Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0003250

Physical Location & Seq

Genomic Coordinates Forward (+)

59299287 .. 59305135

Gene Structure 5849 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00265920.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2538 bp

ATGGCAAAACAGGAGCAAAAGCAGGAGCAGCCTGCAATCATCAACCCTCTGCAGGTCCAGGAAGACGGGGATCTGGACCCTGGATCTGACGACCTCGAGGCTCCTCTGCCTCTCACCGTCACTTCCCGGGTCTTGTATATGTTGGGTGACATTGCGTCTGGACCGGCCTTCAAAATCACTCAATGGCTGGAATTGGTCCGTAAACGCAGTGCCAAATACCATTCCTCTGGCTTCCCTCGCCGCCCATTAAGGCTTGACACAATGCCTGCTAGTGCAGGAGAGTGTAGTGAGGACTGGAAAGGTCCTGCGTCGCCTGAGCAAACCACAGAAGTCAATCTCTGGGAAAGACTTGGTCACCGTGAATTCGGGGGAGTTGTTTTCTTTGCCCTTTTCAACCAGCCAGGGACTGATGATAACTCTTCTAAGGAAGCAGCGGCTGTTATTAAGATATCCTCATCGAGGATGGCCACACAGTCTGAACGTCTAGGTTACGAATTTGCAAAGTGGCTTGGAGTTCGAACTCCACAGGCCAGAGTCATACATAATTGTAGTATGGAGTGGCTCCAGATGAAGGAAGCAGCAGAAAAAGCAAGGGAAGCAGCAGTTTCAGAAGGAGATGAATGTGGGGAAATGACATGTTCGGAACTTTTGGAAGCTCTTGAACTTAGCCGATGCCTTTTGCTCATGAGCTATGTTCACGGATCTCCTTTACTTGAAAGCTCAAGTGTATTTGAGGTGAAGGAAACTGCAGAGAAAACAGCAGCTTCTCTTGGTAGGATCTTGATGTTGGACCTTGTCATCAGAAATGAAGATAGACTACCTTGCCGTCAGCTCAGATGGCGTGGAAACTCGGCAAATCTATTGTTTGCAGACAAAATGGATTTTGGAAATATGGACAGATTGGAGGAAGCATTTGATTCTGCCATCAAGAGATACAAACCAAGAGTGATCAGAGCTCTTCAAAAGGATAGAAGGGCAACTTCAGTAGATATCAGATTGCATAATACAGCATTGGTACCACAGAAATCTGATCTTTCTGACATTATGGATTCGCCAAGGTCTAGTCAGATGAGTGTAAAGAGTCAACTCTCAGATGATTTAATGTTTTCCGATTTTCCAGTTGTGGCTATTGATTCTGGCGTTCCTCGTCGACCTCCTGCTGGAAAACGTGCAAATGACCAGGAAATCTATCCAAAGTTGGTTGAGTTACTACTGAACAATTCTGAATACTCCTCTAATCTGTTACATGACATAACATTAGGGAAATTAGGACGTCCTCCGTTACAAGATACTGATGCATTTGATACACAAGCAATGGAAATGACTTCGGTTGTTCAAGGGGAGTCCGATAAAGAGGATTTGGTAGTTACTGAGTCTCCATCACAAGCTGCCGGAAGTGGTTATTTTCCTTCTACACCAAGTAAGGAGAGGCTTGTCAATGAGAACCATTCAGATTTTAGTGATTCCGAATTGCAGAGAACTGCTCCAAGGCCTTCAACTTCAGGACTTAAAGAAAGCTGTGACTGCAGCTCTCCTATGTCACGGGAGAGTTGGCACGGAAAGTTTTGGAAAGGGAGTGCAAGTTCAGATCCCCTCCGTAGTCTGCGTTTGACAGCAAAGCTCCGGGACTTTCATAAAAATGCCAAGGTCGATGCTGAAACGAACAAAGAATTGGAACAGTGGAATGAAATGCTAAAGAGCGATGCAGTCAAACTTTGCCAGGAGAACAATTTTAATGCTGGGTTCTTTGAGGGTAGTGACAATAACGGCGTAGTTGACGCTTATGAATTGAAGGTCAGACTTGAGCACATTCTCGAGAGAATTTCATTGATATCTGAGGCTGCAAATACAGAGAGGCCATCTCGAATCACAAGTAGCCTGTTTATTGGTGGAGCGCTTGCTGCACGATCTGTATACACACTGCAACGCTTAGGAATCACTCATATATTATGTTTGTGTTCCAATGAAATTGGACAGTCAGATTCTCAGTTTCCTGATCTATTTGAGTACAAAAACTTCTCTATATGTGACAGTGAAGATTTCAACATCAGCAACATCTTTGATGAAGCTGTTGAGTTTATTGATCGTGTTGAAGAAACAGGAGGGAAGGTTCTAGTCCATTGCTTTGAGGGAAAAAGTAGAAGTGCTACACTAGTGCTTGCATACCTAATGCTCAGAAAGAATTCCACTCTATTAGAAGCATGGAATTCTCTAAAGCAGGTTCATCGCCGAGCACAACCCAACGATGGGTTTGCGAAGATCTTGTTGAATTTGGATAAGAAACTGCATGGTAAAGTTTCAATGGAGTGGCATCAGCGGAAGCCAATGATGAAAGTTTGCCCCATCTGTGGGGTGAATGCAGGTCTGAGTAGCAGCTCTCTTAAGCTTCATTTACAGAAATCACATAAGAAACTATCGTCTGGTAGCGTGGATAGCGCAATGACAATGGAAATACAAAAGGCTTTGACAGTACTAAAAATCAGTCGAGGTGGAAGTGTCAGCCCCACACAAAGATCTCATTCAGATGTTGAAGAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000226 GO:0001101 GO:0003674 GO:0003824 GO:0004721 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007010 GO:0007017 GO:0008138 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009987 GO:0010033 GO:0010119 GO:0010468 GO:0016043 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019222 GO:0019538 GO:0030865 GO:0031122 GO:0033993 GO:0036211 GO:0042221 GO:0042578 GO:0043170 GO:0043412 GO:0043622 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0060255 GO:0065007 GO:0071704 GO:0071840 GO:0097305 GO:0097435 GO:0140096 GO:1901564 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Act-Frag_cataly DSPc

Protein Analysis

845

Amino Acids

94.23

Weight (kDa)

5.78

Isoelectric Point (pI)

52.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Act-Frag_cataly	PF09192	124 - 381	1.8e-38	Actin-fragmin kinase, catalytic
DSPc	PF00782	629 - 759	1.1e-24	Dual specificity phosphatase, catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0003250)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G23720 AT5G23720 AT5G23720
fragaria_vesca	FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220
malus_domestica	MD06G1132400.v1.1 MD14G1148800.v1.1
prunus_persica	Prupe.5G143400_v2.0.a1
pyrus_communis	pycom06g12400 pycom14g12360
rosa_chinensis	RchiOBHm_Chr4g0416471 RchiOBHm_Chr7g0188941 RchiOBHm_Chr7g0194331
rosa_laevigata	RLG00000004049 RLG00000004648
rosa_multiflora	Rmu_co8071116.1_g000001 Rmu_sc0007831.1_g000001 Rmu_sc0032883.1_g000001 Rmu_ssc0000113.1_g000006
rosa_roxburghii	Rroxscaffold_3G00265920
rosa_rugosa	Rorug06G0494800 Rorug06G0494900 Rorug06G0495000
