MD07G1276700.v1.1

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0004046
Physical Location & Seq
Reverse (-)
34224895 .. 34225773
879 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1276700.v1.1.491

Sequence Viewer

Length: 879 bp
ATGGCGTCCGGTCACGAAGTTCAAGTGAAGCTGACCTCCGCCCACGACCTTAAGAACGTGAACTGGCGAAACGGCGCCGTCAGGCCCTACGCCGTCGTTTGGGTGGACCCCAAGAGAAAATGCTCCAGCCGCGTCGACGAGGAAGGCGACACATCACCCTACTGGGACGAGACCCTCACCATCCCCTTACCCGGCCCCGTCGACGGCGACACCACTCTCCACGTCGACATAGTCCACGCCGGATCCGACCCGGACACCAAGCCCCTGATCGGGTCGGCCCGGCTCAAACTCCGCGAGGTCCTCGATGACGTGGGCTTCGACGAGCGCGCCAGCCGCACTCTCCATCTCAAGCGACCATCCGGTAGGCCCCACGGGAAGGTTGACGTTAAGGTTACTATTAGGAAGCCACGCCCTCGTGTAGCGGATCCCTACTACGCTCCGCCTTACGGTGTCCCACCGCCAGCTGGCTCTCGGGATTTGCCGACTCCTCCTCCGGCTTACGGGGCACCTTACGGCGCACCTGCTCCTCCGGCGCCATACAATCCTTACTACCCGGCGGCTCCGCCGTCTGGTTACCCGTATAACGGGTACAATGCTGCTCCGCCGCAGTACGGTCAGCCGGCTCCTTACGGTCAGACGGGACAGGGGAGTTACGGGCAGCCGGGGCAGGGTTATGGGCAGGGATCGTACGGACAGGAAGAGAAGAAGAAGAGCAAGTTTGGGGGGATGGGGACGGGGACAGGACTGGCGGTGGGAGCGGTGGCGGGAGTGCTGGGTGGGCTGGCATTGGCGGAGGGTTTTGAGTACATGGAGGACAAAATTGCAGATGACGCTGCTGAGAAAGTGGAGGATGATCTGTACGACGGCGATGACTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

293

Amino Acids

31.18

Weight (kDa)

5.41

Isoelectric Point (pI)

