Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7C

Orthogroup ID

OG0003250

Physical Location & Seq

Genomic Coordinates Reverse (-)

7790576 .. 7796337

Gene Structure 5762 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7CG103100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2649 bp

ATGTTGGGTGACATTGCGTCTGGACCGGCCTTCAAAATCAGTCAATGGCTGGAATTGGTCCGTAAACGCAGTGCCAAATACCATTCCTCTGGCTTCCCTCGCCGCCCATTAAGGCTTGACACAATGCCTGCTAGTGCAGGAGAGTGTAGTGAGGACTGGAAAGGTCCTGCGTCGCCTGAGCAAACCACAGAAGTCAATCTCTGGGAAAGACTTGGTAAAGCTGCTACATTGGACATTGAGTCGAGTTTTTTCTCATGGGATAGGCTTTCTTCACTTCACCACACTGAACATAGTAGTAGCAATGATCAGTCTGAGGATGAAATGAACAAAGCGCTGGAGGTCACCGTGAATTCGGGGGGAGTTGTTTTCTTTGCCCTTTTCAACCAGCCAGGGACTGATGATAACTCTTCTAAGGAAGCAGCGGCTGTTATAAAGATATCTTCATCAAGGATGGCCACACAGTCTGAACGTCTAGGTTACGAATTTGCAAAGTGGCTTGGAGTTCGAACTCCACAGGCCAGAGTCATACATAATTGTAGTATGGAGTGGCTCCAGATGAAGGAAGCAGCAGAAAAAGCAAGGGAAGCAGCAGTTTCAGAAGGAGATGAATGTGGGGAAATGACATGTTCAGAACTTTTGGAAGCTCTTGAACTTAGCCGATGCCTTTTGCTCATGAGCTATGTTCACGGATCTCCTTTACTTGAAAGCTCAAGTGTATTTGAGGTGAAGGAAACTGCAGAGAAAACAGCAGCTTCTCTTGGTAGGATCTTGATGTTGGACCTTGTCATCAGAAATGAAGATAGACTACCTTGCCGTCAGCTCAGATGGCGTGGAAACTTGGCAAATCTATTGTTTGCAGACAAAATGGATTTTGGAAATATGGACAGATTGGAGGAAGCATTTGATTCTGCCATCAAGAGATACAAACCAAGAGTGATCAGAGCTCTTCAAAAGGATAGAAGGGCAACTTCAGTAGATATCAGATTGCATAATACAGCATTGGTACCACAAAAATCTGATCTTTCTGACATTATGGATTCGCCAAGGTCTAGTCAGATGAGCGTAAAGAGTCAACTCTCAGATGATTTAATGTTTTCCGATTTTCCAGTTGTGGCTATTGATTCTGGCGTTCCTCGTCGACCTCCTGCTGGAAAACGTGCAAATGACCAGGAAATTTATCCAAAGTTGGTTGAGTTACTACTGAACAATTCTGAATACTCCTCTAATCTGTTACATGACATAACATTAGGGAAATTAGGACGTCCTCCGTTACAAGATACTGATGCATCTGATACACAAGCAATGGAAATGACTTCGGTTGTTCAAGGGTTCCGTAGTGGGTTCAAACGTGCTCTCAGGGATCTGCAAGGATTCCATATCTTCTTACTCACACTTCATCAAAAACTGGAGAATTTGATACGTATATTTTTCAATATTATAGATAAAATAGGGGAGTCCGATAAGGAGGATTTGGTAGTTACTGAGTCTCCACCACAAGCTGCCGGAAGTGGTTGTTTTCCTTCTATACCAAGTAAGGAGAGGCTTGTCAATGAGAACCATTCAGATTTTAGTGATTCCGAATTGCAGAGAACTGCTCCAAGGCCTTCAACTTCAGGACTTAAAGAAAGCTGTGACTGCAGCTCTCCCATGTCACGGGAGAGTTGGCACGGAAAGTTTTGGAAAGGGAGTGCAAGTTCAGATCCCCTCCGTAGTCTGCGTTTGACAGCAAAGCTCCGGGACTTTCATAAAAATGCCAAGGTTGATGCTGAAAGAAACAAAGAATTGGAACAGTGGAATGAAATGCTAAAGAGCGATGCAGTCAAACTTTGCCAGGAGAACAATTTTAATGCTGGGTTCTTTGAGGGTAGTGACAATAACGGCGTAGTTGACGCTTATGAATTGAAGGTCAGACTTGAGCACATTCTCGAGAGAATTTCATTGATATCTGAGGCTGCAAATACAGAGAGGCCATCTCGAATCACAAGTAGCCTGTTTATTGGTGGAGCGCTTGCTGCACGATCTGTATACACACTGCAACGCTTAGGAATCACTCATATATTATGTTTGTGTTCCAATGAAATTGGACAGTCAGATTCTCAGTTTCCTGATCTATTTGAGTACAAAAACTTCTCTATATGTGACAGTGAAGATTTCAACATCAGCAACATCTTTGATGAAGCTGTTGAGTTTATTGATCGTGTTGAAGAAACAGGAGGGAAGGTTCTAGTCCATTGCTTTGAGGGAAAAAGTAGAAGTGCTACACTAGTGCTTGCATACCTAATGCTCAGAAAGAATTCCACTCTATTAGAAGCATGGAGTTCTCTAAAGCAGGTTCATCGCCGAGCACAACCCAACGATGGGTTTGCGAAGATCTTGTTGAATTTGGATAAGAAACTGCATGGTAAAGTTTCAATGGAGTGGCATCAGCGGAAGCCAATGATGAAAGTTTGCCCCATCTGTGGGGTGAATGCGGGTCTGAGTAGCAGCTCTCTTAAGCTTCATTTACAGAAATCACATAAGAAACTATCGTCTGGTAGCGTGGATAGCGCAATGACAATGGAAATACAAAAGGCTTTGACAGTACTAAAAATCAGTCGAGGCGGAAGTGTCAGCCCCACACAAAGATCTCATTCAGATGTTGAAGAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000226 GO:0001101 GO:0003674 GO:0003824 GO:0004721 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007010 GO:0007017 GO:0008138 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009987 GO:0010033 GO:0010119 GO:0010468 GO:0016043 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019222 GO:0019538 GO:0030865 GO:0031122 GO:0033993 GO:0036211 GO:0042221 GO:0042578 GO:0043170 GO:0043412 GO:0043622 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0060255 GO:0065007 GO:0071704 GO:0071840 GO:0097305 GO:0097435 GO:0140096 GO:1901564 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Act-Frag_cataly DSPc

