Rh6AG038500

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0004046
Physical Location & Seq
Forward (+)
5327937 .. 5329067
1131 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG038500.1

Sequence Viewer

Length: 786 bp
ATGGCTTCCGGCTACGAAGTAGAGGTCACAATTTCTTCCGCCAAAGACCTCAAAAACGTGAATTGGCGCCACGGAGAGCTGAAGCCCTACGCTGTTGTTTGGGTGGATCCAAACAATAAATGCTCCACCCACGTGGACGAATACGGCGACGACTCGCCCACGTGGGATGAGACGCTCTCGATCCCATTACGAGGTCCCGTCGAGGACTCGACCCTTTTCGTGGACATCATCCACGTCAAGGCGGACGAGGACGTCAAGCCGTTGATCGGATCCGCAAAACTCCCGCTTAGGGAGGTCCTCGATGACGTGGGGCTTGGGGCACCGTCCGTTCACAAGTTGAAGCTCAAGCGCCCCTCGGGTAGGCCCCACGGGAAGTTGGAAGTAAAAGTCACAGTTCGCCCGCCCCGTTACCAAGCACCTGACCCGTATCACGCCCCCGCGCCCTACGGGTACGGGATTGCACCGTCGCAGTCGAGAGACTACGGCGCACCTCAGCAGTATAATTATCAATATGCTCCTCCTGCCCAACCCCAATATTACCAAAGCAGCAGCGTCCCACAAGCAGGGTACGGTTACAATCAGCCATCTTACGATCAGAGTCAGTATGGGAGTGGCGGGTCGTACGGGTACGGGTCGTCGGAGAAGCCGAAGGAGAAGAGCAAGTTTGGGGTCGGGACCGGATTGGCTGTGGGAGCCGTAGCCGGAGTGTTGGGTGGACTTGCAATTGCCGAAGGAGTGGATTACGTGGAGGACAAGATTGCTGATGATGCAGCGGAGAAATGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

261

Amino Acids

28.3

Weight (kDa)

5.39

Isoelectric Point (pI)

35.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 7 - 100 1.3e-10 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 251
AatII GACGTC 1 cut(s) 255
AccB1I GGYRCC 2 cut(s) 66, 319
AccII CGCG 1 cut(s) 440
AciI CCGC 8 cut(s) 39, 242, 273, 284, 401, 438, 615, 773
AclWI GGATC 5 cut(s) 101, 114, 175, 264, 277
AcuI CTGAAG 1 cut(s) 101
AcvI CACGTG 2 cut(s) 133, 162
AcyI GRCGYC 2 cut(s) 67, 252
AfaI GTAC 4 cut(s) 452, 569, 623, 629
AfiI CCNNNNNNNGG 7 cut(s) 191, 220, 238, 266, 289, 360, 563
AgsI TTSAA 1 cut(s) 340
AjiI CACGTC 2 cut(s) 235, 307
AleI CACNNNNGTG 1 cut(s) 131
AluBI AGCT 2 cut(s) 79, 343
AluI AGCT 2 cut(s) 79, 343
Alw26I GTCTC 2 cut(s) 164, 471
AlwI GGATC 5 cut(s) 101, 114, 175, 264, 277
Ama87I CYCGRG 1 cut(s) 355
AoxI GGCC 1 cut(s) 362
ApeKI GCWGC 3 cut(s) 546, 549, 770
ArsI GACNNNNNNTTYG 2 cut(s) 9, 41
AspLEI GCGC 4 cut(s) 69, 351, 442, 488
AspS9I GGNCC 4 cut(s) 194, 295, 363, 675
AvaI CYCGRG 1 cut(s) 355
AvaII GGWCC 3 cut(s) 194, 295, 675
BaeGI GKGCMC 1 cut(s) 322
BamHI GGATCC 2 cut(s) 106, 269
BanI GGYRCC 2 cut(s) 66, 319
BbrPI CACGTG 2 cut(s) 133, 162
BbvCI CCTCAGC 1 cut(s) 492
BbvI GCAGC 3 cut(s) 558, 561, 782
BccI CCATC 1 cut(s) 592
BceAI ACGGC 4 cut(s) 160, 244, 499, 680
BcoDI GTCTC 2 cut(s) 164, 471
BfoI RGCGCY 2 cut(s) 70, 352
BisI GCNGC 3 cut(s) 547, 550, 771
BlsI GCNGC 3 cut(s) 548, 551, 772
Bme18I GGWCC 3 cut(s) 194, 295, 675
BmeT110I CYCGRG 1 cut(s) 355
BmgBI CACGTC 2 cut(s) 235, 307
BmgT120I GGNCC 4 cut(s) 194, 295, 363, 675
BmiI GGNNCC 8 cut(s) 68, 108, 196, 271, 321, 365, 676, 694
BmsI GCATC 1 cut(s) 757
BplI GAGNNNNNCTC 2 cut(s) 161, 193
Bpu10I CCTNAGC 2 cut(s) 287, 492
BpuEI CTTGAG 1 cut(s) 329
BsaAI YACGTR 3 cut(s) 133, 162, 745
BsaHI GRCGYC 2 cut(s) 67, 252
BsaJI CCNNGG 3 cut(s) 70, 354, 367
BsaWI WCCGGW 1 cut(s) 677
BsaXI ACNNNNNCTCC 1 cut(s) 767
Bsc4I CCNNNNNNNGG 7 cut(s) 191, 220, 238, 266, 289, 360, 563
BseDI CCNNGG 3 cut(s) 70, 354, 367
BseGI GGATG 2 cut(s) 172, 228
BseLI CCNNNNNNNGG 7 cut(s) 191, 220, 238, 266, 289, 360, 563
BseMII CTCAG 1 cut(s) 506
BseRI GAGGAG 1 cut(s) 507
BseSI GKGCMC 1 cut(s) 322
BseXI GCAGC 3 cut(s) 558, 561, 782
Bsh1236I CGCG 1 cut(s) 440
BshFI GGCC 1 cut(s) 364
BshNI GGYRCC 2 cut(s) 66, 319
BsiHKCI CYCGRG 1 cut(s) 355
BsiSI CCGG 3 cut(s) 9, 678, 702
BsiWI CGTACG 1 cut(s) 621
BslFI GGGAC 3 cut(s) 180, 539, 688
BslI CCNNNNNNNGG 7 cut(s) 191, 220, 238, 266, 289, 360, 563
BsmAI GTCTC 2 cut(s) 164, 471
BsmBI CGTCTC 1 cut(s) 164
BsmFI GGGAC 3 cut(s) 180, 539, 688
BsnI GGCC 1 cut(s) 364
BsoBI CYCGRG 1 cut(s) 355
Bsp1286I GDGCHC 1 cut(s) 322
Bsp143I GATC 5 cut(s) 106, 180, 264, 269, 592
BspACI CCGC 8 cut(s) 39, 242, 273, 284, 401, 438, 615, 773
BspANI GGCC 1 cut(s) 364
BspCNI CTCAG 1 cut(s) 505
BspFNI CGCG 1 cut(s) 440
BspLI GGNNCC 8 cut(s) 68, 108, 196, 271, 321, 365, 676, 694
BspPI GGATC 5 cut(s) 101, 114, 175, 264, 277
