Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0003250

Physical Location & Seq

Genomic Coordinates Forward (+)

5317074 .. 5323193

Gene Structure 6120 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g09220.t7

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2784 bp

ATGCAATCATCAACCCTCTGCAGACATAAAACGAATTGGGTCATGCTGAAATTGCAGGAAGACAAGGATTTGGACCCTGGATCTGATGACCTCGAGGCTCCTCTGCCTCTTACCGTCACTTCCCGGGTCTTGTATATGTTGGGTGACATTGCATCTGGACCGGCCTTTAAAATCACTCAATGGCTGGAATTGGTCCGTAAACGCAGTTCCAAACACCATGCCTCTGGCTTCCCTCGCCGCCCGTTAAGAGTGGACTCAATGCCTCCTAGTGCAGGAGAGTCTAGTGAGGAATGGAAAGGCCCTGCGTCGCCTGAGCATACCACAGAAGTCAATCTCTGGGAAAGACTTGGTAAAGCTGCTACATTGGACATTGAGTCGAGTCTTTTCTCATGGGATTGGCTTTCTTCACTTCACCACACAGAACACAGTAGTAGCACTGATCAGTCTGAGGATGAAATGAACAAAGCTCTGGAGGTGATTGTGAATTCAGGGGGAGTTGTTTTCTTTGCCCTTTTCAACCAACCAGGGACGGATGATAATTCTTCTAAAGAAGCAGCGGCTTGTATAAAGATATCATCATCAAGAATGGCCACACAGTCTGAACGTCTAGGTTACGAATTTGCAAAGTGGCTTGGAGTTCGAACTCCACAGGCCAGAGTCATACATAATTGTAGTATGGAGTGGCTCCAGATGAAGGAAGCCACAGAGAAAGCAAGAGAAGCAGCAGTTTCAGAAGGAGACGAATGTGGGGAAATGACATGTTCGGAACTTTTGGAAGCTCTTGAACTTAGCCGATGCCTTTTGCTCATGAGCTATGTTCATGGATCTCCTTTACTCGAAAGCTCAAGTGTATTTGAGGCGAAGGAAACTGCAGAAAAAACAGCAGCTTCTCTTGGTAGGATTTTGTTGTTGGACCTTGTCATCAGAAATGAAGATAGACTACCTTGTCGTCAGCTCAGATGGCGTGGAAATTCTGCAAATCTATTGTTTGCAGACAAAATGGACTTTGGAAATATGGACAGATTGGAAGAAGCATTTGATTCAGCCATCAAGAGATACAAACCAAGAGTGATCAGAGCTCTTCAAAAGGATAGAAGGGCAACTTCAGTAGATATCAGATTGCATAATGCAGGATTGGCAACACAGAAATCTGATCTTTCAGACATTATGGAGTCGCCGAGGTCTAGTCAAATGAGCGTAAAGAGTCAAGTCTCAGATGATTTAATGTTTTCTGATTTTCCAGTTGTGGCTATTGATTCTGGCGTTCCTCGTCGACCTCCTGCGGGAAAACGTGCAAATGACCAGGAAATATACCCAAAGTTGGTTGAGTTACTACTGAACAATTCTGAATACTCCTCTAATCTGTTACATGAGATAACATTAGGGAAATTAGGACGTCCTCTGCTACAAGATACTGATGCATCTGATACACAAGCAATGGATATCACTTCAGTTGTTAAAGAATTCCGTAGTGGGTTCAAACGTGCTCTTAGGGATCTGCAGGGATTCCATATCTTTCTGCTCACACTTCATCAAAAACTGGACAATTTGATACGTATATTTTTCAATATTATAGATAAAGTAGGGGAGTCTGAGAAAGAGGATTTGGTAGTTACTGAGTCTCCATCACAAGCTGCTGGAACTGGTTGTTTTCCCTCTACACCATGTAAGGAGAGGCTTGTCAATGAGAACCAGTCAGATTTTAGTGATTCTGAATTGCAGAGAACTGCTACAAGGTCCTCAACTTCGGGACTTAAAGAAAACTGTGACTGCAGCTCTCCCATGTCAAGGGAGAATTGGCACGGAAAATTATGGAAAGGGAGTGCAAGTTCAGATCCCCTCCGTTGTCTGCGCTTGACAGCAAAGCTCCGGGACTTTCATAAAAATGCCAAGGTTGATGCTGAAACGAGCAAAGAATTGGAACAGTGGAATGAAATGCTAAAGAGCGATGCCGTCAAACTTTGCCAAGAGAACAACTTTAGTGCTGGCTTCTTTGAGGGTAGTGACAATAACGGCGTAGTTGATGCTTATGAATTGAAGGTCAGACTTGAGCACATTCTCGAGAGAATTTCATTGATATCAGAGGCTGCAAATACAGAGAGGCCATCTCGAATCACAAATAGCCTGTTTATTGGTGGAGCACTTGCTGCACGATCTGTGTACACACTGCAAAGCTTAGGAATCACTCATATATTATGTTTGTGTTCCAGTGAGATCGGACAGTCAGATTCTCAGTTTCCTGATCTATTCGAGTACAAAAATTTCTCCATATGTGACAGTGAAGATTTCAACATCACCAACATCTTTGATGAAGCTATTGAGTTTATTGATCATGTTGAACAAACAGGAGGGAAGGTCCTAGTCCATTGCTTCGAGGGAAAAAGTAGAAGTGCTACGCTAGTGCTTGCATACCTAATGCTCAGAAAGAATTCCACTCTGTTAGAAGCATGGAACGCTCTAAAGCTGGTTCATCGCCGAGCACAACCCAACGACGGGTTTGCAAAGATCTTGTTAGATTTGGATAAGAAACTGCATGGTAGAGCTTCAATGGAGTGGCAACAGCGGAAGCCAATGATGAAAGTTTGCCCCATCTGTGGGGTGAGTGCGGGTCTGAGTAGCAGCTCACTTAAGCTTCATCTACAGAAATCACATAAGAAACTATCCTCCGGTAGCGTGGATAGTGCAATGACAATGGAAATACAAAAGGCTTTGACAGCACTCAAAATCACTCGAGGTGGAAGTGTCAGCCCCACACAAAGATCACATTCAGATGTTGAAGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000226 GO:0001101 GO:0003674 GO:0003824 GO:0004721 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007010 GO:0007017 GO:0008138 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009987 GO:0010033 GO:0010119 GO:0010468 GO:0016043 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019222 GO:0019538 GO:0030865 GO:0031122 GO:0033993 GO:0036211 GO:0042221 GO:0042578 GO:0043170 GO:0043412 GO:0043622 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0060255 GO:0065007 GO:0071704 GO:0071840 GO:0097305 GO:0097435 GO:0140096 GO:1901564 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Act-Frag_cataly DSPc

