pycom04g20890

Pentatricopeptide repeat-containing protein At5g18390

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0005891
Physical Location & Seq
Reverse (-)
22320324 .. 22321697
1374 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g20890.1

Sequence Viewer

Length: 1374 bp
ATGTTGGGCGCCGCCAAAAACTTACCGGTCCTCCACAGACCGCAACCACTCCGCCGCCTCCTCCGCCACTTCACCACCGCCCTTAATGCCGCCCCTCCAAACGGCGTCGTCCCGACAAAAGACGACTACTTCTCAGCCATCCATCACATCTCCAACATCGTCCGTCGCGACCACTACCTGGAGCGGACACTCAACAAGCTCCGAATCGCCGTCGACTCGGACCTCGTCTACCGCGTCCTCCGCGCCTGCTCCGGCCACGGCACCGAGTCGCTCCGATTCTTCAACTGGGCCCGGACCCACCACCCCACCTACCACCCCACGACCCTAGAGCTCGAAGAACTCGCCAAGATCCTCGCTCGCGCCAAAAAGTACGAATCGATGTGGAAGCTCCTCGAATCGATGCAGACCCACCACGAATTAACGCTCTCGGAAGAAACCCTCTGCTTCGTGATCGAAGAGTACGGCCGCCACGGCCTGGTGGATCAGGCGGTGGAGCTTTTCAATCGGGCGCCGAAGACCTTCCGGTGCTCCCAATCCGCCGGAGTTTACAACGCTCTGCTGTTCGCCCTCTGCGAAGGGAAGCTCTTTCATGGGGCTTATGCTCTGGTGAGGAGGATGATCCGAAAAGGGGTTGTTCCGGATAAGAAGACGTATTCGATTTTGGTGAATGCTTGGTGCTCCAATGGGAAAATGCGGGAGGCCCAGTTGTTCTTGGAGGAGATGAGCAGCAAAGGGTTCAATCCGCCGGTTCGCGGCCGCGATCTTCTCGTCGAAGGGCTGCTCAACGCTGGCTACGTCGAGGCTGCGAAAGGGATGGTAAGGAAGATGGTGAAGGAAGGATTTGTGCCTGATGTTTCTACTTTTAATGCATTGATGGAGGCTATATGTAAATGTGGGGAGGTTGATTTTTGCATTGATTTGTATTGGGAAGCAAATGGTTTGGGGCTCTGTCCTGATATCAACACTTACAAGGTGTTGATTCCGGCGGTTTCGAAGGTGGGGAGGATTGATGATGCGTTTCGGCTTCTGCATAACTCGATTGAAGAGGGGCATAAGCCGTTTCCGAGCTTGTATGCTTCTATAATTAAGGGAATGTGCAGGAGGGGGCAGTTCGATGATGCATTTTGTTTCTTTAGTGAGATGAAGGTTAAGGGGCATCCTCCCAACAGGCCAGTGTATACCATGTTGATAACGATGGCTGGACGCGGAGGGCGGTTTGTTGAGGCTGCGAATTACTTGGTGGAAATGACTGAGATGGGGTTGATGCCGATTTCGCGGTGCTTTGATTTTGTTACTGATGGATTGAAGAACTGTGGGAAGCATGATATGGCTAACAGGATTGAACAATTGGAAGTTTCTCTCCGCGGCGCGTGA

Protein Analysis

458

Amino Acids

51.69

Weight (kDa)

8.84

Isoelectric Point (pI)

