Rroxscaffold_180G00433600

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000180
OG0004046
Physical Location & Seq
Reverse (-)
674087 .. 679007
4921 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_180G00433600.1

Sequence Viewer

Length: 942 bp
ATGTGCCGGCAAAGGTCATTTGAACCATCACCAGCATCGATGGATCTTCAACAGCAAGCCTTTCTCGACTCCATCACCTCATTTATATCTTCTAAATCTCTCTTACTCGATCAGAAAATGGCTTCCGGCTACGAAGTAGAGGTCACAATTTCTTCCGCCAAAGACCTCAAAAACGTGAATTGGCGCCACGGAGAGCTGAAGCCCTACGCTGTTGTTTGGGTGGATCCAAACAATAAATGCTCGACCCACGTAGACGAATACGGCGACGACTCGCCCACGTGGGATGAGACGCTCTCGATCCCGTTACGGGGTTCCGTCGAGGACTCGACCCTTTTCGTGGACATCATCCACGTCAAGGCGGACGAGGACGTCAAGCCGTTGATCGGATCCGCAAAACTCCCGCTTAGGGAGGTCCTCGATGACGTGGGGCTTGGGGCACCGTCCGTTCACAAGTTGAAGCTCAAGCGCCCCTCGGGTAGGCCCCACGGGAAGTTGGAAGTCAAAGTCACAGTTCGCCCGCCCCGTTACCAAGCACCTGACCCGTATCACGCCCCCGCGCCCTACGGGTACGGGATCCCACCGCCGCAGTCGAGAGACTACGGCGGACCTCAGCAGTATAATTATCAATATGCCCCTCCTGCCCAACCCCAATATTACCAAAGCAGCAGCGTCCCACAAGCAGGGTACGGTTACAATCAGCCATCTTACGATCAGAGTCAGTATGGGAGTGGCGGGTCGTACGGGTACGGGTCGTCGGAGAAGCCGAAGGAGAAGAGCAAGTTTGGGGTCGGGACCGGATTGGCTGTGGGAGCCGTAGCCGGAGTGTTGGGTGGACTTGCGATTGCTGAAGGAGTGGATTACGTGGAGGACAAGATTGCTGATGATGCAGCGGAGAAAGTGGAGGAGGATCTTGGTTATGATGATTTTGGCGATGATGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

313

Amino Acids

34.13

Weight (kDa)

4.83

Isoelectric Point (pI)