BspQI GCTCTTC 1 cut(s) 650
BspT107I GGYRCC 2 cut(s) 66, 319
BssECI CCNNGG 3 cut(s) 70, 354, 367
BssMI GATC 5 cut(s) 106, 180, 264, 269, 592
BssNI GRCGYC 2 cut(s) 67, 252
Bst4CI ACNGT 4 cut(s) 324, 394, 465, 572
Bst6I CTCTTC 1 cut(s) 650
BstACI GRCGYC 2 cut(s) 67, 252
BstBAI YACGTR 3 cut(s) 133, 162, 745
BstC8I GCNNGC 1 cut(s) 401
BstDEI CTNAG 2 cut(s) 287, 492
BstDSI CCRYGG 2 cut(s) 70, 367
BstENI CCTNNNNNAGG 1 cut(s) 358
BstF5I GGATG 2 cut(s) 172, 228
BstFNI CGCG 1 cut(s) 440
BstH2I RGCGCY 2 cut(s) 70, 352
BstHHI GCGC 4 cut(s) 69, 351, 442, 488
BstKTI GATC 5 cut(s) 109, 183, 267, 272, 595
BstMAI GTCTC 2 cut(s) 164, 471
BstMBI GATC 5 cut(s) 106, 180, 264, 269, 592
BstMWI GCNNNNNNNGC 3 cut(s) 521, 692, 767
BstSLI GKGCMC 1 cut(s) 322
BstUI CGCG 1 cut(s) 440
BstV1I GCAGC 3 cut(s) 558, 561, 782
BstX2I RGATCY 2 cut(s) 106, 269
BstXI CCANNNNNNTGG 1 cut(s) 133
BstYI RGATCY 2 cut(s) 106, 269
BsuRI GGCC 1 cut(s) 364
BtgI CCRYGG 2 cut(s) 70, 367
BtrI CACGTC 2 cut(s) 235, 307
BtsCI GGATG 2 cut(s) 172, 228
Cac8I GCNNGC 1 cut(s) 401
CfoI GCGC 4 cut(s) 69, 351, 442, 488
Cfr13I GGNCC 4 cut(s) 194, 295, 363, 675
CseI GACGC 2 cut(s) 181, 541
Csp6I GTAC 4 cut(s) 451, 568, 622, 628
CviQI GTAC 4 cut(s) 451, 568, 622, 628
DdeI CTNAG 2 cut(s) 287, 492
DinI GGCGCC 1 cut(s) 68
DpnI GATC 5 cut(s) 108, 182, 266, 271, 594
DpnII GATC 5 cut(s) 106, 180, 264, 269, 592
DrdI GACNNNNNNGTC 1 cut(s) 251
DseDI GACNNNNNNGTC 1 cut(s) 251
Eam1104I CTCTTC 1 cut(s) 650
EarI CTCTTC 1 cut(s) 650
EciI GGCGGA 2 cut(s) 28, 257
Eco47I GGWCC 3 cut(s) 194, 295, 675
Eco57I CTGAAG 1 cut(s) 101
Eco72I CACGTG 2 cut(s) 133, 162
Eco88I CYCGRG 1 cut(s) 355
EcoNI CCTNNNNNAGG 1 cut(s) 358
EcoO109I RGGNCCY 3 cut(s) 194, 295, 363
EgeI GGCGCC 1 cut(s) 68
EheI GGCGCC 1 cut(s) 68
Esp3I CGTCTC 1 cut(s) 164
FaiI YATR 3 cut(s) 501, 513, 606
FaqI GGGAC 3 cut(s) 180, 539, 688
FauI CCCGC 4 cut(s) 291, 408, 445, 608
Fnu4HI GCNGC 3 cut(s) 547, 550, 771
FokI GGATG 2 cut(s) 179, 215
Fsp4HI GCNGC 3 cut(s) 547, 550, 771
GlaI GCGC 4 cut(s) 68, 350, 441, 487
GluI GCNGC 3 cut(s) 547, 550, 771
HaeII RGCGCY 2 cut(s) 70, 352
HaeIII GGCC 1 cut(s) 364
HapII CCGG 3 cut(s) 9, 678, 702
HgaI GACGC 2 cut(s) 181, 541
HhaI GCGC 4 cut(s) 69, 351, 442, 488
Hin1I GRCGYC 2 cut(s) 67, 252
Hin6I GCGC 4 cut(s) 67, 349, 440, 486
HinP1I GCGC 4 cut(s) 67, 349, 440, 486
HinfI GANTC 3 cut(s) 152, 206, 598
HpaII CCGG 3 cut(s) 9, 678, 702
Hpy166II GTNNAC 4 cut(s) 136, 223, 331, 716
Hpy188I TCNGA 3 cut(s) 269, 597, 640
Hpy188III TCNNGA 3 cut(s) 178, 474, 673
Hpy8I GTNNAC 4 cut(s) 136, 223, 331, 716
Hpy99I CGWCG 4 cut(s) 152, 203, 469, 640
HpyAV CCTTC 2 cut(s) 643, 725
HpyCH4III ACNGT 4 cut(s) 324, 394, 465, 572
HpyCH4IV ACGT 7 cut(s) 57, 132, 161, 234, 252, 306, 744
HpyCH4V TGCA 3 cut(s) 461, 722, 770
HpyF10VI GCNNNNNNNGC 3 cut(s) 521, 692, 767
HpyF3I CTNAG 2 cut(s) 287, 492
