Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0003250

Physical Location & Seq

Genomic Coordinates Forward (+)

61099436 .. 61104504

Gene Structure 5069 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000004648

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2796 bp

ATGGCAAAACAGGAGCAAAAGCAGGAGCAGCCTGCAATCATCAACCCTCTGCAGGTCCAGGAAAAACAGGAAGAAGGGGATCTGGACCCTGGATCTGACGACCTCGAGGCTCCTCTGCCTCTCACCGTCACTTCCCGAGTCTTGTATATGTTGGGTGACATTGCATCTGGACCGGCCTTCAAAATCACTCAATGGCTGGAATTGGTCCGTAAACGCAGTGCCAAATACCATTCCTCTGGCTTCCCTCGCCGCCCATTAAGGCTTGACACAATGCCTGCTAGTGCAGGAGAGTGTAGTGAGGACTGGAAAGGTCCTGCGTCGCCTGAGCAAATCACAGAAGCCAATCTCTGGGAAAGACTTGGTAAAGCTGCTACATTGGACATTGAGTCAAGTCTTTTCTCATGGGATAGGCTTTCTTCACTTCACCACACTGAACATAGTAGTAGCAATGATCAGTCTGAGGATGAAATGAACAAAGCGCTGGAGGTCACCGTGAATTCGGGGGGAGTTGTTTTCTTTGCCCTTTTCAACCAGCCAGGGACTGATGATAACTCTTCTAAGGAAGCAGCAGCTGTTATAAAGATATCCTCATCGAGGATGGCCACACAGTCTGAACGTCTAGGTTACGAATTTGCAAAGTGGCTTGGAGTTCGAACACCACAGGCCAGAGTCATACATAATTGTAGTATGGAGTGGCTCCAGATGAAGGAAGCAGCAGAAAAAGCAAGGGAAGCAGCAGTTTCAGAAGGAGATGAATGTGGGGAAATGACATGTTCGGAACTTTTGGAAGCTCTTGAACTTAGCCGATGCCTTTTGCTCATGAGCTATGTTCACGGATCTCCTTTACTTGAAAGCTCAAGTGTATTTGAGGTGAAGGAAACTGCAGAGAAAACAGCAGCTTCTCTTGGTAGGATCTTGATGTTGGACCTTGTCATCAGAAATGAAGATAGACTACCTTGCCGTCAGCTCAGATGGCGTGGAAACTCGGCAAATCTGTTGTTTGCAGACAAAATGGATTTTGGAAATATGGACAGATTGGAGGAAGCATTTGATTCTGCCATCAAGAGATACAAACCAAGAGTGATCAGAGCTCTTCAAAAGGATAGAAGGGCAACTTCAGTAGATATCAGATTGCATAATACAGCATTGGTACCACAGAAATCTGATCTTTCTGACATTATGGATTCGCCAAGGTCTAGTCAGATGAGTGTAAAAAGTCAACTCTCAGATGATTTAATGTTTTCCGATTTTCCAGTTGTGGCTATTGATTCTGGCGTTCCTCGTCGACCTCCTGCTGGAAAACGTGCAAATGACCAGGAAATTTATCCAAAGTTGGTTGAGTTACTACTGAACAATTCTGAATACTCCTCTAATCTGTTACATGACATAACATTAGGGAAATTAGGACGTCCTCCGTTACAAGATACTGATGCATCTGATACACAAGCAATGGAAATGACTTCGGTTGTTCAAGGGTTCCGTAGTGGGTTCAAGCGTGCTCTCAGGGATCTGCAGGGATTCCATATCTTCTTACTCACACTTCATCAAAAACTGGAGAATTTGATACGTATATTTTTCAATATTATAGATAAAATAGGGGAGTCTGATAAAGAGGATTTGGTAGTTACTGAGTCTCCATCACAAGCTGCCGGAAGTGGTTATTTTCCTTCTACACCAAGTAAGGAGAGGCTTGTCAATGAGAACCATTCAGATTTTAGTGATTCTGAATTGCAGAGAACTGCTCCAAGGCCTTCAACTTCAGGACTTAAAGAAAGCTGTGACTGCAGCTCTCCCATGTCACGGGAGAGTTGGCACGGAAAGTTTTGGAAAGGGAGTGCAAGTTCAGATCCCCTCCGTAGTCTGCGCTTGACAGCAAAGCTCCGGGACTTTCATAAAAATGCCAAGGTCGATGCTGAAACGAACAAAGAATTGGAACAGTGGAATGAAATGCTAAAGAGCGATGCAGTCAAACTTTGCCAGGAGAACAATTTTAATGCTGGGTTCTTTGAGGGTAGTGACAATAACGGCGTAGTTGACGCTTATGAATTGAAGGTCAGACTTGAGCACATTCTCGAGAGAATTTCATTGATATCTGAGGCTGCAAATACAGAGAGGCCATCTCGAATCACAAGTAGCCTGTTTATTGGTGGAGCGCTTGCTGCACGATCTGTATACACACTGCAACGCTTAGGAATCACTCATATATTATGTTTGTGTTCCAATGAAATTGGACAGTCAGATTCTCAGTTTCCTGATCTATTTGAGTACAAAAACTTCTCTATATGTGACAGTGAAGATTTCAACATCAGCAACATCTTTGATGAAGCTGTTGAGTTTATTGATCGTGTTGAAGAAACAGGAGGGAAGGTTCTAGTCCATTGCTTTGAGGGAAAAAGTAGAAGTGCTACACTAGTGCTTGCATACCTAATGCTCAGAAAGAATTCCACTCTATTAGAAGCATGGAATTCTCTAAAGCAGGTTCATCGCCGAGCACAACCCAACGATGGGTTTGCGAAGATCTTGTTGAATTTGGATAAGAGACTGCATGGTAAAGTTTCAATGGAGTGGCATCAGCGGAAGCCAATGATGAAAGTTTGCCCCATCTGTGGGGTGAATGCGGGTCTGAGTAGCAGCTCTCTTAAGCTTCATTTACAGAAATCACATAAGAAACTATCATCTGGTAGCGTGGATAGCGCAATGACAATGGAAATACAAAAGGCTTTGACAGTACTAAAAATCAGTCGAGGTGGAAGTGTCAGCCCCACACAAAGATCTCATTCAGATGTTGAAGAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000226 GO:0001101 GO:0003674 GO:0003824 GO:0004721 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007010 GO:0007017 GO:0008138 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009987 GO:0010033 GO:0010119 GO:0010468 GO:0016043 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019222 GO:0019538 GO:0030865 GO:0031122 GO:0033993 GO:0036211 GO:0042221 GO:0042578 GO:0043170 GO:0043412 GO:0043622 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0060255 GO:0065007 GO:0071704 GO:0071840 GO:0097305 GO:0097435 GO:0140096 GO:1901564 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Act-Frag_cataly DSPc

