Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0003250

Physical Location & Seq

Genomic Coordinates Reverse (-)

24154886 .. 24160067

Gene Structure 5182 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1148800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2784 bp

ATGGCAAAAGAGCAAAGCCAGGAGCCTGCAATCACCAACCTTCTGCAGCTCCAGGACAAGGAGGAATACAAGGAGTTGGACCCTGGATCTGAGGAGCCTGAGGCCCCTTTGCCTCTCACCGTCACTTCCCGGGTCTTGTATATGTTGGGTGACATTGCCGCAGGGCCGGCATATAGAATCACGCAATGGCTGGAACTGGTTCGTAAACGGAGTGCTAAGTATCACTCGTCTGGCTTCCCCCACCGCCTTCCAAGGTTTGACAGTATGCCTTCGAGTGTAGGAGACTTTGTTGATGATTGGAAAGGTTCTCTTCCCTGTGAGCAAACCACGGAAGTCAATCTCTGGGAAAGACTTGGCAAAGCTGCTACATTGGATATTGAGTCGAGTTATTTTTCCTGGGATAGGCTCTCGTCGCTTCATCACACTGAGCATAGTAGTAGCAATGACAATTCTGAGGATGAAATGAATAAAGCTCTTGAGGTAACTGTAAATTCGGGGGGTGTTGTCTTCTTTGGCCTGTTCAACCAGCCAGGGAATGATGATGCTTCTACTAAGGAAGCGGCGGCTGTTATAAAGATTTCATCATCAAGGATGGCCACGCAATCTGAACGCCTTGGATATGAAATTGCAAAGTGGCTTGGAGTTCGAACTCCTCAGGCCAGAGTCATTCATAATTGTAGCTTGGAATGGCTCCAGATTAAGGAAGCTGCAGAGAAAGCAAGAGATGCAGCAAGTGCAGAAGGAGATGAATGTGGGGAAATGACGTGTTCAGAACTTTTGGAAGCTCTTGAACTTAGCCGATGCCTTTTGCTCATGAGCTATGTTCACGGATCTCCTTTACTTGAAAGCCCAACTGTATTTGAGTCGAGGGAAACTGCAGAAAAAACAGCGGCTGCTCTTGGTAGGATCTTGATGTTGGATCTTGTCATCAGAAATGAGGATAGGCTTCCTTGCCATAAGCTCAGATGGCGTGGAAATTCTGCAAATCTATTGTTGGCTGACAAAATGTCTTTGGGAAATATGGATAGTTTGGGGGAATCCCTTGATTCTACAATCAAGCGATACAAACCAAGAGTAATCAGAGGTCTTCATAAGGATAGAAGGGTAACTTCAGTAGATAGCAAATTAGGTACTCATAATGCCGTATTGGTACCACAGACTTCTGATCTTTCAGATATTGTAGAGTCACCAAGATCTATTAAGAGTGCACTATCAGATGATGCAGTGCATTCTGATTTTCCAGTTTTGGCTATTGACTCTGGGGTTCCTCGTCGACCTCCTGCTGGAAAGCGCGCAAATGACCAGGAAATTTATCCCAGGTTGGTTGAGTTACTACTCAACAGTTCTGATTACTCATCTAATGTGTTGCATGAAATAACATTAGGGAAGTTAGGTCGTTCTACCATACAAGAAACTGATGCATCTGATATGCCGGCGTTTGAGATGGCTTCGGTTGTTCAAGAATTTCGTGGTGGATTCCGAGCTGCTCTTCGGGATCTGCAGGGATTCCATATATTCTTACTCACACTTCATCAAAGACTGGAGAACTTGTTACGAATATTCTTCAATATTATAGATAGAATATCCTCAGGGGAGTCTGACAAAGAGGATTTGGCAGTTCCTGAGTCTTCCTCTCACGCTGCTGGAAGTGTTCATTGTGCTTCTCCACCAAGTAAGGAAAGACTTATCAATGAGAACAATCCTGATTTTAGTGATTCTGAATTACAGAGAACTGCTCCAAGGTCTTCATATTCCGGAAATAAAGAAGGCTATGACTGCAGCTCGCCCATGTCAAGGGATGGTTTGCATGGGAGATTCTCTAAAGGAATTGCGGAGCCCCTCCGTAGTTTGCGTTTGACAGCAAAGCTTCGGGATTTCCATAAATACGCCAAGGTTGATGCTGAATCAAACAAAGAACTGGAACAGTGGAATGAAATGCTAAAGTTTGATGCTGTCAAACTATGTCAAGAGAACAATTTTAATTCTGGGTTTTTCGAGGGTAGTGACAACAATGGTGTTGTCGATGCTTATGAATTGAAGGTTAGACTTGAGCACATTCTTGAGAGGATTGCATTGATATCTGGTGCTGCAAATACAGAGAGGCCATCTCGAGTTACAAGTTGCATGTTCATCGGTGGGGCCCTGGCTGCACGATCTGTATTCACGCTGCAACGCTTGGAAATTACTCATGTGTTGTGTTTGTGCTCCAATGAAATTGGACAAGCTGATTCTCAGTTTCCTGATCTATTTGAGTACAAGAATTTTGCTATATGCGACAATGACGATACAAACATCAGCACCATCTTTGATGAAGCTATTGGTTTTATTGATCGTGTTGAACAAAAAAGAGGGAAGGTTCTCGTCCATTGCTTCGAGGGGAAAAGTAGAAGTGCCACCCTTGTGCTTGCCTACCTAATGCTGAGAAAGAATTGCACTCTATTAGAGGCGTGGACGTCTCTAAAACAAGTTCATCGTCGGGCGCAACCAAATGATGGTTTTGCAAAGATCCTAGTGGATCTGGATAAGAAACTTCACGGGAGAGTTTCCATGGAGTGGCAACAGCGGAAGCCTACAATGAAAGTTTGCCCAATCTGCGGGGTGAATGCAGGTCTGAGTAGCAGCTCGCTTAAGCTTCATTTGCAGAAATCACACAAGAAGCTATCGTCAGGTAGTGTCGATAGTGCAATGACAATGGAGATACAAAAGGCTTTGACAGCGCTAAAAATTAGCCGAGGCGGAAGCGTCAGCCCTAAACAGAGGTCTCATTCAGATGTGGAAGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000226 GO:0001101 GO:0003674 GO:0003824 GO:0004721 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007010 GO:0007017 GO:0008138 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009987 GO:0010033 GO:0010119 GO:0010468 GO:0016043 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019222 GO:0019538 GO:0030865 GO:0031122 GO:0033993 GO:0036211 GO:0042221 GO:0042578 GO:0043170 GO:0043412 GO:0043622 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0060255 GO:0065007 GO:0071704 GO:0071840 GO:0097305 GO:0097435 GO:0140096 GO:1901564 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Act-Frag_cataly DSPc

Protein Analysis

928

Amino Acids

103.14

Weight (kDa)

5.96

Isoelectric Point (pI)

51.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Act-Frag_cataly	PF09192	111 - 336	3.9e-44	Actin-fragmin kinase, catalytic
DSPc	PF00782	720 - 841	3.8e-25	Dual specificity phosphatase, catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0003250)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G23720 AT5G23720 AT5G23720
fragaria_vesca	FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220
malus_domestica	MD06G1132400.v1.1 MD14G1148800.v1.1
prunus_persica	Prupe.5G143400_v2.0.a1
pyrus_communis	pycom06g12400 pycom14g12360
rosa_chinensis	RchiOBHm_Chr4g0416471 RchiOBHm_Chr7g0188941 RchiOBHm_Chr7g0194331
rosa_laevigata	RLG00000004049 RLG00000004648
