RLG00000015211

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0004046
Physical Location & Seq
Reverse (-)
63132811 .. 63133635
825 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015211

Sequence Viewer

Length: 825 bp
ATGGCTTCCGGCTACGAAGTAGAGGTCACAATTTCTTCCGCCAAAGACCTCAAAAACGTGAATTGGCGCCACGGAGAGCTGAAGCCCTACGCCGTTGTTTGGGTGGACCCAAGTAACAAATGCTCCACCCATGTGGACGAATACGGCGACGAGTCGCCTACGTGGGACGAGACACTCTCGATTCCGTTACGGGGTCCTGTCGAGGACTCGACCCTTTTTGTGGACATCATCCACGTCAAAGCGGACGAGGGCGTCAAGCCATTGATCGGGTCCGCAAAACTCCCGCTTAGGGAGGTCCTCGATGATGTGGGGCTCGGAGCGCAGTCCGTTCACAAGTTAAAGCTCAAGCGGCCCTCGGGTAGGCCCCACGGAAAGTTGGAAGTCAAAGTCACAGTCCGCCCGCCCCGTTACCAAGCACCCGACCCATATCAGGTCCGCGCGCCCTACGGGTACGGGGTCCAACCGCCGCAGTCGAGAGACTACGGCGCACCTCAGCAGTACAATTACCAATATGCCCCTCCCGCCCAACCCCAATATTACCAAAGCAGCGGCGTCCCACAAGCAGGGTACGGTTACAATCAGCCATCTTACGATCAGAGTCAGTATGGGAGTGGCGGGTCGTACGGGTACGGGTCGTCGGAGAAGCCGAAGGAGAAGAGCAAGTTTGGGGTCGGGACCGGATTGGCTGTGGGAGCCGTAGCCGGAGTGTTGGGTGGACTTGCGATTGCTGAAGGAGTGGATTACGTGGAGGACAAGATTGCTGATGATGCAGCGGAGAAAGTGGAGGAGGATCTTGGCTATGATGACTTTGGCGATGATGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

275

Amino Acids

29.76

Weight (kDa)

4.85

Isoelectric Point (pI)