rosa_samantha	Rh7AG099700 Rh7BG101800 Rh7CG103100 Rh7CG146600 Rh7DG101600 Rh7DG144200
rosa_wichuraiana	Rw7G008590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1276
AbsI	CCTCGAGG	1 cut(s)	95
Acc36I	ACCTGC	3 cut(s)	43, 2209, 2351
Acc65I	GGTACC	1 cut(s)	1015
AccB1I	GGYRCC	1 cut(s)	1015
AccI	GTMKAC	2 cut(s)	1150, 1914
AciI	CCGC	3 cut(s)	241, 434, 2317
AclWI	GGATC	5 cut(s)	78, 91, 709, 785, 1583
AcoI	YGGCCR	1 cut(s)	465
AcsI	RAATTY	6 cut(s)	362, 494, 1821, 2182, 2206, 2269
AcuI	CTGAAG	2 cut(s)	966, 1485
AcyI	GRCGYC	1 cut(s)	1273
AfaI	GTAC	3 cut(s)	1017, 2009, 2472
AfeI	AGCGCT	1 cut(s)	1896
AfiI	CCNNNNNNNGG	8 cut(s)	52, 459, 571, 1160, 2246, 2247, 2348, 2349
AflII	CTTAAG	1 cut(s)	2381
AflIII	ACRYGT	1 cut(s)	635
AhdI	GACNNNNNGTC	1 cut(s)	154
AhlI	ACTAGT	1 cut(s)	2152
AjnI	CCWGG	5 cut(s)	57, 79, 400, 1179, 1719
AjuI	GAANNNNNNNTTGG	2 cut(s)	1655, 1687
AloI	GAACNNNNNNTCC	4 cut(s)	1567, 1599, 2094, 2126
Alw21I	GWGCWC	3 cut(s)	958, 1809, 2236
Alw26I	GTCTC	1 cut(s)	1380
AlwI	GGATC	5 cut(s)	78, 91, 709, 785, 1583
AlwNI	CAGNNNCTG	2 cut(s)	407, 437
Ama87I	CYCGRG	3 cut(s)	95, 126, 1814
Aor51HI	AGCGCT	1 cut(s)	1896
AoxI	GGCC	5 cut(s)	165, 465, 528, 1490, 1856
ApoI	RAATTY	6 cut(s)	362, 494, 1821, 2182, 2206, 2269
Asp700I	GAANNNNTTC	1 cut(s)	2182
Asp718I	GGTACC	1 cut(s)	1015
AspLEI	GCGC	2 cut(s)	1897, 2438
AspS9I	GGNCC	6 cut(s)	55, 76, 161, 196, 302, 790
AsuC2I	CCSGG	3 cut(s)	127, 128, 1625
AsuHPI	GGTGA	5 cut(s)	106, 158, 347, 748, 2365
AsuII	TTCGAA	1 cut(s)	517
AvaI	CYCGRG	3 cut(s)	95, 126, 1814
AvaII	GGWCC	6 cut(s)	55, 76, 161, 196, 302, 790
BalI	TGGCCA	1 cut(s)	467
BanI	GGYRCC	1 cut(s)	1015
BanII	GRGCYC	1 cut(s)	958
BarI	GAAGNNNNNNTAC	4 cut(s)	801, 833, 2000, 2032
BbsI	GAAGAC	1 cut(s)	69
Bbv12I	GWGCWC	3 cut(s)	958, 1809, 2236
BccI	CCATC	7 cut(s)	457, 831, 932, 1387, 1867, 2240, 2351
BceAI	ACGGC	2 cut(s)	810, 1783
BcgI	CGANNNNNNTGC	4 cut(s)	2207, 2234, 2241, 2268
BciT130I	CCWGG	5 cut(s)	59, 81, 402, 1181, 1721
BclI	TGATCA	1 cut(s)	948
BcnI	CCSGG	3 cut(s)	127, 128, 1625
BcoDI	GTCTC	1 cut(s)	1380
BcuI	ACTAGT	1 cut(s)	2152
BfaI	CTAG	5 cut(s)	270, 485, 1062, 2114, 2153
BfmI	CTRYAG	3 cut(s)	50, 747, 1525
BfoI	RGCGCY	1 cut(s)	1898
BfrI	CTTAAG	1 cut(s)	2381
BfuAI	ACCTGC	3 cut(s)	43, 2209, 2351