HpySE526I ACGT 7 cut(s) 57, 132, 161, 234, 252, 306, 744
Hsp92I GRCGYC 2 cut(s) 67, 252
HspAI GCGC 4 cut(s) 67, 349, 440, 486
KasI GGCGCC 1 cut(s) 66
Kzo9I GATC 5 cut(s) 106, 180, 264, 269, 592
LguI GCTCTTC 1 cut(s) 650
LmnI GCTCC 3 cut(s) 128, 520, 692
LpnPI CCDG 6 cut(s) 22, 432, 534, 549, 691, 715
Lsp1109I GCAGC 3 cut(s) 558, 561, 782
LweI GCATC 1 cut(s) 757
MaeII ACGT 7 cut(s) 57, 132, 161, 234, 252, 306, 744
MaeIII GTNAC 4 cut(s) 25, 388, 407, 572
MalI GATC 5 cut(s) 108, 182, 266, 271, 594
MboI GATC 5 cut(s) 106, 180, 264, 269, 592
MboII GAAGA 2 cut(s) 27, 667
MfeI CAATTG 1 cut(s) 723
MflI RGATCY 2 cut(s) 106, 269
MhlI GDGCHC 1 cut(s) 322
MluCI AATT 4 cut(s) 30, 61, 502, 723
Mly113I GGCGCC 1 cut(s) 67
MlyI GAGTC 3 cut(s) 146, 200, 607
MmeI TCCRAC 2 cut(s) 357, 618
MslI CAYNNNNRTG 1 cut(s) 131
MspA1I CMGCKG 1 cut(s) 773
MspI CCGG 3 cut(s) 9, 678, 702
MunI CAATTG 1 cut(s) 723
MvnI CGCG 1 cut(s) 440
MwoI GCNNNNNNNGC 3 cut(s) 521, 692, 767
NarI GGCGCC 1 cut(s) 67
NdeII GATC 5 cut(s) 106, 180, 264, 269, 592
NlaIV GGNNCC 8 cut(s) 68, 108, 196, 271, 321, 365, 676, 694
NmuCI GTSAC 2 cut(s) 25, 388
OliI CACNNNNGTG 1 cut(s) 131
PciSI GCTCTTC 1 cut(s) 650
PcsI WCGNNNNNNNCGW 3 cut(s) 144, 403, 644
Pfl23II CGTACG 1 cut(s) 621
PkrI GCNGC 3 cut(s) 548, 551, 772
PleI GAGTC 3 cut(s) 146, 200, 606
PluTI GGCGCC 1 cut(s) 70
PmaCI CACGTG 2 cut(s) 133, 162
PmlI CACGTG 2 cut(s) 133, 162
PpsI GAGTC 3 cut(s) 146, 200, 606
Ppu21I YACGTR 3 cut(s) 133, 162, 745
PpuMI RGGWCCY 2 cut(s) 194, 295
Psp5II RGGWCCY 2 cut(s) 194, 295
PspCI CACGTG 2 cut(s) 133, 162
PspLI CGTACG 1 cut(s) 621
PspN4I GGNNCC 8 cut(s) 68, 108, 196, 271, 321, 365, 676, 694
PspPI GGNCC 4 cut(s) 194, 295, 363, 675
PspPPI RGGWCCY 2 cut(s) 194, 295
PsuI RGATCY 2 cut(s) 106, 269
RsaI GTAC 4 cut(s) 452, 569, 623, 629
RsaNI GTAC 4 cut(s) 451, 568, 622, 628
RseI CAYNNNNRTG 1 cut(s) 131
SapI GCTCTTC 1 cut(s) 650
SatI GCNGC 3 cut(s) 547, 550, 771
Sau3AI GATC 5 cut(s) 106, 180, 264, 269, 592
Sau96I GGNCC 4 cut(s) 194, 295, 363, 675
SchI GAGTC 3 cut(s) 146, 200, 607
SduI GDGCHC 1 cut(s) 322
SfaNI GCATC 1 cut(s) 757
SfoI GGCGCC 1 cut(s) 68
SinI GGWCC 3 cut(s) 194, 295, 675
SmiMI CAYNNNNRTG 1 cut(s) 131
SmlI CTYRAG 1 cut(s) 344
SmoI CTYRAG 1 cut(s) 344
Sse9I AATT 4 cut(s) 30, 61, 502, 723
SsiI CCGC 8 cut(s) 39, 242, 273, 284, 401, 438, 615, 773
SspDI GGCGCC 1 cut(s) 66
SspI AATATT 1 cut(s) 536
TaaI ACNGT 4 cut(s) 324, 394, 465, 572
TaiI ACGT 7 cut(s) 60, 135, 164, 237, 255, 309, 747
TaqI TCGA 5 cut(s) 179, 201, 209, 300, 473
TasI AATT 4 cut(s) 30, 61, 502, 723
TseFI GTSAC 2 cut(s) 25, 388
TseI GCWGC 3 cut(s) 546, 549, 770
Tsp45I GTSAC 2 cut(s) 25, 388
TspGWI ACGGA 2 cut(s) 87, 316
VpaK11BI GGWCC 3 cut(s) 194, 295, 675
XagI CCTNNNNNAGG 1 cut(s) 358
ZraI GACGTC 1 cut(s) 253
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.