MD01G1205900.v1.1

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0004046
Physical Location & Seq
Reverse (-)
30155027 .. 30155905
879 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1205900.v1.1.491

Sequence Viewer

Length: 879 bp
ATGGCGTCCGGTCACGAAGTCCAACTGAAGCTAACCTCCGCCCGCGACCTCAAGAACGTCAACTGGCGAAACGGCGCCTTGAAGCCCTACGCCGTCGTTTGGGTGGACCCCAAGAACAAATGCTCCTCCCACGTCGATGAGGAGGGCGACGCGTCACCCTACTGGGACGAGACCCTCACCATCCCCTTACCGGGCCCCGTTGACGGCGACACCACTCTCAACATCGACATCGTGCACGCCGGATCCGACCCGGATACCAAGCCCTTGATTGGGTCGGCCCGGCTCAAGCTCCGGGATGTCATCGATGACGTGGGCTTCGACGAGCCCGCCAGCCGCACACTTCAGCTCAAGCGACCATCCGGTAGGCCCCAGGGGAAAGTTGACGTTAAAGTTACGATCAGGGAGCCGCGCTATCGTGCGCCGGAACCGTACAACGCTCCTCCTTATGGTGTCCCGCCGCCAGCTGGCTCTCGGGAATATCCCGCTCCGCCAACGGCTTATGGGGCACCTTACGGCGTACCTGCACCACCGCCGCCGCAAAACCCTTACTACGCGGCGCCTCAGCCTTCTGGGTATCCGTATAACCAATACAGTGCTCCAGCGCCGCCGTACGGTCAGCCCGCTCCCTACGGTCAGATGGGACAAGGGAGTTACGGACAGCCGGGGCAGGGCTACGGGCAGGGGGCGTACGGGCAGGAGGAGAAGAAGAGCAAGTTTGGGGGGATGGGGACGGGACTGGCGGTAGGCGCGGTGGCTGGGGCGGTGGGTGGGCTGGCATTGGCGGAGGGGTTTGATTACGTGGAGGACAAAATTGCGGATGACGTGGCTGAGAAAGTGGAGGATGATGTGTACGACGGCGACGACGGCGATGACTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

293

Amino Acids

31.06

Weight (kDa)

4.63

Isoelectric Point (pI)

