RLG00000015214

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0004046
Physical Location & Seq
Reverse (-)
63209120 .. 63209935
816 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015214

Sequence Viewer

Length: 816 bp
ATGGCTTCCGGCTACGAAGTAGAGGTCACAATTTCTTCTGCCAAAGACCTCAAAAACGTGAATTGGCGTCACGGAGAGCTGAAGCCCTATGCTGTGGTTTGGGTAGACTCAAACAACAAATGCTCCACCCACGTGGACGAATACGGCGACGACTCGCCCACGTGGGACGAGACGCTCTCAATCCCGTTACACGGTCCAATCGAGGACTCGACCCTCTTTGTGGATATCATCCATGTTAAGGCGGAAGAGGACGTCAAGCCATTGATCGGGTCCGCGAAACTCCCGCTGAGTGAGGTCCTCGATGACGTGGGACTCGGGGCGTTGTCCGTTCACAAGTTGAAGCTCAAGCGCCCCTCAGGTAGGCCTCACGGCAAGTTGGAAGTCAAAGTCACGGTCCGCCCGCCCCGTTACCAAGCACCAGACCCGTATCGTGCTCCCGCGGCCTACGGGTACGGAGGCCCACCGCCGCAGTCGCAGTCTAGAGACTACGGCGCACCTCAGCAGTATAATTACCCATATGCCCCTCCCGCCCAACCCCAGTACTACCAAAGCAGTGGCGTCCCACAATCAGGGTACGGTTACAATCAGCCATCTTACGATCATGGTCAGTATGGGAGTGGCGGGTCGTCGGAGAAGCCGAAGGAGAAGAGCAAGTATGGGATGGGGACCGGATTAGCTGTGGGCGCGGTAGCCGGAGTGCTGGGTGGTCTTGCGATTGCTGAAGGAGCGGATTACGTGGAGGACAAGATTGCTGAGGACGTAGCAGAGAAAGTTGAGGAGGATCTTGGGTACGATGATTTTGGCGACGATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

272

Amino Acids

29.31

Weight (kDa)

4.8

Isoelectric Point (pI)

