Rw6G003310

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0004046
Physical Location & Seq
Forward (+)
5259720 .. 5261186
1467 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G003310.1

Sequence Viewer

Length: 825 bp
ATGGCTTCCGGCTACGAAGTAGAGGTCACAATTTCTTCCGCCAAAGACCTCAAAAACGTGAATTGGCGCCACGGAGAGCTGAAGCCCTACGCTGTTGTTTGGGTGGATCCAAACAATAAATGCTCCACCCACGTGGACGAATACGGCGACGACTCGCCCACATGGGATGAGACGCTCTCGATCCCGTTACGGGGTCCCGTCGAGGACTCGACCCTTTTCGTGGACATCATCCACGTCAAGGCGGACGAGGACGTCAAGCCGTTGATCGGATCCGCAAAACTCCCGCTTAGGGAGGTCCTCGATGACGTGGGACTCGGAGCACCGTCCGTTCACAAGTTGAAGCTCAAGCGCCCCTCGGGTAGGCCCCACGGGAAGTTGGAAGTCAAAGTCACAGTTCGCCCGCCCCGTTACCAAGCACCTGACCCGTATCACGCCCCCGCGCCCTACGGGTATGGGATCGCACCGTCGCAGTCGAGAGACTACGGCGCACCTCAGCAGTATAATTACCAATATGCCCCTCCTGCCCAACCCCAATATTACCAAAGCAGCAGCGTCCCACAAGCAGGGTACGGTTACAATCAGCCATCTTACGATCAGAGTCAGTATGGGAGTGGCGGGTCGTACGGGTACGGGTCGTCGGAGAAGCCGAAGGAGAAGAGCAAGTTTGGGGTCGGGACAGGATTGGCTGTGGGCGCCGTAGCCGGAGTGTTGGGTGGACTTGCGATTGCTGAAGGAGTGGATTACGTGGAGGACAAGATTGCCGATGATGCAGCGGAGAAAGTGGAGGAGGATCTTGGCTATGATGATTTTGGCGATGATGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

274

Amino Acids

29.7

Weight (kDa)

4.78

Isoelectric Point (pI)

