Prupe.2G298800_v2.0.a1

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0004046
Physical Location & Seq
Reverse (-)
28815293 .. 28816330
1038 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G298800.1

Sequence Viewer

Length: 867 bp
ATGGCGTCCGGCCACGAAGTCCAAGTCAAGCTCTCCTCCGCCCGCGACCTCAAGAACGTCAACTGGCGAAACGGCCCAGTGAAGCCGTACGCCGTCGTATGGGTCGACCCCAAGAACAAGTGCTCCACCAGGGTCGACGAGGAGGGTGACACGTCACCTTATTGGGACGAGACGCTCACCATCCCTCTACCGGGTCCTGTCGACGGCGACACCACGCTCTACATTGACGTCGTGCACGCCGGATCCGACCCTGACACCAAGCCACTGATCGGGTCCGCCCGGCTCAAACTCCGCGAAGTCATCGATGACGTGGGCTTCGACGAGCGCGCCAGCCGCACTCTGCAGCTCAAGCGACCCTCTGGCAGGCCCCAGGGCAAGGTTGACGTTAAGGTTACTATTAGGGAGCCGCGCTACCGTGCGCCGGAGCCGTACTACGCTCCGCCTTATGGGGTCCCACCGGCGGGGTCGAGAGATTATACTGCACCGCCGCCGGCGTATGGCGCACCGTACGCCGCACCCGCACCGCCGCCGACTTATGGAGTACCATACGCCACACCTGCACCGCCGCCTCCGCGCGATTACTACGCGGCCCCACCTTCTGGGTACCAGTACAGTGCGTACAATGCTCCTCCAGCGCCGTACGGTCAGCCGGCTCAGGCGAGTTATGGTCAGGCGGGGTATGGACATGAGGAGAAGAAGAGTAAGTTTGGTGGGATGGGGACAGGATTGGCTGTGGGCGCGGTGGCGGGGGTTCTTGGGGGAGTCGCATTGGCGGAGGGCTTTGATTACGTGGAGGACAAAATTGCGGATGACGTGGCGGAGAAGGTGGAAGATGATATTGGGTACGACGATGGGGATGACTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

289

Amino Acids

30.86

Weight (kDa)

4.81

Isoelectric Point (pI)

