pycom01g21560

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0004046
Physical Location & Seq
Reverse (-)
19542949 .. 19543836
888 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g21560.1

Sequence Viewer

Length: 888 bp
ATGGCGTCCGGACACGAAGTCCAACTGAAGCTCACCTCCGCCCGCGACCTGAAGAACGTCAACTGGCGAAACGGCGCCTTGAAGCCCTACGCCGTCGTTTGGGTGGACCCCAATAACAAAAGCTCCACTCGCGTCGATGAGGAGGGCGACACGTCACCCTACTGGGACGAGACCCTCACCATCCACTTACCGGGCCCCGTTGACGGCGACACCACTCTCCACATCGACATCGTGCACGCCGGATCCGACCCGGATACCAAGCCCTTGATTGGGTCGGCCCGGCTCAAGCTCCGGGATGTCATCGATGACGTGGGCTTCGACGAGCCCACCAGCCGCACACTCCAGCTCAAGCGACCATCCGGTAGGCCCCAGGGGAAGGTTGACGTTAAGGTTACGATCAGGGAGCCGCGCTATCGTGCGCCGGAACCGTACAACGCTCCTCCTTATGGTGTCCCGCCGCCAGCTGGCTCTCGGGACTATACCGCTCCGCCAACGGCTTATGGGGCACCGTACGGCGTACCTGCACCTCCGCCGCCGCAAAATCCTTACTACGCGGCTCCTCCGCCTTCTGGGTACCCGTATAACCAATACAGTGCTGCTCCCGCGCCGCCGTACGGTCAGCCCGCTCCCTACGGTCAGATGGGACAAGGGAGTTACGGACGGCCGGGGCAGGGCTACGGACAGGGGGCGTACGGACAGGAGGAGAAGAAGAGCAAGTTTGGGGGGATGGGGACGGGACTGGCGGTAGGCGCGGTGGCTGGGGCGGTGGGTGGGGTGGCATTGGCGGAGGGGTTTGATTACGTGGAGGACAAAATTGCGGATGACGTGGCTGAGAAAGTGGAGGATGATGTGTACGACGGCGACGACGGCGACGGCGATGACTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

296

Amino Acids

31.4

Weight (kDa)

4.68

Isoelectric Point (pI)

