Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0003250

Physical Location & Seq

Genomic Coordinates Forward (+)

7998197 .. 8002848

Gene Structure 4652 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G23720.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2790 bp

ATGGCGGAACCTGAGAAGAAGCGAGATCAACCATTTTCTCAAGAAAAAGACGAAGAGAAAGATCTATACCTTGTTCACGACGAGCACGAGTCTCCTCTTCCTCTCACCGTCACTTCTCGTGTTTTGTACATGTTAGGAGATATAGCATCTGGTCCAGCTTATAGGTTTACTCAATGGTTGGATTTAGTTCGTAAACGAAGTGCTACTTACGGATCCTCTGGCTTCCCTCACCGCCTTCACCGCATCGATGATATGGTCACTAGTGCAGGAGAGAGGAATACTGATCCTAAAAGTCCACCAAGTAGGCAGAGTTCAGAGATTAGTTTGTGGGAAAGGCTTGGTAAAGCTTCTACTGTGGATATTGATTCGAGCTGTTTTTCATGGAATATGTTGTCTTCACTTCATCATACTGAACATAGTAGTAGCACTGATCATTCTGAAGAAGACCAGAGTAAACCACTTGAGGTTACTGTGAATTCTGGAGGAGTTGTTTTCTTTGCCCTGTTCAATAGTTCCAGCTCTGAAGATGCCTCTCGTAAGGAAGAAGCAGCAGTTATTAAATTTGCATCATCGAGGATGGCCACGCAATCAGAACGTCTAGGTTATGAGTTTTCCAAGTGGCTAGGGGTGCAGATTCCGCAGGCTAGAGTAATTCACAGCTGTAATCCTGAGTGGACACTGATCAAAGAAGCCACTGAAAAAGCACAAGCTAAAGCGACTTCGGAGGGGGACGAGGTTGGTGAAATGACATGTTCAGAACTTCTGGAAGCCCTTGAGCTCAGCCGTTGTCTTCTCTTAATGAGTTATGTTCATGGATGTCCAATGCTGGAGAGCATGAGTAGTTTTGAAACTGAAGAAAAAGCAGAGAGAGCTGCTGCAGCTCTAGGGAGAATCTTGGTGTTAGATCTAGTAATTAGGAATGAAGACAGATTACCGTGTCGTCAGCTGAGATGGCGTGGCAACCCGGCAAATCTGTTACTGACAGACAGAATTGTGTCTTCTGCAAAGCATCATGAATGTTCATTTGATGAAGCTTTTGATTCCGCCATCAAACGGTACCATCCAAAGGACTATAGATCCATTCAAAGGGAAAGAAGAGCTTCTTCCGTAGATAGCAGATCCAGGTTGAGTATCAGTGATCAAATGTTGGTCTCACAGGCTTCTGATTTTTCTGATATAACGGAATCACCTAGATCATATGATACTGGTTTAATGAGTCCAATGTCTGATAGATCAGTAGCTGCCGATTTTCACCTCGTTGCCATAGACTCGGGTGTTCCACGGCGGCCTCCTGCTGGAAAACGGGCAAGCGACCAAGAAATTTATCCAAGATTAGTTGAGTTACTCCTCAATAGTAGCCAATATTCTTCGAATTTGTTACATGAAATAACAGAAGGAAGTTTAGGATATCCACAGGCAGAAGATGGTGAAGAAACATCTAATGTTCGATCTGTAGTAACACCAGTTGTACGCGAGTTTCGAAATGGTTTCCGAGCTGGTCTCAGGGATTTGCAAGAGTTTCACATCTTCCTTGTCACCCTTCATCAGAAATTAGATGTGTTGCTTCGGGCATTTTTTAGTATGATGGACAAAACAATGTGTGCAGATTTTGACAGAGAAGACTTTGCTGTTCCTGAGTCACCTTCACATACTCATGGGCATGAGGTGAATCATTATCCATCTCCCTCAAAAGATCGGGTGCCTAGTGATAACAGCTCTGATCACAGTGAGTCAGATATGCAAAAATCAGTTCCTAGGACACCAAATTCAGAAAACAAAGAAGACGGGTCTTCGCCTAAGTCACGTGAAAGTTGGCACGGAAGATCCGGTAAAGGTGGAGAATCTCTTAGCAGTCAGCGTTTGGCAGCAAAGCTCCGTGACTTCCATAAGTTTGCAAAGGTTGATGCTGAGTCTAATAAAGAACTAGACCAGTGGAATGAAACACTGCGGAATGAAGTGATGAAACTTTGCCAAGAGAACGGTTTCAACACTGGTTTTTTTGAGGGCAGTGATAATAACAGTTGCACAGATGCTTATGAGCTAAAGGTTAGACTTGAGCATATTCTTGAGAGGATATCATTGATTAGCAAAGCTGCTAATACAGAGAAACCATCAATGATACAAGAAAATCTTTTTATCGGGGGAGGATTGGCTGCAAGGTCGATTTACACTCTTCAGCATTTAGGTATAACTCATGTTCTATGTTTGTGCGCTAATGAAATCGGGCAATCGGATACTCAATACCCGGATCTCTTCGAGTACCAAAACTTCTCAATAACCGATGATGAAGACTCAAATATCGAGAGCATCTTTCAAGAAGCTCTCGATTTCATCAAGCATGGCGAAGAAACTGGTGGCAAGATTTTAGTTCATTGCTTCGAAGGTCGGAGTAGAAGCGCAACAGTAGTGCTTGCTTACCTAATGCTCCAAAAGAAGCTCACACTTTTAGAAGCATGGAGCAAACTGAGGAAAGTTCATAGACGAGCTCAACCGAATGATGGATTTGCGCGGATCTTAATAAACCTCGACAAGAAATGTCACGGGAAAGTATCAATGGAGTGGAGACAGAGGAAACCGACAATGAAAGTGTGTCCGGTTTGTGGAAAGAACGCAGGTCTAAGCAGCAGTTCTTTGAAGCTTCATCTCCAGAAATCTCACAGGAAACTGTCTTCGGGAAGTGTTGACAGTGCAATGAACATGGAGATTCAAAAGGCTTTGGAGGCTCTTAAACTCAGCACTGGCCGTGGCTCAAGTGCTAGTTCCAATTCTTTCCAATCTCATCCTGGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000226 GO:0001101 GO:0003674 GO:0003824 GO:0004721 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007010 GO:0007017 GO:0008138 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009987 GO:0010033 GO:0010119 GO:0010468 GO:0016043 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019222 GO:0019538 GO:0030865 GO:0031122 GO:0033993 GO:0036211 GO:0042221 GO:0042578 GO:0043170 GO:0043412 GO:0043622 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0060255 GO:0065007 GO:0071704 GO:0071840 GO:0097305 GO:0097435 GO:0140096 GO:1901564 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Act-Frag_cataly DSPc

Protein Analysis

929

Amino Acids

104.23

Weight (kDa)

5.97

Isoelectric Point (pI)

55.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Act-Frag_cataly	PF09192	21 - 342	2.8e-83	Actin-fragmin kinase, catalytic
DSPc	PF00782	638 - 759	1.2e-23	Dual specificity phosphatase, catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0003250)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G23720 AT5G23720 AT5G23720
