Rroxscaffold_2G00106560

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0004046
Physical Location & Seq
Forward (+)
30138792 .. 30139625
834 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00106560.1

Sequence Viewer

Length: 834 bp
ATGGCGTCAGGCCACGAAGTCCAACTGAAGCTCTCCTCCGCCAGAGACCTCAAGAACGTGAACTGGAGACACGGCCCCGTCAAGCCGTACGCCGTCGGGTGGGTCGAGCCGAGGAACAAGCTCTCCACCAAGGTGGACGAGGATGGCGACACGTCGCCCGAATGGGACGAGACGCTGACAATCCCTATCCCCAGTCCTGTCAACGACGACACCATGCTCTACATCGACATCGTGCATGCCGGAGCTGAGCCCGACACCAAACAGCTGGTGGGATCTGCTCGGCTCCGGCTCCGCGACGTTGTCGATGACGTCGGATATGAAGAACGCGCCAGCCGCACTCTTCACCTCAAAAGGCCCTCCGGTAGGCCACAGGGAAAGGTGGACGTCAAGGTTACGGTGAGGGAGCCGCGCTATCGTGCGCCGGAGCAGTATTATGCGCCGCCTTTTGAGGTCCCACCGGCAGGCTCAGGAGTTTACCATGCTCCACCACCGCCGCCACCTTATGGCAACCCATACGCCACACCACCACCGCCTCCGCCAGTGTATGGTGGAACGTACGCTGCACCGCCTTCCGGGTATCCCTACAACGCGACTCCGGTGGCGTACAGTCAGCCAGCTCAGCCAAGTTATGGTCAACTGCAGTACGGGCAGGAGGAGAAGAAGAAGAGCAAGTTTGGTGGGATGGGAACGGGATTGGCTGTGGGGGCGGTAGCGGGGGCGCTGGGTGCATTTGCGTTGGCTGAGGGTGCTGATTATGTGGAGGACAAAATTGCAGACGACGTGTCGGAGAAGGTGGTGGATAATTTGAGCTACTATGATGGCGATGACTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

277

Amino Acids

30.02

Weight (kDa)

5.11

Isoelectric Point (pI)

