AT5G23950

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0004046
Physical Location & Seq
Forward (+)
8082650 .. 8083772
1123 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G23950.2

Sequence Viewer

Length: 660 bp
ATGTCATCCCACGGCCGAGAAGTAGAGGTGACCATCTCATCGGCAAAGGATATCAAGAACGTGAACTGGCGCAACGGCCCAAACAAGCCATACGCCGTTGTATGGATCGATCCGAAGTTCAAATCCTCCACAAGAGTTGACGAAGACGGCAACACTTGTACAACGTGGAACGAGACATTTGTCATCGCTTTACCTCCGGCCAACGACGATGACGACAAGGTATACATCAACATCGTACACGCAGGCCGTGAAGAGAACACAAAGCCCCTCATCGGCTCCGCCCATCTCAGCCTCCGTGATGTCATTGATGACGTCGGCTTCGGCGTCCCCTTCATGAAAACCCTCAAGCTCAAACGCCCCTCTGGCCGACCTCAGGGCAAACTCGACGTCACTGTCACCGTTCGAGAGACTCCAGGATCAAATTACGCGCTTCCTTATGGCGATCCATACGCGCCGGAAAAGGGGAGCAAGTTCGGTGGGATGGGGACTGGACTGGCGGTCGGGGCGGTGGCCGGAGTTTTGGGTGGGGTGGCATTAGCGGAGGGACTGGAGGCTGTGGAAGGCGATGAGGATGATGATGATGACGATGATGCTGCTGCTGCTGCTGCTGCTGATGATGATGATGATGATGATGATGATGATGATGATGATGACGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

219

Amino Acids

23.36

Weight (kDa)

4.18

Isoelectric Point (pI)