rosa_multiflora	Rmu_co8071116.1_g000001 Rmu_sc0007831.1_g000001 Rmu_sc0032883.1_g000001 Rmu_ssc0000113.1_g000006
rosa_roxburghii	Rroxscaffold_3G00265920
rosa_rugosa	Rorug06G0494800 Rorug06G0494900 Rorug06G0495000
rosa_samantha	Rh7AG099700 Rh7BG101800 Rh7CG103100 Rh7CG146600 Rh7DG101600 Rh7DG144200
rosa_wichuraiana	Rw7G008590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	572
AatII	GACGTC	1 cut(s)	2456
Acc36I	ACCTGC	1 cut(s)	2597
Acc65I	GGTACC	1 cut(s)	1150
AccB1I	GGYRCC	1 cut(s)	1150
AccB7I	CCANNNNNTGG	2 cut(s)	2492, 2553
AccI	GTMKAC	1 cut(s)	1273
AccII	CGCG	1 cut(s)	1293
AccIII	TCCGGA	1 cut(s)	1754
AciI	CCGC	9 cut(s)	159, 244, 560, 563, 890, 1832, 2563, 2595, 2736
AclWI	GGATC	7 cut(s)	94, 838, 914, 927, 1503, 2500, 2523
AcoI	YGGCCR	1 cut(s)	594
AcsI	RAATTY	5 cut(s)	490, 976, 1308, 1463, 2260
AcuI	CTGAAG	1 cut(s)	1095
AcyI	GRCGYC	1 cut(s)	2453
AfaI	GTAC	3 cut(s)	1132, 1152, 2255
AfeI	AGCGCT	1 cut(s)	2718
AfiI	CCNNNNNNNGG	8 cut(s)	58, 402, 700, 1283, 1794, 2492, 2553, 2594
AflII	CTTAAG	1 cut(s)	2627
AflIII	ACRYGT	1 cut(s)	764
AgsI	TTSAA	7 cut(s)	523, 791, 845, 1460, 1567, 2038, 2339
AhdI	GACNNNNNGTC	1 cut(s)	1006
AjiI	CACGTC	1 cut(s)	765
AjnI	CCWGG	8 cut(s)	18, 51, 82, 395, 529, 1302, 1316, 2142
AleI	CACNNNNGTG	1 cut(s)	2399
Alw21I	GWGCWC	3 cut(s)	1210, 2055, 2207
Alw26I	GTCTC	3 cut(s)	276, 2460, 2766
Alw44I	GTGCAC	1 cut(s)	1206
AlwI	GGATC	7 cut(s)	94, 838, 914, 927, 1503, 2500, 2523
AlwNI	CAGNNNCTG	2 cut(s)	893, 1622
Ama87I	CYCGRG	2 cut(s)	129, 2109
Aor13HI	TCCGGA	1 cut(s)	1754
Aor51HI	AGCGCT	1 cut(s)	2718
AoxI	GGCC	7 cut(s)	102, 164, 514, 594, 657, 2102, 2139
ApaI	GGGCCC	1 cut(s)	2143
ApaLI	GTGCAC	1 cut(s)	1206
ApoI	RAATTY	5 cut(s)	490, 976, 1308, 1463, 2260
Asp700I	GAANNNNTTC	1 cut(s)	198
Asp718I	GGTACC	1 cut(s)	1150
AspLEI	GCGC	4 cut(s)	1293, 1295, 2482, 2719
AspS9I	GGNCC	5 cut(s)	79, 103, 164, 2139, 2140
AsuC2I	CCSGG	2 cut(s)	130, 131
AsuHPI	GGTGA	5 cut(s)	25, 109, 161, 1179, 2611
AsuII	TTCGAA	1 cut(s)	646
AvaI	CYCGRG	2 cut(s)	129, 2109
AvaII	GGWCC	1 cut(s)	79
AxyI	CCTNAGG	3 cut(s)	99, 654, 1588
BaeGI	GKGCMC	2 cut(s)	1210, 2143
BalI	TGGCCA	1 cut(s)	596
BanI	GGYRCC	1 cut(s)	1150
BanII	GRGCYC	2 cut(s)	1839, 2143
BbsI	GAAGAC	4 cut(s)	499, 1079, 1620, 1737
Bbv12I	GWGCWC	3 cut(s)	1210, 2055, 2207
