGG 5 cut(s) 24, 191, 435, 752, 1178
BslFI GGGAC 1 cut(s) 500
BslI CCNNNNNNNGG 3 cut(s) 131, 758, 843
BsmAI GTCTC 4 cut(s) 215, 426, 434, 641
BsmFI GGGAC 1 cut(s) 500
BsmI GAATGC 1 cut(s) 796
BsnI GGCC 1 cut(s) 1177
Bso31I GGTCTC 1 cut(s) 641
BsoBI CYCGRG 1 cut(s) 95
Bsp1286I GDGCHC 1 cut(s) 304
Bsp143I GATC 6 cut(s) 42, 150, 197, 305, 396, 487
Bsp19I CCATGG 2 cut(s) 333, 595
BspANI GGCC 1 cut(s) 1177
BspDI ATCGAT 1 cut(s) 386
BspFNI CGCG 3 cut(s) 149, 180, 255
BspLI GGNNCC 1 cut(s) 1272
BspMI ACCTGC 1 cut(s) 997
BspPI GGATC 2 cut(s) 37, 391
BspQI GCTCTTC 1 cut(s) 339
BspTNI GGTCTC 1 cut(s) 641
BsrFI RCCGGY 2 cut(s) 751, 1177
BssAI RCCGGY 2 cut(s) 751, 1177
BssECI CCNNGG 2 cut(s) 333, 595
BssMI GATC 6 cut(s) 42, 150, 197, 305, 396, 487
BssNI GRCGYC 1 cut(s) 513
BssSI CACGAG 1 cut(s) 756
BssT1I CCWWGG 2 cut(s) 333, 595
Bst2BI CACGAG 1 cut(s) 756
Bst4CI ACNGT 5 cut(s) 209, 470, 479, 544, 1284
Bst6I CTCTTC 1 cut(s) 339
BstACI GRCGYC 1 cut(s) 513
BstC8I GCNNGC 2 cut(s) 351, 940
BstDEI CTNAG 3 cut(s) 354, 361, 1367
BstDSI CCRYGG 2 cut(s) 333, 595
BstF5I GGATG 5 cut(s) 536, 683, 1119, 1162, 1234
BstFNI CGCG 3 cut(s) 149, 180, 255
BstHHI GCGC 2 cut(s) 257, 574
BstKTI GATC 6 cut(s) 45, 153, 200, 308, 399, 490
BstMAI GTCTC 4 cut(s) 215, 426, 434, 641
BstMBI GATC 6 cut(s) 42, 150, 197, 305, 396, 487
BstMCI CGRYCG 1 cut(s) 308
BstMWI GCNNNNNNNGC 4 cut(s) 571, 649, 1174, 1348
BstSCI CCNGG 1 cut(s) 189
BstUI CGCG 3 cut(s) 149, 180, 255
BstV2I GAAGAC 1 cut(s) 527
BstX2I RGATCY 1 cut(s) 396
BstYI RGATCY 1 cut(s) 396
Bsu15I ATCGAT 1 cut(s) 386
BsuRI GGCC 1 cut(s) 1177
BsuTUI ATCGAT 1 cut(s) 386
BtgI CCRYGG 2 cut(s) 333, 595
BtsCI GGATG 5 cut(s) 536, 683, 1119, 1162, 1234
BveI ACCTGC 1 cut(s) 997
Cac8I GCNNGC 2 cut(s) 351, 940
CfoI GCGC 2 cut(s) 257, 574
Cfr10I RCCGGY 2 cut(s) 751, 1177
ClaI ATCGAT 1 cut(s) 386
CseI GACGC 1 cut(s) 502
CviAII CATG 6 cut(s) 334, 482, 569, 596, 1147, 1328
DdeI CTNAG 3 cut(s) 354, 361, 1367
DpnI GATC 6 cut(s) 44, 152, 199, 307, 398, 489
DpnII GATC 6 cut(s) 42, 150, 197, 305, 396, 487
Eam1104I CTCTTC 1 cut(s) 339
EarI CTCTTC 1 cut(s) 339
EciI GGCGGA 3 cut(s) 42, 164, 1155
Eco130I CCWWGG 2 cut(s) 333, 595
Eco24I GRGCYC 1 cut(s) 304
Eco31I GGTCTC 1 cut(s) 641
Eco32I GATATC 1 cut(s) 1278
Eco88I CYCGRG 1 cut(s) 95
EcoRI GAATTC 1 cut(s) 233
EcoRV GATATC 1 cut(s) 1278