fragaria_vesca	FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220
malus_domestica	MD06G1132400.v1.1 MD14G1148800.v1.1
prunus_persica	Prupe.5G143400_v2.0.a1
pyrus_communis	pycom06g12400 pycom14g12360
rosa_chinensis	RchiOBHm_Chr4g0416471 RchiOBHm_Chr7g0188941 RchiOBHm_Chr7g0194331
rosa_laevigata	RLG00000004049 RLG00000004648
rosa_multiflora	Rmu_co8071116.1_g000001 Rmu_sc0007831.1_g000001 Rmu_sc0032883.1_g000001 Rmu_ssc0000113.1_g000006
rosa_roxburghii	Rroxscaffold_3G00265920
rosa_rugosa	Rorug06G0494800 Rorug06G0494900 Rorug06G0495000
rosa_samantha	Rh7AG099700 Rh7BG101800 Rh7CG103100 Rh7CG146600 Rh7DG101600 Rh7DG144200
rosa_wichuraiana	Rw7G008590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	2603
Acc65I	GGTACC	1 cut(s)	1056
AccB1I	GGYRCC	2 cut(s)	1056, 1699
AccII	CGCG	2 cut(s)	1473, 2509
AciI	CCGC	8 cut(s)	5, 232, 241, 638, 1044, 1285, 1948, 2509
AclWI	GGATC	8 cut(s)	207, 220, 278, 1071, 1113, 1818, 2256, 2519
AcoI	YGGCCR	2 cut(s)	579, 2740
AcsI	RAATTY	5 cut(s)	475, 560, 1320, 1372, 1765
AcuI	CTGAAG	4 cut(s)	459, 543, 873, 2159
AcvI	CACGTG	1 cut(s)	1805
AfaI	GTAC	4 cut(s)	128, 1058, 1470, 2261
AfiI	CCNNNNNNNGG	6 cut(s)	1053, 1066, 1086, 1295, 2498, 2600
AflIII	ACRYGT	2 cut(s)	129, 749
AgsI	TTSAA	7 cut(s)	508, 848, 1085, 1987, 2315, 2635, 2708
AhlI	ACTAGT	1 cut(s)	260
AjnI	CCWGG	2 cut(s)	1121, 2782
Alw21I	GWGCWC	3 cut(s)	87, 780, 2488
Alw26I	GTCTC	4 cut(s)	96, 1156, 1505, 2557
AlwI	GGATC	8 cut(s)	207, 220, 278, 1071, 1113, 1818, 2256, 2519
AlwNI	CAGNNNCTG	1 cut(s)	1241
Ama87I	CYCGRG	1 cut(s)	1270
AoxI	GGCC	3 cut(s)	579, 1286, 2740
ApeKI	GCWGC	9 cut(s)	548, 872, 875, 878, 1241, 1865, 2093, 2153, 2622
ApoI	RAATTY	5 cut(s)	475, 560, 1320, 1372, 1765
Asp700I	GAANNNNTTC	2 cut(s)	1099, 1982
Asp718I	GGTACC	1 cut(s)	1056
AspA2I	CCTAGG	1 cut(s)	1754
AspLEI	GCGC	3 cut(s)	2213, 2399, 2509
AspS9I	GGNCC	1 cut(s)	152
AsuC2I	CCSGG	2 cut(s)	965, 2246
AsuII	TTCGAA	3 cut(s)	1370, 1480, 2379
AvaI	CYCGRG	1 cut(s)	1270
AvaII	GGWCC	1 cut(s)	152
AvrII	CCTAGG	1 cut(s)	1754
BalI	TGGCCA	1 cut(s)	581
