Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0003250

Physical Location & Seq

Genomic Coordinates Reverse (-)

27650393 .. 27654593

Gene Structure 4201 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1132400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2733 bp

ATGCTGGAATTACAGGACAAGGAATTGGACCCTGGCTCTGACGAGCCTGAGGCCCCTTTGCCTCTCACCGTCACTTCCCGGGCCTTGTATATGTTGGGTGACATTGCCGCAGGGCAGGCATATAGAATCACGCAATGGATGGAACTGGTTCGTAAACGGAGTGCCAAATATCACTCCTCTGGCTTCCCCCACTACCTTCCGAGGTTCGACAGTATGCCTTCGAGTGTAGGAGACTTTGTTGATGATTGGGAAGGTTCTCTTCCCTGTGAGCAAACCACGGAAGTCAATCTCTGGGAAAGACTTGGTAAAGCTGCTGCATTGGACATTGAGTCGTGTTATTTTTCCTGGGATAGGCTCTCTTCGCTTCATCACACTGAGCATAGTAGTAGCAATGACAATTCTGAGGATGAAATGAATAAAGCTCTTGAGGTAACTGTAAATTCGGGGGGCGTTGTCTTCTTTGGCCTATTCAACCAGCCAGGGAATGATGATGCTTCTACTAAGGAAGCGGCGGCTGTTATAAAGATATCATCATCAAGAATGGCCACACAATCTGAACGCCTTGGATATGAATTTGCAAAGTGGCTTGGAGTTCGAACTCCTCAGGCCAGAGTCATTCATAATTGTAGCTTGGAGTGGCTGCAGATGAAGGAAGCTGCAGAGAAAGCAAGAGATGCAGCAAGTTCAGAAGGAGATGAATGTGGGGAAATGACGTGCTCAGAACTTTTGGAAGCTCTTGAACTTAGCCGATGCCTTTTGCTCATAAGCTATGTTCAGGGATCTCCTTTACTTGAAAGCCCTACTGTATTTGAGTCGAGGGAAACTGCAGAAAAAACAGCGGCTGCTCTTGGTAGGATCTTGATGTTGGATCTTGTCATCAGAAATGAGGATAGGCTTCCTTGCCATAAGCTCAGATGGCGTGGAAATTCCGCAAATCTATTGTTGGCTGACAAAATGGCTTTGGGAAATATGGATAGATTGGAGGAAGCCTTCGATTCTGCAATCAAGCGATACAAACCAAGAGTAATCAGAGGTCTTCATAAGGATAGAAGGGCAACTTCAGTAGATAGCAAATTGGGTACTCATAATGCCGTAACAGTAACACAGACCTCTGATCTTTCAGATATTATAGAGTCACCAAGATCTATACAGAGTGAACTATCAGATCATTCGGTGTTTTCTGATTTTCCAGTTGTGGCTATTGACTCTGGGGTTCCTCGTAGACCTCCTGCTGGAAAGCGTGCAAATGACCAGGAAATTTATCCCAGGTTGGTTGAGTTACTACTCAACAGTTCTGAGTACTCCTCTAATGTGTTGCATGAAATAACATTAGGGAAGTTAGGACGTTCTCCCATACAAGAAACTGATGCATCTGATATACCAGCGTATGAGATGGCTTCAGTTGTTAAAAAATTTCGTAGTGGATTCCGTGCTGCTCTTAGGGATCTGCAGGGATTCCATATATTCTTACTCACACTTCATCAAAGACTGGAGAACTTGTTACGAATATTCTTCAATATTATAGATAGAATATCCTCAGGGGAGTCCGACAAAGAGGATTTGGCAGTTCCTGAGTCTCCCTCTCATGCTGCTGGAAGTTTTCATTGTGCTTCTGCACTAAGTAAGGAACGACTTGTCAATGAGAACAATCCTGATTTTAGTGATTCCGAGTCACAGAGAACTGCTCTAAGGTCTTCGTCTTCTGGAAATAAAGAAGGCTATGACTGCAGCTCTCCCATGTCAAGGGATAGTTGGCATGGGAGGTTCTCTAAAGGAAGTGCGGAGCCCCTCAGTAGTCTGCGTTTGACAGCAAAGCTTCGGGATTTCCATAAATACGCCAAGGTTGATGCTGAATCAAACAAAGAACTGGAACAGTGGAATGAAATGCTAAAGAGTGATGTTATCAAACTATGTCAAGAGAACAATTTTAATTCTGGGTTTTTCGAGGGTAGTGACAACAATGGTGTTGTTGATGCTTATGAATTGAAGGTCAGACTTGAGCACATTCTTGAGAGGATTGCATTGATATCGGAGGCTGCAAATACAGAGAGGCCATCTCGAGTCACAAGTTCCATGTTCATCGGTGGAGCCTTGGCTGCACGATCTGTATTCACGCTGCAACGCTTAGGAATTACTCATGTATTGTGTTTGTGCTCCAATGAAATTGGACAAGCAGATTCTCAGTTTCCTGATCTATTCGAGTACAAGAATTTTGCTATATGCGACAATGACGATACAAATATCAGCACCATCTTTGATGAAGCTGTCAGTTTTACTGATCGTGTTGAACAAGAAAGAGGGAAGGTTCTCGTCCATTGCTTCGAGGGGAAAAGTAGAAGTGCCACCCTAGTGCTTGCATACCTAATGCTGAGAAAGAATTGCACTCTATTAGAGGCGTGGAATTCTCTAAAACAAGTTCATCGTCGGGCGCAACCCAATGATGGTTTTGCAAAGATCCTAGTGGATCTGGATAAGAAACTTCATGGGAGAGTTTCCATGGAGTGGCAACAGCGGAAGCCTACGATGAAAGTTTGCCCAATCTGCGGGGTAAATGCAGGTCTGAGTAGCAGCTCGCTTAAGCTTCATCTGCAGAAATCACACAAGAAGCTATCGTCAGGTAGCGTCGATAGTGCAATGACAATGGAGATACAAAAGGCTTTGACAGTGCTAAAAATTAGCCGAGGCGGAAGCGTCATCCCTAAACAGAGGTCTCATTCAGATGTGGAAAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000226 GO:0001101 GO:0003674 GO:0003824 GO:0004721 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007010 GO:0007017 GO:0008138 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009987 GO:0010033 GO:0010119 GO:0010468 GO:0016043 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019222 GO:0019538 GO:0030865 GO:0031122 GO:0033993 GO:0036211 GO:0042221 GO:0042578 GO:0043170 GO:0043412 GO:0043622 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0060255 GO:0065007 GO:0071704 GO:0071840 GO:0097305 GO:0097435 GO:0140096 GO:1901564 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Act-Frag_cataly DSPc

