Rh6DG031800

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0004046
Physical Location & Seq
Forward (+)
3095065 .. 3096189
1125 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG031800.1

Sequence Viewer

Length: 825 bp
ATGGCTTCCGGCTACGAAGTAGAGGTCACAATTTCTTCCGCCAAAGACCTCAAAAACGTGAATTGGCGCCACGGAGAGCTGAAGCCCTACGCTGTTGTTTGGGTGGATCCAAACAATAAATGCTCCACCCACGTGGACGAATACGGCGACGACTCGCCCACGTGGGATGAGACACTCTCGATCCCGTTACGGGGTCCCGTCGAGGACTCGACCCTTTTCGTGGACATCATCCACGTCAAGGCGGACGAGGACGTCAAGCCGTTGATCGGATCCGCAAAACTCCCGCTTAGGGAGGTTCTCGATGACGTGGGTCTCGGGGCGCCGTCCATTCACAAGTTGAAGCTCAAGCGCCCCTCGGGTAGGCCCCACGGGAAGTTGGAAGTCAAAGTCACAGTCCGCCCGCCCCGTTACCAAGCACCTGCCCCGTATCACGCTCCCGCGCCCTACGGGTACGGGGTACCACCGCCGCAGTCGAGAGACTACGGCGCACCTCAGCAGTATAATTATCAATATGCCCCTCCTGCCCAACCCCAATATTACCAAAGCAGCAGCGTCCCACAAGCAGGGTACGGTTACAATCAGCCATCGTACGATCAGAGTCAGTATGGGAGTGGCGGGTCGTACGGGTACGGGTCGTCGGAGAAGCCGAAGGAGAAGAGCAAGTTTGGGGTCGGGACCGGATTGGCTGTGGGAGCCGTAGCCGGAGTGTTGGGTGGACTTGCGATTGCTGAAGGAGTGGATTACGTGGAGGACAAGATTGCTGATGATGCAGCGGAGAAAGTGGAGGAGGATCTTGGCTATGACGATTTTGGCGATGATGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

274

Amino Acids

29.7

Weight (kDa)

4.83

Isoelectric Point (pI)