36.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 7 - 100 1.5e-10 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 251
AatII GACGTC 1 cut(s) 255
AccB1I GGYRCC 2 cut(s) 66, 692
AccII CGCG 1 cut(s) 440
AciI CCGC 8 cut(s) 39, 242, 273, 284, 401, 438, 615, 773
AclWI GGATC 7 cut(s) 101, 114, 175, 264, 277, 464, 798
AcuI CTGAAG 2 cut(s) 101, 750
AcvI CACGTG 1 cut(s) 133
AcyI GRCGYC 3 cut(s) 67, 252, 693
AfaI GTAC 3 cut(s) 569, 623, 629
AfiI CCNNNNNNNGG 8 cut(s) 190, 191, 220, 238, 266, 289, 360, 563
AgsI TTSAA 1 cut(s) 340
AjiI CACGTC 2 cut(s) 235, 307
AleI CACNNNNGTG 1 cut(s) 131
AluBI AGCT 2 cut(s) 79, 343
AluI AGCT 2 cut(s) 79, 343
Alw21I GWGCWC 1 cut(s) 322
Alw26I GTCTC 2 cut(s) 164, 471
AlwI GGATC 7 cut(s) 101, 114, 175, 264, 277, 464, 798
Ama87I CYCGRG 1 cut(s) 355
AoxI GGCC 1 cut(s) 362
ApeKI GCWGC 3 cut(s) 546, 549, 770
ArsI GACNNNNNNTTYG 2 cut(s) 9, 41
AspLEI GCGC 5 cut(s) 69, 351, 442, 488, 695
AspS9I GGNCC 3 cut(s) 194, 295, 363
AvaI CYCGRG 1 cut(s) 355
AvaII GGWCC 2 cut(s) 194, 295
BamHI GGATCC 2 cut(s) 106, 269
BanI GGYRCC 2 cut(s) 66, 692
BbrPI CACGTG 1 cut(s) 133
Bbv12I GWGCWC 1 cut(s) 322
BbvCI CCTCAGC 1 cut(s) 492
BbvI GCAGC 3 cut(s) 558, 561, 782
BccI CCATC 1 cut(s) 592
BceAI ACGGC 4 cut(s) 160, 244, 499, 680
BcoDI GTCTC 2 cut(s) 164, 471
BfoI RGCGCY 3 cut(s) 70, 352, 696
BisI GCNGC 3 cut(s) 547, 550, 771
BlsI GCNGC 3 cut(s) 548, 551, 772
Bme18I GGWCC 2 cut(s) 194, 295
BmeT110I CYCGRG 1 cut(s) 355
BmgBI CACGTC 2 cut(s) 235, 307
BmgT120I GGNCC 3 cut(s) 194, 295, 363
BmiI GGNNCC 7 cut(s) 68, 108, 195, 196, 271, 365, 694
BmsI GCATC 1 cut(s) 757
BplI GAGNNNNNCTC 2 cut(s) 161, 193
Bpu10I CCTNAGC 2 cut(s) 287, 492
BpuEI CTTGAG 1 cut(s) 329
BsaAI YACGTR 2 cut(s) 133, 745
BsaHI GRCGYC 3 cut(s) 67, 252, 693
BsaJI CCNNGG 3 cut(s) 70, 354, 367
Bsc4I CCNNNNNNNGG 8 cut(s) 190, 191, 220, 238, 266, 289, 360, 563
BseDI CCNNGG 3 cut(s) 70, 354, 367
BseGI GGATG 2 cut(s) 172, 228
BseLI CCNNNNNNNGG 8 cut(s) 190, 191, 220, 238, 266, 289, 360, 563
BseMII CTCAG 1 cut(s) 506
BseRI GAGGAG 1 cut(s) 800
BseXI GCAGC 3 cut(s) 558, 561, 782
Bsh1236I CGCG 1 cut(s) 440
BshFI GGCC 1 cut(s) 364
BshNI GGYRCC 2 cut(s) 66, 692
BsiHKAI GWGCWC 1 cut(s) 322
BsiHKCI CYCGRG 1 cut(s) 355
BsiSI CCGG 2 cut(s) 9, 702
BsiWI CGTACG 1 cut(s) 621
BslFI GGGAC 4 cut(s) 180, 324, 539, 688
BslI CCNNNNNNNGG 8 cut(s) 190, 191, 220, 238, 266, 289, 360, 563
BsmAI GTCTC 2 cut(s) 164, 471
BsmBI CGTCTC 1 cut(s) 164
BsmFI GGGAC 4 cut(s) 180, 324, 539, 688
BsnI GGCC 1 cut(s) 364
BsoBI CYCGRG 1 cut(s) 355
Bsp1286I GDGCHC 1 cut(s) 322
Bsp143I GATC 7 cut(s) 106, 180, 264, 269, 456, 592, 790
BspACI CCGC 8 cut(s) 39, 242, 273, 284, 401, 438, 615, 773
BspANI GGCC 1 cut(s) 364
BspCNI CTCAG 1 cut(s) 505
BspFNI CGCG 1 cut(s) 440
BspLI GGNNCC 7 cut(s) 68, 108, 195, 196, 271, 365, 694
BspPI GGATC 7 cut(s) 101, 114, 175, 264, 277, 464, 798
BspQI GCTCTTC 1 cut(s) 650
BspT107I GGYRCC 2 cut(s) 66, 692