Protein Analysis

911

Amino Acids

101.43

Weight (kDa)

5.84

Isoelectric Point (pI)

49.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Act-Frag_cataly	PF09192	94 - 349	8.9e-44	Actin-fragmin kinase, catalytic
DSPc	PF00782	703 - 824	2.9e-24	Dual specificity phosphatase, catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0003250)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G23720 AT5G23720 AT5G23720
fragaria_vesca	FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220
malus_domestica	MD06G1132400.v1.1 MD14G1148800.v1.1
prunus_persica	Prupe.5G143400_v2.0.a1
pyrus_communis	pycom06g12400 pycom14g12360
rosa_chinensis	RchiOBHm_Chr4g0416471 RchiOBHm_Chr7g0188941 RchiOBHm_Chr7g0194331
rosa_laevigata	RLG00000004049 RLG00000004648
rosa_multiflora	Rmu_co8071116.1_g000001 Rmu_sc0007831.1_g000001 Rmu_sc0032883.1_g000001 Rmu_ssc0000113.1_g000006
rosa_roxburghii	Rroxscaffold_3G00265920
rosa_rugosa	Rorug06G0494800 Rorug06G0494900 Rorug06G0495000
rosa_samantha	Rh7AG099700 Rh7BG101800 Rh7CG103100 Rh7CG146600 Rh7DG101600 Rh7DG144200
rosa_wichuraiana	Rw7G008590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	521
Acc36I	ACCTGC	1 cut(s)	2546
AccB7I	CCANNNNNTGG	1 cut(s)	2502
AccI	GTMKAC	1 cut(s)	1222
AciI	CCGC	9 cut(s)	108, 509, 512, 839, 930, 1781, 2512, 2544, 2685
AclWI	GGATC	6 cut(s)	787, 863, 876, 1452, 2449, 2472
AcoI	YGGCCR	1 cut(s)	543
AcsI	RAATTY	7 cut(s)	439, 572, 925, 1257, 1412, 2209, 2401
AcuI	CTGAAG	2 cut(s)	1044, 1383
AfaI	GTAC	3 cut(s)	1081, 1301, 2204
AfiI	CCNNNNNNNGG	6 cut(s)	351, 1232, 1743, 2441, 2502, 2543
AflII	CTTAAG	1 cut(s)	2576
AgsI	TTSAA	6 cut(s)	472, 740, 794, 1516, 1987, 2288
AhdI	GACNNNNNGTC	1 cut(s)	328
AjiI	CACGTC	1 cut(s)	714
AjnI	CCWGG	5 cut(s)	31, 344, 478, 1251, 1265
AleI	CACNNNNGTG	1 cut(s)	2348
Alw21I	GWGCWC	3 cut(s)	719, 2004, 2156
Alw26I	GTCTC	3 cut(s)	225, 1581, 2715
AlwI	GGATC	6 cut(s)	787, 863, 876, 1452, 2449, 2472
AlwNI	CAGNNNCTG	2 cut(s)	842, 1571
Ama87I	CYCGRG	2 cut(s)	78, 2058
AoxI	GGCC	6 cut(s)	51, 81, 463, 543, 606, 2051
ApoI	RAATTY	7 cut(s)	439, 572, 925, 1257, 1412, 2209, 2401
Asp700I	GAANNNNTTC	1 cut(s)	147
AspLEI	GCGC	1 cut(s)	2431
AspS9I	GGNCC	3 cut(s)	28, 52, 81
AsuC2I	CCSGG	2 cut(s)	79, 80
AsuHPI	GGTGA	3 cut(s)	58, 110, 1128
AsuII	TTCGAA	1 cut(s)	595
AvaI	CYCGRG	2 cut(s)	78, 2058
AvaII	GGWCC	1 cut(s)	28
AxyI	CCTNAGG	3 cut(s)	48, 603, 1537
BalI	TGGCCA	1 cut(s)	545
BanII	GRGCYC	1 cut(s)	1788
BbsI	GAAGAC	4 cut(s)	448, 1028, 1686, 1692