35.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 6 - 102 8e-12 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 529
Acc65I GGTACC 1 cut(s) 573
AccB1I GGYRCC 3 cut(s) 74, 505, 573
AccBSI CCGCTC 2 cut(s) 485, 626
AccII CGCG 6 cut(s) 45, 132, 409, 554, 605, 752
AccIII TCCGGA 1 cut(s) 8
AclWI GGATC 2 cut(s) 237, 250
AcoI YGGCCR 1 cut(s) 662
AcuI CTGAAG 2 cut(s) 47, 71
AcyI GRCGYC 2 cut(s) 5, 75
AfaI GTAC 7 cut(s) 431, 512, 519, 575, 614, 692, 854
AflIII ACRYGT 1 cut(s) 150
AgsI TTSAA 1 cut(s) 82
AhdI GACNNNNNGTC 1 cut(s) 17
AjiI CACGTC 3 cut(s) 153, 310, 826
AjnI CCWGG 1 cut(s) 369
AluBI AGCT 5 cut(s) 31, 123, 289, 346, 464
AluI AGCT 5 cut(s) 31, 123, 289, 346, 464
Alw21I GWGCWC 1 cut(s) 237
Alw26I GTCTC 1 cut(s) 164
Alw44I GTGCAC 1 cut(s) 233
AlwI GGATC 2 cut(s) 237, 250
Ama87I CYCGRG 1 cut(s) 471
Aor13HI TCCGGA 1 cut(s) 8
AoxI GGCC 4 cut(s) 193, 276, 365, 662
ApaI GGGCCC 1 cut(s) 197
ApaLI GTGCAC 1 cut(s) 233
ApeKI GCWGC 1 cut(s) 596
ArsI GACNNNNNNTTYG 2 cut(s) 299, 331
Asp718I GGTACC 1 cut(s) 573
AspLEI GCGC 5 cut(s) 77, 411, 421, 607, 752
AspS9I GGNCC 5 cut(s) 106, 193, 194, 277, 366
AsuC2I CCSGG 5 cut(s) 192, 251, 280, 293, 666
AsuHPI GGTGA 3 cut(s) 25, 147, 169
AvaI CYCGRG 1 cut(s) 471
AvaII GGWCC 1 cut(s) 106
BaeGI GKGCMC 3 cut(s) 197, 237, 508
BamHI GGATCC 1 cut(s) 242
BanI GGYRCC 3 cut(s) 74, 505, 573
BanII GRGCYC 2 cut(s) 197, 327
Bbv12I GWGCWC 1 cut(s) 237
BbvI GCAGC 1 cut(s) 583
BccI CCATC 4 cut(s) 188, 364, 634, 721
BceAI ACGGC 9 cut(s) 77, 88, 220, 510, 529, 595, 677, 874, 883
BciT130I CCWGG 1 cut(s) 371
BciVI GTATCC 1 cut(s) 247
BcnI CCSGG 5 cut(s) 192, 251, 280, 293, 666
BcoDI GTCTC 1 cut(s) 164
BfaI CTAG 1 cut(s) 886
BfoI RGCGCY 1 cut(s) 78
BfuAI ACCTGC 1 cut(s) 529
BfuI GTATCC 1 cut(s) 247
BisI GCNGC 8 cut(s) 334, 407, 458, 533, 536, 555, 597, 608
BlsI GCNGC 8 cut(s) 335, 408, 459, 534, 537, 556, 598, 609
Bme1390I CCNGG 6 cut(s) 192, 251, 280, 293, 371, 666
Bme18I GGWCC 1 cut(s) 106
BmeRI GACNNNNNGTC 1 cut(s) 17
BmeT110I CYCGRG 1 cut(s) 471
BmgBI CACGTC 3 cut(s) 153, 310, 826
BmgT120I GGNCC 5 cut(s) 106, 193, 194, 277, 366
BmrFI CCNGG 6 cut(s) 192, 251, 280, 293, 371, 666
BmrI ACTGGG 1 cut(s) 172
BmuI ACTGGG 1 cut(s) 172
BpmI CTGGAG 1 cut(s) 326
BpuEI CTTGAG 2 cut(s) 269, 332
BpuMI CCSGG 5 cut(s) 192, 251, 280, 293, 666
Bsa29I ATCGAT 1 cut(s) 303
BsaAI YACGTR 1 cut(s) 802
BsaHI GRCGYC 2 cut(s) 5, 75
BsaI GGTCTC 1 cut(s) 164
BsaJI CCNNGG 3 cut(s) 369, 370, 665
BsaWI WCCGGW 2 cut(s) 8, 359
BsaXI ACNNNNNCTCC 2 cut(s) 107, 137
Bse1I ACTGG 3 cut(s) 68, 167, 744
BseAI TCCGGA 1 cut(s) 8
BseBI CCWGG 1 cut(s) 371
BseCI ATCGAT 1 cut(s) 303
