Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7D

Orthogroup ID

OG0003250

Physical Location & Seq

Genomic Coordinates Reverse (-)

8482246 .. 8487345

Gene Structure 5100 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7DG101600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2787 bp

ATGGCAAAACAGGAGCAAAAGCAGGAGCAGCCTGCAATCATCAACCCTCTGCAGGTCCAGGAAGACGGGGATCTGGACCCTGGATCTGACGACCTCGAGGCTCCTCTGCCTCTCACCGTCACTTCCCGGGTCTTGTATATGTTGGGTGACATTGCGTCTGGACCGGCCTTCAAAATCAGTCAATGGCTGGAATTGGTCCGTAAACGCAGTGCCAAATACCATTCCTCTGGCTTCCCTCGCCGCCCATTAAGGCTTGACACAATGCCTGCTAGTGCAGGAGAGTGTAGTGAGGACTGGAAAGGTCCTGCGTCGCCTGAGCAAACCACAGAAGTCAATCTCTGGGAAAGACTTGGTAAAGCTGCTACATTGGACATTGAGTCGAGTTTTTTCTCATGGGATAGGCTTTCTTCACTTCACCACACTGAACATAGTAGTAGCAATGATCAGTCTGAGGATGAAATGAACAAAGCGCTGGAGGTCACTGTGAATTCGGGGGGAGTTGTTTTCTTTGCCCTTTTCAACCAGCCAGGGACTGATGATAACTCTTCTAAGGAAGCAGCGGCTGTTATAAAGATATCTTCATCGAGGATGGCCACACAGTCTGAACGTCTAGGTTACGAATTTGCAAAGTGGCTTGGAGTTCGAACTCCACAGGCCAGAGTCATACATAATTGTAGTATGGAGTGGCTCCAGATGAAGGAAGCAGCAGAAAAAGCAAGGGAAGCAGCAGTTTCAGAAGGAGATGAATGTGGGGAAATGACATGTTCGGAACTTTTGGAAGCTCTTGAACTTAGCCGATGCCTTTTGCTCATGAGCTATGTTCACGGATCTCCTTTACTTGAAAGCTCAAGTGTATTTGAGGTGAAGGAAACTGCAGAGAAAACAGCAGCTTCTCTTGGTAGGATCTTGATGTTGGACCTTGTCATCAGAAATGAAGATAGACTACCTTGCCGTCAGCTCAGATGGCGTGGAAACTCGGCAAATCTATTGTTTGCAGACAAAATGGATTTTGGAAATATGGACAGATTGGAGGAAGCCTTTGATTCTGCCATCAAGAGATACAAACCAAGAGTGATCAGAGCTCTTCAAAAGGATAGAAGGGCAACTTCAGTAGATATCAGATTGCATAATACAGCATTGGTACCACAGAAATCTGATCTTTCTGACATTATGGATTCGCCAAGGTCTAGTCAGATGAGCGTAAAGAGTCAACTCTCAGATGATTTAATGTTTTCCGATTTTCCAGTTGTGGCTATTGATTCTGGCGTTCCTCGTCGACCTCCTGCTGGAAAACGTGCAAATGACCAGGAAATTTATCCAAAGTTGGTTGAGTTACTACTGAACAATTCTGAATACTCCTCTAATCTGTTACATGACATAACATTAGGGAAATTAGGACGTCCTCCGTTACAAGATACTGATGCATCTGATACACAAGCAATGGAAATGACTTTGGTTGTTCAAGGGTTCCGTAGTGGGTTCAAACGTGCTCTCAGGGATCTGCAAGGATTCCATATCTTCTTACTGACACTTCATCAAAAACTGGAGAATCTGATACGTATATTTTTCAATATTATAGATAAAATAGGGGAGTCCGATAAGGAGGATTTGGTAGTTATTGAGTCTCCATCACAAGCTGCCGGAAGTGGTTATTTTCCTTCTACACCAAATAAGGAGAGGCTTGTCAATGAGAACCATTCAGATTTTAGTGATTCCGAATTGCAGAGAACTGCTCCAAGGCCTTCAACTTCAGGACCTAAAGAAAGCTGTGACTGCAGCTCTCCCATGTCACGGGAGAGTTGGCACGGAAAGTTTTGGAAAGGGAGTGCAAGTTCAGATCCCCTCCGTAGTCTGCGTTTGACAGCAAAGCTCCGGGACTTTCATAAAAATGCCAAGGTTGATGCTGAAACAAACAAAGAATTGGAACAGTGGAATGAAATGCTAAAGAGCGATGCAGTCAAACTTTGCCAGGAGAACAATTTTAATGCTGGGTTCTTTGAGGGTAGTGACAATAACGGCGTAGTTGACGCTTATGAATTGAAGGTCAGACTTGAGCACATTCTCGAGAGAATTTCATTGATATCTGAGGCTGCAAATACAGAGAGGCCATCTCGAATCACAAGTAGCCTGTTTATTGGTGGAGCGCTTGCTGCACGATCTGTATACACACTGCAACGCTTAGGAATCACTCATATATTATGTTTGTGTTCCAATGAAATTGGACAGTCAGATTCCCAGTTTCCTGATCTATTTGAGTACAAAAACTTCTCTATATGTGACAGTGAAGATTTCAACATCAGCAACATCTTTGATGAAGCTGTTGAGTTTATTGATCGTGTTGAAGAAACAGGAGGGAAGGTTCTAGTCCATTGCTTTGAGGGAAAAAGTAGAAGTGCTACACTAGTGCTTGCATACCTAATGCTCAGAAAGAATTCCACTCTATTAGAAGCATGGAATTCTCTAAAGCAGGTTCATCGCCGAGCACAACCCAATGATGGGTTTGCGAAGATCTTGTTGAATTTGGATAAGAAACTGCATGGTAAAGTTTCAATGGAGTGGCATCAGCGGAAGCCAATGATGAAAGTTTGCCCCATCTGTGGGGTGAATGCGGGTCTGAGTAGCAGCTCTCTTAAGCTTCATTTACAGAAATCACATAAGAAACTATCGTCTGGTAGCGTGGATAGCGCAATGACAATGGAAATACAAAAGGCTTTGACAGTACTAAAAATCAGTCGAGGCGGAAGTGTCAGCCCCACACAAAGATCTCATTCAGATGTTGAAGAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000226 GO:0001101 GO:0003674 GO:0003824 GO:0004721 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007010 GO:0007017 GO:0008138 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009987 GO:0010033 GO:0010119 GO:0010468 GO:0016043 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019222 GO:0019538 GO:0030865 GO:0031122 GO:0033993 GO:0036211 GO:0042221 GO:0042578 GO:0043170 GO:0043412 GO:0043622 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0060255 GO:0065007 GO:0071704 GO:0071840 GO:0097305 GO:0097435 GO:0140096 GO:1901564 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Act-Frag_cataly DSPc