Protein Analysis

882

Amino Acids

98.6

Weight (kDa)

6.18

Isoelectric Point (pI)

52.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Act-Frag_cataly	PF09192	64 - 377	2.2e-46	Actin-fragmin kinase, catalytic
DSPc	PF00782	666 - 796	8.7e-25	Dual specificity phosphatase, catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003250)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G23720 AT5G23720 AT5G23720
fragaria_vesca	FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220
malus_domestica	MD06G1132400.v1.1 MD14G1148800.v1.1
prunus_persica	Prupe.5G143400_v2.0.a1
pyrus_communis	pycom06g12400 pycom14g12360
rosa_chinensis	RchiOBHm_Chr4g0416471 RchiOBHm_Chr7g0188941 RchiOBHm_Chr7g0194331
rosa_laevigata	RLG00000004049 RLG00000004648
rosa_multiflora	Rmu_co8071116.1_g000001 Rmu_sc0007831.1_g000001 Rmu_sc0032883.1_g000001 Rmu_ssc0000113.1_g000006
rosa_roxburghii	Rroxscaffold_3G00265920
rosa_rugosa	Rorug06G0494800 Rorug06G0494900 Rorug06G0495000
rosa_samantha	Rh7AG099700 Rh7BG101800 Rh7CG103100 Rh7CG146600 Rh7DG101600 Rh7DG144200
rosa_wichuraiana	Rw7G008590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	431
AatII	GACGTC	1 cut(s)	1264
Acc36I	ACCTGC	1 cut(s)	2320
Acc65I	GGTACC	1 cut(s)	1003
AccB1I	GGYRCC	1 cut(s)	1003
AccI	GTMKAC	2 cut(s)	1138, 2025
AciI	CCGC	5 cut(s)	103, 422, 2428, 2471, 2601
AclWI	GGATC	4 cut(s)	697, 773, 1368, 1694
AcoI	YGGCCR	1 cut(s)	453
AcsI	RAATTY	7 cut(s)	349, 482, 1173, 1411, 1932, 2293, 2380
AcuI	CTGAAG	2 cut(s)	954, 1596
AcyI	GRCGYC	1 cut(s)	1261
AfaI	GTAC	3 cut(s)	1005, 2120, 2583
AfeI	AGCGCT	2 cut(s)	333, 2007
AfiI	CCNNNNNNNGG	7 cut(s)	559, 1148, 1653, 2357, 2358, 2459, 2460
AflII	CTTAAG	1 cut(s)	2492
AflIII	ACRYGT	1 cut(s)	623
AhdI	GACNNNNNGTC	2 cut(s)	16, 238
AhlI	ACTAGT	1 cut(s)	2263
AjnI	CCWGG	3 cut(s)	388, 1167, 1830
AloI	GAACNNNNNNTCC	4 cut(s)	1678, 1710, 2205, 2237
Alw21I	GWGCWC	4 cut(s)	946, 1354, 1920, 2347
Alw26I	GTCTC	1 cut(s)	1491
AlwI	GGATC	4 cut(s)	697, 773, 1368, 1694
AlwNI	CAGNNNCTG	2 cut(s)	395, 425
Ama87I	CYCGRG	1 cut(s)	1925
Aor51HI	AGCGCT	2 cut(s)	333, 2007
AoxI	GGCC	5 cut(s)	27, 453, 516, 1601, 1967
ApoI	RAATTY	7 cut(s)	349, 482, 1173, 1411, 1932, 2293, 2380
Asp700I	GAANNNNTTC	1 cut(s)	2293
Asp718I	GGTACC	1 cut(s)	1003
AspLEI	GCGC	3 cut(s)	334, 2008, 2549
AspS9I	GGNCC	4 cut(s)	23, 58, 164, 778
AsuC2I	CCSGG	1 cut(s)	1736
