Rroxscaffold_7G00214240

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0004046
Physical Location & Seq
Reverse (-)
64842052 .. 64842877
826 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00214240.1

Sequence Viewer

Length: 723 bp
ATGGCTTCCGGCTACGAAGTAGAGGTCACAATTTCTTCCGCCAAAGACCTCAAAAACGTGAATTGGCGCCACGGAGAGCTGAAGCCCTACGCTGTTGTTTGGGTGGATCCAAACAATAAATGCTCGACCCACGTAGACGAATACGGCGACGACTCGCCCACGTGGGATGAGACGCTCTCGATCCCGTTACGGGGTTCCGTCGAGGACTCGACCCTTTTCGTGGACATCATCCACGTCAAGGCGGACGAGGACGTCAAGCCGCCCCACGGGAAGTTGGAAGTCAAAGTCACAGTTCGCCCGCCCCGTTACCAAGCACCTGACCCGTATCACGCCCCCGCGCCCTACGGGTACGGGATCCCACCGCCGCAGTCGAGAGACTACGGCGGACCTCAGCAGTATAATTATCAATATGCCCCTCCTGCCCAACCCCAATATTACCAAAGCAGCAGCGTCCCACAAGCAGGGTACGGTTACAATCAGCCATCTTACGATCAGAGTCAGTATGGGAGTGGCGGGTCGTACGGGTACGGGTCGTCGGAGAAGCCGAAGGAGAAGAGCAAGTTTGGGGTCGGGACCGGATTGGCTGTGGGAGCCGTAGCCGGAGTGTTGGGTGGACTTGCGATTGCTGAAGGAGTGGATTACGTGGAGGACAAGATTGCTGATGATGCAGCGGAGAAAGTGGAGGAGGATCTTGGTTATGATGATTTTGGCGATGATGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

240

Amino Acids

26.11

Weight (kDa)

4.47

Isoelectric Point (pI)