BccI	CCATC	7 cut(s)	586, 960, 1438, 1793, 2113, 2309, 2486
BceAI	ACGGC	1 cut(s)	1127
BcgI	CGANNNNNNTGC	2 cut(s)	2113, 2147
BciT130I	CCWGG	8 cut(s)	20, 53, 84, 397, 531, 1304, 1318, 2144
BcnI	CCSGG	2 cut(s)	130, 131
BcoDI	GTCTC	3 cut(s)	276, 2460, 2766
BfaI	CTAG	1 cut(s)	2510
BfmI	CTRYAG	5 cut(s)	44, 708, 876, 1499, 1777
BfoI	RGCGCY	1 cut(s)	2720
BfrI	CTTAAG	1 cut(s)	2627
BfuAI	ACCTGC	1 cut(s)	2597
BglII	AGATCT	1 cut(s)	1193
Bme18I	GGWCC	1 cut(s)	79
BmeRI	GACNNNNNGTC	1 cut(s)	1006
BmeT110I	CYCGRG	2 cut(s)	129, 2109
BmgBI	CACGTC	1 cut(s)	765
BmgT120I	GGNCC	5 cut(s)	79, 103, 164, 2139, 2140
BmsI	GCATC	9 cut(s)	532, 715, 791, 1210, 1408, 1430, 1888, 1939, 2014
BpiI	GAAGAC	4 cut(s)	499, 1079, 1620, 1737
BplI	GAGNNNNNCTC	6 cut(s)	1616, 1648, 1720, 1752, 2092, 2124
BpmI	CTGGAG	3 cut(s)	35, 677, 1562
Bpu14I	TTCGAA	1 cut(s)	646
BpuEI	CTTGAG	3 cut(s)	497, 2069, 2081
BpuMI	CCSGG	2 cut(s)	130, 131
BsaHI	GRCGYC	1 cut(s)	2453
BsaI	GGTCTC	1 cut(s)	2766
BsaWI	WCCGGW	1 cut(s)	1754
BsaXI	ACNNNNNCTCC	4 cut(s)	273, 303, 1535, 1565
Bsc4I	CCNNNNNNNGG	8 cut(s)	58, 402, 700, 1283, 1794, 2492, 2553, 2594
Bse118I	RCCGGY	2 cut(s)	166, 1432
Bse1I	ACTGG	4 cut(s)	201, 1241, 1545, 1923
Bse21I	CCTNAGG	3 cut(s)	99, 654, 1588
Bse3DI	GCAATG	5 cut(s)	153, 191, 448, 2365, 2691
BseAI	TCCGGA	1 cut(s)	1754
BseBI	CCWGG	8 cut(s)	20, 53, 84, 397, 531, 1304, 1318, 2144
BseGI	GGATG	3 cut(s)	463, 597, 1804
BseLI	CCNNNNNNNGG	8 cut(s)	58, 402, 700, 1283, 1794, 2492, 2553, 2594
BseMI	GCAATG	5 cut(s)	153, 191, 448, 2365, 2691
BseNI	ACTGG	4 cut(s)	201, 1241, 1545, 1923
BsePI	GCGCGC	1 cut(s)	1291
BseRI	GAGGAG	2 cut(s)	107, 642
BseSI	GKGCMC	2 cut(s)	1210, 2143
BsgI	GTGCAG	2 cut(s)	756, 2133
Bsh1236I	CGCG	1 cut(s)	1293
BshFI	GGCC	7 cut(s)	104, 166, 516, 596, 659, 2104, 2141
BshNI	GGYRCC	1 cut(s)	1150
BsiHKAI	GWGCWC	3 cut(s)	1210, 2055, 2207
BsiHKCI	CYCGRG	2 cut(s)	129, 2109
BsiSI	CCGG	4 cut(s)	130, 167, 1433, 1755
BslI	CCNNNNNNNGG	8 cut(s)	58, 402, 700, 1283, 1794, 2492, 2553, 2594
BsmAI	GTCTC	3 cut(s)	276, 2460, 2766
BsmBI	CGTCTC	1 cut(s)	2460
BsmI	GAATGC	2 cut(s)	1228, 2608
BsnI	GGCC	7 cut(s)	104, 166, 516, 596, 659, 2104, 2141
Bso31I	GGTCTC	1 cut(s)	2766
BsoBI	CYCGRG	2 cut(s)	129, 2109
Bsp119I	TTCGAA	1 cut(s)	646
Bsp120I	GGGCCC	1 cut(s)	2139