BglII	AGATCT	2 cut(s)	2259, 2513
BmcAI	AGTACT	1 cut(s)	2472
Bme1390I	CCNGG	8 cut(s)	59, 81, 127, 128, 402, 1181, 1625, 1721
Bme18I	GGWCC	6 cut(s)	55, 76, 161, 196, 302, 790
BmeRI	GACNNNNNGTC	1 cut(s)	154
BmeT110I	CYCGRG	3 cut(s)	95, 126, 1814
BmgT120I	GGNCC	6 cut(s)	55, 76, 161, 196, 302, 790
BmiI	GGNNCC	4 cut(s)	78, 102, 563, 1017
BmrFI	CCNGG	8 cut(s)	59, 81, 127, 128, 402, 1181, 1625, 1721
BmsI	GCATC	5 cut(s)	662, 1285, 1642, 1693, 2320
BpiI	GAAGAC	1 cut(s)	69
BplI	GAGNNNNNCTC	4 cut(s)	1468, 1500, 1846, 1878
BpmI	CTGGAG	1 cut(s)	548
Bpu10I	CCTNAGC	2 cut(s)	315, 1930
Bpu14I	TTCGAA	1 cut(s)	517
BpuEI	CTTGAG	2 cut(s)	706, 1823
BpuMI	CCSGG	3 cut(s)	127, 128, 1625
BsaHI	GRCGYC	1 cut(s)	1273
BsaJI	CCNNGG	6 cut(s)	79, 126, 401, 1055, 1487, 1644
BsaXI	ACNNNNNCTCC	6 cut(s)	1360, 1390, 1567, 1597, 2094, 2124
Bsc4I	CCNNNNNNNGG	8 cut(s)	52, 459, 571, 1160, 2246, 2247, 2348, 2349
Bse118I	RCCGGY	1 cut(s)	163
Bse1I	ACTGG	2 cut(s)	299, 1118
Bse3DI	GCAATG	4 cut(s)	150, 1320, 2119, 2445
BseBI	CCWGG	5 cut(s)	59, 81, 402, 1181, 1721
BseDI	CCNNGG	6 cut(s)	79, 126, 401, 1055, 1487, 1644
BseGI	GGATG	1 cut(s)	468
BseLI	CCNNNNNNNGG	8 cut(s)	52, 459, 571, 1160, 2246, 2247, 2348, 2349
BseMI	GCAATG	4 cut(s)	150, 1320, 2119, 2445
BseMII	CTCAG	8 cut(s)	306, 847, 1104, 1362, 1827, 2000, 2188, 2357
BseNI	ACTGG	2 cut(s)	299, 1118
BseRI	GAGGAG	2 cut(s)	93, 1222
BseYI	CCCAGC	1 cut(s)	1739
BsgI	GTGCAG	2 cut(s)	294, 1887
BshFI	GGCC	5 cut(s)	167, 467, 530, 1492, 1858
BshNI	GGYRCC	1 cut(s)	1015
BsiHKAI	GWGCWC	3 cut(s)	958, 1809, 2236
BsiHKCI	CYCGRG	3 cut(s)	95, 126, 1814
BsiSI	CCGG	4 cut(s)	127, 164, 1392, 1624
BslFI	GGGAC	2 cut(s)	418, 1640
BslI	CCNNNNNNNGG	8 cut(s)	52, 459, 571, 1160, 2246, 2247, 2348, 2349
BsmAI	GTCTC	1 cut(s)	1380
BsmFI	GGGAC	2 cut(s)	418, 1640
BsmI	GAATGC	1 cut(s)	2362
BsnI	GGCC	5 cut(s)	167, 467, 530, 1492, 1858
BsoBI	CYCGRG	3 cut(s)	95, 126, 1814
Bsp119I	TTCGAA	1 cut(s)	517
Bsp1286I	GDGCHC	3 cut(s)	958, 1809, 2236
BspACI	CCGC	3 cut(s)	241, 434, 2317
BspANI	GGCC	5 cut(s)	167, 467, 530, 1492, 1858
BspCNI	CTCAG	8 cut(s)	307, 846, 1103, 1363, 1828, 1999, 2187, 2358
BspHI	TCATGA	1 cut(s)	684
BspLI	GGNNCC	4 cut(s)	78, 102, 563, 1017
BspMAI	CTGCAG	3 cut(s)	54, 751, 1529
BspMI	ACCTGC	3 cut(s)	43, 2209, 2351