27.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 23 - 117 4.8e-11 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 392
AatII GACGTC 2 cut(s) 315, 390
AccI GTMKAC 1 cut(s) 222
AccII CGCG 2 cut(s) 428, 452
AciI CCGC 4 cut(s) 279, 497, 506, 539
AclWI GGATC 4 cut(s) 104, 113, 424, 437
AcoI YGGCCR 4 cut(s) 13, 198, 364, 510
AcyI GRCGYC 3 cut(s) 312, 324, 387
AfaI GTAC 2 cut(s) 160, 237
AfiI CCNNNNNNNGG 4 cut(s) 102, 272, 373, 461
AgsI TTSAA 1 cut(s) 121
AhdI GACNNNNNGTC 1 cut(s) 497
AjnI CCWGG 1 cut(s) 412
AluBI AGCT 1 cut(s) 349
AluI AGCT 1 cut(s) 349
Alw26I GTCTC 2 cut(s) 167, 401
AlwI GGATC 4 cut(s) 104, 113, 424, 437
AoxI GGCC 6 cut(s) 13, 76, 198, 244, 364, 510
ApeKI GCWGC 6 cut(s) 593, 596, 599, 602, 605, 608
ArsI GACNNNNNNTTYG 4 cut(s) 302, 334, 372, 404
AspLEI GCGC 3 cut(s) 72, 430, 454
AspS9I GGNCC 1 cut(s) 77
AsuHPI GGTGA 2 cut(s) 40, 388
AxyI CCTNAGG 1 cut(s) 372
BbsI GAAGAC 1 cut(s) 150
BbvI GCAGC 6 cut(s) 580, 583, 586, 589, 592, 595
BccI CCATC 3 cut(s) 41, 291, 475
BceAI ACGGC 5 cut(s) 28, 80, 91, 163, 231
BcgI CGANNNNNNTGC 2 cut(s) 575, 609
BciT130I CCWGG 1 cut(s) 414
BcoDI GTCTC 2 cut(s) 167, 401
BglI GCCNNNNNGGC 1 cut(s) 363
BisI GCNGC 6 cut(s) 594, 597, 600, 603, 606, 609
BlsI GCNGC 6 cut(s) 595, 598, 601, 604, 607, 610
Bme1390I CCNGG 1 cut(s) 414
BmeRI GACNNNNNGTC 1 cut(s) 497
BmgT120I GGNCC 1 cut(s) 77
BmiI GGNNCC 1 cut(s) 277
BmrFI CCNGG 1 cut(s) 414
BmsI GCATC 1 cut(s) 580
BoxI GACNNNNGTC 1 cut(s) 179
BpiI GAAGAC 1 cut(s) 150
BpmI CTGGAG 2 cut(s) 396, 569
BpuEI CTTGAG 1 cut(s) 329
Bsa29I ATCGAT 1 cut(s) 108
BsaHI GRCGYC 3 cut(s) 312, 324, 387
BsaJI CCNNGG 1 cut(s) 10
Bsc4I CCNNNNNNNGG 4 cut(s) 102, 272, 373, 461
Bse1I ACTGG 4 cut(s) 71, 493, 498, 552
Bse21I CCTNAGG 1 cut(s) 372
BseBI CCWGG 1 cut(s) 414
BseCI ATCGAT 1 cut(s) 108
BseDI CCNNGG 1 cut(s) 10
BseGI GGATG 3 cut(s) 5, 486, 577
BseLI CCNNNNNNNGG 4 cut(s) 102, 272, 373, 461
BseMII CTCAG 2 cut(s) 301, 386
BseNI ACTGG 4 cut(s) 71, 493, 498, 552
BseX3I CGGCCG 1 cut(s) 13
BseXI GCAGC 6 cut(s) 580, 583, 586, 589, 592, 595
Bsh1236I CGCG 2 cut(s) 428, 452
Bsh1285I CGRYCG 2 cut(s) 16, 501
BshFI GGCC 6 cut(s) 15, 78, 200, 246, 366, 512
BshVI ATCGAT 1 cut(s) 108
BsiEI CGRYCG 2 cut(s) 16, 501
BsiSI CCGG 3 cut(s) 197, 455, 513
BslFI GGGAC 3 cut(s) 311, 499, 558
BslI CCNNNNNNNGG 4 cut(s) 102, 272, 373, 461
BsmAI GTCTC 2 cut(s) 167, 401
BsmFI GGGAC 3 cut(s) 311, 499, 558
BsnI GGCC 6 cut(s) 15, 78, 200, 246, 366, 512
Bsp1407I TGTACA 1 cut(s) 158
Bsp143I GATC 4 cut(s) 105, 109, 416, 442
BspACI CCGC 4 cut(s) 279, 497, 506, 539
BspANI GGCC 6 cut(s) 15, 78, 200, 246, 366, 512
BspCNI CTCAG 2 cut(s) 300, 385
BspDI ATCGAT 1 cut(s) 108
BspFNI CGCG 2 cut(s) 428, 452
BspHI TCATGA 1 cut(s) 333
BspLI GGNNCC 1 cut(s) 277