39.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 565
AatII GACGTC 1 cut(s) 231
Acc36I ACCTGC 1 cut(s) 565
Acc65I GGTACC 1 cut(s) 603
AccB1I GGYRCC 1 cut(s) 603
AccB7I CCANNNNNTGG 1 cut(s) 599
AccI GTMKAC 3 cut(s) 105, 135, 201
AccII CGCG 8 cut(s) 45, 294, 327, 409, 574, 576, 587, 740
AclWI GGATC 2 cut(s) 237, 250
AcoI YGGCCR 1 cut(s) 10
AcyI GRCGYC 2 cut(s) 5, 228
AfaI GTAC 9 cut(s) 89, 431, 509, 543, 605, 611, 620, 641, 845
AflIII ACRYGT 1 cut(s) 150
AjiI CACGTC 3 cut(s) 153, 310, 814
AjnI CCWGG 2 cut(s) 128, 369
AjuI GAANNNNNNNTTGG 2 cut(s) 822, 854
AloI GAACNNNNNNTCC 2 cut(s) 107, 139
AluBI AGCT 2 cut(s) 31, 346
AluI AGCT 2 cut(s) 31, 346
Alw21I GWGCWC 2 cut(s) 125, 237
Alw26I GTCTC 1 cut(s) 164
Alw44I GTGCAC 1 cut(s) 233
AlwI GGATC 2 cut(s) 237, 250
AoxI GGCC 4 cut(s) 10, 73, 365, 588
ApaLI GTGCAC 1 cut(s) 233
ApeKI GCWGC 1 cut(s) 343
ArsI GACNNNNNNTTYG 4 cut(s) 9, 41, 299, 331
Asp718I GGTACC 1 cut(s) 603
AspLEI GCGC 8 cut(s) 327, 329, 411, 421, 503, 576, 637, 740
AspS9I GGNCC 6 cut(s) 74, 194, 273, 366, 451, 589
AsuC2I CCSGG 2 cut(s) 192, 280
AsuHPI GGTGA 3 cut(s) 147, 158, 169
AvaII GGWCC 3 cut(s) 194, 273, 451
BaeGI GKGCMC 1 cut(s) 237
BamHI GGATCC 1 cut(s) 242
BanI GGYRCC 1 cut(s) 603
Bbv12I GWGCWC 2 cut(s) 125, 237
BbvI GCAGC 1 cut(s) 355
BccI CCATC 3 cut(s) 188, 709, 845
BceAI ACGGC 6 cut(s) 70, 77, 88, 220, 412, 622
BciT130I CCWGG 2 cut(s) 130, 371
BcnI CCSGG 2 cut(s) 192, 280
BcoDI GTCTC 1 cut(s) 164
BfaI CTAG 1 cut(s) 865
BfmI CTRYAG 1 cut(s) 341
BfoI RGCGCY 1 cut(s) 638
BfuAI ACCTGC 1 cut(s) 565
BisI GCNGC 8 cut(s) 334, 344, 407, 488, 513, 527, 566, 588
BlsI GCNGC 8 cut(s) 335, 345, 408, 489, 514, 528, 567, 589
Bme1390I CCNGG 4 cut(s) 130, 192, 280, 371
Bme18I GGWCC 3 cut(s) 194, 273, 451
BmgBI CACGTC 3 cut(s) 153, 310, 814
BmgT120I GGNCC 6 cut(s) 74, 194, 273, 366, 451, 589
BmrFI CCNGG 4 cut(s) 130, 192, 280, 371
BmrI ACTGGG 1 cut(s) 71
BmuI ACTGGG 1 cut(s) 71
BpmI CTGGAG 1 cut(s) 615
Bpu10I CCTNAGC 1 cut(s) 654
BpuEI CTTGAG 2 cut(s) 35, 332
BpuMI CCSGG 2 cut(s) 192, 280
Bsa29I ATCGAT 1 cut(s) 303
BsaAI YACGTR 1 cut(s) 790
BsaHI GRCGYC 2 cut(s) 5, 228
BsaJI CCNNGG 3 cut(s) 129, 369, 370
BsaXI ACNNNNNCTCC 2 cut(s) 107, 137
Bse118I RCCGGY 3 cut(s) 457, 490, 649
Bse1I ACTGG 3 cut(s) 68, 77, 607
BseBI CCWGG 2 cut(s) 130, 371
BseCI ATCGAT 1 cut(s) 303
BseDI CCNNGG 3 cut(s) 129, 369, 370
BseGI GGATG 4 cut(s) 180, 720, 814, 862
BseMII CTCAG 1 cut(s) 668
BseNI ACTGG 3 cut(s) 68, 77, 607
BsePI GCGCGC 1 cut(s) 325
BseRI GAGGAG 4 cut(s) 25, 155, 618, 704
BseSI GKGCMC 1 cut(s) 237
