RLG00000019714

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0004046
Physical Location & Seq
Reverse (-)
55886817 .. 55887650
834 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019714

Sequence Viewer

Length: 834 bp
ATGGCGTCAGCCCACGAAGTCCAAGTGAAGCTCTCCTCCGCCAGAGACCTGAAGAACGTGAACTGGAGACACGGCCCAGTCAAGCCGTACGCCGTCGCGTGGGTCGAGCCGAGGAACAAGCTCTCAACCAAGGTGGACGAGGATGGCGACACGTCTCCCGAGTGGGACGAGACGCTGACAATCCCTATCCCCGGTCCTGTCAACGACGACACCATGCTTTACATCGACATCGTGCATGCCGGAGCCGAGCCCGACACCAAACAGCTGGTGGGATCTGCTCGGCTCCGGCTCCGCGACGTTGTCGATGACGTCGGGTATGACGAGCGCGCCAGCCACACTCTTCACCTCAAAAGGCCCTCCGGTAGGCCACAGGGAAAAGTGGACGTCAAGGTTACGGTGAGGGAGCCGCGCTATCATGCGCCGGAGCCCCATTATGGGCCGCCTTATGGGGTCCCACCGGCAGGCTCTGGAGGTTACCATGCCCCACCACCACCGCCGGTGTATGGCGACCCATATGCCGCACCACCACCGCCGCCGCCGGTATATGGTGGACCGTACCCTGCACCGCCATCCGGGTATCCCTACAATGCGACTCCGATAGCGTACAGTCAGCCAACTCAGCCGAGTTATGGTCAAGTGCAGTGCGGGCAGGAGGAGAAGAAGAAGAGCAAGTTTGGTGGGATGGGAACGGGATTGGCTGTGGGGGCGGTAGCGGGGGCGCTGGGTGCATTTGCGTTGGCTGAGGGTGTTGATTATGTGGAGGACAAAATTGCAGACGACGTGTCGGAGAAGGTGGTGGATAATCTGAGCTACTATGATGGCGATGACTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

278

Amino Acids

29.78

Weight (kDa)

5.15

Isoelectric Point (pI)

