Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7B

Orthogroup ID

OG0003250

Physical Location & Seq

Genomic Coordinates Reverse (-)

7592338 .. 7598056

Gene Structure 5719 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7BG101800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2790 bp

ATGATGGCAAAACAGGAGCAAAAGCAGGAGCAGCCTGCAATCATCAACCCTCTGCAGGTCCAGGAAGACGGGGATCTGGACCCTGGATCTGACGACCTCGAGGCTCCTCTGCCTCTCACCGTCACTTCCCGGGTCTTGTATATGTTGGGTGACATTGCGTCTGGACCGGCCTTCAAAATCACTCAATGGCTGGAATTGGTCCGTAAACGCAGTGCCAAATACCATTCCTCTGGCTTCCCTCGCCGCCCATTAAGGCTTGACACAATGCCTGCTAGTGCAGGAGAGTCTAGTGAGGACTGGAAAGGTCCTGCGTCGCCTGAGCAAACCACAGAAGTCAATCTCTGGGAAAGACTTGGTAAAGCTGCTACATTGGACATTGAGTCGAGTTTTTTCTCATGGGATAGGCTTTCTTCACTTCACCACACTGAACATAGTAGTAGCAATGATCAGTCTGAGGATGAAATGAACAAAGCGCTGGAGGTCACCGTGAATTCGGGGGGAGTTGTTTTCTTTGCCCTTTTCAACCAGCCAGGGACTGATGATAACTCTTCTAAGGAAGCAGCGGCTGTTATAAAGATATCCTCATCGAGGATGGCCACACAGTCTGAACGTCTAGGTTACGAATTTGCAAAGTGGCTTGGAGTTCGAACTCCACAGGCGAGAGTCATACATAATTGTAGTATGGAGTGGCTCCAGATGAAGGAAGCAGCAGAAAAAGCAAGGGACACAGCAGTTTCAGAAGGAGATGAATGTGGGGAAATGACATGTTCGGAACTTTTGGAAGCTCTTGAACTTAGCCGATGCCTTTTGCTCATGAGCTATGTTCACGGATCTCCTTTACTTGAAAGCTCAAGTGTATTTGAGGTGAAGGAAACTGCAGAGAAAACAGCAGCTTCTCTTGGTAGGATCTTGATGTTGGACCTTGTCATCAGAAATGAAGATAGACTACCTTGCCGTCAGCTCAGATGGCGTGGAAACTCGGCAAATCTATTGTTTGCAGACAAAATGGATTTTGGAAATATGGACAGATTGGAGGAAGCATTTGATTCTGCCATCAAGAGATACAAACCAAGAGTGATCAGAGCTCTTCAAAAGGATAGAAGGGCAACTTCAGTAGATATCAGATTGCATAATACAGCATTGGTACCACAGAAATCTGATCTTTCTGACATTATGGATTCGCCAAGGTCTAGTCAGATGAGTGTAAAGAGTCAACTCTCAGATGATTTAATGTTTTCCGATTTTCCAGTTGTGGCTATTGATTCTGGCGTTCCTCGTCGACCTCCTGCTGGAAAACGTGCAAATGACCAGGAAATCTATCCAAAGTTGGTTGAGTTACTACTGAACAATTCTGAATACTCCTCTAATCTGTTACATGACATAACATTAGGGAAATTAGGACGTCCTCCGTTACAAGATACTGATGCATCTGATACACAAGCAATGGAAATGACTTCGGTTGTTCAAGGGTTCCGTAGTGGGTTCAAATGTGCTCTCAGGGATCTGCAGGGATTCCATATCTTCTTACTCACACTTCATCAAAAACTGGAGAATTTGATACGTATATTTTTCAATATTATAGATAAAATAGGGGAGTCTGATAAAGAGGATTTGGTAGTTACTGAGTCTCCATCACAAGCTGCCGGAAGTGGTTATCTTCCTTCTACACCAAGTAAGGAGAGGCTTGTCAATGAGAACCATTCAGATTTTAGTGATTCCGAATTGCAGAGAACTGCTCCAAGGCCTTCAACTTCAGGACTTAAAGAAAGCTGTGACTGCAGCTCTCCTATGTCACGGGAGAGTTGGCACGGAAAGTTTTGGAAAGGGAGTGCAAGTTCAGATCCCCTCCGTAGTCTGCGTTTGACAGCAAAGCTCCGGGACTTTCATAAAAATGCCAAGGTCGATGCTGAAACGAACAAAGAATTGGAACAGTGGAATGAAATGCTAAAGAGCGATGCAGTCAAACTTTGCCAGGAGAACAATTTTAATGCTGGGTTCTTTGAGGGTAGTGACAATAACGGCGTAGTTGACGCTTATGAATTGAAGGTCAGACTTGAGCACATTCTCGAGAGAATTTCATTGATATCTGAGGCTGCAAATACAGAGAGGCCATCTCGAATCACAAGTAGCCTGTTTATTGGTGGAGCGCTTGCTGCACGATCTGTATACACACTGCAACGCTTAGGAATCACTCATATATTATGTTTGTGTTCCAATGAAATTGGACAGTCAGATTCTCAGTTTCCTGATCTATTTGAGTACAAAAACTTCTCTATATGTGACAGTGAAGATTTCAACATCAGCAACATCTTTGATGAAGCTGTTGAGTTTATTGATCGTGTTGAAGAAACAGGAGGGAAGGTTCTAGTCCATTGCTTTGAGGGAAAAAGTAGAAGTGCTACACTAGTGCTTGCATACCTAATGCTCAGAAAGAATTCCACTCTATTAGAAGCATGGAATTCTCTAAAGCAGGTTCATCGCCGAGCACAACCCAACGATGGGTTTGCGAAGATCTTGTTGAATTTGGATAAGAAACTGCATGGTAAAGTTTCAATGGAGTGGCATCAGCGGAAGCCAATGATGAAAGTTTGCCCCATCTGTGGGGTGAATGCGGGTCTGAGTAGCAGCTCTCTTAAGCTTCATTTACAGAAATCACATAAGAAACTATCGTCTGGTAGCGTGGATAGCGCAATGACAATGGAAATACAAAAGGCTTTGACAGTACTAAAAATCAGTCGAGGTGGAAGTGTCAGCCCCACACAAAGATCTCATTCAGATGTTGAAGAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000226 GO:0001101 GO:0003674 GO:0003824 GO:0004721 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007010 GO:0007017 GO:0008138 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009987 GO:0010033 GO:0010119 GO:0010468 GO:0016043 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019222 GO:0019538 GO:0030865 GO:0031122 GO:0033993 GO:0036211 GO:0042221 GO:0042578 GO:0043170 GO:0043412 GO:0043622 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0060255 GO:0065007 GO:0071704 GO:0071840 GO:0097305 GO:0097435 GO:0140096 GO:1901564 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Act-Frag_cataly DSPc