37.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 6 - 101 2.1e-10 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 529
AccB1I GGYRCC 3 cut(s) 74, 505, 556
AccBSI CCGCTC 2 cut(s) 485, 623
AccII CGCG 5 cut(s) 45, 152, 409, 554, 749
AclWI GGATC 2 cut(s) 237, 250
AcuI CTGAAG 2 cut(s) 47, 326
AcyI GRCGYC 3 cut(s) 5, 75, 557
AfaI GTAC 5 cut(s) 431, 519, 611, 689, 851
AflIII ACRYGT 1 cut(s) 150
AgsI TTSAA 1 cut(s) 82
AjiI CACGTC 3 cut(s) 133, 310, 823
AjnI CCWGG 1 cut(s) 369
AloI GAACNNNNNNTCC 2 cut(s) 107, 139
AluBI AGCT 4 cut(s) 31, 289, 346, 464
AluI AGCT 4 cut(s) 31, 289, 346, 464
Alw21I GWGCWC 2 cut(s) 237, 598
Alw26I GTCTC 1 cut(s) 164
Alw44I GTGCAC 1 cut(s) 233
AlwI GGATC 2 cut(s) 237, 250
Ama87I CYCGRG 1 cut(s) 471
AoxI GGCC 3 cut(s) 193, 276, 365
ApaI GGGCCC 1 cut(s) 197
ApaLI GTGCAC 1 cut(s) 233
ArsI GACNNNNNNTTYG 2 cut(s) 299, 331
AspLEI GCGC 6 cut(s) 77, 411, 421, 559, 604, 749
AspS9I GGNCC 5 cut(s) 106, 193, 194, 277, 366
AsuC2I CCSGG 5 cut(s) 192, 251, 280, 293, 663
AsuHPI GGTGA 2 cut(s) 147, 169
AvaI CYCGRG 1 cut(s) 471
AvaII GGWCC 1 cut(s) 106
BaeGI GKGCMC 3 cut(s) 197, 237, 508
BamHI GGATCC 1 cut(s) 242
BanI GGYRCC 3 cut(s) 74, 505, 556
BanII GRGCYC 2 cut(s) 197, 327
Bbv12I GWGCWC 2 cut(s) 237, 598
BbvCI CCTCAGC 1 cut(s) 561
BccI CCATC 4 cut(s) 188, 364, 631, 718
BceAI ACGGC 7 cut(s) 77, 88, 220, 510, 529, 592, 871
BciT130I CCWGG 1 cut(s) 371
BciVI GTATCC 2 cut(s) 247, 585
BcnI CCSGG 5 cut(s) 192, 251, 280, 293, 663
BcoDI GTCTC 1 cut(s) 164
BfaI CTAG 1 cut(s) 877
BfoI RGCGCY 3 cut(s) 78, 560, 605
BfuAI ACCTGC 1 cut(s) 529
BfuI GTATCC 2 cut(s) 247, 585
BisI GCNGC 7 cut(s) 334, 407, 458, 533, 536, 555, 605
BlsI GCNGC 7 cut(s) 335, 408, 459, 534, 537, 556, 606
Bme1390I CCNGG 6 cut(s) 192, 251, 280, 293, 371, 663
Bme18I GGWCC 1 cut(s) 106
BmeT110I CYCGRG 1 cut(s) 471
BmgBI CACGTC 3 cut(s) 133, 310, 823
BmgT120I GGNCC 5 cut(s) 106, 193, 194, 277, 366
BmrFI CCNGG 6 cut(s) 192, 251, 280, 293, 371, 663
BmrI ACTGGG 1 cut(s) 172
BmuI ACTGGG 1 cut(s) 172
BpmI CTGGAG 1 cut(s) 582
Bpu10I CCTNAGC 1 cut(s) 561
BpuEI CTTGAG 3 cut(s) 35, 269, 332
BpuMI CCSGG 5 cut(s) 192, 251, 280, 293, 663
Bsa29I ATCGAT 1 cut(s) 303
BsaAI YACGTR 1 cut(s) 799
BsaHI GRCGYC 3 cut(s) 5, 75, 557
BsaI GGTCTC 1 cut(s) 164
BsaJI CCNNGG 3 cut(s) 369, 370, 662
BsaWI WCCGGW 2 cut(s) 8, 359
BsaXI ACNNNNNCTCC 2 cut(s) 107, 137
Bse1I ACTGG 3 cut(s) 68, 167, 741
BseBI CCWGG 1 cut(s) 371
BseCI ATCGAT 1 cut(s) 303
BseDI CCNNGG 3 cut(s) 369, 370, 662