BspPI	GGATC	5 cut(s)	78, 91, 709, 785, 1583
BspQI	GCTCTTC	1 cut(s)	963
BspT104I	TTCGAA	1 cut(s)	517
BspT107I	GGYRCC	1 cut(s)	1015
BspTI	CTTAAG	1 cut(s)	2381
BsrDI	GCAATG	4 cut(s)	150, 1320, 2119, 2445
BsrFI	RCCGGY	1 cut(s)	163
BsrI	ACTGG	2 cut(s)	299, 1118
BssAI	RCCGGY	1 cut(s)	163
BssECI	CCNNGG	6 cut(s)	79, 126, 401, 1055, 1487, 1644
BssNAI	GTATAC	1 cut(s)	1915
BssNI	GRCGYC	1 cut(s)	1273
BssT1I	CCWWGG	3 cut(s)	1055, 1487, 1644
Bst1107I	GTATAC	1 cut(s)	1915
Bst2UI	CCWGG	5 cut(s)	59, 81, 402, 1181, 1721
Bst4CI	ACNGT	7 cut(s)	118, 359, 474, 1680, 1977, 2033, 2470
Bst6I	CTCTTC	3 cut(s)	424, 963, 2526
BstACI	GRCGYC	1 cut(s)	1273
BstAFI	CTTAAG	1 cut(s)	2381
BstBI	TTCGAA	1 cut(s)	517
BstC8I	GCNNGC	4 cut(s)	33, 267, 1899, 2160
BstEII	GGTNACC	1 cut(s)	353
BstENI	CCTNNNNNAGG	1 cut(s)	457
BstF5I	GGATG	1 cut(s)	468
BstH2I	RGCGCY	1 cut(s)	1898
BstHHI	GCGC	2 cut(s)	1897, 2438
BstMAI	GTCTC	1 cut(s)	1380
BstMWI	GCNNNNNNNGC	8 cut(s)	28, 237, 587, 596, 838, 1524, 1901, 2433
BstNI	CCWGG	5 cut(s)	59, 81, 402, 1181, 1721
BstNSI	RCATGY	1 cut(s)	639
BstPI	GGTNACC	1 cut(s)	353
BstSCI	CCNGG	8 cut(s)	57, 79, 125, 126, 400, 1179, 1623, 1719
BstSFI	CTRYAG	3 cut(s)	50, 747, 1525
BstV2I	GAAGAC	1 cut(s)	69
BstX2I	RGATCY	7 cut(s)	70, 83, 701, 777, 1588, 2259, 2513
BstXI	CCANNNNNNTGG	1 cut(s)	227
BstYI	RGATCY	7 cut(s)	70, 83, 701, 777, 1588, 2259, 2513
BstZ17I	GTATAC	1 cut(s)	1915
BsuRI	GGCC	5 cut(s)	167, 467, 530, 1492, 1858
BtgZI	GCGATG	2 cut(s)	1716, 2210
BtsCI	GGATG	1 cut(s)	468
BtsI	GCAGTG	2 cut(s)	214, 1919
BtsIMutI	CAGTG	4 cut(s)	214, 1685, 1919, 2038
BveI	ACCTGC	3 cut(s)	43, 2209, 2351
Cac8I	GCNNGC	4 cut(s)	33, 267, 1899, 2160
CaiI	CAGNNNCTG	2 cut(s)	407, 437
CciI	TCATGA	1 cut(s)	684
CfoI	GCGC	2 cut(s)	1897, 2438
Cfr10I	RCCGGY	1 cut(s)	163
Cfr13I	GGNCC	6 cut(s)	55, 76, 161, 196, 302, 790
Cfr9I	CCCGGG	1 cut(s)	126
CseI	GACGC	3 cut(s)	144, 297, 1787
Csp6I	GTAC	3 cut(s)	1016, 2008, 2471
CviAII	CATG	5 cut(s)	636, 685, 1247, 2202, 2288
CviQI	GTAC	3 cut(s)	1016, 2008, 2471
DriI	GACNNNNNGTC	1 cut(s)	154
EaeI	YGGCCR	1 cut(s)	465
Eam1104I	CTCTTC	3 cut(s)	424, 963, 2526
Eam1105I	GACNNNNNGTC	1 cut(s)	154
EarI	CTCTTC	3 cut(s)	424, 963, 2526
Ecl136II	GAGCTC	1 cut(s)	956
Eco130I	CCWWGG	3 cut(s)	1055, 1487, 1644
Eco147I	AGGCCT	1 cut(s)	1492