Protein Analysis

929

Amino Acids

103.72

Weight (kDa)

5.77

Isoelectric Point (pI)

52.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Act-Frag_cataly	PF09192	111 - 424	1e-46	Actin-fragmin kinase, catalytic
DSPc	PF00782	713 - 843	1.3e-24	Dual specificity phosphatase, catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0003250)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G23720 AT5G23720 AT5G23720
fragaria_vesca	FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220 FvH4_5g09220
malus_domestica	MD06G1132400.v1.1 MD14G1148800.v1.1
prunus_persica	Prupe.5G143400_v2.0.a1
pyrus_communis	pycom06g12400 pycom14g12360
rosa_chinensis	RchiOBHm_Chr4g0416471 RchiOBHm_Chr7g0188941 RchiOBHm_Chr7g0194331
rosa_laevigata	RLG00000004049 RLG00000004648
rosa_multiflora	Rmu_co8071116.1_g000001 Rmu_sc0007831.1_g000001 Rmu_sc0032883.1_g000001 Rmu_ssc0000113.1_g000006
rosa_roxburghii	Rroxscaffold_3G00265920
rosa_rugosa	Rorug06G0494800 Rorug06G0494900 Rorug06G0495000
rosa_samantha	Rh7AG099700 Rh7BG101800 Rh7CG103100 Rh7CG146600 Rh7DG101600 Rh7DG144200
rosa_wichuraiana	Rw7G008590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	572
AatII	GACGTC	1 cut(s)	1405
AbsI	CCTCGAGG	1 cut(s)	98
Acc36I	ACCTGC	2 cut(s)	46, 2461
Acc65I	GGTACC	1 cut(s)	1144
AccB1I	GGYRCC	1 cut(s)	1144
AccI	GTMKAC	2 cut(s)	1279, 2166
AciI	CCGC	4 cut(s)	244, 563, 2569, 2612
AclWI	GGATC	6 cut(s)	81, 94, 838, 914, 1509, 1835
AcoI	YGGCCR	1 cut(s)	594
AcsI	RAATTY	7 cut(s)	490, 623, 1552, 2073, 2434, 2458, 2521
AcuI	CTGAAG	2 cut(s)	1095, 1737
AcyI	GRCGYC	1 cut(s)	1402
AfaI	GTAC	3 cut(s)	1146, 2261, 2724
AfeI	AGCGCT	2 cut(s)	474, 2148
AfiI	CCNNNNNNNGG	8 cut(s)	55, 588, 700, 1289, 2498, 2499, 2600, 2601
AflII	CTTAAG	1 cut(s)	2633
AflIII	ACRYGT	1 cut(s)	764
AhdI	GACNNNNNGTC	2 cut(s)	157, 379
AhlI	ACTAGT	1 cut(s)	2404
AjnI	CCWGG	5 cut(s)	60, 82, 529, 1308, 1971
AjuI	GAANNNNNNNTTGG	2 cut(s)	1907, 1939
AloI	GAACNNNNNNTCC	4 cut(s)	1819, 1851, 2346, 2378
Alw21I	GWGCWC	4 cut(s)	1087, 1495, 2061, 2488
Alw26I	GTCTC	1 cut(s)	1632
AlwI	GGATC	6 cut(s)	81, 94, 838, 914, 1509, 1835
AlwNI	CAGNNNCTG	2 cut(s)	536, 566
Ama87I	CYCGRG	3 cut(s)	98, 129, 2066
Aor51HI	AGCGCT	2 cut(s)	474, 2148
AoxI	GGCC	4 cut(s)	168, 594, 1742, 2108
ApoI	RAATTY	7 cut(s)	490, 623, 1552, 2073, 2434, 2458, 2521
Asp700I	GAANNNNTTC	1 cut(s)	2434
Asp718I	GGTACC	1 cut(s)	1144
AspLEI	GCGC	3 cut(s)	475, 2149, 2690
AspS9I	GGNCC	6 cut(s)	58, 79, 164, 199, 305, 919
AsuC2I	CCSGG	3 cut(s)	130, 131, 1877
AsuHPI	GGTGA	6 cut(s)	109, 161, 410, 475, 877, 2617