BseDI CCNNGG 3 cut(s) 369, 370, 665
BseGI GGATG 6 cut(s) 180, 301, 356, 732, 826, 850
BseMII CTCAG 1 cut(s) 822
BseNI ACTGG 3 cut(s) 68, 167, 744
BseRI GAGGAG 4 cut(s) 155, 429, 549, 716
BseSI GKGCMC 3 cut(s) 197, 237, 508
BseX3I CGGCCG 1 cut(s) 662
BseXI GCAGC 1 cut(s) 583
BseYI CCCAGC 1 cut(s) 758
BsgI GTGCAG 1 cut(s) 507
Bsh1236I CGCG 6 cut(s) 45, 132, 409, 554, 605, 752
Bsh1285I CGRYCG 1 cut(s) 665
BshFI GGCC 4 cut(s) 195, 278, 367, 664
BshNI GGYRCC 3 cut(s) 74, 505, 573
BshVI ATCGAT 1 cut(s) 303
BsiEI CGRYCG 1 cut(s) 665
BsiHKAI GWGCWC 1 cut(s) 237
BsiHKCI CYCGRG 1 cut(s) 471
BsiSI CCGG 9 cut(s) 9, 191, 240, 251, 280, 292, 360, 422, 665
BsiWI CGTACG 3 cut(s) 510, 612, 690
BslFI GGGAC 6 cut(s) 179, 437, 488, 657, 745, 750
BsmAI GTCTC 1 cut(s) 164
BsmFI GGGAC 6 cut(s) 179, 437, 488, 657, 745, 750
BsnI GGCC 4 cut(s) 195, 278, 367, 664
Bso31I GGTCTC 1 cut(s) 164
BsoBI CYCGRG 1 cut(s) 471
Bsp120I GGGCCC 1 cut(s) 193
Bsp1286I GDGCHC 4 cut(s) 197, 237, 327, 508
Bsp13I TCCGGA 1 cut(s) 8
Bsp143I GATC 2 cut(s) 242, 396
BspANI GGCC 4 cut(s) 195, 278, 367, 664
BspCNI CTCAG 1 cut(s) 823
BspDI ATCGAT 1 cut(s) 303
BspEI TCCGGA 1 cut(s) 8
BspFNI CGCG 6 cut(s) 45, 132, 409, 554, 605, 752
BspMI ACCTGC 1 cut(s) 529
BspPI GGATC 2 cut(s) 237, 250
BspQI GCTCTTC 1 cut(s) 704
BspT107I GGYRCC 3 cut(s) 74, 505, 573
BspTNI GGTCTC 1 cut(s) 164
BsrBI CCGCTC 2 cut(s) 485, 626
BsrI ACTGG 3 cut(s) 68, 167, 744
BssECI CCNNGG 3 cut(s) 369, 370, 665
BssMI GATC 2 cut(s) 242, 396
BssNI GRCGYC 2 cut(s) 5, 75
Bst2UI CCWGG 1 cut(s) 371
Bst4CI ACNGT 5 cut(s) 429, 510, 593, 617, 635
Bst6I CTCTTC 1 cut(s) 704
BstACI GRCGYC 2 cut(s) 5, 75
BstBAI YACGTR 1 cut(s) 802
BstC8I GCNNGC 5 cut(s) 43, 237, 462, 466, 624
BstDEI CTNAG 1 cut(s) 831
BstF5I GGATG 6 cut(s) 180, 301, 356, 732, 826, 850
BstFNI CGCG 6 cut(s) 45, 132, 409, 554, 605, 752
BstH2I RGCGCY 1 cut(s) 78
BstHHI GCGC 5 cut(s) 77, 411, 421, 607, 752
BstKTI GATC 2 cut(s) 245, 399
BstMAI GTCTC 1 cut(s) 164
BstMBI GATC 2 cut(s) 242, 396
BstMCI CGRYCG 1 cut(s) 665
BstMWI GCNNNNNNNGC 5 cut(s) 129, 503, 602, 749, 867
BstNI CCWGG 1 cut(s) 371
BstSCI CCNGG 6 cut(s) 190, 249, 278, 291, 369, 664
BstSLI GKGCMC 3 cut(s) 197, 237, 508
BstUI CGCG 6 cut(s) 45, 132, 409, 554, 605, 752
BstV1I GCAGC 1 cut(s) 583
BstX2I RGATCY 1 cut(s) 242
BstYI RGATCY 1 cut(s) 242
BstZI CGGCCG 1 cut(s) 662
Bsu15I ATCGAT 1 cut(s) 303
BsuI GTATCC 1 cut(s) 247
BsuRI GGCC 4 cut(s) 195, 278, 367, 664
BsuTUI ATCGAT 1 cut(s) 303
BtrI CACGTC 3 cut(s) 153, 310, 826
BtsCI GGATG 6 cut(s) 180, 301, 356, 732, 826, 850
BtsIMutI CAGTG 1 cut(s) 598
BveI ACCTGC 1 cut(s) 529