Protein Analysis

928

Amino Acids

103.71

Weight (kDa)

5.82

Isoelectric Point (pI)

54.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Act-Frag_cataly	PF09192	110 - 423	1e-46	Actin-fragmin kinase, catalytic
DSPc	PF00782	712 - 842	1.3e-24	Dual specificity phosphatase, catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0003250)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G23720 AT5G23720 AT5G23720
fragaria_vesca	FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220
malus_domestica	MD06G1132400.v1.1 MD14G1148800.v1.1
prunus_persica	Prupe.5G143400_v2.0.a1
pyrus_communis	pycom06g12400 pycom14g12360
rosa_chinensis	RchiOBHm_Chr4g0416471 RchiOBHm_Chr7g0188941 RchiOBHm_Chr7g0194331
rosa_laevigata	RLG00000004049 RLG00000004648
rosa_multiflora	Rmu_co8071116.1_g000001 Rmu_sc0007831.1_g000001 Rmu_sc0032883.1_g000001 Rmu_ssc0000113.1_g000006
rosa_roxburghii	Rroxscaffold_3G00265920
rosa_rugosa	Rorug06G0494800 Rorug06G0494900 Rorug06G0495000
rosa_samantha	Rh7AG099700 Rh7BG101800 Rh7CG103100 Rh7CG146600 Rh7DG101600 Rh7DG144200
rosa_wichuraiana	Rw7G008590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	569
AatII	GACGTC	1 cut(s)	1402
AbsI	CCTCGAGG	1 cut(s)	95
Acc36I	ACCTGC	2 cut(s)	43, 2458
Acc65I	GGTACC	1 cut(s)	1141
AccB1I	GGYRCC	1 cut(s)	1141
AccI	GTMKAC	2 cut(s)	1276, 2163
AciI	CCGC	5 cut(s)	241, 560, 2566, 2609, 2739
AclWI	GGATC	6 cut(s)	78, 91, 835, 911, 1506, 1832
AcoI	YGGCCR	1 cut(s)	591
AcsI	RAATTY	7 cut(s)	487, 620, 1311, 2070, 2431, 2455, 2518
AcuI	CTGAAG	2 cut(s)	1092, 1734
AcyI	GRCGYC	1 cut(s)	1399
AfaI	GTAC	3 cut(s)	1143, 2258, 2721
AfeI	AGCGCT	2 cut(s)	471, 2145
AfiI	CCNNNNNNNGG	8 cut(s)	52, 697, 1286, 1791, 2495, 2496, 2597, 2598
AflII	CTTAAG	1 cut(s)	2630
AflIII	ACRYGT	1 cut(s)	761
AhdI	GACNNNNNGTC	2 cut(s)	154, 376
AhlI	ACTAGT	1 cut(s)	2401
AjnI	CCWGG	5 cut(s)	57, 79, 526, 1305, 1968
AloI	GAACNNNNNNTCC	4 cut(s)	1816, 1848, 2343, 2375
Alw21I	GWGCWC	4 cut(s)	1084, 1492, 2058, 2485
Alw26I	GTCTC	1 cut(s)	1629
AlwI	GGATC	6 cut(s)	78, 91, 835, 911, 1506, 1832
AlwNI	CAGNNNCTG	2 cut(s)	533, 563
Ama87I	CYCGRG	3 cut(s)	95, 126, 2063
Aor51HI	AGCGCT	2 cut(s)	471, 2145
AoxI	GGCC	5 cut(s)	165, 591, 654, 1739, 2105
ApoI	RAATTY	7 cut(s)	487, 620, 1311, 2070, 2431, 2455, 2518
Asp700I	GAANNNNTTC	1 cut(s)	2431
Asp718I	GGTACC	1 cut(s)	1141
AspLEI	GCGC	3 cut(s)	472, 2146, 2687
AspS9I	GGNCC	7 cut(s)	55, 76, 161, 196, 302, 916, 1754
AsuC2I	CCSGG	3 cut(s)	127, 128, 1874
AsuHPI	GGTGA	5 cut(s)	106, 158, 407, 874, 2614
AsuII	TTCGAA	1 cut(s)	643
