AT5G25800

Small RNA degrading nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0003406
Physical Location & Seq
Reverse (-)
8979571 .. 8982891
3321 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G25800.1

Sequence Viewer

Length: 1704 bp
ATGTCTTCTTCGAAGCGGAAGCGCGGCGCCGAAACCGCCGCGGAAGATTGTGACGACGGTACTGATAAGAGCAATACCGGAAACAGTTTCTTCGATATATATGGACCCGAAGCGAAAGCAGAACTCGATTTCAAAAGCCCTGAAACAACATTGAACTTACAAGATGTTCAAGGACTTGTGACATGGGTTCTTGCAGAAGGTTTTATGCCTTCATGGGTATTTATTAAGAACAAGCCTCTCATTCCAAAGGTTGTACTGCTCTACCTTCCTGGATTGGATGCAGCTTTATACTTATCACATTCTAAAACTCTGTCTAGTTTGAAATCATGTTGTGGCAATCCTATGGCGCTTTTAGCTTTGAGCTGTGTAGTTGATGAGATGAGGACAATCGATACAATCCTTACTTGCAAGGGTAGAAAAAAGAAAACTGTTACTAGTTCAGTGGAACCACCTCCCCTTGTATCTAGCCCAGAAGCATGCAATCTGATGGGGAAATCGTTCGTTGAGCTCACGAAAGATATACCATTCCCTGTTAGTTACTACACTCTAAGTCAAAAGGAAATGGAACAAAATGGTTACACTTTTGAAAAACTCGAGTTGACACCTACACTTCCTGCACCATCAGGATCATGTCCCCCCGAAATCGTGGCTCTTGATTGCGAGATGTGTATTACAAAGGAGGGTTTGGAGTTGACGAGAGTGACGCTGGTGGATATCCAAGGACAGGTTCTATTAGATAAATTAGTCATGCCAACAAATCCTATTACTGATTACAACACAAGATATAGTGGAATAACAGCTGTGATGATGGAGGGAGTTACAACAACACTTAAAGATATTCAGGAAGAGTTCTTAAAGCTGGTTTTCAAAGAGACCATATTAGTCGGACACTCCTTGGAAAATGACTTGTTGTCTTTGAAGATCTCTCATAATCTGGTGATTGACACTGCAGTACTATACAAGCATCCACATGGAAGATCTTATAAAACTAAACTTCGAATTCTAGCAAAAAAGTTTCTTGCTAGAGAGATACAGGAGTCTGAATCCGGACATGACAGCGCAGAGGATGCAAAAGCAGCCATGGATTTGGCACTATTGAAGATAAAACATGGACCTGATTTTGGTTCACCACCGGAAGTGATAAGAAAAAAGCTCCTCGCCGTTTTAAATGAGAGCGGGAAAACGACTTCTATAATTGACAATATCAACATCGTGAAGCGATATGCTTCTGAGTCATCAAATGCAATTCCAGTTTCGTCTGACGACGAAGCACTTTCAAAGGCCGTGAAGGAGGTAAAGAACAAGCGGTCTCAATTCGTTTGGACACAGTTCTCAGAATTGAATGCACATTTCCAGAGCAGAGCGGATGATCCACAGAAACTAAACTCCAGACTAGCCGAGATGATCTCATTGCTTACATGTAACAAAAAGTCCGGCCTTAAGAAAAGCAATGTCTCACCTGAAACAAAGGAAATCTTAAAGAAAATGAATGAAAGAGTCCAATCCCTTTATGCTGCATTACCTACAAATGCGATGTTTATAGTTTGCACCGGACACGGAGACACATCCATTGTACACAGAGTGCGGAAAATGCTTAAAGATGAGGATGAAATCGGGTTTGACCGTGAGAAACTTGTCAAGGTTCTTGAAGAGCTTCAAGCACAAGCCGAAGTAGCTCTCTGTTTTGTAGGTATCAAGCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
RNase_T

Protein Analysis

567

Amino Acids

62.8

Weight (kDa)

6.42

Isoelectric Point (pI)