Bsp1286I	GDGCHC	5 cut(s)	1210, 1839, 2055, 2143, 2207
Bsp13I	TCCGGA	1 cut(s)	1754
Bsp19I	CCATGG	1 cut(s)	2547
BspACI	CCGC	9 cut(s)	159, 244, 560, 563, 890, 1832, 2563, 2595, 2736
BspANI	GGCC	7 cut(s)	104, 166, 516, 596, 659, 2104, 2141
BspEI	TCCGGA	1 cut(s)	1754
BspFNI	CGCG	1 cut(s)	1293
BspHI	TCATGA	1 cut(s)	813
BspMAI	CTGCAG	5 cut(s)	48, 712, 880, 1503, 1781
BspMI	ACCTGC	1 cut(s)	2597
BspPI	GGATC	7 cut(s)	94, 838, 914, 927, 1503, 2500, 2523
BspQI	GCTCTTC	1 cut(s)	1494
BspT104I	TTCGAA	1 cut(s)	646
BspT107I	GGYRCC	1 cut(s)	1150
BspTI	CTTAAG	1 cut(s)	2627
BspTNI	GGTCTC	1 cut(s)	2766
BsrDI	GCAATG	5 cut(s)	153, 191, 448, 2365, 2691
BsrFI	RCCGGY	2 cut(s)	166, 1432
BsrI	ACTGG	4 cut(s)	201, 1241, 1545, 1923
BssAI	RCCGGY	2 cut(s)	166, 1432
BssHII	GCGCGC	1 cut(s)	1291
BssNI	GRCGYC	1 cut(s)	2453
BssT1I	CCWWGG	5 cut(s)	251, 613, 1739, 1890, 2547
Bst2UI	CCWGG	8 cut(s)	20, 53, 84, 397, 531, 1304, 1318, 2144
Bst4CI	ACNGT	6 cut(s)	121, 263, 487, 856, 1343, 1926
Bst6I	CTCTTC	2 cut(s)	315, 1494
BstACI	GRCGYC	1 cut(s)	2453
BstAFI	CTTAAG	1 cut(s)	2627
BstAPI	GCANNNNNTGC	2 cut(s)	725, 734
BstBI	TTCGAA	1 cut(s)	646
BstC8I	GCNNGC	7 cut(s)	27, 168, 1293, 1434, 1784, 2406, 2624
BstDSI	CCRYGG	2 cut(s)	327, 2547
BstENI	CCTNNNNNAGG	1 cut(s)	400
BstF5I	GGATG	3 cut(s)	463, 597, 1804
BstFNI	CGCG	1 cut(s)	1293
BstH2I	RGCGCY	1 cut(s)	2720
BstHHI	GCGC	4 cut(s)	1293, 1295, 2482, 2719
BstMAI	GTCTC	3 cut(s)	276, 2460, 2766
BstNI	CCWGG	8 cut(s)	20, 53, 84, 397, 531, 1304, 1318, 2144
BstNSI	RCATGY	1 cut(s)	2128
BstSFI	CTRYAG	5 cut(s)	44, 708, 876, 1499, 1777
BstSLI	GKGCMC	2 cut(s)	1210, 2143
BstUI	CGCG	1 cut(s)	1293
BstV2I	GAAGAC	4 cut(s)	499, 1079, 1620, 1737
BstX2I	RGATCY	8 cut(s)	86, 830, 906, 919, 1193, 1495, 2505, 2515
BstYI	RGATCY	8 cut(s)	86, 830, 906, 919, 1193, 1495, 2505, 2515
Bsu36I	CCTNAGG	3 cut(s)	99, 654, 1588
BsuRI	GGCC	7 cut(s)	104, 166, 516, 596, 659, 2104, 2141
BtgI	CCRYGG	2 cut(s)	327, 2547
BtrI	CACGTC	1 cut(s)	765
BtsCI	GGATG	3 cut(s)	463, 597, 1804
BtsI	GCAGTG	1 cut(s)	1230
BtsIMutI	CAGTG	3 cut(s)	423, 1230, 1931
BveI	ACCTGC	1 cut(s)	2597
Cac8I	GCNNGC	7 cut(s)	27, 168, 1293, 1434, 1784, 2406, 2624
CaiI	CAGNNNCTG	2 cut(s)	893, 1622
CciI	TCATGA	1 cut(s)	813
CfoI	GCGC	4 cut(s)	1293, 1295, 2482, 2719
Cfr10I	RCCGGY	2 cut(s)	166, 1432