Protein Analysis

932

Amino Acids

104.05

Weight (kDa)

5.82

Isoelectric Point (pI)

54.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Act-Frag_cataly	PF09192	113 - 426	1.1e-46	Actin-fragmin kinase, catalytic
DSPc	PF00782	715 - 844	1.4e-24	Dual specificity phosphatase, catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0003250)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G23720 AT5G23720 AT5G23720
fragaria_vesca	FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220
malus_domestica	MD06G1132400.v1.1 MD14G1148800.v1.1
prunus_persica	Prupe.5G143400_v2.0.a1
pyrus_communis	pycom06g12400 pycom14g12360
rosa_chinensis	RchiOBHm_Chr4g0416471 RchiOBHm_Chr7g0188941 RchiOBHm_Chr7g0194331
rosa_laevigata	RLG00000004049 RLG00000004648
rosa_multiflora	Rmu_co8071116.1_g000001 Rmu_sc0007831.1_g000001 Rmu_sc0032883.1_g000001 Rmu_ssc0000113.1_g000006
rosa_roxburghii	Rroxscaffold_3G00265920
rosa_rugosa	Rorug06G0494800 Rorug06G0494900 Rorug06G0495000
rosa_samantha	Rh7AG099700 Rh7BG101800 Rh7CG103100 Rh7CG146600 Rh7DG101600 Rh7DG144200
rosa_wichuraiana	Rw7G008590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	578
AatII	GACGTC	1 cut(s)	1411
AbsI	CCTCGAGG	1 cut(s)	104
Acc36I	ACCTGC	2 cut(s)	43, 2467
Acc65I	GGTACC	1 cut(s)	1150
AccB1I	GGYRCC	1 cut(s)	1150
AccB7I	CCANNNNNTGG	1 cut(s)	348
AccI	GTMKAC	2 cut(s)	1285, 2172
AciI	CCGC	3 cut(s)	250, 2575, 2618
AclWI	GGATC	6 cut(s)	87, 100, 844, 920, 1515, 1841
AcoI	YGGCCR	1 cut(s)	600
AcsI	RAATTY	8 cut(s)	496, 629, 1320, 1558, 2079, 2440, 2464, 2527
AcuI	CTGAAG	2 cut(s)	1101, 1743
AcyI	GRCGYC	1 cut(s)	1408
AfaI	GTAC	3 cut(s)	1152, 2267, 2730
AfeI	AGCGCT	2 cut(s)	480, 2154
AflII	CTTAAG	1 cut(s)	2639
AflIII	ACRYGT	1 cut(s)	770
AhdI	GACNNNNNGTC	1 cut(s)	385
AhlI	ACTAGT	1 cut(s)	2410
AjnI	CCWGG	5 cut(s)	57, 88, 535, 1314, 1977
AjuI	GAANNNNNNNTTGG	2 cut(s)	1913, 1945
AloI	GAACNNNNNNTCC	4 cut(s)	1825, 1857, 2352, 2384
Alw21I	GWGCWC	4 cut(s)	1093, 1501, 2067, 2494
Alw26I	GTCTC	2 cut(s)	1638, 2533
AlwI	GGATC	6 cut(s)	87, 100, 844, 920, 1515, 1841
AlwNI	CAGNNNCTG	2 cut(s)	542, 572
Ama87I	CYCGRG	3 cut(s)	104, 135, 2072
Aor51HI	AGCGCT	2 cut(s)	480, 2154
AoxI	GGCC	5 cut(s)	174, 600, 663, 1748, 2114
ApoI	RAATTY	8 cut(s)	496, 629, 1320, 1558, 2079, 2440, 2464, 2527
Asp700I	GAANNNNTTC	1 cut(s)	2440
Asp718I	GGTACC	1 cut(s)	1150
AspLEI	GCGC	4 cut(s)	481, 1866, 2155, 2696
AspS9I	GGNCC	6 cut(s)	55, 85, 170, 205, 311, 925
AsuC2I	CCSGG	1 cut(s)	1883