32.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 6 - 102 1.2e-11 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 529
Acc36I ACCTGC 1 cut(s) 529
AccB1I GGYRCC 3 cut(s) 74, 505, 532
AccBSI CCGCTC 1 cut(s) 758
AccI GTMKAC 3 cut(s) 135, 201, 225
AccII CGCG 3 cut(s) 132, 294, 327
AclWI GGATC 5 cut(s) 237, 250, 419, 432, 691
AcyI GRCGYC 3 cut(s) 5, 75, 533
AfaI GTAC 5 cut(s) 590, 611, 689, 806, 860
AflII CTTAAG 1 cut(s) 50
AgsI TTSAA 1 cut(s) 23
AjiI CACGTC 2 cut(s) 223, 310
AluBI AGCT 2 cut(s) 31, 464
AluI AGCT 2 cut(s) 31, 464
Alw26I GTCTC 1 cut(s) 164
AlwI GGATC 5 cut(s) 237, 250, 419, 432, 691
Ama87I CYCGRG 1 cut(s) 471
AoxI GGCC 4 cut(s) 83, 193, 276, 365
ApeKI GCWGC 3 cut(s) 596, 658, 833
ArsI GACNNNNNNTTYG 2 cut(s) 299, 331
AspLEI GCGC 5 cut(s) 77, 327, 329, 518, 535
AspS9I GGNCC 6 cut(s) 84, 106, 194, 277, 298, 366
AsuC2I CCSGG 5 cut(s) 192, 251, 280, 554, 663
AsuHPI GGTGA 2 cut(s) 147, 169
AvaI CYCGRG 1 cut(s) 471
AvaII GGWCC 2 cut(s) 106, 298
BaeGI GKGCMC 1 cut(s) 508
BamHI GGATCC 2 cut(s) 242, 424
BanI GGYRCC 3 cut(s) 74, 505, 532
BauI CACGAG 1 cut(s) 414
BbvI GCAGC 3 cut(s) 583, 670, 820
BccI CCATC 4 cut(s) 188, 351, 364, 721
BceAI ACGGC 6 cut(s) 62, 77, 88, 220, 529, 550
BcnI CCSGG 5 cut(s) 192, 251, 280, 554, 663
BcoDI GTCTC 1 cut(s) 164
BfaI CTAG 1 cut(s) 877
BfoI RGCGCY 2 cut(s) 78, 536
BfrI CTTAAG 1 cut(s) 50
BfuAI ACCTGC 1 cut(s) 529
BisI GCNGC 7 cut(s) 130, 334, 558, 597, 605, 659, 834
BlsI GCNGC 7 cut(s) 131, 335, 559, 598, 606, 660, 835
Bme1390I CCNGG 5 cut(s) 192, 251, 280, 554, 663
Bme18I GGWCC 2 cut(s) 106, 298
BmeT110I CYCGRG 1 cut(s) 471
BmgBI CACGTC 2 cut(s) 223, 310
BmgT120I GGNCC 6 cut(s) 84, 106, 194, 277, 298, 366
BmrFI CCNGG 5 cut(s) 192, 251, 280, 554, 663
BmrI ACTGGG 1 cut(s) 172
BmuI ACTGGG 1 cut(s) 172
BpmI CTGGAG 1 cut(s) 109
BpuEI CTTGAG 1 cut(s) 332
BpuMI CCSGG 5 cut(s) 192, 251, 280, 554, 663
BsaHI GRCGYC 3 cut(s) 5, 75, 533
BsaI GGTCTC 1 cut(s) 164
BsaJI CCNNGG 2 cut(s) 370, 662
BsaWI WCCGGW 2 cut(s) 8, 359
BsaXI ACNNNNNCTCC 2 cut(s) 475, 505
Bse118I RCCGGY 1 cut(s) 619
Bse1I ACTGG 3 cut(s) 68, 167, 750
BseDI CCNNGG 2 cut(s) 370, 662
BseGI GGATG 4 cut(s) 180, 356, 732, 856
BseMII CTCAG 1 cut(s) 828
BseNI ACTGG 3 cut(s) 68, 167, 750
BsePI GCGCGC 1 cut(s) 325
BseRI GAGGAG 3 cut(s) 477, 480, 516
BseSI GKGCMC 1 cut(s) 508
BseXI GCAGC 3 cut(s) 583, 670, 820
BseYI CCCAGC 1 cut(s) 772
Bsh1236I CGCG 3 cut(s) 132, 294, 327
BshFI GGCC 4 cut(s) 85, 195, 278, 367