AvaI	CYCGRG	3 cut(s)	95, 126, 2063
AvaII	GGWCC	7 cut(s)	55, 76, 161, 196, 302, 916, 1754
BalI	TGGCCA	1 cut(s)	593
BanI	GGYRCC	1 cut(s)	1141
BanII	GRGCYC	1 cut(s)	1084
BarI	GAAGNNNNNNTAC	4 cut(s)	927, 959, 2249, 2281
BbsI	GAAGAC	1 cut(s)	69
Bbv12I	GWGCWC	4 cut(s)	1084, 1492, 2058, 2485
BccI	CCATC	7 cut(s)	583, 957, 1058, 1636, 2116, 2489, 2600
BceAI	ACGGC	2 cut(s)	936, 2032
BcgI	CGANNNNNNTGC	2 cut(s)	2456, 2490
BciT130I	CCWGG	5 cut(s)	59, 81, 528, 1307, 1970
BclI	TGATCA	2 cut(s)	442, 1074
BcnI	CCSGG	3 cut(s)	127, 128, 1874
BcoDI	GTCTC	1 cut(s)	1629
BcuI	ACTAGT	1 cut(s)	2401
BfaI	CTAG	5 cut(s)	270, 611, 1188, 2363, 2402
BfmI	CTRYAG	3 cut(s)	50, 873, 1774
BfoI	RGCGCY	2 cut(s)	473, 2147
BfrI	CTTAAG	1 cut(s)	2630
BfuAI	ACCTGC	2 cut(s)	43, 2458
BglII	AGATCT	2 cut(s)	2508, 2762
BmcAI	AGTACT	1 cut(s)	2721
Bme1390I	CCNGG	8 cut(s)	59, 81, 127, 128, 528, 1307, 1874, 1970
Bme18I	GGWCC	7 cut(s)	55, 76, 161, 196, 302, 916, 1754
BmeRI	GACNNNNNGTC	2 cut(s)	154, 376
BmeT110I	CYCGRG	3 cut(s)	95, 126, 2063
BmgT120I	GGNCC	7 cut(s)	55, 76, 161, 196, 302, 916, 1754
BmiI	GGNNCC	5 cut(s)	78, 102, 689, 1143, 1469
BmrFI	CCNGG	8 cut(s)	59, 81, 127, 128, 528, 1307, 1874, 1970
BmrI	ACTGGG	1 cut(s)	2230
BmsI	GCATC	6 cut(s)	788, 1411, 1433, 1891, 1942, 2569
BmuI	ACTGGG	1 cut(s)	2230
BpiI	GAAGAC	1 cut(s)	69
BplI	GAGNNNNNCTC	4 cut(s)	1717, 1749, 2095, 2127
BpmI	CTGGAG	3 cut(s)	494, 674, 1565
Bpu10I	CCTNAGC	2 cut(s)	315, 2179
Bpu14I	TTCGAA	1 cut(s)	643
BpuEI	CTTGAG	2 cut(s)	832, 2072
BpuMI	CCSGG	3 cut(s)	127, 128, 1874
BsaAI	YACGTR	1 cut(s)	1559
BsaHI	GRCGYC	1 cut(s)	1399
BsaJI	CCNNGG	6 cut(s)	79, 126, 527, 1181, 1736, 1893
BsaXI	ACNNNNNCTCC	4 cut(s)	1816, 1846, 2343, 2373
Bsc4I	CCNNNNNNNGG	8 cut(s)	52, 697, 1286, 1791, 2495, 2496, 2597, 2598
Bse118I	RCCGGY	1 cut(s)	163
Bse1I	ACTGG	4 cut(s)	299, 1244, 1548, 2236
Bse3DI	GCAATG	5 cut(s)	150, 445, 1446, 2368, 2694
BseBI	CCWGG	5 cut(s)	59, 81, 528, 1307, 1970
BseDI	CCNNGG	6 cut(s)	79, 126, 527, 1181, 1736, 1893
BseGI	GGATG	2 cut(s)	460, 594
BseLI	CCNNNNNNNGG	8 cut(s)	52, 697, 1286, 1791, 2495, 2496, 2597, 2598
BseMI	GCAATG	5 cut(s)	150, 445, 1446, 2368, 2694
BseMII	CTCAG	8 cut(s)	306, 441, 973, 1230, 1507, 2076, 2437, 2606
BseNI	ACTGG	4 cut(s)	299, 1244, 1548, 2236
BseRI	GAGGAG	2 cut(s)	93, 1348
BseYI	CCCAGC	1 cut(s)	1988
BsgI	GTGCAG	2 cut(s)	294, 2136
BshFI	GGCC	5 cut(s)	167, 593, 656, 1741, 2107
BshNI	GGYRCC	1 cut(s)	1141
BsiHKAI	GWGCWC	4 cut(s)	1084, 1492, 2058, 2485
BsiHKCI	CYCGRG	3 cut(s)	95, 126, 2063
BsiSI	CCGG	4 cut(s)	127, 164, 1641, 1873