42.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 6 - 104 3.5e-10 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 312, 387
AccB7I CCANNNNNTGG 3 cut(s) 503, 545, 629
AccII CGCG 4 cut(s) 294, 327, 409, 590
AclWI GGATC 1 cut(s) 280
AcuI CTGAAG 1 cut(s) 47
AcyI GRCGYC 3 cut(s) 5, 309, 384
AfaI GTAC 4 cut(s) 89, 557, 605, 644
AfiI CCNNNNNNNGG 7 cut(s) 99, 363, 461, 503, 545, 572, 629
AflIII ACRYGT 2 cut(s) 150, 780
AhdI GACNNNNNGTC 1 cut(s) 781
AjiI CACGTC 2 cut(s) 153, 781
AleI CACNNNNGTG 1 cut(s) 131
AloI GAACNNNNNNTCC 2 cut(s) 107, 139
AluBI AGCT 6 cut(s) 31, 121, 245, 265, 617, 810
AluI AGCT 6 cut(s) 31, 121, 245, 265, 617, 810
Alw26I GTCTC 3 cut(s) 39, 61, 164
AlwI GGATC 1 cut(s) 280
AoxI GGCC 4 cut(s) 10, 73, 353, 365
ApeKI GCWGC 1 cut(s) 560
AspLEI GCGC 5 cut(s) 329, 411, 421, 439, 721
AspS9I GGNCC 3 cut(s) 74, 354, 451
AsuC2I CCSGG 1 cut(s) 574
AsuHPI GGTGA 2 cut(s) 335, 409
AvaII GGWCC 1 cut(s) 451
BanII GRGCYC 1 cut(s) 252
BbvCI CCTCAGC 1 cut(s) 741
BbvI GCAGC 1 cut(s) 547
BccI CCATC 3 cut(s) 137, 676, 812
BceAI ACGGC 3 cut(s) 70, 77, 88
BciVI GTATCC 1 cut(s) 588
BcnI CCSGG 1 cut(s) 574
BcoDI GTCTC 3 cut(s) 39, 61, 164
BfmI CTRYAG 1 cut(s) 638
BfoI RGCGCY 1 cut(s) 722
BfuI GTATCC 1 cut(s) 588
BisI GCNGC 5 cut(s) 334, 407, 440, 494, 561
BlpI GCTNAGC 2 cut(s) 246, 618
BlsI GCNGC 5 cut(s) 335, 408, 441, 495, 562
Bme1390I CCNGG 1 cut(s) 574
Bme18I GGWCC 1 cut(s) 451
BmeRI GACNNNNNGTC 1 cut(s) 781
BmgBI CACGTC 2 cut(s) 153, 781
BmgT120I GGNCC 3 cut(s) 74, 354, 451
BmiI GGNNCC 5 cut(s) 76, 284, 290, 405, 453
BmrFI CCNGG 1 cut(s) 574
BmrI ACTGGG 1 cut(s) 186
BmuI ACTGGG 1 cut(s) 186
BpmI CTGGAG 1 cut(s) 85
Bpu10I CCTNAGC 2 cut(s) 466, 741
Bpu1102I GCTNAGC 2 cut(s) 246, 618
BpuEI CTTGAG 1 cut(s) 35
BpuMI CCSGG 1 cut(s) 574
BsaHI GRCGYC 3 cut(s) 5, 309, 384
BsaI GGTCTC 1 cut(s) 39
BsaJI CCNNGG 2 cut(s) 110, 129
BsaWI WCCGGW 2 cut(s) 359, 595
BsaXI ACNNNNNCTCC 2 cut(s) 107, 137
Bsc4I CCNNNNNNNGG 7 cut(s) 99, 363, 461, 503, 545, 572, 629
Bse118I RCCGGY 1 cut(s) 457
Bse1I ACTGG 3 cut(s) 68, 192, 539
BseDI CCNNGG 2 cut(s) 110, 129
BseGI GGATG 2 cut(s) 148, 687
BseLI CCNNNNNNNGG 7 cut(s) 99, 363, 461, 503, 545, 572, 629
BseMII CTCAG 4 cut(s) 237, 480, 632, 732
BseNI ACTGG 3 cut(s) 68, 192, 539
BseRI GAGGAG 2 cut(s) 25, 668
BseXI GCAGC 1 cut(s) 547