BseXI GCAGC 1 cut(s) 355
BsgI GTGCAG 2 cut(s) 465, 543
Bsh1236I CGCG 8 cut(s) 45, 294, 327, 409, 574, 576, 587, 740
BshFI GGCC 4 cut(s) 12, 75, 367, 590
BshNI GGYRCC 1 cut(s) 603
BshVI ATCGAT 1 cut(s) 303
BsiHKAI GWGCWC 2 cut(s) 125, 237
BsiSI CCGG 8 cut(s) 9, 191, 240, 280, 422, 458, 491, 650
BsiWI CGTACG 3 cut(s) 87, 507, 639
BslFI GGGAC 3 cut(s) 179, 437, 733
BsmAI GTCTC 1 cut(s) 164
BsmBI CGTCTC 1 cut(s) 164
BsmFI GGGAC 3 cut(s) 179, 437, 733
BsnI GGCC 4 cut(s) 12, 75, 367, 590
Bsp1286I GDGCHC 2 cut(s) 125, 237
Bsp143I GATC 2 cut(s) 242, 267
BspANI GGCC 4 cut(s) 12, 75, 367, 590
BspCNI CTCAG 1 cut(s) 667
BspDI ATCGAT 1 cut(s) 303
BspFNI CGCG 8 cut(s) 45, 294, 327, 409, 574, 576, 587, 740
BspMAI CTGCAG 1 cut(s) 345
BspMI ACCTGC 1 cut(s) 565
BspPI GGATC 2 cut(s) 237, 250
BspT107I GGYRCC 1 cut(s) 603
BsrFI RCCGGY 3 cut(s) 457, 490, 649
BsrI ACTGG 3 cut(s) 68, 77, 607
BssAI RCCGGY 3 cut(s) 457, 490, 649
BssECI CCNNGG 3 cut(s) 129, 369, 370
BssHII GCGCGC 1 cut(s) 325
BssMI GATC 2 cut(s) 242, 267
BssNI GRCGYC 2 cut(s) 5, 228
Bst2UI CCWGG 2 cut(s) 130, 371
Bst4CI ACNGT 4 cut(s) 416, 507, 614, 644
Bst6I CTCTTC 1 cut(s) 692
BstACI GRCGYC 2 cut(s) 5, 228
BstBAI YACGTR 1 cut(s) 790
BstC8I GCNNGC 7 cut(s) 43, 237, 327, 331, 365, 492, 651
BstDEI CTNAG 1 cut(s) 654
BstENI CCTNNNNNAGG 1 cut(s) 361
BstF5I GGATG 4 cut(s) 180, 720, 814, 862
BstFNI CGCG 8 cut(s) 45, 294, 327, 409, 574, 576, 587, 740
BstH2I RGCGCY 1 cut(s) 638
BstHHI GCGC 8 cut(s) 327, 329, 411, 421, 503, 576, 637, 740
BstKTI GATC 2 cut(s) 245, 270
BstMAI GTCTC 1 cut(s) 164
BstMBI GATC 2 cut(s) 242, 267
BstNI CCWGG 2 cut(s) 130, 371
BstSCI CCNGG 4 cut(s) 128, 190, 278, 369
BstSFI CTRYAG 1 cut(s) 341
BstSLI GKGCMC 1 cut(s) 237
BstUI CGCG 8 cut(s) 45, 294, 327, 409, 574, 576, 587, 740
BstV1I GCAGC 1 cut(s) 355
BstX2I RGATCY 1 cut(s) 242
BstYI RGATCY 1 cut(s) 242
Bsu15I ATCGAT 1 cut(s) 303
BsuRI GGCC 4 cut(s) 12, 75, 367, 590
BsuTUI ATCGAT 1 cut(s) 303
BtrI CACGTC 3 cut(s) 153, 310, 814
BtsCI GGATG 4 cut(s) 180, 720, 814, 862
BtsIMutI CAGTG 3 cut(s) 84, 263, 619
BveI ACCTGC 1 cut(s) 565
Cac8I GCNNGC 7 cut(s) 43, 237, 327, 331, 365, 492, 651
CfoI GCGC 8 cut(s) 327, 329, 411, 421, 503, 576, 637, 740
Cfr10I RCCGGY 3 cut(s) 457, 490, 649
Cfr13I GGNCC 6 cut(s) 74, 194, 273, 366, 451, 589
ClaI ATCGAT 1 cut(s) 303
CseI GACGC 1 cut(s) 181
Csp6I GTAC 9 cut(s) 88, 430, 508, 542, 604, 610, 619, 640, 844
CviAII CATG 1 cut(s) 686
CviQI GTAC 9 cut(s) 88, 430, 508, 542, 604, 610, 619, 640, 844
DdeI CTNAG 1 cut(s) 654
DpnI GATC 2 cut(s) 244, 269