Protein Analysis

928

Amino Acids

103.46

Weight (kDa)

5.91

Isoelectric Point (pI)

51.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Act-Frag_cataly	PF09192	167 - 480	9.6e-46	Actin-fragmin kinase, catalytic
DSPc	PF00782	769 - 865	1e-17	Dual specificity phosphatase, catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0003250)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G23720 AT5G23720 AT5G23720
fragaria_vesca	FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220
malus_domestica	MD06G1132400.v1.1 MD14G1148800.v1.1
prunus_persica	Prupe.5G143400_v2.0.a1
pyrus_communis	pycom06g12400 pycom14g12360
rosa_chinensis	RchiOBHm_Chr4g0416471 RchiOBHm_Chr7g0188941 RchiOBHm_Chr7g0194331
rosa_laevigata	RLG00000004049 RLG00000004648
rosa_multiflora	Rmu_co8071116.1_g000001 Rmu_sc0007831.1_g000001 Rmu_sc0032883.1_g000001 Rmu_ssc0000113.1_g000006
rosa_roxburghii	Rroxscaffold_3G00265920
rosa_rugosa	Rorug06G0494800 Rorug06G0494900 Rorug06G0495000
rosa_samantha	Rh7AG099700 Rh7BG101800 Rh7CG103100 Rh7CG146600 Rh7DG101600 Rh7DG144200
rosa_wichuraiana	Rw7G008590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	945
AatII	GACGTC	1 cut(s)	1399
AbsI	CCTCGAGG	1 cut(s)	92
AccI	GTMKAC	1 cut(s)	1273
AciI	CCGC	5 cut(s)	238, 557, 1283, 2563, 2606
AclWI	GGATC	4 cut(s)	88, 832, 1503, 1829
AcoI	YGGCCR	1 cut(s)	588
AcsI	RAATTY	7 cut(s)	484, 617, 970, 1463, 2067, 2260, 2428
AcuI	CTGAAG	2 cut(s)	1089, 1434
AcyI	GRCGYC	1 cut(s)	1396
AfaI	GTAC	2 cut(s)	2162, 2255
AfiI	CCNNNNNNNGG	9 cut(s)	272, 694, 1283, 1321, 1788, 2492, 2493, 2594, 2595
AflII	CTTAAG	1 cut(s)	2627
AflIII	ACRYGT	1 cut(s)	758
AhdI	GACNNNNNGTC	1 cut(s)	373
AjnI	CCWGG	3 cut(s)	76, 523, 1302
AjuI	GAANNNNNNNTTGG	2 cut(s)	1589, 1621
AloI	GAACNNNNNNTCC	2 cut(s)	1813, 1845
Alw21I	GWGCWC	5 cut(s)	1081, 1489, 2055, 2143, 2482
Alw26I	GTCTC	3 cut(s)	732, 1216, 1626
AlwI	GGATC	4 cut(s)	88, 832, 1503, 1829
AlwNI	CAGNNNCTG	1 cut(s)	2087
Ama87I	CYCGRG	4 cut(s)	92, 123, 2060, 2730
AoxI	GGCC	5 cut(s)	162, 298, 588, 651, 2102
ApeKI	GCWGC	9 cut(s)	356, 554, 722, 884, 1634, 1773, 2087, 2147, 2619
ApoI	RAATTY	7 cut(s)	484, 617, 970, 1463, 2067, 2260, 2428
Asp700I	GAANNNNTTC	1 cut(s)	2428
AspLEI	GCGC	1 cut(s)	1854
AspS9I	GGNCC	7 cut(s)	73, 158, 193, 299, 913, 1737, 2356
AsuC2I	CCSGG	3 cut(s)	124, 125, 1871
AsuHPI	GGTGA	5 cut(s)	155, 404, 487, 2287, 2611
AsuII	TTCGAA	1 cut(s)	640