Eco24I	GRGCYC	1 cut(s)	958
Eco32I	GATATC	3 cut(s)	450, 991, 1833
Eco47I	GGWCC	6 cut(s)	55, 76, 161, 196, 302, 790
Eco47III	AGCGCT	1 cut(s)	1896
Eco53kI	GAGCTC	1 cut(s)	956
Eco57I	CTGAAG	2 cut(s)	966, 1485
Eco88I	CYCGRG	3 cut(s)	95, 126, 1814
Eco91I	GGTNACC	1 cut(s)	353
EcoICRI	GAGCTC	1 cut(s)	956
EcoNI	CCTNNNNNAGG	1 cut(s)	457
EcoO109I	RGGNCCY	1 cut(s)	302
EcoO65I	GGTNACC	1 cut(s)	353
EcoRI	GAATTC	3 cut(s)	362, 2182, 2206
EcoRII	CCWGG	5 cut(s)	57, 79, 400, 1179, 1719
EcoRV	GATATC	3 cut(s)	450, 991, 1833
EcoT14I	CCWWGG	3 cut(s)	1055, 1487, 1644
EcoT22I	ATGCAT	1 cut(s)	1300
EcoT38I	GRGCYC	1 cut(s)	958
ErhI	CCWWGG	3 cut(s)	1055, 1487, 1644
FaeI	CATG	5 cut(s)	639, 688, 1250, 2205, 2291
FalI	AAGNNNNNCTT	2 cut(s)	964, 996
FaqI	GGGAC	2 cut(s)	418, 1640
FatI	CATG	5 cut(s)	635, 684, 1246, 2201, 2287
FbaI	TGATCA	1 cut(s)	948
FblI	GTMKAC	2 cut(s)	1150, 1914
FokI	GGATG	1 cut(s)	475
FriOI	GRGCYC	1 cut(s)	958
FspBI	CTAG	5 cut(s)	270, 485, 1062, 2114, 2153
GlaI	GCGC	2 cut(s)	1896, 2437
GsaI	CCCAGC	1 cut(s)	1743
GsuI	CTGGAG	1 cut(s)	548
HaeII	RGCGCY	1 cut(s)	1898
HaeIII	GGCC	5 cut(s)	167, 467, 530, 1492, 1858
HapII	CCGG	4 cut(s)	127, 164, 1392, 1624
HgaI	GACGC	3 cut(s)	144, 297, 1787
HhaI	GCGC	2 cut(s)	1897, 2438
Hin1I	GRCGYC	1 cut(s)	1273
Hin1II	CATG	5 cut(s)	639, 688, 1250, 2205, 2291
Hin6I	GCGC	2 cut(s)	1895, 2436
HinP1I	GCGC	2 cut(s)	1895, 2436
HincII	GTYRAC	3 cut(s)	1085, 1151, 1777
HindII	GTYRAC	3 cut(s)	1085, 1151, 1777
HindIII	AAGCTT	1 cut(s)	2384
HpaII	CCGG	4 cut(s)	127, 164, 1392, 1624
HphI	GGTGA	5 cut(s)	106, 158, 347, 748, 2365
Hpy166II	GTNNAC	6 cut(s)	203, 697, 1085, 1151, 1777, 1915
Hpy8I	GTNNAC	6 cut(s)	203, 697, 1085, 1151, 1777, 1915
Hpy99I	CGWCG	2 cut(s)	313, 1152
HpyAV	CCTTC	9 cut(s)	178, 565, 605, 733, 966, 1419, 1503, 1786, 2101
HpyCH4III	ACNGT	7 cut(s)	118, 359, 474, 1680, 1977, 2033, 2470
HpyCH4IV	ACGT	3 cut(s)	481, 1168, 1273
HpyF10VI	GCNNNNNNNGC	8 cut(s)	28, 237, 587, 596, 838, 1524, 1901, 2433
HpySE526I	ACGT	3 cut(s)	481, 1168, 1273
Hsp92I	GRCGYC	1 cut(s)	1273
Hsp92II	CATG	5 cut(s)	639, 688, 1250, 2205, 2291
HspAI	GCGC	2 cut(s)	1895, 2436
KpnI	GGTACC	1 cut(s)	1019
Ksp22I	TGATCA	1 cut(s)	948
LguI	GCTCTTC	1 cut(s)	963
LmnI	GCTCC	7 cut(s)	13, 25, 106, 567, 1489, 1626, 1892