39.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 7 - 100 4.3e-11 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 255
AccBSI CCGCTC 1 cut(s) 728
AccI GTMKAC 1 cut(s) 105
AccII CGCG 3 cut(s) 275, 440, 686
AclWI GGATC 1 cut(s) 789
AcuI CTGAAG 2 cut(s) 101, 741
AcvI CACGTG 2 cut(s) 133, 162
AcyI GRCGYC 3 cut(s) 67, 252, 558
AfaI GTAC 4 cut(s) 452, 542, 575, 791
AfiI CCNNNNNNNGG 6 cut(s) 191, 220, 238, 266, 360, 569
AgsI TTSAA 1 cut(s) 340
AjiI CACGTC 1 cut(s) 307
AleI CACNNNNGTG 1 cut(s) 131
AluBI AGCT 3 cut(s) 79, 343, 677
AluI AGCT 3 cut(s) 79, 343, 677
Alw21I GWGCWC 1 cut(s) 436
Alw26I GTCTC 2 cut(s) 164, 477
AlwI GGATC 1 cut(s) 789
Ama87I CYCGRG 1 cut(s) 314
AoxI GGCC 3 cut(s) 362, 441, 457
ArsI GACNNNNNNTTYG 4 cut(s) 9, 41, 378, 410
AspLEI GCGC 3 cut(s) 351, 494, 686
AspS9I GGNCC 6 cut(s) 194, 270, 295, 394, 458, 666
AvaI CYCGRG 1 cut(s) 314
AvaII GGWCC 5 cut(s) 194, 270, 295, 394, 666
AxyI CCTNAGG 1 cut(s) 355
BbrPI CACGTG 2 cut(s) 133, 162
Bbv12I GWGCWC 1 cut(s) 436
BbvCI CCTCAGC 2 cut(s) 498, 753
BccI CCATC 2 cut(s) 598, 655
BceAI ACGGC 3 cut(s) 160, 385, 505
BcoDI GTCTC 2 cut(s) 164, 477
BfaI CTAG 1 cut(s) 480
BfoI RGCGCY 1 cut(s) 352
BisI GCNGC 2 cut(s) 441, 467
BlsI GCNGC 2 cut(s) 442, 468
BmcAI AGTACT 1 cut(s) 542
Bme18I GGWCC 5 cut(s) 194, 270, 295, 394, 666
BmeT110I CYCGRG 1 cut(s) 314
BmgBI CACGTC 1 cut(s) 307
BmgT120I GGNCC 6 cut(s) 194, 270, 295, 394, 458, 666
BmiI GGNNCC 2 cut(s) 271, 667
BmrI ACTGGG 1 cut(s) 532
BmuI ACTGGG 1 cut(s) 532
BplI GAGNNNNNCTC 2 cut(s) 161, 193
Bpu10I CCTNAGC 2 cut(s) 498, 753
BpuEI CTTGAG 1 cut(s) 329
BsaAI YACGTR 3 cut(s) 133, 162, 736
BsaHI GRCGYC 3 cut(s) 67, 252, 558
BsaJI CCNNGG 1 cut(s) 438
BsaWI WCCGGW 1 cut(s) 668
BsaXI ACNNNNNCTCC 2 cut(s) 107, 137
Bsc4I CCNNNNNNNGG 6 cut(s) 191, 220, 238, 266, 360, 569
Bse1I ACTGG 1 cut(s) 538
Bse21I CCTNAGG 1 cut(s) 355
BseDI CCNNGG 1 cut(s) 438
BseGI GGATG 2 cut(s) 228, 666
BseLI CCNNNNNNNGG 6 cut(s) 191, 220, 238, 266, 360, 569
BseMII CTCAG 4 cut(s) 278, 369, 512, 744
BseNI ACTGG 1 cut(s) 538
BseRI GAGGAG 1 cut(s) 791
BseYI CCCAGC 1 cut(s) 700
Bsh1236I CGCG 3 cut(s) 275, 440, 686
BshFI GGCC 3 cut(s) 364, 443, 459
BsiHKAI GWGCWC 1 cut(s) 436
BsiHKCI CYCGRG 1 cut(s) 314
BsiSI CCGG 3 cut(s) 9, 669, 693
BslFI GGGAC 4 cut(s) 179, 324, 545, 679
BslI CCNNNNNNNGG 6 cut(s) 191, 220, 238, 266, 360, 569
BsmAI GTCTC 2 cut(s) 164, 477
BsmBI CGTCTC 1 cut(s) 164
BsmFI GGGAC 4 cut(s) 179, 324, 545, 679
BsnI GGCC 3 cut(s) 364, 443, 459
BsoBI CYCGRG 1 cut(s) 314
Bsp1286I GDGCHC 1 cut(s) 436
Bsp143I GATC 3 cut(s) 264, 598, 781
BspANI GGCC 3 cut(s) 364, 443, 459
BspCNI CTCAG 4 cut(s) 279, 368, 511, 745
BspFNI CGCG 3 cut(s) 275, 440, 686
BspLI GGNNCC 2 cut(s) 271, 667
BspPI GGATC 1 cut(s) 789
BspQI GCTCTTC 1 cut(s) 641
BsrBI CCGCTC 1 cut(s) 728
BsrI ACTGG 1 cut(s) 538
BssECI CCNNGG 1 cut(s) 438
BssMI GATC 3 cut(s) 264, 598, 781
BssNI GRCGYC 3 cut(s) 67, 252, 558
Bst4CI ACNGT 3 cut(s) 194, 394, 578
Bst6I CTCTTC 2 cut(s) 240, 641