42.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 46 - 139 2e-10 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 368
AatII GACGTC 1 cut(s) 372
AccB1I GGYRCC 2 cut(s) 183, 436
AccI GTMKAC 1 cut(s) 252
AccII CGCG 1 cut(s) 557
AclWI GGATC 9 cut(s) 51, 218, 231, 292, 381, 394, 568, 581, 915
AcuI CTGAAG 2 cut(s) 218, 867
AcvI CACGTG 1 cut(s) 279
AcyI GRCGYC 2 cut(s) 184, 369
AfaI GTAC 4 cut(s) 569, 686, 740, 746
AfiI CCNNNNNNNGG 8 cut(s) 307, 308, 337, 355, 383, 406, 477, 680
AgsI TTSAA 3 cut(s) 23, 50, 457
AjiI CACGTC 2 cut(s) 352, 424
AluBI AGCT 2 cut(s) 196, 460
AluI AGCT 2 cut(s) 196, 460
Alw26I GTCTC 2 cut(s) 281, 588
AlwI GGATC 9 cut(s) 51, 218, 231, 292, 381, 394, 568, 581, 915
Ama87I CYCGRG 1 cut(s) 472
AoxI GGCC 1 cut(s) 479
ApeKI GCWGC 3 cut(s) 663, 666, 887
ArsI GACNNNNNNTTYG 2 cut(s) 126, 158
AspLEI GCGC 3 cut(s) 186, 468, 559
AspS9I GGNCC 4 cut(s) 412, 480, 605, 792
AsuHPI GGTGA 2 cut(s) 21, 67
AvaI CYCGRG 1 cut(s) 472
AvaII GGWCC 3 cut(s) 412, 605, 792
BaeGI GKGCMC 1 cut(s) 439
BamHI GGATCC 3 cut(s) 223, 386, 573
BanI GGYRCC 2 cut(s) 183, 436
BbrPI CACGTG 1 cut(s) 279
BbvCI CCTCAGC 1 cut(s) 609
BbvI GCAGC 3 cut(s) 675, 678, 899
BccI CCATC 4 cut(s) 34, 34, 80, 709
BceAI ACGGC 4 cut(s) 277, 361, 616, 797
BcoDI GTCTC 2 cut(s) 281, 588
BfoI RGCGCY 2 cut(s) 187, 469
BisI GCNGC 4 cut(s) 584, 664, 667, 888
BlsI GCNGC 4 cut(s) 585, 665, 668, 889
Bme18I GGWCC 3 cut(s) 412, 605, 792
BmeT110I CYCGRG 1 cut(s) 472
BmgBI CACGTC 2 cut(s) 352, 424
BmgT120I GGNCC 4 cut(s) 412, 480, 605, 792
BmiI GGNNCC 9 cut(s) 185, 225, 313, 388, 438, 482, 575, 793, 811
BmsI GCATC 2 cut(s) 44, 874
BplI GAGNNNNNCTC 2 cut(s) 278, 310
Bpu10I CCTNAGC 2 cut(s) 404, 609
BpuEI CTTGAG 1 cut(s) 446
Bsa29I ATCGAT 1 cut(s) 38
BsaAI YACGTR 3 cut(s) 250, 279, 862
BsaHI GRCGYC 2 cut(s) 184, 369
BsaJI CCNNGG 3 cut(s) 187, 471, 484
BsaWI WCCGGW 1 cut(s) 794
Bsc4I CCNNNNNNNGG 8 cut(s) 307, 308, 337, 355, 383, 406, 477, 680
Bse118I RCCGGY 1 cut(s) 6
BseCI ATCGAT 1 cut(s) 38
BseDI CCNNGG 3 cut(s) 187, 471, 484
BseGI GGATG 2 cut(s) 289, 345
BseLI CCNNNNNNNGG 8 cut(s) 307, 308, 337, 355, 383, 406, 477, 680
BseMII CTCAG 1 cut(s) 623
BseRI GAGGAG 1 cut(s) 917
BseSI GKGCMC 1 cut(s) 439
BseXI GCAGC 3 cut(s) 675, 678, 899
Bsh1236I CGCG 1 cut(s) 557
BshFI GGCC 1 cut(s) 481
