FvH4_2g02750

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0004046
Physical Location & Seq
Forward (+)
2179547 .. 2181250
1704 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g02750.t2

Sequence Viewer

Length: 819 bp
ATGGCTTCCGGCTACGAAGTAGAGCTCACGATTTCTTCCGCCAAAGACCTCAAAAACGTGAATTGGCGCCACGGAGAGCTGAAACCCTACGCCGTGGTTTGGGTGGATCCAAACAACAAATGCTCTACCCACGTGGACGAATACGGTGACGACTCGCCCACGTGGGATGAGACGCTCTCAATCCCGCTACGGGGCCCGGTCGAGGACTCGACCCTCTTCGTGGACGTTATCCATGTTAAGGCGGACGAGGACGTTAAGCCTTTGATCGGGTCCGCGAAACTCCCACTTAGGGAGGTCCTCGATGACGTGGGGCTCGGTGCGCCGTCCGTTCATAAGTTGAAACTCAAGCGCCCCTCGGGTAGGCCTCAAGGAAAATTAGAAGTCAAAGTCACGATCCGCCCGCCCCGATACCAAGCACCCGACCCATATCACGCCCCCGCGCCATACGGGTACGGGGTCCCTCCACCGCAGTCGAGAGACTACGGCGCACCTCAGCAGTACAATTACCCATATGCCCCTTCCGCCCAACCCCAATACTACCAAAGCGTCCCACAAGCAGGGTACGGTTACAATCAGCAGTCTTACGATCAGAGTCAGTATGGGAGTGGCGGGTCGTACGGGTACGGGTCTTCGGAGAAGCCGAAGGAGAAGAGCAAGTTTGGGGTCGGGACCGGAGTGGCTGTTGGCGCTGTAGCCGGAGTTTTGGGTGGACTTGCGATTGCTGAAGGAGTGGATTACTTGGAGGACAAGGTAGCTGATGACGCGGCGGAGAAAGTGGAGGAGGATCTCGGCTATGACGATTTCGGTGATGATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

273

Amino Acids

29.53

Weight (kDa)

4.73

Isoelectric Point (pI)

38.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 7 - 100 1.4e-10 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 66
AccII CGCG 3 cut(s) 275, 440, 764
AclWI GGATC 4 cut(s) 101, 114, 388, 792
AcuI CTGAAG 1 cut(s) 744
AcvI CACGTG 2 cut(s) 133, 162
AcyI GRCGYC 1 cut(s) 67
AfaI GTAC 5 cut(s) 452, 500, 563, 617, 623
AgsI TTSAA 1 cut(s) 340
AjiI CACGTC 1 cut(s) 307
AluBI AGCT 3 cut(s) 25, 79, 755
AluI AGCT 3 cut(s) 25, 79, 755
Alw21I GWGCWC 1 cut(s) 27
Alw26I GTCTC 2 cut(s) 164, 471
AlwI GGATC 4 cut(s) 101, 114, 388, 792
Ama87I CYCGRG 1 cut(s) 355
AoxI GGCC 2 cut(s) 193, 362
ApaI GGGCCC 1 cut(s) 197
AspLEI GCGC 6 cut(s) 69, 322, 351, 442, 488, 689
AspS9I GGNCC 6 cut(s) 193, 194, 270, 295, 457, 669
AsuC2I CCSGG 1 cut(s) 197
AsuHPI GGTGA 2 cut(s) 158, 818
AvaI CYCGRG 1 cut(s) 355
AvaII GGWCC 4 cut(s) 270, 295, 457, 669
BaeGI GKGCMC 1 cut(s) 197
BamHI GGATCC 1 cut(s) 106
BanI GGYRCC 1 cut(s) 66
BanII GRGCYC 3 cut(s) 27, 197, 315
BbrPI CACGTG 2 cut(s) 133, 162
BbsI GAAGAC 1 cut(s) 621
Bbv12I GWGCWC 1 cut(s) 27
BbvCI CCTCAGC 1 cut(s) 492
BceAI ACGGC 3 cut(s) 77, 307, 499
BcnI CCSGG 1 cut(s) 197
BcoDI GTCTC 2 cut(s) 164, 471
BfmI CTRYAG 1 cut(s) 690
BfoI RGCGCY 3 cut(s) 70, 352, 690
BisI GCNGC 1 cut(s) 765
BlsI GCNGC 1 cut(s) 766
Bme1390I CCNGG 1 cut(s) 197
Bme18I GGWCC 4 cut(s) 270, 295, 457, 669
BmeT110I CYCGRG 1 cut(s) 355
BmgBI CACGTC 1 cut(s) 307
BmgT120I GGNCC 6 cut(s) 193, 194, 270, 295, 457, 669
BmiI GGNNCC 8 cut(s) 68, 108, 194, 195, 271, 458, 459, 670
BmrFI CCNGG 1 cut(s) 197
BpiI GAAGAC 1 cut(s) 621
BplI GAGNNNNNCTC 2 cut(s) 161, 193
Bpu10I CCTNAGC 1 cut(s) 492
BpuEI CTTGAG 2 cut(s) 329, 351
BpuMI CCSGG 1 cut(s) 197
BsaAI YACGTR 2 cut(s) 133, 162
BsaHI GRCGYC 1 cut(s) 67
BsaJI CCNNGG 3 cut(s) 70, 93, 354
BsaWI WCCGGW 1 cut(s) 671
BsaXI ACNNNNNCTCC 2 cut(s) 666, 696
BseDI CCNNGG 3 cut(s) 70, 93, 354
BseGI GGATG 1 cut(s) 172
BseMII CTCAG 1 cut(s) 506
BseRI GAGGAG 1 cut(s) 794
BseSI GKGCMC 1 cut(s) 197
Bsh1236I CGCG 3 cut(s) 275, 440, 764
Bsh1285I CGRYCG 1 cut(s) 201
BshFI GGCC 2 cut(s) 195, 364
BshNI GGYRCC 1 cut(s) 66
BsiEI CGRYCG 1 cut(s) 201
BsiHKAI GWGCWC 1 cut(s) 27
BsiHKCI CYCGRG 1 cut(s) 355
BsiSI CCGG 4 cut(s) 9, 197, 672, 696
BsiWI CGTACG 1 cut(s) 615
BslFI GGGAC 3 cut(s) 443, 533, 682
BsmAI GTCTC 2 cut(s) 164, 471
BsmBI CGTCTC 1 cut(s) 164
BsmFI GGGAC 3 cut(s) 443, 533, 682
BsnI GGCC 2 cut(s) 195, 364
BsoBI CYCGRG 1 cut(s) 355
Bsp120I GGGCCC 1 cut(s) 193
Bsp1286I GDGCHC 3 cut(s) 27, 197, 315
Bsp143I GATC 5 cut(s) 106, 264, 393, 586, 784
BspANI GGCC 2 cut(s) 195, 364
BspCNI CTCAG 1 cut(s) 505
BspFNI CGCG 3 cut(s) 275, 440, 764
BspLI GGNNCC 8 cut(s) 68, 108, 194, 195, 271, 458, 459, 670
BspPI GGATC 4 cut(s) 101, 114, 388, 792
BspQI GCTCTTC 1 cut(s) 644
BspT107I GGYRCC 1 cut(s) 66
BssECI CCNNGG 3 cut(s) 70, 93, 354
BssMI GATC 5 cut(s) 106, 264, 393, 586, 784
BssNI GRCGYC 1 cut(s) 67
Bst4CI ACNGT 2 cut(s) 146, 566
Bst6I CTCTTC 2 cut(s) 221, 644
BstACI GRCGYC 1 cut(s) 67