AvaI	CYCGRG	4 cut(s)	92, 123, 2060, 2730
AvaII	GGWCC	6 cut(s)	73, 158, 193, 913, 1737, 2356
BalI	TGGCCA	1 cut(s)	590
BanII	GRGCYC	1 cut(s)	1081
BarI	GAAGNNNNNNTAC	2 cut(s)	924, 956
BbsI	GAAGAC	1 cut(s)	66
Bbv12I	GWGCWC	5 cut(s)	1081, 1489, 2055, 2143, 2482
BbvI	GCAGC	9 cut(s)	343, 566, 734, 896, 1621, 1785, 2074, 2134, 2631
BccI	CCATC	5 cut(s)	954, 1055, 1633, 2113, 2597
BceAI	ACGGC	2 cut(s)	1937, 2029
BcgI	CGANNNNNNTGC	2 cut(s)	2480, 2514
BciT130I	CCWGG	3 cut(s)	78, 525, 1304
BclI	TGATCA	3 cut(s)	439, 1071, 2329
BcnI	CCSGG	3 cut(s)	124, 125, 1871
BcoDI	GTCTC	3 cut(s)	732, 1216, 1626
BfaI	CTAG	6 cut(s)	267, 282, 608, 1185, 2360, 2399
BfmI	CTRYAG	5 cut(s)	19, 870, 1499, 1771, 2639
BfrI	CTTAAG	1 cut(s)	2627
BglII	AGATCT	1 cut(s)	2505
Bme1390I	CCNGG	6 cut(s)	78, 124, 125, 525, 1304, 1871
Bme18I	GGWCC	6 cut(s)	73, 158, 193, 913, 1737, 2356
BmeRI	GACNNNNNGTC	1 cut(s)	373
BmeT110I	CYCGRG	4 cut(s)	92, 123, 2060, 2730
BmgT120I	GGNCC	7 cut(s)	73, 158, 193, 299, 913, 1737, 2356
BmiI	GGNNCC	3 cut(s)	75, 99, 686
BmrFI	CCNGG	6 cut(s)	78, 124, 125, 525, 1304, 1871
BmsI	GCATC	7 cut(s)	161, 785, 1408, 1430, 1888, 1939, 2014
BpiI	GAAGAC	1 cut(s)	66
BplI	GAGNNNNNCTC	2 cut(s)	2092, 2124
BpmI	CTGGAG	2 cut(s)	491, 671
Bpu10I	CCTNAGC	2 cut(s)	312, 2176
Bpu14I	TTCGAA	1 cut(s)	640
BpuEI	CTTGAG	2 cut(s)	829, 2069
BpuMI	CCSGG	3 cut(s)	124, 125, 1871
BsaAI	YACGTR	1 cut(s)	1556
BsaHI	GRCGYC	1 cut(s)	1396
BsaJI	CCNNGG	5 cut(s)	76, 123, 524, 1178, 1890
BsaWI	WCCGGW	1 cut(s)	2666
BsaXI	ACNNNNNCTCC	6 cut(s)	1606, 1636, 1813, 1843, 2340, 2370
Bsc4I	CCNNNNNNNGG	9 cut(s)	272, 694, 1283, 1321, 1788, 2492, 2493, 2594, 2595
Bse118I	RCCGGY	1 cut(s)	160
Bse1I	ACTGG	5 cut(s)	1241, 1545, 1648, 1693, 2208
Bse3DI	GCAATG	4 cut(s)	147, 1443, 2365, 2691
BseBI	CCWGG	3 cut(s)	78, 525, 1304
BseDI	CCNNGG	5 cut(s)	76, 123, 524, 1178, 1890
BseGI	GGATG	2 cut(s)	457, 538
BseLI	CCNNNNNNNGG	9 cut(s)	272, 694, 1283, 1321, 1788, 2492, 2493, 2594, 2595
BseMI	GCAATG	4 cut(s)	147, 1443, 2365, 2691
BseMII	CTCAG	9 cut(s)	303, 438, 970, 1227, 1584, 1608, 2246, 2434, 2603
BseNI	ACTGG	5 cut(s)	1241, 1545, 1648, 1693, 2208
BseRI	GAGGAG	2 cut(s)	90, 1345
BseXI	GCAGC	9 cut(s)	343, 566, 734, 896, 1621, 1785, 2074, 2134, 2631
BsgI	GTGCAG	2 cut(s)	291, 2133
BshFI	GGCC	5 cut(s)	164, 300, 590, 653, 2104
BsiHKAI	GWGCWC	5 cut(s)	1081, 1489, 2055, 2143, 2482
BsiHKCI	CYCGRG	4 cut(s)	92, 123, 2060, 2730