BslFI	GGGAC	2 cut(s)	544, 1889
BslI	CCNNNNNNNGG	8 cut(s)	52, 697, 1286, 1791, 2495, 2496, 2597, 2598
BsmAI	GTCTC	1 cut(s)	1629
BsmFI	GGGAC	2 cut(s)	544, 1889
BsmI	GAATGC	1 cut(s)	2611
BsnI	GGCC	5 cut(s)	167, 593, 656, 1741, 2107
BsoBI	CYCGRG	3 cut(s)	95, 126, 2063
Bsp119I	TTCGAA	1 cut(s)	643
Bsp1286I	GDGCHC	4 cut(s)	1084, 1492, 2058, 2485
BspACI	CCGC	5 cut(s)	241, 560, 2566, 2609, 2739
BspANI	GGCC	5 cut(s)	167, 593, 656, 1741, 2107
BspCNI	CTCAG	8 cut(s)	307, 442, 972, 1229, 1506, 2077, 2436, 2607
BspHI	TCATGA	1 cut(s)	810
BspLI	GGNNCC	5 cut(s)	78, 102, 689, 1143, 1469
BspMAI	CTGCAG	3 cut(s)	54, 877, 1778
BspMI	ACCTGC	2 cut(s)	43, 2458
BspPI	GGATC	6 cut(s)	78, 91, 835, 911, 1506, 1832
BspQI	GCTCTTC	1 cut(s)	1089
BspT104I	TTCGAA	1 cut(s)	643
BspT107I	GGYRCC	1 cut(s)	1141
BspTI	CTTAAG	1 cut(s)	2630
BsrDI	GCAATG	5 cut(s)	150, 445, 1446, 2368, 2694
BsrFI	RCCGGY	1 cut(s)	163
BsrI	ACTGG	4 cut(s)	299, 1244, 1548, 2236
BssAI	RCCGGY	1 cut(s)	163
BssECI	CCNNGG	6 cut(s)	79, 126, 527, 1181, 1736, 1893
BssNAI	GTATAC	1 cut(s)	2164
BssNI	GRCGYC	1 cut(s)	1399
BssT1I	CCWWGG	3 cut(s)	1181, 1736, 1893
Bst1107I	GTATAC	1 cut(s)	2164
Bst2UI	CCWGG	5 cut(s)	59, 81, 528, 1307, 1970
Bst4CI	ACNGT	7 cut(s)	118, 484, 600, 1929, 2226, 2282, 2719
Bst6I	CTCTTC	3 cut(s)	550, 1089, 2775
BstACI	GRCGYC	1 cut(s)	1399
BstAFI	CTTAAG	1 cut(s)	2630
BstBAI	YACGTR	1 cut(s)	1559
BstBI	TTCGAA	1 cut(s)	643
BstC8I	GCNNGC	4 cut(s)	33, 267, 2148, 2409
BstF5I	GGATG	2 cut(s)	460, 594
BstH2I	RGCGCY	2 cut(s)	473, 2147
BstHHI	GCGC	3 cut(s)	472, 2146, 2687
BstMAI	GTCTC	1 cut(s)	1629
BstMWI	GCNNNNNNNGC	8 cut(s)	28, 237, 713, 722, 964, 1773, 2150, 2682
BstNI	CCWGG	5 cut(s)	59, 81, 528, 1307, 1970
BstNSI	RCATGY	1 cut(s)	765
BstSCI	CCNGG	8 cut(s)	57, 79, 125, 126, 526, 1305, 1872, 1968
BstSFI	CTRYAG	3 cut(s)	50, 873, 1774
BstSNI	TACGTA	1 cut(s)	1559
BstV2I	GAAGAC	1 cut(s)	69
BstX2I	RGATCY	8 cut(s)	70, 83, 827, 903, 1498, 1837, 2508, 2762
BstXI	CCANNNNNNTGG	1 cut(s)	227
BstYI	RGATCY	8 cut(s)	70, 83, 827, 903, 1498, 1837, 2508, 2762
BstZ17I	GTATAC	1 cut(s)	2164
BsuRI	GGCC	5 cut(s)	167, 593, 656, 1741, 2107
BtgZI	GCGATG	2 cut(s)	1965, 2459
BtsCI	GGATG	2 cut(s)	460, 594
BtsI	GCAGTG	2 cut(s)	214, 2168
BtsIMutI	CAGTG	6 cut(s)	214, 420, 480, 1934, 2168, 2287
BveI	ACCTGC	2 cut(s)	43, 2458
Cac8I	GCNNGC	4 cut(s)	33, 267, 2148, 2409
CaiI	CAGNNNCTG	2 cut(s)	533, 563
CciI	TCATGA	1 cut(s)	810
CfoI	GCGC	3 cut(s)	472, 2146, 2687
Cfr10I	RCCGGY	1 cut(s)	163
Cfr13I	GGNCC	7 cut(s)	55, 76, 161, 196, 302, 916, 1754