BshNI GGYRCC 2 cut(s) 183, 436
BshVI ATCGAT 1 cut(s) 38
BsiHKCI CYCGRG 1 cut(s) 472
BsiSI CCGG 4 cut(s) 7, 126, 795, 819
BsiWI CGTACG 1 cut(s) 738
BslFI GGGAC 2 cut(s) 656, 805
BslI CCNNNNNNNGG 8 cut(s) 307, 308, 337, 355, 383, 406, 477, 680
BsmAI GTCTC 2 cut(s) 281, 588
BsmBI CGTCTC 1 cut(s) 281
BsmFI GGGAC 2 cut(s) 656, 805
BsnI GGCC 1 cut(s) 481
BsoBI CYCGRG 1 cut(s) 472
Bsp1286I GDGCHC 1 cut(s) 439
Bsp143I GATC 9 cut(s) 43, 109, 223, 297, 381, 386, 573, 709, 907
BspANI GGCC 1 cut(s) 481
BspCNI CTCAG 1 cut(s) 622
BspDI ATCGAT 1 cut(s) 38
BspFNI CGCG 1 cut(s) 557
BspLI GGNNCC 9 cut(s) 185, 225, 313, 388, 438, 482, 575, 793, 811
BspPI GGATC 9 cut(s) 51, 218, 231, 292, 381, 394, 568, 581, 915
BspQI GCTCTTC 1 cut(s) 767
BspT107I GGYRCC 2 cut(s) 183, 436
BsrFI RCCGGY 1 cut(s) 6
BssAI RCCGGY 1 cut(s) 6
BssECI CCNNGG 3 cut(s) 187, 471, 484
BssMI GATC 9 cut(s) 43, 109, 223, 297, 381, 386, 573, 709, 907
BssNI GRCGYC 2 cut(s) 184, 369
Bst4CI ACNGT 3 cut(s) 441, 511, 689
Bst6I CTCTTC 1 cut(s) 767
BstACI GRCGYC 2 cut(s) 184, 369
BstBAI YACGTR 3 cut(s) 250, 279, 862
BstC8I GCNNGC 3 cut(s) 8, 57, 518
BstDEI CTNAG 2 cut(s) 404, 609
BstDSI CCRYGG 2 cut(s) 187, 484
BstENI CCTNNNNNAGG 1 cut(s) 475
BstF5I GGATG 2 cut(s) 289, 345
BstFNI CGCG 1 cut(s) 557
BstH2I RGCGCY 2 cut(s) 187, 469
BstHHI GCGC 3 cut(s) 186, 468, 559
BstKTI GATC 9 cut(s) 46, 112, 226, 300, 384, 389, 576, 712, 910
BstMAI GTCTC 2 cut(s) 281, 588
BstMBI GATC 9 cut(s) 43, 109, 223, 297, 381, 386, 573, 709, 907
BstMWI GCNNNNNNNGC 3 cut(s) 638, 809, 884
BstSLI GKGCMC 1 cut(s) 439
BstUI CGCG 1 cut(s) 557
BstV1I GCAGC 3 cut(s) 675, 678, 899
BstX2I RGATCY 5 cut(s) 43, 223, 386, 573, 907
BstYI RGATCY 5 cut(s) 43, 223, 386, 573, 907
Bsu15I ATCGAT 1 cut(s) 38
BsuRI GGCC 1 cut(s) 481
BsuTUI ATCGAT 1 cut(s) 38
BtgI CCRYGG 2 cut(s) 187, 484
BtrI CACGTC 2 cut(s) 352, 424
BtsCI GGATG 2 cut(s) 289, 345
Cac8I GCNNGC 3 cut(s) 8, 57, 518
CfoI GCGC 3 cut(s) 186, 468, 559
Cfr10I RCCGGY 1 cut(s) 6
Cfr13I GGNCC 4 cut(s) 412, 480, 605, 792
ClaI ATCGAT 1 cut(s) 38
CseI GACGC 2 cut(s) 298, 658
Csp6I GTAC 4 cut(s) 568, 685, 739, 745
CviQI GTAC 4 cut(s) 568, 685, 739, 745
DdeI CTNAG 2 cut(s) 404, 609
DinI GGCGCC 1 cut(s) 185
DpnI GATC 9 cut(s) 45, 111, 225, 299, 383, 388, 575, 711, 909