BseYI CCCAGC 1 cut(s) 721
BsgI GTGCAG 1 cut(s) 546
Bsh1236I CGCG 4 cut(s) 294, 327, 409, 590
BshFI GGCC 4 cut(s) 12, 75, 355, 367
BsiSI CCGG 7 cut(s) 240, 286, 360, 422, 458, 573, 596
BsiWI CGTACG 2 cut(s) 87, 555
BslFI GGGAC 2 cut(s) 179, 437
BslI CCNNNNNNNGG 7 cut(s) 99, 363, 461, 503, 545, 572, 629
BsmAI GTCTC 3 cut(s) 39, 61, 164
BsmBI CGTCTC 1 cut(s) 164
BsmFI GGGAC 2 cut(s) 179, 437
BsnI GGCC 4 cut(s) 12, 75, 355, 367
Bso31I GGTCTC 1 cut(s) 39
Bsp1286I GDGCHC 1 cut(s) 252
Bsp143I GATC 1 cut(s) 272
Bsp1720I GCTNAGC 2 cut(s) 246, 618
BspANI GGCC 4 cut(s) 12, 75, 355, 367
BspCNI CTCAG 4 cut(s) 238, 479, 631, 733
BspFNI CGCG 4 cut(s) 294, 327, 409, 590
BspLI GGNNCC 5 cut(s) 76, 284, 290, 405, 453
BspMAI CTGCAG 1 cut(s) 642
BspPI GGATC 1 cut(s) 280
BspQI GCTCTTC 1 cut(s) 659
BspTNI GGTCTC 1 cut(s) 39
BsrFI RCCGGY 1 cut(s) 457
BsrI ACTGG 3 cut(s) 68, 192, 539
BssAI RCCGGY 1 cut(s) 457
BssECI CCNNGG 2 cut(s) 110, 129
BssMI GATC 1 cut(s) 272
BssNI GRCGYC 3 cut(s) 5, 309, 384
BssT1I CCWWGG 1 cut(s) 129
Bst4CI ACNGT 2 cut(s) 397, 608
Bst6I CTCTTC 2 cut(s) 345, 659
BstACI GRCGYC 3 cut(s) 5, 309, 384
BstC8I GCNNGC 4 cut(s) 237, 331, 463, 615
BstDEI CTNAG 4 cut(s) 246, 466, 618, 741
BstENI CCTNNNNNAGG 1 cut(s) 361
BstF5I GGATG 2 cut(s) 148, 687
BstFNI CGCG 4 cut(s) 294, 327, 409, 590
BstH2I RGCGCY 1 cut(s) 722
BstHHI GCGC 5 cut(s) 329, 411, 421, 439, 721
BstKTI GATC 1 cut(s) 275
BstMAI GTCTC 3 cut(s) 39, 61, 164
BstMBI GATC 1 cut(s) 272
BstMWI GCNNNNNNNGC 6 cut(s) 333, 619, 646, 704, 725, 746
BstNSI RCATGY 1 cut(s) 239
BstSCI CCNGG 1 cut(s) 572
BstSFI CTRYAG 1 cut(s) 638
BstUI CGCG 4 cut(s) 294, 327, 409, 590
BstV1I GCAGC 1 cut(s) 547
BstX2I RGATCY 1 cut(s) 272
BstXI CCANNNNNNTGG 2 cut(s) 133, 265
BstYI RGATCY 1 cut(s) 272
BsuI GTATCC 1 cut(s) 588
BsuRI GGCC 4 cut(s) 12, 75, 355, 367
BtrI CACGTC 2 cut(s) 153, 781
BtsCI GGATG 2 cut(s) 148, 687
BtsIMutI CAGTG 1 cut(s) 546
Cac8I GCNNGC 4 cut(s) 237, 331, 463, 615
CfoI GCGC 5 cut(s) 329, 411, 421, 439, 721
Cfr10I RCCGGY 1 cut(s) 457
Cfr13I GGNCC 3 cut(s) 74, 354, 451
CseI GACGC 1 cut(s) 181
Csp6I GTAC 4 cut(s) 88, 556, 604, 643
CspCI CAANNNNNGTGG 2 cut(s) 658, 693
CviAII CATG 3 cut(s) 214, 236, 479
CviQI GTAC 4 cut(s) 88, 556, 604, 643
DdeI CTNAG 4 cut(s) 246, 466, 618, 741
DpnI GATC 1 cut(s) 274
DpnII GATC 1 cut(s) 272