Cfr9I	CCCGGG	1 cut(s)	126
CseI	GACGC	3 cut(s)	144, 297, 2036
Csp6I	GTAC	3 cut(s)	1142, 2257, 2720
CviAII	CATG	7 cut(s)	393, 762, 811, 1373, 1786, 2451, 2537
CviQI	GTAC	3 cut(s)	1142, 2257, 2720
DriI	GACNNNNNGTC	2 cut(s)	154, 376
EaeI	YGGCCR	1 cut(s)	591
Eam1104I	CTCTTC	3 cut(s)	550, 1089, 2775
Eam1105I	GACNNNNNGTC	2 cut(s)	154, 376
EarI	CTCTTC	3 cut(s)	550, 1089, 2775
EciI	GGCGGA	1 cut(s)	2754
Ecl136II	GAGCTC	1 cut(s)	1082
Eco105I	TACGTA	1 cut(s)	1559
Eco130I	CCWWGG	3 cut(s)	1181, 1736, 1893
Eco147I	AGGCCT	1 cut(s)	1741
Eco24I	GRGCYC	1 cut(s)	1084
Eco32I	GATATC	3 cut(s)	576, 1117, 2082
Eco47I	GGWCC	7 cut(s)	55, 76, 161, 196, 302, 916, 1754
Eco47III	AGCGCT	2 cut(s)	471, 2145
Eco53kI	GAGCTC	1 cut(s)	1082
Eco57I	CTGAAG	2 cut(s)	1092, 1734
Eco88I	CYCGRG	3 cut(s)	95, 126, 2063
EcoICRI	GAGCTC	1 cut(s)	1082
EcoO109I	RGGNCCY	2 cut(s)	302, 1754
EcoRI	GAATTC	3 cut(s)	487, 2431, 2455
EcoRII	CCWGG	5 cut(s)	57, 79, 526, 1305, 1968
EcoRV	GATATC	3 cut(s)	576, 1117, 2082
EcoT14I	CCWWGG	3 cut(s)	1181, 1736, 1893
EcoT22I	ATGCAT	1 cut(s)	1426
EcoT38I	GRGCYC	1 cut(s)	1084
ErhI	CCWWGG	3 cut(s)	1181, 1736, 1893
FaeI	CATG	7 cut(s)	396, 765, 814, 1376, 1789, 2454, 2540
FalI	AAGNNNNNCTT	2 cut(s)	1090, 1122
FaqI	GGGAC	2 cut(s)	544, 1889
FatI	CATG	7 cut(s)	392, 761, 810, 1372, 1785, 2450, 2536
FauI	CCCGC	1 cut(s)	2602
FbaI	TGATCA	2 cut(s)	442, 1074
FblI	GTMKAC	2 cut(s)	1276, 2163
FokI	GGATG	2 cut(s)	467, 601
FriOI	GRGCYC	1 cut(s)	1084
FspBI	CTAG	5 cut(s)	270, 611, 1188, 2363, 2402
GlaI	GCGC	3 cut(s)	471, 2145, 2686
GsaI	CCCAGC	1 cut(s)	1992
GsuI	CTGGAG	3 cut(s)	494, 674, 1565
HaeII	RGCGCY	2 cut(s)	473, 2147
HaeIII	GGCC	5 cut(s)	167, 593, 656, 1741, 2107
HapII	CCGG	4 cut(s)	127, 164, 1641, 1873
HgaI	GACGC	3 cut(s)	144, 297, 2036
HhaI	GCGC	3 cut(s)	472, 2146, 2687
Hin1I	GRCGYC	1 cut(s)	1399
Hin1II	CATG	7 cut(s)	396, 765, 814, 1376, 1789, 2454, 2540
Hin6I	GCGC	3 cut(s)	470, 2144, 2685
HinP1I	GCGC	3 cut(s)	470, 2144, 2685
HincII	GTYRAC	3 cut(s)	1211, 1277, 2026
HindII	GTYRAC	3 cut(s)	1211, 1277, 2026
HindIII	AAGCTT	1 cut(s)	2633
HpaII	CCGG	4 cut(s)	127, 164, 1641, 1873
HphI	GGTGA	5 cut(s)	106, 158, 407, 874, 2614
Hpy166II	GTNNAC	6 cut(s)	203, 823, 1211, 1277, 2026, 2164
Hpy8I	GTNNAC	6 cut(s)	203, 823, 1211, 1277, 2026, 2164
Hpy99I	CGWCG	2 cut(s)	313, 1278
HpyAV	CCTTC	9 cut(s)	178, 691, 731, 859, 1092, 1668, 1752, 2035, 2350
HpyCH4III	ACNGT	7 cut(s)	118, 484, 600, 1929, 2226, 2282, 2719
HpyCH4IV	ACGT	5 cut(s)	607, 1294, 1399, 1486, 1558
HpyF10VI	GCNNNNNNNGC	8 cut(s)	28, 237, 713, 722, 964, 1773, 2150, 2682