BseGI GGATG 6 cut(s) 180, 301, 356, 729, 823, 847
BseMII CTCAG 2 cut(s) 575, 819
BseNI ACTGG 3 cut(s) 68, 167, 741
BseRI GAGGAG 4 cut(s) 115, 155, 429, 713
BseSI GKGCMC 3 cut(s) 197, 237, 508
BseYI CCCAGC 1 cut(s) 755
BsgI GTGCAG 1 cut(s) 507
Bsh1236I CGCG 5 cut(s) 45, 152, 409, 554, 749
BshFI GGCC 3 cut(s) 195, 278, 367
BshNI GGYRCC 3 cut(s) 74, 505, 556
BshVI ATCGAT 1 cut(s) 303
BsiHKAI GWGCWC 2 cut(s) 237, 598
BsiHKCI CYCGRG 1 cut(s) 471
BsiSI CCGG 9 cut(s) 9, 191, 240, 251, 280, 292, 360, 422, 662
BsiWI CGTACG 2 cut(s) 609, 687
BslFI GGGAC 5 cut(s) 179, 437, 654, 742, 747
BsmAI GTCTC 1 cut(s) 164
BsmFI GGGAC 5 cut(s) 179, 437, 654, 742, 747
BsnI GGCC 3 cut(s) 195, 278, 367
Bso31I GGTCTC 1 cut(s) 164
BsoBI CYCGRG 1 cut(s) 471
Bsp120I GGGCCC 1 cut(s) 193
Bsp1286I GDGCHC 5 cut(s) 197, 237, 327, 508, 598
Bsp143I GATC 2 cut(s) 242, 396
BspANI GGCC 3 cut(s) 195, 278, 367
BspCNI CTCAG 2 cut(s) 574, 820
BspDI ATCGAT 1 cut(s) 303
BspFNI CGCG 5 cut(s) 45, 152, 409, 554, 749
BspMI ACCTGC 1 cut(s) 529
BspPI GGATC 2 cut(s) 237, 250
BspQI GCTCTTC 1 cut(s) 701
BspT107I GGYRCC 3 cut(s) 74, 505, 556
BspTNI GGTCTC 1 cut(s) 164
BsrBI CCGCTC 2 cut(s) 485, 623
BsrI ACTGG 3 cut(s) 68, 167, 741
BssECI CCNNGG 3 cut(s) 369, 370, 662
BssMI GATC 2 cut(s) 242, 396
BssNI GRCGYC 3 cut(s) 5, 75, 557
Bst2UI CCWGG 1 cut(s) 371
Bst4CI ACNGT 4 cut(s) 429, 593, 614, 632
Bst6I CTCTTC 1 cut(s) 701
BstACI GRCGYC 3 cut(s) 5, 75, 557
BstBAI YACGTR 1 cut(s) 799
BstC8I GCNNGC 8 cut(s) 43, 237, 327, 331, 462, 466, 621, 774
BstDEI CTNAG 2 cut(s) 561, 828
BstF5I GGATG 6 cut(s) 180, 301, 356, 729, 823, 847
BstFNI CGCG 5 cut(s) 45, 152, 409, 554, 749
BstH2I RGCGCY 3 cut(s) 78, 560, 605
BstHHI GCGC 6 cut(s) 77, 411, 421, 559, 604, 749
BstKTI GATC 2 cut(s) 245, 399
BstMAI GTCTC 1 cut(s) 164
BstMBI GATC 2 cut(s) 242, 396
BstMWI GCNNNNNNNGC 3 cut(s) 503, 746, 864
BstNI CCWGG 1 cut(s) 371
BstSCI CCNGG 6 cut(s) 190, 249, 278, 291, 369, 661
BstSLI GKGCMC 3 cut(s) 197, 237, 508
BstUI CGCG 5 cut(s) 45, 152, 409, 554, 749
BstX2I RGATCY 1 cut(s) 242
BstYI RGATCY 1 cut(s) 242
Bsu15I ATCGAT 1 cut(s) 303
BsuI GTATCC 2 cut(s) 247, 585
BsuRI GGCC 3 cut(s) 195, 278, 367
BsuTUI ATCGAT 1 cut(s) 303
BtrI CACGTC 3 cut(s) 133, 310, 823
BtsCI GGATG 6 cut(s) 180, 301, 356, 729, 823, 847
BtsIMutI CAGTG 1 cut(s) 598
BveI ACCTGC 1 cut(s) 529
Cac8I GCNNGC 8 cut(s) 43, 237, 327, 331, 462, 466, 621, 774
CfoI GCGC 6 cut(s) 77, 411, 421, 559, 604, 749