34.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 7 - 100 1.2e-10 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 251
AccB1I GGYRCC 1 cut(s) 66
AccII CGCG 2 cut(s) 438, 440
AclWI GGATC 1 cut(s) 798
AcuI CTGAAG 2 cut(s) 101, 750
AcyI GRCGYC 3 cut(s) 67, 252, 552
AfaI GTAC 5 cut(s) 452, 500, 569, 623, 629
AfiI CCNNNNNNNGG 8 cut(s) 99, 191, 220, 266, 289, 360, 430, 563
AjiI CACGTC 1 cut(s) 235
AleI CACNNNNGTG 1 cut(s) 131
AluBI AGCT 2 cut(s) 79, 343
AluI AGCT 2 cut(s) 79, 343
Alw26I GTCTC 2 cut(s) 164, 471
AlwI GGATC 1 cut(s) 798
Ama87I CYCGRG 1 cut(s) 355
AoxI GGCC 2 cut(s) 350, 362
ApeKI GCWGC 2 cut(s) 546, 770
ArsI GACNNNNNNTTYG 4 cut(s) 9, 41, 378, 410
AspLEI GCGC 5 cut(s) 69, 322, 440, 442, 488
AspS9I GGNCC 9 cut(s) 106, 194, 270, 295, 351, 363, 433, 457, 675
AvaI CYCGRG 1 cut(s) 355
AvaII GGWCC 7 cut(s) 106, 194, 270, 295, 433, 457, 675
BanI GGYRCC 1 cut(s) 66
BanII GRGCYC 1 cut(s) 315
BbvCI CCTCAGC 1 cut(s) 492
BbvI GCAGC 2 cut(s) 558, 782
BccI CCATC 1 cut(s) 592
BceAI ACGGC 4 cut(s) 77, 160, 499, 680
BcoDI GTCTC 2 cut(s) 164, 471
BfoI RGCGCY 1 cut(s) 70
BisI GCNGC 5 cut(s) 350, 467, 547, 550, 771
BlsI GCNGC 5 cut(s) 351, 468, 548, 551, 772
Bme18I GGWCC 7 cut(s) 106, 194, 270, 295, 433, 457, 675
BmeT110I CYCGRG 1 cut(s) 355
BmgBI CACGTC 1 cut(s) 235
BmgT120I GGNCC 9 cut(s) 106, 194, 270, 295, 351, 363, 433, 457, 675
BmiI GGNNCC 8 cut(s) 68, 108, 195, 271, 365, 458, 676, 694
BmsI GCATC 1 cut(s) 757
BplI GAGNNNNNCTC 2 cut(s) 161, 193
Bpu10I CCTNAGC 2 cut(s) 287, 492
BpuEI CTTGAG 1 cut(s) 329
BsaAI YACGTR 2 cut(s) 162, 745
BsaHI GRCGYC 3 cut(s) 67, 252, 552
BsaJI CCNNGG 3 cut(s) 70, 354, 367
BsaWI WCCGGW 1 cut(s) 677
BsaXI ACNNNNNCTCC 2 cut(s) 107, 137
Bsc4I CCNNNNNNNGG 8 cut(s) 99, 191, 220, 266, 289, 360, 430, 563
BseDI CCNNGG 3 cut(s) 70, 354, 367
BseGI GGATG 1 cut(s) 228
BseLI CCNNNNNNNGG 8 cut(s) 99, 191, 220, 266, 289, 360, 430, 563
BseMII CTCAG 1 cut(s) 506
BsePI GCGCGC 1 cut(s) 438
BseRI GAGGAG 1 cut(s) 800
BseXI GCAGC 2 cut(s) 558, 782
Bsh1236I CGCG 2 cut(s) 438, 440
BshFI GGCC 2 cut(s) 352, 364
BshNI GGYRCC 1 cut(s) 66
BsiHKCI CYCGRG 1 cut(s) 355
BsiSI CCGG 3 cut(s) 9, 678, 702
BsiWI CGTACG 1 cut(s) 621
BslFI GGGAC 3 cut(s) 179, 539, 688
BslI CCNNNNNNNGG 8 cut(s) 99, 191, 220, 266, 289, 360, 430, 563
BsmAI GTCTC 2 cut(s) 164, 471
BsmFI GGGAC 3 cut(s) 179, 539, 688
BsnI GGCC 2 cut(s) 352, 364
BsoBI CYCGRG 1 cut(s) 355
Bsp1286I GDGCHC 1 cut(s) 315
Bsp143I GATC 3 cut(s) 264, 592, 790
BspANI GGCC 2 cut(s) 352, 364
BspCNI CTCAG 1 cut(s) 505
BspFNI CGCG 2 cut(s) 438, 440
BspLI GGNNCC 8 cut(s) 68, 108, 195, 271, 365, 458, 676, 694
BspPI GGATC 1 cut(s) 798
BspQI GCTCTTC 1 cut(s) 650
BspT107I GGYRCC 1 cut(s) 66
BssECI CCNNGG 3 cut(s) 70, 354, 367
BssHII GCGCGC 1 cut(s) 438
BssMI GATC 3 cut(s) 264, 592, 790
BssNI GRCGYC 3 cut(s) 67, 252, 552
Bst4CI ACNGT 2 cut(s) 394, 572