Bbv12I	GWGCWC	3 cut(s)	719, 2004, 2156
BccI	CCATC	6 cut(s)	133, 909, 1387, 2062, 2258, 2435
BceAI	ACGGC	1 cut(s)	1076
BcgI	CGANNNNNNTGC	2 cut(s)	2010, 2044
BciT130I	CCWGG	5 cut(s)	33, 346, 480, 1253, 1267
BcnI	CCSGG	2 cut(s)	79, 80
BcoDI	GTCTC	3 cut(s)	225, 1581, 2715
BfaI	CTAG	2 cut(s)	2348, 2459
BfmI	CTRYAG	6 cut(s)	641, 657, 825, 1448, 1726, 2588
BfrI	CTTAAG	1 cut(s)	2576
BfuAI	ACCTGC	1 cut(s)	2546
BglII	AGATCT	1 cut(s)	1142
BmcAI	AGTACT	1 cut(s)	1301
Bme1390I	CCNGG	7 cut(s)	33, 79, 80, 346, 480, 1253, 1267
Bme18I	GGWCC	1 cut(s)	28
BmeRI	GACNNNNNGTC	1 cut(s)	328
BmeT110I	CYCGRG	2 cut(s)	78, 2058
BmgBI	CACGTC	1 cut(s)	714
BmgT120I	GGNCC	3 cut(s)	28, 52, 81
BmiI	GGNNCC	5 cut(s)	30, 54, 1215, 1785, 2089
BmrFI	CCNGG	7 cut(s)	33, 79, 80, 346, 480, 1253, 1267
BmsI	GCATC	7 cut(s)	481, 664, 740, 1357, 1379, 1837, 1963
BpiI	GAAGAC	4 cut(s)	448, 1028, 1686, 1692
BplI	GAGNNNNNCTC	8 cut(s)	1289, 1321, 1565, 1597, 1669, 1701, 2041, 2073
BpmI	CTGGAG	1 cut(s)	1511
Bpu10I	CCTNAGC	1 cut(s)	2125
Bpu14I	TTCGAA	1 cut(s)	595
BpuEI	CTTGAG	3 cut(s)	446, 2018, 2030
BpuMI	CCSGG	2 cut(s)	79, 80
BsaI	GGTCTC	1 cut(s)	2715
BsaXI	ACNNNNNCTCC	4 cut(s)	222, 252, 1484, 1514
Bsc4I	CCNNNNNNNGG	6 cut(s)	351, 1232, 1743, 2441, 2502, 2543
Bse1I	ACTGG	4 cut(s)	150, 1190, 1494, 1872
Bse21I	CCTNAGG	3 cut(s)	48, 603, 1537
Bse3DI	GCAATG	5 cut(s)	102, 140, 397, 2314, 2640
BseBI	CCWGG	5 cut(s)	33, 346, 480, 1253, 1267
BseGI	GGATG	3 cut(s)	144, 412, 2694
BseLI	CCNNNNNNNGG	6 cut(s)	351, 1232, 1743, 2441, 2502, 2543
BseMI	GCAATG	5 cut(s)	102, 140, 397, 2314, 2640
BseNI	ACTGG	4 cut(s)	150, 1190, 1494, 1872
BseRI	GAGGAG	3 cut(s)	166, 591, 1294
BsgI	GTGCAG	2 cut(s)	1599, 2082
BshFI	GGCC	6 cut(s)	53, 83, 465, 545, 608, 2053
BsiHKAI	GWGCWC	3 cut(s)	719, 2004, 2156
BsiHKCI	CYCGRG	2 cut(s)	78, 2058
BsiSI	CCGG	1 cut(s)	79
BslI	CCNNNNNNNGG	6 cut(s)	351, 1232, 1743, 2441, 2502, 2543
BsmAI	GTCTC	3 cut(s)	225, 1581, 2715
BsnI	GGCC	6 cut(s)	53, 83, 465, 545, 608, 2053
Bso31I	GGTCTC	1 cut(s)	2715
BsoBI	CYCGRG	2 cut(s)	78, 2058
Bsp119I	TTCGAA	1 cut(s)	595
Bsp1286I	GDGCHC	4 cut(s)	719, 1788, 2004, 2156
Bsp19I	CCATGG	1 cut(s)	2496
BspACI	CCGC	9 cut(s)	108, 509, 512, 839, 930, 1781, 2512, 2544, 2685
BspANI	GGCC	6 cut(s)	53, 83, 465, 545, 608, 2053
BspLI	GGNNCC	5 cut(s)	30, 54, 1215, 1785, 2089
BspMAI	CTGCAG	6 cut(s)	645, 661, 829, 1452, 1730, 2592
BspMI	ACCTGC	1 cut(s)	2546
BspPI	GGATC	6 cut(s)	787, 863, 876, 1452, 2449, 2472
BspT104I	TTCGAA	1 cut(s)	595
BspTI	CTTAAG	1 cut(s)	2576