39.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 182 - 226 7.5e-10 PPR repeat family
TPR_24 PF23276 205 - 309 1.5e-06 Fungal tetratrico peptide repeats
PPR_1 PF12854 209 - 242 1.7e-09 PPR repeat
PPR_2 PF13041 256 - 297 7.4e-07 PPR repeat family
PPR_2 PF13041 283 - 330 1.3e-07 PPR repeat family
PPR_1 PF12854 357 - 382 5.6e-06 PPR repeat
PPR_2 PF13041 358 - 398 1.5e-10 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 8, 260, 508
AccB7I CCANNNNNTGG 2 cut(s) 178, 475
AccBSI CCGCTC 1 cut(s) 184
AccI GTMKAC 3 cut(s) 213, 228, 1178
AccIII TCCGGA 1 cut(s) 637
AclWI GGATC 3 cut(s) 343, 489, 613
AcoI YGGCCR 3 cut(s) 253, 463, 754
AcyI GRCGYC 3 cut(s) 9, 105, 509
AfaI GTAC 2 cut(s) 371, 461
AfiI CCNNNNNNNGG 5 cut(s) 101, 178, 475, 628, 752
AgeI ACCGGT 1 cut(s) 25
AgsI TTSAA 6 cut(s) 283, 502, 739, 1043, 1306, 1343
AjnI CCWGG 2 cut(s) 177, 474
AluBI AGCT 6 cut(s) 199, 331, 388, 496, 583, 1068
AluI AGCT 6 cut(s) 199, 331, 388, 496, 583, 1068
Alw21I GWGCWC 3 cut(s) 333, 530, 680
AlwI GGATC 3 cut(s) 343, 489, 613
Aor13HI TCCGGA 1 cut(s) 637
AoxI GGCC 7 cut(s) 253, 288, 463, 472, 699, 754, 1169
ApaI GGGCCC 1 cut(s) 292
ApeKI GCWGC 4 cut(s) 726, 778, 803, 1226
AsiGI ACCGGT 1 cut(s) 25
Asp700I GAANNNNTTC 1 cut(s) 518
AspLEI GCGC 5 cut(s) 11, 245, 362, 511, 1370
AspS9I GGNCC 6 cut(s) 28, 220, 288, 289, 294, 700
AsuC2I CCSGG 1 cut(s) 292
AsuHPI GGTGA 4 cut(s) 64, 619, 676, 841
AsuII TTCGAA 1 cut(s) 992
AvaII GGWCC 3 cut(s) 28, 220, 294
BaeGI GKGCMC 1 cut(s) 292
BanI GGYRCC 3 cut(s) 8, 260, 508
BanII GRGCYC 3 cut(s) 292, 333, 948
BbsI GAAGAC 2 cut(s) 521, 653
Bbv12I GWGCWC 3 cut(s) 333, 530, 680
BbvI GCAGC 4 cut(s) 738, 765, 790, 1213
BccI CCATC 8 cut(s) 146, 150, 808, 820, 868, 1189, 1249, 1292
BceAI ACGGC 6 cut(s) 118, 194, 274, 478, 487, 1042
BciT130I CCWGG 2 cut(s) 179, 476
BcnI CCSGG 1 cut(s) 292
BfaI CTAG 1 cut(s) 326
BfoI RGCGCY 2 cut(s) 12, 512
BglI GCCNNNNNGGC 1 cut(s) 471
Bme1390I CCNGG 3 cut(s) 179, 292, 476
Bme18I GGWCC 3 cut(s) 28, 220, 294
BmgT120I GGNCC 6 cut(s) 28, 220, 288, 289, 294, 700
BmiI GGNNCC 5 cut(s) 10, 262, 290, 296, 510
BmrFI CCNGG 3 cut(s) 179, 292, 476
BmrI ACTGGG 2 cut(s) 295, 697
BmsI GCATC 5 cut(s) 390, 1003, 1108, 1165, 1254
BmuI ACTGGG 2 cut(s) 295, 697
BpiI GAAGAC 2 cut(s) 521, 653
BpmI CTGGAG 1 cut(s) 200
Bpu14I TTCGAA 1 cut(s) 992
BpuMI CCSGG 1 cut(s) 292