BamHI	GGATCC	1 cut(s)	212
BanI	GGYRCC	2 cut(s)	1056, 1699
BanII	GRGCYC	2 cut(s)	780, 2488
BarI	GAAGNNNNNNTAC	4 cut(s)	915, 947, 2252, 2284
BauI	CACGAG	2 cut(s)	86, 117
BbrPI	CACGTG	1 cut(s)	1805
Bbv12I	GWGCWC	3 cut(s)	87, 780, 2488
BbvI	GCAGC	9 cut(s)	560, 859, 862, 890, 1228, 1877, 2080, 2140, 2634
BccI	CCATC	9 cut(s)	571, 945, 1055, 1068, 1418, 1579, 1687, 2119, 2492
BceAI	ACGGC	3 cut(s)	768, 1298, 2727
BciT130I	CCWGG	2 cut(s)	1123, 2784
BciVI	GTATCC	1 cut(s)	2227
BclI	TGATCA	4 cut(s)	430, 681, 1138, 1720
BcnI	CCSGG	2 cut(s)	965, 2246
BcoDI	GTCTC	4 cut(s)	96, 1156, 1505, 2557
BcuI	ACTAGT	1 cut(s)	260
BfmI	CTRYAG	3 cut(s)	876, 1072, 1452
BfuAI	ACCTGC	1 cut(s)	2603
BfuI	GTATCC	1 cut(s)	2227
BglII	AGATCT	2 cut(s)	61, 904
BlnI	CCTAGG	1 cut(s)	1754
BlpI	GCTNAGC	1 cut(s)	779
Bme1390I	CCNGG	4 cut(s)	965, 1123, 2246, 2784
Bme18I	GGWCC	1 cut(s)	152
BmeT110I	CYCGRG	1 cut(s)	1270
BmgT120I	GGNCC	1 cut(s)	152
BmiI	GGNNCC	4 cut(s)	9, 214, 1058, 1701
BmrFI	CCNGG	4 cut(s)	965, 1123, 2246, 2784
BmsI	GCATC	8 cut(s)	155, 252, 517, 575, 1018, 1894, 2020, 2316
BplI	GAGNNNNNCTC	2 cut(s)	1485, 1517
BpmI	CTGGAG	3 cut(s)	501, 848, 2630
Bpu1102I	GCTNAGC	1 cut(s)	779
Bpu14I	TTCGAA	3 cut(s)	1370, 1480, 2379
BpuEI	CTTGAG	6 cut(s)	24, 482, 794, 2075, 2087, 2734
BpuMI	CCSGG	2 cut(s)	965, 2246
Bsa29I	ATCGAT	1 cut(s)	246
BsaAI	YACGTR	1 cut(s)	1805
BsaI	GGTCTC	2 cut(s)	1156, 1505
BsaJI	CCNNGG	3 cut(s)	1280, 1754, 2743
BsaWI	WCCGGW	2 cut(s)	1826, 2593
BsaXI	ACNNNNNCTCC	2 cut(s)	474, 504
Bsc4I	CCNNNNNNNGG	6 cut(s)	1053, 1066, 1086, 1295, 2498, 2600
Bse1I	ACTGG	6 cut(s)	1210, 1463, 1930, 1996, 2356, 2743
Bse3DI	GCAATG	2 cut(s)	2371, 2697
BseBI	CCWGG	2 cut(s)	1123, 2784
BseCI	ATCGAT	1 cut(s)	246
BseDI	CCNNGG	3 cut(s)	1280, 1754, 2743
BseGI	GGATG	4 cut(s)	582, 821, 1060, 2779
BseLI	CCNNNNNNNGG	6 cut(s)	1053, 1066, 1086, 1295, 2498, 2600
BseMI	GCAATG	2 cut(s)	2371, 2697
BseMII	CTCAG	8 cut(s)	660, 793, 938, 1516, 1626, 1899, 2456, 2746
BseNI	ACTGG	6 cut(s)	1210, 1463, 1930, 1996, 2356, 2743
BseRI	GAGGAG	3 cut(s)	84, 498, 1337