Cfr13I GGNCC 5 cut(s) 106, 193, 194, 277, 366
ClaI ATCGAT 1 cut(s) 303
CseI GACGC 2 cut(s) 141, 158
Csp6I GTAC 5 cut(s) 430, 518, 610, 688, 850
CviQI GTAC 5 cut(s) 430, 518, 610, 688, 850
DdeI CTNAG 2 cut(s) 561, 828
DinI GGCGCC 2 cut(s) 76, 558
DpnI GATC 2 cut(s) 244, 398
DpnII GATC 2 cut(s) 242, 396
Eam1104I CTCTTC 1 cut(s) 701
EarI CTCTTC 1 cut(s) 701
EciI GGCGGA 3 cut(s) 28, 477, 797
Eco24I GRGCYC 2 cut(s) 197, 327
Eco31I GGTCTC 1 cut(s) 164
Eco47I GGWCC 1 cut(s) 106
Eco57I CTGAAG 2 cut(s) 47, 326
Eco88I CYCGRG 1 cut(s) 471
EcoO109I RGGNCCY 2 cut(s) 194, 366
EcoRII CCWGG 1 cut(s) 369
EcoT38I GRGCYC 2 cut(s) 197, 327
EgeI GGCGCC 2 cut(s) 76, 558
EheI GGCGCC 2 cut(s) 76, 558
FaiI YATR 3 cut(s) 447, 501, 582
FaqI GGGAC 5 cut(s) 179, 437, 654, 742, 747
FauI CCCGC 5 cut(s) 50, 334, 462, 490, 628
Fnu4HI GCNGC 7 cut(s) 334, 407, 458, 533, 536, 555, 605
FokI GGATG 6 cut(s) 167, 308, 343, 736, 830, 854
FriOI GRGCYC 2 cut(s) 197, 327
Fsp4HI GCNGC 7 cut(s) 334, 407, 458, 533, 536, 555, 605
FspBI CTAG 1 cut(s) 877
GlaI GCGC 6 cut(s) 76, 410, 420, 558, 603, 748
GluI GCNGC 7 cut(s) 334, 407, 458, 533, 536, 555, 605
GsaI CCCAGC 1 cut(s) 759
GsuI CTGGAG 1 cut(s) 582
HaeII RGCGCY 3 cut(s) 78, 560, 605
HaeIII GGCC 3 cut(s) 195, 278, 367
HapII CCGG 9 cut(s) 9, 191, 240, 251, 280, 292, 360, 422, 662
HgaI GACGC 2 cut(s) 141, 158
HhaI GCGC 6 cut(s) 77, 411, 421, 559, 604, 749
Hin1I GRCGYC 3 cut(s) 5, 75, 557
Hin6I GCGC 6 cut(s) 75, 409, 419, 557, 602, 747
HinP1I GCGC 6 cut(s) 75, 409, 419, 557, 602, 747
HincII GTYRAC 3 cut(s) 61, 202, 382
HindII GTYRAC 3 cut(s) 61, 202, 382
HpaII CCGG 9 cut(s) 9, 191, 240, 251, 280, 292, 360, 422, 662
HphI GGTGA 2 cut(s) 147, 169
Hpy166II GTNNAC 6 cut(s) 61, 106, 202, 235, 382, 850
Hpy188I TCNGA 2 cut(s) 247, 636
Hpy188III TCNNGA 3 cut(s) 14, 52, 473
Hpy8I GTNNAC 6 cut(s) 61, 106, 202, 235, 382, 850
Hpy99I CGWCG 7 cut(s) 98, 137, 152, 323, 857, 863, 866
HpyAV CCTTC 1 cut(s) 576
HpyCH4III ACNGT 4 cut(s) 429, 593, 614, 632
HpyCH4IV ACGT 6 cut(s) 57, 132, 309, 384, 798, 822
HpyCH4V TGCA 2 cut(s) 235, 524
HpyF10VI GCNNNNNNNGC 3 cut(s) 503, 746, 864
HpyF3I CTNAG 2 cut(s) 561, 828
HpySE526I ACGT 6 cut(s) 57, 132, 309, 384, 798, 822
Hsp92I GRCGYC 3 cut(s) 5, 75, 557
HspAI GCGC 6 cut(s) 75, 409, 419, 557, 602, 747
KasI GGCGCC 2 cut(s) 74, 556
Kzo9I GATC 2 cut(s) 242, 396
LguI GCTCTTC 1 cut(s) 701
LmnI GCTCC 7 cut(s) 128, 294, 403, 442, 490, 601, 628
MaeI CTAG 1 cut(s) 877
MaeII ACGT 6 cut(s) 57, 132, 309, 384, 798, 822