LweI	GCATC	5 cut(s)	662, 1285, 1642, 1693, 2320
MaeI	CTAG	5 cut(s)	270, 485, 1062, 2114, 2153
MaeII	ACGT	3 cut(s)	481, 1168, 1273
MboII	GAAGA	7 cut(s)	74, 411, 821, 950, 2048, 2105, 2269
MflI	RGATCY	7 cut(s)	70, 83, 701, 777, 1588, 2259, 2513
MhlI	GDGCHC	3 cut(s)	958, 1809, 2236
MlsI	TGGCCA	1 cut(s)	467
MluNI	TGGCCA	1 cut(s)	467
MlyI	GAGTC	4 cut(s)	543, 1090, 1352, 1382
MmeI	TCCRAC	1 cut(s)	768
Mox20I	TGGCCA	1 cut(s)	467
Mph1103I	ATGCAT	1 cut(s)	1300
MroXI	GAANNNNTTC	1 cut(s)	2182
MscI	TGGCCA	1 cut(s)	467
MseI	TTAA	6 cut(s)	248, 444, 1100, 1509, 1734, 2382
MslI	CAYNNNNRTG	2 cut(s)	1638, 2523
Msp20I	TGGCCA	1 cut(s)	467
MspA1I	CMGCKG	2 cut(s)	434, 2317
MspCI	CTTAAG	1 cut(s)	2381
MspI	CCGG	4 cut(s)	127, 164, 1392, 1624
MspR9I	CCNGG	8 cut(s)	59, 81, 127, 128, 402, 1181, 1625, 1721
Mva1269I	GAATGC	1 cut(s)	2362
MvaI	CCWGG	5 cut(s)	59, 81, 402, 1181, 1721
MwoI	GCNNNNNNNGC	8 cut(s)	28, 237, 587, 596, 838, 1524, 1901, 2433
NciI	CCSGG	3 cut(s)	127, 128, 1625
NlaIII	CATG	5 cut(s)	639, 688, 1250, 2205, 2291
NlaIV	GGNNCC	4 cut(s)	78, 102, 563, 1017
NmeAIII	GCCGAG	2 cut(s)	830, 2255
NmuCI	GTSAC	7 cut(s)	118, 146, 353, 1520, 1539, 1757, 2027
NsiI	ATGCAT	1 cut(s)	1300
NspI	RCATGY	1 cut(s)	639
NspV	TTCGAA	1 cut(s)	517
PaeR7I	CTCGAG	2 cut(s)	95, 1814
PagI	TCATGA	1 cut(s)	684
PceI	AGGCCT	1 cut(s)	1492
PciI	ACATGT	1 cut(s)	635
PciSI	GCTCTTC	1 cut(s)	963
PctI	GAATGC	1 cut(s)	2362
PdmI	GAANNNNTTC	1 cut(s)	2182
PfeI	GAWTC	7 cut(s)	917, 1049, 1133, 1463, 1866, 1935, 1982
PflFI	GACNNNGTC	2 cut(s)	351, 794
PfoI	TCCNGGA	2 cut(s)	57, 1623
PleI	GAGTC	4 cut(s)	542, 1089, 1351, 1381
PpsI	GAGTC	4 cut(s)	542, 1089, 1351, 1381
PpuMI	RGGWCCY	1 cut(s)	302
PscI	ACATGT	1 cut(s)	635
Psp124BI	GAGCTC	1 cut(s)	958
Psp5II	RGGWCCY	1 cut(s)	302
Psp6I	CCWGG	5 cut(s)	57, 79, 400, 1179, 1719
PspEI	GGTNACC	1 cut(s)	353
PspFI	CCCAGC	1 cut(s)	1739
PspGI	CCWGG	5 cut(s)	57, 79, 400, 1179, 1719
PspN4I	GGNNCC	4 cut(s)	78, 102, 563, 1017
PspPI	GGNCC	6 cut(s)	55, 76, 161, 196, 302, 790
PspPPI	RGGWCCY	1 cut(s)	302
PspXI	VCTCGAGB	1 cut(s)	95
PstI	CTGCAG	3 cut(s)	54, 751, 1529
PstNI	CAGNNNCTG	2 cut(s)	407, 437
PsuI	RGATCY	7 cut(s)	70, 83, 701, 777, 1588, 2259, 2513
PsyI	GACNNNGTC	2 cut(s)	351, 794
RsaI	GTAC	3 cut(s)	1017, 2009, 2472
RsaNI	GTAC	3 cut(s)	1016, 2008, 2471