Bsa29I ATCGAT 2 cut(s) 377, 398
BsaHI GRCGYC 3 cut(s) 9, 105, 509
BsaJI CCNNGG 3 cut(s) 256, 469, 1363
BsaWI WCCGGW 3 cut(s) 25, 522, 637
BsaXI ACNNNNNCTCC 4 cut(s) 15, 45, 689, 719
Bsc4I CCNNNNNNNGG 5 cut(s) 101, 178, 475, 628, 752
Bse118I RCCGGY 2 cut(s) 25, 745
Bse1I ACTGG 3 cut(s) 290, 703, 1172
BseAI TCCGGA 1 cut(s) 637
BseBI CCWGG 2 cut(s) 179, 476
BseCI ATCGAT 2 cut(s) 377, 398
BseDI CCNNGG 3 cut(s) 256, 469, 1363
BseGI GGATG 4 cut(s) 138, 621, 819, 1156
BseLI CCNNNNNNNGG 5 cut(s) 101, 178, 475, 628, 752
BseMII CTCAG 2 cut(s) 147, 1242
BseNI ACTGG 3 cut(s) 290, 703, 1172
BseRI GAGGAG 4 cut(s) 50, 380, 625, 731
BseSI GKGCMC 1 cut(s) 292
BseX3I CGGCCG 2 cut(s) 463, 754
BseXI GCAGC 4 cut(s) 738, 765, 790, 1213
BsgI GTGCAG 1 cut(s) 1117
Bsh1285I CGRYCG 2 cut(s) 466, 757
BshFI GGCC 7 cut(s) 255, 290, 465, 474, 701, 756, 1171
BshNI GGYRCC 3 cut(s) 8, 260, 508
BshTI ACCGGT 1 cut(s) 25
BshVI ATCGAT 2 cut(s) 377, 398
BsiEI CGRYCG 2 cut(s) 466, 757
BsiHKAI GWGCWC 3 cut(s) 333, 530, 680
BsiSI CCGG 8 cut(s) 26, 252, 292, 523, 540, 638, 746, 983
BslFI GGGAC 1 cut(s) 95
BslI CCNNNNNNNGG 5 cut(s) 101, 178, 475, 628, 752
BsmFI GGGAC 1 cut(s) 95
BsmI GAATGC 1 cut(s) 673
BsnI GGCC 7 cut(s) 255, 290, 465, 474, 701, 756, 1171
Bsp119I TTCGAA 1 cut(s) 992
Bsp120I GGGCCC 1 cut(s) 288
Bsp1286I GDGCHC 5 cut(s) 292, 333, 530, 680, 948
Bsp13I TCCGGA 1 cut(s) 637
Bsp143I GATC 5 cut(s) 348, 450, 481, 618, 760
Bsp68I TCGCGA 1 cut(s) 168
BspANI GGCC 7 cut(s) 255, 290, 465, 474, 701, 756, 1171
BspCNI CTCAG 2 cut(s) 146, 1243
BspDI ATCGAT 2 cut(s) 377, 398
BspEI TCCGGA 1 cut(s) 637
BspLI GGNNCC 5 cut(s) 10, 262, 290, 296, 510
BspPI GGATC 3 cut(s) 343, 489, 613
BspT104I TTCGAA 1 cut(s) 992
BspT107I GGYRCC 3 cut(s) 8, 260, 508
BsrBI CCGCTC 1 cut(s) 184
BsrFI RCCGGY 2 cut(s) 25, 745
BsrI ACTGG 3 cut(s) 290, 703, 1172
BssAI RCCGGY 2 cut(s) 25, 745
BssECI CCNNGG 3 cut(s) 256, 469, 1363
BssMI GATC 5 cut(s) 348, 450, 481, 618, 760
BssNAI GTATAC 1 cut(s) 1179
BssNI GRCGYC 3 cut(s) 9, 105, 509
Bst1107I GTATAC 1 cut(s) 1179
Bst2UI CCWGG 2 cut(s) 179, 476
Bst4CI ACNGT 1 cut(s) 1313
Bst6I CTCTTC 2 cut(s) 450, 1038
BstACI GRCGYC 3 cut(s) 9, 105, 509
BstBI TTCGAA 1 cut(s) 992
BstC8I GCNNGC 3 cut(s) 247, 358, 790
BstDEI CTNAG 2 cut(s) 133, 1251
BstDSI CCRYGG 3 cut(s) 256, 469, 1363