AsuHPI	GGTGA	6 cut(s)	115, 167, 416, 481, 883, 2623
AsuII	TTCGAA	1 cut(s)	652
AvaI	CYCGRG	3 cut(s)	104, 135, 2072
AvaII	GGWCC	6 cut(s)	55, 85, 170, 205, 311, 925
BalI	TGGCCA	1 cut(s)	602
BanI	GGYRCC	1 cut(s)	1150
BanII	GRGCYC	1 cut(s)	1093
BarI	GAAGNNNNNNTAC	4 cut(s)	936, 968, 2258, 2290
Bbv12I	GWGCWC	4 cut(s)	1093, 1501, 2067, 2494
BccI	CCATC	7 cut(s)	592, 966, 1067, 1645, 2125, 2498, 2609
BceAI	ACGGC	2 cut(s)	945, 2041
BcgI	CGANNNNNNTGC	4 cut(s)	2465, 2492, 2499, 2526
BciT130I	CCWGG	5 cut(s)	59, 90, 537, 1316, 1979
BclI	TGATCA	2 cut(s)	451, 1083
BcnI	CCSGG	1 cut(s)	1883
BcoDI	GTCTC	2 cut(s)	1638, 2533
BcuI	ACTAGT	1 cut(s)	2410
BfaI	CTAG	5 cut(s)	279, 620, 1197, 2372, 2411
BfmI	CTRYAG	4 cut(s)	50, 882, 1511, 1783
BfoI	RGCGCY	2 cut(s)	482, 2156
BfrI	CTTAAG	1 cut(s)	2639
BfuAI	ACCTGC	2 cut(s)	43, 2467
BglII	AGATCT	2 cut(s)	2517, 2771
BmcAI	AGTACT	1 cut(s)	2730
Bme1390I	CCNGG	6 cut(s)	59, 90, 537, 1316, 1883, 1979
Bme18I	GGWCC	6 cut(s)	55, 85, 170, 205, 311, 925
BmeRI	GACNNNNNGTC	1 cut(s)	385
BmeT110I	CYCGRG	3 cut(s)	104, 135, 2072
BmgT120I	GGNCC	6 cut(s)	55, 85, 170, 205, 311, 925
BmiI	GGNNCC	5 cut(s)	87, 111, 698, 1152, 1478
BmrFI	CCNGG	6 cut(s)	59, 90, 537, 1316, 1883, 1979
BmsI	GCATC	7 cut(s)	173, 797, 1420, 1442, 1900, 1951, 2578
BplI	GAGNNNNNCTC	4 cut(s)	1726, 1758, 2104, 2136
BpmI	CTGGAG	3 cut(s)	503, 683, 1574
Bpu10I	CCTNAGC	2 cut(s)	324, 2188
Bpu14I	TTCGAA	1 cut(s)	652
BpuEI	CTTGAG	2 cut(s)	841, 2081
BpuMI	CCSGG	1 cut(s)	1883
BsaAI	YACGTR	1 cut(s)	1568
BsaHI	GRCGYC	1 cut(s)	1408
BsaJI	CCNNGG	5 cut(s)	88, 536, 1190, 1745, 1902
BsaXI	ACNNNNNCTCC	6 cut(s)	1618, 1648, 1825, 1855, 2352, 2382
Bse118I	RCCGGY	1 cut(s)	172
Bse1I	ACTGG	3 cut(s)	308, 1253, 1557
Bse3DI	GCAATG	5 cut(s)	159, 454, 1455, 2377, 2703
BseBI	CCWGG	5 cut(s)	59, 90, 537, 1316, 1979
BseDI	CCNNGG	5 cut(s)	88, 536, 1190, 1745, 1902
BseGI	GGATG	2 cut(s)	469, 603
BseMI	GCAATG	5 cut(s)	159, 454, 1455, 2377, 2703
BseNI	ACTGG	3 cut(s)	308, 1253, 1557
BseRI	GAGGAG	2 cut(s)	102, 1357
BseYI	CCCAGC	1 cut(s)	1997
BsgI	GTGCAG	2 cut(s)	303, 2145
BshFI	GGCC	5 cut(s)	176, 602, 665, 1750, 2116
BshNI	GGYRCC	1 cut(s)	1150
BsiHKAI	GWGCWC	4 cut(s)	1093, 1501, 2067, 2494
BsiHKCI	CYCGRG	3 cut(s)	104, 135, 2072
BsiSI	CCGG	3 cut(s)	173, 1650, 1882
BslFI	GGGAC	2 cut(s)	553, 1898
BsmAI	GTCTC	2 cut(s)	1638, 2533
BsmFI	GGGAC	2 cut(s)	553, 1898
BsmI	GAATGC	1 cut(s)	2620
BsnI	GGCC	5 cut(s)	176, 602, 665, 1750, 2116
BsoBI	CYCGRG	3 cut(s)	104, 135, 2072
