Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000006

Orthogroup ID

OG0003250

Physical Location & Seq

Genomic Coordinates Reverse (-)

61221651 .. 61224982

Gene Structure 3332 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug06G0495000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2466 bp

ATGATTAAGAATAATCCTACCGACCCTGTTTTTCTGCTCTTTTTCCTGTTCTTCAGGATTAATTTCTTGGCCTTGGCACAAAACACGACTATTCCAGTAAATGTGGGAGTGATTCTTGACTTGGATACCAGGTCTGGGAAATTGGGGCTGAGCTGCATCAACATGGCCCTCTCCGACTTCTATGCCTCTCATGTTTACTACCATACTAGGCTGGTCCTACACAATAGGGACTCTGTGGAAGATGTTGTTGTTGCAGCTGGTGCAGCTCTGGACTTGATAAACAATGTTAAAGTGCAAGCCATCATAGGGCCACAATCATCAACGCAAGCAAATTTCGTAACTGAACTTGGAGATAAAGCTCAGGTGCCCATAATCTCATTTTCAGCAACAAGCCCTTCCCTTGGGAATTCATACTTTATCCGAATTGCACAAAATGACACATCTCAAGTGAAAGTGATAAGCGCCATCATCCAAGCCTTTGGTTGGAGTGAAGCAGTGCCCATCAGCGTTGATGATGAGTTTGGAAAGGGAGTAATACCTTATCTGACTACTGCTTTGCAGGCTGTCGGTGCTCGCATCCCCTACTGGGTTGTCATTCCTTCAATGGCAACTGATAAACAAATAGATGCAGAGCTTGACGAGTTGATGAAAATGCCAACCAGGGTCTTCATAGTGCACATGTTGCCTTCTCTTGGCTCTCGACTTTTTTCTAGGGCAAATGCAATGGGAATGATGGAAAAAGGCTTTGCTTGGATAGTGACAAATGACATGACTAGCTTCTTTAGTTCCTCAAATTATTCTGTCATAGATAACATGCAAGGGGTGTTGGGTTTAAAGACTTACGTTCCAAATACGGAGCAGCTTGAAAATTTTAGAGGTAGATGGCAAAGGAAATTCCAACAAGACAATCCAACTCTCCTCAATGTTAAATTGGATGTTTTCGGAGTGTGGGCTTATGATGCGGCTTGGGCACTAGCCACGGCAGTTGAGAAGGTACAGAGTACAAACTTCTCTTCCCGCAAGATGAACATTTCTGGTAGTTCCACTGCAAGATTGGAGATCTCTCAGAGTGGTCCTGAACTTGTCCGAGAACTATCGGCTACGAGATTTACTGGACTTTCAGGAGATTTCAGTCTTATTAATGGACAACTACAATCATCAACTTTTCAGGTAGTTAATGTGAACGATAATGGGGAAAGAGGGGTTGGATATTGGACACCCCAGAACGGACTTGTAAGAAACTTAAACTCAAAAAACACAAGCAGCACTAACACAAGTAATGCCACTGGATACTGCATTGACGTCTTTAAAGCTGTAATAGAATCATTACCATACCCTGTCCCTTACGAGTTTTATCCCTTTGAAAACTCTCACCATCAGAGTGCTGGTACTTATAATGATTTGCTTGATCAAGTGTTTCTTGGGAACTATGATGCAGCAGTTGGTGATATTACCATTAGAGCAAATAGATCCTTGTATGTCGACTTCACATTGCCATACACAGAATCTGGGATATCAATGATCGTGCCTATCAAGGATAAAAGTGATGCTAAAAGCACATGGGTGTTTTTGAAGCCTTTGACTTGGGACCTTTGGGTAGCAATCGGTTGTTTTTTCATATTCATTGGTTTTGTAGTCTGGGTTCTTGAACACAGGATAAATAAAGACTTCCGAGGGCCTCCACTTCATCAAATTGGCACAAGCTTTTGGTTCTCATTCTCAACCATGGTTTTTGCACAAAGGGAGCGAGTACTGACCAACTTGGCTAGGTTTGTTGTGATAGTATGGTGCCTTGTTGTTCTCATACTAACTCAAAGTTATACCGCGAGTTTTAGTTCAATTTTAACAGTTCAACAGCTTCAACCAGTCATTACTGATGTAAACCAGCTTTTGAAAAACGGGAACAGTGTTGGCTGCTATTACACTTTTTATATTTATGATATTTTGAAAGAAATAGGATTTCAAGAGGCAAACCTTCGGGACTACGGGTCTCCAGAACAATTGGATGAAATTTTTCAAAATGGGAATGGAAAACATGGTATTTCGGCTGCTTTTGGTGAAACCCCGTACCTGAAACTTTTTCTTGCAACTTATTGTTCAAAATATGCCATAGTTGAGCCAACATTTAAATCTGATGGTTTTGCCTTTGTCTTCCCAAAAGGCTCGATTCTCACGCGTGATGTTTCAAGTGCAATCACTAGGTTAAAGGAGGAAGGTCTGATGGAGAAAATCGAGAACAAGTGGTTCAAGAAACAAGCAACTTGTTCAGAGTCCAGCACCACAAGTTCTTCCAACAGTCTTACTCTTAGCCTTGATAGCTTTTGGGGCCTCTTCATTGTTGCTGGGGTTGCTTCATCATTAGCTCTCCTCATATGTGCAGCCATGTTCTTGATGGGCCAGTCCCTGGGCCAGTCCCTCCTTAATTGGAGGAGAGAAAGAAAGAAAGCAGGAGACAAAGAAAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000226 GO:0001101 GO:0003674 GO:0003824 GO:0004721 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007010 GO:0007017 GO:0008138 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009987 GO:0010033 GO:0010119 GO:0010468 GO:0016043 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019222 GO:0019538 GO:0030865 GO:0031122 GO:0033993 GO:0036211 GO:0042221 GO:0042578 GO:0043170 GO:0043412 GO:0043622 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0060255 GO:0065007 GO:0071704 GO:0071840 GO:0097305 GO:0097435 GO:0140096 GO:1901564 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Act-Frag_cataly DSPc