Cfr13I	GGNCC	5 cut(s)	79, 103, 164, 2139, 2140
Cfr9I	CCCGGG	1 cut(s)	129
CseI	GACGC	1 cut(s)	2731
Csp6I	GTAC	3 cut(s)	1131, 1151, 2254
CviAII	CATG	7 cut(s)	814, 1370, 1789, 1808, 2125, 2189, 2548
CviQI	GTAC	3 cut(s)	1131, 1151, 2254
DriI	GACNNNNNGTC	1 cut(s)	1006
EaeI	YGGCCR	1 cut(s)	594
Eam1104I	CTCTTC	2 cut(s)	315, 1494
Eam1105I	GACNNNNNGTC	1 cut(s)	1006
EarI	CTCTTC	2 cut(s)	315, 1494
EciI	GGCGGA	1 cut(s)	2751
Eco130I	CCWWGG	5 cut(s)	251, 613, 1739, 1890, 2547
Eco24I	GRGCYC	2 cut(s)	1839, 2143
Eco31I	GGTCTC	1 cut(s)	2766
Eco32I	GATATC	1 cut(s)	2079
Eco47I	GGWCC	1 cut(s)	79
Eco47III	AGCGCT	1 cut(s)	2718
Eco57I	CTGAAG	1 cut(s)	1095
Eco81I	CCTNAGG	3 cut(s)	99, 654, 1588
Eco88I	CYCGRG	2 cut(s)	129, 2109
EcoNI	CCTNNNNNAGG	1 cut(s)	400
EcoO109I	RGGNCCY	3 cut(s)	103, 2139, 2140
EcoRII	CCWGG	8 cut(s)	18, 51, 82, 395, 529, 1302, 1316, 2142
EcoRV	GATATC	1 cut(s)	2079
EcoT14I	CCWWGG	5 cut(s)	251, 613, 1739, 1890, 2547
EcoT22I	ATGCAT	1 cut(s)	1423
EcoT38I	GRGCYC	2 cut(s)	1839, 2143
ErhI	CCWWGG	5 cut(s)	251, 613, 1739, 1890, 2547
Esp3I	CGTCTC	1 cut(s)	2460
FaeI	CATG	7 cut(s)	817, 1373, 1792, 1811, 2128, 2192, 2551
FalI	AAGNNNNNCTT	4 cut(s)	294, 326, 1093, 1125
FatI	CATG	7 cut(s)	813, 1369, 1788, 1807, 2124, 2188, 2547
FauI	CCCGC	1 cut(s)	2588
FblI	GTMKAC	1 cut(s)	1273
FokI	GGATG	3 cut(s)	470, 604, 1811
FriOI	GRGCYC	2 cut(s)	1839, 2143
FspBI	CTAG	1 cut(s)	2510
GlaI	GCGC	4 cut(s)	1292, 1294, 2481, 2718
GsuI	CTGGAG	3 cut(s)	35, 677, 1562
HaeII	RGCGCY	1 cut(s)	2720
HaeIII	GGCC	7 cut(s)	104, 166, 516, 596, 659, 2104, 2141
HapII	CCGG	4 cut(s)	130, 167, 1433, 1755
HgaI	GACGC	1 cut(s)	2731
HhaI	GCGC	4 cut(s)	1293, 1295, 2482, 2719
Hin1I	GRCGYC	1 cut(s)	2453
Hin1II	CATG	7 cut(s)	817, 1373, 1792, 1811, 2128, 2192, 2551
Hin6I	GCGC	4 cut(s)	1291, 1293, 2480, 2717
HinP1I	GCGC	4 cut(s)	1291, 1293, 2480, 2717
HincII	GTYRAC	1 cut(s)	1274
HindII	GTYRAC	1 cut(s)	1274
HindIII	AAGCTT	2 cut(s)	1865, 2630
HpaII	CCGG	4 cut(s)	130, 167, 1433, 1755
HphI	GGTGA	5 cut(s)	25, 109, 161, 1179, 2611
Hpy166II	GTNNAC	5 cut(s)	206, 826, 1208, 1274, 2451
Hpy8I	GTNNAC	5 cut(s)	206, 826, 1208, 1274, 2451
Hpy99I	CGWCG	3 cut(s)	415, 1275, 2478
HpyAV	CCTTC	8 cut(s)	50, 257, 279, 734, 1095, 1760, 2032, 2347
HpyCH4III	ACNGT	6 cut(s)	121, 263, 487, 856, 1343, 1926