BsiSI	CCGG	4 cut(s)	124, 161, 1870, 2667
BslFI	GGGAC	3 cut(s)	541, 1764, 1886
BslI	CCNNNNNNNGG	9 cut(s)	272, 694, 1283, 1321, 1788, 2492, 2493, 2594, 2595
BsmAI	GTCTC	3 cut(s)	732, 1216, 1626
BsmBI	CGTCTC	1 cut(s)	732
BsmFI	GGGAC	3 cut(s)	541, 1764, 1886
BsnI	GGCC	5 cut(s)	164, 300, 590, 653, 2104
BsoBI	CYCGRG	4 cut(s)	92, 123, 2060, 2730
Bsp119I	TTCGAA	1 cut(s)	640
Bsp1286I	GDGCHC	5 cut(s)	1081, 1489, 2055, 2143, 2482
Bsp1407I	TGTACA	1 cut(s)	2160
BspACI	CCGC	5 cut(s)	238, 557, 1283, 2563, 2606
BspANI	GGCC	5 cut(s)	164, 300, 590, 653, 2104
BspCNI	CTCAG	9 cut(s)	304, 439, 969, 1226, 1585, 1609, 2245, 2433, 2604
BspHI	TCATGA	1 cut(s)	807
BspLI	GGNNCC	3 cut(s)	75, 99, 686
BspMAI	CTGCAG	4 cut(s)	23, 874, 1503, 1775
BspPI	GGATC	4 cut(s)	88, 832, 1503, 1829
BspQI	GCTCTTC	1 cut(s)	1086
BspT104I	TTCGAA	1 cut(s)	640
BspTI	CTTAAG	1 cut(s)	2627
BsrDI	GCAATG	4 cut(s)	147, 1443, 2365, 2691
BsrFI	RCCGGY	1 cut(s)	160
BsrGI	TGTACA	1 cut(s)	2160
BsrI	ACTGG	5 cut(s)	1241, 1545, 1648, 1693, 2208
BssAI	RCCGGY	1 cut(s)	160
BssECI	CCNNGG	5 cut(s)	76, 123, 524, 1178, 1890
BssNI	GRCGYC	1 cut(s)	1396
BssT1I	CCWWGG	1 cut(s)	1890
Bst2UI	CCWGG	3 cut(s)	78, 525, 1304
Bst4CI	ACNGT	7 cut(s)	115, 428, 597, 1766, 1926, 2223, 2279
Bst6I	CTCTTC	1 cut(s)	1086
BstACI	GRCGYC	1 cut(s)	1396
BstAFI	CTTAAG	1 cut(s)	2627
BstAPI	GCANNNNNTGC	1 cut(s)	2147
BstAUI	TGTACA	1 cut(s)	2160
BstBAI	YACGTR	1 cut(s)	1556
BstBI	TTCGAA	1 cut(s)	640
BstC8I	GCNNGC	2 cut(s)	1987, 2406
BstENI	CCTNNNNNAGG	1 cut(s)	270
BstF5I	GGATG	2 cut(s)	457, 538
BstHHI	GCGC	1 cut(s)	1854
BstMAI	GTCTC	3 cut(s)	732, 1216, 1626
BstMWI	GCNNNNNNNGC	8 cut(s)	52, 234, 719, 961, 1136, 2147, 2453, 2714
BstNI	CCWGG	3 cut(s)	78, 525, 1304
BstNSI	RCATGY	1 cut(s)	762
BstSCI	CCNGG	6 cut(s)	76, 122, 123, 523, 1302, 1869
BstSFI	CTRYAG	5 cut(s)	19, 870, 1499, 1771, 2639
BstSNI	TACGTA	1 cut(s)	1556
BstV1I	GCAGC	9 cut(s)	343, 566, 734, 896, 1621, 1785, 2074, 2134, 2631
BstV2I	GAAGAC	1 cut(s)	66
BstX2I	RGATCY	5 cut(s)	80, 824, 1495, 1834, 2505
BstXI	CCANNNNNNTGG	1 cut(s)	224
BstYI	RGATCY	5 cut(s)	80, 824, 1495, 1834, 2505
BsuRI	GGCC	5 cut(s)	164, 300, 590, 653, 2104
BtgZI	GCGATG	2 cut(s)	1962, 2456
BtsCI	GGATG	2 cut(s)	457, 538
BtsI	GCAGTG	1 cut(s)	2165
BtsIMutI	CAGTG	5 cut(s)	435, 1931, 2165, 2215, 2284
Cac8I	GCNNGC	2 cut(s)	1987, 2406
CaiI	CAGNNNCTG	1 cut(s)	2087
CciI	TCATGA	1 cut(s)	807