Protein Analysis

821

Amino Acids

91.4

Weight (kDa)

6.21

Isoelectric Point (pI)

30.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peripla_BP_6	PF13458	32 - 398	1.2e-18	Periplasmic binding protein
ANF_receptor	PF01094	52 - 396	2.2e-76	Receptor family ligand binding region
Lig_chan-Glu_bd	PF10613	418 - 510	1.5e-11	Ligated ion channel L-glutamate- and glycine-binding site
SBP_bac_3	PF00497	424 - 750	4.5e-12	Bacterial extracellular solute-binding proteins, family 3
Lig_chan	PF00060	528 - 784	2.2e-38	Ligand-gated ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003250)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G23720 AT5G23720 AT5G23720
fragaria_vesca	FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220
malus_domestica	MD06G1132400.v1.1 MD14G1148800.v1.1
prunus_persica	Prupe.5G143400_v2.0.a1
pyrus_communis	pycom06g12400 pycom14g12360
rosa_chinensis	RchiOBHm_Chr4g0416471 RchiOBHm_Chr7g0188941 RchiOBHm_Chr7g0194331
rosa_laevigata	RLG00000004049 RLG00000004648
rosa_multiflora	Rmu_co8071116.1_g000001 Rmu_sc0007831.1_g000001 Rmu_sc0032883.1_g000001 Rmu_ssc0000113.1_g000006
rosa_roxburghii	Rroxscaffold_3G00265920
rosa_rugosa	Rorug06G0494800 Rorug06G0494900 Rorug06G0495000
rosa_samantha	Rh7AG099700 Rh7BG101800 Rh7CG103100 Rh7CG146600 Rh7DG101600 Rh7DG144200
rosa_wichuraiana	Rw7G008590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1395
AatII	GACGTC	1 cut(s)	1305
AccB1I	GGYRCC	2 cut(s)	364, 1788
AccB7I	CCANNNNNTGG	1 cut(s)	2404
AccI	GTMKAC	1 cut(s)	1482
AccII	CGCG	2 cut(s)	1826, 2176
AciI	CCGC	3 cut(s)	962, 1018, 1824
AclWI	GGATC	1 cut(s)	1464
AcsI	RAATTY	5 cut(s)	331, 406, 868, 893, 2010
AcuI	CTGAAG	1 cut(s)	37
AcyI	GRCGYC	1 cut(s)	1302
AfaI	GTAC	5 cut(s)	996, 1003, 1390, 1752, 2069
AfiI	CCNNNNNNNGG	8 cut(s)	135, 306, 401, 483, 586, 692, 1226, 2404
AflIII	ACRYGT	2 cut(s)	678, 2174
AhdI	GACNNNNNGTC	1 cut(s)	1987
AjnI	CCWGG	3 cut(s)	128, 659, 2403
AjuI	GAANNNNNNNTTGG	2 cut(s)	1188, 1220
AleI	CACNNNNGTG	1 cut(s)	1562
Alw21I	GWGCWC	2 cut(s)	574, 678
Alw26I	GTCTC	2 cut(s)	1995, 2445
Alw44I	GTGCAC	1 cut(s)	674
AlwI	GGATC	1 cut(s)	1464
AlwNI	CAGNNNCTG	2 cut(s)	1508, 2404
AoxI	GGCC	7 cut(s)	69, 165, 308, 1676, 2326, 2395, 2407
