Rh6BG033300

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0004046
Physical Location & Seq
Forward (+)
4975213 .. 4976519
1307 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG033300.1

Sequence Viewer

Length: 822 bp
ATGGCTTCCGGCTACGAAGTAGAGGTCACAATTTCTTCCGCCAAAGACCTCAAAAACGTGAATTGGCGCCACGGAGAGCTGAAGCCCTACGCTGTTGTTTGGGTGGATCCAAACAATAAATGCTCCACCCATGTGGACGAATACGGTGACGACTCGCCCACGTGGGATGAGACTCTCTCGATCCCGTTACGGGGTCCCGTCGAGGACTCGACCCTTTTCGTGGACATCATCCACGTCAAGGCGGACGAGGACGTCAAGCCGTTGATCGGATCCGCAAAACTCCCGCTTAGGGAGGTCCTCGATGACGTGGGGCTTGGGGCACCGTCCGTTCACAAGTTGAAGCTCAAGCGCCCCTCGGGTAGGCCCCACGGGAAGTTGGAAGTCAAAGTCACAGTTCGCCCGCCCCGTTACCAAGCACCTGACCCGTATCACGCCCCCGCGCCCTACGGGTACGGGATCGCACCGTCGCAGTCGAGAGACTACGGCGCACCTCAGCAGTATAATTACCAATATGCCCCTCCTGCCCAACCCCAATATTACCAAAGCAGCAGCGTCCCACAAGCTGCAGGGTACGGTTACAATCAGCCATCTTACGATCAGAGTCAGTATGGGAGTGGCGGGTCGTACGGGTACGGGTCGTCGGAGAAGCCGAAGGAGAAGAGCAAGTTTGGGGTCGGGACCGGATTGGCTGTGGGAGCCGTAGCCGGAGTGTTGGGTGGACTTGCGATTGCTGAAGGAGTGGATTACGTGGAGGACAAGATTGCTGATGATGCAGCGGAGAAAAATAATAAGATCTTCTTCCCACTCCATCCAAGTACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

273

Amino Acids

29.59

Weight (kDa)

5.65

Isoelectric Point (pI)