37.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 6 - 105 5.3e-11 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 312, 387
AccII CGCG 4 cut(s) 98, 294, 327, 409
AclWI GGATC 1 cut(s) 280
AcuI CTGAAG 1 cut(s) 71
AcyI GRCGYC 3 cut(s) 5, 309, 384
AfaI GTAC 3 cut(s) 89, 557, 605
AflIII ACRYGT 2 cut(s) 150, 780
AhdI GACNNNNNGTC 1 cut(s) 781
AjiI CACGTC 2 cut(s) 153, 781
AluBI AGCT 4 cut(s) 31, 121, 265, 810
AluI AGCT 4 cut(s) 31, 121, 265, 810
Alw26I GTCTC 4 cut(s) 39, 61, 159, 164
AlwI GGATC 1 cut(s) 280
AlwNI CAGNNNCTG 1 cut(s) 467
Ama87I CYCGRG 1 cut(s) 158
AoxI GGCC 4 cut(s) 73, 353, 365, 437
AspLEI GCGC 5 cut(s) 327, 329, 411, 421, 721
AspS9I GGNCC 6 cut(s) 74, 194, 354, 437, 451, 551
AsuC2I CCSGG 2 cut(s) 192, 574
AsuHPI GGTGA 2 cut(s) 335, 409
AvaI CYCGRG 1 cut(s) 158
AvaII GGWCC 3 cut(s) 194, 451, 551
BanII GRGCYC 2 cut(s) 252, 429
BbvCI CCTCAGC 1 cut(s) 741
BccI CCATC 4 cut(s) 137, 577, 676, 812
BceAI ACGGC 3 cut(s) 70, 77, 88
BcgI CGANNNNNNTGC 2 cut(s) 497, 531
BciVI GTATCC 1 cut(s) 588
BcnI CCSGG 2 cut(s) 192, 574
BcoDI GTCTC 4 cut(s) 39, 61, 159, 164
BfoI RGCGCY 1 cut(s) 722
BfuI GTATCC 1 cut(s) 588
BisI GCNGC 5 cut(s) 407, 440, 519, 533, 536
BlsI GCNGC 5 cut(s) 408, 441, 520, 534, 537
Bme1390I CCNGG 2 cut(s) 192, 574
Bme18I GGWCC 3 cut(s) 194, 451, 551
BmeRI GACNNNNNGTC 1 cut(s) 781
BmeT110I CYCGRG 1 cut(s) 158
BmgBI CACGTC 2 cut(s) 153, 781
BmgT120I GGNCC 6 cut(s) 74, 194, 354, 437, 451, 551
BmiI GGNNCC 7 cut(s) 244, 284, 290, 405, 426, 452, 453
BmrFI CCNGG 2 cut(s) 192, 574
BmrI ACTGGG 1 cut(s) 71
BmuI ACTGGG 1 cut(s) 71
BpmI CTGGAG 2 cut(s) 85, 489
Bpu10I CCTNAGC 1 cut(s) 741
BpuMI CCSGG 2 cut(s) 192, 574
BsaHI GRCGYC 3 cut(s) 5, 309, 384
BsaI GGTCTC 1 cut(s) 39
BsaJI CCNNGG 3 cut(s) 110, 129, 190
BsaWI WCCGGW 1 cut(s) 359
Bse118I RCCGGY 3 cut(s) 457, 496, 538
Bse1I ACTGG 2 cut(s) 68, 77
BseDI CCNNGG 3 cut(s) 110, 129, 190
BseGI GGATG 3 cut(s) 148, 569, 687
BseMII CTCAG 3 cut(s) 632, 732, 797
BseNI ACTGG 2 cut(s) 68, 77
BsePI GCGCGC 1 cut(s) 325
BseRI GAGGAG 2 cut(s) 25, 668
BseYI CCCAGC 1 cut(s) 721
BsgI GTGCAG 2 cut(s) 546, 659
Bsh1236I CGCG 4 cut(s) 98, 294, 327, 409
BshFI GGCC 4 cut(s) 75, 355, 367, 439
BsiHKCI CYCGRG 1 cut(s) 158
BsiSI CCGG 9 cut(s) 192, 240, 286, 360, 422, 458, 497, 539, 573