DpnII GATC 2 cut(s) 242, 267
EaeI YGGCCR 1 cut(s) 10
Eam1104I CTCTTC 1 cut(s) 692
EarI CTCTTC 1 cut(s) 692
EciI GGCGGA 5 cut(s) 28, 265, 429, 788, 833
Eco47I GGWCC 3 cut(s) 194, 273, 451
EcoNI CCTNNNNNAGG 1 cut(s) 361
EcoO109I RGGNCCY 3 cut(s) 194, 366, 451
EcoRII CCWGG 2 cut(s) 128, 369
Esp3I CGTCTC 1 cut(s) 164
FaeI CATG 1 cut(s) 689
FaiI YATR 9 cut(s) 100, 447, 477, 498, 537, 547, 666, 681, 687
FaqI GGGAC 3 cut(s) 179, 437, 733
FatI CATG 1 cut(s) 685
FauI CCCGC 5 cut(s) 50, 454, 526, 667, 739
FblI GTMKAC 3 cut(s) 105, 135, 201
Fnu4HI GCNGC 8 cut(s) 334, 344, 407, 488, 513, 527, 566, 588
FokI GGATG 3 cut(s) 167, 727, 821
Fsp4HI GCNGC 8 cut(s) 334, 344, 407, 488, 513, 527, 566, 588
FspBI CTAG 1 cut(s) 865
GlaI GCGC 8 cut(s) 326, 328, 410, 420, 502, 575, 636, 739
GluI GCNGC 8 cut(s) 334, 344, 407, 488, 513, 527, 566, 588
GsuI CTGGAG 1 cut(s) 615
HaeII RGCGCY 1 cut(s) 638
HaeIII GGCC 4 cut(s) 12, 75, 367, 590
HapII CCGG 8 cut(s) 9, 191, 240, 280, 422, 458, 491, 650
HgaI GACGC 1 cut(s) 181
HhaI GCGC 8 cut(s) 327, 329, 411, 421, 503, 576, 637, 740
Hin1I GRCGYC 2 cut(s) 5, 228
Hin1II CATG 1 cut(s) 689
Hin6I GCGC 8 cut(s) 325, 327, 409, 419, 501, 574, 635, 738
HinP1I GCGC 8 cut(s) 325, 327, 409, 419, 501, 574, 635, 738
HincII GTYRAC 5 cut(s) 61, 106, 136, 202, 382
HindII GTYRAC 5 cut(s) 61, 106, 136, 202, 382
HinfI GANTC 1 cut(s) 762
HpaII CCGG 8 cut(s) 9, 191, 240, 280, 422, 458, 491, 650
HphI GGTGA 3 cut(s) 147, 158, 169
Hpy166II GTNNAC 6 cut(s) 61, 106, 136, 202, 235, 382
Hpy188I TCNGA 1 cut(s) 247
Hpy188III TCNNGA 2 cut(s) 52, 468
Hpy8I GTNNAC 6 cut(s) 61, 106, 136, 202, 235, 382
Hpy99I CGWCG 6 cut(s) 98, 140, 206, 233, 323, 851
HpyAV CCTTC 2 cut(s) 606, 817
HpyCH4III ACNGT 4 cut(s) 416, 507, 614, 644
HpyCH4IV ACGT 7 cut(s) 57, 152, 228, 309, 384, 789, 813
HpyCH4V TGCA 4 cut(s) 235, 343, 482, 560
HpyF3I CTNAG 1 cut(s) 654
HpySE526I ACGT 7 cut(s) 57, 152, 228, 309, 384, 789, 813
Hsp92I GRCGYC 2 cut(s) 5, 228
Hsp92II CATG 1 cut(s) 689
HspAI GCGC 8 cut(s) 325, 327, 409, 419, 501, 574, 635, 738
KflI GGGWCCC 1 cut(s) 451
KpnI GGTACC 1 cut(s) 607
KroI GCCGGC 2 cut(s) 490, 649
KroNI GCCGGC 2 cut(s) 492, 651
Kzo9I GATC 2 cut(s) 242, 267
LmnI GCTCC 5 cut(s) 128, 403, 424, 442, 631
Lsp1109I GCAGC 1 cut(s) 355
MaeI CTAG 1 cut(s) 865
MaeII ACGT 7 cut(s) 57, 152, 228, 309, 384, 789, 813
MaeIII GTNAC 3 cut(s) 146, 153, 391
MalI GATC 2 cut(s) 244, 269
MboI GATC 2 cut(s) 242, 267
MboII GAAGA 3 cut(s) 706, 709, 842
MflI RGATCY 1 cut(s) 242
MhlI GDGCHC 2 cut(s) 125, 237
MluCI AATT 1 cut(s) 801