36.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 7 - 100 1.5e-10 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 251
AatII GACGTC 1 cut(s) 255
AccB1I GGYRCC 2 cut(s) 66, 319
AccII CGCG 1 cut(s) 440
AciI CCGC 8 cut(s) 39, 242, 273, 284, 401, 438, 618, 776
AclWI GGATC 6 cut(s) 101, 114, 175, 264, 277, 464
AcuI CTGAAG 2 cut(s) 101, 753
AcvI CACGTG 1 cut(s) 162
AcyI GRCGYC 2 cut(s) 67, 252
AfaI GTAC 5 cut(s) 452, 572, 626, 632, 817
AfiI CCNNNNNNNGG 7 cut(s) 190, 191, 220, 238, 266, 289, 360
AgsI TTSAA 1 cut(s) 340
AjiI CACGTC 2 cut(s) 235, 307
AleI CACNNNNGTG 1 cut(s) 131
AluBI AGCT 3 cut(s) 79, 343, 563
AluI AGCT 3 cut(s) 79, 343, 563
Alw26I GTCTC 2 cut(s) 164, 471
AlwI GGATC 6 cut(s) 101, 114, 175, 264, 277, 464
Ama87I CYCGRG 1 cut(s) 355
AoxI GGCC 1 cut(s) 362
ApeKI GCWGC 4 cut(s) 546, 549, 563, 773
ArsI GACNNNNNNTTYG 2 cut(s) 9, 41
AspLEI GCGC 4 cut(s) 69, 351, 442, 488
AspS9I GGNCC 4 cut(s) 194, 295, 363, 678
AsuHPI GGTGA 1 cut(s) 158
AvaI CYCGRG 1 cut(s) 355
AvaII GGWCC 3 cut(s) 194, 295, 678
BaeGI GKGCMC 1 cut(s) 322
BamHI GGATCC 2 cut(s) 106, 269
BanI GGYRCC 2 cut(s) 66, 319
BbrPI CACGTG 1 cut(s) 162
BbvCI CCTCAGC 1 cut(s) 492
BbvI GCAGC 4 cut(s) 550, 558, 561, 785
BccI CCATC 2 cut(s) 595, 816
BceAI ACGGC 3 cut(s) 244, 499, 683
BcoDI GTCTC 2 cut(s) 164, 471
BfmI CTRYAG 1 cut(s) 564
BfoI RGCGCY 2 cut(s) 70, 352
BglII AGATCT 1 cut(s) 792
BisI GCNGC 4 cut(s) 547, 550, 564, 774
BlsI GCNGC 4 cut(s) 548, 551, 565, 775
Bme18I GGWCC 3 cut(s) 194, 295, 678
BmeT110I CYCGRG 1 cut(s) 355
BmgBI CACGTC 2 cut(s) 235, 307
BmgT120I GGNCC 4 cut(s) 194, 295, 363, 678
BmiI GGNNCC 9 cut(s) 68, 108, 195, 196, 271, 321, 365, 679, 697
BmsI GCATC 1 cut(s) 760
BplI GAGNNNNNCTC 2 cut(s) 161, 193
Bpu10I CCTNAGC 2 cut(s) 287, 492
BpuEI CTTGAG 1 cut(s) 329
BsaAI YACGTR 2 cut(s) 162, 748
BsaHI GRCGYC 2 cut(s) 67, 252
BsaJI CCNNGG 3 cut(s) 70, 354, 367
BsaWI WCCGGW 1 cut(s) 680
Bsc4I CCNNNNNNNGG 7 cut(s) 190, 191, 220, 238, 266, 289, 360
BseDI CCNNGG 3 cut(s) 70, 354, 367
BseGI GGATG 3 cut(s) 172, 228, 808
BseLI CCNNNNNNNGG 7 cut(s) 190, 191, 220, 238, 266, 289, 360
BseMII CTCAG 1 cut(s) 506
BseSI GKGCMC 1 cut(s) 322
BseXI GCAGC 4 cut(s) 550, 558, 561, 785
Bsh1236I CGCG 1 cut(s) 440
BshFI GGCC 1 cut(s) 364
BshNI GGYRCC 2 cut(s) 66, 319
BsiHKCI CYCGRG 1 cut(s) 355
BsiSI CCGG 3 cut(s) 9, 681, 705
BsiWI CGTACG 1 cut(s) 624
BslFI GGGAC 3 cut(s) 180, 539, 691
BslI CCNNNNNNNGG 7 cut(s) 190, 191, 220, 238, 266, 289, 360
BsmAI GTCTC 2 cut(s) 164, 471
BsmFI GGGAC 3 cut(s) 180, 539, 691
BsnI GGCC 1 cut(s) 364
BsoBI CYCGRG 1 cut(s) 355
Bsp1286I GDGCHC 1 cut(s) 322
Bsp143I GATC 7 cut(s) 106, 180, 264, 269, 456, 595, 792
BspACI CCGC 8 cut(s) 39, 242, 273, 284, 401, 438, 618, 776
BspANI GGCC 1 cut(s) 364
BspCNI CTCAG 1 cut(s) 505
BspFNI CGCG 1 cut(s) 440
BspLI GGNNCC 9 cut(s) 68, 108, 195, 196, 271, 321, 365, 679, 697
BspMAI CTGCAG 1 cut(s) 568
BspPI GGATC 6 cut(s) 101, 114, 175, 264, 277, 464
BspQI GCTCTTC 1 cut(s) 653
BspT107I GGYRCC 2 cut(s) 66, 319