BspTNI	GGTCTC	1 cut(s)	2715
BsrDI	GCAATG	5 cut(s)	102, 140, 397, 2314, 2640
BsrI	ACTGG	4 cut(s)	150, 1190, 1494, 1872
BssT1I	CCWWGG	4 cut(s)	562, 1839, 2091, 2496
Bst2UI	CCWGG	5 cut(s)	33, 346, 480, 1253, 1267
Bst4CI	ACNGT	8 cut(s)	70, 212, 436, 805, 1099, 1292, 1875, 2665
Bst6I	CTCTTC	2 cut(s)	264, 364
BstAFI	CTTAAG	1 cut(s)	2576
BstAPI	GCANNNNNTGC	1 cut(s)	674
BstBI	TTCGAA	1 cut(s)	595
BstC8I	GCNNGC	4 cut(s)	117, 1242, 2355, 2573
BstDSI	CCRYGG	2 cut(s)	276, 2496
BstENI	CCTNNNNNAGG	1 cut(s)	349
BstF5I	GGATG	3 cut(s)	144, 412, 2694
BstHHI	GCGC	1 cut(s)	2431
BstMAI	GTCTC	3 cut(s)	225, 1581, 2715
BstMWI	GCNNNNNNNGC	9 cut(s)	116, 361, 665, 674, 916, 1725, 2096, 2541, 2587
BstNI	CCWGG	5 cut(s)	33, 346, 480, 1253, 1267
BstSCI	CCNGG	7 cut(s)	31, 77, 78, 344, 478, 1251, 1265
BstSFI	CTRYAG	6 cut(s)	641, 657, 825, 1448, 1726, 2588
BstV2I	GAAGAC	4 cut(s)	448, 1028, 1686, 1692
BstX2I	RGATCY	7 cut(s)	779, 855, 868, 1142, 1444, 2454, 2464
BstYI	RGATCY	7 cut(s)	779, 855, 868, 1142, 1444, 2454, 2464
Bsu36I	CCTNAGG	3 cut(s)	48, 603, 1537
BsuRI	GGCC	6 cut(s)	53, 83, 465, 545, 608, 2053
BtgI	CCRYGG	2 cut(s)	276, 2496
BtrI	CACGTC	1 cut(s)	714
BtsCI	GGATG	3 cut(s)	144, 412, 2694
BtsIMutI	CAGTG	3 cut(s)	372, 1880, 2670
BveI	ACCTGC	1 cut(s)	2546
Cac8I	GCNNGC	4 cut(s)	117, 1242, 2355, 2573
CaiI	CAGNNNCTG	2 cut(s)	842, 1571
CfoI	GCGC	1 cut(s)	2431
Cfr13I	GGNCC	3 cut(s)	28, 52, 81
Cfr9I	CCCGGG	1 cut(s)	78
CseI	GACGC	2 cut(s)	2611, 2680
Csp6I	GTAC	3 cut(s)	1080, 1300, 2203
CviAII	CATG	8 cut(s)	1319, 1586, 1738, 1757, 2074, 2138, 2483, 2497
CviQI	GTAC	3 cut(s)	1080, 1300, 2203
DriI	GACNNNNNGTC	1 cut(s)	328
EaeI	YGGCCR	1 cut(s)	543
Eam1104I	CTCTTC	2 cut(s)	264, 364
Eam1105I	GACNNNNNGTC	1 cut(s)	328
EarI	CTCTTC	2 cut(s)	264, 364
EciI	GGCGGA	1 cut(s)	2700
Eco130I	CCWWGG	4 cut(s)	562, 1839, 2091, 2496
Eco24I	GRGCYC	1 cut(s)	1788
Eco31I	GGTCTC	1 cut(s)	2715
Eco32I	GATATC	2 cut(s)	528, 2028
Eco47I	GGWCC	1 cut(s)	28
Eco57I	CTGAAG	2 cut(s)	1044, 1383
Eco81I	CCTNAGG	3 cut(s)	48, 603, 1537
Eco88I	CYCGRG	2 cut(s)	78, 2058
EcoNI	CCTNNNNNAGG	1 cut(s)	349
EcoO109I	RGGNCCY	1 cut(s)	52
EcoRI	GAATTC	1 cut(s)	2401
EcoRII	CCWGG	5 cut(s)	31, 344, 478, 1251, 1265
EcoRV	GATATC	2 cut(s)	528, 2028
EcoT14I	CCWWGG	4 cut(s)	562, 1839, 2091, 2496
EcoT22I	ATGCAT	1 cut(s)	1372
EcoT38I	GRGCYC	1 cut(s)	1788
ErhI	CCWWGG	4 cut(s)	562, 1839, 2091, 2496
FaeI	CATG	8 cut(s)	1322, 1589, 1741, 1760, 2077, 2141, 2486, 2500
FalI	AAGNNNNNCTT	4 cut(s)	243, 275, 1042, 1074