ApaLI	GTGCAC	1 cut(s)	674
ApeKI	GCWGC	9 cut(s)	153, 254, 263, 859, 1263, 1436, 1914, 2048, 2378
ApoI	RAATTY	5 cut(s)	331, 406, 868, 893, 2010
ArsI	GACNNNNNNTTYG	2 cut(s)	538, 570
AseI	ATTAAT	2 cut(s)	60, 1140
Asp700I	GAANNNNTTC	1 cut(s)	2013
AspLEI	GCGC	1 cut(s)	464
AspS9I	GGNCC	9 cut(s)	166, 214, 308, 1073, 1588, 1676, 2326, 2395, 2407
AsuHPI	GGTGA	3 cut(s)	1364, 1457, 2069
AvaII	GGWCC	3 cut(s)	214, 1073, 1588
BaeGI	GKGCMC	4 cut(s)	369, 501, 678, 973
BanI	GGYRCC	2 cut(s)	364, 1788
BarI	GAAGNNNNNNTAC	2 cut(s)	1469, 1501
BbsI	GAAGAC	2 cut(s)	658, 2143
Bbv12I	GWGCWC	2 cut(s)	574, 678
BbvI	GCAGC	9 cut(s)	140, 266, 275, 871, 1275, 1448, 1901, 2035, 2390
BccI	CCATC	9 cut(s)	308, 473, 509, 727, 876, 1383, 2129, 2215, 2386
BceAI	ACGGC	1 cut(s)	996
BcgI	CGANNNNNNTGC	2 cut(s)	164, 198
BciT130I	CCWGG	3 cut(s)	130, 661, 2405
BciVI	GTATCC	2 cut(s)	118, 1283
BclI	TGATCA	1 cut(s)	1408
BcoDI	GTCTC	2 cut(s)	1995, 2445
BfaI	CTAG	6 cut(s)	207, 711, 774, 974, 1767, 2199
BfoI	RGCGCY	1 cut(s)	465
BfuI	GTATCC	2 cut(s)	118, 1283
BglII	AGATCT	1 cut(s)	1059
BlpI	GCTNAGC	1 cut(s)	149
BmcAI	AGTACT	1 cut(s)	1752
Bme1390I	CCNGG	3 cut(s)	130, 661, 2405
Bme18I	GGWCC	3 cut(s)	214, 1073, 1588
BmeRI	GACNNNNNGTC	1 cut(s)	1987
BmgT120I	GGNCC	9 cut(s)	166, 214, 308, 1073, 1588, 1676, 2326, 2395, 2407
BmiI	GGNNCC	4 cut(s)	366, 1589, 1790, 2327
BmrFI	CCNGG	3 cut(s)	130, 661, 2405
BmrI	ACTGGG	1 cut(s)	595
BmsI	GCATC	6 cut(s)	165, 585, 616, 949, 1423, 1537
BmuI	ACTGGG	1 cut(s)	595
BpiI	GAAGAC	2 cut(s)	658, 2143
BpmI	CTGGAG	1 cut(s)	1977
Bpu10I	CCTNAGC	1 cut(s)	360
Bpu1102I	GCTNAGC	1 cut(s)	149
BpuEI	CTTGAG	1 cut(s)	429
BsaHI	GRCGYC	1 cut(s)	1302
BsaI	GGTCTC	1 cut(s)	1995
BsaJI	CCNNGG	8 cut(s)	72, 400, 660, 978, 1672, 1725, 2403, 2404
Bsc4I	CCNNNNNNNGG	8 cut(s)	135, 306, 401, 483, 586, 692, 1226, 2404
Bse1I	ACTGG	7 cut(s)	95, 590, 1117, 1291, 1865, 2398, 2410
Bse3DI	GCAATG	2 cut(s)	729, 1490
BseBI	CCWGG	3 cut(s)	130, 661, 2405
BseDI	CCNNGG	8 cut(s)	72, 400, 660, 978, 1672, 1725, 2403, 2404
BseGI	GGATG	4 cut(s)	468, 576, 940, 2011
BseLI	CCNNNNNNNGG	8 cut(s)	135, 306, 401, 483, 586, 692, 1226, 2404
BseMI	GCAATG	2 cut(s)	729, 1490
BseMII	CTCAG	3 cut(s)	140, 374, 1079