34.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 7 - 81 4.2e-08 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 251
AatII GACGTC 1 cut(s) 255
AccB1I GGYRCC 1 cut(s) 66
AccI GTMKAC 1 cut(s) 135
AccII CGCG 1 cut(s) 338
AclWI GGATC 6 cut(s) 101, 114, 175, 349, 362, 696
AcuI CTGAAG 2 cut(s) 101, 648
AcvI CACGTG 1 cut(s) 162
AcyI GRCGYC 2 cut(s) 67, 252
AfaI GTAC 4 cut(s) 350, 467, 521, 527
AfiI CCNNNNNNNGG 6 cut(s) 190, 191, 220, 238, 266, 461
AjiI CACGTC 1 cut(s) 235
AluBI AGCT 1 cut(s) 79
AluI AGCT 1 cut(s) 79
Alw26I GTCTC 2 cut(s) 164, 369
AlwI GGATC 6 cut(s) 101, 114, 175, 349, 362, 696
ApeKI GCWGC 3 cut(s) 444, 447, 668
ArsI GACNNNNNNTTYG 2 cut(s) 9, 41
AspLEI GCGC 2 cut(s) 69, 340
AspS9I GGNCC 2 cut(s) 386, 573
AvaII GGWCC 2 cut(s) 386, 573
BamHI GGATCC 2 cut(s) 106, 354
BanI GGYRCC 1 cut(s) 66
BbrPI CACGTG 1 cut(s) 162
BbvCI CCTCAGC 1 cut(s) 390
BbvI GCAGC 3 cut(s) 456, 459, 680
BccI CCATC 1 cut(s) 490
BceAI ACGGC 3 cut(s) 160, 397, 578
BcoDI GTCTC 2 cut(s) 164, 369
BfoI RGCGCY 1 cut(s) 70
BisI GCNGC 5 cut(s) 260, 365, 445, 448, 669
BlsI GCNGC 5 cut(s) 261, 366, 446, 449, 670
Bme18I GGWCC 2 cut(s) 386, 573
BmgBI CACGTC 1 cut(s) 235
BmgT120I GGNCC 2 cut(s) 386, 573
BmiI GGNNCC 6 cut(s) 68, 108, 196, 356, 574, 592
BmsI GCATC 1 cut(s) 655
BplI GAGNNNNNCTC 2 cut(s) 161, 193
Bpu10I CCTNAGC 1 cut(s) 390
BsaAI YACGTR 3 cut(s) 133, 162, 643
BsaHI GRCGYC 2 cut(s) 67, 252
BsaJI CCNNGG 2 cut(s) 70, 265
BsaWI WCCGGW 1 cut(s) 575
Bsc4I CCNNNNNNNGG 6 cut(s) 190, 191, 220, 238, 266, 461
BseDI CCNNGG 2 cut(s) 70, 265
BseGI GGATG 2 cut(s) 172, 228
BseLI CCNNNNNNNGG 6 cut(s) 190, 191, 220, 238, 266, 461
BseMII CTCAG 1 cut(s) 404
BseRI GAGGAG 1 cut(s) 698
BseXI GCAGC 3 cut(s) 456, 459, 680
Bsh1236I CGCG 1 cut(s) 338
BshNI GGYRCC 1 cut(s) 66
BsiSI CCGG 3 cut(s) 9, 576, 600
BsiWI CGTACG 1 cut(s) 519
BslFI GGGAC 2 cut(s) 437, 586
BslI CCNNNNNNNGG 6 cut(s) 190, 191, 220, 238, 266, 461
BsmAI GTCTC 2 cut(s) 164, 369
BsmBI CGTCTC 1 cut(s) 164
BsmFI GGGAC 2 cut(s) 437, 586
Bsp143I GATC 5 cut(s) 106, 180, 354, 490, 688
BspCNI CTCAG 1 cut(s) 403
BspFNI CGCG 1 cut(s) 338
BspLI GGNNCC 6 cut(s) 68, 108, 196, 356, 574, 592
BspPI GGATC 6 cut(s) 101, 114, 175, 349, 362, 696
BspQI GCTCTTC 1 cut(s) 548
BspT107I GGYRCC 1 cut(s) 66
BssECI CCNNGG 2 cut(s) 70, 265
BssMI GATC 5 cut(s) 106, 180, 354, 490, 688
BssNI GRCGYC 2 cut(s) 67, 252
Bst4CI ACNGT 2 cut(s) 292, 470
Bst6I CTCTTC 1 cut(s) 548
BstACI GRCGYC 2 cut(s) 67, 252
BstBAI YACGTR 3 cut(s) 133, 162, 643
BstC8I GCNNGC 1 cut(s) 299
BstDEI CTNAG 1 cut(s) 390
BstDSI CCRYGG 2 cut(s) 70, 265
BstF5I GGATG 2 cut(s) 172, 228
BstFNI CGCG 1 cut(s) 338
BstH2I RGCGCY 1 cut(s) 70
BstHHI GCGC 2 cut(s) 69, 340
BstKTI GATC 5 cut(s) 109, 183, 357, 493, 691
BstMAI GTCTC 2 cut(s) 164, 369
BstMBI GATC 5 cut(s) 106, 180, 354, 490, 688
BstMWI GCNNNNNNNGC 3 cut(s) 419, 590, 665
BstUI CGCG 1 cut(s) 338
BstV1I GCAGC 3 cut(s) 456, 459, 680
BstX2I RGATCY 3 cut(s) 106, 354, 688
BstYI RGATCY 3 cut(s) 106, 354, 688
BtgI CCRYGG 2 cut(s) 70, 265
BtrI CACGTC 1 cut(s) 235
BtsCI GGATG 2 cut(s) 172, 228
Cac8I GCNNGC 1 cut(s) 299
CfoI GCGC 2 cut(s) 69, 340
Cfr13I GGNCC 2 cut(s) 386, 573
CseI GACGC 2 cut(s) 181, 439
Csp6I GTAC 4 cut(s) 349, 466, 520, 526
CviQI GTAC 4 cut(s) 349, 466, 520, 526
DdeI CTNAG 1 cut(s) 390
DinI GGCGCC 1 cut(s) 68
DpnI GATC 5 cut(s) 108, 182, 356, 492, 690
DpnII GATC 5 cut(s) 106, 180, 354, 490, 688
DrdI GACNNNNNNGTC 1 cut(s) 251
DseDI GACNNNNNNGTC 1 cut(s) 251
Eam1104I CTCTTC 1 cut(s) 548
EarI CTCTTC 1 cut(s) 548
EciI GGCGGA 3 cut(s) 28, 257, 399