FatI	CATG	8 cut(s)	1318, 1585, 1737, 1756, 2073, 2137, 2482, 2496
FauI	CCCGC	1 cut(s)	2537
FblI	GTMKAC	1 cut(s)	1222
FokI	GGATG	3 cut(s)	151, 419, 2681
FriOI	GRGCYC	1 cut(s)	1788
FspBI	CTAG	2 cut(s)	2348, 2459
GlaI	GCGC	1 cut(s)	2430
GsuI	CTGGAG	1 cut(s)	1511
HaeIII	GGCC	6 cut(s)	53, 83, 465, 545, 608, 2053
HapII	CCGG	1 cut(s)	79
HgaI	GACGC	2 cut(s)	2611, 2680
HhaI	GCGC	1 cut(s)	2431
Hin1II	CATG	8 cut(s)	1322, 1589, 1741, 1760, 2077, 2141, 2486, 2500
Hin6I	GCGC	1 cut(s)	2429
HinP1I	GCGC	1 cut(s)	2429
HindIII	AAGCTT	2 cut(s)	1814, 2579
HpaII	CCGG	1 cut(s)	79
HphI	GGTGA	3 cut(s)	58, 110, 1128
Hpy166II	GTNNAC	3 cut(s)	155, 1157, 1223
Hpy8I	GTNNAC	3 cut(s)	155, 1157, 1223
Hpy99I	CGWCG	2 cut(s)	2427, 2627
HpyCH4III	ACNGT	8 cut(s)	70, 212, 436, 805, 1099, 1292, 1875, 2665
HpyCH4IV	ACGT	2 cut(s)	713, 1345
HpyF10VI	GCNNNNNNNGC	9 cut(s)	116, 361, 665, 674, 916, 1725, 2096, 2541, 2587
HpySE526I	ACGT	2 cut(s)	713, 1345
Hsp92II	CATG	8 cut(s)	1322, 1589, 1741, 1760, 2077, 2141, 2486, 2500
HspAI	GCGC	1 cut(s)	2429
LmnI	GCTCC	3 cut(s)	1783, 2087, 2159
LweI	GCATC	7 cut(s)	481, 664, 740, 1357, 1379, 1837, 1963
MaeI	CTAG	2 cut(s)	2348, 2459
MaeII	ACGT	2 cut(s)	713, 1345
MboII	GAAGA	7 cut(s)	251, 351, 448, 1028, 1504, 1686, 1692
MflI	RGATCY	7 cut(s)	779, 855, 868, 1142, 1444, 2454, 2464
MhlI	GDGCHC	4 cut(s)	719, 1788, 2004, 2156
MlsI	TGGCCA	1 cut(s)	545
MluNI	TGGCCA	1 cut(s)	545
MlyI	GAGTC	9 cut(s)	338, 621, 821, 1142, 1199, 1553, 1583, 1679, 2070
MmeI	TCCRAC	2 cut(s)	846, 1572
Mox20I	TGGCCA	1 cut(s)	545
Mph1103I	ATGCAT	1 cut(s)	1372
MroXI	GAANNNNTTC	1 cut(s)	147
MscI	TGGCCA	1 cut(s)	545
MseI	TTAA	4 cut(s)	1407, 1929, 2577, 2731
MslI	CAYNNNNRTG	2 cut(s)	2348, 2718
Msp20I	TGGCCA	1 cut(s)	545
MspA1I	CMGCKG	2 cut(s)	839, 2512
MspCI	CTTAAG	1 cut(s)	2576
MspI	CCGG	1 cut(s)	79
MspR9I	CCNGG	7 cut(s)	33, 79, 80, 346, 480, 1253, 1267
MvaI	CCWGG	5 cut(s)	33, 346, 480, 1253, 1267
MwoI	GCNNNNNNNGC	9 cut(s)	116, 361, 665, 674, 916, 1725, 2096, 2541, 2587
NciI	CCSGG	2 cut(s)	79, 80
NcoI	CCATGG	1 cut(s)	2496
NlaIII	CATG	8 cut(s)	1322, 1589, 1741, 1760, 2077, 2141, 2486, 2500
NlaIV	GGNNCC	5 cut(s)	30, 54, 1215, 1785, 2089
NmeAIII	GCCGAG	1 cut(s)	2705
NmuCI	GTSAC	6 cut(s)	70, 98, 1134, 1671, 1952, 2062
NsiI	ATGCAT	1 cut(s)	1372
NspV	TTCGAA	1 cut(s)	595
OliI	CACNNNNGTG	1 cut(s)	2348
PaeR7I	CTCGAG	1 cut(s)	2058
PdmI	GAANNNNTTC	1 cut(s)	147
PfeI	GAWTC	7 cut(s)	126, 995, 1425, 1455, 1664, 1853, 2177
PflMI	CCANNNNNTGG	1 cut(s)	2502