MaeIII GTNAC 4 cut(s) 11, 153, 391, 650
MalI GATC 2 cut(s) 244, 398
MbiI CCGCTC 2 cut(s) 485, 623
MboI GATC 2 cut(s) 242, 396
MboII GAAGA 2 cut(s) 715, 718
MflI RGATCY 1 cut(s) 242
MhlI GDGCHC 5 cut(s) 197, 237, 327, 508, 598
MluCI AATT 1 cut(s) 810
MluI ACGCGT 1 cut(s) 150
Mly113I GGCGCC 2 cut(s) 75, 557
MmeI TCCRAC 2 cut(s) 46, 270
MseI TTAA 1 cut(s) 387
MslI CAYNNNNRTG 1 cut(s) 135
MspA1I CMGCKG 1 cut(s) 464
MspI CCGG 9 cut(s) 9, 191, 240, 251, 280, 292, 360, 422, 662
MspR9I CCNGG 6 cut(s) 192, 251, 280, 293, 371, 663
MvaI CCWGG 1 cut(s) 371
MvnI CGCG 5 cut(s) 45, 152, 409, 554, 749
MwoI GCNNNNNNNGC 3 cut(s) 503, 746, 864
NarI GGCGCC 2 cut(s) 75, 557
NciI CCSGG 5 cut(s) 192, 251, 280, 293, 663
NdeII GATC 2 cut(s) 242, 396
NmuCI GTSAC 2 cut(s) 11, 153
PasI CCCWGGG 1 cut(s) 370
PciSI GCTCTTC 1 cut(s) 701
PcsI WCGNNNNNNNCGW 3 cut(s) 243, 315, 858
Pfl23II CGTACG 2 cut(s) 609, 687
PfoI TCCNGGA 1 cut(s) 291
PkrI GCNGC 7 cut(s) 335, 408, 459, 534, 537, 556, 606
PluTI GGCGCC 2 cut(s) 78, 560
Ppu21I YACGTR 1 cut(s) 799
Psp6I CCWGG 1 cut(s) 369
PspFI CCCAGC 1 cut(s) 755
PspGI CCWGG 1 cut(s) 369
PspLI CGTACG 2 cut(s) 609, 687
PspOMI GGGCCC 1 cut(s) 193
PspPI GGNCC 5 cut(s) 106, 193, 194, 277, 366
PsuI RGATCY 1 cut(s) 242
PvuII CAGCTG 1 cut(s) 464
RsaI GTAC 5 cut(s) 431, 519, 611, 689, 851
RsaNI GTAC 5 cut(s) 430, 518, 610, 688, 850
RseI CAYNNNNRTG 1 cut(s) 135
SapI GCTCTTC 1 cut(s) 701
SaqAI TTAA 1 cut(s) 387
SatI GCNGC 7 cut(s) 334, 407, 458, 533, 536, 555, 605
Sau3AI GATC 2 cut(s) 242, 396
Sau96I GGNCC 5 cut(s) 106, 193, 194, 277, 366
ScrFI CCNGG 6 cut(s) 192, 251, 280, 293, 371, 663
SduI GDGCHC 5 cut(s) 197, 237, 327, 508, 598
SfoI GGCGCC 2 cut(s) 76, 558
SinI GGWCC 1 cut(s) 106
SmiMI CAYNNNNRTG 1 cut(s) 135
SmlI CTYRAG 3 cut(s) 50, 284, 347
SmoI CTYRAG 3 cut(s) 50, 284, 347
Sse9I AATT 1 cut(s) 810
SspDI GGCGCC 2 cut(s) 74, 556
SspMI CTAG 1 cut(s) 877
StyD4I CCNGG 6 cut(s) 190, 249, 278, 291, 369, 661
TaaI ACNGT 4 cut(s) 429, 593, 614, 632
TaiI ACGT 6 cut(s) 60, 135, 312, 387, 801, 825
TaqI TCGA 4 cut(s) 135, 225, 303, 318
TasI AATT 1 cut(s) 810
TauI GCSGC 7 cut(s) 336, 409, 460, 535, 538, 557, 607
Tru1I TTAA 1 cut(s) 387
Tru9I TTAA 1 cut(s) 387
TscAI CASTG 1 cut(s) 598
TseFI GTSAC 2 cut(s) 11, 153
Tsp45I GTSAC 2 cut(s) 11, 153
TspGWI ACGGA 2 cut(s) 567, 669
TspRI CASTG 1 cut(s) 598
VneI GTGCAC 1 cut(s) 233
VpaK11BI GGWCC 1 cut(s) 106
XspI CTAG 1 cut(s) 877
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.