HpyCH4IV	ACGT	2 cut(s)	764, 2453
HpySE526I	ACGT	2 cut(s)	764, 2453
Hsp92I	GRCGYC	1 cut(s)	2453
Hsp92II	CATG	7 cut(s)	817, 1373, 1792, 1811, 2128, 2192, 2551
HspAI	GCGC	4 cut(s)	1291, 1293, 2480, 2717
Kpn2I	TCCGGA	1 cut(s)	1754
KpnI	GGTACC	1 cut(s)	1154
KroI	GCCGGC	2 cut(s)	166, 1432
KroNI	GCCGGC	2 cut(s)	168, 1434
LguI	GCTCTTC	1 cut(s)	1494
LmnI	GCTCC	7 cut(s)	22, 54, 94, 696, 1741, 1834, 2210
LweI	GCATC	9 cut(s)	532, 715, 791, 1210, 1408, 1430, 1888, 1939, 2014
MaeI	CTAG	1 cut(s)	2510
MaeII	ACGT	2 cut(s)	764, 2453
MaeIII	GTNAC	9 cut(s)	121, 149, 481, 1105, 1185, 1329, 1551, 2003, 2113
MboII	GAAGA	7 cut(s)	302, 499, 1079, 1481, 1555, 1620, 1737
MflI	RGATCY	8 cut(s)	86, 830, 906, 919, 1193, 1495, 2505, 2515
MhlI	GDGCHC	5 cut(s)	1210, 1839, 2055, 2143, 2207
MlsI	TGGCCA	1 cut(s)	596
MluNI	TGGCCA	1 cut(s)	596
MlyI	GAGTC	7 cut(s)	389, 672, 872, 1193, 1250, 1604, 1634
MmeI	TCCRAC	2 cut(s)	57, 897
Mox20I	TGGCCA	1 cut(s)	596
Mph1103I	ATGCAT	1 cut(s)	1423
MroI	TCCGGA	1 cut(s)	1754
MroNI	GCCGGC	2 cut(s)	166, 1432
MroXI	GAANNNNTTC	1 cut(s)	198
MscI	TGGCCA	1 cut(s)	596
MseI	TTAA	4 cut(s)	699, 1200, 1980, 2628
MslI	CAYNNNNRTG	2 cut(s)	2399, 2769
Msp20I	TGGCCA	1 cut(s)	596
MspA1I	CMGCKG	2 cut(s)	890, 2563
MspCI	CTTAAG	1 cut(s)	2627
MspI	CCGG	4 cut(s)	130, 167, 1433, 1755
Mva1269I	GAATGC	2 cut(s)	1228, 2608
MvaI	CCWGG	8 cut(s)	20, 53, 84, 397, 531, 1304, 1318, 2144
MvnI	CGCG	1 cut(s)	1293
NaeI	GCCGGC	2 cut(s)	168, 1434
NciI	CCSGG	2 cut(s)	130, 131
NcoI	CCATGG	1 cut(s)	2547
NgoMIV	GCCGGC	2 cut(s)	166, 1432
NlaIII	CATG	7 cut(s)	817, 1373, 1792, 1811, 2128, 2192, 2551
NmeAIII	GCCGAG	1 cut(s)	2756
NmuCI	GTSAC	4 cut(s)	121, 149, 1185, 2003
NsiI	ATGCAT	1 cut(s)	1423
NspI	RCATGY	1 cut(s)	2128
NspV	TTCGAA	1 cut(s)	646
OliI	CACNNNNGTG	1 cut(s)	2399
PaeR7I	CTCGAG	1 cut(s)	2109
PagI	TCATGA	1 cut(s)	813
PauI	GCGCGC	1 cut(s)	1291
PciSI	GCTCTTC	1 cut(s)	1494
PctI	GAATGC	2 cut(s)	1228, 2608
PdiI	GCCGGC	2 cut(s)	168, 1434
PdmI	GAANNNNTTC	1 cut(s)	198
PfeI	GAWTC	9 cut(s)	177, 1037, 1046, 1476, 1506, 1715, 1815, 1904, 2228
PflMI	CCANNNNNTGG	2 cut(s)	2492, 2553
PfoI	TCCNGGA	1 cut(s)	51
PleI	GAGTC	7 cut(s)	388, 671, 871, 1192, 1250, 1603, 1633
PpsI	GAGTC	7 cut(s)	388, 671, 871, 1192, 1250, 1603, 1633
PsiI	TTATAA	1 cut(s)	572