BstF5I GGATG 4 cut(s) 138, 621, 819, 1156
BstH2I RGCGCY 2 cut(s) 12, 512
BstHHI GCGC 5 cut(s) 11, 245, 362, 511, 1370
BstKTI GATC 5 cut(s) 351, 453, 484, 621, 763
BstMBI GATC 5 cut(s) 348, 450, 481, 618, 760
BstMCI CGRYCG 2 cut(s) 466, 757
BstMWI GCNNNNNNNGC 5 cut(s) 63, 86, 240, 471, 1273
BstNI CCWGG 2 cut(s) 179, 476
BstSCI CCNGG 3 cut(s) 177, 290, 474
BstSLI GKGCMC 1 cut(s) 292
BstV1I GCAGC 4 cut(s) 738, 765, 790, 1213
BstV2I GAAGAC 2 cut(s) 521, 653
BstX2I RGATCY 1 cut(s) 348
BstYI RGATCY 1 cut(s) 348
BstZ17I GTATAC 1 cut(s) 1179
BstZI CGGCCG 2 cut(s) 463, 754
Bsu15I ATCGAT 2 cut(s) 377, 398
BsuRI GGCC 7 cut(s) 255, 290, 465, 474, 701, 756, 1171
BsuTUI ATCGAT 2 cut(s) 377, 398
BtgI CCRYGG 3 cut(s) 256, 469, 1363
BtsCI GGATG 4 cut(s) 138, 621, 819, 1156
BtsIMutI CAGTG 1 cut(s) 1179
BtuMI TCGCGA 1 cut(s) 168
Cac8I GCNNGC 3 cut(s) 247, 358, 790
CciNI GCGGCCGC 1 cut(s) 754
CfoI GCGC 5 cut(s) 11, 245, 362, 511, 1370
Cfr10I RCCGGY 2 cut(s) 25, 745
Cfr13I GGNCC 6 cut(s) 28, 220, 288, 289, 294, 700
Cfr42I CCGCGG 1 cut(s) 1366
ClaI ATCGAT 2 cut(s) 377, 398
CseI GACGC 3 cut(s) 94, 223, 1212
Csp6I GTAC 2 cut(s) 370, 460
CspAI ACCGGT 1 cut(s) 25
CviAII CATG 3 cut(s) 590, 1183, 1322
CviQI GTAC 2 cut(s) 370, 460
DdeI CTNAG 2 cut(s) 133, 1251
DinI GGCGCC 2 cut(s) 10, 510
DpnI GATC 5 cut(s) 350, 452, 483, 620, 762
DpnII GATC 5 cut(s) 348, 450, 481, 618, 760
EaeI YGGCCR 3 cut(s) 253, 463, 754
EagI CGGCCG 2 cut(s) 463, 754
Eam1104I CTCTTC 2 cut(s) 450, 1038
EarI CTCTTC 2 cut(s) 450, 1038
EciI GGCGGA 4 cut(s) 41, 53, 526, 732
Ecl136II GAGCTC 1 cut(s) 331
EclXI CGGCCG 2 cut(s) 463, 754
Eco24I GRGCYC 3 cut(s) 292, 333, 948
Eco32I GATATC 1 cut(s) 958
Eco47I GGWCC 3 cut(s) 28, 220, 294
Eco52I CGGCCG 2 cut(s) 463, 754
Eco53kI GAGCTC 1 cut(s) 331
EcoICRI GAGCTC 1 cut(s) 331
EcoRII CCWGG 2 cut(s) 177, 474
EcoRV GATATC 1 cut(s) 958
EcoT22I ATGCAT 2 cut(s) 871, 1123
EcoT38I GRGCYC 3 cut(s) 292, 333, 948
EgeI GGCGCC 2 cut(s) 10, 510
EheI GGCGCC 2 cut(s) 10, 510
FaeI CATG 3 cut(s) 593, 1186, 1325
FaqI GGGAC 1 cut(s) 95
FatI CATG 3 cut(s) 589, 1182, 1321
FauI CCCGC 1 cut(s) 687
FblI GTMKAC 3 cut(s) 213, 228, 1178
FokI GGATG 4 cut(s) 125, 628, 826, 1143
FriOI GRGCYC 3 cut(s) 292, 333, 948
FspBI CTAG 1 cut(s) 326
GlaI GCGC 5 cut(s) 10, 244, 361, 510, 1369
GsuI CTGGAG 1 cut(s) 200
HaeII RGCGCY 2 cut(s) 12, 512