39.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 7 - 100 1.5e-10 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 427
AasI GACNNNNNNGTC 1 cut(s) 251
AatII GACGTC 1 cut(s) 255
Acc36I ACCTGC 1 cut(s) 427
Acc65I GGTACC 1 cut(s) 457
AccB1I GGYRCC 3 cut(s) 66, 319, 457
AccII CGCG 1 cut(s) 440
AclWI GGATC 6 cut(s) 101, 114, 175, 264, 277, 798
AcuI CTGAAG 2 cut(s) 101, 750
AcvI CACGTG 2 cut(s) 133, 162
AcyI GRCGYC 3 cut(s) 67, 252, 320
AfaI GTAC 6 cut(s) 452, 459, 569, 590, 623, 629
AfiI CCNNNNNNNGG 8 cut(s) 190, 191, 220, 238, 266, 289, 360, 563
AgsI TTSAA 1 cut(s) 340
AjiI CACGTC 2 cut(s) 235, 307
AleI CACNNNNGTG 1 cut(s) 131
AluBI AGCT 2 cut(s) 79, 343
AluI AGCT 2 cut(s) 79, 343
Alw26I GTCTC 3 cut(s) 164, 317, 471
AlwI GGATC 6 cut(s) 101, 114, 175, 264, 277, 798
Ama87I CYCGRG 2 cut(s) 314, 355
AoxI GGCC 1 cut(s) 362
ApeKI GCWGC 3 cut(s) 546, 549, 770
ArsI GACNNNNNNTTYG 4 cut(s) 9, 41, 378, 410
Asp718I GGTACC 1 cut(s) 457
AspLEI GCGC 5 cut(s) 69, 322, 351, 442, 488
AspS9I GGNCC 3 cut(s) 194, 363, 675
AvaI CYCGRG 2 cut(s) 314, 355
AvaII GGWCC 2 cut(s) 194, 675
BamHI GGATCC 2 cut(s) 106, 269
BanI GGYRCC 3 cut(s) 66, 319, 457
BbrPI CACGTG 2 cut(s) 133, 162
BbvCI CCTCAGC 1 cut(s) 492
BbvI GCAGC 3 cut(s) 558, 561, 782
BccI CCATC 1 cut(s) 592
BceAI ACGGC 5 cut(s) 160, 244, 307, 499, 680
BcoDI GTCTC 3 cut(s) 164, 317, 471
BfoI RGCGCY 3 cut(s) 70, 323, 352
BfuAI ACCTGC 1 cut(s) 427
BisI GCNGC 4 cut(s) 467, 547, 550, 771
BlsI GCNGC 4 cut(s) 468, 548, 551, 772
Bme18I GGWCC 2 cut(s) 194, 675
BmeT110I CYCGRG 2 cut(s) 314, 355
BmgBI CACGTC 2 cut(s) 235, 307
BmgT120I GGNCC 3 cut(s) 194, 363, 675
BmsI GCATC 1 cut(s) 757
BoxI GACNNNNGTC 1 cut(s) 309
BplI GAGNNNNNCTC 2 cut(s) 161, 193
Bpu10I CCTNAGC 2 cut(s) 287, 492
BpuEI CTTGAG 1 cut(s) 329
BsaAI YACGTR 3 cut(s) 133, 162, 745
BsaHI GRCGYC 3 cut(s) 67, 252, 320
BsaI GGTCTC 1 cut(s) 317
BsaJI CCNNGG 3 cut(s) 70, 354, 367
BsaWI WCCGGW 1 cut(s) 677
Bsc4I CCNNNNNNNGG 8 cut(s) 190, 191, 220, 238, 266, 289, 360, 563
BseDI CCNNGG 3 cut(s) 70, 354, 367
BseGI GGATG 2 cut(s) 172, 228
BseLI CCNNNNNNNGG 8 cut(s) 190, 191, 220, 238, 266, 289, 360, 563
BseMII CTCAG 1 cut(s) 506
BseRI GAGGAG 1 cut(s) 800
BseXI GCAGC 3 cut(s) 558, 561, 782
Bsh1236I CGCG 1 cut(s) 440
BshFI GGCC 1 cut(s) 364
BshNI GGYRCC 3 cut(s) 66, 319, 457
BsiHKCI CYCGRG 2 cut(s) 314, 355
BsiSI CCGG 3 cut(s) 9, 678, 702
BsiWI CGTACG 2 cut(s) 588, 621
BslFI GGGAC 3 cut(s) 180, 539, 688
BslI CCNNNNNNNGG 8 cut(s) 190, 191, 220, 238, 266, 289, 360, 563
BsmAI GTCTC 3 cut(s) 164, 317, 471
BsmFI GGGAC 3 cut(s) 180, 539, 688
BsnI GGCC 1 cut(s) 364
Bso31I GGTCTC 1 cut(s) 317
BsoBI CYCGRG 2 cut(s) 314, 355
Bsp143I GATC 6 cut(s) 106, 180, 264, 269, 592, 790
BspANI GGCC 1 cut(s) 364
BspCNI CTCAG 1 cut(s) 505
BspFNI CGCG 1 cut(s) 440
BspMI ACCTGC 1 cut(s) 427
BspPI GGATC 6 cut(s) 101, 114, 175, 264, 277, 798