MlyI GAGTC 1 cut(s) 771
MmeI TCCRAC 1 cut(s) 270
MreI CGCCGGCG 1 cut(s) 490
MroNI GCCGGC 2 cut(s) 490, 649
MseI TTAA 1 cut(s) 387
MspI CCGG 8 cut(s) 9, 191, 240, 280, 422, 458, 491, 650
MspR9I CCNGG 4 cut(s) 130, 192, 280, 371
MvaI CCWGG 2 cut(s) 130, 371
MvnI CGCG 8 cut(s) 45, 294, 327, 409, 574, 576, 587, 740
NaeI GCCGGC 2 cut(s) 492, 651
NciI CCSGG 2 cut(s) 192, 280
NdeII GATC 2 cut(s) 242, 267
NgoMIV GCCGGC 2 cut(s) 490, 649
NlaIII CATG 1 cut(s) 689
NmuCI GTSAC 2 cut(s) 146, 153
PaqCI CACCTGC 1 cut(s) 565
PasI CCCWGGG 1 cut(s) 370
PauI GCGCGC 1 cut(s) 325
PcsI WCGNNNNNNNCGW 3 cut(s) 102, 243, 315
PdiI GCCGGC 2 cut(s) 492, 651
Pfl23II CGTACG 3 cut(s) 87, 507, 639
PflMI CCANNNNNTGG 1 cut(s) 599
PkrI GCNGC 8 cut(s) 335, 345, 408, 489, 514, 528, 567, 589
PleI GAGTC 1 cut(s) 770
PpsI GAGTC 1 cut(s) 770
Ppu21I YACGTR 1 cut(s) 790
PpuMI RGGWCCY 2 cut(s) 194, 451
Psp5II RGGWCCY 2 cut(s) 194, 451
Psp6I CCWGG 2 cut(s) 128, 369
PspGI CCWGG 2 cut(s) 128, 369
PspLI CGTACG 3 cut(s) 87, 507, 639
PspPI GGNCC 6 cut(s) 74, 194, 273, 366, 451, 589
PspPPI RGGWCCY 2 cut(s) 194, 451
PstI CTGCAG 1 cut(s) 345
PsuI RGATCY 1 cut(s) 242
PteI GCGCGC 1 cut(s) 325
RsaI GTAC 9 cut(s) 89, 431, 509, 543, 605, 611, 620, 641, 845
RsaNI GTAC 9 cut(s) 88, 430, 508, 542, 604, 610, 619, 640, 844
SalI GTCGAC 3 cut(s) 104, 134, 200
SaqAI TTAA 1 cut(s) 387
SatI GCNGC 8 cut(s) 334, 344, 407, 488, 513, 527, 566, 588
Sau3AI GATC 2 cut(s) 242, 267
Sau96I GGNCC 6 cut(s) 74, 194, 273, 366, 451, 589
SchI GAGTC 1 cut(s) 771
ScrFI CCNGG 4 cut(s) 130, 192, 280, 371
SduI GDGCHC 2 cut(s) 125, 237
SfcI CTRYAG 1 cut(s) 341
SgrAI CRCCGGYG 2 cut(s) 457, 490
SinI GGWCC 3 cut(s) 194, 273, 451
SmlI CTYRAG 2 cut(s) 50, 347
SmoI CTYRAG 2 cut(s) 50, 347
Sse9I AATT 1 cut(s) 801
SspMI CTAG 1 cut(s) 865
StyD4I CCNGG 4 cut(s) 128, 190, 278, 369
TaaI ACNGT 4 cut(s) 416, 507, 614, 644
TaiI ACGT 7 cut(s) 60, 155, 231, 312, 387, 792, 816
TaqI TCGA 6 cut(s) 105, 135, 201, 303, 318, 467
TasI AATT 1 cut(s) 801
TatI WGTACW 1 cut(s) 609
TauI GCSGC 7 cut(s) 336, 409, 490, 515, 529, 568, 590
Tru1I TTAA 1 cut(s) 387
Tru9I TTAA 1 cut(s) 387
TscAI CASTG 3 cut(s) 84, 270, 619
TseFI GTSAC 2 cut(s) 146, 153
TseI GCWGC 1 cut(s) 343
Tsp45I GTSAC 2 cut(s) 146, 153
TspRI CASTG 3 cut(s) 84, 270, 619
Van91I CCANNNNNTGG 1 cut(s) 599
VneI GTGCAC 1 cut(s) 233
VpaK11BI GGWCC 3 cut(s) 194, 273, 451
XagI CCTNNNNNAGG 1 cut(s) 361
XmiI GTMKAC 3 cut(s) 105, 135, 201
XspI CTAG 1 cut(s) 865
ZraI GACGTC 1 cut(s) 229
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.