BsiWI CGTACG 1 cut(s) 87
BslFI GGGAC 2 cut(s) 179, 437
BsmAI GTCTC 4 cut(s) 39, 61, 159, 164
BsmBI CGTCTC 2 cut(s) 159, 164
BsmFI GGGAC 2 cut(s) 179, 437
BsnI GGCC 4 cut(s) 75, 355, 367, 439
Bso31I GGTCTC 1 cut(s) 39
BsoBI CYCGRG 1 cut(s) 158
Bsp1286I GDGCHC 2 cut(s) 252, 429
Bsp143I GATC 1 cut(s) 272
BspANI GGCC 4 cut(s) 75, 355, 367, 439
BspCNI CTCAG 3 cut(s) 631, 733, 798
BspFNI CGCG 4 cut(s) 98, 294, 327, 409
BspLI GGNNCC 7 cut(s) 244, 284, 290, 405, 426, 452, 453
BspPI GGATC 1 cut(s) 280
BspQI GCTCTTC 1 cut(s) 659
BspTNI GGTCTC 1 cut(s) 39
BsrFI RCCGGY 3 cut(s) 457, 496, 538
BsrI ACTGG 2 cut(s) 68, 77
BssAI RCCGGY 3 cut(s) 457, 496, 538
BssECI CCNNGG 3 cut(s) 110, 129, 190
BssHII GCGCGC 1 cut(s) 325
BssMI GATC 1 cut(s) 272
BssNI GRCGYC 3 cut(s) 5, 309, 384
BssT1I CCWWGG 1 cut(s) 129
Bst4CI ACNGT 3 cut(s) 397, 555, 608
Bst6I CTCTTC 2 cut(s) 345, 659
BstACI GRCGYC 3 cut(s) 5, 309, 384
BstC8I GCNNGC 5 cut(s) 237, 327, 331, 463, 647
BstDEI CTNAG 3 cut(s) 618, 741, 806
BstEII GGTNACC 1 cut(s) 473
BstENI CCTNNNNNAGG 1 cut(s) 361
BstF5I GGATG 3 cut(s) 148, 569, 687
BstFNI CGCG 4 cut(s) 98, 294, 327, 409
BstH2I RGCGCY 1 cut(s) 722
BstHHI GCGC 5 cut(s) 327, 329, 411, 421, 721
BstKTI GATC 1 cut(s) 275
BstMAI GTCTC 4 cut(s) 39, 61, 159, 164
BstMBI GATC 1 cut(s) 272
BstMWI GCNNNNNNNGC 4 cut(s) 619, 646, 704, 725
BstNSI RCATGY 1 cut(s) 239
BstPI GGTNACC 1 cut(s) 473
BstSCI CCNGG 2 cut(s) 190, 572
BstUI CGCG 4 cut(s) 98, 294, 327, 409
BstX2I RGATCY 1 cut(s) 272
BstXI CCANNNNNNTGG 1 cut(s) 265
BstYI RGATCY 1 cut(s) 272
BsuI GTATCC 1 cut(s) 588
BsuRI GGCC 4 cut(s) 75, 355, 367, 439
BtrI CACGTC 2 cut(s) 153, 781
BtsCI GGATG 3 cut(s) 148, 569, 687
BtsI GCAGTG 1 cut(s) 647
BtsIMutI CAGTG 1 cut(s) 647
Cac8I GCNNGC 5 cut(s) 237, 327, 331, 463, 647
CaiI CAGNNNCTG 1 cut(s) 467
CfoI GCGC 5 cut(s) 327, 329, 411, 421, 721
Cfr10I RCCGGY 3 cut(s) 457, 496, 538
Cfr13I GGNCC 6 cut(s) 74, 194, 354, 437, 451, 551
CseI GACGC 1 cut(s) 181
Csp6I GTAC 3 cut(s) 88, 556, 604
CspCI CAANNNNNGTGG 4 cut(s) 114, 149, 658, 693
CviAII CATG 4 cut(s) 214, 236, 416, 479
CviQI GTAC 3 cut(s) 88, 556, 604
DdeI CTNAG 3 cut(s) 618, 741, 806
DpnI GATC 1 cut(s) 274
DpnII GATC 1 cut(s) 272
DriI GACNNNNNGTC 1 cut(s) 781
Eam1104I CTCTTC 2 cut(s) 345, 659
Eam1105I GACNNNNNGTC 1 cut(s) 781