BseNI	ACTGG	7 cut(s)	95, 590, 1117, 1291, 1865, 2398, 2410
BseRI	GAGGAG	3 cut(s)	908, 2357, 2443
BseSI	GKGCMC	4 cut(s)	369, 501, 678, 973
BseXI	GCAGC	9 cut(s)	140, 266, 275, 871, 1275, 1448, 1901, 2035, 2390
BseYI	CCCAGC	1 cut(s)	2342
BsgI	GTGCAG	2 cut(s)	282, 2397
Bsh1236I	CGCG	2 cut(s)	1826, 2176
BshFI	GGCC	7 cut(s)	71, 167, 310, 1678, 2328, 2397, 2409
BshNI	GGYRCC	2 cut(s)	364, 1788
BsiHKAI	GWGCWC	2 cut(s)	574, 678
BslFI	GGGAC	6 cut(s)	242, 1325, 1601, 1994, 2386, 2398
BslI	CCNNNNNNNGG	8 cut(s)	135, 306, 401, 483, 586, 692, 1226, 2404
BsmAI	GTCTC	2 cut(s)	1995, 2445
BsmFI	GGGAC	6 cut(s)	242, 1325, 1601, 1994, 2386, 2398
BsnI	GGCC	7 cut(s)	71, 167, 310, 1678, 2328, 2397, 2409
Bso31I	GGTCTC	1 cut(s)	1995
Bsp1286I	GDGCHC	5 cut(s)	369, 501, 574, 678, 973
Bsp143I	GATC	4 cut(s)	1059, 1408, 1469, 1521
Bsp1720I	GCTNAGC	1 cut(s)	149
Bsp19I	CCATGG	1 cut(s)	1725
BspACI	CCGC	3 cut(s)	962, 1018, 1824
BspANI	GGCC	7 cut(s)	71, 167, 310, 1678, 2328, 2397, 2409
BspCNI	CTCAG	3 cut(s)	141, 373, 1078
BspFNI	CGCG	2 cut(s)	1826, 2176
BspLI	GGNNCC	4 cut(s)	366, 1589, 1790, 2327
BspPI	GGATC	1 cut(s)	1464
BspT107I	GGYRCC	2 cut(s)	364, 1788
BspTNI	GGTCTC	1 cut(s)	1995
BsrDI	GCAATG	2 cut(s)	729, 1490
BsrI	ACTGG	7 cut(s)	95, 590, 1117, 1291, 1865, 2398, 2410
BssECI	CCNNGG	8 cut(s)	72, 400, 660, 978, 1672, 1725, 2403, 2404
BssMI	GATC	4 cut(s)	1059, 1408, 1469, 1521
BssNI	GRCGYC	1 cut(s)	1302
BssT1I	CCWWGG	3 cut(s)	72, 400, 1725
Bst2UI	CCWGG	3 cut(s)	130, 661, 2405
Bst4CI	ACNGT	3 cut(s)	1849, 1907, 2297
Bst6I	CTCTTC	2 cut(s)	1018, 2336
BstACI	GRCGYC	1 cut(s)	1302
BstAPI	GCANNNNNTGC	2 cut(s)	260, 682
BstC8I	GCNNGC	4 cut(s)	297, 327, 561, 574
BstDEI	CTNAG	4 cut(s)	149, 360, 1065, 2306
BstDSI	CCRYGG	2 cut(s)	978, 1725
BstF5I	GGATG	4 cut(s)	468, 576, 940, 2011
BstFNI	CGCG	2 cut(s)	1826, 2176
BstH2I	RGCGCY	1 cut(s)	465
BstHHI	GCGC	1 cut(s)	464
BstKTI	GATC	4 cut(s)	1062, 1411, 1472, 1524
BstMAI	GTCTC	2 cut(s)	1995, 2445
BstMBI	GATC	4 cut(s)	1059, 1408, 1469, 1521
BstNI	CCWGG	3 cut(s)	130, 661, 2405
BstNSI	RCATGY	2 cut(s)	682, 817
BstSCI	CCNGG	3 cut(s)	128, 659, 2403
BstSLI	GKGCMC	4 cut(s)	369, 501, 678, 973
BstUI	CGCG	2 cut(s)	1826, 2176
BstV1I	GCAGC	9 cut(s)	140, 266, 275, 871, 1275, 1448, 1901, 2035, 2390
