FvH4_6g31310

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0004046
Physical Location & Seq
Reverse (-)
24475854 .. 24477433
1580 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g31310.t1

Sequence Viewer

Length: 834 bp
ATGGCGTCCACCCACGAAGTCCAAGTGAAGATCTCCTCCGCCAAAGACCTCAAGAACGTCAACTGGAGACACGGCCCCGTCAAGCCCTACGCCGTCGCGTGGGTCGAGCCGAGCAACAAATGCTCCACCAAGGTCGACGAGCACGGCGACACCTCACCCGAATGGGACGAGACCTTGATCATCCCTATCCCCGGTCCTGTCAACAACGACACCACTCTCTACATCGACATCGTGCATGCCGGAACCGAGCCTGACACCAAGCAGCTGGTGGGATCGGCTAGGCTCCCGCTGCGCGACGTTGTCGATGACGTCGGGTACGACGAGCGCTCTAGTCGCACTCTGACCATCAAACGGCCCTCCGGTAGGCCTCAGGGTAAGGTGGACGTCAAGGTTACGGTGAGGGAGCCGCGTTACCGTGCGCCGGAGCCGTACTATGGACCGCCTTATGGTGTCCCACCTGCAGGCTCTGGAGGTTACCGTGCTCCACCGCCGCCGCATGCGTATGGCGATCCGTACGCTGCACCACCGCCGCCGCCGCCGGTGTATGGTGGGATGTACTCATCACAGGCTTCAAGGTATCCCTACAATGCGGCTGCGGCAGCGTACGGTCAGCCAGCTCAGGCGAGTTATGGTCAAGTGCAGTACGGGCAGGAGGAGAAGAAGAAGAGCGAGTTTGGTGGGATGGGAACAGGATTGGCTGTGGGGGCGGTAGCGGGGGCACTGGGTGCGTTTGCGTTGGCGGAGGGAGTTGATTATGTGGAGGATAAAATTGCAGATGACGTGGCGGAGAAGGTGGTGGAGGATCTGAGCTACTATGGCGACGATGACTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

278

Amino Acids

29.77

Weight (kDa)

5.02

Isoelectric Point (pI)