EcoT14I CCWWGG 2 cut(s) 333, 595
EcoT22I ATGCAT 1 cut(s) 1129
EcoT38I GRGCYC 1 cut(s) 304
ErhI CCWWGG 2 cut(s) 333, 595
FaeI CATG 6 cut(s) 337, 485, 572, 599, 1150, 1331
FalI AAGNNNNNCTT 3 cut(s) 702, 734, 1350
FaqI GGGAC 1 cut(s) 500
FatI CATG 6 cut(s) 333, 481, 568, 595, 1146, 1327
FauI CCCGC 1 cut(s) 1313
FblI GTMKAC 1 cut(s) 217
Fnu4HI GCNGC 3 cut(s) 147, 178, 1233
FokI GGATG 5 cut(s) 543, 690, 1126, 1149, 1241
FriOI GRGCYC 1 cut(s) 304
Fsp4HI GCNGC 3 cut(s) 147, 178, 1233
FspBI CTAG 2 cut(s) 315, 527
GlaI GCGC 2 cut(s) 256, 573
GluI GCNGC 3 cut(s) 147, 178, 1233
HaeIII GGCC 1 cut(s) 1177
HapII CCGG 5 cut(s) 24, 191, 435, 752, 1178
HgaI GACGC 1 cut(s) 502
HhaI GCGC 2 cut(s) 257, 574
Hin1I GRCGYC 1 cut(s) 513
Hin1II CATG 6 cut(s) 337, 485, 572, 599, 1150, 1331
Hin6I GCGC 2 cut(s) 255, 572
HinP1I GCGC 2 cut(s) 255, 572
HincII GTYRAC 2 cut(s) 536, 673
HindII GTYRAC 2 cut(s) 536, 673
HpaI GTTAAC 1 cut(s) 673
HpaII CCGG 5 cut(s) 24, 191, 435, 752, 1178
HphI GGTGA 4 cut(s) 179, 449, 835, 1165
Hpy166II GTNNAC 4 cut(s) 218, 536, 553, 673
Hpy188I TCNGA 8 cut(s) 56, 115, 232, 245, 287, 1071, 1086, 1275
Hpy188III TCNNGA 6 cut(s) 425, 517, 710, 719, 764, 854
Hpy8I GTNNAC 4 cut(s) 218, 536, 553, 673
Hpy99I CGWCG 1 cut(s) 18
HpyAV CCTTC 6 cut(s) 20, 406, 694, 773, 1144, 1150
HpyCH4III ACNGT 5 cut(s) 209, 470, 479, 544, 1284
HpyCH4IV ACGT 1 cut(s) 159
HpyCH4V TGCA 4 cut(s) 106, 918, 942, 1127
HpyF10VI GCNNNNNNNGC 4 cut(s) 571, 649, 1174, 1348
HpyF3I CTNAG 3 cut(s) 354, 361, 1367
HpySE526I ACGT 1 cut(s) 159
Hsp92I GRCGYC 1 cut(s) 513
Hsp92II CATG 6 cut(s) 337, 485, 572, 599, 1150, 1331
HspAI GCGC 2 cut(s) 255, 572
KspAI GTTAAC 1 cut(s) 673
Kzo9I GATC 6 cut(s) 42, 150, 197, 305, 396, 487
LguI GCTCTTC 1 cut(s) 339
LmnI GCTCC 2 cut(s) 570, 1087
LweI GCATC 2 cut(s) 1114, 1219
MaeI CTAG 2 cut(s) 315, 527
MaeII ACGT 1 cut(s) 159
MaeIII GTNAC 5 cut(s) 3, 223, 797, 1142, 1297
MalI GATC 6 cut(s) 44, 152, 199, 307, 398, 489
MboI GATC 6 cut(s) 42, 150, 197, 305, 396, 487
MboII GAAGA 7 cut(s) 283, 356, 406, 532, 598, 1061, 1324
MfeI CAATTG 2 cut(s) 295, 1039
MflI RGATCY 1 cut(s) 396
MhlI GDGCHC 1 cut(s) 304
MluCI AATT 7 cut(s) 233, 295, 556, 911, 1039, 1220, 1237
MlyI GAGTC 3 cut(s) 115, 255, 517
MmeI TCCRAC 3 cut(s) 149, 297, 1335
Mph1103I ATGCAT 1 cut(s) 1129
MroXI GAANNNNTTC 2 cut(s) 590, 1023