BspQI GCTCTTC 1 cut(s) 650
BspT107I GGYRCC 3 cut(s) 66, 319, 457
BspTNI GGTCTC 1 cut(s) 317
BssECI CCNNGG 3 cut(s) 70, 354, 367
BssMI GATC 6 cut(s) 106, 180, 264, 269, 592, 790
BssNI GRCGYC 3 cut(s) 67, 252, 320
Bst4CI ACNGT 2 cut(s) 394, 572
Bst6I CTCTTC 1 cut(s) 650
BstACI GRCGYC 3 cut(s) 67, 252, 320
BstBAI YACGTR 3 cut(s) 133, 162, 745
BstC8I GCNNGC 1 cut(s) 401
BstDEI CTNAG 2 cut(s) 287, 492
BstDSI CCRYGG 2 cut(s) 70, 367
BstENI CCTNNNNNAGG 1 cut(s) 358
BstF5I GGATG 2 cut(s) 172, 228
BstFNI CGCG 1 cut(s) 440
BstH2I RGCGCY 3 cut(s) 70, 323, 352
BstHHI GCGC 5 cut(s) 69, 322, 351, 442, 488
BstKTI GATC 6 cut(s) 109, 183, 267, 272, 595, 793
BstMAI GTCTC 3 cut(s) 164, 317, 471
BstMBI GATC 6 cut(s) 106, 180, 264, 269, 592, 790
BstMWI GCNNNNNNNGC 3 cut(s) 521, 692, 767
BstPAI GACNNNNGTC 1 cut(s) 309
BstUI CGCG 1 cut(s) 440
BstV1I GCAGC 3 cut(s) 558, 561, 782
BstX2I RGATCY 3 cut(s) 106, 269, 790
BstXI CCANNNNNNTGG 1 cut(s) 133
BstYI RGATCY 3 cut(s) 106, 269, 790
BsuRI GGCC 1 cut(s) 364
BtgI CCRYGG 2 cut(s) 70, 367
BtrI CACGTC 2 cut(s) 235, 307
BtsCI GGATG 2 cut(s) 172, 228
BveI ACCTGC 1 cut(s) 427
Cac8I GCNNGC 1 cut(s) 401
CfoI GCGC 5 cut(s) 69, 322, 351, 442, 488
Cfr13I GGNCC 3 cut(s) 194, 363, 675
CseI GACGC 1 cut(s) 541
Csp6I GTAC 6 cut(s) 451, 458, 568, 589, 622, 628
CviQI GTAC 6 cut(s) 451, 458, 568, 589, 622, 628
DdeI CTNAG 2 cut(s) 287, 492
DinI GGCGCC 2 cut(s) 68, 321
DpnI GATC 6 cut(s) 108, 182, 266, 271, 594, 792
DpnII GATC 6 cut(s) 106, 180, 264, 269, 592, 790
DrdI GACNNNNNNGTC 1 cut(s) 251
DseDI GACNNNNNNGTC 1 cut(s) 251
Eam1104I CTCTTC 1 cut(s) 650
EarI CTCTTC 1 cut(s) 650
EciI GGCGGA 3 cut(s) 28, 257, 386
Eco31I GGTCTC 1 cut(s) 317
Eco47I GGWCC 2 cut(s) 194, 675
Eco57I CTGAAG 2 cut(s) 101, 750
Eco72I CACGTG 2 cut(s) 133, 162
Eco88I CYCGRG 2 cut(s) 314, 355
EcoNI CCTNNNNNAGG 1 cut(s) 358
EcoO109I RGGNCCY 2 cut(s) 194, 363
EgeI GGCGCC 2 cut(s) 68, 321
EheI GGCGCC 2 cut(s) 68, 321
FaiI YATR 4 cut(s) 501, 513, 606, 801
FaqI GGGAC 3 cut(s) 180, 539, 688
FauI CCCGC 4 cut(s) 291, 408, 445, 608
Fnu4HI GCNGC 4 cut(s) 467, 547, 550, 771
FokI GGATG 2 cut(s) 179, 215
Fsp4HI GCNGC 4 cut(s) 467, 547, 550, 771
GlaI GCGC 5 cut(s) 68, 321, 350, 441, 487
GluI GCNGC 4 cut(s) 467, 547, 550, 771
HaeII RGCGCY 3 cut(s) 70, 323, 352
HaeIII GGCC 1 cut(s) 364
HapII CCGG 3 cut(s) 9, 678, 702
HgaI GACGC 1 cut(s) 541
HhaI GCGC 5 cut(s) 69, 322, 351, 442, 488
Hin1I GRCGYC 3 cut(s) 67, 252, 320
Hin6I GCGC 5 cut(s) 67, 320, 349, 440, 486
HinP1I GCGC 5 cut(s) 67, 320, 349, 440, 486
HinfI GANTC 3 cut(s) 152, 206, 598
HpaII CCGG 3 cut(s) 9, 678, 702
Hpy166II GTNNAC 3 cut(s) 136, 223, 716
Hpy188I TCNGA 3 cut(s) 269, 597, 640
Hpy188III TCNNGA 4 cut(s) 178, 299, 474, 673
Hpy8I GTNNAC 3 cut(s) 136, 223, 716
Hpy99I CGWCG 3 cut(s) 152, 203, 640
HpyAV CCTTC 2 cut(s) 643, 725
HpyCH4III ACNGT 2 cut(s) 394, 572