AsuHPI	GGTGA	5 cut(s)	20, 269, 334, 736, 2476
AsuII	TTCGAA	1 cut(s)	505
AvaI	CYCGRG	1 cut(s)	1925
AvaII	GGWCC	4 cut(s)	23, 58, 164, 778
BalI	TGGCCA	1 cut(s)	455
BanI	GGYRCC	1 cut(s)	1003
BanII	GRGCYC	1 cut(s)	946
BarI	GAAGNNNNNNTAC	4 cut(s)	789, 821, 2111, 2143
Bbv12I	GWGCWC	4 cut(s)	946, 1354, 1920, 2347
BccI	CCATC	6 cut(s)	445, 819, 920, 1978, 2351, 2462
BceAI	ACGGC	2 cut(s)	798, 1894
BcgI	CGANNNNNNTGC	4 cut(s)	2318, 2345, 2352, 2379
BciT130I	CCWGG	3 cut(s)	390, 1169, 1832
BclI	TGATCA	2 cut(s)	304, 936
BcnI	CCSGG	1 cut(s)	1736
BcoDI	GTCTC	1 cut(s)	1491
BcuI	ACTAGT	1 cut(s)	2263
BfaI	CTAG	5 cut(s)	132, 473, 1050, 2225, 2264
BfmI	CTRYAG	2 cut(s)	735, 1636
BfoI	RGCGCY	2 cut(s)	335, 2009
BfrI	CTTAAG	1 cut(s)	2492
BfuAI	ACCTGC	1 cut(s)	2320
BglII	AGATCT	2 cut(s)	2370, 2624
BmcAI	AGTACT	1 cut(s)	2583
Bme1390I	CCNGG	4 cut(s)	390, 1169, 1736, 1832
Bme18I	GGWCC	4 cut(s)	23, 58, 164, 778
BmeRI	GACNNNNNGTC	2 cut(s)	16, 238
BmeT110I	CYCGRG	1 cut(s)	1925
BmgT120I	GGNCC	4 cut(s)	23, 58, 164, 778
BmiI	GGNNCC	3 cut(s)	551, 1005, 1331
BmrFI	CCNGG	4 cut(s)	390, 1169, 1736, 1832
BmsI	GCATC	6 cut(s)	650, 1273, 1295, 1753, 1804, 2431
BplI	GAGNNNNNCTC	4 cut(s)	1579, 1611, 1957, 1989
BpmI	CTGGAG	3 cut(s)	356, 536, 1427
Bpu10I	CCTNAGC	2 cut(s)	177, 2041
Bpu14I	TTCGAA	1 cut(s)	505
BpuEI	CTTGAG	2 cut(s)	694, 1934
BpuMI	CCSGG	1 cut(s)	1736
BsaAI	YACGTR	1 cut(s)	1421
BsaHI	GRCGYC	1 cut(s)	1261
BsaJI	CCNNGG	4 cut(s)	389, 1043, 1598, 1755
BsaXI	ACNNNNNCTCC	6 cut(s)	1471, 1501, 1678, 1708, 2205, 2235
Bsc4I	CCNNNNNNNGG	7 cut(s)	559, 1148, 1653, 2357, 2358, 2459, 2460
Bse118I	RCCGGY	1 cut(s)	25
Bse1I	ACTGG	3 cut(s)	161, 1106, 1410
Bse3DI	GCAATG	5 cut(s)	12, 307, 1308, 2230, 2556
BseBI	CCWGG	3 cut(s)	390, 1169, 1832
BseDI	CCNNGG	4 cut(s)	389, 1043, 1598, 1755
BseGI	GGATG	2 cut(s)	322, 456
BseLI	CCNNNNNNNGG	7 cut(s)	559, 1148, 1653, 2357, 2358, 2459, 2460
BseMI	GCAATG	5 cut(s)	12, 307, 1308, 2230, 2556
BseNI	ACTGG	3 cut(s)	161, 1106, 1410
BseRI	GAGGAG	1 cut(s)	1210
BseYI	CCCAGC	1 cut(s)	1850
BsgI	GTGCAG	2 cut(s)	156, 1998
BshFI	GGCC	5 cut(s)	29, 455, 518, 1603, 1969
BshNI	GGYRCC	1 cut(s)	1003
BsiHKAI	GWGCWC	4 cut(s)	946, 1354, 1920, 2347
BsiHKCI	CYCGRG	1 cut(s)	1925
BsiSI	CCGG	3 cut(s)	26, 1503, 1735
BslFI	GGGAC	2 cut(s)	406, 1751
BslI	CCNNNNNNNGG	7 cut(s)	559, 1148, 1653, 2357, 2358, 2459, 2460