Cac8I GCNNGC 5 cut(s) 43, 237, 462, 466, 624
CfoI GCGC 5 cut(s) 77, 411, 421, 607, 752
Cfr13I GGNCC 5 cut(s) 106, 193, 194, 277, 366
ClaI ATCGAT 1 cut(s) 303
CseI GACGC 1 cut(s) 121
Csp6I GTAC 7 cut(s) 430, 511, 518, 574, 613, 691, 853
CviQI GTAC 7 cut(s) 430, 511, 518, 574, 613, 691, 853
DdeI CTNAG 1 cut(s) 831
DinI GGCGCC 1 cut(s) 76
DpnI GATC 2 cut(s) 244, 398
DpnII GATC 2 cut(s) 242, 396
DriI GACNNNNNGTC 1 cut(s) 17
EaeI YGGCCR 1 cut(s) 662
EagI CGGCCG 1 cut(s) 662
Eam1104I CTCTTC 1 cut(s) 704
Eam1105I GACNNNNNGTC 1 cut(s) 17
EarI CTCTTC 1 cut(s) 704
EciI GGCGGA 5 cut(s) 28, 477, 519, 552, 800
EclXI CGGCCG 1 cut(s) 662
Eco24I GRGCYC 2 cut(s) 197, 327
Eco31I GGTCTC 1 cut(s) 164
Eco47I GGWCC 1 cut(s) 106
Eco52I CGGCCG 1 cut(s) 662
Eco57I CTGAAG 2 cut(s) 47, 71
Eco88I CYCGRG 1 cut(s) 471
EcoO109I RGGNCCY 2 cut(s) 194, 366
EcoRII CCWGG 1 cut(s) 369
EcoT38I GRGCYC 2 cut(s) 197, 327
EgeI GGCGCC 1 cut(s) 76
EheI GGCGCC 1 cut(s) 76
FaiI YATR 4 cut(s) 447, 480, 501, 582
FaqI GGGAC 6 cut(s) 179, 437, 488, 657, 745, 750
FauI CCCGC 4 cut(s) 50, 462, 610, 631
Fnu4HI GCNGC 8 cut(s) 334, 407, 458, 533, 536, 555, 597, 608
FokI GGATG 6 cut(s) 167, 308, 343, 739, 833, 857
FriOI GRGCYC 2 cut(s) 197, 327
Fsp4HI GCNGC 8 cut(s) 334, 407, 458, 533, 536, 555, 597, 608
FspBI CTAG 1 cut(s) 886
GlaI GCGC 5 cut(s) 76, 410, 420, 606, 751
GluI GCNGC 8 cut(s) 334, 407, 458, 533, 536, 555, 597, 608
GsaI CCCAGC 1 cut(s) 762
GsuI CTGGAG 1 cut(s) 326
HaeII RGCGCY 1 cut(s) 78
HaeIII GGCC 4 cut(s) 195, 278, 367, 664
HapII CCGG 9 cut(s) 9, 191, 240, 251, 280, 292, 360, 422, 665
HgaI GACGC 1 cut(s) 121
HhaI GCGC 5 cut(s) 77, 411, 421, 607, 752
Hin1I GRCGYC 2 cut(s) 5, 75
Hin6I GCGC 5 cut(s) 75, 409, 419, 605, 750
HinP1I GCGC 5 cut(s) 75, 409, 419, 605, 750
HincII GTYRAC 3 cut(s) 61, 202, 382
HindII GTYRAC 3 cut(s) 61, 202, 382
HpaII CCGG 9 cut(s) 9, 191, 240, 251, 280, 292, 360, 422, 665
HphI GGTGA 3 cut(s) 25, 147, 169
Hpy166II GTNNAC 6 cut(s) 61, 106, 202, 235, 382, 853
Hpy188I TCNGA 2 cut(s) 247, 639
Hpy188III TCNNGA 2 cut(s) 9, 473
Hpy8I GTNNAC 6 cut(s) 61, 106, 202, 235, 382, 853
Hpy99I CGWCG 7 cut(s) 98, 137, 323, 860, 866, 869, 875
HpyAV CCTTC 2 cut(s) 370, 576
HpyCH4III ACNGT 5 cut(s) 429, 510, 593, 617, 635
HpyCH4IV ACGT 6 cut(s) 57, 152, 309, 384, 801, 825
HpyCH4V TGCA 2 cut(s) 235, 524
HpyF10VI GCNNNNNNNGC 5 cut(s) 129, 503, 602, 749, 867
HpyF3I CTNAG 1 cut(s) 831
HpySE526I ACGT 6 cut(s) 57, 152, 309, 384, 801, 825
Hsp92I GRCGYC 2 cut(s) 5, 75
HspAI GCGC 5 cut(s) 75, 409, 419, 605, 750
KasI GGCGCC 1 cut(s) 74
Kpn2I TCCGGA 1 cut(s) 8
KpnI GGTACC 1 cut(s) 577
Kzo9I GATC 2 cut(s) 242, 396