Eco47I GGWCC 2 cut(s) 386, 573
Eco57I CTGAAG 2 cut(s) 101, 648
Eco72I CACGTG 1 cut(s) 162
EgeI GGCGCC 1 cut(s) 68
EheI GGCGCC 1 cut(s) 68
Esp3I CGTCTC 1 cut(s) 164
FaiI YATR 4 cut(s) 399, 411, 504, 699
FaqI GGGAC 2 cut(s) 437, 586
FauI CCCGC 3 cut(s) 306, 343, 506
FblI GTMKAC 1 cut(s) 135
Fnu4HI GCNGC 5 cut(s) 260, 365, 445, 448, 669
FokI GGATG 2 cut(s) 179, 215
Fsp4HI GCNGC 5 cut(s) 260, 365, 445, 448, 669
GlaI GCGC 2 cut(s) 68, 339
GluI GCNGC 5 cut(s) 260, 365, 445, 448, 669
HaeII RGCGCY 1 cut(s) 70
HapII CCGG 3 cut(s) 9, 576, 600
HgaI GACGC 2 cut(s) 181, 439
HhaI GCGC 2 cut(s) 69, 340
Hin1I GRCGYC 2 cut(s) 67, 252
Hin6I GCGC 2 cut(s) 67, 338
HinP1I GCGC 2 cut(s) 67, 338
HinfI GANTC 3 cut(s) 152, 206, 496
HpaII CCGG 3 cut(s) 9, 576, 600
Hpy166II GTNNAC 3 cut(s) 136, 223, 614
Hpy188I TCNGA 2 cut(s) 495, 538
Hpy188III TCNNGA 3 cut(s) 178, 372, 571
Hpy8I GTNNAC 3 cut(s) 136, 223, 614
Hpy99I CGWCG 3 cut(s) 152, 203, 538
HpyAV CCTTC 2 cut(s) 541, 623
HpyCH4III ACNGT 2 cut(s) 292, 470
HpyCH4IV ACGT 6 cut(s) 57, 132, 161, 234, 252, 642
HpyCH4V TGCA 1 cut(s) 668
HpyF10VI GCNNNNNNNGC 3 cut(s) 419, 590, 665
HpyF3I CTNAG 1 cut(s) 390
HpySE526I ACGT 6 cut(s) 57, 132, 161, 234, 252, 642
Hsp92I GRCGYC 2 cut(s) 67, 252
HspAI GCGC 2 cut(s) 67, 338
KasI GGCGCC 1 cut(s) 66
Kzo9I GATC 5 cut(s) 106, 180, 354, 490, 688
LguI GCTCTTC 1 cut(s) 548
LmnI GCTCC 1 cut(s) 590
LpnPI CCDG 6 cut(s) 22, 330, 432, 447, 589, 613
Lsp1109I GCAGC 3 cut(s) 456, 459, 680
LweI GCATC 1 cut(s) 655
MaeII ACGT 6 cut(s) 57, 132, 161, 234, 252, 642
MaeIII GTNAC 5 cut(s) 25, 186, 286, 305, 470
MalI GATC 5 cut(s) 108, 182, 356, 492, 690
MboI GATC 5 cut(s) 106, 180, 354, 490, 688
MboII GAAGA 2 cut(s) 27, 565
MflI RGATCY 3 cut(s) 106, 354, 688
MluCI AATT 3 cut(s) 30, 61, 400
Mly113I GGCGCC 1 cut(s) 67
MlyI GAGTC 3 cut(s) 146, 200, 505
MmeI TCCRAC 2 cut(s) 255, 516
MnlI CCTC 9 cut(s) 16, 59, 196, 241, 399, 426, 640, 676, 679
MspA1I CMGCKG 1 cut(s) 671
MspI CCGG 3 cut(s) 9, 576, 600
MvnI CGCG 1 cut(s) 338
MwoI GCNNNNNNNGC 3 cut(s) 419, 590, 665
NarI GGCGCC 1 cut(s) 67
NdeII GATC 5 cut(s) 106, 180, 354, 490, 688
NlaIV GGNNCC 6 cut(s) 68, 108, 196, 356, 574, 592
NmuCI GTSAC 2 cut(s) 25, 286
PciSI GCTCTTC 1 cut(s) 548
PcsI WCGNNNNNNNCGW 3 cut(s) 144, 301, 542
Pfl23II CGTACG 1 cut(s) 519
PkrI GCNGC 5 cut(s) 261, 366, 446, 449, 670
PleI GAGTC 3 cut(s) 146, 200, 504
PluTI GGCGCC 1 cut(s) 70
PmaCI CACGTG 1 cut(s) 162
PmlI CACGTG 1 cut(s) 162
PpsI GAGTC 3 cut(s) 146, 200, 504
Ppu21I YACGTR 3 cut(s) 133, 162, 643
PspCI CACGTG 1 cut(s) 162
PspLI CGTACG 1 cut(s) 519
PspN4I GGNNCC 6 cut(s) 68, 108, 196, 356, 574, 592
PspPI GGNCC 2 cut(s) 386, 573
PsuI RGATCY 3 cut(s) 106, 354, 688
RsaI GTAC 4 cut(s) 350, 467, 521, 527
RsaNI GTAC 4 cut(s) 349, 466, 520, 526
SapI GCTCTTC 1 cut(s) 548
SatI GCNGC 5 cut(s) 260, 365, 445, 448, 669
Sau3AI GATC 5 cut(s) 106, 180, 354, 490, 688
Sau96I GGNCC 2 cut(s) 386, 573
SchI GAGTC 3 cut(s) 146, 200, 505
SfaNI GCATC 1 cut(s) 655
SfoI GGCGCC 1 cut(s) 68
SinI GGWCC 2 cut(s) 386, 573
Sse9I AATT 3 cut(s) 30, 61, 400
SspDI GGCGCC 1 cut(s) 66
SspI AATATT 1 cut(s) 434
TaaI ACNGT 2 cut(s) 292, 470
TaiI ACGT 6 cut(s) 60, 135, 164, 237, 255, 645
TaqI TCGA 5 cut(s) 125, 179, 201, 209, 371
TasI AATT 3 cut(s) 30, 61, 400
TauI GCSGC 2 cut(s) 262, 367
TseFI GTSAC 2 cut(s) 25, 286
TseI GCWGC 3 cut(s) 444, 447, 668
Tsp45I GTSAC 2 cut(s) 25, 286
TspGWI ACGGA 2 cut(s) 87, 187
VpaK11BI GGWCC 2 cut(s) 386, 573
XmiI GTMKAC 1 cut(s) 135
ZraI GACGTC 1 cut(s) 253
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.