DpnII GATC 9 cut(s) 43, 109, 223, 297, 381, 386, 573, 709, 907
DrdI GACNNNNNNGTC 1 cut(s) 368
DseDI GACNNNNNNGTC 1 cut(s) 368
Eam1104I CTCTTC 1 cut(s) 767
EarI CTCTTC 1 cut(s) 767
EciI GGCGGA 3 cut(s) 145, 374, 618
Eco47I GGWCC 3 cut(s) 412, 605, 792
Eco57I CTGAAG 2 cut(s) 218, 867
Eco72I CACGTG 1 cut(s) 279
Eco88I CYCGRG 1 cut(s) 472
EcoNI CCTNNNNNAGG 1 cut(s) 475
EcoO109I RGGNCCY 2 cut(s) 412, 480
EgeI GGCGCC 1 cut(s) 185
EheI GGCGCC 1 cut(s) 185
Esp3I CGTCTC 1 cut(s) 281
FaiI YATR 5 cut(s) 86, 618, 630, 723, 918
FaqI GGGAC 2 cut(s) 656, 805
FauI CCCGC 4 cut(s) 408, 525, 562, 725
FblI GTMKAC 1 cut(s) 252
Fnu4HI GCNGC 4 cut(s) 584, 664, 667, 888
FokI GGATG 2 cut(s) 296, 332
Fsp4HI GCNGC 4 cut(s) 584, 664, 667, 888
GlaI GCGC 3 cut(s) 185, 467, 558
GluI GCNGC 4 cut(s) 584, 664, 667, 888
HaeII RGCGCY 2 cut(s) 187, 469
HaeIII GGCC 1 cut(s) 481
HapII CCGG 4 cut(s) 7, 126, 795, 819
HgaI GACGC 2 cut(s) 298, 658
HhaI GCGC 3 cut(s) 186, 468, 559
Hin1I GRCGYC 2 cut(s) 184, 369
Hin6I GCGC 3 cut(s) 184, 466, 557
HinP1I GCGC 3 cut(s) 184, 466, 557
HinfI GANTC 4 cut(s) 68, 269, 323, 715
HpaII CCGG 4 cut(s) 7, 126, 795, 819
HphI GGTGA 2 cut(s) 21, 67
Hpy166II GTNNAC 4 cut(s) 253, 340, 448, 833
Hpy188I TCNGA 4 cut(s) 114, 386, 714, 757
Hpy188III TCNNGA 4 cut(s) 65, 295, 591, 790
Hpy8I GTNNAC 4 cut(s) 253, 340, 448, 833
Hpy99I CGWCG 3 cut(s) 269, 320, 757
HpyAV CCTTC 2 cut(s) 760, 842
HpyCH4III ACNGT 3 cut(s) 441, 511, 689
HpyCH4IV ACGT 7 cut(s) 174, 249, 278, 351, 369, 423, 861
HpyCH4V TGCA 1 cut(s) 887
HpyF10VI GCNNNNNNNGC 3 cut(s) 638, 809, 884
HpyF3I CTNAG 2 cut(s) 404, 609
HpySE526I ACGT 7 cut(s) 174, 249, 278, 351, 369, 423, 861
Hsp92I GRCGYC 2 cut(s) 184, 369
HspAI GCGC 3 cut(s) 184, 466, 557
KasI GGCGCC 1 cut(s) 183
KroI GCCGGC 1 cut(s) 6
KroNI GCCGGC 1 cut(s) 8
Kzo9I GATC 9 cut(s) 43, 109, 223, 297, 381, 386, 573, 709, 907
LguI GCTCTTC 1 cut(s) 767
LmnI GCTCC 1 cut(s) 809
LpnPI CCDG 8 cut(s) 20, 45, 139, 549, 651, 666, 808, 832
Lsp1109I GCAGC 3 cut(s) 675, 678, 899
LweI GCATC 2 cut(s) 44, 874
MaeII ACGT 7 cut(s) 174, 249, 278, 351, 369, 423, 861
MaeIII GTNAC 5 cut(s) 142, 303, 505, 524, 689
MalI GATC 9 cut(s) 45, 111, 225, 299, 383, 388, 575, 711, 909
MboI GATC 9 cut(s) 43, 109, 223, 297, 381, 386, 573, 709, 907
MboII GAAGA 4 cut(s) 38, 81, 144, 784