BseXI	GCAGC	9 cut(s)	560, 859, 862, 890, 1228, 1877, 2080, 2140, 2634
BsgI	GTGCAG	3 cut(s)	285, 650, 1623
Bsh1236I	CGCG	2 cut(s)	1473, 2509
BshFI	GGCC	3 cut(s)	581, 1288, 2742
BshNI	GGYRCC	2 cut(s)	1056, 1699
BshVI	ATCGAT	1 cut(s)	246
BsiHKAI	GWGCWC	3 cut(s)	87, 780, 2488
BsiHKCI	CYCGRG	1 cut(s)	1270
BsiSI	CCGG	4 cut(s)	965, 1827, 2246, 2594
BslFI	GGGAC	1 cut(s)	743
BslI	CCNNNNNNNGG	6 cut(s)	1053, 1066, 1086, 1295, 2498, 2600
BsmAI	GTCTC	4 cut(s)	96, 1156, 1505, 2557
BsmFI	GGGAC	1 cut(s)	743
BsnI	GGCC	3 cut(s)	581, 1288, 2742
Bso31I	GGTCTC	2 cut(s)	1156, 1505
BsoBI	CYCGRG	1 cut(s)	1270
Bsp119I	TTCGAA	3 cut(s)	1370, 1480, 2379
Bsp1286I	GDGCHC	3 cut(s)	87, 780, 2488
Bsp1407I	TGTACA	1 cut(s)	126
Bsp1720I	GCTNAGC	1 cut(s)	779
BspACI	CCGC	8 cut(s)	5, 232, 241, 638, 1044, 1285, 1948, 2509
BspANI	GGCC	3 cut(s)	581, 1288, 2742
BspCNI	CTCAG	9 cut(s)	4, 661, 792, 939, 1515, 1627, 1900, 2457, 2745
BspDI	ATCGAT	1 cut(s)	246
BspFNI	CGCG	2 cut(s)	1473, 2509
BspHI	TCATGA	1 cut(s)	1012
BspLI	GGNNCC	4 cut(s)	9, 214, 1058, 1701
BspMAI	CTGCAG	1 cut(s)	880
BspMI	ACCTGC	1 cut(s)	2603
BspPI	GGATC	8 cut(s)	207, 220, 278, 1071, 1113, 1818, 2256, 2519
BspQI	GCTCTTC	1 cut(s)	1090
BspT104I	TTCGAA	3 cut(s)	1370, 1480, 2379
BspT107I	GGYRCC	2 cut(s)	1056, 1699
BspTNI	GGTCTC	2 cut(s)	1156, 1505
BsrDI	GCAATG	2 cut(s)	2371, 2697
BsrGI	TGTACA	1 cut(s)	126
BsrI	ACTGG	6 cut(s)	1210, 1463, 1930, 1996, 2356, 2743
BssECI	CCNNGG	3 cut(s)	1280, 1754, 2743
BssSI	CACGAG	2 cut(s)	86, 117
BssT1I	CCWWGG	1 cut(s)	1754
Bst2BI	CACGAG	2 cut(s)	86, 117
Bst2UI	CCWGG	2 cut(s)	1123, 2784
Bst6I	CTCTTC	5 cut(s)	48, 102, 1090, 2178, 2258
BstAUI	TGTACA	1 cut(s)	126
BstBAI	YACGTR	1 cut(s)	1805
BstBI	TTCGAA	3 cut(s)	1370, 1480, 2379
BstC8I	GCNNGC	3 cut(s)	642, 1309, 2412
BstDSI	CCRYGG	2 cut(s)	1280, 2743
BstF5I	GGATG	4 cut(s)	582, 821, 1060, 2779
BstFNI	CGCG	2 cut(s)	1473, 2509
BstHHI	GCGC	3 cut(s)	2213, 2399, 2509
BstMAI	GTCTC	4 cut(s)	96, 1156, 1505, 2557
BstMWI	GCNNNNNNNGC	7 cut(s)	240, 628, 637, 869, 878, 952, 2720
BstNI	CCWGG	2 cut(s)	1123, 2784
BstNSI	RCATGY	2 cut(s)	133, 753
BstSCI	CCNGG	4 cut(s)	963, 1121, 2244, 2782