Bsp119I	TTCGAA	1 cut(s)	652
Bsp1286I	GDGCHC	4 cut(s)	1093, 1501, 2067, 2494
BspACI	CCGC	3 cut(s)	250, 2575, 2618
BspANI	GGCC	5 cut(s)	176, 602, 665, 1750, 2116
BspHI	TCATGA	1 cut(s)	819
BspLI	GGNNCC	5 cut(s)	87, 111, 698, 1152, 1478
BspMAI	CTGCAG	4 cut(s)	54, 886, 1515, 1787
BspMI	ACCTGC	2 cut(s)	43, 2467
BspPI	GGATC	6 cut(s)	87, 100, 844, 920, 1515, 1841
BspQI	GCTCTTC	1 cut(s)	1098
BspT104I	TTCGAA	1 cut(s)	652
BspT107I	GGYRCC	1 cut(s)	1150
BspTI	CTTAAG	1 cut(s)	2639
BsrDI	GCAATG	5 cut(s)	159, 454, 1455, 2377, 2703
BsrFI	RCCGGY	1 cut(s)	172
BsrI	ACTGG	3 cut(s)	308, 1253, 1557
BssAI	RCCGGY	1 cut(s)	172
BssECI	CCNNGG	5 cut(s)	88, 536, 1190, 1745, 1902
BssNAI	GTATAC	1 cut(s)	2173
BssNI	GRCGYC	1 cut(s)	1408
BssT1I	CCWWGG	3 cut(s)	1190, 1745, 1902
Bst1107I	GTATAC	1 cut(s)	2173
Bst2UI	CCWGG	5 cut(s)	59, 90, 537, 1316, 1979
Bst4CI	ACNGT	7 cut(s)	127, 493, 609, 1938, 2235, 2291, 2728
Bst6I	CTCTTC	3 cut(s)	559, 1098, 2784
BstACI	GRCGYC	1 cut(s)	1408
BstAFI	CTTAAG	1 cut(s)	2639
BstBAI	YACGTR	1 cut(s)	1568
BstBI	TTCGAA	1 cut(s)	652
BstC8I	GCNNGC	5 cut(s)	33, 276, 1497, 2157, 2418
BstEII	GGTNACC	1 cut(s)	487
BstENI	CCTNNNNNAGG	1 cut(s)	592
BstF5I	GGATG	2 cut(s)	469, 603
BstH2I	RGCGCY	2 cut(s)	482, 2156
BstHHI	GCGC	4 cut(s)	481, 1866, 2155, 2696
BstMAI	GTCTC	2 cut(s)	1638, 2533
BstMWI	GCNNNNNNNGC	8 cut(s)	28, 246, 722, 731, 973, 1782, 2159, 2691
BstNI	CCWGG	5 cut(s)	59, 90, 537, 1316, 1979
BstNSI	RCATGY	1 cut(s)	774
BstPI	GGTNACC	1 cut(s)	487
BstSCI	CCNGG	6 cut(s)	57, 88, 535, 1314, 1881, 1977
BstSFI	CTRYAG	4 cut(s)	50, 882, 1511, 1783
BstSNI	TACGTA	1 cut(s)	1568
BstX2I	RGATCY	8 cut(s)	79, 92, 836, 912, 1507, 1846, 2517, 2771
BstXI	CCANNNNNNTGG	1 cut(s)	236
BstYI	RGATCY	8 cut(s)	79, 92, 836, 912, 1507, 1846, 2517, 2771
BstZ17I	GTATAC	1 cut(s)	2173
BsuRI	GGCC	5 cut(s)	176, 602, 665, 1750, 2116
BtgZI	GCGATG	2 cut(s)	1974, 2468
BtsCI	GGATG	2 cut(s)	469, 603
BtsI	GCAGTG	2 cut(s)	223, 2177
BtsIMutI	CAGTG	5 cut(s)	223, 429, 1943, 2177, 2296
BveI	ACCTGC	2 cut(s)	43, 2467
Cac8I	GCNNGC	5 cut(s)	33, 276, 1497, 2157, 2418
CaiI	CAGNNNCTG	2 cut(s)	542, 572
CciI	TCATGA	1 cut(s)	819
CfoI	GCGC	4 cut(s)	481, 1866, 2155, 2696
Cfr10I	RCCGGY	1 cut(s)	172
Cfr13I	GGNCC	6 cut(s)	55, 85, 170, 205, 311, 925
CseI	GACGC	2 cut(s)	306, 2045
Csp6I	GTAC	3 cut(s)	1151, 2266, 2729
CviAII	CATG	7 cut(s)	402, 771, 820, 1382, 1795, 2460, 2546
CviQI	GTAC	3 cut(s)	1151, 2266, 2729
DriI	GACNNNNNGTC	1 cut(s)	385
EaeI	YGGCCR	1 cut(s)	600