HpySE526I	ACGT	5 cut(s)	607, 1294, 1399, 1486, 1558
Hsp92I	GRCGYC	1 cut(s)	1399
Hsp92II	CATG	7 cut(s)	396, 765, 814, 1376, 1789, 2454, 2540
HspAI	GCGC	3 cut(s)	470, 2144, 2685
KpnI	GGTACC	1 cut(s)	1145
Ksp22I	TGATCA	2 cut(s)	442, 1074
LguI	GCTCTTC	1 cut(s)	1089
LmnI	GCTCC	7 cut(s)	13, 25, 106, 693, 1738, 1875, 2141
LweI	GCATC	6 cut(s)	788, 1411, 1433, 1891, 1942, 2569
MaeI	CTAG	5 cut(s)	270, 611, 1188, 2363, 2402
MaeII	ACGT	5 cut(s)	607, 1294, 1399, 1486, 1558
MflI	RGATCY	8 cut(s)	70, 83, 827, 903, 1498, 1837, 2508, 2762
MhlI	GDGCHC	4 cut(s)	1084, 1492, 2058, 2485
MlsI	TGGCCA	1 cut(s)	593
MluNI	TGGCCA	1 cut(s)	593
MlyI	GAGTC	5 cut(s)	386, 669, 1216, 1601, 1631
MmeI	TCCRAC	1 cut(s)	894
Mox20I	TGGCCA	1 cut(s)	593
Mph1103I	ATGCAT	1 cut(s)	1426
MroXI	GAANNNNTTC	1 cut(s)	2431
MscI	TGGCCA	1 cut(s)	593
MseI	TTAA	4 cut(s)	248, 1226, 1983, 2631
MslI	CAYNNNNRTG	2 cut(s)	1887, 2772
Msp20I	TGGCCA	1 cut(s)	593
MspA1I	CMGCKG	2 cut(s)	560, 2566
MspCI	CTTAAG	1 cut(s)	2630
MspI	CCGG	4 cut(s)	127, 164, 1641, 1873
MspR9I	CCNGG	8 cut(s)	59, 81, 127, 128, 528, 1307, 1874, 1970
Mva1269I	GAATGC	1 cut(s)	2611
MvaI	CCWGG	5 cut(s)	59, 81, 528, 1307, 1970
MwoI	GCNNNNNNNGC	8 cut(s)	28, 237, 713, 722, 964, 1773, 2150, 2682
NciI	CCSGG	3 cut(s)	127, 128, 1874
NlaIII	CATG	7 cut(s)	396, 765, 814, 1376, 1789, 2454, 2540
NlaIV	GGNNCC	5 cut(s)	78, 102, 689, 1143, 1469
NmeAIII	GCCGAG	2 cut(s)	956, 2504
NmuCI	GTSAC	7 cut(s)	118, 146, 478, 1769, 1788, 2006, 2276
NsiI	ATGCAT	1 cut(s)	1426
NspI	RCATGY	1 cut(s)	765
NspV	TTCGAA	1 cut(s)	643
PaeR7I	CTCGAG	2 cut(s)	95, 2063
PagI	TCATGA	1 cut(s)	810
PceI	AGGCCT	1 cut(s)	1741
PciI	ACATGT	1 cut(s)	761
PciSI	GCTCTTC	1 cut(s)	1089
PctI	GAATGC	1 cut(s)	2611
PdmI	GAANNNNTTC	1 cut(s)	2431
PfeI	GAWTC	9 cut(s)	1043, 1175, 1259, 1509, 1549, 1712, 2115, 2184, 2231
PflFI	GACNNNGTC	1 cut(s)	920
PfoI	TCCNGGA	2 cut(s)	57, 1872
PleI	GAGTC	5 cut(s)	385, 668, 1215, 1600, 1630
PpsI	GAGTC	5 cut(s)	385, 668, 1215, 1600, 1630
Ppu21I	YACGTR	1 cut(s)	1559
PpuMI	RGGWCCY	2 cut(s)	302, 1754
PscI	ACATGT	1 cut(s)	761
PsiI	TTATAA	1 cut(s)	569
Psp124BI	GAGCTC	1 cut(s)	1084
Psp5II	RGGWCCY	2 cut(s)	302, 1754
Psp6I	CCWGG	5 cut(s)	57, 79, 526, 1305, 1968
PspFI	CCCAGC	1 cut(s)	1988
PspGI	CCWGG	5 cut(s)	57, 79, 526, 1305, 1968
PspN4I	GGNNCC	5 cut(s)	78, 102, 689, 1143, 1469
PspPI	GGNCC	7 cut(s)	55, 76, 161, 196, 302, 916, 1754
PspPPI	RGGWCCY	2 cut(s)	302, 1754
PspXI	VCTCGAGB	1 cut(s)	95
PstI	CTGCAG	3 cut(s)	54, 877, 1778
PstNI	CAGNNNCTG	2 cut(s)	533, 563