BshNI GGYRCC 3 cut(s) 74, 505, 532
BsiHKCI CYCGRG 1 cut(s) 471
BsiWI CGTACG 1 cut(s) 687
BslFI GGGAC 5 cut(s) 179, 437, 654, 745, 751
BsmAI GTCTC 1 cut(s) 164
BsmFI GGGAC 5 cut(s) 179, 437, 654, 745, 751
BsnI GGCC 4 cut(s) 85, 195, 278, 367
Bso31I GGTCTC 1 cut(s) 164
BsoBI CYCGRG 1 cut(s) 471
Bsp1286I GDGCHC 1 cut(s) 508
Bsp143I GATC 5 cut(s) 242, 267, 424, 683, 853
BspANI GGCC 4 cut(s) 85, 195, 278, 367
BspCNI CTCAG 1 cut(s) 829
BspFNI CGCG 3 cut(s) 132, 294, 327
BspMI ACCTGC 1 cut(s) 529
BspPI GGATC 5 cut(s) 237, 250, 419, 432, 691
BspQI GCTCTTC 1 cut(s) 704
BspT107I GGYRCC 3 cut(s) 74, 505, 532
BspTI CTTAAG 1 cut(s) 50
BspTNI GGTCTC 1 cut(s) 164
BsrBI CCGCTC 1 cut(s) 758
BsrFI RCCGGY 1 cut(s) 619
BsrI ACTGG 3 cut(s) 68, 167, 750
BssAI RCCGGY 1 cut(s) 619
BssECI CCNNGG 2 cut(s) 370, 662
BssHII GCGCGC 1 cut(s) 325
BssMI GATC 5 cut(s) 242, 267, 424, 683, 853
BssNI GRCGYC 3 cut(s) 5, 75, 533
BssSI CACGAG 1 cut(s) 414
Bst2BI CACGAG 1 cut(s) 414
Bst4CI ACNGT 3 cut(s) 449, 614, 632
Bst6I CTCTTC 2 cut(s) 693, 704
BstACI GRCGYC 3 cut(s) 5, 75, 533
BstAFI CTTAAG 1 cut(s) 50
BstC8I GCNNGC 6 cut(s) 327, 331, 462, 466, 621, 783
BstDEI CTNAG 1 cut(s) 837
BstDSI CCRYGG 1 cut(s) 370
BstEII GGTNACC 1 cut(s) 572
BstF5I GGATG 4 cut(s) 180, 356, 732, 856
BstFNI CGCG 3 cut(s) 132, 294, 327
BstH2I RGCGCY 2 cut(s) 78, 536
BstHHI GCGC 5 cut(s) 77, 327, 329, 518, 535
BstKTI GATC 5 cut(s) 245, 270, 427, 686, 856
BstMAI GTCTC 1 cut(s) 164
BstMBI GATC 5 cut(s) 242, 267, 424, 683, 853
BstMWI GCNNNNNNNGC 8 cut(s) 129, 333, 503, 530, 664, 755, 778, 830
BstPI GGTNACC 1 cut(s) 572
BstSCI CCNGG 5 cut(s) 190, 249, 278, 552, 661
BstSLI GKGCMC 1 cut(s) 508
BstUI CGCG 3 cut(s) 132, 294, 327
BstV1I GCAGC 3 cut(s) 583, 670, 820
BstX2I RGATCY 2 cut(s) 242, 424
BstYI RGATCY 2 cut(s) 242, 424
BsuRI GGCC 4 cut(s) 85, 195, 278, 367
BtgI CCRYGG 1 cut(s) 370
BtrI CACGTC 2 cut(s) 223, 310
BtsCI GGATG 4 cut(s) 180, 356, 732, 856
BveI ACCTGC 1 cut(s) 529
Cac8I GCNNGC 6 cut(s) 327, 331, 462, 466, 621, 783
CfoI GCGC 5 cut(s) 77, 327, 329, 518, 535
Cfr10I RCCGGY 1 cut(s) 619
Cfr13I GGNCC 6 cut(s) 84, 106, 194, 277, 298, 366
CseI GACGC 2 cut(s) 121, 839
Csp6I GTAC 5 cut(s) 589, 610, 688, 805, 859
CviAII CATG 1 cut(s) 808
CviQI GTAC 5 cut(s) 589, 610, 688, 805, 859
DdeI CTNAG 1 cut(s) 837
DinI GGCGCC 2 cut(s) 76, 534
DpnI GATC 5 cut(s) 244, 269, 426, 685, 855
DpnII GATC 5 cut(s) 242, 267, 424, 683, 853
Eam1104I CTCTTC 2 cut(s) 693, 704
EarI CTCTTC 2 cut(s) 693, 704
EciI GGCGGA 5 cut(s) 28, 429, 552, 591, 806
Eco31I GGTCTC 1 cut(s) 164
Eco47I GGWCC 2 cut(s) 106, 298
Eco88I CYCGRG 1 cut(s) 471
Eco91I GGTNACC 1 cut(s) 572
EcoO109I RGGNCCY 3 cut(s) 84, 298, 366
EcoO65I GGTNACC 1 cut(s) 572
EgeI GGCGCC 2 cut(s) 76, 534
EheI GGCGCC 2 cut(s) 76, 534
FaeI CATG 1 cut(s) 811
FaiI YATR 5 cut(s) 230, 538, 582, 675, 809
FaqI GGGAC 5 cut(s) 179, 437, 654, 745, 751
FatI CATG 1 cut(s) 807
FauI CCCGC 1 cut(s) 757
FblI GTMKAC 3 cut(s) 135, 201, 225
Fnu4HI GCNGC 7 cut(s) 130, 334, 558, 597, 605, 659, 834
FokI GGATG 4 cut(s) 167, 343, 739, 863
Fsp4HI GCNGC 7 cut(s) 130, 334, 558, 597, 605, 659, 834
FspBI CTAG 1 cut(s) 877
GlaI GCGC 5 cut(s) 76, 326, 328, 517, 534
GluI GCNGC 7 cut(s) 130, 334, 558, 597, 605, 659, 834
GsaI CCCAGC 1 cut(s) 776
GsuI CTGGAG 1 cut(s) 109
HaeII RGCGCY 2 cut(s) 78, 536
HaeIII GGCC 4 cut(s) 85, 195, 278, 367
HgaI GACGC 2 cut(s) 121, 839
HhaI GCGC 5 cut(s) 77, 327, 329, 518, 535
Hin1I GRCGYC 3 cut(s) 5, 75, 533
Hin1II CATG 1 cut(s) 811
Hin6I GCGC 5 cut(s) 75, 325, 327, 516, 533
HinP1I GCGC 5 cut(s) 75, 325, 327, 516, 533
HincII GTYRAC 4 cut(s) 136, 202, 226, 382
HindII GTYRAC 4 cut(s) 136, 202, 226, 382
HinfI GANTC 1 cut(s) 484
HphI GGTGA 2 cut(s) 147, 169
Hpy166II GTNNAC 7 cut(s) 61, 106, 136, 202, 226, 235, 382
Hpy188I TCNGA 2 cut(s) 247, 636
Hpy188III TCNNGA 2 cut(s) 14, 473
Hpy8I GTNNAC 7 cut(s) 61, 106, 136, 202, 226, 235, 382
Hpy99I CGWCG 8 cut(s) 98, 137, 140, 203, 206, 227, 323, 866
HpyAV CCTTC 2 cut(s) 137, 370
HpyCH4III ACNGT 3 cut(s) 449, 614, 632
HpyCH4IV ACGT 4 cut(s) 57, 222, 309, 384
HpyCH4V TGCA 1 cut(s) 824
HpyF10VI GCNNNNNNNGC 8 cut(s) 129, 333, 503, 530, 664, 755, 778, 830
HpyF3I CTNAG 1 cut(s) 837
HpySE526I ACGT 4 cut(s) 57, 222, 309, 384
Hsp92I GRCGYC 3 cut(s) 5, 75, 533
Hsp92II CATG 1 cut(s) 811
HspAI GCGC 5 cut(s) 75, 325, 327, 516, 533
KasI GGCGCC 2 cut(s) 74, 532
KroI GCCGGC 1 cut(s) 619
KroNI GCCGGC 1 cut(s) 621
Kzo9I GATC 5 cut(s) 242, 267, 424, 683, 853
LguI GCTCTTC 1 cut(s) 704
LmnI GCTCC 7 cut(s) 128, 442, 529, 565, 604, 628, 755
Lsp1109I GCAGC 3 cut(s) 583, 670, 820
MaeI CTAG 1 cut(s) 877
MaeII ACGT 4 cut(s) 57, 222, 309, 384
MaeIII GTNAC 4 cut(s) 11, 391, 572, 650
MalI GATC 5 cut(s) 244, 269, 426, 685, 855
MbiI CCGCTC 1 cut(s) 758
MboI GATC 5 cut(s) 242, 267, 424, 683, 853
MboII GAAGA 4 cut(s) 710, 715, 718, 721
MflI RGATCY 2 cut(s) 242, 424
MhlI GDGCHC 1 cut(s) 508
MluCI AATT 1 cut(s) 819
Mly113I GGCGCC 2 cut(s) 75, 533