EarI CTCTTC 2 cut(s) 345, 659
EciI GGCGGA 1 cut(s) 28
Eco130I CCWWGG 1 cut(s) 129
Eco24I GRGCYC 2 cut(s) 252, 429
Eco31I GGTCTC 1 cut(s) 39
Eco47I GGWCC 3 cut(s) 194, 451, 551
Eco57I CTGAAG 1 cut(s) 71
Eco88I CYCGRG 1 cut(s) 158
Eco91I GGTNACC 1 cut(s) 473
EcoNI CCTNNNNNAGG 1 cut(s) 361
EcoO109I RGGNCCY 2 cut(s) 354, 451
EcoO65I GGTNACC 1 cut(s) 473
EcoT14I CCWWGG 1 cut(s) 129
EcoT38I GRGCYC 2 cut(s) 252, 429
ErhI CCWWGG 1 cut(s) 129
Esp3I CGTCTC 2 cut(s) 159, 164
FaeI CATG 4 cut(s) 217, 239, 419, 482
FaqI GGGAC 2 cut(s) 179, 437
FatI CATG 4 cut(s) 213, 235, 415, 478
FauI CCCGC 2 cut(s) 638, 706
FauNDI CATATG 1 cut(s) 514
Fnu4HI GCNGC 5 cut(s) 407, 440, 519, 533, 536
FokI GGATG 3 cut(s) 155, 556, 694
FriOI GRGCYC 2 cut(s) 252, 429
Fsp4HI GCNGC 5 cut(s) 407, 440, 519, 533, 536
GlaI GCGC 5 cut(s) 326, 328, 410, 420, 720
GluI GCNGC 5 cut(s) 407, 440, 519, 533, 536
GsaI CCCAGC 1 cut(s) 725
GsuI CTGGAG 2 cut(s) 85, 489
HaeII RGCGCY 1 cut(s) 722
HaeIII GGCC 4 cut(s) 75, 355, 367, 439
HapII CCGG 9 cut(s) 192, 240, 286, 360, 422, 458, 497, 539, 573
HgaI GACGC 1 cut(s) 181
HhaI GCGC 5 cut(s) 327, 329, 411, 421, 721
Hin1I GRCGYC 3 cut(s) 5, 309, 384
Hin1II CATG 4 cut(s) 217, 239, 419, 482
Hin6I GCGC 5 cut(s) 325, 327, 409, 419, 719
HinP1I GCGC 5 cut(s) 325, 327, 409, 419, 719
HincII GTYRAC 1 cut(s) 202
HindII GTYRAC 1 cut(s) 202
HinfI GANTC 1 cut(s) 592
HpaII CCGG 9 cut(s) 192, 240, 286, 360, 422, 458, 497, 539, 573
HphI GGTGA 2 cut(s) 335, 409
Hpy166II GTNNAC 5 cut(s) 61, 136, 202, 382, 551
Hpy188I TCNGA 3 cut(s) 597, 787, 807
Hpy188III TCNNGA 2 cut(s) 158, 468
Hpy8I GTNNAC 5 cut(s) 61, 136, 202, 382, 551
Hpy99I CGWCG 5 cut(s) 98, 209, 299, 314, 782
HpyAV CCTTC 1 cut(s) 784
HpyCH4III ACNGT 3 cut(s) 397, 555, 608
HpyCH4IV ACGT 6 cut(s) 57, 152, 297, 309, 384, 780
HpyCH4V TGCA 5 cut(s) 235, 563, 640, 728, 773
HpyF10VI GCNNNNNNNGC 4 cut(s) 619, 646, 704, 725
HpyF3I CTNAG 3 cut(s) 618, 741, 806
HpySE526I ACGT 6 cut(s) 57, 152, 297, 309, 384, 780
Hsp92I GRCGYC 3 cut(s) 5, 309, 384
Hsp92II CATG 4 cut(s) 217, 239, 419, 482
HspAI GCGC 5 cut(s) 325, 327, 409, 419, 719
KflI GGGWCCC 1 cut(s) 451
Kzo9I GATC 1 cut(s) 272
LguI GCTCTTC 1 cut(s) 659
LmnI GCTCC 5 cut(s) 242, 288, 294, 403, 424
MaeII ACGT 6 cut(s) 57, 152, 297, 309, 384, 780
MaeIII GTNAC 2 cut(s) 391, 473