38.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_T PF00929 216 - 363 3.1e-09 Exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 984
AccB1I GGYRCC 1 cut(s) 26
AccBSI CCGCTC 2 cut(s) 1176, 1364
AccII CGCG 2 cut(s) 24, 41
AccIII TCCGGA 1 cut(s) 1046
AciI CCGC 9 cut(s) 16, 24, 36, 39, 41, 1176, 1306, 1364, 1585
AclWI GGATC 2 cut(s) 634, 1364
AcsI RAATTY 1 cut(s) 999
AcyI GRCGYC 1 cut(s) 27
AdeI CACNNNGTG 1 cut(s) 1582
AfaI GTAC 4 cut(s) 61, 255, 954, 1575
AfiI CCNNNNNNNGG 2 cut(s) 724, 1121
AflII CTTAAG 1 cut(s) 1439
AflIII ACRYGT 1 cut(s) 1418
AhdI GACNNNNNGTC 1 cut(s) 910
AhlI ACTAGT 1 cut(s) 434
AjnI CCWGG 1 cut(s) 268
AjuI GAANNNNNNNTTGG 2 cut(s) 711, 743
AluBI AGCT 9 cut(s) 284, 356, 363, 508, 800, 859, 1153, 1654, 1676
AluI AGCT 9 cut(s) 284, 356, 363, 508, 800, 859, 1153, 1654, 1676
Alw21I GWGCWC 1 cut(s) 510
Alw26I GTCTC 4 cut(s) 866, 1314, 1459, 1554
AlwI GGATC 2 cut(s) 634, 1364
Ama87I CYCGRG 1 cut(s) 593
Aor13HI TCCGGA 1 cut(s) 1046
AoxI GGCC 2 cut(s) 1281, 1435
ApeKI GCWGC 3 cut(s) 281, 1076, 1514
ApoI RAATTY 1 cut(s) 999
Asp700I GAANNNNTTC 2 cut(s) 497, 1653
AspLEI GCGC 4 cut(s) 24, 29, 349, 1061
AspS9I GGNCC 2 cut(s) 104, 1112
AsuHPI GGTGA 3 cut(s) 949, 1119, 1449
AsuII TTCGAA 2 cut(s) 11, 997
AvaI CYCGRG 1 cut(s) 593
AvaII GGWCC 2 cut(s) 104, 1112
BaeI ACNNNNGTAYC 2 cut(s) 1022, 1055
BanI GGYRCC 1 cut(s) 26
BanII GRGCYC 1 cut(s) 510
Bbv12I GWGCWC 1 cut(s) 510
BbvI GCAGC 3 cut(s) 293, 1088, 1501
BccI CCATC 3 cut(s) 481, 628, 802
BceAI ACGGC 2 cut(s) 1145, 1268
BciT130I CCWGG 1 cut(s) 270
BcoDI GTCTC 4 cut(s) 866, 1314, 1459, 1554
BcuI ACTAGT 1 cut(s) 434
BfaI CTAG 6 cut(s) 315, 435, 465, 1004, 1023, 1394
BfmI CTRYAG 1 cut(s) 948
BfoI RGCGCY 2 cut(s) 30, 350
BfrI CTTAAG 1 cut(s) 1439
BglII AGATCT 2 cut(s) 921, 977
BisI GCNGC 5 cut(s) 25, 39, 282, 1077, 1515
BlsI GCNGC 5 cut(s) 26, 40, 283, 1078, 1516
BmcAI AGTACT 1 cut(s) 954
Bme1390I CCNGG 1 cut(s) 270
Bme18I GGWCC 2 cut(s) 104, 1112
BmeRI GACNNNNNGTC 1 cut(s) 910
BmeT110I CYCGRG 1 cut(s) 593
BmgT120I GGNCC 2 cut(s) 104, 1112
BmiI GGNNCC 3 cut(s) 28, 106, 447
BmrFI CCNGG 1 cut(s) 270
BmsI GCATC 3 cut(s) 268, 973, 1057
BplI GAGNNNNNCTC 2 cut(s) 1391, 1423
BpmI CTGGAG 1 cut(s) 1372
Bpu14I TTCGAA 2 cut(s) 11, 997