BstSFI	CTRYAG	3 cut(s)	876, 1072, 1452
BstUI	CGCG	2 cut(s)	1473, 2509
BstV1I	GCAGC	9 cut(s)	560, 859, 862, 890, 1228, 1877, 2080, 2140, 2634
BstX2I	RGATCY	8 cut(s)	61, 212, 904, 1076, 1118, 1823, 2248, 2511
BstYI	RGATCY	8 cut(s)	61, 212, 904, 1076, 1118, 1823, 2248, 2511
Bsu15I	ATCGAT	1 cut(s)	246
BsuI	GTATCC	1 cut(s)	2227
BsuRI	GGCC	3 cut(s)	581, 1288, 2742
BsuTUI	ATCGAT	1 cut(s)	246
BtgI	CCRYGG	2 cut(s)	1280, 2743
BtsCI	GGATG	4 cut(s)	582, 821, 1060, 2779
BtsI	GCAGTG	2 cut(s)	1943, 2014
BveI	ACCTGC	1 cut(s)	2603
Cac8I	GCNNGC	3 cut(s)	642, 1309, 2412
CaiI	CAGNNNCTG	1 cut(s)	1241
CciI	TCATGA	1 cut(s)	1012
CfoI	GCGC	3 cut(s)	2213, 2399, 2509
Cfr13I	GGNCC	1 cut(s)	152
ClaI	ATCGAT	1 cut(s)	246
Csp6I	GTAC	4 cut(s)	127, 1057, 1469, 2260
CviQI	GTAC	4 cut(s)	127, 1057, 1469, 2260
EaeI	YGGCCR	2 cut(s)	579, 2740
Eam1104I	CTCTTC	5 cut(s)	48, 102, 1090, 2178, 2258
EarI	CTCTTC	5 cut(s)	48, 102, 1090, 2178, 2258
EciI	GGCGGA	2 cut(s)	20, 1033
Ecl136II	GAGCTC	2 cut(s)	778, 2486
Eco130I	CCWWGG	1 cut(s)	1754
Eco24I	GRGCYC	2 cut(s)	780, 2488
Eco31I	GGTCTC	2 cut(s)	1156, 1505
Eco32I	GATATC	2 cut(s)	1409, 2076
Eco47I	GGWCC	1 cut(s)	152
Eco53kI	GAGCTC	2 cut(s)	778, 2486
Eco57I	CTGAAG	4 cut(s)	459, 543, 873, 2159
Eco72I	CACGTG	1 cut(s)	1805
Eco88I	CYCGRG	1 cut(s)	1270
EcoICRI	GAGCTC	2 cut(s)	778, 2486
EcoRI	GAATTC	1 cut(s)	475
EcoRII	CCWGG	2 cut(s)	1121, 2782
EcoRV	GATATC	2 cut(s)	1409, 2076
EcoT14I	CCWWGG	1 cut(s)	1754
EcoT38I	GRGCYC	2 cut(s)	780, 2488
ErhI	CCWWGG	1 cut(s)	1754
FalI	AAGNNNNNCTT	8 cut(s)	190, 222, 1084, 1116, 1087, 1119, 2115, 2147
FaqI	GGGAC	1 cut(s)	743
FauNDI	CATATG	1 cut(s)	1198
FbaI	TGATCA	4 cut(s)	430, 681, 1138, 1720
FokI	GGATG	4 cut(s)	589, 828, 1047, 2766
FriOI	GRGCYC	2 cut(s)	780, 2488
GlaI	GCGC	3 cut(s)	2212, 2398, 2508
GsuI	CTGGAG	3 cut(s)	501, 848, 2630
HaeIII	GGCC	3 cut(s)	581, 1288, 2742
HapII	CCGG	4 cut(s)	965, 1827, 2246, 2594
HhaI	GCGC	3 cut(s)	2213, 2399, 2509
Hin6I	GCGC	3 cut(s)	2211, 2397, 2507
HinP1I	GCGC	3 cut(s)	2211, 2397, 2507
HincII	GTYRAC	1 cut(s)	2683
HindII	GTYRAC	1 cut(s)	2683