Bst6I CTCTTC 1 cut(s) 650
BstACI GRCGYC 3 cut(s) 67, 252, 552
BstBAI YACGTR 2 cut(s) 162, 745
BstC8I GCNNGC 2 cut(s) 401, 440
BstDEI CTNAG 2 cut(s) 287, 492
BstDSI CCRYGG 2 cut(s) 70, 367
BstENI CCTNNNNNAGG 1 cut(s) 358
BstF5I GGATG 1 cut(s) 228
BstFNI CGCG 2 cut(s) 438, 440
BstH2I RGCGCY 1 cut(s) 70
BstHHI GCGC 5 cut(s) 69, 322, 440, 442, 488
BstKTI GATC 3 cut(s) 267, 595, 793
BstMAI GTCTC 2 cut(s) 164, 471
BstMBI GATC 3 cut(s) 264, 592, 790
BstMWI GCNNNNNNNGC 5 cut(s) 319, 349, 521, 692, 767
BstUI CGCG 2 cut(s) 438, 440
BstV1I GCAGC 2 cut(s) 558, 782
BstX2I RGATCY 1 cut(s) 790
BstXI CCANNNNNNTGG 1 cut(s) 133
BstYI RGATCY 1 cut(s) 790
BsuRI GGCC 2 cut(s) 352, 364
BtgI CCRYGG 2 cut(s) 70, 367
BtrI CACGTC 1 cut(s) 235
BtsCI GGATG 1 cut(s) 228
Cac8I GCNNGC 2 cut(s) 401, 440
CfoI GCGC 5 cut(s) 69, 322, 440, 442, 488
Cfr13I GGNCC 9 cut(s) 106, 194, 270, 295, 351, 363, 433, 457, 675
CseI GACGC 2 cut(s) 241, 541
Csp6I GTAC 5 cut(s) 451, 499, 568, 622, 628
CviAII CATG 1 cut(s) 131
CviQI GTAC 5 cut(s) 451, 499, 568, 622, 628
DdeI CTNAG 2 cut(s) 287, 492
DinI GGCGCC 1 cut(s) 68
DpnI GATC 3 cut(s) 266, 594, 792
DpnII GATC 3 cut(s) 264, 592, 790
DrdI GACNNNNNNGTC 1 cut(s) 251
DseDI GACNNNNNNGTC 1 cut(s) 251
Eam1104I CTCTTC 1 cut(s) 650
EarI CTCTTC 1 cut(s) 650
EciI GGCGGA 2 cut(s) 28, 386
Eco24I GRGCYC 1 cut(s) 315
Eco47I GGWCC 7 cut(s) 106, 194, 270, 295, 433, 457, 675
Eco57I CTGAAG 2 cut(s) 101, 750
Eco88I CYCGRG 1 cut(s) 355
EcoNI CCTNNNNNAGG 1 cut(s) 358
EcoO109I RGGNCCY 3 cut(s) 194, 295, 363
EcoT38I GRGCYC 1 cut(s) 315
EgeI GGCGCC 1 cut(s) 68
EheI GGCGCC 1 cut(s) 68
FaeI CATG 1 cut(s) 134
FaiI YATR 5 cut(s) 132, 427, 513, 606, 801
FaqI GGGAC 3 cut(s) 179, 539, 688
FatI CATG 1 cut(s) 130
FauI CCCGC 4 cut(s) 291, 408, 529, 608
Fnu4HI GCNGC 5 cut(s) 350, 467, 547, 550, 771
FokI GGATG 1 cut(s) 215
FriOI GRGCYC 1 cut(s) 315
Fsp4HI GCNGC 5 cut(s) 350, 467, 547, 550, 771
GlaI GCGC 5 cut(s) 68, 321, 439, 441, 487
GluI GCNGC 5 cut(s) 350, 467, 547, 550, 771
HaeII RGCGCY 1 cut(s) 70
HaeIII GGCC 2 cut(s) 352, 364
HapII CCGG 3 cut(s) 9, 678, 702
HgaI GACGC 2 cut(s) 241, 541
HhaI GCGC 5 cut(s) 69, 322, 440, 442, 488
Hin1I GRCGYC 3 cut(s) 67, 252, 552
Hin1II CATG 1 cut(s) 134
Hin6I GCGC 5 cut(s) 67, 320, 438, 440, 486
HinP1I GCGC 5 cut(s) 67, 320, 438, 440, 486
HinfI GANTC 4 cut(s) 152, 181, 206, 598
HpaII CCGG 3 cut(s) 9, 678, 702
Hpy166II GTNNAC 5 cut(s) 106, 136, 223, 331, 716
Hpy188I TCNGA 3 cut(s) 317, 597, 640
Hpy188III TCNNGA 3 cut(s) 178, 474, 673
Hpy8I GTNNAC 5 cut(s) 106, 136, 223, 331, 716
Hpy99I CGWCG 2 cut(s) 152, 640
HpyAV CCTTC 2 cut(s) 643, 725
HpyCH4III ACNGT 2 cut(s) 394, 572
HpyCH4IV ACGT 4 cut(s) 57, 161, 234, 744
HpyCH4V TGCA 1 cut(s) 770
HpyF10VI GCNNNNNNNGC 5 cut(s) 319, 349, 521, 692, 767
HpyF3I CTNAG 2 cut(s) 287, 492
HpySE526I ACGT 4 cut(s) 57, 161, 234, 744
Hsp92I GRCGYC 3 cut(s) 67, 252, 552