CfoI	GCGC	1 cut(s)	1854
Cfr10I	RCCGGY	1 cut(s)	160
Cfr13I	GGNCC	7 cut(s)	73, 158, 193, 299, 913, 1737, 2356
Cfr9I	CCCGGG	1 cut(s)	123
CseI	GACGC	1 cut(s)	294
Csp6I	GTAC	2 cut(s)	2161, 2254
CviQI	GTAC	2 cut(s)	2161, 2254
DraI	TTTAAA	1 cut(s)	169
DrdI	GACNNNNNNGTC	1 cut(s)	945
DriI	GACNNNNNGTC	1 cut(s)	373
DseDI	GACNNNNNNGTC	1 cut(s)	945
EaeI	YGGCCR	1 cut(s)	588
Eam1104I	CTCTTC	1 cut(s)	1086
Eam1105I	GACNNNNNGTC	1 cut(s)	373
EarI	CTCTTC	1 cut(s)	1086
Ecl136II	GAGCTC	1 cut(s)	1079
Eco105I	TACGTA	1 cut(s)	1556
Eco130I	CCWWGG	1 cut(s)	1890
Eco24I	GRGCYC	1 cut(s)	1081
Eco32I	GATATC	4 cut(s)	573, 1114, 1444, 2079
Eco47I	GGWCC	6 cut(s)	73, 158, 193, 913, 1737, 2356
Eco53kI	GAGCTC	1 cut(s)	1079
Eco57I	CTGAAG	2 cut(s)	1089, 1434
Eco88I	CYCGRG	4 cut(s)	92, 123, 2060, 2730
EcoICRI	GAGCTC	1 cut(s)	1079
EcoNI	CCTNNNNNAGG	1 cut(s)	270
EcoO109I	RGGNCCY	3 cut(s)	299, 1737, 2356
EcoRI	GAATTC	3 cut(s)	484, 1463, 2428
EcoRII	CCWGG	3 cut(s)	76, 523, 1302
EcoRV	GATATC	4 cut(s)	573, 1114, 1444, 2079
EcoT14I	CCWWGG	1 cut(s)	1890
EcoT22I	ATGCAT	1 cut(s)	1423
EcoT38I	GRGCYC	1 cut(s)	1081
ErhI	CCWWGG	1 cut(s)	1890
Esp3I	CGTCTC	1 cut(s)	732
FalI	AAGNNNNNCTT	2 cut(s)	1087, 1119
FaqI	GGGAC	3 cut(s)	541, 1764, 1886
FauI	CCCGC	2 cut(s)	1276, 2599
FauNDI	CATATG	1 cut(s)	2270
FbaI	TGATCA	3 cut(s)	439, 1071, 2329
FblI	GTMKAC	1 cut(s)	1273
FokI	GGATG	2 cut(s)	464, 545
FriOI	GRGCYC	1 cut(s)	1081
FspBI	CTAG	6 cut(s)	267, 282, 608, 1185, 2360, 2399
GlaI	GCGC	1 cut(s)	1853
GsuI	CTGGAG	2 cut(s)	491, 671
HaeIII	GGCC	5 cut(s)	164, 300, 590, 653, 2104
HapII	CCGG	4 cut(s)	124, 161, 1870, 2667
HgaI	GACGC	1 cut(s)	294
HhaI	GCGC	1 cut(s)	1854
Hin1I	GRCGYC	1 cut(s)	1396
Hin6I	GCGC	1 cut(s)	1852
HinP1I	GCGC	1 cut(s)	1852
HincII	GTYRAC	1 cut(s)	1274
HindII	GTYRAC	1 cut(s)	1274
HindIII	AAGCTT	2 cut(s)	2173, 2630
HpaII	CCGG	4 cut(s)	124, 161, 1870, 2667
HphI	GGTGA	5 cut(s)	155, 404, 487, 2287, 2611
Hpy166II	GTNNAC	5 cut(s)	200, 253, 1274, 2161, 2163
Hpy8I	GTNNAC	5 cut(s)	200, 253, 1274, 2161, 2163
Hpy99I	CGWCG	3 cut(s)	310, 1275, 2495
HpyAV	CCTTC	6 cut(s)	688, 728, 856, 1089, 2032, 2347
HpyCH4III	ACNGT	7 cut(s)	115, 428, 597, 1766, 1926, 2223, 2279
HpyCH4IV	ACGT	5 cut(s)	604, 1291, 1396, 1483, 1555
HpyF10VI	GCNNNNNNNGC	8 cut(s)	52, 234, 719, 961, 1136, 2147, 2453, 2714
HpySE526I	ACGT	5 cut(s)	604, 1291, 1396, 1483, 1555
Hsp92I	GRCGYC	1 cut(s)	1396
HspAI	GCGC	1 cut(s)	1852