BssECI CCNNGG 3 cut(s) 70, 354, 367
BssMI GATC 7 cut(s) 106, 180, 264, 269, 456, 595, 792
BssNI GRCGYC 2 cut(s) 67, 252
Bst4CI ACNGT 5 cut(s) 146, 324, 394, 465, 575
Bst6I CTCTTC 1 cut(s) 653
BstACI GRCGYC 2 cut(s) 67, 252
BstBAI YACGTR 2 cut(s) 162, 748
BstC8I GCNNGC 1 cut(s) 401
BstDEI CTNAG 2 cut(s) 287, 492
BstDSI CCRYGG 2 cut(s) 70, 367
BstENI CCTNNNNNAGG 1 cut(s) 358
BstF5I GGATG 3 cut(s) 172, 228, 808
BstFNI CGCG 1 cut(s) 440
BstH2I RGCGCY 2 cut(s) 70, 352
BstHHI GCGC 4 cut(s) 69, 351, 442, 488
BstKTI GATC 7 cut(s) 109, 183, 267, 272, 459, 598, 795
BstMAI GTCTC 2 cut(s) 164, 471
BstMBI GATC 7 cut(s) 106, 180, 264, 269, 456, 595, 792
BstMWI GCNNNNNNNGC 3 cut(s) 521, 695, 770
BstSFI CTRYAG 1 cut(s) 564
BstSLI GKGCMC 1 cut(s) 322
BstUI CGCG 1 cut(s) 440
BstV1I GCAGC 4 cut(s) 550, 558, 561, 785
BstX2I RGATCY 3 cut(s) 106, 269, 792
BstXI CCANNNNNNTGG 1 cut(s) 133
BstYI RGATCY 3 cut(s) 106, 269, 792
BsuRI GGCC 1 cut(s) 364
BtgI CCRYGG 2 cut(s) 70, 367
BtrI CACGTC 2 cut(s) 235, 307
BtsCI GGATG 3 cut(s) 172, 228, 808
Cac8I GCNNGC 1 cut(s) 401
CfoI GCGC 4 cut(s) 69, 351, 442, 488
Cfr13I GGNCC 4 cut(s) 194, 295, 363, 678
CseI GACGC 1 cut(s) 541
Csp6I GTAC 5 cut(s) 451, 571, 625, 631, 816
CviAII CATG 1 cut(s) 131
CviQI GTAC 5 cut(s) 451, 571, 625, 631, 816
DdeI CTNAG 2 cut(s) 287, 492
DinI GGCGCC 1 cut(s) 68
DpnI GATC 7 cut(s) 108, 182, 266, 271, 458, 597, 794
DpnII GATC 7 cut(s) 106, 180, 264, 269, 456, 595, 792
DrdI GACNNNNNNGTC 1 cut(s) 251
DseDI GACNNNNNNGTC 1 cut(s) 251
Eam1104I CTCTTC 1 cut(s) 653
EarI CTCTTC 1 cut(s) 653
EciI GGCGGA 2 cut(s) 28, 257
Eco47I GGWCC 3 cut(s) 194, 295, 678
Eco57I CTGAAG 2 cut(s) 101, 753
Eco72I CACGTG 1 cut(s) 162
Eco88I CYCGRG 1 cut(s) 355
EcoNI CCTNNNNNAGG 1 cut(s) 358
EcoO109I RGGNCCY 3 cut(s) 194, 295, 363
EgeI GGCGCC 1 cut(s) 68
EheI GGCGCC 1 cut(s) 68
FaeI CATG 1 cut(s) 134
FaiI YATR 4 cut(s) 132, 501, 513, 609
FalI AAGNNNNNCTT 2 cut(s) 782, 814
FaqI GGGAC 3 cut(s) 180, 539, 691
FatI CATG 1 cut(s) 130
FauI CCCGC 4 cut(s) 291, 408, 445, 611
Fnu4HI GCNGC 4 cut(s) 547, 550, 564, 774
FokI GGATG 3 cut(s) 179, 215, 795
Fsp4HI GCNGC 4 cut(s) 547, 550, 564, 774
GlaI GCGC 4 cut(s) 68, 350, 441, 487
GluI GCNGC 4 cut(s) 547, 550, 564, 774
HaeII RGCGCY 2 cut(s) 70, 352
HaeIII GGCC 1 cut(s) 364
HapII CCGG 3 cut(s) 9, 681, 705
HgaI GACGC 1 cut(s) 541
HhaI GCGC 4 cut(s) 69, 351, 442, 488
Hin1I GRCGYC 2 cut(s) 67, 252
Hin1II CATG 1 cut(s) 134
Hin6I GCGC 4 cut(s) 67, 349, 440, 486
HinP1I GCGC 4 cut(s) 67, 349, 440, 486
HinfI GANTC 4 cut(s) 152, 172, 206, 601
HpaII CCGG 3 cut(s) 9, 681, 705
HphI GGTGA 1 cut(s) 158
Hpy166II GTNNAC 4 cut(s) 136, 223, 331, 719
Hpy188I TCNGA 3 cut(s) 269, 600, 643
Hpy188III TCNNGA 3 cut(s) 178, 474, 676
Hpy8I GTNNAC 4 cut(s) 136, 223, 331, 719
Hpy99I CGWCG 3 cut(s) 203, 469, 643
HpyAV CCTTC 2 cut(s) 646, 728
HpyCH4III ACNGT 5 cut(s) 146, 324, 394, 465, 575
HpyCH4IV ACGT 6 cut(s) 57, 161, 234, 252, 306, 747
HpyCH4V TGCA 2 cut(s) 566, 773
HpyF10VI GCNNNNNNNGC 3 cut(s) 521, 695, 770
HpyF3I CTNAG 2 cut(s) 287, 492