BstV2I	GAAGAC	2 cut(s)	658, 2143
BstX2I	RGATCY	2 cut(s)	1059, 1469
BstXI	CCANNNNNNTGG	1 cut(s)	479
BstYI	RGATCY	2 cut(s)	1059, 1469
BsuI	GTATCC	2 cut(s)	118, 1283
BsuRI	GGCC	7 cut(s)	71, 167, 310, 1678, 2328, 2397, 2409
BtgI	CCRYGG	2 cut(s)	978, 1725
BtsCI	GGATG	4 cut(s)	468, 576, 940, 2011
BtsI	GCAGTG	2 cut(s)	501, 1044
BtsIMutI	CAGTG	4 cut(s)	501, 1044, 1284, 1912
Cac8I	GCNNGC	4 cut(s)	297, 327, 561, 574
CaiI	CAGNNNCTG	2 cut(s)	1508, 2404
CfoI	GCGC	1 cut(s)	464
Cfr13I	GGNCC	9 cut(s)	166, 214, 308, 1073, 1588, 1676, 2326, 2395, 2407
CsiI	ACCWGGT	1 cut(s)	128
Csp6I	GTAC	5 cut(s)	995, 1002, 1389, 1751, 2068
CspCI	CAANNNNNGTGG	2 cut(s)	967, 1002
CviAII	CATG	9 cut(s)	163, 191, 679, 769, 814, 1560, 1726, 2036, 2383
CviQI	GTAC	5 cut(s)	995, 1002, 1389, 1751, 2068
DdeI	CTNAG	4 cut(s)	149, 360, 1065, 2306
DpnI	GATC	4 cut(s)	1061, 1410, 1471, 1523
DpnII	GATC	4 cut(s)	1059, 1408, 1469, 1521
DraI	TTTAAA	3 cut(s)	834, 1309, 2128
DriI	GACNNNNNGTC	1 cut(s)	1987
Eam1104I	CTCTTC	2 cut(s)	1018, 2336
Eam1105I	GACNNNNNGTC	1 cut(s)	1987
EarI	CTCTTC	2 cut(s)	1018, 2336
Eco130I	CCWWGG	3 cut(s)	72, 400, 1725
Eco31I	GGTCTC	1 cut(s)	1995
Eco32I	GATATC	1 cut(s)	1515
Eco47I	GGWCC	3 cut(s)	214, 1073, 1588
Eco57I	CTGAAG	1 cut(s)	37
EcoO109I	RGGNCCY	3 cut(s)	1588, 1676, 2326
EcoRI	GAATTC	1 cut(s)	406
EcoRII	CCWGG	3 cut(s)	128, 659, 2403
EcoRV	GATATC	1 cut(s)	1515
EcoT14I	CCWWGG	3 cut(s)	72, 400, 1725
ErhI	CCWWGG	3 cut(s)	72, 400, 1725
FaeI	CATG	9 cut(s)	166, 194, 682, 772, 817, 1563, 1729, 2039, 2386
FalI	AAGNNNNNCTT	2 cut(s)	1404, 1436
FaqI	GGGAC	6 cut(s)	242, 1325, 1601, 1994, 2386, 2398
FatI	CATG	9 cut(s)	162, 190, 678, 768, 813, 1559, 1725, 2035, 2382
FauI	CCCGC	1 cut(s)	1025
FauNDI	CATATG	1 cut(s)	2372
FbaI	TGATCA	1 cut(s)	1408
FblI	GTMKAC	1 cut(s)	1482
FokI	GGATG	4 cut(s)	455, 563, 947, 2018
FspBI	CTAG	6 cut(s)	207, 711, 774, 974, 1767, 2199
GlaI	GCGC	1 cut(s)	463
GsaI	CCCAGC	1 cut(s)	2346
GsuI	CTGGAG	1 cut(s)	1977
HaeII	RGCGCY	1 cut(s)	465
HaeIII	GGCC	7 cut(s)	71, 167, 310, 1678, 2328, 2397, 2409
HhaI	GCGC	1 cut(s)	464
Hin1I	GRCGYC	1 cut(s)	1302
Hin1II	CATG	9 cut(s)	166, 194, 682, 772, 817, 1563, 1729, 2039, 2386
Hin6I	GCGC	1 cut(s)	462