MflI RGATCY 5 cut(s) 43, 223, 386, 573, 907
MhlI GDGCHC 1 cut(s) 439
MluCI AATT 3 cut(s) 147, 178, 619
Mly113I GGCGCC 1 cut(s) 184
MlyI GAGTC 4 cut(s) 62, 263, 317, 724
MmeI TCCRAC 2 cut(s) 474, 735
MroNI GCCGGC 1 cut(s) 6
MspA1I CMGCKG 1 cut(s) 890
MspI CCGG 4 cut(s) 7, 126, 795, 819
MvnI CGCG 1 cut(s) 557
MwoI GCNNNNNNNGC 3 cut(s) 638, 809, 884
NaeI GCCGGC 1 cut(s) 8
NarI GGCGCC 1 cut(s) 184
NdeII GATC 9 cut(s) 43, 109, 223, 297, 381, 386, 573, 709, 907
NgoMIV GCCGGC 1 cut(s) 6
NlaIV GGNNCC 9 cut(s) 185, 225, 313, 388, 438, 482, 575, 793, 811
NmuCI GTSAC 2 cut(s) 142, 505
PciSI GCTCTTC 1 cut(s) 767
PcsI WCGNNNNNNNCGW 3 cut(s) 261, 520, 761
PdiI GCCGGC 1 cut(s) 8
Pfl23II CGTACG 1 cut(s) 738
PkrI GCNGC 4 cut(s) 585, 665, 668, 889
PleI GAGTC 4 cut(s) 62, 263, 317, 723
PluTI GGCGCC 1 cut(s) 187
PmaCI CACGTG 1 cut(s) 279
PmlI CACGTG 1 cut(s) 279
PpsI GAGTC 4 cut(s) 62, 263, 317, 723
Ppu21I YACGTR 3 cut(s) 250, 279, 862
PpuMI RGGWCCY 1 cut(s) 412
Psp5II RGGWCCY 1 cut(s) 412
PspCI CACGTG 1 cut(s) 279
PspLI CGTACG 1 cut(s) 738
PspN4I GGNNCC 9 cut(s) 185, 225, 313, 388, 438, 482, 575, 793, 811
PspPI GGNCC 4 cut(s) 412, 480, 605, 792
PspPPI RGGWCCY 1 cut(s) 412
PsuI RGATCY 5 cut(s) 43, 223, 386, 573, 907
RsaI GTAC 4 cut(s) 569, 686, 740, 746
RsaNI GTAC 4 cut(s) 568, 685, 739, 745
SapI GCTCTTC 1 cut(s) 767
SatI GCNGC 4 cut(s) 584, 664, 667, 888
Sau3AI GATC 9 cut(s) 43, 109, 223, 297, 381, 386, 573, 709, 907
Sau96I GGNCC 4 cut(s) 412, 480, 605, 792
SchI GAGTC 4 cut(s) 62, 263, 317, 724
SduI GDGCHC 1 cut(s) 439
SfaNI GCATC 2 cut(s) 44, 874
SfoI GGCGCC 1 cut(s) 185
SinI GGWCC 3 cut(s) 412, 605, 792
SmlI CTYRAG 1 cut(s) 461
SmoI CTYRAG 1 cut(s) 461
Sse9I AATT 3 cut(s) 147, 178, 619
SspDI GGCGCC 1 cut(s) 183
SspI AATATT 1 cut(s) 653
TaaI ACNGT 3 cut(s) 441, 511, 689
TaiI ACGT 7 cut(s) 177, 252, 281, 354, 372, 426, 864
TaqI TCGA 9 cut(s) 38, 66, 108, 242, 296, 318, 326, 417, 590
TasI AATT 3 cut(s) 147, 178, 619
TauI GCSGC 1 cut(s) 586
TseFI GTSAC 2 cut(s) 142, 505
TseI GCWGC 3 cut(s) 663, 666, 887
Tsp45I GTSAC 2 cut(s) 142, 505
TspGWI ACGGA 3 cut(s) 204, 304, 433
VpaK11BI GGWCC 3 cut(s) 412, 605, 792
XagI CCTNNNNNAGG 1 cut(s) 475
XmiI GTMKAC 1 cut(s) 252
ZraI GACGTC 1 cut(s) 370
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.