BstACI GRCGYC 3 cut(s) 67, 252, 558
BstBAI YACGTR 3 cut(s) 133, 162, 736
BstC8I GCNNGC 1 cut(s) 401
BstDEI CTNAG 4 cut(s) 287, 355, 498, 753
BstDSI CCRYGG 1 cut(s) 438
BstENI CCTNNNNNAGG 1 cut(s) 358
BstF5I GGATG 2 cut(s) 228, 666
BstFNI CGCG 3 cut(s) 275, 440, 686
BstH2I RGCGCY 1 cut(s) 352
BstHHI GCGC 3 cut(s) 351, 494, 686
BstKTI GATC 3 cut(s) 267, 601, 784
BstMAI GTCTC 2 cut(s) 164, 477
BstMBI GATC 3 cut(s) 264, 598, 781
BstMWI GCNNNNNNNGC 5 cut(s) 440, 472, 527, 683, 725
BstUI CGCG 3 cut(s) 275, 440, 686
BstX2I RGATCY 1 cut(s) 781
BstXI CCANNNNNNTGG 2 cut(s) 133, 554
BstYI RGATCY 1 cut(s) 781
Bsu36I CCTNAGG 1 cut(s) 355
BsuRI GGCC 3 cut(s) 364, 443, 459
BtgI CCRYGG 1 cut(s) 438
BtrI CACGTC 1 cut(s) 307
BtsCI GGATG 2 cut(s) 228, 666
BtsI GCAGTG 1 cut(s) 559
BtsIMutI CAGTG 1 cut(s) 559
Cac8I GCNNGC 1 cut(s) 401
CfoI GCGC 3 cut(s) 351, 494, 686
Cfr13I GGNCC 6 cut(s) 194, 270, 295, 394, 458, 666
Cfr42I CCGCGG 1 cut(s) 441
CpoI CGGWCCG 1 cut(s) 394
CseI GACGC 3 cut(s) 56, 181, 547
Csp6I GTAC 4 cut(s) 451, 541, 574, 790
CspI CGGWCCG 1 cut(s) 394
CviAII CATG 2 cut(s) 233, 602
CviQI GTAC 4 cut(s) 451, 541, 574, 790
DdeI CTNAG 4 cut(s) 287, 355, 498, 753
DpnI GATC 3 cut(s) 266, 600, 783
DpnII GATC 3 cut(s) 264, 598, 781
Eam1104I CTCTTC 2 cut(s) 240, 641
EarI CTCTTC 2 cut(s) 240, 641
EciI GGCGGA 2 cut(s) 257, 386
Eco147I AGGCCT 1 cut(s) 364
Eco32I GATATC 1 cut(s) 226
Eco47I GGWCC 5 cut(s) 194, 270, 295, 394, 666
Eco57I CTGAAG 2 cut(s) 101, 741
Eco72I CACGTG 2 cut(s) 133, 162
Eco81I CCTNAGG 1 cut(s) 355
Eco88I CYCGRG 1 cut(s) 314
EcoNI CCTNNNNNAGG 1 cut(s) 358
EcoO109I RGGNCCY 1 cut(s) 295
EcoRV GATATC 1 cut(s) 226
Esp3I CGTCTC 1 cut(s) 164
FaeI CATG 2 cut(s) 236, 605
FaiI YATR 8 cut(s) 90, 234, 507, 517, 519, 603, 612, 657
FaqI GGGAC 4 cut(s) 179, 324, 545, 679
FatI CATG 2 cut(s) 232, 601
FauI CCCGC 5 cut(s) 291, 408, 445, 535, 614
FauNDI CATATG 1 cut(s) 517
FblI GTMKAC 1 cut(s) 105
Fnu4HI GCNGC 2 cut(s) 441, 467
FokI GGATG 2 cut(s) 215, 673
Fsp4HI GCNGC 2 cut(s) 441, 467
FspBI CTAG 1 cut(s) 480
GlaI GCGC 3 cut(s) 350, 493, 685
GluI GCNGC 2 cut(s) 441, 467
GsaI CCCAGC 1 cut(s) 704
HaeII RGCGCY 1 cut(s) 352
HaeIII GGCC 3 cut(s) 364, 443, 459
HapII CCGG 3 cut(s) 9, 669, 693
HgaI GACGC 3 cut(s) 56, 181, 547
HhaI GCGC 3 cut(s) 351, 494, 686
Hin1I GRCGYC 3 cut(s) 67, 252, 558
Hin1II CATG 2 cut(s) 236, 605
Hin6I GCGC 3 cut(s) 349, 492, 684
HinP1I GCGC 3 cut(s) 349, 492, 684
HinfI GANTC 4 cut(s) 107, 152, 206, 312
HpaII CCGG 3 cut(s) 9, 669, 693
Hpy166II GTNNAC 3 cut(s) 106, 136, 331
Hpy188I TCNGA 1 cut(s) 631
Hpy188III TCNNGA 1 cut(s) 480
Hpy8I GTNNAC 3 cut(s) 106, 136, 331
Hpy99I CGWCG 3 cut(s) 152, 631, 809
HpyAV CCTTC 2 cut(s) 634, 716
HpyCH4III ACNGT 3 cut(s) 194, 394, 578
HpyCH4IV ACGT 7 cut(s) 57, 132, 161, 252, 306, 735, 759
HpyF10VI GCNNNNNNNGC 5 cut(s) 440, 472, 527, 683, 725
HpyF3I CTNAG 4 cut(s) 287, 355, 498, 753
HpySE526I ACGT 7 cut(s) 57, 132, 161, 252, 306, 735, 759
Hsp92I GRCGYC 3 cut(s) 67, 252, 558