DriI GACNNNNNGTC 1 cut(s) 781
Eam1104I CTCTTC 2 cut(s) 345, 659
Eam1105I GACNNNNNGTC 1 cut(s) 781
EarI CTCTTC 2 cut(s) 345, 659
EciI GGCGGA 2 cut(s) 28, 525
Eco130I CCWWGG 1 cut(s) 129
Eco24I GRGCYC 1 cut(s) 252
Eco31I GGTCTC 1 cut(s) 39
Eco47I GGWCC 1 cut(s) 451
Eco57I CTGAAG 1 cut(s) 47
EcoNI CCTNNNNNAGG 1 cut(s) 361
EcoO109I RGGNCCY 2 cut(s) 354, 451
EcoT14I CCWWGG 1 cut(s) 129
EcoT38I GRGCYC 1 cut(s) 252
ErhI CCWWGG 1 cut(s) 129
Esp3I CGTCTC 1 cut(s) 164
FaeI CATG 3 cut(s) 217, 239, 482
FaqI GGGAC 2 cut(s) 179, 437
FatI CATG 3 cut(s) 213, 235, 478
FauI CCCGC 1 cut(s) 706
Fnu4HI GCNGC 5 cut(s) 334, 407, 440, 494, 561
FokI GGATG 2 cut(s) 155, 694
FriOI GRGCYC 1 cut(s) 252
Fsp4HI GCNGC 5 cut(s) 334, 407, 440, 494, 561
GlaI GCGC 5 cut(s) 328, 410, 420, 438, 720
GluI GCNGC 5 cut(s) 334, 407, 440, 494, 561
GsaI CCCAGC 1 cut(s) 725
GsuI CTGGAG 1 cut(s) 85
HaeII RGCGCY 1 cut(s) 722
HaeIII GGCC 4 cut(s) 12, 75, 355, 367
HapII CCGG 7 cut(s) 240, 286, 360, 422, 458, 573, 596
HgaI GACGC 1 cut(s) 181
HhaI GCGC 5 cut(s) 329, 411, 421, 439, 721
Hin1I GRCGYC 3 cut(s) 5, 309, 384
Hin1II CATG 3 cut(s) 217, 239, 482
Hin6I GCGC 5 cut(s) 327, 409, 419, 437, 719
HinP1I GCGC 5 cut(s) 327, 409, 419, 437, 719
HincII GTYRAC 2 cut(s) 202, 635
HindII GTYRAC 2 cut(s) 202, 635
HinfI GANTC 1 cut(s) 592
HpaII CCGG 7 cut(s) 240, 286, 360, 422, 458, 573, 596
HphI GGTGA 2 cut(s) 335, 409
Hpy166II GTNNAC 6 cut(s) 61, 136, 202, 382, 475, 635
Hpy188I TCNGA 2 cut(s) 314, 787
Hpy188III TCNNGA 2 cut(s) 52, 468
Hpy8I GTNNAC 6 cut(s) 61, 136, 202, 382, 475, 635
Hpy99I CGWCG 6 cut(s) 98, 157, 209, 299, 314, 782
HpyAV CCTTC 2 cut(s) 579, 784
HpyCH4III ACNGT 2 cut(s) 397, 608
HpyCH4IV ACGT 7 cut(s) 57, 152, 297, 309, 384, 554, 780
HpyCH4V TGCA 5 cut(s) 235, 563, 640, 728, 773
HpyF10VI GCNNNNNNNGC 6 cut(s) 333, 619, 646, 704, 725, 746
HpyF3I CTNAG 4 cut(s) 246, 466, 618, 741
HpySE526I ACGT 7 cut(s) 57, 152, 297, 309, 384, 554, 780
Hsp92I GRCGYC 3 cut(s) 5, 309, 384
Hsp92II CATG 3 cut(s) 217, 239, 482
HspAI GCGC 5 cut(s) 327, 409, 419, 437, 719
Kzo9I GATC 1 cut(s) 272
LguI GCTCTTC 1 cut(s) 659
LmnI GCTCC 6 cut(s) 242, 288, 294, 403, 424, 487
Lsp1109I GCAGC 1 cut(s) 547
MaeII ACGT 7 cut(s) 57, 152, 297, 309, 384, 554, 780
MaeIII GTNAC 1 cut(s) 391
MalI GATC 1 cut(s) 274
MboI GATC 1 cut(s) 272
MboII GAAGA 5 cut(s) 332, 332, 670, 673, 676