AsuII	TTCGAA	1 cut(s)	646
AvaI	CYCGRG	3 cut(s)	98, 129, 2066
AvaII	GGWCC	6 cut(s)	58, 79, 164, 199, 305, 919
BalI	TGGCCA	1 cut(s)	596
BanI	GGYRCC	1 cut(s)	1144
BanII	GRGCYC	1 cut(s)	1087
BarI	GAAGNNNNNNTAC	4 cut(s)	930, 962, 2252, 2284
BbsI	GAAGAC	1 cut(s)	72
Bbv12I	GWGCWC	4 cut(s)	1087, 1495, 2061, 2488
BccI	CCATC	7 cut(s)	586, 960, 1061, 1639, 2119, 2492, 2603
BceAI	ACGGC	2 cut(s)	939, 2035
BcgI	CGANNNNNNTGC	4 cut(s)	2459, 2486, 2493, 2520
BciT130I	CCWGG	5 cut(s)	62, 84, 531, 1310, 1973
BclI	TGATCA	2 cut(s)	445, 1077
BcnI	CCSGG	3 cut(s)	130, 131, 1877
BcoDI	GTCTC	1 cut(s)	1632
BcuI	ACTAGT	1 cut(s)	2404
BfaI	CTAG	6 cut(s)	273, 288, 614, 1191, 2366, 2405
BfmI	CTRYAG	4 cut(s)	53, 876, 1505, 1777
BfoI	RGCGCY	2 cut(s)	476, 2150
BfrI	CTTAAG	1 cut(s)	2633
BfuAI	ACCTGC	2 cut(s)	46, 2461
BglII	AGATCT	2 cut(s)	2511, 2765
BmcAI	AGTACT	1 cut(s)	2724
Bme1390I	CCNGG	8 cut(s)	62, 84, 130, 131, 531, 1310, 1877, 1973
Bme18I	GGWCC	6 cut(s)	58, 79, 164, 199, 305, 919
BmeRI	GACNNNNNGTC	2 cut(s)	157, 379
BmeT110I	CYCGRG	3 cut(s)	98, 129, 2066
BmgT120I	GGNCC	6 cut(s)	58, 79, 164, 199, 305, 919
BmiI	GGNNCC	5 cut(s)	81, 105, 692, 1146, 1472
BmrFI	CCNGG	8 cut(s)	62, 84, 130, 131, 531, 1310, 1877, 1973
BmsI	GCATC	6 cut(s)	791, 1414, 1436, 1894, 1945, 2572
BpiI	GAAGAC	1 cut(s)	72
BplI	GAGNNNNNCTC	4 cut(s)	1720, 1752, 2098, 2130
BpmI	CTGGAG	3 cut(s)	497, 677, 1568
Bpu10I	CCTNAGC	2 cut(s)	318, 2182
Bpu14I	TTCGAA	1 cut(s)	646
BpuEI	CTTGAG	2 cut(s)	835, 2075
BpuMI	CCSGG	3 cut(s)	130, 131, 1877
BsaAI	YACGTR	1 cut(s)	1562
BsaHI	GRCGYC	1 cut(s)	1402
BsaJI	CCNNGG	6 cut(s)	82, 129, 530, 1184, 1739, 1896
BsaXI	ACNNNNNCTCC	6 cut(s)	1612, 1642, 1819, 1849, 2346, 2376
Bsc4I	CCNNNNNNNGG	8 cut(s)	55, 588, 700, 1289, 2498, 2499, 2600, 2601
Bse118I	RCCGGY	1 cut(s)	166
Bse1I	ACTGG	3 cut(s)	302, 1247, 1551
Bse3DI	GCAATG	5 cut(s)	153, 448, 1449, 2371, 2697
BseBI	CCWGG	5 cut(s)	62, 84, 531, 1310, 1973
BseDI	CCNNGG	6 cut(s)	82, 129, 530, 1184, 1739, 1896
BseGI	GGATG	2 cut(s)	463, 597
BseLI	CCNNNNNNNGG	8 cut(s)	55, 588, 700, 1289, 2498, 2499, 2600, 2601
BseMI	GCAATG	5 cut(s)	153, 448, 1449, 2371, 2697
BseNI	ACTGG	3 cut(s)	302, 1247, 1551
BseRI	GAGGAG	2 cut(s)	96, 1351
BseYI	CCCAGC	1 cut(s)	1991
BsgI	GTGCAG	2 cut(s)	297, 2139
BshFI	GGCC	4 cut(s)	170, 596, 1744, 2110
BshNI	GGYRCC	1 cut(s)	1144
BsiHKAI	GWGCWC	4 cut(s)	1087, 1495, 2061, 2488
BsiHKCI	CYCGRG	3 cut(s)	98, 129, 2066
BsiSI	CCGG	4 cut(s)	130, 167, 1644, 1876
BslFI	GGGAC	3 cut(s)	547, 737, 1892
BslI	CCNNNNNNNGG	8 cut(s)	55, 588, 700, 1289, 2498, 2499, 2600, 2601
BsmAI	GTCTC	1 cut(s)	1632
BsmFI	GGGAC	3 cut(s)	547, 737, 1892
BsmI	GAATGC	1 cut(s)	2614
BsnI	GGCC	4 cut(s)	170, 596, 1744, 2110
BsoBI	CYCGRG	3 cut(s)	98, 129, 2066
Bsp119I	TTCGAA	1 cut(s)	646
Bsp1286I	GDGCHC	4 cut(s)	1087, 1495, 2061, 2488
BspACI	CCGC	4 cut(s)	244, 563, 2569, 2612
BspANI	GGCC	4 cut(s)	170, 596, 1744, 2110
BspHI	TCATGA	1 cut(s)	813
BspLI	GGNNCC	5 cut(s)	81, 105, 692, 1146, 1472
BspMAI	CTGCAG	4 cut(s)	57, 880, 1509, 1781
BspMI	ACCTGC	2 cut(s)	46, 2461
BspPI	GGATC	6 cut(s)	81, 94, 838, 914, 1509, 1835
BspQI	GCTCTTC	1 cut(s)	1092
BspT104I	TTCGAA	1 cut(s)	646
BspT107I	GGYRCC	1 cut(s)	1144
BspTI	CTTAAG	1 cut(s)	2633
BsrDI	GCAATG	5 cut(s)	153, 448, 1449, 2371, 2697
BsrFI	RCCGGY	1 cut(s)	166
BsrI	ACTGG	3 cut(s)	302, 1247, 1551
BssAI	RCCGGY	1 cut(s)	166
BssECI	CCNNGG	6 cut(s)	82, 129, 530, 1184, 1739, 1896
BssNAI	GTATAC	1 cut(s)	2167
BssNI	GRCGYC	1 cut(s)	1402
BssT1I	CCWWGG	3 cut(s)	1184, 1739, 1896
Bst1107I	GTATAC	1 cut(s)	2167
Bst2UI	CCWGG	5 cut(s)	62, 84, 531, 1310, 1973
Bst4CI	ACNGT	7 cut(s)	121, 487, 603, 1932, 2229, 2285, 2722
Bst6I	CTCTTC	3 cut(s)	553, 1092, 2778
BstACI	GRCGYC	1 cut(s)	1402
BstAFI	CTTAAG	1 cut(s)	2633
BstBAI	YACGTR	1 cut(s)	1562
BstBI	TTCGAA	1 cut(s)	646
BstC8I	GCNNGC	4 cut(s)	36, 270, 2151, 2412
BstEII	GGTNACC	1 cut(s)	481
BstENI	CCTNNNNNAGG	1 cut(s)	586
BstF5I	GGATG	2 cut(s)	463, 597
BstH2I	RGCGCY	2 cut(s)	476, 2150
BstHHI	GCGC	3 cut(s)	475, 2149, 2690
BstMAI	GTCTC	1 cut(s)	1632
BstMWI	GCNNNNNNNGC	7 cut(s)	31, 240, 716, 967, 1776, 2153, 2685
BstNI	CCWGG	5 cut(s)	62, 84, 531, 1310, 1973
BstNSI	RCATGY	1 cut(s)	768
BstPI	GGTNACC	1 cut(s)	481
BstSCI	CCNGG	8 cut(s)	60, 82, 128, 129, 529, 1308, 1875, 1971
BstSFI	CTRYAG	4 cut(s)	53, 876, 1505, 1777
BstSNI	TACGTA	1 cut(s)	1562
BstV2I	GAAGAC	1 cut(s)	72
BstX2I	RGATCY	8 cut(s)	73, 86, 830, 906, 1501, 1840, 2511, 2765
BstXI	CCANNNNNNTGG	1 cut(s)	230
BstYI	RGATCY	8 cut(s)	73, 86, 830, 906, 1501, 1840, 2511, 2765
BstZ17I	GTATAC	1 cut(s)	2167
BsuRI	GGCC	4 cut(s)	170, 596, 1744, 2110
BtgZI	GCGATG	2 cut(s)	1968, 2462
BtsCI	GGATG	2 cut(s)	463, 597
BtsI	GCAGTG	2 cut(s)	217, 2171
BtsIMutI	CAGTG	5 cut(s)	217, 423, 1937, 2171, 2290
BveI	ACCTGC	2 cut(s)	46, 2461
Cac8I	GCNNGC	4 cut(s)	36, 270, 2151, 2412
CaiI	CAGNNNCTG	2 cut(s)	536, 566
CciI	TCATGA	1 cut(s)	813
CfoI	GCGC	3 cut(s)	475, 2149, 2690
Cfr10I	RCCGGY	1 cut(s)	166
Cfr13I	GGNCC	6 cut(s)	58, 79, 164, 199, 305, 919
Cfr9I	CCCGGG	1 cut(s)	129
CseI	GACGC	3 cut(s)	147, 300, 2039