BssECI CCNNGG 3 cut(s) 70, 354, 367
BssMI GATC 7 cut(s) 106, 180, 264, 269, 456, 592, 790
BssNI GRCGYC 3 cut(s) 67, 252, 693
Bst4CI ACNGT 4 cut(s) 324, 394, 465, 572
Bst6I CTCTTC 1 cut(s) 650
BstACI GRCGYC 3 cut(s) 67, 252, 693
BstBAI YACGTR 2 cut(s) 133, 745
BstC8I GCNNGC 1 cut(s) 401
BstDEI CTNAG 2 cut(s) 287, 492
BstDSI CCRYGG 2 cut(s) 70, 367
BstENI CCTNNNNNAGG 1 cut(s) 358
BstF5I GGATG 2 cut(s) 172, 228
BstFNI CGCG 1 cut(s) 440
BstH2I RGCGCY 3 cut(s) 70, 352, 696
BstHHI GCGC 5 cut(s) 69, 351, 442, 488, 695
BstKTI GATC 7 cut(s) 109, 183, 267, 272, 459, 595, 793
BstMAI GTCTC 2 cut(s) 164, 471
BstMBI GATC 7 cut(s) 106, 180, 264, 269, 456, 592, 790
BstMWI GCNNNNNNNGC 3 cut(s) 521, 692, 767
BstUI CGCG 1 cut(s) 440
BstV1I GCAGC 3 cut(s) 558, 561, 782
BstX2I RGATCY 3 cut(s) 106, 269, 790
BstXI CCANNNNNNTGG 1 cut(s) 133
BstYI RGATCY 3 cut(s) 106, 269, 790
BsuRI GGCC 1 cut(s) 364
BtgI CCRYGG 2 cut(s) 70, 367
BtrI CACGTC 2 cut(s) 235, 307
BtsCI GGATG 2 cut(s) 172, 228
Cac8I GCNNGC 1 cut(s) 401
CfoI GCGC 5 cut(s) 69, 351, 442, 488, 695
Cfr13I GGNCC 3 cut(s) 194, 295, 363
CseI GACGC 2 cut(s) 181, 541
Csp6I GTAC 3 cut(s) 568, 622, 628
CviAII CATG 1 cut(s) 162
CviQI GTAC 3 cut(s) 568, 622, 628
DdeI CTNAG 2 cut(s) 287, 492
DinI GGCGCC 2 cut(s) 68, 694
DpnI GATC 7 cut(s) 108, 182, 266, 271, 458, 594, 792
DpnII GATC 7 cut(s) 106, 180, 264, 269, 456, 592, 790
DrdI GACNNNNNNGTC 1 cut(s) 251
DseDI GACNNNNNNGTC 1 cut(s) 251
Eam1104I CTCTTC 1 cut(s) 650
EarI CTCTTC 1 cut(s) 650
EciI GGCGGA 2 cut(s) 28, 257
Eco47I GGWCC 2 cut(s) 194, 295
Eco57I CTGAAG 2 cut(s) 101, 750
Eco72I CACGTG 1 cut(s) 133
Eco88I CYCGRG 1 cut(s) 355
EcoNI CCTNNNNNAGG 1 cut(s) 358
EcoO109I RGGNCCY 3 cut(s) 194, 295, 363
EgeI GGCGCC 2 cut(s) 68, 694
EheI GGCGCC 2 cut(s) 68, 694
Esp3I CGTCTC 1 cut(s) 164
FaeI CATG 1 cut(s) 165
FaiI YATR 6 cut(s) 163, 453, 501, 513, 606, 801
FaqI GGGAC 4 cut(s) 180, 324, 539, 688
FatI CATG 1 cut(s) 161
FauI CCCGC 4 cut(s) 291, 408, 445, 608
Fnu4HI GCNGC 3 cut(s) 547, 550, 771
FokI GGATG 2 cut(s) 179, 215
Fsp4HI GCNGC 3 cut(s) 547, 550, 771
GlaI GCGC 5 cut(s) 68, 350, 441, 487, 694
GluI GCNGC 3 cut(s) 547, 550, 771
HaeII RGCGCY 3 cut(s) 70, 352, 696
HaeIII GGCC 1 cut(s) 364
HapII CCGG 2 cut(s) 9, 702
HgaI GACGC 2 cut(s) 181, 541
HhaI GCGC 5 cut(s) 69, 351, 442, 488, 695
Hin1I GRCGYC 3 cut(s) 67, 252, 693
Hin1II CATG 1 cut(s) 165
Hin6I GCGC 5 cut(s) 67, 349, 440, 486, 693
HinP1I GCGC 5 cut(s) 67, 349, 440, 486, 693
HinfI GANTC 4 cut(s) 152, 206, 312, 598
HpaII CCGG 2 cut(s) 9, 702
Hpy166II GTNNAC 4 cut(s) 136, 223, 331, 716
Hpy188I TCNGA 4 cut(s) 269, 317, 597, 640
Hpy188III TCNNGA 3 cut(s) 178, 474, 673
Hpy8I GTNNAC 4 cut(s) 136, 223, 331, 716
Hpy99I CGWCG 4 cut(s) 152, 203, 469, 640
HpyAV CCTTC 2 cut(s) 643, 725
HpyCH4III ACNGT 4 cut(s) 324, 394, 465, 572
HpyCH4IV ACGT 6 cut(s) 57, 132, 234, 252, 306, 744
HpyCH4V TGCA 1 cut(s) 770
HpyF10VI GCNNNNNNNGC 3 cut(s) 521, 692, 767