BsmAI	GTCTC	1 cut(s)	1491
BsmFI	GGGAC	2 cut(s)	406, 1751
BsmI	GAATGC	1 cut(s)	2473
BsnI	GGCC	5 cut(s)	29, 455, 518, 1603, 1969
BsoBI	CYCGRG	1 cut(s)	1925
Bsp119I	TTCGAA	1 cut(s)	505
Bsp1286I	GDGCHC	4 cut(s)	946, 1354, 1920, 2347
BspACI	CCGC	5 cut(s)	103, 422, 2428, 2471, 2601
BspANI	GGCC	5 cut(s)	29, 455, 518, 1603, 1969
BspHI	TCATGA	1 cut(s)	672
BspLI	GGNNCC	3 cut(s)	551, 1005, 1331
BspMAI	CTGCAG	2 cut(s)	739, 1640
BspMI	ACCTGC	1 cut(s)	2320
BspPI	GGATC	4 cut(s)	697, 773, 1368, 1694
BspQI	GCTCTTC	1 cut(s)	951
BspT104I	TTCGAA	1 cut(s)	505
BspT107I	GGYRCC	1 cut(s)	1003
BspTI	CTTAAG	1 cut(s)	2492
BsrDI	GCAATG	5 cut(s)	12, 307, 1308, 2230, 2556
BsrFI	RCCGGY	1 cut(s)	25
BsrI	ACTGG	3 cut(s)	161, 1106, 1410
BssAI	RCCGGY	1 cut(s)	25
BssECI	CCNNGG	4 cut(s)	389, 1043, 1598, 1755
BssNAI	GTATAC	1 cut(s)	2026
BssNI	GRCGYC	1 cut(s)	1261
BssT1I	CCWWGG	3 cut(s)	1043, 1598, 1755
Bst1107I	GTATAC	1 cut(s)	2026
Bst2UI	CCWGG	3 cut(s)	390, 1169, 1832
Bst4CI	ACNGT	6 cut(s)	346, 462, 1791, 2088, 2144, 2581
Bst6I	CTCTTC	3 cut(s)	412, 951, 2637
BstACI	GRCGYC	1 cut(s)	1261
BstAFI	CTTAAG	1 cut(s)	2492
BstBAI	YACGTR	1 cut(s)	1421
BstBI	TTCGAA	1 cut(s)	505
BstC8I	GCNNGC	3 cut(s)	129, 2010, 2271
BstEII	GGTNACC	1 cut(s)	340
BstF5I	GGATG	2 cut(s)	322, 456
BstH2I	RGCGCY	2 cut(s)	335, 2009
BstHHI	GCGC	3 cut(s)	334, 2008, 2549
BstMAI	GTCTC	1 cut(s)	1491
BstMWI	GCNNNNNNNGC	7 cut(s)	99, 575, 584, 826, 1635, 2012, 2544
BstNI	CCWGG	3 cut(s)	390, 1169, 1832
BstNSI	RCATGY	1 cut(s)	627
BstPI	GGTNACC	1 cut(s)	340
BstSCI	CCNGG	4 cut(s)	388, 1167, 1734, 1830
BstSFI	CTRYAG	2 cut(s)	735, 1636
BstSNI	TACGTA	1 cut(s)	1421
BstX2I	RGATCY	6 cut(s)	689, 765, 1360, 1699, 2370, 2624
BstXI	CCANNNNNNTGG	1 cut(s)	89
BstYI	RGATCY	6 cut(s)	689, 765, 1360, 1699, 2370, 2624
BstZ17I	GTATAC	1 cut(s)	2026
BsuRI	GGCC	5 cut(s)	29, 455, 518, 1603, 1969
BtgZI	GCGATG	2 cut(s)	1827, 2321
BtsCI	GGATG	2 cut(s)	322, 456
BtsI	GCAGTG	2 cut(s)	76, 2030
BtsIMutI	CAGTG	5 cut(s)	76, 282, 1796, 2030, 2149
BveI	ACCTGC	1 cut(s)	2320
Cac8I	GCNNGC	3 cut(s)	129, 2010, 2271
CaiI	CAGNNNCTG	2 cut(s)	395, 425
CciI	TCATGA	1 cut(s)	672
CfoI	GCGC	3 cut(s)	334, 2008, 2549
Cfr10I	RCCGGY	1 cut(s)	25
Cfr13I	GGNCC	4 cut(s)	23, 58, 164, 778
CseI	GACGC	3 cut(s)	6, 159, 1898
Csp6I	GTAC	3 cut(s)	1004, 2119, 2582
CviAII	CATG	7 cut(s)	255, 624, 673, 1235, 1648, 2313, 2399