HinP1I	GCGC	1 cut(s)	462
HincII	GTYRAC	1 cut(s)	1483
HindII	GTYRAC	1 cut(s)	1483
HindIII	AAGCTT	1 cut(s)	1702
HinfI	GANTC	6 cut(s)	112, 230, 1322, 1505, 2167, 2270
HphI	GGTGA	3 cut(s)	1364, 1457, 2069
Hpy166II	GTNNAC	5 cut(s)	196, 676, 1183, 1483, 1882
Hpy8I	GTNNAC	5 cut(s)	196, 676, 1183, 1483, 1882
HpyAV	CCTTC	6 cut(s)	405, 609, 696, 985, 1985, 2207
HpyCH4III	ACNGT	3 cut(s)	1849, 1907, 2297
HpyCH4IV	ACGT	2 cut(s)	843, 1302
HpyF3I	CTNAG	4 cut(s)	149, 360, 1065, 2306
HpySE526I	ACGT	2 cut(s)	843, 1302
Hsp92I	GRCGYC	1 cut(s)	1302
Hsp92II	CATG	9 cut(s)	166, 194, 682, 772, 817, 1563, 1729, 2039, 2386
HspAI	GCGC	1 cut(s)	462
Ksp22I	TGATCA	1 cut(s)	1408
Kzo9I	GATC	4 cut(s)	1059, 1408, 1469, 1521
LmnI	GCTCC	2 cut(s)	856, 1744
Lsp1109I	GCAGC	9 cut(s)	140, 266, 275, 871, 1275, 1448, 1901, 2035, 2390
LweI	GCATC	6 cut(s)	165, 585, 616, 949, 1423, 1537
MabI	ACCWGGT	1 cut(s)	128
MaeI	CTAG	6 cut(s)	207, 711, 774, 974, 1767, 2199
MaeII	ACGT	2 cut(s)	843, 1302
MaeIII	GTNAC	2 cut(s)	337, 757
MalI	GATC	4 cut(s)	1061, 1410, 1471, 1523
MboI	GATC	4 cut(s)	1059, 1408, 1469, 1521
MboII	GAAGA	7 cut(s)	43, 251, 658, 1005, 2143, 2280, 2323
MfeI	CAATTG	1 cut(s)	2000
MflI	RGATCY	2 cut(s)	1059, 1469
MhlI	GDGCHC	5 cut(s)	369, 501, 574, 678, 973
MluI	ACGCGT	1 cut(s)	2174
MlyI	GAGTC	2 cut(s)	224, 2279
MmeI	TCCRAC	6 cut(s)	198, 464, 922, 935, 1186, 2316
MroXI	GAANNNNTTC	1 cut(s)	2013
MslI	CAYNNNNRTG	3 cut(s)	161, 1380, 1562
MspA1I	CMGCKG	1 cut(s)	257
MspR9I	CCNGG	3 cut(s)	130, 661, 2405
MunI	CAATTG	1 cut(s)	2000
MvaI	CCWGG	3 cut(s)	130, 661, 2405
MvnI	CGCG	2 cut(s)	1826, 2176
NcoI	CCATGG	1 cut(s)	1725
NdeI	CATATG	1 cut(s)	2372
NdeII	GATC	4 cut(s)	1059, 1408, 1469, 1521
NlaIII	CATG	9 cut(s)	166, 194, 682, 772, 817, 1563, 1729, 2039, 2386
NlaIV	GGNNCC	4 cut(s)	366, 1589, 1790, 2327
NmuCI	GTSAC	1 cut(s)	757
NspI	RCATGY	2 cut(s)	682, 817
OliI	CACNNNNGTG	1 cut(s)	1562
PasI	CCCWGGG	1 cut(s)	2404
PciI	ACATGT	1 cut(s)	678
PdmI	GAANNNNTTC	1 cut(s)	2013
PfeI	GAWTC	4 cut(s)	112, 1322, 1505, 2167
PflMI	CCANNNNNTGG	1 cut(s)	2404
PleI	GAGTC	2 cut(s)	224, 2278
PpsI	GAGTC	2 cut(s)	224, 2278
PpuMI	RGGWCCY	1 cut(s)	1588
PscI	ACATGT	1 cut(s)	678
PshBI	ATTAAT	2 cut(s)	60, 1140