34.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 6 - 105 6.5e-12 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 466
AatII GACGTC 2 cut(s) 312, 387
Acc36I ACCTGC 1 cut(s) 466
AccI GTMKAC 1 cut(s) 135
AccII CGCG 3 cut(s) 98, 294, 409
AclWI GGATC 3 cut(s) 280, 503, 810
AcyI GRCGYC 3 cut(s) 5, 309, 384
AdeI CACNNNGTG 1 cut(s) 725
AfaI GTAC 6 cut(s) 317, 431, 515, 557, 605, 644
AfeI AGCGCT 1 cut(s) 326
AfiI CCNNNNNNNGG 9 cut(s) 99, 191, 351, 363, 421, 434, 446, 461, 545
AgsI TTSAA 1 cut(s) 573
AjiI CACGTC 1 cut(s) 781
AluBI AGCT 3 cut(s) 265, 617, 810
AluI AGCT 3 cut(s) 265, 617, 810
Alw21I GWGCWC 2 cut(s) 144, 484
Alw26I GTCTC 2 cut(s) 61, 164
AlwI GGATC 3 cut(s) 280, 503, 810
AlwNI CAGNNNCTG 1 cut(s) 467
Aor51HI AGCGCT 1 cut(s) 326
AoxI GGCC 3 cut(s) 73, 353, 365
ApeKI GCWGC 5 cut(s) 262, 289, 518, 593, 599
AspLEI GCGC 3 cut(s) 294, 327, 421
AspS9I GGNCC 4 cut(s) 74, 194, 354, 437
AsuC2I CCSGG 1 cut(s) 192
AsuHPI GGTGA 2 cut(s) 147, 409
AvaII GGWCC 2 cut(s) 194, 437
AxyI CCTNAGG 1 cut(s) 369
BaeGI GKGCMC 1 cut(s) 721
Bbv12I GWGCWC 2 cut(s) 144, 484
BbvI GCAGC 5 cut(s) 274, 276, 505, 580, 611
BccI CCATC 2 cut(s) 353, 676
BceAI ACGGC 5 cut(s) 77, 88, 160, 368, 412
BciVI GTATCC 1 cut(s) 588
BclI TGATCA 1 cut(s) 177
BcnI CCSGG 1 cut(s) 192
BcoDI GTCTC 2 cut(s) 61, 164
BfaI CTAG 2 cut(s) 279, 330
BfmI CTRYAG 1 cut(s) 459
BfoI RGCGCY 1 cut(s) 328
BfuAI ACCTGC 1 cut(s) 466
BfuI GTATCC 1 cut(s) 588
BglII AGATCT 1 cut(s) 30
Bme1390I CCNGG 1 cut(s) 192
Bme18I GGWCC 2 cut(s) 194, 437
BmgBI CACGTC 1 cut(s) 781
BmgT120I GGNCC 4 cut(s) 74, 194, 354, 437
BmiI GGNNCC 5 cut(s) 76, 244, 284, 405, 426
BmrFI CCNGG 1 cut(s) 192
BmrI ACTGGG 1 cut(s) 731
BmuI ACTGGG 1 cut(s) 731
BpmI CTGGAG 2 cut(s) 85, 489
Bpu10I CCTNAGC 1 cut(s) 618
BpuEI CTTGAG 1 cut(s) 35
BpuMI CCSGG 1 cut(s) 192
BsaHI GRCGYC 3 cut(s) 5, 309, 384
BsaI GGTCTC 1 cut(s) 164
BsaJI CCNNGG 2 cut(s) 129, 190
BsaWI WCCGGW 1 cut(s) 359
BsaXI ACNNNNNCTCC 2 cut(s) 107, 137
Bsc4I CCNNNNNNNGG 9 cut(s) 99, 191, 351, 363, 421, 434, 446, 461, 545
Bse118I RCCGGY 1 cut(s) 538
Bse1I ACTGG 2 cut(s) 68, 726
Bse21I CCTNAGG 1 cut(s) 369
BseDI CCNNGG 2 cut(s) 129, 190
BseGI GGATG 3 cut(s) 180, 558, 687
BseLI CCNNNNNNNGG 9 cut(s) 99, 191, 351, 363, 421, 434, 446, 461, 545
BseMII CTCAG 3 cut(s) 383, 632, 797
BseNI ACTGG 2 cut(s) 68, 726
BseRI GAGGAG 2 cut(s) 25, 668