BspPI GGATC 4 cut(s) 104, 113, 424, 437
BsrGI TGTACA 1 cut(s) 158
BsrI ACTGG 4 cut(s) 71, 493, 498, 552
BssECI CCNNGG 1 cut(s) 10
BssMI GATC 4 cut(s) 105, 109, 416, 442
BssNAI GTATAC 1 cut(s) 223
BssNI GRCGYC 3 cut(s) 312, 324, 387
Bst1107I GTATAC 1 cut(s) 223
Bst2UI CCWGG 1 cut(s) 414
Bst4CI ACNGT 2 cut(s) 394, 400
Bst6I CTCTTC 1 cut(s) 246
BstACI GRCGYC 3 cut(s) 312, 324, 387
BstAUI TGTACA 1 cut(s) 158
BstC8I GCNNGC 1 cut(s) 244
BstDEI CTNAG 2 cut(s) 287, 372
BstDSI CCRYGG 1 cut(s) 10
BstEII GGTNACC 1 cut(s) 28
BstF5I GGATG 3 cut(s) 5, 486, 577
BstFNI CGCG 2 cut(s) 428, 452
BstHHI GCGC 3 cut(s) 72, 430, 454
BstKTI GATC 4 cut(s) 108, 112, 419, 445
BstMAI GTCTC 2 cut(s) 167, 401
BstMBI GATC 4 cut(s) 105, 109, 416, 442
BstMCI CGRYCG 2 cut(s) 16, 501
BstMWI GCNNNNNNNGC 6 cut(s) 363, 503, 599, 602, 605, 608
BstNI CCWGG 1 cut(s) 414
BstPAI GACNNNNGTC 1 cut(s) 179
BstPI GGTNACC 1 cut(s) 28
BstSCI CCNGG 1 cut(s) 412
BstUI CGCG 2 cut(s) 428, 452
BstV1I GCAGC 6 cut(s) 580, 583, 586, 589, 592, 595
BstV2I GAAGAC 1 cut(s) 150
BstZ17I GTATAC 1 cut(s) 223
BstZI CGGCCG 1 cut(s) 13
Bsu15I ATCGAT 1 cut(s) 108
Bsu36I CCTNAGG 1 cut(s) 372
BsuRI GGCC 6 cut(s) 15, 78, 200, 246, 366, 512
BsuTUI ATCGAT 1 cut(s) 108
BtgI CCRYGG 1 cut(s) 10
BtgZI GCGATG 2 cut(s) 169, 579
BtsCI GGATG 3 cut(s) 5, 486, 577
BtsIMutI CAGTG 1 cut(s) 390
Cac8I GCNNGC 1 cut(s) 244
CciI TCATGA 1 cut(s) 333
CfoI GCGC 3 cut(s) 72, 430, 454
Cfr13I GGNCC 1 cut(s) 77
ClaI ATCGAT 1 cut(s) 108
CseI GACGC 1 cut(s) 313
Csp6I GTAC 2 cut(s) 159, 236
CspCI CAANNNNNGTGG 4 cut(s) 118, 153, 457, 492
CviAII CATG 1 cut(s) 334
CviQI GTAC 2 cut(s) 159, 236
DdeI CTNAG 2 cut(s) 287, 372
DpnI GATC 4 cut(s) 107, 111, 418, 444
DpnII GATC 4 cut(s) 105, 109, 416, 442
DrdI GACNNNNNNGTC 1 cut(s) 392
DriI GACNNNNNGTC 1 cut(s) 497
DseDI GACNNNNNNGTC 1 cut(s) 392
EaeI YGGCCR 4 cut(s) 13, 198, 364, 510
EagI CGGCCG 1 cut(s) 13
Eam1104I CTCTTC 1 cut(s) 246
Eam1105I GACNNNNNGTC 1 cut(s) 497
EarI CTCTTC 1 cut(s) 246
EciI GGCGGA 1 cut(s) 268
EclXI CGGCCG 1 cut(s) 13
Eco32I GATATC 1 cut(s) 52
Eco52I CGGCCG 1 cut(s) 13
Eco81I CCTNAGG 1 cut(s) 372
Eco91I GGTNACC 1 cut(s) 28
EcoO65I GGTNACC 1 cut(s) 28
EcoRII CCWGG 1 cut(s) 412
EcoRV GATATC 1 cut(s) 52
FaeI CATG 1 cut(s) 337
FaiI YATR 6 cut(s) 91, 103, 223, 335, 438, 448
FaqI GGGAC 3 cut(s) 311, 499, 558
FatI CATG 1 cut(s) 333
FblI GTMKAC 1 cut(s) 222
Fnu4HI GCNGC 6 cut(s) 594, 597, 600, 603, 606, 609
FokI GGATG 2 cut(s) 493, 584
Fsp4HI GCNGC 6 cut(s) 594, 597, 600, 603, 606, 609
GlaI GCGC 3 cut(s) 71, 429, 453
GluI GCNGC 6 cut(s) 594, 597, 600, 603, 606, 609
GsuI CTGGAG 2 cut(s) 396, 569
HaeIII GGCC 6 cut(s) 15, 78, 200, 246, 366, 512
HapII CCGG 3 cut(s) 197, 455, 513
HgaI GACGC 1 cut(s) 313
HhaI GCGC 3 cut(s) 72, 430, 454