HpyF3I CTNAG 2 cut(s) 287, 492
HpySE526I ACGT 6 cut(s) 57, 132, 234, 252, 306, 744
Hsp92I GRCGYC 3 cut(s) 67, 252, 693
Hsp92II CATG 1 cut(s) 165
HspAI GCGC 5 cut(s) 67, 349, 440, 486, 693
KasI GGCGCC 2 cut(s) 66, 692
KflI GGGWCCC 1 cut(s) 194
Kzo9I GATC 7 cut(s) 106, 180, 264, 269, 456, 592, 790
LguI GCTCTTC 1 cut(s) 650
LmnI GCTCC 2 cut(s) 128, 317
LpnPI CCDG 6 cut(s) 22, 432, 534, 549, 663, 715
Lsp1109I GCAGC 3 cut(s) 558, 561, 782
LweI GCATC 1 cut(s) 757
MaeII ACGT 6 cut(s) 57, 132, 234, 252, 306, 744
MaeIII GTNAC 5 cut(s) 25, 186, 388, 407, 572
MalI GATC 7 cut(s) 108, 182, 266, 271, 458, 594, 792
MboI GATC 7 cut(s) 106, 180, 264, 269, 456, 592, 790
MboII GAAGA 2 cut(s) 27, 667
MflI RGATCY 3 cut(s) 106, 269, 790
MhlI GDGCHC 1 cut(s) 322
MluCI AATT 3 cut(s) 30, 61, 502
Mly113I GGCGCC 2 cut(s) 67, 693
MlyI GAGTC 4 cut(s) 146, 200, 306, 607
MmeI TCCRAC 2 cut(s) 357, 618
MslI CAYNNNNRTG 1 cut(s) 131
MspA1I CMGCKG 1 cut(s) 773
MspI CCGG 2 cut(s) 9, 702
MvnI CGCG 1 cut(s) 440
MwoI GCNNNNNNNGC 3 cut(s) 521, 692, 767
NarI GGCGCC 2 cut(s) 67, 693
NdeII GATC 7 cut(s) 106, 180, 264, 269, 456, 592, 790
NlaIII CATG 1 cut(s) 165
NlaIV GGNNCC 7 cut(s) 68, 108, 195, 196, 271, 365, 694
NmuCI GTSAC 2 cut(s) 25, 388
OliI CACNNNNGTG 1 cut(s) 131
PciSI GCTCTTC 1 cut(s) 650
PcsI WCGNNNNNNNCGW 3 cut(s) 144, 403, 644
Pfl23II CGTACG 1 cut(s) 621
PkrI GCNGC 3 cut(s) 548, 551, 772
PleI GAGTC 4 cut(s) 146, 200, 306, 606
PluTI GGCGCC 2 cut(s) 70, 696
PmaCI CACGTG 1 cut(s) 133
PmlI CACGTG 1 cut(s) 133
PpsI GAGTC 4 cut(s) 146, 200, 306, 606
Ppu21I YACGTR 2 cut(s) 133, 745
PpuMI RGGWCCY 2 cut(s) 194, 295
Psp5II RGGWCCY 2 cut(s) 194, 295
PspCI CACGTG 1 cut(s) 133
PspLI CGTACG 1 cut(s) 621
PspN4I GGNNCC 7 cut(s) 68, 108, 195, 196, 271, 365, 694
PspPI GGNCC 3 cut(s) 194, 295, 363
PspPPI RGGWCCY 2 cut(s) 194, 295
PsuI RGATCY 3 cut(s) 106, 269, 790
RsaI GTAC 3 cut(s) 569, 623, 629
RsaNI GTAC 3 cut(s) 568, 622, 628
RseI CAYNNNNRTG 1 cut(s) 131
SapI GCTCTTC 1 cut(s) 650
SatI GCNGC 3 cut(s) 547, 550, 771
Sau3AI GATC 7 cut(s) 106, 180, 264, 269, 456, 592, 790
Sau96I GGNCC 3 cut(s) 194, 295, 363
SchI GAGTC 4 cut(s) 146, 200, 306, 607
SduI GDGCHC 1 cut(s) 322
SfaNI GCATC 1 cut(s) 757
SfoI GGCGCC 2 cut(s) 68, 694
SinI GGWCC 2 cut(s) 194, 295
SmiMI CAYNNNNRTG 1 cut(s) 131
SmlI CTYRAG 1 cut(s) 344
SmoI CTYRAG 1 cut(s) 344
Sse9I AATT 3 cut(s) 30, 61, 502
SsiI CCGC 8 cut(s) 39, 242, 273, 284, 401, 438, 615, 773
SspDI GGCGCC 2 cut(s) 66, 692
SspI AATATT 1 cut(s) 536
TaaI ACNGT 4 cut(s) 324, 394, 465, 572
TaiI ACGT 6 cut(s) 60, 135, 237, 255, 309, 747
TaqI TCGA 5 cut(s) 179, 201, 209, 300, 473
TasI AATT 3 cut(s) 30, 61, 502
TseFI GTSAC 2 cut(s) 25, 388
TseI GCWGC 3 cut(s) 546, 549, 770
Tsp45I GTSAC 2 cut(s) 25, 388
TspGWI ACGGA 2 cut(s) 87, 316
VpaK11BI GGWCC 2 cut(s) 194, 295
XagI CCTNNNNNAGG 1 cut(s) 358
ZraI GACGTC 1 cut(s) 253
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.