MalI GATC 1 cut(s) 274
MboI GATC 1 cut(s) 272
MboII GAAGA 5 cut(s) 64, 332, 670, 673, 676
MflI RGATCY 1 cut(s) 272
MhlI GDGCHC 2 cut(s) 252, 429
MluCI AATT 1 cut(s) 768
MlyI GAGTC 1 cut(s) 586
MmeI TCCRAC 1 cut(s) 765
MspA1I CMGCKG 1 cut(s) 265
MspI CCGG 9 cut(s) 192, 240, 286, 360, 422, 458, 497, 539, 573
MspR9I CCNGG 2 cut(s) 192, 574
MvnI CGCG 4 cut(s) 98, 294, 327, 409
MwoI GCNNNNNNNGC 4 cut(s) 619, 646, 704, 725
NciI CCSGG 2 cut(s) 192, 574
NdeI CATATG 1 cut(s) 514
NdeII GATC 1 cut(s) 272
NlaIII CATG 4 cut(s) 217, 239, 419, 482
NlaIV GGNNCC 7 cut(s) 244, 284, 290, 405, 426, 452, 453
NmeAIII GCCGAG 4 cut(s) 135, 259, 271, 648
NspI RCATGY 1 cut(s) 239
PaeI GCATGC 1 cut(s) 239
PauI GCGCGC 1 cut(s) 325
PciSI GCTCTTC 1 cut(s) 659
PcsI WCGNNNNNNNCGW 3 cut(s) 102, 144, 318
Pfl23II CGTACG 1 cut(s) 87
PflFI GACNNNGTC 1 cut(s) 299
PkrI GCNGC 5 cut(s) 408, 441, 520, 534, 537
PleI GAGTC 1 cut(s) 586
PpsI GAGTC 1 cut(s) 586
PpuMI RGGWCCY 1 cut(s) 451
Psp5II RGGWCCY 1 cut(s) 451
PspEI GGTNACC 1 cut(s) 473
PspFI CCCAGC 1 cut(s) 721
PspLI CGTACG 1 cut(s) 87
PspN4I GGNNCC 7 cut(s) 244, 284, 290, 405, 426, 452, 453
PspPI GGNCC 6 cut(s) 74, 194, 354, 437, 451, 551
PspPPI RGGWCCY 1 cut(s) 451
PstNI CAGNNNCTG 1 cut(s) 467
PsuI RGATCY 1 cut(s) 272
PsyI GACNNNGTC 1 cut(s) 299
PteI GCGCGC 1 cut(s) 325
PvuII CAGCTG 1 cut(s) 265
RsaI GTAC 3 cut(s) 89, 557, 605
RsaNI GTAC 3 cut(s) 88, 556, 604
SapI GCTCTTC 1 cut(s) 659
SatI GCNGC 5 cut(s) 407, 440, 519, 533, 536
Sau3AI GATC 1 cut(s) 272
Sau96I GGNCC 6 cut(s) 74, 194, 354, 437, 451, 551
SchI GAGTC 1 cut(s) 586
ScrFI CCNGG 2 cut(s) 192, 574
SduI GDGCHC 2 cut(s) 252, 429
SgrAI CRCCGGYG 1 cut(s) 496
SinI GGWCC 3 cut(s) 194, 451, 551
SphI GCATGC 1 cut(s) 239
Sse9I AATT 1 cut(s) 768
StyD4I CCNGG 2 cut(s) 190, 572
StyI CCWWGG 1 cut(s) 129
TaaI ACNGT 3 cut(s) 397, 555, 608
TaiI ACGT 6 cut(s) 60, 155, 300, 312, 387, 783
TaqI TCGA 3 cut(s) 105, 225, 303
TasI AATT 1 cut(s) 768
TauI GCSGC 5 cut(s) 409, 442, 521, 535, 538
TscAI CASTG 1 cut(s) 647
TspRI CASTG 1 cut(s) 647
Tth111I GACNNNGTC 1 cut(s) 299
VpaK11BI GGWCC 3 cut(s) 194, 451, 551
XagI CCTNNNNNAGG 1 cut(s) 361
XceI RCATGY 1 cut(s) 239
XcmI CCANNNNNNNNNTGG 1 cut(s) 265
ZraI GACGTC 2 cut(s) 310, 385
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.