Csp6I	GTAC	3 cut(s)	1145, 2260, 2723
CviAII	CATG	6 cut(s)	396, 765, 814, 1376, 2454, 2540
CviQI	GTAC	3 cut(s)	1145, 2260, 2723
DriI	GACNNNNNGTC	2 cut(s)	157, 379
EaeI	YGGCCR	1 cut(s)	594
Eam1104I	CTCTTC	3 cut(s)	553, 1092, 2778
Eam1105I	GACNNNNNGTC	2 cut(s)	157, 379
EarI	CTCTTC	3 cut(s)	553, 1092, 2778
Ecl136II	GAGCTC	1 cut(s)	1085
Eco105I	TACGTA	1 cut(s)	1562
Eco130I	CCWWGG	3 cut(s)	1184, 1739, 1896
Eco147I	AGGCCT	1 cut(s)	1744
Eco24I	GRGCYC	1 cut(s)	1087
Eco32I	GATATC	3 cut(s)	579, 1120, 2085
Eco47I	GGWCC	6 cut(s)	58, 79, 164, 199, 305, 919
Eco47III	AGCGCT	2 cut(s)	474, 2148
Eco53kI	GAGCTC	1 cut(s)	1085
Eco57I	CTGAAG	2 cut(s)	1095, 1737
Eco88I	CYCGRG	3 cut(s)	98, 129, 2066
Eco91I	GGTNACC	1 cut(s)	481
EcoICRI	GAGCTC	1 cut(s)	1085
EcoNI	CCTNNNNNAGG	1 cut(s)	586
EcoO109I	RGGNCCY	1 cut(s)	305
EcoO65I	GGTNACC	1 cut(s)	481
EcoRI	GAATTC	3 cut(s)	490, 2434, 2458
EcoRII	CCWGG	5 cut(s)	60, 82, 529, 1308, 1971
EcoRV	GATATC	3 cut(s)	579, 1120, 2085
EcoT14I	CCWWGG	3 cut(s)	1184, 1739, 1896
EcoT22I	ATGCAT	1 cut(s)	1429
EcoT38I	GRGCYC	1 cut(s)	1087
ErhI	CCWWGG	3 cut(s)	1184, 1739, 1896
FaeI	CATG	6 cut(s)	399, 768, 817, 1379, 2457, 2543
FalI	AAGNNNNNCTT	2 cut(s)	1093, 1125
FaqI	GGGAC	3 cut(s)	547, 737, 1892
FatI	CATG	6 cut(s)	395, 764, 813, 1375, 2453, 2539
FauI	CCCGC	1 cut(s)	2605
FbaI	TGATCA	2 cut(s)	445, 1077
FblI	GTMKAC	2 cut(s)	1279, 2166
FokI	GGATG	2 cut(s)	470, 604
FriOI	GRGCYC	1 cut(s)	1087
FspBI	CTAG	6 cut(s)	273, 288, 614, 1191, 2366, 2405
GlaI	GCGC	3 cut(s)	474, 2148, 2689
GsaI	CCCAGC	1 cut(s)	1995
GsuI	CTGGAG	3 cut(s)	497, 677, 1568
HaeII	RGCGCY	2 cut(s)	476, 2150
HaeIII	GGCC	4 cut(s)	170, 596, 1744, 2110
HapII	CCGG	4 cut(s)	130, 167, 1644, 1876
HgaI	GACGC	3 cut(s)	147, 300, 2039
HhaI	GCGC	3 cut(s)	475, 2149, 2690
Hin1I	GRCGYC	1 cut(s)	1402
Hin1II	CATG	6 cut(s)	399, 768, 817, 1379, 2457, 2543
Hin6I	GCGC	3 cut(s)	473, 2147, 2688
HinP1I	GCGC	3 cut(s)	473, 2147, 2688
HincII	GTYRAC	3 cut(s)	1214, 1280, 2029
HindII	GTYRAC	3 cut(s)	1214, 1280, 2029
HindIII	AAGCTT	1 cut(s)	2636
HpaII	CCGG	4 cut(s)	130, 167, 1644, 1876
HphI	GGTGA	6 cut(s)	109, 161, 410, 475, 877, 2617
Hpy166II	GTNNAC	6 cut(s)	206, 826, 1214, 1280, 2029, 2167
Hpy8I	GTNNAC	6 cut(s)	206, 826, 1214, 1280, 2029, 2167
Hpy99I	CGWCG	2 cut(s)	316, 1281
HpyAV	CCTTC	9 cut(s)	181, 694, 734, 862, 1095, 1671, 1755, 2038, 2353
HpyCH4III	ACNGT	7 cut(s)	121, 487, 603, 1932, 2229, 2285, 2722
HpyCH4IV	ACGT	4 cut(s)	610, 1297, 1402, 1561
HpyF10VI	GCNNNNNNNGC	7 cut(s)	31, 240, 716, 967, 1776, 2153, 2685