Ksp22I	TGATCA	3 cut(s)	439, 1071, 2329
LguI	GCTCTTC	1 cut(s)	1086
LmnI	GCTCC	4 cut(s)	103, 690, 1872, 2138
Lsp1109I	GCAGC	9 cut(s)	343, 566, 734, 896, 1621, 1785, 2074, 2134, 2631
LweI	GCATC	7 cut(s)	161, 785, 1408, 1430, 1888, 1939, 2014
MaeI	CTAG	6 cut(s)	267, 282, 608, 1185, 2360, 2399
MaeII	ACGT	5 cut(s)	604, 1291, 1396, 1483, 1555
MaeIII	GTNAC	9 cut(s)	115, 143, 611, 1329, 1365, 1612, 1766, 2003, 2273
MboII	GAAGA	7 cut(s)	71, 396, 534, 944, 1040, 1073, 2294
MflI	RGATCY	5 cut(s)	80, 824, 1495, 1834, 2505
MhlI	GDGCHC	5 cut(s)	1081, 1489, 2055, 2143, 2482
MlsI	TGGCCA	1 cut(s)	590
MluNI	TGGCCA	1 cut(s)	590
MlyI	GAGTC	9 cut(s)	248, 287, 383, 388, 666, 1181, 1213, 1598, 1628
MmeI	TCCRAC	1 cut(s)	891
Mox20I	TGGCCA	1 cut(s)	590
Mph1103I	ATGCAT	1 cut(s)	1423
MroXI	GAANNNNTTC	1 cut(s)	2428
MscI	TGGCCA	1 cut(s)	590
MseI	TTAA	6 cut(s)	168, 245, 1223, 1458, 1755, 2628
MslI	CAYNNNNRTG	2 cut(s)	1884, 2769
Msp20I	TGGCCA	1 cut(s)	590
MspA1I	CMGCKG	2 cut(s)	557, 2563
MspCI	CTTAAG	1 cut(s)	2627
MspI	CCGG	4 cut(s)	124, 161, 1870, 2667
MspR9I	CCNGG	6 cut(s)	78, 124, 125, 525, 1304, 1871
MvaI	CCWGG	3 cut(s)	78, 525, 1304
MwoI	GCNNNNNNNGC	8 cut(s)	52, 234, 719, 961, 1136, 2147, 2453, 2714
NciI	CCSGG	3 cut(s)	124, 125, 1871
NdeI	CATATG	1 cut(s)	2270
NlaIV	GGNNCC	3 cut(s)	75, 99, 686
NmeAIII	GCCGAG	2 cut(s)	1203, 2501
NmuCI	GTSAC	5 cut(s)	115, 143, 1766, 2003, 2273
NsiI	ATGCAT	1 cut(s)	1423
NspI	RCATGY	1 cut(s)	762
NspV	TTCGAA	1 cut(s)	640
PaeR7I	CTCGAG	3 cut(s)	92, 2060, 2730
PagI	TCATGA	1 cut(s)	807
PciI	ACATGT	1 cut(s)	758
PciSI	GCTCTTC	1 cut(s)	1086
PdmI	GAANNNNTTC	1 cut(s)	2428
PfeI	GAWTC	7 cut(s)	1040, 1256, 1506, 1709, 2112, 2181, 2228
PflFI	GACNNNGTC	1 cut(s)	917
PfoI	TCCNGGA	1 cut(s)	1869
PleI	GAGTC	9 cut(s)	248, 286, 382, 387, 665, 1180, 1212, 1597, 1627
PpsI	GAGTC	9 cut(s)	248, 286, 382, 387, 665, 1180, 1212, 1597, 1627
Ppu21I	YACGTR	1 cut(s)	1556
PpuMI	RGGWCCY	2 cut(s)	1737, 2356
PscI	ACATGT	1 cut(s)	758
Psp124BI	GAGCTC	1 cut(s)	1081
Psp5II	RGGWCCY	2 cut(s)	1737, 2356
Psp6I	CCWGG	3 cut(s)	76, 523, 1302
PspGI	CCWGG	3 cut(s)	76, 523, 1302
PspN4I	GGNNCC	3 cut(s)	75, 99, 686
PspPI	GGNCC	7 cut(s)	73, 158, 193, 299, 913, 1737, 2356
PspPPI	RGGWCCY	2 cut(s)	1737, 2356
PspXI	VCTCGAGB	2 cut(s)	92, 2730
PsrI	GAACNNNNNNTAC	2 cut(s)	190, 222
PstI	CTGCAG	4 cut(s)	23, 874, 1503, 1775
PstNI	CAGNNNCTG	1 cut(s)	2087
PsuI	RGATCY	5 cut(s)	80, 824, 1495, 1834, 2505