MlyI GAGTC 1 cut(s) 478
MmeI TCCRAC 1 cut(s) 270
MroNI GCCGGC 1 cut(s) 619
MseI TTAA 2 cut(s) 51, 387
MspA1I CMGCKG 1 cut(s) 464
MspCI CTTAAG 1 cut(s) 50
MspR9I CCNGG 5 cut(s) 192, 251, 280, 554, 663
MvnI CGCG 3 cut(s) 132, 294, 327
MwoI GCNNNNNNNGC 8 cut(s) 129, 333, 503, 530, 664, 755, 778, 830
NaeI GCCGGC 1 cut(s) 621
NarI GGCGCC 2 cut(s) 75, 533
NciI CCSGG 5 cut(s) 192, 251, 280, 554, 663
NdeII GATC 5 cut(s) 242, 267, 424, 683, 853
NgoMIV GCCGGC 1 cut(s) 619
NlaIII CATG 1 cut(s) 811
NmuCI GTSAC 1 cut(s) 11
PaqCI CACCTGC 1 cut(s) 529
PauI GCGCGC 1 cut(s) 325
PciSI GCTCTTC 1 cut(s) 704
PcsI WCGNNNNNNNCGW 3 cut(s) 144, 243, 315
PdiI GCCGGC 1 cut(s) 621
Pfl23II CGTACG 1 cut(s) 687
PflFI GACNNNGTC 1 cut(s) 230
PkrI GCNGC 7 cut(s) 131, 335, 559, 598, 606, 660, 835
PleI GAGTC 1 cut(s) 478
PluTI GGCGCC 2 cut(s) 78, 536
PpsI GAGTC 1 cut(s) 478
PpuMI RGGWCCY 1 cut(s) 298
Psp5II RGGWCCY 1 cut(s) 298
PspEI GGTNACC 1 cut(s) 572
PspFI CCCAGC 1 cut(s) 772
PspLI CGTACG 1 cut(s) 687
PspPI GGNCC 6 cut(s) 84, 106, 194, 277, 298, 366
PspPPI RGGWCCY 1 cut(s) 298
PsuI RGATCY 2 cut(s) 242, 424
PsyI GACNNNGTC 1 cut(s) 230
PteI GCGCGC 1 cut(s) 325
PvuII CAGCTG 1 cut(s) 464
RsaI GTAC 5 cut(s) 590, 611, 689, 806, 860
RsaNI GTAC 5 cut(s) 589, 610, 688, 805, 859
SalI GTCGAC 3 cut(s) 134, 200, 224
SapI GCTCTTC 1 cut(s) 704
SaqAI TTAA 2 cut(s) 51, 387
SatI GCNGC 7 cut(s) 130, 334, 558, 597, 605, 659, 834
Sau3AI GATC 5 cut(s) 242, 267, 424, 683, 853
Sau96I GGNCC 6 cut(s) 84, 106, 194, 277, 298, 366
SchI GAGTC 1 cut(s) 478
ScrFI CCNGG 5 cut(s) 192, 251, 280, 554, 663
SduI GDGCHC 1 cut(s) 508
SfoI GGCGCC 2 cut(s) 76, 534
SgrDI CGTCGACG 2 cut(s) 134, 200
SinI GGWCC 2 cut(s) 106, 298
SmlI CTYRAG 2 cut(s) 50, 347
SmoI CTYRAG 2 cut(s) 50, 347
Sse9I AATT 1 cut(s) 819
SspDI GGCGCC 2 cut(s) 74, 532
SspMI CTAG 1 cut(s) 877
StyD4I CCNGG 5 cut(s) 190, 249, 278, 552, 661
TaaI ACNGT 3 cut(s) 449, 614, 632
TaiI ACGT 4 cut(s) 60, 225, 312, 387
TaqI TCGA 5 cut(s) 135, 201, 225, 303, 318
TasI AATT 1 cut(s) 819
TatI WGTACW 1 cut(s) 804
TauI GCSGC 4 cut(s) 132, 336, 560, 607
Tru1I TTAA 2 cut(s) 51, 387
Tru9I TTAA 2 cut(s) 51, 387
TseFI GTSAC 1 cut(s) 11
TseI GCWGC 3 cut(s) 596, 658, 833
Tsp45I GTSAC 1 cut(s) 11
TspGWI ACGGA 1 cut(s) 705
Tth111I GACNNNGTC 1 cut(s) 230
Vha464I CTTAAG 1 cut(s) 50
VpaK11BI GGWCC 2 cut(s) 106, 298
XmiI GTMKAC 3 cut(s) 135, 201, 225
XspI CTAG 1 cut(s) 877
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.