Hin1I GRCGYC 3 cut(s) 312, 324, 387
Hin1II CATG 1 cut(s) 337
Hin6I GCGC 3 cut(s) 70, 428, 452
HinP1I GCGC 3 cut(s) 70, 428, 452
HincII GTYRAC 1 cut(s) 139
HindII GTYRAC 1 cut(s) 139
HinfI GANTC 1 cut(s) 409
HpaII CCGG 3 cut(s) 197, 455, 513
HphI GGTGA 2 cut(s) 40, 388
Hpy166II GTNNAC 4 cut(s) 64, 139, 223, 238
Hpy188I TCNGA 1 cut(s) 114
Hpy188III TCNNGA 3 cut(s) 55, 334, 404
Hpy8I GTNNAC 4 cut(s) 64, 139, 223, 238
Hpy99I CGWCG 3 cut(s) 209, 317, 389
HpyAV CCTTC 2 cut(s) 340, 554
HpyCH4III ACNGT 2 cut(s) 394, 400
HpyCH4IV ACGT 4 cut(s) 60, 164, 312, 387
HpyF10VI GCNNNNNNNGC 6 cut(s) 363, 503, 599, 602, 605, 608
HpyF3I CTNAG 2 cut(s) 287, 372
HpySE526I ACGT 4 cut(s) 60, 164, 312, 387
Hsp92I GRCGYC 3 cut(s) 312, 324, 387
Hsp92II CATG 1 cut(s) 337
HspAI GCGC 3 cut(s) 70, 428, 452
Kzo9I GATC 4 cut(s) 105, 109, 416, 442
LmnI GCTCC 2 cut(s) 281, 465
Lsp1109I GCAGC 6 cut(s) 580, 583, 586, 589, 592, 595
LweI GCATC 1 cut(s) 580
MaeII ACGT 4 cut(s) 60, 164, 312, 387
MaeIII GTNAC 3 cut(s) 28, 388, 394
MalI GATC 4 cut(s) 107, 111, 418, 444
MboI GATC 4 cut(s) 105, 109, 416, 442
MboII GAAGA 2 cut(s) 155, 263
MluCI AATT 1 cut(s) 421
MlyI GAGTC 1 cut(s) 403
MspI CCGG 3 cut(s) 197, 455, 513
MspR9I CCNGG 1 cut(s) 414
MvaI CCWGG 1 cut(s) 414
MvnI CGCG 2 cut(s) 428, 452
MwoI GCNNNNNNNGC 6 cut(s) 363, 503, 599, 602, 605, 608
NdeII GATC 4 cut(s) 105, 109, 416, 442
NlaIII CATG 1 cut(s) 337
NlaIV GGNNCC 1 cut(s) 277
NmeAIII GCCGAG 1 cut(s) 41
NmuCI GTSAC 3 cut(s) 28, 388, 394
PagI TCATGA 1 cut(s) 333
PcsI WCGNNNNNNNCGW 2 cut(s) 210, 321
PfoI TCCNGGA 1 cut(s) 412
PkrI GCNGC 6 cut(s) 595, 598, 601, 604, 607, 610
PleI GAGTC 1 cut(s) 403
PpsI GAGTC 1 cut(s) 403
PshAI GACNNNNGTC 1 cut(s) 179
Psp6I CCWGG 1 cut(s) 412
PspEI GGTNACC 1 cut(s) 28
PspGI CCWGG 1 cut(s) 412
PspN4I GGNNCC 1 cut(s) 277
PspPI GGNCC 1 cut(s) 77
RsaI GTAC 2 cut(s) 160, 237
RsaNI GTAC 2 cut(s) 159, 236
SatI GCNGC 6 cut(s) 594, 597, 600, 603, 606, 609
Sau3AI GATC 4 cut(s) 105, 109, 416, 442
Sau96I GGNCC 1 cut(s) 77
SchI GAGTC 1 cut(s) 403
ScrFI CCNGG 1 cut(s) 414
SetI ASST 9 cut(s) 30, 63, 167, 196, 222, 315, 351, 373, 390
SfaNI GCATC 1 cut(s) 580
SmlI CTYRAG 1 cut(s) 344
SmoI CTYRAG 1 cut(s) 344
Sse9I AATT 1 cut(s) 421
SsiI CCGC 4 cut(s) 279, 497, 506, 539
StyD4I CCNGG 1 cut(s) 412
TaaI ACNGT 2 cut(s) 394, 400
TaiI ACGT 4 cut(s) 63, 167, 315, 390
TaqI TCGA 3 cut(s) 108, 384, 403
TasI AATT 1 cut(s) 421
TatI WGTACW 1 cut(s) 158
TscAI CASTG 1 cut(s) 397
TseFI GTSAC 3 cut(s) 28, 388, 394
TseI GCWGC 6 cut(s) 593, 596, 599, 602, 605, 608
Tsp45I GTSAC 3 cut(s) 28, 388, 394
TspDTI ATGAA 2 cut(s) 322, 350
TspGWI ACGGA 1 cut(s) 284
TspRI CASTG 1 cut(s) 397
XmiI GTMKAC 1 cut(s) 222
ZraI GACGTC 2 cut(s) 313, 388
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.