PsuI	RGATCY	8 cut(s)	70, 83, 827, 903, 1498, 1837, 2508, 2762
PsyI	GACNNNGTC	1 cut(s)	920
RsaI	GTAC	3 cut(s)	1143, 2258, 2721
RsaNI	GTAC	3 cut(s)	1142, 2257, 2720
RseI	CAYNNNNRTG	2 cut(s)	1887, 2772
SacI	GAGCTC	1 cut(s)	1084
SalI	GTCGAC	1 cut(s)	1275
SapI	GCTCTTC	1 cut(s)	1089
SaqAI	TTAA	4 cut(s)	248, 1226, 1983, 2631
Sau96I	GGNCC	7 cut(s)	55, 76, 161, 196, 302, 916, 1754
ScaI	AGTACT	1 cut(s)	2721
SchI	GAGTC	5 cut(s)	386, 669, 1216, 1601, 1631
ScrFI	CCNGG	8 cut(s)	59, 81, 127, 128, 528, 1307, 1874, 1970
SduI	GDGCHC	4 cut(s)	1084, 1492, 2058, 2485
SfaNI	GCATC	6 cut(s)	788, 1411, 1433, 1891, 1942, 2569
SfcI	CTRYAG	3 cut(s)	50, 873, 1774
Sfr274I	CTCGAG	2 cut(s)	95, 2063
SfuI	TTCGAA	1 cut(s)	643
SinI	GGWCC	7 cut(s)	55, 76, 161, 196, 302, 916, 1754
SlaI	CTCGAG	2 cut(s)	95, 2063
SmaI	CCCGGG	1 cut(s)	128
SmiMI	CAYNNNNRTG	2 cut(s)	1887, 2772
SmlI	CTYRAG	5 cut(s)	95, 847, 2051, 2063, 2630
SmoI	CTYRAG	5 cut(s)	95, 847, 2051, 2063, 2630
SnaBI	TACGTA	1 cut(s)	1559
SpeI	ACTAGT	1 cut(s)	2401
SseBI	AGGCCT	1 cut(s)	1741
SsiI	CCGC	5 cut(s)	241, 560, 2566, 2609, 2739
SspI	AATATT	1 cut(s)	1573
SspMI	CTAG	5 cut(s)	270, 611, 1188, 2363, 2402
SstI	GAGCTC	1 cut(s)	1084
StuI	AGGCCT	1 cut(s)	1741
StyD4I	CCNGG	8 cut(s)	57, 79, 125, 126, 526, 1305, 1872, 1968
StyI	CCWWGG	3 cut(s)	1181, 1736, 1893
TaaI	ACNGT	7 cut(s)	118, 484, 600, 1929, 2226, 2282, 2719
TaiI	ACGT	5 cut(s)	610, 1297, 1402, 1489, 1561
TaqI	TCGA	8 cut(s)	96, 380, 584, 643, 1276, 2064, 2113, 2734
TatI	WGTACW	2 cut(s)	2256, 2719
TauI	GCSGC	2 cut(s)	243, 563
TfiI	GAWTC	9 cut(s)	1043, 1175, 1259, 1509, 1549, 1712, 2115, 2184, 2231
Tru1I	TTAA	4 cut(s)	248, 1226, 1983, 2631
Tru9I	TTAA	4 cut(s)	248, 1226, 1983, 2631
TscAI	CASTG	6 cut(s)	214, 427, 487, 1934, 2175, 2287
TseFI	GTSAC	7 cut(s)	118, 146, 478, 1769, 1788, 2006, 2276
Tsp45I	GTSAC	7 cut(s)	118, 146, 478, 1769, 1788, 2006, 2276
TspGWI	ACGGA	6 cut(s)	188, 840, 1395, 1460, 1821, 1835
TspMI	CCCGGG	1 cut(s)	126
TspRI	CASTG	6 cut(s)	214, 427, 487, 1934, 2175, 2287
Tth111I	GACNNNGTC	1 cut(s)	920
Vha464I	CTTAAG	1 cut(s)	2630
VpaK11BI	GGWCC	7 cut(s)	55, 76, 161, 196, 302, 916, 1754
XapI	RAATTY	7 cut(s)	487, 620, 1311, 2070, 2431, 2455, 2518
XceI	RCATGY	1 cut(s)	765
XhoI	CTCGAG	2 cut(s)	95, 2063
XmaI	CCCGGG	1 cut(s)	126
XmiI	GTMKAC	2 cut(s)	1276, 2163
XmnI	GAANNNNTTC	1 cut(s)	2431
XspI	CTAG	5 cut(s)	270, 611, 1188, 2363, 2402
ZraI	GACGTC	1 cut(s)	1400
ZrmI	AGTACT	1 cut(s)	2721
Zsp2I	ATGCAT	1 cut(s)	1426

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.