BseSI GKGCMC 1 cut(s) 721
BseXI GCAGC 5 cut(s) 274, 276, 505, 580, 611
BsgI GTGCAG 2 cut(s) 504, 659
Bsh1236I CGCG 3 cut(s) 98, 294, 409
BshFI GGCC 3 cut(s) 75, 355, 367
BsiHKAI GWGCWC 2 cut(s) 144, 484
BsiSI CCGG 5 cut(s) 192, 240, 360, 422, 539
BsiWI CGTACG 2 cut(s) 513, 603
BslFI GGGAC 2 cut(s) 179, 437
BslI CCNNNNNNNGG 9 cut(s) 99, 191, 351, 363, 421, 434, 446, 461, 545
BsmAI GTCTC 2 cut(s) 61, 164
BsmFI GGGAC 2 cut(s) 179, 437
BsnI GGCC 3 cut(s) 75, 355, 367
Bso31I GGTCTC 1 cut(s) 164
Bsp1286I GDGCHC 3 cut(s) 144, 484, 721
Bsp143I GATC 5 cut(s) 30, 177, 272, 508, 802
BspANI GGCC 3 cut(s) 75, 355, 367
BspCNI CTCAG 3 cut(s) 382, 631, 798
BspFNI CGCG 3 cut(s) 98, 294, 409
BspLI GGNNCC 5 cut(s) 76, 244, 284, 405, 426
BspMAI CTGCAG 1 cut(s) 463
BspMI ACCTGC 1 cut(s) 466
BspPI GGATC 3 cut(s) 280, 503, 810
BspQI GCTCTTC 1 cut(s) 659
BspTNI GGTCTC 1 cut(s) 164
BsrFI RCCGGY 1 cut(s) 538
BsrI ACTGG 2 cut(s) 68, 726
BssAI RCCGGY 1 cut(s) 538
BssECI CCNNGG 2 cut(s) 129, 190
BssMI GATC 5 cut(s) 30, 177, 272, 508, 802
BssNI GRCGYC 3 cut(s) 5, 309, 384
BssT1I CCWWGG 1 cut(s) 129
Bst4CI ACNGT 4 cut(s) 397, 416, 479, 608
Bst6I CTCTTC 1 cut(s) 659
BstACI GRCGYC 3 cut(s) 5, 309, 384
BstAPI GCANNNNNTGC 2 cut(s) 120, 725
BstC8I GCNNGC 4 cut(s) 237, 463, 498, 615
BstDEI CTNAG 3 cut(s) 369, 618, 806
BstEII GGTNACC 1 cut(s) 473
BstENI CCTNNNNNAGG 1 cut(s) 361
BstF5I GGATG 3 cut(s) 180, 558, 687
BstFNI CGCG 3 cut(s) 98, 294, 409
BstH2I RGCGCY 1 cut(s) 328
BstHHI GCGC 3 cut(s) 294, 327, 421
BstKTI GATC 5 cut(s) 33, 180, 275, 511, 805
BstMAI GTCTC 2 cut(s) 61, 164
BstMBI GATC 5 cut(s) 30, 177, 272, 508, 802
BstNSI RCATGY 2 cut(s) 239, 500
BstPI GGTNACC 1 cut(s) 473
BstSCI CCNGG 1 cut(s) 190
BstSFI CTRYAG 1 cut(s) 459
BstSLI GKGCMC 1 cut(s) 721
BstUI CGCG 3 cut(s) 98, 294, 409
BstV1I GCAGC 5 cut(s) 274, 276, 505, 580, 611
BstX2I RGATCY 2 cut(s) 30, 802
BstXI CCANNNNNNTGG 1 cut(s) 265
BstYI RGATCY 2 cut(s) 30, 802
Bsu36I CCTNAGG 1 cut(s) 369
BsuI GTATCC 1 cut(s) 588
BsuRI GGCC 3 cut(s) 75, 355, 367
BtrI CACGTC 1 cut(s) 781
BtsCI GGATG 3 cut(s) 180, 558, 687
BtsIMutI CAGTG 1 cut(s) 719
BveI ACCTGC 1 cut(s) 466
Cac8I GCNNGC 4 cut(s) 237, 463, 498, 615
CaiI CAGNNNCTG 1 cut(s) 467
CfoI GCGC 3 cut(s) 294, 327, 421
Cfr10I RCCGGY 1 cut(s) 538
Cfr13I GGNCC 4 cut(s) 74, 194, 354, 437
Csp6I GTAC 6 cut(s) 316, 430, 514, 556, 604, 643
CviAII CATG 2 cut(s) 236, 497