MseI TTAA 3 cut(s) 672, 744, 870
MslI CAYNNNNRTG 2 cut(s) 923, 1151
MspA1I CMGCKG 1 cut(s) 689
MspI CCGG 5 cut(s) 24, 191, 435, 752, 1178
MspR9I CCNGG 1 cut(s) 191
MunI CAATTG 2 cut(s) 295, 1039
Mva1269I GAATGC 1 cut(s) 796
MvnI CGCG 3 cut(s) 149, 180, 255
MwoI GCNNNNNNNGC 4 cut(s) 571, 649, 1174, 1348
NciI CCSGG 1 cut(s) 191
NcoI CCATGG 2 cut(s) 333, 595
NdeII GATC 6 cut(s) 42, 150, 197, 305, 396, 487
NlaIII CATG 6 cut(s) 337, 485, 572, 599, 1150, 1331
NlaIV GGNNCC 1 cut(s) 1272
NmuCI GTSAC 1 cut(s) 1142
NsiI ATGCAT 1 cut(s) 1129
PaeR7I CTCGAG 1 cut(s) 95
PciSI GCTCTTC 1 cut(s) 339
PcsI WCGNNNNNNNCGW 1 cut(s) 177
PctI GAATGC 1 cut(s) 796
PdmI GAANNNNTTC 2 cut(s) 590, 1023
PfeI GAWTC 8 cut(s) 68, 116, 184, 278, 288, 383, 613, 806
PkrI GCNGC 3 cut(s) 148, 179, 1234
Ple19I CGATCG 1 cut(s) 308
PleI GAGTC 3 cut(s) 115, 254, 517
PpsI GAGTC 3 cut(s) 115, 254, 517
PspN4I GGNNCC 1 cut(s) 1272
PspXI VCTCGAGB 1 cut(s) 95
PsrI GAACNNNNNNTAC 2 cut(s) 302, 334
PsuI RGATCY 1 cut(s) 396
PvuI CGATCG 1 cut(s) 308
RseI CAYNNNNRTG 2 cut(s) 923, 1151
SapI GCTCTTC 1 cut(s) 339
SaqAI TTAA 3 cut(s) 672, 744, 870
SatI GCNGC 3 cut(s) 147, 178, 1233
Sau3AI GATC 6 cut(s) 42, 150, 197, 305, 396, 487
SchI GAGTC 3 cut(s) 115, 255, 517
ScrFI CCNGG 1 cut(s) 191
SduI GDGCHC 1 cut(s) 304
SfaNI GCATC 2 cut(s) 1114, 1219
Sfr274I CTCGAG 1 cut(s) 95
SlaI CTCGAG 1 cut(s) 95
SmiMI CAYNNNNRTG 2 cut(s) 923, 1151
SmlI CTYRAG 3 cut(s) 95, 638, 708
SmoI CTYRAG 3 cut(s) 95, 638, 708
Sse9I AATT 7 cut(s) 233, 295, 556, 911, 1039, 1220, 1237
SspI AATATT 1 cut(s) 874
SspMI CTAG 2 cut(s) 315, 527
StyD4I CCNGG 1 cut(s) 189
StyI CCWWGG 2 cut(s) 333, 595
TaaI ACNGT 5 cut(s) 209, 470, 479, 544, 1284
TaiI ACGT 1 cut(s) 162
TaqI TCGA 7 cut(s) 41, 96, 119, 304, 308, 386, 426
TasI AATT 7 cut(s) 233, 295, 556, 911, 1039, 1220, 1237
TauI GCSGC 3 cut(s) 149, 180, 1235
TfiI GAWTC 8 cut(s) 68, 116, 184, 278, 288, 383, 613, 806
Tru1I TTAA 3 cut(s) 672, 744, 870
Tru9I TTAA 3 cut(s) 672, 744, 870
TseFI GTSAC 1 cut(s) 1142
Tsp45I GTSAC 1 cut(s) 1142
TspDTI ATGAA 7 cut(s) 357, 425, 442, 713, 738, 1032, 1163
TspGWI ACGGA 2 cut(s) 203, 690
XapI RAATTY 3 cut(s) 233, 911, 1220
XhoI CTCGAG 1 cut(s) 95
XmiI GTMKAC 1 cut(s) 217
XmnI GAANNNNTTC 2 cut(s) 590, 1023
XspI CTAG 2 cut(s) 315, 527
Zsp2I ATGCAT 1 cut(s) 1129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.