HaeIII GGCC 7 cut(s) 255, 290, 465, 474, 701, 756, 1171
HapII CCGG 8 cut(s) 26, 252, 292, 523, 540, 638, 746, 983
HgaI GACGC 3 cut(s) 94, 223, 1212
HhaI GCGC 5 cut(s) 11, 245, 362, 511, 1370
Hin1I GRCGYC 3 cut(s) 9, 105, 509
Hin1II CATG 3 cut(s) 593, 1186, 1325
Hin6I GCGC 5 cut(s) 9, 243, 360, 509, 1368
HinP1I GCGC 5 cut(s) 9, 243, 360, 509, 1368
HincII GTYRAC 1 cut(s) 214
HindII GTYRAC 1 cut(s) 214
HinfI GANTC 7 cut(s) 204, 215, 266, 276, 374, 395, 979
HpaII CCGG 8 cut(s) 26, 252, 292, 523, 540, 638, 746, 983
HphI GGTGA 4 cut(s) 64, 619, 676, 841
Hpy166II GTNNAC 4 cut(s) 214, 229, 547, 1179
Hpy188I TCNGA 6 cut(s) 203, 220, 275, 430, 623, 1065
Hpy188III TCNNGA 5 cut(s) 112, 167, 448, 638, 953
Hpy8I GTNNAC 4 cut(s) 214, 229, 547, 1179
Hpy99I CGWCG 5 cut(s) 110, 168, 215, 773, 800
HpyAV CCTTC 7 cut(s) 529, 569, 767, 826, 830, 988, 1138
HpyCH4III ACNGT 1 cut(s) 1313
HpyCH4IV ACGT 2 cut(s) 650, 795
HpyCH4V TGCA 6 cut(s) 403, 869, 912, 1030, 1098, 1121
HpyF10VI GCNNNNNNNGC 5 cut(s) 63, 86, 240, 471, 1273
HpyF3I CTNAG 2 cut(s) 133, 1251
HpySE526I ACGT 2 cut(s) 650, 795
Hsp92I GRCGYC 3 cut(s) 9, 105, 509
Hsp92II CATG 3 cut(s) 593, 1186, 1325
HspAI GCGC 5 cut(s) 9, 243, 360, 509, 1368
KasI GGCGCC 2 cut(s) 8, 508
Kpn2I TCCGGA 1 cut(s) 637
KspI CCGCGG 1 cut(s) 1366
Kzo9I GATC 5 cut(s) 348, 450, 481, 618, 760
LmnI GCTCC 8 cut(s) 181, 204, 254, 276, 393, 493, 533, 683
Lsp1109I GCAGC 4 cut(s) 738, 765, 790, 1213
LweI GCATC 5 cut(s) 390, 1003, 1108, 1165, 1254
MaeI CTAG 1 cut(s) 326
MaeII ACGT 2 cut(s) 650, 795
MaeIII GTNAC 1 cut(s) 1291
MalI GATC 5 cut(s) 350, 452, 483, 620, 762
MbiI CCGCTC 1 cut(s) 184
MboI GATC 5 cut(s) 348, 450, 481, 618, 760
MfeI CAATTG 1 cut(s) 1346
MflI RGATCY 1 cut(s) 348
MhlI GDGCHC 5 cut(s) 292, 333, 530, 680, 948
MluCI AATT 4 cut(s) 416, 1083, 1231, 1346
Mly113I GGCGCC 2 cut(s) 9, 509
MlyI GAGTC 2 cut(s) 209, 275
MmeI TCCRAC 1 cut(s) 177
Mph1103I ATGCAT 2 cut(s) 871, 1123
MroI TCCGGA 1 cut(s) 637
MroXI GAANNNNTTC 1 cut(s) 518
MseI TTAA 5 cut(s) 84, 419, 864, 1086, 1149
MspA1I CMGCKG 1 cut(s) 1365
MspI CCGG 8 cut(s) 26, 252, 292, 523, 540, 638, 746, 983
MspR9I CCNGG 3 cut(s) 179, 292, 476
MunI CAATTG 1 cut(s) 1346
Mva1269I GAATGC 1 cut(s) 673
MvaI CCWGG 2 cut(s) 179, 476
MwoI GCNNNNNNNGC 5 cut(s) 63, 86, 240, 471, 1273
NarI GGCGCC 2 cut(s) 9, 509
NciI CCSGG 1 cut(s) 292