Psp6I	CCWGG	8 cut(s)	18, 51, 82, 395, 529, 1302, 1316, 2142
PspGI	CCWGG	8 cut(s)	18, 51, 82, 395, 529, 1302, 1316, 2142
PspOMI	GGGCCC	1 cut(s)	2139
PspPI	GGNCC	5 cut(s)	79, 103, 164, 2139, 2140
PstI	CTGCAG	5 cut(s)	48, 712, 880, 1503, 1781
PstNI	CAGNNNCTG	2 cut(s)	893, 1622
PsuI	RGATCY	8 cut(s)	86, 830, 906, 919, 1193, 1495, 2505, 2515
PteI	GCGCGC	1 cut(s)	1291
RsaI	GTAC	3 cut(s)	1132, 1152, 2255
RsaNI	GTAC	3 cut(s)	1131, 1151, 2254
RseI	CAYNNNNRTG	2 cut(s)	2399, 2769
SalI	GTCGAC	1 cut(s)	1272
SapI	GCTCTTC	1 cut(s)	1494
SaqAI	TTAA	4 cut(s)	699, 1200, 1980, 2628
Sau96I	GGNCC	5 cut(s)	79, 103, 164, 2139, 2140
SchI	GAGTC	7 cut(s)	389, 672, 872, 1193, 1250, 1604, 1634
SduI	GDGCHC	5 cut(s)	1210, 1839, 2055, 2143, 2207
SfaNI	GCATC	9 cut(s)	532, 715, 791, 1210, 1408, 1430, 1888, 1939, 2014
SfcI	CTRYAG	5 cut(s)	44, 708, 876, 1499, 1777
Sfr274I	CTCGAG	1 cut(s)	2109
SfuI	TTCGAA	1 cut(s)	646
SinI	GGWCC	1 cut(s)	79
SlaI	CTCGAG	1 cut(s)	2109
SmaI	CCCGGG	1 cut(s)	131
SmiMI	CAYNNNNRTG	2 cut(s)	2399, 2769
SmlI	CTYRAG	5 cut(s)	476, 2048, 2060, 2109, 2627
SmoI	CTYRAG	5 cut(s)	476, 2048, 2060, 2109, 2627
SsiI	CCGC	9 cut(s)	159, 244, 560, 563, 890, 1832, 2563, 2595, 2736
SspI	AATATT	2 cut(s)	1560, 1570
SspMI	CTAG	1 cut(s)	2510
StyI	CCWWGG	5 cut(s)	251, 613, 1739, 1890, 2547
TaaI	ACNGT	6 cut(s)	121, 263, 487, 856, 1343, 1926
TaiI	ACGT	2 cut(s)	767, 2456
TatI	WGTACW	1 cut(s)	2253
TauI	GCSGC	4 cut(s)	161, 563, 566, 893
TfiI	GAWTC	9 cut(s)	177, 1037, 1046, 1476, 1506, 1715, 1815, 1904, 2228
Tru1I	TTAA	4 cut(s)	699, 1200, 1980, 2628
Tru9I	TTAA	4 cut(s)	699, 1200, 1980, 2628
TscAI	CASTG	3 cut(s)	430, 1230, 1931
TseFI	GTSAC	4 cut(s)	121, 149, 1185, 2003
Tsp45I	GTSAC	4 cut(s)	121, 149, 1185, 2003
TspGWI	ACGGA	4 cut(s)	223, 344, 843, 1832
TspMI	CCCGGG	1 cut(s)	129
TspRI	CASTG	3 cut(s)	430, 1230, 1931
Van91I	CCANNNNNTGG	2 cut(s)	2492, 2553
Vha464I	CTTAAG	1 cut(s)	2627
VneI	GTGCAC	1 cut(s)	1206
VpaK11BI	GGWCC	1 cut(s)	79
XagI	CCTNNNNNAGG	1 cut(s)	400
XapI	RAATTY	5 cut(s)	490, 976, 1308, 1463, 2260
XceI	RCATGY	1 cut(s)	2128
XhoI	CTCGAG	1 cut(s)	2109
XmaI	CCCGGG	1 cut(s)	129
XmiI	GTMKAC	1 cut(s)	1273
XmnI	GAANNNNTTC	1 cut(s)	198
XspI	CTAG	1 cut(s)	2510
ZraI	GACGTC	1 cut(s)	2454
Zsp2I	ATGCAT	1 cut(s)	1423

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.