BstBAI YACGTR 2 cut(s) 133, 162
BstC8I GCNNGC 1 cut(s) 401
BstDEI CTNAG 2 cut(s) 287, 492
BstDSI CCRYGG 2 cut(s) 70, 93
BstENI CCTNNNNNAGG 1 cut(s) 358
BstF5I GGATG 1 cut(s) 172
BstFNI CGCG 3 cut(s) 275, 440, 764
BstH2I RGCGCY 3 cut(s) 70, 352, 690
BstHHI GCGC 6 cut(s) 69, 322, 351, 442, 488, 689
BstKTI GATC 5 cut(s) 109, 267, 396, 589, 787
BstMAI GTCTC 2 cut(s) 164, 471
BstMBI GATC 5 cut(s) 106, 264, 393, 586, 784
BstMCI CGRYCG 1 cut(s) 201
BstMWI GCNNNNNNNGC 4 cut(s) 319, 521, 686, 761
BstSCI CCNGG 1 cut(s) 195
BstSFI CTRYAG 1 cut(s) 690
BstSLI GKGCMC 1 cut(s) 197
BstUI CGCG 3 cut(s) 275, 440, 764
BstV2I GAAGAC 1 cut(s) 621
BstX2I RGATCY 2 cut(s) 106, 784
BstYI RGATCY 2 cut(s) 106, 784
BsuRI GGCC 2 cut(s) 195, 364
BtgI CCRYGG 2 cut(s) 70, 93
BtrI CACGTC 1 cut(s) 307
BtsCI GGATG 1 cut(s) 172
Cac8I GCNNGC 1 cut(s) 401
CfoI GCGC 6 cut(s) 69, 322, 351, 442, 488, 689
Cfr13I GGNCC 6 cut(s) 193, 194, 270, 295, 457, 669
CseI GACGC 3 cut(s) 181, 535, 770
Csp6I GTAC 5 cut(s) 451, 499, 562, 616, 622
CviAII CATG 1 cut(s) 233
CviQI GTAC 5 cut(s) 451, 499, 562, 616, 622
DdeI CTNAG 2 cut(s) 287, 492
DinI GGCGCC 1 cut(s) 68
DpnI GATC 5 cut(s) 108, 266, 395, 588, 786
DpnII GATC 5 cut(s) 106, 264, 393, 586, 784
Eam1104I CTCTTC 2 cut(s) 221, 644
EarI CTCTTC 2 cut(s) 221, 644
EciI GGCGGA 5 cut(s) 28, 257, 386, 511, 782
Ecl136II GAGCTC 1 cut(s) 25
Eco147I AGGCCT 1 cut(s) 364
Eco24I GRGCYC 3 cut(s) 27, 197, 315
Eco47I GGWCC 4 cut(s) 270, 295, 457, 669
Eco53kI GAGCTC 1 cut(s) 25
Eco57I CTGAAG 1 cut(s) 744
Eco72I CACGTG 2 cut(s) 133, 162
Eco88I CYCGRG 1 cut(s) 355
EcoICRI GAGCTC 1 cut(s) 25
EcoNI CCTNNNNNAGG 1 cut(s) 358
EcoO109I RGGNCCY 3 cut(s) 193, 295, 457
EcoT38I GRGCYC 3 cut(s) 27, 197, 315
EgeI GGCGCC 1 cut(s) 68
EheI GGCGCC 1 cut(s) 68
Esp3I CGTCTC 1 cut(s) 164
FaeI CATG 1 cut(s) 236
FaiI YATR 8 cut(s) 234, 333, 427, 445, 511, 513, 600, 795
FaqI GGGAC 3 cut(s) 443, 533, 682
FatI CATG 1 cut(s) 232
FauI CCCGC 4 cut(s) 192, 408, 445, 602
FauNDI CATATG 1 cut(s) 511
Fnu4HI GCNGC 1 cut(s) 765
FokI GGATG 1 cut(s) 179
FriOI GRGCYC 3 cut(s) 27, 197, 315
Fsp4HI GCNGC 1 cut(s) 765
GlaI GCGC 6 cut(s) 68, 321, 350, 441, 487, 688
GluI GCNGC 1 cut(s) 765
HaeII RGCGCY 3 cut(s) 70, 352, 690
HaeIII GGCC 2 cut(s) 195, 364
HapII CCGG 4 cut(s) 9, 197, 672, 696
HgaI GACGC 3 cut(s) 181, 535, 770
HhaI GCGC 6 cut(s) 69, 322, 351, 442, 488, 689
Hin1I GRCGYC 1 cut(s) 67
Hin1II CATG 1 cut(s) 236
Hin6I GCGC 6 cut(s) 67, 320, 349, 440, 486, 687
HinP1I GCGC 6 cut(s) 67, 320, 349, 440, 486, 687
HinfI GANTC 3 cut(s) 152, 206, 592
HpaII CCGG 4 cut(s) 9, 197, 672, 696
HphI GGTGA 2 cut(s) 158, 818
Hpy166II GTNNAC 3 cut(s) 136, 223, 710
Hpy188I TCNGA 2 cut(s) 591, 634
Hpy188III TCNNGA 4 cut(s) 28, 391, 474, 667
Hpy8I GTNNAC 3 cut(s) 136, 223, 710
HpyAV CCTTC 3 cut(s) 528, 637, 719
HpyCH4III ACNGT 2 cut(s) 146, 566
HpyCH4IV ACGT 6 cut(s) 57, 132, 161, 225, 252, 306
HpyF10VI GCNNNNNNNGC 4 cut(s) 319, 521, 686, 761
HpyF3I CTNAG 2 cut(s) 287, 492