Hsp92II CATG 1 cut(s) 134
HspAI GCGC 5 cut(s) 67, 320, 438, 440, 486
KasI GGCGCC 1 cut(s) 66
Kzo9I GATC 3 cut(s) 264, 592, 790
LguI GCTCTTC 1 cut(s) 650
LmnI GCTCC 3 cut(s) 128, 317, 692
LpnPI CCDG 6 cut(s) 22, 210, 416, 549, 691, 715
Lsp1109I GCAGC 2 cut(s) 558, 782
LweI GCATC 1 cut(s) 757
MaeII ACGT 4 cut(s) 57, 161, 234, 744
MaeIII GTNAC 6 cut(s) 25, 113, 186, 388, 407, 572
MalI GATC 3 cut(s) 266, 594, 792
MboI GATC 3 cut(s) 264, 592, 790
MboII GAAGA 2 cut(s) 27, 667
MflI RGATCY 1 cut(s) 790
MhlI GDGCHC 1 cut(s) 315
MluCI AATT 3 cut(s) 30, 61, 502
Mly113I GGCGCC 1 cut(s) 67
MlyI GAGTC 3 cut(s) 161, 200, 607
MmeI TCCRAC 3 cut(s) 357, 484, 618
MseI TTAA 1 cut(s) 338
MslI CAYNNNNRTG 1 cut(s) 131
MspA1I CMGCKG 2 cut(s) 549, 773
MspI CCGG 3 cut(s) 9, 678, 702
MvnI CGCG 2 cut(s) 438, 440
MwoI GCNNNNNNNGC 5 cut(s) 319, 349, 521, 692, 767
NarI GGCGCC 1 cut(s) 67
NdeII GATC 3 cut(s) 264, 592, 790
NlaIII CATG 1 cut(s) 134
NlaIV GGNNCC 8 cut(s) 68, 108, 195, 271, 365, 458, 676, 694
NmuCI GTSAC 2 cut(s) 25, 388
OliI CACNNNNGTG 1 cut(s) 131
PauI GCGCGC 1 cut(s) 438
PciSI GCTCTTC 1 cut(s) 650
PcsI WCGNNNNNNNCGW 2 cut(s) 144, 644
PfeI GAWTC 1 cut(s) 181
Pfl23II CGTACG 1 cut(s) 621
PkrI GCNGC 5 cut(s) 351, 468, 548, 551, 772
PleI GAGTC 3 cut(s) 160, 200, 606
PluTI GGCGCC 1 cut(s) 70
PpsI GAGTC 3 cut(s) 160, 200, 606
Ppu21I YACGTR 2 cut(s) 162, 745
PpuMI RGGWCCY 2 cut(s) 194, 295
Psp5II RGGWCCY 2 cut(s) 194, 295
PspLI CGTACG 1 cut(s) 621
PspN4I GGNNCC 8 cut(s) 68, 108, 195, 271, 365, 458, 676, 694
PspPI GGNCC 9 cut(s) 106, 194, 270, 295, 351, 363, 433, 457, 675
PspPPI RGGWCCY 2 cut(s) 194, 295
PsuI RGATCY 1 cut(s) 790
PteI GCGCGC 1 cut(s) 438
RsaI GTAC 5 cut(s) 452, 500, 569, 623, 629
RsaNI GTAC 5 cut(s) 451, 499, 568, 622, 628
RseI CAYNNNNRTG 1 cut(s) 131
SapI GCTCTTC 1 cut(s) 650
SaqAI TTAA 1 cut(s) 338
SatI GCNGC 5 cut(s) 350, 467, 547, 550, 771
Sau3AI GATC 3 cut(s) 264, 592, 790
Sau96I GGNCC 9 cut(s) 106, 194, 270, 295, 351, 363, 433, 457, 675
SchI GAGTC 3 cut(s) 161, 200, 607
SduI GDGCHC 1 cut(s) 315
SfaNI GCATC 1 cut(s) 757
SfoI GGCGCC 1 cut(s) 68
SinI GGWCC 7 cut(s) 106, 194, 270, 295, 433, 457, 675
SmiMI CAYNNNNRTG 1 cut(s) 131
SmlI CTYRAG 1 cut(s) 344
SmoI CTYRAG 1 cut(s) 344
Sse9I AATT 3 cut(s) 30, 61, 502
SspDI GGCGCC 1 cut(s) 66
SspI AATATT 1 cut(s) 536
TaaI ACNGT 2 cut(s) 394, 572
TaiI ACGT 4 cut(s) 60, 164, 237, 747
TaqI TCGA 5 cut(s) 179, 201, 209, 300, 473
TasI AATT 3 cut(s) 30, 61, 502
TatI WGTACW 1 cut(s) 498
TauI GCSGC 3 cut(s) 352, 469, 552
TfiI GAWTC 1 cut(s) 181
Tru1I TTAA 1 cut(s) 338
Tru9I TTAA 1 cut(s) 338
TseFI GTSAC 2 cut(s) 25, 388
TseI GCWGC 2 cut(s) 546, 770
Tsp45I GTSAC 2 cut(s) 25, 388
TspGWI ACGGA 4 cut(s) 87, 174, 316, 384
VpaK11BI GGWCC 7 cut(s) 106, 194, 270, 295, 433, 457, 675
XagI CCTNNNNNAGG 1 cut(s) 358
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.