Eam1104I	CTCTTC	3 cut(s)	559, 1098, 2784
Eam1105I	GACNNNNNGTC	1 cut(s)	385
EarI	CTCTTC	3 cut(s)	559, 1098, 2784
Ecl136II	GAGCTC	1 cut(s)	1091
Eco105I	TACGTA	1 cut(s)	1568
Eco130I	CCWWGG	3 cut(s)	1190, 1745, 1902
Eco147I	AGGCCT	1 cut(s)	1750
Eco24I	GRGCYC	1 cut(s)	1093
Eco32I	GATATC	3 cut(s)	585, 1126, 2091
Eco47I	GGWCC	6 cut(s)	55, 85, 170, 205, 311, 925
Eco47III	AGCGCT	2 cut(s)	480, 2154
Eco53kI	GAGCTC	1 cut(s)	1091
Eco57I	CTGAAG	2 cut(s)	1101, 1743
Eco88I	CYCGRG	3 cut(s)	104, 135, 2072
Eco91I	GGTNACC	1 cut(s)	487
EcoICRI	GAGCTC	1 cut(s)	1091
EcoNI	CCTNNNNNAGG	1 cut(s)	592
EcoO109I	RGGNCCY	1 cut(s)	311
EcoO65I	GGTNACC	1 cut(s)	487
EcoRI	GAATTC	3 cut(s)	496, 2440, 2464
EcoRII	CCWGG	5 cut(s)	57, 88, 535, 1314, 1977
EcoRV	GATATC	3 cut(s)	585, 1126, 2091
EcoT14I	CCWWGG	3 cut(s)	1190, 1745, 1902
EcoT22I	ATGCAT	1 cut(s)	1435
EcoT38I	GRGCYC	1 cut(s)	1093
ErhI	CCWWGG	3 cut(s)	1190, 1745, 1902
FaeI	CATG	7 cut(s)	405, 774, 823, 1385, 1798, 2463, 2549
FalI	AAGNNNNNCTT	2 cut(s)	1099, 1131
FaqI	GGGAC	2 cut(s)	553, 1898
FatI	CATG	7 cut(s)	401, 770, 819, 1381, 1794, 2459, 2545
FauI	CCCGC	1 cut(s)	2611
FbaI	TGATCA	2 cut(s)	451, 1083
FblI	GTMKAC	2 cut(s)	1285, 2172
FokI	GGATG	2 cut(s)	476, 610
FriOI	GRGCYC	1 cut(s)	1093
FspBI	CTAG	5 cut(s)	279, 620, 1197, 2372, 2411
GlaI	GCGC	4 cut(s)	480, 1865, 2154, 2695
GsaI	CCCAGC	1 cut(s)	2001
GsuI	CTGGAG	3 cut(s)	503, 683, 1574
HaeII	RGCGCY	2 cut(s)	482, 2156
HaeIII	GGCC	5 cut(s)	176, 602, 665, 1750, 2116
HapII	CCGG	3 cut(s)	173, 1650, 1882
HgaI	GACGC	2 cut(s)	306, 2045
HhaI	GCGC	4 cut(s)	481, 1866, 2155, 2696
Hin1I	GRCGYC	1 cut(s)	1408
Hin1II	CATG	7 cut(s)	405, 774, 823, 1385, 1798, 2463, 2549
Hin6I	GCGC	4 cut(s)	479, 1864, 2153, 2694
HinP1I	GCGC	4 cut(s)	479, 1864, 2153, 2694
HincII	GTYRAC	3 cut(s)	1220, 1286, 2035
HindII	GTYRAC	3 cut(s)	1220, 1286, 2035
HindIII	AAGCTT	1 cut(s)	2642
HpaII	CCGG	3 cut(s)	173, 1650, 1882
HphI	GGTGA	6 cut(s)	115, 167, 416, 481, 883, 2623
Hpy166II	GTNNAC	6 cut(s)	212, 832, 1220, 1286, 2035, 2173
Hpy8I	GTNNAC	6 cut(s)	212, 832, 1220, 1286, 2035, 2173
Hpy99I	CGWCG	2 cut(s)	322, 1287
HpyCH4III	ACNGT	7 cut(s)	127, 493, 609, 1938, 2235, 2291, 2728
HpyCH4IV	ACGT	4 cut(s)	616, 1303, 1408, 1567
HpyF10VI	GCNNNNNNNGC	8 cut(s)	28, 246, 722, 731, 973, 1782, 2159, 2691
HpySE526I	ACGT	4 cut(s)	616, 1303, 1408, 1567
Hsp92I	GRCGYC	1 cut(s)	1408
Hsp92II	CATG	7 cut(s)	405, 774, 823, 1385, 1798, 2463, 2549
HspAI	GCGC	4 cut(s)	479, 1864, 2153, 2694