PsiI	TTATAA	1 cut(s)	1395
Psp5II	RGGWCCY	1 cut(s)	1588
Psp6I	CCWGG	3 cut(s)	128, 659, 2403
PspFI	CCCAGC	1 cut(s)	2342
PspGI	CCWGG	3 cut(s)	128, 659, 2403
PspN4I	GGNNCC	4 cut(s)	366, 1589, 1790, 2327
PspPI	GGNCC	9 cut(s)	166, 214, 308, 1073, 1588, 1676, 2326, 2395, 2407
PspPPI	RGGWCCY	1 cut(s)	1588
PsrI	GAACNNNNNNTAC	2 cut(s)	1626, 1658
PstNI	CAGNNNCTG	2 cut(s)	1508, 2404
PsuI	RGATCY	2 cut(s)	1059, 1469
PvuII	CAGCTG	1 cut(s)	257
RsaI	GTAC	5 cut(s)	996, 1003, 1390, 1752, 2069
RsaNI	GTAC	5 cut(s)	995, 1002, 1389, 1751, 2068
RseI	CAYNNNNRTG	3 cut(s)	161, 1380, 1562
SalI	GTCGAC	1 cut(s)	1481
Sau3AI	GATC	4 cut(s)	1059, 1408, 1469, 1521
Sau96I	GGNCC	9 cut(s)	166, 214, 308, 1073, 1588, 1676, 2326, 2395, 2407
ScaI	AGTACT	1 cut(s)	1752
SchI	GAGTC	2 cut(s)	224, 2279
ScrFI	CCNGG	3 cut(s)	130, 661, 2405
SduI	GDGCHC	5 cut(s)	369, 501, 574, 678, 973
SexAI	ACCWGGT	1 cut(s)	128
SfaNI	GCATC	6 cut(s)	165, 585, 616, 949, 1423, 1537
SinI	GGWCC	3 cut(s)	214, 1073, 1588
SmiI	ATTTAAAT	1 cut(s)	2128
SmiMI	CAYNNNNRTG	3 cut(s)	161, 1380, 1562
SmlI	CTYRAG	1 cut(s)	444
SmoI	CTYRAG	1 cut(s)	444
SsiI	CCGC	3 cut(s)	962, 1018, 1824
SspMI	CTAG	6 cut(s)	207, 711, 774, 974, 1767, 2199
StyD4I	CCNGG	3 cut(s)	128, 659, 2403
StyI	CCWWGG	3 cut(s)	72, 400, 1725
SwaI	ATTTAAAT	1 cut(s)	2128
TaaI	ACNGT	3 cut(s)	1849, 1907, 2297
TaiI	ACGT	2 cut(s)	846, 1305
TaqI	TCGA	4 cut(s)	700, 1482, 2165, 2232
TatI	WGTACW	2 cut(s)	1001, 1750
TauI	GCSGC	1 cut(s)	965
TfiI	GAWTC	4 cut(s)	112, 1322, 1505, 2167
TscAI	CASTG	4 cut(s)	501, 1051, 1291, 1912
TseFI	GTSAC	1 cut(s)	757
TseI	GCWGC	9 cut(s)	153, 254, 263, 859, 1263, 1436, 1914, 2048, 2378
Tsp45I	GTSAC	1 cut(s)	757
TspGWI	ACGGA	2 cut(s)	869, 1242
TspRI	CASTG	4 cut(s)	501, 1051, 1291, 1912
Van91I	CCANNNNNTGG	1 cut(s)	2404
VneI	GTGCAC	1 cut(s)	674
VpaK11BI	GGWCC	3 cut(s)	214, 1073, 1588
VspI	ATTAAT	2 cut(s)	60, 1140
XapI	RAATTY	5 cut(s)	331, 406, 868, 893, 2010
XceI	RCATGY	2 cut(s)	682, 817
XcmI	CCANNNNNNNNNTGG	3 cut(s)	1051, 1382, 2389
XmiI	GTMKAC	1 cut(s)	1482
XmnI	GAANNNNTTC	1 cut(s)	2013
XspI	CTAG	6 cut(s)	207, 711, 774, 974, 1767, 2199
ZraI	GACGTC	1 cut(s)	1303
ZrmI	AGTACT	1 cut(s)	1752

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.