NdeII GATC 5 cut(s) 348, 450, 481, 618, 760
NlaIII CATG 3 cut(s) 593, 1186, 1325
NlaIV GGNNCC 5 cut(s) 10, 262, 290, 296, 510
NotI GCGGCCGC 1 cut(s) 754
NruI TCGCGA 1 cut(s) 168
NsiI ATGCAT 2 cut(s) 871, 1123
NspV TTCGAA 1 cut(s) 992
PcsI WCGNNNNNNNCGW 4 cut(s) 165, 231, 570, 792
PctI GAATGC 1 cut(s) 673
PdmI GAANNNNTTC 1 cut(s) 518
PfeI GAWTC 5 cut(s) 204, 276, 374, 395, 979
PflFI GACNNNGTC 1 cut(s) 224
PflMI CCANNNNNTGG 2 cut(s) 178, 475
PinAI ACCGGT 1 cut(s) 25
PleI GAGTC 2 cut(s) 209, 274
PluTI GGCGCC 2 cut(s) 12, 512
PpsI GAGTC 2 cut(s) 209, 274
Psp124BI GAGCTC 1 cut(s) 333
Psp6I CCWGG 2 cut(s) 177, 474
PspGI CCWGG 2 cut(s) 177, 474
PspN4I GGNNCC 5 cut(s) 10, 262, 290, 296, 510
PspOMI GGGCCC 1 cut(s) 288
PspPI GGNCC 6 cut(s) 28, 220, 288, 289, 294, 700
PsuI RGATCY 1 cut(s) 348
PsyI GACNNNGTC 1 cut(s) 224
RruI TCGCGA 1 cut(s) 168
RsaI GTAC 2 cut(s) 371, 461
RsaNI GTAC 2 cut(s) 370, 460
SacI GAGCTC 1 cut(s) 333
SacII CCGCGG 1 cut(s) 1366
SalI GTCGAC 1 cut(s) 212
SaqAI TTAA 5 cut(s) 84, 419, 864, 1086, 1149
Sau3AI GATC 5 cut(s) 348, 450, 481, 618, 760
Sau96I GGNCC 6 cut(s) 28, 220, 288, 289, 294, 700
SchI GAGTC 2 cut(s) 209, 275
ScrFI CCNGG 3 cut(s) 179, 292, 476
SduI GDGCHC 5 cut(s) 292, 333, 530, 680, 948
SfaNI GCATC 5 cut(s) 390, 1003, 1108, 1165, 1254
SfiI GGCCNNNNNGGCC 1 cut(s) 471
SfoI GGCGCC 2 cut(s) 10, 510
Sfr303I CCGCGG 1 cut(s) 1366
SfuI TTCGAA 1 cut(s) 992
SgrBI CCGCGG 1 cut(s) 1366
SinI GGWCC 3 cut(s) 28, 220, 294
Sse9I AATT 4 cut(s) 416, 1083, 1231, 1346
SspDI GGCGCC 2 cut(s) 8, 508
SspMI CTAG 1 cut(s) 326
SstI GAGCTC 1 cut(s) 333
StyD4I CCNGG 3 cut(s) 177, 290, 474
TaaI ACNGT 1 cut(s) 1313
TaiI ACGT 2 cut(s) 653, 798
TasI AATT 4 cut(s) 416, 1083, 1231, 1346
TauI GCSGC 7 cut(s) 14, 57, 92, 468, 756, 759, 1368
TfiI GAWTC 5 cut(s) 204, 276, 374, 395, 979
Tru1I TTAA 5 cut(s) 84, 419, 864, 1086, 1149
Tru9I TTAA 5 cut(s) 84, 419, 864, 1086, 1149
TscAI CASTG 1 cut(s) 1179
TseI GCWGC 4 cut(s) 726, 778, 803, 1226
TspDTI ATGAA 2 cut(s) 578, 1157
TspGWI ACGGA 1 cut(s) 152
TspRI CASTG 1 cut(s) 1179
Tth111I GACNNNGTC 1 cut(s) 224
Van91I CCANNNNNTGG 2 cut(s) 178, 475
VpaK11BI GGWCC 3 cut(s) 28, 220, 294
XmiI GTMKAC 3 cut(s) 213, 228, 1178
XmnI GAANNNNTTC 1 cut(s) 518
XspI CTAG 1 cut(s) 326
Zsp2I ATGCAT 2 cut(s) 871, 1123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.