CviQI	GTAC	3 cut(s)	1004, 2119, 2582
DriI	GACNNNNNGTC	2 cut(s)	16, 238
EaeI	YGGCCR	1 cut(s)	453
Eam1104I	CTCTTC	3 cut(s)	412, 951, 2637
Eam1105I	GACNNNNNGTC	2 cut(s)	16, 238
EarI	CTCTTC	3 cut(s)	412, 951, 2637
EciI	GGCGGA	1 cut(s)	2616
Ecl136II	GAGCTC	1 cut(s)	944
Eco105I	TACGTA	1 cut(s)	1421
Eco130I	CCWWGG	3 cut(s)	1043, 1598, 1755
Eco147I	AGGCCT	1 cut(s)	1603
Eco24I	GRGCYC	1 cut(s)	946
Eco32I	GATATC	3 cut(s)	438, 979, 1944
Eco47I	GGWCC	4 cut(s)	23, 58, 164, 778
Eco47III	AGCGCT	2 cut(s)	333, 2007
Eco53kI	GAGCTC	1 cut(s)	944
Eco57I	CTGAAG	2 cut(s)	954, 1596
Eco88I	CYCGRG	1 cut(s)	1925
Eco91I	GGTNACC	1 cut(s)	340
EcoICRI	GAGCTC	1 cut(s)	944
EcoO109I	RGGNCCY	1 cut(s)	164
EcoO65I	GGTNACC	1 cut(s)	340
EcoRI	GAATTC	2 cut(s)	349, 2293
EcoRII	CCWGG	3 cut(s)	388, 1167, 1830
EcoRV	GATATC	3 cut(s)	438, 979, 1944
EcoT14I	CCWWGG	3 cut(s)	1043, 1598, 1755
EcoT22I	ATGCAT	1 cut(s)	1288
EcoT38I	GRGCYC	1 cut(s)	946
ErhI	CCWWGG	3 cut(s)	1043, 1598, 1755
FaeI	CATG	7 cut(s)	258, 627, 676, 1238, 1651, 2316, 2402
FalI	AAGNNNNNCTT	2 cut(s)	952, 984
FaqI	GGGAC	2 cut(s)	406, 1751
FatI	CATG	7 cut(s)	254, 623, 672, 1234, 1647, 2312, 2398
FauI	CCCGC	1 cut(s)	2464
FbaI	TGATCA	2 cut(s)	304, 936
FblI	GTMKAC	2 cut(s)	1138, 2025
FokI	GGATG	2 cut(s)	329, 463
FriOI	GRGCYC	1 cut(s)	946
FspBI	CTAG	5 cut(s)	132, 473, 1050, 2225, 2264
GlaI	GCGC	3 cut(s)	333, 2007, 2548
GsaI	CCCAGC	1 cut(s)	1854
GsuI	CTGGAG	3 cut(s)	356, 536, 1427
HaeII	RGCGCY	2 cut(s)	335, 2009
HaeIII	GGCC	5 cut(s)	29, 455, 518, 1603, 1969
HapII	CCGG	3 cut(s)	26, 1503, 1735
HgaI	GACGC	3 cut(s)	6, 159, 1898
HhaI	GCGC	3 cut(s)	334, 2008, 2549
Hin1I	GRCGYC	1 cut(s)	1261
Hin1II	CATG	7 cut(s)	258, 627, 676, 1238, 1651, 2316, 2402
Hin6I	GCGC	3 cut(s)	332, 2006, 2547
HinP1I	GCGC	3 cut(s)	332, 2006, 2547
HincII	GTYRAC	3 cut(s)	1073, 1139, 1888
HindII	GTYRAC	3 cut(s)	1073, 1139, 1888
HindIII	AAGCTT	1 cut(s)	2495
HpaII	CCGG	3 cut(s)	26, 1503, 1735
HphI	GGTGA	5 cut(s)	20, 269, 334, 736, 2476
Hpy166II	GTNNAC	6 cut(s)	65, 685, 1073, 1139, 1888, 2026
Hpy8I	GTNNAC	6 cut(s)	65, 685, 1073, 1139, 1888, 2026
Hpy99I	CGWCG	2 cut(s)	175, 1140
HpyAV	CCTTC	9 cut(s)	40, 553, 593, 721, 954, 1530, 1614, 1897, 2212
HpyCH4III	ACNGT	6 cut(s)	346, 462, 1791, 2088, 2144, 2581
HpyCH4IV	ACGT	5 cut(s)	469, 1156, 1261, 1348, 1420
HpyF10VI	GCNNNNNNNGC	7 cut(s)	99, 575, 584, 826, 1635, 2012, 2544