HpySE526I	ACGT	4 cut(s)	610, 1297, 1402, 1561
Hsp92I	GRCGYC	1 cut(s)	1402
Hsp92II	CATG	6 cut(s)	399, 768, 817, 1379, 2457, 2543
HspAI	GCGC	3 cut(s)	473, 2147, 2688
KpnI	GGTACC	1 cut(s)	1148
Ksp22I	TGATCA	2 cut(s)	445, 1077
LguI	GCTCTTC	1 cut(s)	1092
LmnI	GCTCC	7 cut(s)	16, 28, 109, 696, 1741, 1878, 2144
LweI	GCATC	6 cut(s)	791, 1414, 1436, 1894, 1945, 2572
MaeI	CTAG	6 cut(s)	273, 288, 614, 1191, 2366, 2405
MaeII	ACGT	4 cut(s)	610, 1297, 1402, 1561
MflI	RGATCY	8 cut(s)	73, 86, 830, 906, 1501, 1840, 2511, 2765
MhlI	GDGCHC	4 cut(s)	1087, 1495, 2061, 2488
MlsI	TGGCCA	1 cut(s)	596
MluNI	TGGCCA	1 cut(s)	596
MlyI	GAGTC	6 cut(s)	293, 389, 672, 1219, 1604, 1634
MmeI	TCCRAC	1 cut(s)	897
Mox20I	TGGCCA	1 cut(s)	596
Mph1103I	ATGCAT	1 cut(s)	1429
MroXI	GAANNNNTTC	1 cut(s)	2434
MscI	TGGCCA	1 cut(s)	596
MseI	TTAA	5 cut(s)	251, 1229, 1761, 1986, 2634
MslI	CAYNNNNRTG	2 cut(s)	1890, 2775
Msp20I	TGGCCA	1 cut(s)	596
MspA1I	CMGCKG	2 cut(s)	563, 2569
MspCI	CTTAAG	1 cut(s)	2633
MspI	CCGG	4 cut(s)	130, 167, 1644, 1876
MspR9I	CCNGG	8 cut(s)	62, 84, 130, 131, 531, 1310, 1877, 1973
Mva1269I	GAATGC	1 cut(s)	2614
MvaI	CCWGG	5 cut(s)	62, 84, 531, 1310, 1973
MwoI	GCNNNNNNNGC	7 cut(s)	31, 240, 716, 967, 1776, 2153, 2685
NciI	CCSGG	3 cut(s)	130, 131, 1877
NlaIII	CATG	6 cut(s)	399, 768, 817, 1379, 2457, 2543
NlaIV	GGNNCC	5 cut(s)	81, 105, 692, 1146, 1472
NmeAIII	GCCGAG	2 cut(s)	959, 2507
NmuCI	GTSAC	7 cut(s)	121, 149, 481, 1772, 1791, 2009, 2279
NsiI	ATGCAT	1 cut(s)	1429
NspI	RCATGY	1 cut(s)	768
NspV	TTCGAA	1 cut(s)	646
PaeR7I	CTCGAG	2 cut(s)	98, 2066
PagI	TCATGA	1 cut(s)	813
PceI	AGGCCT	1 cut(s)	1744
PciI	ACATGT	1 cut(s)	764
PciSI	GCTCTTC	1 cut(s)	1092
PctI	GAATGC	1 cut(s)	2614
PdmI	GAANNNNTTC	1 cut(s)	2434
PfeI	GAWTC	8 cut(s)	1046, 1178, 1262, 1512, 1715, 2118, 2187, 2234
PflFI	GACNNNGTC	1 cut(s)	923
PfoI	TCCNGGA	2 cut(s)	60, 1875
PleI	GAGTC	6 cut(s)	292, 388, 671, 1218, 1603, 1633
PpsI	GAGTC	6 cut(s)	292, 388, 671, 1218, 1603, 1633
Ppu21I	YACGTR	1 cut(s)	1562
PpuMI	RGGWCCY	1 cut(s)	305
PscI	ACATGT	1 cut(s)	764
PsiI	TTATAA	1 cut(s)	572
Psp124BI	GAGCTC	1 cut(s)	1087
Psp5II	RGGWCCY	1 cut(s)	305
Psp6I	CCWGG	5 cut(s)	60, 82, 529, 1308, 1971
PspEI	GGTNACC	1 cut(s)	481
PspFI	CCCAGC	1 cut(s)	1991
PspGI	CCWGG	5 cut(s)	60, 82, 529, 1308, 1971
PspN4I	GGNNCC	5 cut(s)	81, 105, 692, 1146, 1472
PspPI	GGNCC	6 cut(s)	58, 79, 164, 199, 305, 919
PspPPI	RGGWCCY	1 cut(s)	305
PspXI	VCTCGAGB	1 cut(s)	98
PstI	CTGCAG	4 cut(s)	57, 880, 1509, 1781
PstNI	CAGNNNCTG	2 cut(s)	536, 566