PleI	GAGTC	9 cut(s)	337, 620, 820, 1141, 1199, 1552, 1582, 1678, 2069
PpsI	GAGTC	9 cut(s)	337, 620, 820, 1141, 1199, 1552, 1582, 1678, 2069
PsiI	TTATAA	1 cut(s)	521
Psp6I	CCWGG	5 cut(s)	31, 344, 478, 1251, 1265
PspGI	CCWGG	5 cut(s)	31, 344, 478, 1251, 1265
PspN4I	GGNNCC	5 cut(s)	30, 54, 1215, 1785, 2089
PspPI	GGNCC	3 cut(s)	28, 52, 81
PstI	CTGCAG	6 cut(s)	645, 661, 829, 1452, 1730, 2592
PstNI	CAGNNNCTG	2 cut(s)	842, 1571
PsuI	RGATCY	7 cut(s)	779, 855, 868, 1142, 1444, 2454, 2464
RsaI	GTAC	3 cut(s)	1081, 1301, 2204
RsaNI	GTAC	3 cut(s)	1080, 1300, 2203
RseI	CAYNNNNRTG	2 cut(s)	2348, 2718
SaqAI	TTAA	4 cut(s)	1407, 1929, 2577, 2731
Sau96I	GGNCC	3 cut(s)	28, 52, 81
ScaI	AGTACT	1 cut(s)	1301
SchI	GAGTC	9 cut(s)	338, 621, 821, 1142, 1199, 1553, 1583, 1679, 2070
ScrFI	CCNGG	7 cut(s)	33, 79, 80, 346, 480, 1253, 1267
SduI	GDGCHC	4 cut(s)	719, 1788, 2004, 2156
SfaNI	GCATC	7 cut(s)	481, 664, 740, 1357, 1379, 1837, 1963
SfcI	CTRYAG	6 cut(s)	641, 657, 825, 1448, 1726, 2588
Sfr274I	CTCGAG	1 cut(s)	2058
SfuI	TTCGAA	1 cut(s)	595
SinI	GGWCC	1 cut(s)	28
SlaI	CTCGAG	1 cut(s)	2058
SmaI	CCCGGG	1 cut(s)	80
SmiMI	CAYNNNNRTG	2 cut(s)	2348, 2718
SmlI	CTYRAG	5 cut(s)	425, 1997, 2009, 2058, 2576
SmoI	CTYRAG	5 cut(s)	425, 1997, 2009, 2058, 2576
SsiI	CCGC	9 cut(s)	108, 509, 512, 839, 930, 1781, 2512, 2544, 2685
SspI	AATATT	2 cut(s)	1509, 1519
SspMI	CTAG	2 cut(s)	2348, 2459
StyD4I	CCNGG	7 cut(s)	31, 77, 78, 344, 478, 1251, 1265
StyI	CCWWGG	4 cut(s)	562, 1839, 2091, 2496
TaaI	ACNGT	8 cut(s)	70, 212, 436, 805, 1099, 1292, 1875, 2665
TaiI	ACGT	2 cut(s)	716, 1348
TatI	WGTACW	2 cut(s)	1299, 2202
TauI	GCSGC	4 cut(s)	110, 512, 515, 842
TfiI	GAWTC	7 cut(s)	126, 995, 1425, 1455, 1664, 1853, 2177
Tru1I	TTAA	4 cut(s)	1407, 1929, 2577, 2731
Tru9I	TTAA	4 cut(s)	1407, 1929, 2577, 2731
TscAI	CASTG	3 cut(s)	379, 1880, 2670
TseFI	GTSAC	6 cut(s)	70, 98, 1134, 1671, 1952, 2062
Tsp45I	GTSAC	6 cut(s)	70, 98, 1134, 1671, 1952, 2062
TspGWI	ACGGA	3 cut(s)	172, 293, 1418
TspMI	CCCGGG	1 cut(s)	78
TspRI	CASTG	3 cut(s)	379, 1880, 2670
Van91I	CCANNNNNTGG	1 cut(s)	2502
Vha464I	CTTAAG	1 cut(s)	2576
VpaK11BI	GGWCC	1 cut(s)	28
XagI	CCTNNNNNAGG	1 cut(s)	349
XapI	RAATTY	7 cut(s)	439, 572, 925, 1257, 1412, 2209, 2401
XhoI	CTCGAG	1 cut(s)	2058
XmaI	CCCGGG	1 cut(s)	78
XmiI	GTMKAC	1 cut(s)	1222
XmnI	GAANNNNTTC	1 cut(s)	147
XspI	CTAG	2 cut(s)	2348, 2459
ZrmI	AGTACT	1 cut(s)	1301
Zsp2I	ATGCAT	1 cut(s)	1372

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.