MflI RGATCY 1 cut(s) 272
MhlI GDGCHC 1 cut(s) 252
MluCI AATT 2 cut(s) 768, 802
MlyI GAGTC 1 cut(s) 586
MmeI TCCRAC 3 cut(s) 46, 292, 765
MslI CAYNNNNRTG 1 cut(s) 131
MspA1I CMGCKG 1 cut(s) 265
MspI CCGG 7 cut(s) 240, 286, 360, 422, 458, 573, 596
MspR9I CCNGG 1 cut(s) 574
MvnI CGCG 4 cut(s) 294, 327, 409, 590
MwoI GCNNNNNNNGC 6 cut(s) 333, 619, 646, 704, 725, 746
NciI CCSGG 1 cut(s) 574
NdeII GATC 1 cut(s) 272
NlaIII CATG 3 cut(s) 217, 239, 482
NlaIV GGNNCC 5 cut(s) 76, 284, 290, 405, 453
NmeAIII GCCGAG 2 cut(s) 135, 259
NspI RCATGY 1 cut(s) 239
OliI CACNNNNGTG 1 cut(s) 131
PaeI GCATGC 1 cut(s) 239
PciSI GCTCTTC 1 cut(s) 659
PcsI WCGNNNNNNNCGW 2 cut(s) 102, 144
Pfl23II CGTACG 2 cut(s) 87, 555
PflFI GACNNNGTC 1 cut(s) 299
PflMI CCANNNNNTGG 3 cut(s) 503, 545, 629
PkrI GCNGC 5 cut(s) 335, 408, 441, 495, 562
PleI GAGTC 1 cut(s) 586
PpsI GAGTC 1 cut(s) 586
PpuMI RGGWCCY 1 cut(s) 451
Psp5II RGGWCCY 1 cut(s) 451
PspFI CCCAGC 1 cut(s) 721
PspLI CGTACG 2 cut(s) 87, 555
PspN4I GGNNCC 5 cut(s) 76, 284, 290, 405, 453
PspPI GGNCC 3 cut(s) 74, 354, 451
PspPPI RGGWCCY 1 cut(s) 451
PstI CTGCAG 1 cut(s) 642
PsuI RGATCY 1 cut(s) 272
PsyI GACNNNGTC 1 cut(s) 299
PvuII CAGCTG 1 cut(s) 265
RsaI GTAC 4 cut(s) 89, 557, 605, 644
RsaNI GTAC 4 cut(s) 88, 556, 604, 643
RseI CAYNNNNRTG 1 cut(s) 131
SapI GCTCTTC 1 cut(s) 659
SatI GCNGC 5 cut(s) 334, 407, 440, 494, 561
Sau3AI GATC 1 cut(s) 272
Sau96I GGNCC 3 cut(s) 74, 354, 451
SchI GAGTC 1 cut(s) 586
ScrFI CCNGG 1 cut(s) 574
SduI GDGCHC 1 cut(s) 252
SfcI CTRYAG 1 cut(s) 638
SinI GGWCC 1 cut(s) 451
SmiMI CAYNNNNRTG 1 cut(s) 131
SmlI CTYRAG 1 cut(s) 50
SmoI CTYRAG 1 cut(s) 50
SphI GCATGC 1 cut(s) 239
Sse9I AATT 2 cut(s) 768, 802
StyD4I CCNGG 1 cut(s) 572
StyI CCWWGG 1 cut(s) 129
TaaI ACNGT 2 cut(s) 397, 608
TaiI ACGT 7 cut(s) 60, 155, 300, 312, 387, 557, 783
TaqI TCGA 3 cut(s) 105, 225, 303
TasI AATT 2 cut(s) 768, 802
TauI GCSGC 4 cut(s) 336, 409, 442, 496
TscAI CASTG 1 cut(s) 546
TseI GCWGC 1 cut(s) 560
TspDTI ATGAA 1 cut(s) 333
TspRI CASTG 1 cut(s) 546
Tth111I GACNNNGTC 1 cut(s) 299
Van91I CCANNNNNTGG 3 cut(s) 503, 545, 629
VpaK11BI GGWCC 1 cut(s) 451
XagI CCTNNNNNAGG 1 cut(s) 361
XceI RCATGY 1 cut(s) 239
XcmI CCANNNNNNNNNTGG 1 cut(s) 265
ZraI GACGTC 2 cut(s) 310, 385
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.