HindIII	AAGCTT	3 cut(s)	345, 1032, 2636
HpaII	CCGG	4 cut(s)	965, 1827, 2246, 2594
Hpy166II	GTNNAC	7 cut(s)	76, 168, 194, 296, 455, 675, 2683
Hpy8I	GTNNAC	7 cut(s)	76, 168, 194, 296, 455, 675, 2683
Hpy99I	CGWCG	1 cut(s)	83
HpyAV	CCTTC	5 cut(s)	245, 1388, 1550, 1653, 2375
HpyCH4IV	ACGT	2 cut(s)	595, 1804
HpyF10VI	GCNNNNNNNGC	7 cut(s)	240, 628, 637, 869, 878, 952, 2720
HpySE526I	ACGT	2 cut(s)	595, 1804
HspAI	GCGC	3 cut(s)	2211, 2397, 2507
KpnI	GGTACC	1 cut(s)	1060
Ksp22I	TGATCA	4 cut(s)	430, 681, 1138, 1720
LguI	GCTCTTC	1 cut(s)	1090
LmnI	GCTCC	3 cut(s)	1878, 2430, 2457
Lsp1109I	GCAGC	9 cut(s)	560, 859, 862, 890, 1228, 1877, 2080, 2140, 2634
LweI	GCATC	8 cut(s)	155, 252, 517, 575, 1018, 1894, 2020, 2316
MaeII	ACGT	2 cut(s)	595, 1804
MflI	RGATCY	8 cut(s)	61, 212, 904, 1076, 1118, 1823, 2248, 2511
MhlI	GDGCHC	3 cut(s)	87, 780, 2488
MlsI	TGGCCA	1 cut(s)	581
MluNI	TGGCCA	1 cut(s)	581
MlyI	GAGTC	7 cut(s)	98, 1225, 1262, 1646, 1739, 1919, 2285
MmeI	TCCRAC	2 cut(s)	159, 2366
Mox20I	TGGCCA	1 cut(s)	581
MroXI	GAANNNNTTC	2 cut(s)	1099, 1982
MscI	TGGCCA	1 cut(s)	581
MseI	TTAA	5 cut(s)	558, 797, 1211, 2516, 2727
MslI	CAYNNNNRTG	2 cut(s)	1653, 1659
Msp20I	TGGCCA	1 cut(s)	581
MspA1I	CMGCKG	2 cut(s)	660, 946
MspI	CCGG	4 cut(s)	965, 1827, 2246, 2594
MspR9I	CCNGG	4 cut(s)	965, 1123, 2246, 2784
MvaI	CCWGG	2 cut(s)	1123, 2784
MvnI	CGCG	2 cut(s)	1473, 2509
MwoI	GCNNNNNNNGC	7 cut(s)	240, 628, 637, 869, 878, 952, 2720
NciI	CCSGG	2 cut(s)	965, 2246
NdeI	CATATG	1 cut(s)	1198
NlaIV	GGNNCC	4 cut(s)	9, 214, 1058, 1701
NmuCI	GTSAC	7 cut(s)	109, 256, 1534, 1638, 1800, 1877, 2537
NspI	RCATGY	2 cut(s)	133, 753
NspV	TTCGAA	3 cut(s)	1370, 1480, 2379
PagI	TCATGA	1 cut(s)	1012
PciI	ACATGT	2 cut(s)	129, 749
PciSI	GCTCTTC	1 cut(s)	1090
PcsI	WCGNNNNNNNCGW	2 cut(s)	84, 1477
PdmI	GAANNNNTTC	2 cut(s)	1099, 1982
PfeI	GAWTC	8 cut(s)	365, 634, 891, 1040, 1184, 1669, 1841, 2704
PleI	GAGTC	7 cut(s)	97, 1224, 1262, 1645, 1738, 1918, 2285
PmaCI	CACGTG	1 cut(s)	1805
PmlI	CACGTG	1 cut(s)	1805
PpsI	GAGTC	7 cut(s)	97, 1224, 1262, 1645, 1738, 1918, 2285
Ppu21I	YACGTR	1 cut(s)	1805