HpySE526I	ACGT	5 cut(s)	469, 1156, 1261, 1348, 1420
Hsp92I	GRCGYC	1 cut(s)	1261
Hsp92II	CATG	7 cut(s)	258, 627, 676, 1238, 1651, 2316, 2402
HspAI	GCGC	3 cut(s)	332, 2006, 2547
KpnI	GGTACC	1 cut(s)	1007
Ksp22I	TGATCA	2 cut(s)	304, 936
LguI	GCTCTTC	1 cut(s)	951
LmnI	GCTCC	4 cut(s)	555, 1600, 1737, 2003
LweI	GCATC	6 cut(s)	650, 1273, 1295, 1753, 1804, 2431
MaeI	CTAG	5 cut(s)	132, 473, 1050, 2225, 2264
MaeII	ACGT	5 cut(s)	469, 1156, 1261, 1348, 1420
MboII	GAAGA	9 cut(s)	261, 399, 432, 809, 938, 1372, 2159, 2216, 2380
MflI	RGATCY	6 cut(s)	689, 765, 1360, 1699, 2370, 2624
MhlI	GDGCHC	4 cut(s)	946, 1354, 1920, 2347
MlsI	TGGCCA	1 cut(s)	455
MluNI	TGGCCA	1 cut(s)	455
MlyI	GAGTC	5 cut(s)	248, 531, 1078, 1463, 1493
MmeI	TCCRAC	1 cut(s)	756
Mox20I	TGGCCA	1 cut(s)	455
Mph1103I	ATGCAT	1 cut(s)	1288
MroXI	GAANNNNTTC	1 cut(s)	2293
MscI	TGGCCA	1 cut(s)	455
MseI	TTAA	5 cut(s)	110, 1088, 1620, 1845, 2493
MslI	CAYNNNNRTG	2 cut(s)	1749, 2634
Msp20I	TGGCCA	1 cut(s)	455
MspA1I	CMGCKG	2 cut(s)	422, 2428
MspCI	CTTAAG	1 cut(s)	2492
MspI	CCGG	3 cut(s)	26, 1503, 1735
MspR9I	CCNGG	4 cut(s)	390, 1169, 1736, 1832
Mva1269I	GAATGC	1 cut(s)	2473
MvaI	CCWGG	3 cut(s)	390, 1169, 1832
MwoI	GCNNNNNNNGC	7 cut(s)	99, 575, 584, 826, 1635, 2012, 2544
NciI	CCSGG	1 cut(s)	1736
NlaIII	CATG	7 cut(s)	258, 627, 676, 1238, 1651, 2316, 2402
NlaIV	GGNNCC	3 cut(s)	551, 1005, 1331
NmeAIII	GCCGAG	1 cut(s)	2366
NmuCI	GTSAC	6 cut(s)	8, 340, 1631, 1650, 1868, 2138
NsiI	ATGCAT	1 cut(s)	1288
NspI	RCATGY	1 cut(s)	627
NspV	TTCGAA	1 cut(s)	505
PaeR7I	CTCGAG	1 cut(s)	1925
PagI	TCATGA	1 cut(s)	672
PceI	AGGCCT	1 cut(s)	1603
PciI	ACATGT	1 cut(s)	623
PciSI	GCTCTTC	1 cut(s)	951
PctI	GAATGC	1 cut(s)	2473
PdmI	GAANNNNTTC	1 cut(s)	2293
PfeI	GAWTC	8 cut(s)	905, 1037, 1121, 1371, 1574, 1977, 2046, 2093
PflFI	GACNNNGTC	1 cut(s)	782
PfoI	TCCNGGA	1 cut(s)	1734
PleI	GAGTC	5 cut(s)	247, 530, 1077, 1462, 1492
PpsI	GAGTC	5 cut(s)	247, 530, 1077, 1462, 1492
Ppu21I	YACGTR	1 cut(s)	1421
PpuMI	RGGWCCY	1 cut(s)	164
PscI	ACATGT	1 cut(s)	623
PsiI	TTATAA	1 cut(s)	431
Psp124BI	GAGCTC	1 cut(s)	946
Psp5II	RGGWCCY	1 cut(s)	164
Psp6I	CCWGG	3 cut(s)	388, 1167, 1830
PspEI	GGTNACC	1 cut(s)	340
PspFI	CCCAGC	1 cut(s)	1850
PspGI	CCWGG	3 cut(s)	388, 1167, 1830
PspN4I	GGNNCC	3 cut(s)	551, 1005, 1331
PspPI	GGNCC	4 cut(s)	23, 58, 164, 778
PspPPI	RGGWCCY	1 cut(s)	164