KpnI	GGTACC	1 cut(s)	1154
Ksp22I	TGATCA	2 cut(s)	451, 1083
LguI	GCTCTTC	1 cut(s)	1098
LmnI	GCTCC	7 cut(s)	13, 25, 115, 702, 1747, 1884, 2150
LweI	GCATC	7 cut(s)	173, 797, 1420, 1442, 1900, 1951, 2578
MaeI	CTAG	5 cut(s)	279, 620, 1197, 2372, 2411
MaeII	ACGT	4 cut(s)	616, 1303, 1408, 1567
MboII	GAAGA	9 cut(s)	83, 408, 546, 956, 1085, 1519, 2306, 2363, 2527
MflI	RGATCY	8 cut(s)	79, 92, 836, 912, 1507, 1846, 2517, 2771
MhlI	GDGCHC	4 cut(s)	1093, 1501, 2067, 2494
MlsI	TGGCCA	1 cut(s)	602
MluNI	TGGCCA	1 cut(s)	602
MlyI	GAGTC	5 cut(s)	147, 395, 678, 1610, 1640
MmeI	TCCRAC	1 cut(s)	903
Mox20I	TGGCCA	1 cut(s)	602
Mph1103I	ATGCAT	1 cut(s)	1435
MroXI	GAANNNNTTC	1 cut(s)	2440
MscI	TGGCCA	1 cut(s)	602
MseI	TTAA	5 cut(s)	257, 1235, 1767, 1992, 2640
MslI	CAYNNNNRTG	2 cut(s)	1896, 2781
Msp20I	TGGCCA	1 cut(s)	602
MspA1I	CMGCKG	2 cut(s)	572, 2575
MspCI	CTTAAG	1 cut(s)	2639
MspI	CCGG	3 cut(s)	173, 1650, 1882
MspR9I	CCNGG	6 cut(s)	59, 90, 537, 1316, 1883, 1979
Mva1269I	GAATGC	1 cut(s)	2620
MvaI	CCWGG	5 cut(s)	59, 90, 537, 1316, 1979
MwoI	GCNNNNNNNGC	8 cut(s)	28, 246, 722, 731, 973, 1782, 2159, 2691
NciI	CCSGG	1 cut(s)	1883
NlaIII	CATG	7 cut(s)	405, 774, 823, 1385, 1798, 2463, 2549
NlaIV	GGNNCC	5 cut(s)	87, 111, 698, 1152, 1478
NmeAIII	GCCGAG	2 cut(s)	965, 2513
NmuCI	GTSAC	7 cut(s)	127, 155, 487, 1778, 1797, 2015, 2285
NsiI	ATGCAT	1 cut(s)	1435
NspI	RCATGY	1 cut(s)	774
NspV	TTCGAA	1 cut(s)	652
PaeR7I	CTCGAG	2 cut(s)	104, 2072
PagI	TCATGA	1 cut(s)	819
PceI	AGGCCT	1 cut(s)	1750
PciI	ACATGT	1 cut(s)	770
PciSI	GCTCTTC	1 cut(s)	1098
PctI	GAATGC	1 cut(s)	2620
PdmI	GAANNNNTTC	1 cut(s)	2440
PfeI	GAWTC	8 cut(s)	1052, 1184, 1268, 1518, 1721, 2124, 2193, 2240
PflFI	GACNNNGTC	1 cut(s)	929
PflMI	CCANNNNNTGG	1 cut(s)	348
PfoI	TCCNGGA	2 cut(s)	57, 1881
PleI	GAGTC	5 cut(s)	146, 394, 677, 1609, 1639
PpsI	GAGTC	5 cut(s)	146, 394, 677, 1609, 1639
Ppu21I	YACGTR	1 cut(s)	1568
PpuMI	RGGWCCY	1 cut(s)	311
PscI	ACATGT	1 cut(s)	770
PsiI	TTATAA	1 cut(s)	578
Psp124BI	GAGCTC	1 cut(s)	1093
Psp5II	RGGWCCY	1 cut(s)	311
Psp6I	CCWGG	5 cut(s)	57, 88, 535, 1314, 1977
PspEI	GGTNACC	1 cut(s)	487
PspFI	CCCAGC	1 cut(s)	1997
PspGI	CCWGG	5 cut(s)	57, 88, 535, 1314, 1977
PspN4I	GGNNCC	5 cut(s)	87, 111, 698, 1152, 1478
PspPI	GGNCC	6 cut(s)	55, 85, 170, 205, 311, 925
PspPPI	RGGWCCY	1 cut(s)	311
PspXI	VCTCGAGB	1 cut(s)	104
PstI	CTGCAG	4 cut(s)	54, 886, 1515, 1787
PstNI	CAGNNNCTG	2 cut(s)	542, 572
PsuI	RGATCY	8 cut(s)	79, 92, 836, 912, 1507, 1846, 2517, 2771