PsyI	GACNNNGTC	1 cut(s)	917
RsaI	GTAC	2 cut(s)	2162, 2255
RsaNI	GTAC	2 cut(s)	2161, 2254
RseI	CAYNNNNRTG	2 cut(s)	1884, 2769
SacI	GAGCTC	1 cut(s)	1081
SalI	GTCGAC	1 cut(s)	1272
SapI	GCTCTTC	1 cut(s)	1086
SaqAI	TTAA	6 cut(s)	168, 245, 1223, 1458, 1755, 2628
Sau96I	GGNCC	7 cut(s)	73, 158, 193, 299, 913, 1737, 2356
SchI	GAGTC	9 cut(s)	248, 287, 383, 388, 666, 1181, 1213, 1598, 1628
ScrFI	CCNGG	6 cut(s)	78, 124, 125, 525, 1304, 1871
SduI	GDGCHC	5 cut(s)	1081, 1489, 2055, 2143, 2482
SfaNI	GCATC	7 cut(s)	161, 785, 1408, 1430, 1888, 1939, 2014
SfcI	CTRYAG	5 cut(s)	19, 870, 1499, 1771, 2639
Sfr274I	CTCGAG	3 cut(s)	92, 2060, 2730
SfuI	TTCGAA	1 cut(s)	640
SinI	GGWCC	6 cut(s)	73, 158, 193, 913, 1737, 2356
SlaI	CTCGAG	3 cut(s)	92, 2060, 2730
SmaI	CCCGGG	1 cut(s)	125
SmiMI	CAYNNNNRTG	2 cut(s)	1884, 2769
SmlI	CTYRAG	6 cut(s)	92, 844, 2048, 2060, 2627, 2730
SmoI	CTYRAG	6 cut(s)	92, 844, 2048, 2060, 2627, 2730
SnaBI	TACGTA	1 cut(s)	1556
SsiI	CCGC	5 cut(s)	238, 557, 1283, 2563, 2606
SspI	AATATT	1 cut(s)	1570
SspMI	CTAG	6 cut(s)	267, 282, 608, 1185, 2360, 2399
SstI	GAGCTC	1 cut(s)	1081
StyD4I	CCNGG	6 cut(s)	76, 122, 123, 523, 1302, 1869
StyI	CCWWGG	1 cut(s)	1890
TaaI	ACNGT	7 cut(s)	115, 428, 597, 1766, 1926, 2223, 2279
TaiI	ACGT	5 cut(s)	607, 1294, 1399, 1486, 1558
TatI	WGTACW	2 cut(s)	2160, 2253
TauI	GCSGC	2 cut(s)	240, 560
TfiI	GAWTC	7 cut(s)	1040, 1256, 1506, 1709, 2112, 2181, 2228
Tru1I	TTAA	6 cut(s)	168, 245, 1223, 1458, 1755, 2628
Tru9I	TTAA	6 cut(s)	168, 245, 1223, 1458, 1755, 2628
TscAI	CASTG	5 cut(s)	442, 1931, 2172, 2215, 2284
TseFI	GTSAC	5 cut(s)	115, 143, 1766, 2003, 2273
TseI	GCWGC	9 cut(s)	356, 554, 722, 884, 1634, 1773, 2087, 2147, 2619
Tsp45I	GTSAC	5 cut(s)	115, 143, 1766, 2003, 2273
TspGWI	ACGGA	5 cut(s)	185, 545, 1457, 1818, 1832
TspMI	CCCGGG	1 cut(s)	123
TspRI	CASTG	5 cut(s)	442, 1931, 2172, 2215, 2284
Tth111I	GACNNNGTC	1 cut(s)	917
Vha464I	CTTAAG	1 cut(s)	2627
VpaK11BI	GGWCC	6 cut(s)	73, 158, 193, 913, 1737, 2356
XagI	CCTNNNNNAGG	1 cut(s)	270
XapI	RAATTY	7 cut(s)	484, 617, 970, 1463, 2067, 2260, 2428
XceI	RCATGY	1 cut(s)	762
XhoI	CTCGAG	3 cut(s)	92, 2060, 2730
XmaI	CCCGGG	1 cut(s)	123
XmiI	GTMKAC	1 cut(s)	1273
XmnI	GAANNNNTTC	1 cut(s)	2428
XspI	CTAG	6 cut(s)	267, 282, 608, 1185, 2360, 2399
ZraI	GACGTC	1 cut(s)	1397
Zsp2I	ATGCAT	1 cut(s)	1423

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.