PstI	CTGCAG	2 cut(s)	739, 1640
PstNI	CAGNNNCTG	2 cut(s)	395, 425
PsuI	RGATCY	6 cut(s)	689, 765, 1360, 1699, 2370, 2624
PsyI	GACNNNGTC	1 cut(s)	782
RsaI	GTAC	3 cut(s)	1005, 2120, 2583
RsaNI	GTAC	3 cut(s)	1004, 2119, 2582
RseI	CAYNNNNRTG	2 cut(s)	1749, 2634
SacI	GAGCTC	1 cut(s)	946
SalI	GTCGAC	1 cut(s)	1137
SapI	GCTCTTC	1 cut(s)	951
SaqAI	TTAA	5 cut(s)	110, 1088, 1620, 1845, 2493
Sau96I	GGNCC	4 cut(s)	23, 58, 164, 778
ScaI	AGTACT	1 cut(s)	2583
SchI	GAGTC	5 cut(s)	248, 531, 1078, 1463, 1493
ScrFI	CCNGG	4 cut(s)	390, 1169, 1736, 1832
SduI	GDGCHC	4 cut(s)	946, 1354, 1920, 2347
SfaNI	GCATC	6 cut(s)	650, 1273, 1295, 1753, 1804, 2431
SfcI	CTRYAG	2 cut(s)	735, 1636
Sfr274I	CTCGAG	1 cut(s)	1925
SfuI	TTCGAA	1 cut(s)	505
SinI	GGWCC	4 cut(s)	23, 58, 164, 778
SlaI	CTCGAG	1 cut(s)	1925
SmiMI	CAYNNNNRTG	2 cut(s)	1749, 2634
SmlI	CTYRAG	4 cut(s)	709, 1913, 1925, 2492
SmoI	CTYRAG	4 cut(s)	709, 1913, 1925, 2492
SnaBI	TACGTA	1 cut(s)	1421
SpeI	ACTAGT	1 cut(s)	2263
SseBI	AGGCCT	1 cut(s)	1603
SsiI	CCGC	5 cut(s)	103, 422, 2428, 2471, 2601
SspI	AATATT	1 cut(s)	1435
SspMI	CTAG	5 cut(s)	132, 473, 1050, 2225, 2264
SstI	GAGCTC	1 cut(s)	946
StuI	AGGCCT	1 cut(s)	1603
StyD4I	CCNGG	4 cut(s)	388, 1167, 1734, 1830
StyI	CCWWGG	3 cut(s)	1043, 1598, 1755
TaaI	ACNGT	6 cut(s)	346, 462, 1791, 2088, 2144, 2581
TaiI	ACGT	5 cut(s)	472, 1159, 1264, 1351, 1423
TaqI	TCGA	6 cut(s)	242, 505, 1138, 1926, 1975, 2596
TatI	WGTACW	2 cut(s)	2118, 2581
TauI	GCSGC	2 cut(s)	105, 425
TfiI	GAWTC	8 cut(s)	905, 1037, 1121, 1371, 1574, 1977, 2046, 2093
Tru1I	TTAA	5 cut(s)	110, 1088, 1620, 1845, 2493
Tru9I	TTAA	5 cut(s)	110, 1088, 1620, 1845, 2493
TscAI	CASTG	5 cut(s)	76, 289, 1796, 2037, 2149
TseFI	GTSAC	6 cut(s)	8, 340, 1631, 1650, 1868, 2138
Tsp45I	GTSAC	6 cut(s)	8, 340, 1631, 1650, 1868, 2138
TspGWI	ACGGA	6 cut(s)	50, 702, 1257, 1322, 1683, 1697
TspRI	CASTG	5 cut(s)	76, 289, 1796, 2037, 2149
Tth111I	GACNNNGTC	1 cut(s)	782
Vha464I	CTTAAG	1 cut(s)	2492
VpaK11BI	GGWCC	4 cut(s)	23, 58, 164, 778
XapI	RAATTY	7 cut(s)	349, 482, 1173, 1411, 1932, 2293, 2380
XceI	RCATGY	1 cut(s)	627
XhoI	CTCGAG	1 cut(s)	1925
XmiI	GTMKAC	2 cut(s)	1138, 2025
XmnI	GAANNNNTTC	1 cut(s)	2293
XspI	CTAG	5 cut(s)	132, 473, 1050, 2225, 2264
ZraI	GACGTC	1 cut(s)	1262
ZrmI	AGTACT	1 cut(s)	2583
Zsp2I	ATGCAT	1 cut(s)	1288

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.