PsuI	RGATCY	8 cut(s)	73, 86, 830, 906, 1501, 1840, 2511, 2765
PsyI	GACNNNGTC	1 cut(s)	923
RsaI	GTAC	3 cut(s)	1146, 2261, 2724
RsaNI	GTAC	3 cut(s)	1145, 2260, 2723
RseI	CAYNNNNRTG	2 cut(s)	1890, 2775
SacI	GAGCTC	1 cut(s)	1087
SalI	GTCGAC	1 cut(s)	1278
SapI	GCTCTTC	1 cut(s)	1092
SaqAI	TTAA	5 cut(s)	251, 1229, 1761, 1986, 2634
Sau96I	GGNCC	6 cut(s)	58, 79, 164, 199, 305, 919
ScaI	AGTACT	1 cut(s)	2724
SchI	GAGTC	6 cut(s)	293, 389, 672, 1219, 1604, 1634
ScrFI	CCNGG	8 cut(s)	62, 84, 130, 131, 531, 1310, 1877, 1973
SduI	GDGCHC	4 cut(s)	1087, 1495, 2061, 2488
SfaNI	GCATC	6 cut(s)	791, 1414, 1436, 1894, 1945, 2572
SfcI	CTRYAG	4 cut(s)	53, 876, 1505, 1777
Sfr274I	CTCGAG	2 cut(s)	98, 2066
SfuI	TTCGAA	1 cut(s)	646
SinI	GGWCC	6 cut(s)	58, 79, 164, 199, 305, 919
SlaI	CTCGAG	2 cut(s)	98, 2066
SmaI	CCCGGG	1 cut(s)	131
SmiMI	CAYNNNNRTG	2 cut(s)	1890, 2775
SmlI	CTYRAG	5 cut(s)	98, 850, 2054, 2066, 2633
SmoI	CTYRAG	5 cut(s)	98, 850, 2054, 2066, 2633
SnaBI	TACGTA	1 cut(s)	1562
SpeI	ACTAGT	1 cut(s)	2404
SseBI	AGGCCT	1 cut(s)	1744
SsiI	CCGC	4 cut(s)	244, 563, 2569, 2612
SspI	AATATT	1 cut(s)	1576
SspMI	CTAG	6 cut(s)	273, 288, 614, 1191, 2366, 2405
SstI	GAGCTC	1 cut(s)	1087
StuI	AGGCCT	1 cut(s)	1744
StyD4I	CCNGG	8 cut(s)	60, 82, 128, 129, 529, 1308, 1875, 1971
StyI	CCWWGG	3 cut(s)	1184, 1739, 1896
TaaI	ACNGT	7 cut(s)	121, 487, 603, 1932, 2229, 2285, 2722
TaiI	ACGT	4 cut(s)	613, 1300, 1405, 1564
TaqI	TCGA	9 cut(s)	99, 383, 587, 646, 1279, 1902, 2067, 2116, 2737
TatI	WGTACW	2 cut(s)	2259, 2722
TauI	GCSGC	2 cut(s)	246, 566
TfiI	GAWTC	8 cut(s)	1046, 1178, 1262, 1512, 1715, 2118, 2187, 2234
Tru1I	TTAA	5 cut(s)	251, 1229, 1761, 1986, 2634
Tru9I	TTAA	5 cut(s)	251, 1229, 1761, 1986, 2634
TscAI	CASTG	5 cut(s)	217, 430, 1937, 2178, 2290
TseFI	GTSAC	7 cut(s)	121, 149, 481, 1772, 1791, 2009, 2279
Tsp45I	GTSAC	7 cut(s)	121, 149, 481, 1772, 1791, 2009, 2279
TspGWI	ACGGA	6 cut(s)	191, 843, 1398, 1463, 1824, 1838
TspMI	CCCGGG	1 cut(s)	129
TspRI	CASTG	5 cut(s)	217, 430, 1937, 2178, 2290
Tth111I	GACNNNGTC	1 cut(s)	923
Vha464I	CTTAAG	1 cut(s)	2633
VpaK11BI	GGWCC	6 cut(s)	58, 79, 164, 199, 305, 919
XagI	CCTNNNNNAGG	1 cut(s)	586
XapI	RAATTY	7 cut(s)	490, 623, 1552, 2073, 2434, 2458, 2521
XceI	RCATGY	1 cut(s)	768
XhoI	CTCGAG	2 cut(s)	98, 2066
XmaI	CCCGGG	1 cut(s)	129
XmiI	GTMKAC	2 cut(s)	1279, 2166
XmnI	GAANNNNTTC	1 cut(s)	2434
XspI	CTAG	6 cut(s)	273, 288, 614, 1191, 2366, 2405
ZraI	GACGTC	1 cut(s)	1403
ZrmI	AGTACT	1 cut(s)	2724
Zsp2I	ATGCAT	1 cut(s)	1429

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.