RseI	CAYNNNNRTG	2 cut(s)	1638, 2523
SacI	GAGCTC	1 cut(s)	958
SalI	GTCGAC	1 cut(s)	1149
SapI	GCTCTTC	1 cut(s)	963
SaqAI	TTAA	6 cut(s)	248, 444, 1100, 1509, 1734, 2382
Sau96I	GGNCC	6 cut(s)	55, 76, 161, 196, 302, 790
ScaI	AGTACT	1 cut(s)	2472
SchI	GAGTC	4 cut(s)	543, 1090, 1352, 1382
ScrFI	CCNGG	8 cut(s)	59, 81, 127, 128, 402, 1181, 1625, 1721
SduI	GDGCHC	3 cut(s)	958, 1809, 2236
SfaNI	GCATC	5 cut(s)	662, 1285, 1642, 1693, 2320
SfcI	CTRYAG	3 cut(s)	50, 747, 1525
Sfr274I	CTCGAG	2 cut(s)	95, 1814
SfuI	TTCGAA	1 cut(s)	517
SinI	GGWCC	6 cut(s)	55, 76, 161, 196, 302, 790
SlaI	CTCGAG	2 cut(s)	95, 1814
SmaI	CCCGGG	1 cut(s)	128
SmiMI	CAYNNNNRTG	2 cut(s)	1638, 2523
SmlI	CTYRAG	5 cut(s)	95, 721, 1802, 1814, 2381
SmoI	CTYRAG	5 cut(s)	95, 721, 1802, 1814, 2381
SpeI	ACTAGT	1 cut(s)	2152
SseBI	AGGCCT	1 cut(s)	1492
SsiI	CCGC	3 cut(s)	241, 434, 2317
SspMI	CTAG	5 cut(s)	270, 485, 1062, 2114, 2153
SstI	GAGCTC	1 cut(s)	958
StuI	AGGCCT	1 cut(s)	1492
StyD4I	CCNGG	8 cut(s)	57, 79, 125, 126, 400, 1179, 1623, 1719
StyI	CCWWGG	3 cut(s)	1055, 1487, 1644
TaaI	ACNGT	7 cut(s)	118, 359, 474, 1680, 1977, 2033, 2470
TaiI	ACGT	3 cut(s)	484, 1171, 1276
TaqI	TCGA	8 cut(s)	96, 458, 517, 1150, 1650, 1815, 1864, 2485
TatI	WGTACW	2 cut(s)	2007, 2470
TauI	GCSGC	2 cut(s)	243, 437
TfiI	GAWTC	7 cut(s)	917, 1049, 1133, 1463, 1866, 1935, 1982
Tru1I	TTAA	6 cut(s)	248, 444, 1100, 1509, 1734, 2382
Tru9I	TTAA	6 cut(s)	248, 444, 1100, 1509, 1734, 2382
TscAI	CASTG	4 cut(s)	214, 1685, 1926, 2038
TseFI	GTSAC	7 cut(s)	118, 146, 353, 1520, 1539, 1757, 2027
Tsp45I	GTSAC	7 cut(s)	118, 146, 353, 1520, 1539, 1757, 2027
TspGWI	ACGGA	5 cut(s)	188, 714, 1269, 1572, 1586
TspMI	CCCGGG	1 cut(s)	126
TspRI	CASTG	4 cut(s)	214, 1685, 1926, 2038
Tth111I	GACNNNGTC	2 cut(s)	351, 794
Vha464I	CTTAAG	1 cut(s)	2381
VpaK11BI	GGWCC	6 cut(s)	55, 76, 161, 196, 302, 790
XagI	CCTNNNNNAGG	1 cut(s)	457
XapI	RAATTY	6 cut(s)	362, 494, 1821, 2182, 2206, 2269
XceI	RCATGY	1 cut(s)	639
XhoI	CTCGAG	2 cut(s)	95, 1814
XmaI	CCCGGG	1 cut(s)	126
XmiI	GTMKAC	2 cut(s)	1150, 1914
XmnI	GAANNNNTTC	1 cut(s)	2182
XspI	CTAG	5 cut(s)	270, 485, 1062, 2114, 2153
ZraI	GACGTC	1 cut(s)	1274
ZrmI	AGTACT	1 cut(s)	2472
Zsp2I	ATGCAT	1 cut(s)	1300

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.