PsyI	GACNNNGTC	1 cut(s)	929
PvuII	CAGCTG	1 cut(s)	572
RsaI	GTAC	3 cut(s)	1152, 2267, 2730
RsaNI	GTAC	3 cut(s)	1151, 2266, 2729
RseI	CAYNNNNRTG	2 cut(s)	1896, 2781
SacI	GAGCTC	1 cut(s)	1093
SalI	GTCGAC	1 cut(s)	1284
SapI	GCTCTTC	1 cut(s)	1098
SaqAI	TTAA	5 cut(s)	257, 1235, 1767, 1992, 2640
Sau96I	GGNCC	6 cut(s)	55, 85, 170, 205, 311, 925
ScaI	AGTACT	1 cut(s)	2730
SchI	GAGTC	5 cut(s)	147, 395, 678, 1610, 1640
ScrFI	CCNGG	6 cut(s)	59, 90, 537, 1316, 1883, 1979
SduI	GDGCHC	4 cut(s)	1093, 1501, 2067, 2494
SfaNI	GCATC	7 cut(s)	173, 797, 1420, 1442, 1900, 1951, 2578
SfcI	CTRYAG	4 cut(s)	50, 882, 1511, 1783
Sfr274I	CTCGAG	2 cut(s)	104, 2072
SfuI	TTCGAA	1 cut(s)	652
SinI	GGWCC	6 cut(s)	55, 85, 170, 205, 311, 925
SlaI	CTCGAG	2 cut(s)	104, 2072
SmiMI	CAYNNNNRTG	2 cut(s)	1896, 2781
SmlI	CTYRAG	5 cut(s)	104, 856, 2060, 2072, 2639
SmoI	CTYRAG	5 cut(s)	104, 856, 2060, 2072, 2639
SnaBI	TACGTA	1 cut(s)	1568
SpeI	ACTAGT	1 cut(s)	2410
SseBI	AGGCCT	1 cut(s)	1750
SsiI	CCGC	3 cut(s)	250, 2575, 2618
SspI	AATATT	1 cut(s)	1582
SspMI	CTAG	5 cut(s)	279, 620, 1197, 2372, 2411
SstI	GAGCTC	1 cut(s)	1093
StuI	AGGCCT	1 cut(s)	1750
StyD4I	CCNGG	6 cut(s)	57, 88, 535, 1314, 1881, 1977
StyI	CCWWGG	3 cut(s)	1190, 1745, 1902
TaaI	ACNGT	7 cut(s)	127, 493, 609, 1938, 2235, 2291, 2728
TaiI	ACGT	4 cut(s)	619, 1306, 1411, 1570
TaqI	TCGA	8 cut(s)	105, 593, 652, 1285, 1908, 2073, 2122, 2743
TatI	WGTACW	2 cut(s)	2265, 2728
TauI	GCSGC	1 cut(s)	252
TfiI	GAWTC	8 cut(s)	1052, 1184, 1268, 1518, 1721, 2124, 2193, 2240
Tru1I	TTAA	5 cut(s)	257, 1235, 1767, 1992, 2640
Tru9I	TTAA	5 cut(s)	257, 1235, 1767, 1992, 2640
TscAI	CASTG	5 cut(s)	223, 436, 1943, 2184, 2296
TseFI	GTSAC	7 cut(s)	127, 155, 487, 1778, 1797, 2015, 2285
Tsp45I	GTSAC	7 cut(s)	127, 155, 487, 1778, 1797, 2015, 2285
TspGWI	ACGGA	6 cut(s)	197, 849, 1404, 1469, 1830, 1844
TspRI	CASTG	5 cut(s)	223, 436, 1943, 2184, 2296
Tth111I	GACNNNGTC	1 cut(s)	929
Van91I	CCANNNNNTGG	1 cut(s)	348
Vha464I	CTTAAG	1 cut(s)	2639
VpaK11BI	GGWCC	6 cut(s)	55, 85, 170, 205, 311, 925
XagI	CCTNNNNNAGG	1 cut(s)	592
XapI	RAATTY	8 cut(s)	496, 629, 1320, 1558, 2079, 2440, 2464, 2527
XceI	RCATGY	1 cut(s)	774
XhoI	CTCGAG	2 cut(s)	104, 2072
XmiI	GTMKAC	2 cut(s)	1285, 2172
XmnI	GAANNNNTTC	1 cut(s)	2440
XspI	CTAG	5 cut(s)	279, 620, 1197, 2372, 2411
ZraI	GACGTC	1 cut(s)	1409
ZrmI	AGTACT	1 cut(s)	2730
Zsp2I	ATGCAT	1 cut(s)	1435

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.