Hsp92II CATG 2 cut(s) 236, 605
HspAI GCGC 3 cut(s) 349, 492, 684
KspI CCGCGG 1 cut(s) 441
Kzo9I GATC 3 cut(s) 264, 598, 781
LguI GCTCTTC 1 cut(s) 641
LmnI GCTCC 3 cut(s) 128, 439, 725
LpnPI CCDG 8 cut(s) 22, 342, 432, 551, 555, 682, 686, 706
MaeI CTAG 1 cut(s) 480
MaeII ACGT 7 cut(s) 57, 132, 161, 252, 306, 735, 759
MaeIII GTNAC 6 cut(s) 25, 68, 186, 388, 407, 578
MalI GATC 3 cut(s) 266, 600, 783
MbiI CCGCTC 1 cut(s) 728
MboI GATC 3 cut(s) 264, 598, 781
MboII GAAGA 3 cut(s) 27, 257, 658
MflI RGATCY 1 cut(s) 781
MhlI GDGCHC 1 cut(s) 436
MluCI AATT 3 cut(s) 30, 61, 508
MlyI GAGTC 4 cut(s) 101, 146, 200, 306
MmeI TCCRAC 2 cut(s) 357, 609
MseI TTAA 1 cut(s) 237
MslI CAYNNNNRTG 1 cut(s) 131
MspA1I CMGCKG 2 cut(s) 286, 440
MspI CCGG 3 cut(s) 9, 669, 693
MvnI CGCG 3 cut(s) 275, 440, 686
MwoI GCNNNNNNNGC 5 cut(s) 440, 472, 527, 683, 725
NdeI CATATG 1 cut(s) 517
NdeII GATC 3 cut(s) 264, 598, 781
NlaIII CATG 2 cut(s) 236, 605
NlaIV GGNNCC 2 cut(s) 271, 667
NmuCI GTSAC 3 cut(s) 25, 68, 388
OliI CACNNNNGTG 1 cut(s) 131
PceI AGGCCT 1 cut(s) 364
PciSI GCTCTTC 1 cut(s) 641
PcsI WCGNNNNNNNCGW 3 cut(s) 144, 198, 635
PkrI GCNGC 2 cut(s) 442, 468
PleI GAGTC 4 cut(s) 101, 146, 200, 306
PmaCI CACGTG 2 cut(s) 133, 162
PmlI CACGTG 2 cut(s) 133, 162
PpsI GAGTC 4 cut(s) 101, 146, 200, 306
Ppu21I YACGTR 3 cut(s) 133, 162, 736
PpuMI RGGWCCY 1 cut(s) 295
Psp5II RGGWCCY 1 cut(s) 295
PspCI CACGTG 2 cut(s) 133, 162
PspFI CCCAGC 1 cut(s) 700
PspN4I GGNNCC 2 cut(s) 271, 667
PspPI GGNCC 6 cut(s) 194, 270, 295, 394, 458, 666
PspPPI RGGWCCY 1 cut(s) 295
PsuI RGATCY 1 cut(s) 781
RsaI GTAC 4 cut(s) 452, 542, 575, 791
RsaNI GTAC 4 cut(s) 451, 541, 574, 790
RseI CAYNNNNRTG 1 cut(s) 131
Rsr2I CGGWCCG 1 cut(s) 394
RsrII CGGWCCG 1 cut(s) 394
SacII CCGCGG 1 cut(s) 441
SapI GCTCTTC 1 cut(s) 641
SaqAI TTAA 1 cut(s) 237
SatI GCNGC 2 cut(s) 441, 467
Sau3AI GATC 3 cut(s) 264, 598, 781
Sau96I GGNCC 6 cut(s) 194, 270, 295, 394, 458, 666
ScaI AGTACT 1 cut(s) 542
SchI GAGTC 4 cut(s) 101, 146, 200, 306
SduI GDGCHC 1 cut(s) 436
Sfr303I CCGCGG 1 cut(s) 441
SgrBI CCGCGG 1 cut(s) 441
SinI GGWCC 5 cut(s) 194, 270, 295, 394, 666
SmiMI CAYNNNNRTG 1 cut(s) 131
SmlI CTYRAG 1 cut(s) 344
SmoI CTYRAG 1 cut(s) 344
Sse9I AATT 3 cut(s) 30, 61, 508
SseBI AGGCCT 1 cut(s) 364
SspMI CTAG 1 cut(s) 480
StuI AGGCCT 1 cut(s) 364
TaaI ACNGT 3 cut(s) 194, 394, 578
TaiI ACGT 7 cut(s) 60, 135, 164, 255, 309, 738, 762
TaqI TCGA 3 cut(s) 201, 209, 300
TasI AATT 3 cut(s) 30, 61, 508
TatI WGTACW 1 cut(s) 540
TauI GCSGC 2 cut(s) 443, 469
Tru1I TTAA 1 cut(s) 237
Tru9I TTAA 1 cut(s) 237
TscAI CASTG 1 cut(s) 559
TseFI GTSAC 3 cut(s) 25, 68, 388
Tsp45I GTSAC 3 cut(s) 25, 68, 388
TspGWI ACGGA 3 cut(s) 87, 316, 468
TspRI CASTG 1 cut(s) 559
VpaK11BI GGWCC 5 cut(s) 194, 270, 295, 394, 666
XagI CCTNNNNNAGG 1 cut(s) 358
XbaI TCTAGA 1 cut(s) 479
XmiI GTMKAC 1 cut(s) 105
XspI CTAG 1 cut(s) 480
ZraI GACGTC 1 cut(s) 253
ZrmI AGTACT 1 cut(s) 542
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.