Bsa29I ATCGAT 1 cut(s) 390
BsaHI GRCGYC 1 cut(s) 27
BsaI GGTCTC 2 cut(s) 866, 1314
BsaJI CCNNGG 4 cut(s) 39, 718, 894, 1080
BsaWI WCCGGW 4 cut(s) 77, 1046, 1132, 1550
Bsc4I CCNNNNNNNGG 2 cut(s) 724, 1121
Bse1I ACTGG 1 cut(s) 1250
Bse3DI GCAATG 2 cut(s) 1409, 1456
BseAI TCCGGA 1 cut(s) 1046
BseBI CCWGG 1 cut(s) 270
BseCI ATCGAT 1 cut(s) 390
BseDI CCNNGG 4 cut(s) 39, 718, 894, 1080
BseGI GGATG 6 cut(s) 283, 964, 1072, 1372, 1565, 1612
BseLI CCNNNNNNNGG 2 cut(s) 724, 1121
BseMI GCAATG 2 cut(s) 1409, 1456
BseMII CTCAG 2 cut(s) 1221, 1347
BseNI ACTGG 1 cut(s) 1250
BseRI GAGGAG 1 cut(s) 1145
BseXI GCAGC 3 cut(s) 293, 1088, 1501
BsgI GTGCAG 1 cut(s) 600
Bsh1236I CGCG 2 cut(s) 24, 41
BshFI GGCC 2 cut(s) 1283, 1437
BshNI GGYRCC 1 cut(s) 26
BshVI ATCGAT 1 cut(s) 390
BsiHKAI GWGCWC 1 cut(s) 510
BsiHKCI CYCGRG 1 cut(s) 593
BsiSI CCGG 5 cut(s) 78, 1047, 1133, 1434, 1551
BslFI GGGAC 1 cut(s) 618
BslI CCNNNNNNNGG 2 cut(s) 724, 1121
BsmAI GTCTC 4 cut(s) 866, 1314, 1459, 1554
BsmFI GGGAC 1 cut(s) 618
BsmI GAATGC 1 cut(s) 1348
BsnI GGCC 2 cut(s) 1283, 1437
Bso31I GGTCTC 2 cut(s) 866, 1314
BsoBI CYCGRG 1 cut(s) 593
Bsp119I TTCGAA 2 cut(s) 11, 997
Bsp1286I GDGCHC 1 cut(s) 510
Bsp13I TCCGGA 1 cut(s) 1046
Bsp1407I TGTACA 1 cut(s) 1573
Bsp143I GATC 5 cut(s) 626, 921, 977, 1369, 1404
Bsp19I CCATGG 1 cut(s) 1080
BspACI CCGC 9 cut(s) 16, 24, 36, 39, 41, 1176, 1306, 1364, 1585
BspANI GGCC 2 cut(s) 1283, 1437
BspCNI CTCAG 2 cut(s) 1222, 1346
BspDI ATCGAT 1 cut(s) 390
BspEI TCCGGA 1 cut(s) 1046
BspFNI CGCG 2 cut(s) 24, 41
BspLI GGNNCC 3 cut(s) 28, 106, 447
BspMAI CTGCAG 1 cut(s) 952
BspPI GGATC 2 cut(s) 634, 1364
BspQI GCTCTTC 1 cut(s) 1644
BspT104I TTCGAA 2 cut(s) 11, 997
BspT107I GGYRCC 1 cut(s) 26
BspTI CTTAAG 1 cut(s) 1439
BspTNI GGTCTC 2 cut(s) 866, 1314
BsrBI CCGCTC 2 cut(s) 1176, 1364
BsrDI GCAATG 2 cut(s) 1409, 1456
BsrGI TGTACA 1 cut(s) 1573
BsrI ACTGG 1 cut(s) 1250
BssECI CCNNGG 4 cut(s) 39, 718, 894, 1080
BssMI GATC 5 cut(s) 626, 921, 977, 1369, 1404
BssNI GRCGYC 1 cut(s) 27
BssT1I CCWWGG 3 cut(s) 718, 894, 1080
Bst2UI CCWGG 1 cut(s) 270
Bst4CI ACNGT 5 cut(s) 59, 86, 430, 1329, 1625
Bst6I CTCTTC 2 cut(s) 840, 1644
BstACI GRCGYC 1 cut(s) 27
BstAFI CTTAAG 1 cut(s) 1439