HpyCH4IV ACGT 7 cut(s) 57, 132, 161, 234, 252, 306, 744
HpyCH4V TGCA 1 cut(s) 770
HpyF10VI GCNNNNNNNGC 3 cut(s) 521, 692, 767
HpyF3I CTNAG 2 cut(s) 287, 492
HpySE526I ACGT 7 cut(s) 57, 132, 161, 234, 252, 306, 744
Hsp92I GRCGYC 3 cut(s) 67, 252, 320
HspAI GCGC 5 cut(s) 67, 320, 349, 440, 486
KasI GGCGCC 2 cut(s) 66, 319
KflI GGGWCCC 1 cut(s) 194
KpnI GGTACC 1 cut(s) 461
Kzo9I GATC 6 cut(s) 106, 180, 264, 269, 592, 790
LguI GCTCTTC 1 cut(s) 650
LmnI GCTCC 3 cut(s) 128, 439, 692
LpnPI CCDG 6 cut(s) 22, 432, 534, 549, 691, 715
Lsp1109I GCAGC 3 cut(s) 558, 561, 782
LweI GCATC 1 cut(s) 757
MaeII ACGT 7 cut(s) 57, 132, 161, 234, 252, 306, 744
MaeIII GTNAC 5 cut(s) 25, 186, 388, 407, 572
MalI GATC 6 cut(s) 108, 182, 266, 271, 594, 792
MboI GATC 6 cut(s) 106, 180, 264, 269, 592, 790
MboII GAAGA 2 cut(s) 27, 667
MflI RGATCY 3 cut(s) 106, 269, 790
MluCI AATT 3 cut(s) 30, 61, 502
Mly113I GGCGCC 2 cut(s) 67, 320
MlyI GAGTC 3 cut(s) 146, 200, 607
MmeI TCCRAC 2 cut(s) 357, 618
MslI CAYNNNNRTG 1 cut(s) 131
MspA1I CMGCKG 1 cut(s) 773
MspI CCGG 3 cut(s) 9, 678, 702
MvnI CGCG 1 cut(s) 440
MwoI GCNNNNNNNGC 3 cut(s) 521, 692, 767
NarI GGCGCC 2 cut(s) 67, 320
NdeII GATC 6 cut(s) 106, 180, 264, 269, 592, 790
NmuCI GTSAC 2 cut(s) 25, 388
OliI CACNNNNGTG 1 cut(s) 131
PaqCI CACCTGC 1 cut(s) 427
PciSI GCTCTTC 1 cut(s) 650
PcsI WCGNNNNNNNCGW 3 cut(s) 144, 644, 810
Pfl23II CGTACG 2 cut(s) 588, 621
PkrI GCNGC 4 cut(s) 468, 548, 551, 772
PleI GAGTC 3 cut(s) 146, 200, 606
PluTI GGCGCC 2 cut(s) 70, 323
PmaCI CACGTG 2 cut(s) 133, 162
PmlI CACGTG 2 cut(s) 133, 162
PpsI GAGTC 3 cut(s) 146, 200, 606
Ppu21I YACGTR 3 cut(s) 133, 162, 745
PpuMI RGGWCCY 1 cut(s) 194
PshAI GACNNNNGTC 1 cut(s) 309
Psp5II RGGWCCY 1 cut(s) 194
PspCI CACGTG 2 cut(s) 133, 162
PspLI CGTACG 2 cut(s) 588, 621
PspPI GGNCC 3 cut(s) 194, 363, 675
PspPPI RGGWCCY 1 cut(s) 194
PsuI RGATCY 3 cut(s) 106, 269, 790
RsaI GTAC 6 cut(s) 452, 459, 569, 590, 623, 629
RsaNI GTAC 6 cut(s) 451, 458, 568, 589, 622, 628
RseI CAYNNNNRTG 1 cut(s) 131
SapI GCTCTTC 1 cut(s) 650
SatI GCNGC 4 cut(s) 467, 547, 550, 771
Sau3AI GATC 6 cut(s) 106, 180, 264, 269, 592, 790
Sau96I GGNCC 3 cut(s) 194, 363, 675
SchI GAGTC 3 cut(s) 146, 200, 607
SfaNI GCATC 1 cut(s) 757
SfoI GGCGCC 2 cut(s) 68, 321
SinI GGWCC 2 cut(s) 194, 675
SmiMI CAYNNNNRTG 1 cut(s) 131
SmlI CTYRAG 1 cut(s) 344
SmoI CTYRAG 1 cut(s) 344
Sse9I AATT 3 cut(s) 30, 61, 502
SspDI GGCGCC 2 cut(s) 66, 319
SspI AATATT 1 cut(s) 536
TaaI ACNGT 2 cut(s) 394, 572
TaiI ACGT 7 cut(s) 60, 135, 164, 237, 255, 309, 747
TaqI TCGA 5 cut(s) 179, 201, 209, 300, 473
TasI AATT 3 cut(s) 30, 61, 502
TauI GCSGC 1 cut(s) 469
TseFI GTSAC 2 cut(s) 25, 388
TseI GCWGC 3 cut(s) 546, 549, 770
Tsp45I GTSAC 2 cut(s) 25, 388
TspGWI ACGGA 1 cut(s) 87
VpaK11BI GGWCC 2 cut(s) 194, 675
XagI CCTNNNNNAGG 1 cut(s) 358
ZraI GACGTC 1 cut(s) 253
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.