CviQI GTAC 6 cut(s) 316, 430, 514, 556, 604, 643
DdeI CTNAG 3 cut(s) 369, 618, 806
DpnI GATC 5 cut(s) 32, 179, 274, 510, 804
DpnII GATC 5 cut(s) 30, 177, 272, 508, 802
DraIII CACNNNGTG 1 cut(s) 725
Eam1104I CTCTTC 1 cut(s) 659
EarI CTCTTC 1 cut(s) 659
EciI GGCGGA 3 cut(s) 28, 755, 800
Eco130I CCWWGG 1 cut(s) 129
Eco147I AGGCCT 1 cut(s) 367
Eco31I GGTCTC 1 cut(s) 164
Eco47I GGWCC 2 cut(s) 194, 437
Eco47III AGCGCT 1 cut(s) 326
Eco81I CCTNAGG 1 cut(s) 369
Eco91I GGTNACC 1 cut(s) 473
EcoNI CCTNNNNNAGG 1 cut(s) 361
EcoO65I GGTNACC 1 cut(s) 473
EcoT14I CCWWGG 1 cut(s) 129
ErhI CCWWGG 1 cut(s) 129
FaeI CATG 2 cut(s) 239, 500
FaiI YATR 9 cut(s) 237, 435, 447, 498, 504, 546, 630, 756, 816
FaqI GGGAC 2 cut(s) 179, 437
FatI CATG 2 cut(s) 235, 496
FauI CCCGC 2 cut(s) 294, 706
FbaI TGATCA 1 cut(s) 177
FblI GTMKAC 1 cut(s) 135
FokI GGATG 3 cut(s) 167, 565, 694
FspBI CTAG 2 cut(s) 279, 330
GlaI GCGC 3 cut(s) 293, 326, 420
GsuI CTGGAG 2 cut(s) 85, 489
HaeII RGCGCY 1 cut(s) 328
HaeIII GGCC 3 cut(s) 75, 355, 367
HapII CCGG 5 cut(s) 192, 240, 360, 422, 539
HhaI GCGC 3 cut(s) 294, 327, 421
Hin1I GRCGYC 3 cut(s) 5, 309, 384
Hin1II CATG 2 cut(s) 239, 500
Hin6I GCGC 3 cut(s) 292, 325, 419
HinP1I GCGC 3 cut(s) 292, 325, 419
HincII GTYRAC 3 cut(s) 61, 136, 202
HindII GTYRAC 3 cut(s) 61, 136, 202
HpaII CCGG 5 cut(s) 192, 240, 360, 422, 539
HphI GGTGA 2 cut(s) 147, 409
Hpy166II GTNNAC 5 cut(s) 9, 61, 136, 202, 382
Hpy188I TCNGA 2 cut(s) 342, 807
Hpy188III TCNNGA 2 cut(s) 52, 468
Hpy8I GTNNAC 5 cut(s) 9, 61, 136, 202, 382
Hpy99I CGWCG 6 cut(s) 98, 140, 299, 314, 323, 824
HpyAV CCTTC 1 cut(s) 784
HpyCH4III ACNGT 4 cut(s) 397, 416, 479, 608
HpyCH4IV ACGT 5 cut(s) 57, 297, 309, 384, 780
HpyCH4V TGCA 5 cut(s) 235, 461, 521, 640, 773
HpyF3I CTNAG 3 cut(s) 369, 618, 806
HpySE526I ACGT 5 cut(s) 57, 297, 309, 384, 780
Hsp92I GRCGYC 3 cut(s) 5, 309, 384
Hsp92II CATG 2 cut(s) 239, 500
HspAI GCGC 3 cut(s) 292, 325, 419
Ksp22I TGATCA 1 cut(s) 177
Kzo9I GATC 5 cut(s) 30, 177, 272, 508, 802
LguI GCTCTTC 1 cut(s) 659
LmnI GCTCC 5 cut(s) 128, 288, 403, 424, 487
Lsp1109I GCAGC 5 cut(s) 274, 276, 505, 580, 611
MaeI CTAG 2 cut(s) 279, 330
MaeII ACGT 5 cut(s) 57, 297, 309, 384, 780
MaeIII GTNAC 3 cut(s) 391, 410, 473
MalI GATC 5 cut(s) 32, 179, 274, 510, 804
MboI GATC 5 cut(s) 30, 177, 272, 508, 802
MboII GAAGA 4 cut(s) 40, 670, 673, 676
MflI RGATCY 2 cut(s) 30, 802
MhlI GDGCHC 3 cut(s) 144, 484, 721
MluCI AATT 1 cut(s) 768
MslI CAYNNNNRTG 2 cut(s) 160, 501
MspA1I CMGCKG 2 cut(s) 265, 289
MspI CCGG 5 cut(s) 192, 240, 360, 422, 539
MspR9I CCNGG 1 cut(s) 192
MvnI CGCG 3 cut(s) 98, 294, 409
NciI CCSGG 1 cut(s) 192
NdeII GATC 5 cut(s) 30, 177, 272, 508, 802
NlaIII CATG 2 cut(s) 239, 500
NlaIV GGNNCC 5 cut(s) 76, 244, 284, 405, 426
NmeAIII GCCGAG 1 cut(s) 135
NspI RCATGY 2 cut(s) 239, 500
PaeI GCATGC 2 cut(s) 239, 500
PaqCI CACCTGC 1 cut(s) 466
PceI AGGCCT 1 cut(s) 367
PciSI GCTCTTC 1 cut(s) 659
PcsI WCGNNNNNNNCGW 4 cut(s) 102, 144, 315, 318
Pfl23II CGTACG 2 cut(s) 513, 603
PflFI GACNNNGTC 1 cut(s) 299
PspEI GGTNACC 1 cut(s) 473
PspLI CGTACG 2 cut(s) 513, 603
PspN4I GGNNCC 5 cut(s) 76, 244, 284, 405, 426
PspPI GGNCC 4 cut(s) 74, 194, 354, 437
PstI CTGCAG 1 cut(s) 463
PstNI CAGNNNCTG 1 cut(s) 467
PsuI RGATCY 2 cut(s) 30, 802
PsyI GACNNNGTC 1 cut(s) 299
PvuII CAGCTG 1 cut(s) 265
RsaI GTAC 6 cut(s) 317, 431, 515, 557, 605, 644
RsaNI GTAC 6 cut(s) 316, 430, 514, 556, 604, 643
RseI CAYNNNNRTG 2 cut(s) 160, 501
SalI GTCGAC 1 cut(s) 134
SapI GCTCTTC 1 cut(s) 659
Sau3AI GATC 5 cut(s) 30, 177, 272, 508, 802
Sau96I GGNCC 4 cut(s) 74, 194, 354, 437
SbfI CCTGCAGG 1 cut(s) 463
ScrFI CCNGG 1 cut(s) 192
SdaI CCTGCAGG 1 cut(s) 463
SduI GDGCHC 3 cut(s) 144, 484, 721
SfcI CTRYAG 1 cut(s) 459
SgrAI CRCCGGYG 1 cut(s) 538
SinI GGWCC 2 cut(s) 194, 437
SmiMI CAYNNNNRTG 2 cut(s) 160, 501
SmlI CTYRAG 1 cut(s) 50
SmoI CTYRAG 1 cut(s) 50
SphI GCATGC 2 cut(s) 239, 500
Sse8387I CCTGCAGG 1 cut(s) 463
Sse9I AATT 1 cut(s) 768
SseBI AGGCCT 1 cut(s) 367
SspMI CTAG 2 cut(s) 279, 330
StuI AGGCCT 1 cut(s) 367
StyD4I CCNGG 1 cut(s) 190
StyI CCWWGG 1 cut(s) 129
TaaI ACNGT 4 cut(s) 397, 416, 479, 608
TaiI ACGT 5 cut(s) 60, 300, 312, 387, 783
TaqI TCGA 4 cut(s) 105, 135, 225, 303
TasI AATT 1 cut(s) 768
TatI WGTACW 1 cut(s) 555
TauI GCSGC 8 cut(s) 409, 493, 496, 532, 535, 538, 593, 599
TscAI CASTG 1 cut(s) 726
TseI GCWGC 5 cut(s) 262, 289, 518, 593, 599
TspGWI ACGGA 1 cut(s) 501
TspRI CASTG 1 cut(s) 726
Tth111I GACNNNGTC 1 cut(s) 299
VpaK11BI GGWCC 2 cut(s) 194, 437
XagI CCTNNNNNAGG 1 cut(s) 361
XceI RCATGY 2 cut(s) 239, 500
XcmI CCANNNNNNNNNTGG 1 cut(s) 265
XmiI GTMKAC 1 cut(s) 135
XspI CTAG 2 cut(s) 279, 330
ZraI GACGTC 2 cut(s) 310, 385
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.