HpySE526I ACGT 6 cut(s) 57, 132, 161, 225, 252, 306
Hsp92I GRCGYC 1 cut(s) 67
Hsp92II CATG 1 cut(s) 236
HspAI GCGC 6 cut(s) 67, 320, 349, 440, 486, 687
KasI GGCGCC 1 cut(s) 66
KflI GGGWCCC 1 cut(s) 457
Kzo9I GATC 5 cut(s) 106, 264, 393, 586, 784
LguI GCTCTTC 1 cut(s) 644
LpnPI CCDG 5 cut(s) 22, 210, 543, 685, 709
MaeII ACGT 6 cut(s) 57, 132, 161, 225, 252, 306
MaeIII GTNAC 3 cut(s) 146, 388, 566
MalI GATC 5 cut(s) 108, 266, 395, 588, 786
MboI GATC 5 cut(s) 106, 264, 393, 586, 784
MboII GAAGA 4 cut(s) 27, 208, 621, 661
MflI RGATCY 2 cut(s) 106, 784
MhlI GDGCHC 3 cut(s) 27, 197, 315
MluCI AATT 3 cut(s) 61, 374, 502
Mly113I GGCGCC 1 cut(s) 67
MlyI GAGTC 3 cut(s) 146, 200, 601
MseI TTAA 2 cut(s) 237, 255
MspI CCGG 4 cut(s) 9, 197, 672, 696
MspR9I CCNGG 1 cut(s) 197
MvnI CGCG 3 cut(s) 275, 440, 764
MwoI GCNNNNNNNGC 4 cut(s) 319, 521, 686, 761
NarI GGCGCC 1 cut(s) 67
NciI CCSGG 1 cut(s) 197
NdeI CATATG 1 cut(s) 511
NdeII GATC 5 cut(s) 106, 264, 393, 586, 784
NlaIII CATG 1 cut(s) 236
NlaIV GGNNCC 8 cut(s) 68, 108, 194, 195, 271, 458, 459, 670
NmeAIII GCCGAG 1 cut(s) 768
NmuCI GTSAC 2 cut(s) 146, 388
PceI AGGCCT 1 cut(s) 364
PciSI GCTCTTC 1 cut(s) 644
PcsI WCGNNNNNNNCGW 2 cut(s) 638, 795
Pfl23II CGTACG 1 cut(s) 615
PkrI GCNGC 1 cut(s) 766
PleI GAGTC 3 cut(s) 146, 200, 600
PluTI GGCGCC 1 cut(s) 70
PmaCI CACGTG 2 cut(s) 133, 162
PmlI CACGTG 2 cut(s) 133, 162
PpsI GAGTC 3 cut(s) 146, 200, 600
Ppu21I YACGTR 2 cut(s) 133, 162
PpuMI RGGWCCY 2 cut(s) 295, 457
Psp124BI GAGCTC 1 cut(s) 27
Psp5II RGGWCCY 2 cut(s) 295, 457
PspCI CACGTG 2 cut(s) 133, 162
PspLI CGTACG 1 cut(s) 615
PspN4I GGNNCC 8 cut(s) 68, 108, 194, 195, 271, 458, 459, 670
PspOMI GGGCCC 1 cut(s) 193
PspPI GGNCC 6 cut(s) 193, 194, 270, 295, 457, 669
PspPPI RGGWCCY 2 cut(s) 295, 457
PsuI RGATCY 2 cut(s) 106, 784
RsaI GTAC 5 cut(s) 452, 500, 563, 617, 623
RsaNI GTAC 5 cut(s) 451, 499, 562, 616, 622
SacI GAGCTC 1 cut(s) 27
SapI GCTCTTC 1 cut(s) 644
SaqAI TTAA 2 cut(s) 237, 255
SatI GCNGC 1 cut(s) 765
Sau3AI GATC 5 cut(s) 106, 264, 393, 586, 784
Sau96I GGNCC 6 cut(s) 193, 194, 270, 295, 457, 669
SchI GAGTC 3 cut(s) 146, 200, 601
ScrFI CCNGG 1 cut(s) 197
SduI GDGCHC 3 cut(s) 27, 197, 315
SfcI CTRYAG 1 cut(s) 690
SfoI GGCGCC 1 cut(s) 68
SinI GGWCC 4 cut(s) 270, 295, 457, 669
SmlI CTYRAG 2 cut(s) 344, 366
SmoI CTYRAG 2 cut(s) 344, 366
Sse9I AATT 3 cut(s) 61, 374, 502
SseBI AGGCCT 1 cut(s) 364
SspDI GGCGCC 1 cut(s) 66
SstI GAGCTC 1 cut(s) 27
StuI AGGCCT 1 cut(s) 364
StyD4I CCNGG 1 cut(s) 195
TaaI ACNGT 2 cut(s) 146, 566
TaiI ACGT 6 cut(s) 60, 135, 164, 228, 255, 309
TaqI TCGA 4 cut(s) 201, 209, 300, 473
TasI AATT 3 cut(s) 61, 374, 502
TatI WGTACW 1 cut(s) 498
TauI GCSGC 1 cut(s) 767
Tru1I TTAA 2 cut(s) 237, 255
Tru9I TTAA 2 cut(s) 237, 255
TseFI GTSAC 2 cut(s) 146, 388
Tsp45I GTSAC 2 cut(s) 146, 388
TspDTI ATGAA 1 cut(s) 320
TspGWI ACGGA 2 cut(s) 87, 316
VpaK11BI GGWCC 4 cut(s) 270, 295, 457, 669
XagI CCTNNNNNAGG 1 cut(s) 358
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.