HpySE526I ACGT 6 cut(s) 57, 161, 234, 252, 306, 747
Hsp92I GRCGYC 2 cut(s) 67, 252
Hsp92II CATG 1 cut(s) 134
HspAI GCGC 4 cut(s) 67, 349, 440, 486
KasI GGCGCC 1 cut(s) 66
KflI GGGWCCC 1 cut(s) 194
Kzo9I GATC 7 cut(s) 106, 180, 264, 269, 456, 595, 792
LguI GCTCTTC 1 cut(s) 653
LmnI GCTCC 2 cut(s) 128, 695
LpnPI CCDG 6 cut(s) 22, 432, 534, 552, 694, 718
Lsp1109I GCAGC 4 cut(s) 550, 558, 561, 785
LweI GCATC 1 cut(s) 760
MaeII ACGT 6 cut(s) 57, 161, 234, 252, 306, 747
MaeIII GTNAC 6 cut(s) 25, 146, 186, 388, 407, 575
MalI GATC 7 cut(s) 108, 182, 266, 271, 458, 597, 794
MboI GATC 7 cut(s) 106, 180, 264, 269, 456, 595, 792
MboII GAAGA 4 cut(s) 27, 670, 787, 790
MflI RGATCY 3 cut(s) 106, 269, 792
MhlI GDGCHC 1 cut(s) 322
MluCI AATT 3 cut(s) 30, 61, 502
Mly113I GGCGCC 1 cut(s) 67
MlyI GAGTC 4 cut(s) 146, 166, 200, 610
MmeI TCCRAC 2 cut(s) 357, 621
MslI CAYNNNNRTG 1 cut(s) 131
MspA1I CMGCKG 1 cut(s) 776
MspI CCGG 3 cut(s) 9, 681, 705
MvnI CGCG 1 cut(s) 440
MwoI GCNNNNNNNGC 3 cut(s) 521, 695, 770
NarI GGCGCC 1 cut(s) 67
NdeII GATC 7 cut(s) 106, 180, 264, 269, 456, 595, 792
NlaIII CATG 1 cut(s) 134
NlaIV GGNNCC 9 cut(s) 68, 108, 195, 196, 271, 321, 365, 679, 697
NmuCI GTSAC 3 cut(s) 25, 146, 388
OliI CACNNNNGTG 1 cut(s) 131
PciSI GCTCTTC 1 cut(s) 653
PcsI WCGNNNNNNNCGW 2 cut(s) 403, 647
Pfl23II CGTACG 1 cut(s) 624
PkrI GCNGC 4 cut(s) 548, 551, 565, 775
PleI GAGTC 4 cut(s) 146, 166, 200, 609
PluTI GGCGCC 1 cut(s) 70
PmaCI CACGTG 1 cut(s) 162
PmlI CACGTG 1 cut(s) 162
PpsI GAGTC 4 cut(s) 146, 166, 200, 609
Ppu21I YACGTR 2 cut(s) 162, 748
PpuMI RGGWCCY 2 cut(s) 194, 295
Psp5II RGGWCCY 2 cut(s) 194, 295
PspCI CACGTG 1 cut(s) 162
PspLI CGTACG 1 cut(s) 624
PspN4I GGNNCC 9 cut(s) 68, 108, 195, 196, 271, 321, 365, 679, 697
PspPI GGNCC 4 cut(s) 194, 295, 363, 678
PspPPI RGGWCCY 2 cut(s) 194, 295
PstI CTGCAG 1 cut(s) 568
PsuI RGATCY 3 cut(s) 106, 269, 792
RsaI GTAC 5 cut(s) 452, 572, 626, 632, 817
RsaNI GTAC 5 cut(s) 451, 571, 625, 631, 816
RseI CAYNNNNRTG 1 cut(s) 131
SapI GCTCTTC 1 cut(s) 653
SatI GCNGC 4 cut(s) 547, 550, 564, 774
Sau3AI GATC 7 cut(s) 106, 180, 264, 269, 456, 595, 792
Sau96I GGNCC 4 cut(s) 194, 295, 363, 678
SchI GAGTC 4 cut(s) 146, 166, 200, 610
SduI GDGCHC 1 cut(s) 322
SfaNI GCATC 1 cut(s) 760
SfcI CTRYAG 1 cut(s) 564
SfoI GGCGCC 1 cut(s) 68
SinI GGWCC 3 cut(s) 194, 295, 678
SmiMI CAYNNNNRTG 1 cut(s) 131
SmlI CTYRAG 1 cut(s) 344
SmoI CTYRAG 1 cut(s) 344
Sse9I AATT 3 cut(s) 30, 61, 502
SsiI CCGC 8 cut(s) 39, 242, 273, 284, 401, 438, 618, 776
SspDI GGCGCC 1 cut(s) 66
SspI AATATT 1 cut(s) 536
TaaI ACNGT 5 cut(s) 146, 324, 394, 465, 575
TaiI ACGT 6 cut(s) 60, 164, 237, 255, 309, 750
TaqI TCGA 5 cut(s) 179, 201, 209, 300, 473
TasI AATT 3 cut(s) 30, 61, 502
TseFI GTSAC 3 cut(s) 25, 146, 388
TseI GCWGC 4 cut(s) 546, 549, 563, 773
Tsp45I GTSAC 3 cut(s) 25, 146, 388
TspGWI ACGGA 2 cut(s) 87, 316
VpaK11BI GGWCC 3 cut(s) 194, 295, 678
XagI CCTNNNNNAGG 1 cut(s) 358
ZraI GACGTC 1 cut(s) 253
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.