PscI	ACATGT	2 cut(s)	129, 749
Psp124BI	GAGCTC	2 cut(s)	780, 2488
Psp6I	CCWGG	2 cut(s)	1121, 2782
PspCI	CACGTG	1 cut(s)	1805
PspGI	CCWGG	2 cut(s)	1121, 2782
PspN4I	GGNNCC	4 cut(s)	9, 214, 1058, 1701
PspPI	GGNCC	1 cut(s)	152
PsrI	GAACNNNNNNTAC	2 cut(s)	295, 327
PstI	CTGCAG	1 cut(s)	880
PstNI	CAGNNNCTG	1 cut(s)	1241
PsuI	RGATCY	8 cut(s)	61, 212, 904, 1076, 1118, 1823, 2248, 2511
PvuII	CAGCTG	2 cut(s)	660, 946
RsaI	GTAC	4 cut(s)	128, 1058, 1470, 2261
RsaNI	GTAC	4 cut(s)	127, 1057, 1469, 2260
RseI	CAYNNNNRTG	2 cut(s)	1653, 1659
SacI	GAGCTC	2 cut(s)	780, 2488
SapI	GCTCTTC	1 cut(s)	1090
SaqAI	TTAA	5 cut(s)	558, 797, 1211, 2516, 2727
Sau96I	GGNCC	1 cut(s)	152
SchI	GAGTC	7 cut(s)	98, 1225, 1262, 1646, 1739, 1919, 2285
ScrFI	CCNGG	4 cut(s)	965, 1123, 2246, 2784
SduI	GDGCHC	3 cut(s)	87, 780, 2488
SfaNI	GCATC	8 cut(s)	155, 252, 517, 575, 1018, 1894, 2020, 2316
SfcI	CTRYAG	3 cut(s)	876, 1072, 1452
SfuI	TTCGAA	3 cut(s)	1370, 1480, 2379
SinI	GGWCC	1 cut(s)	152
SmiMI	CAYNNNNRTG	2 cut(s)	1653, 1659
SmlI	CTYRAG	6 cut(s)	39, 461, 773, 2054, 2066, 2749
SmoI	CTYRAG	6 cut(s)	39, 461, 773, 2054, 2066, 2749
SpeI	ACTAGT	1 cut(s)	260
SsiI	CCGC	8 cut(s)	5, 232, 241, 638, 1044, 1285, 1948, 2509
SspI	AATATT	1 cut(s)	1364
SstI	GAGCTC	2 cut(s)	780, 2488
StyD4I	CCNGG	4 cut(s)	963, 1121, 2244, 2782
StyI	CCWWGG	1 cut(s)	1754
TaiI	ACGT	2 cut(s)	598, 1807
TatI	WGTACW	1 cut(s)	126
TauI	GCSGC	1 cut(s)	1288
TfiI	GAWTC	8 cut(s)	365, 634, 891, 1040, 1184, 1669, 1841, 2704
Tru1I	TTAA	5 cut(s)	558, 797, 1211, 2516, 2727
Tru9I	TTAA	5 cut(s)	558, 797, 1211, 2516, 2727
TseFI	GTSAC	7 cut(s)	109, 256, 1534, 1638, 1800, 1877, 2537
TseI	GCWGC	9 cut(s)	548, 872, 875, 878, 1241, 1865, 2093, 2153, 2622
Tsp45I	GTSAC	7 cut(s)	109, 256, 1534, 1638, 1800, 1877, 2537
TspGWI	ACGGA	5 cut(s)	225, 1096, 1196, 1833, 1865
VpaK11BI	GGWCC	1 cut(s)	152
XapI	RAATTY	5 cut(s)	475, 560, 1320, 1372, 1765
XceI	RCATGY	2 cut(s)	133, 753
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2780
XmaJI	CCTAGG	1 cut(s)	1754
XmnI	GAANNNNTTC	2 cut(s)	1099, 1982

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.