LguI GCTCTTC 1 cut(s) 704
LmnI GCTCC 8 cut(s) 128, 294, 403, 442, 490, 562, 604, 631
Lsp1109I GCAGC 1 cut(s) 583
MaeI CTAG 1 cut(s) 886
MaeII ACGT 6 cut(s) 57, 152, 309, 384, 801, 825
MaeIII GTNAC 3 cut(s) 153, 391, 653
MalI GATC 2 cut(s) 244, 398
MbiI CCGCTC 2 cut(s) 485, 626
MboI GATC 2 cut(s) 242, 396
MboII GAAGA 3 cut(s) 64, 718, 721
MflI RGATCY 1 cut(s) 242
MhlI GDGCHC 4 cut(s) 197, 237, 327, 508
MluCI AATT 1 cut(s) 813
Mly113I GGCGCC 1 cut(s) 75
MmeI TCCRAC 2 cut(s) 46, 270
MroI TCCGGA 1 cut(s) 8
MseI TTAA 1 cut(s) 387
MspA1I CMGCKG 1 cut(s) 464
MspI CCGG 9 cut(s) 9, 191, 240, 251, 280, 292, 360, 422, 665
MspR9I CCNGG 6 cut(s) 192, 251, 280, 293, 371, 666
MvaI CCWGG 1 cut(s) 371
MvnI CGCG 6 cut(s) 45, 132, 409, 554, 605, 752
MwoI GCNNNNNNNGC 5 cut(s) 129, 503, 602, 749, 867
NarI GGCGCC 1 cut(s) 75
NciI CCSGG 5 cut(s) 192, 251, 280, 293, 666
NdeII GATC 2 cut(s) 242, 396
NmuCI GTSAC 1 cut(s) 153
PasI CCCWGGG 1 cut(s) 370
PciSI GCTCTTC 1 cut(s) 704
PcsI WCGNNNNNNNCGW 4 cut(s) 243, 315, 861, 873
Pfl23II CGTACG 3 cut(s) 510, 612, 690
PfoI TCCNGGA 1 cut(s) 291
PkrI GCNGC 8 cut(s) 335, 408, 459, 534, 537, 556, 598, 609
PluTI GGCGCC 1 cut(s) 78
Ppu21I YACGTR 1 cut(s) 802
Psp6I CCWGG 1 cut(s) 369
PspFI CCCAGC 1 cut(s) 758
PspGI CCWGG 1 cut(s) 369
PspLI CGTACG 3 cut(s) 510, 612, 690
PspOMI GGGCCC 1 cut(s) 193
PspPI GGNCC 5 cut(s) 106, 193, 194, 277, 366
PsuI RGATCY 1 cut(s) 242
PvuII CAGCTG 1 cut(s) 464
RsaI GTAC 7 cut(s) 431, 512, 519, 575, 614, 692, 854
RsaNI GTAC 7 cut(s) 430, 511, 518, 574, 613, 691, 853
SapI GCTCTTC 1 cut(s) 704
SaqAI TTAA 1 cut(s) 387
SatI GCNGC 8 cut(s) 334, 407, 458, 533, 536, 555, 597, 608
Sau3AI GATC 2 cut(s) 242, 396
Sau96I GGNCC 5 cut(s) 106, 193, 194, 277, 366
ScrFI CCNGG 6 cut(s) 192, 251, 280, 293, 371, 666
SduI GDGCHC 4 cut(s) 197, 237, 327, 508
SfoI GGCGCC 1 cut(s) 76
SinI GGWCC 1 cut(s) 106
SmlI CTYRAG 2 cut(s) 284, 347
SmoI CTYRAG 2 cut(s) 284, 347
Sse9I AATT 1 cut(s) 813
SspDI GGCGCC 1 cut(s) 74
SspMI CTAG 1 cut(s) 886
StyD4I CCNGG 6 cut(s) 190, 249, 278, 291, 369, 664
TaaI ACNGT 5 cut(s) 429, 510, 593, 617, 635
TaiI ACGT 6 cut(s) 60, 155, 312, 387, 804, 828
TaqI TCGA 4 cut(s) 135, 225, 303, 318
TasI AATT 1 cut(s) 813
TauI GCSGC 7 cut(s) 336, 409, 460, 535, 538, 557, 610
Tru1I TTAA 1 cut(s) 387
Tru9I TTAA 1 cut(s) 387
TscAI CASTG 1 cut(s) 598
TseFI GTSAC 1 cut(s) 153
TseI GCWGC 1 cut(s) 596
Tsp45I GTSAC 1 cut(s) 153
TspGWI ACGGA 3 cut(s) 672, 693, 708
TspRI CASTG 1 cut(s) 598
VneI GTGCAC 1 cut(s) 233
VpaK11BI GGWCC 1 cut(s) 106
XspI CTAG 1 cut(s) 886
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.