BstAPI GCANNNNNTGC 1 cut(s) 1067
BstAUI TGTACA 1 cut(s) 1573
BstBI TTCGAA 2 cut(s) 11, 997
BstC8I GCNNGC 1 cut(s) 478
BstDEI CTNAG 3 cut(s) 548, 1230, 1333
BstDSI CCRYGG 2 cut(s) 39, 1080
BstF5I GGATG 6 cut(s) 283, 964, 1072, 1372, 1565, 1612
BstFNI CGCG 2 cut(s) 24, 41
BstH2I RGCGCY 2 cut(s) 30, 350
BstHHI GCGC 4 cut(s) 24, 29, 349, 1061
BstKTI GATC 5 cut(s) 629, 924, 980, 1372, 1407
BstMAI GTCTC 4 cut(s) 866, 1314, 1459, 1554
BstMBI GATC 5 cut(s) 626, 921, 977, 1369, 1404
BstMWI GCNNNNNNNGC 6 cut(s) 35, 353, 1067, 1076, 1591, 1673
BstNI CCWGG 1 cut(s) 270
BstNSI RCATGY 2 cut(s) 480, 1422
BstSCI CCNGG 1 cut(s) 268
BstSFI CTRYAG 1 cut(s) 948
BstUI CGCG 2 cut(s) 24, 41
BstV1I GCAGC 3 cut(s) 293, 1088, 1501
BstX2I RGATCY 2 cut(s) 921, 977
BstXI CCANNNNNNTGG 1 cut(s) 1087
BstYI RGATCY 2 cut(s) 921, 977
Bsu15I ATCGAT 1 cut(s) 390
BsuRI GGCC 2 cut(s) 1283, 1437
BsuTUI ATCGAT 1 cut(s) 390
BtgI CCRYGG 2 cut(s) 39, 1080
BtgZI GCGATG 1 cut(s) 1547
BtsCI GGATG 6 cut(s) 283, 964, 1072, 1372, 1565, 1612
BtsI GCAGTG 1 cut(s) 945
BtsIMutI CAGTG 2 cut(s) 447, 945
Cac8I GCNNGC 1 cut(s) 478
CfoI GCGC 4 cut(s) 24, 29, 349, 1061
Cfr13I GGNCC 2 cut(s) 104, 1112
Cfr42I CCGCGG 1 cut(s) 42
ClaI ATCGAT 1 cut(s) 390
CseI GACGC 1 cut(s) 712
Csp6I GTAC 4 cut(s) 60, 254, 953, 1574
CviQI GTAC 4 cut(s) 60, 254, 953, 1574
DdeI CTNAG 3 cut(s) 548, 1230, 1333
DinI GGCGCC 1 cut(s) 28
DpnI GATC 5 cut(s) 628, 923, 979, 1371, 1406
DpnII GATC 5 cut(s) 626, 921, 977, 1369, 1404
DraI TTTAAA 1 cut(s) 1167
DraIII CACNNNGTG 1 cut(s) 1582
DriI GACNNNNNGTC 1 cut(s) 910
Eam1104I CTCTTC 2 cut(s) 840, 1644
Eam1105I GACNNNNNGTC 1 cut(s) 910
EarI CTCTTC 2 cut(s) 840, 1644
Ecl136II GAGCTC 1 cut(s) 508
Eco130I CCWWGG 3 cut(s) 718, 894, 1080
Eco24I GRGCYC 1 cut(s) 510
Eco31I GGTCTC 2 cut(s) 866, 1314
Eco32I GATATC 1 cut(s) 715
Eco47I GGWCC 2 cut(s) 104, 1112
Eco53kI GAGCTC 1 cut(s) 508
Eco88I CYCGRG 1 cut(s) 593
EcoICRI GAGCTC 1 cut(s) 508
EcoRI GAATTC 1 cut(s) 999
EcoRII CCWGG 1 cut(s) 268
EcoRV GATATC 1 cut(s) 715
EcoT14I CCWWGG 3 cut(s) 718, 894, 1080
EcoT38I GRGCYC 1 cut(s) 510
EgeI GGCGCC 1 cut(s) 28
EheI GGCGCC 1 cut(s) 28
ErhI CCWWGG 3 cut(s) 718, 894, 1080
FalI AAGNNNNNCTT 2 cut(s) 1460, 1492
FaqI GGGAC 1 cut(s) 618
FauI CCCGC 1 cut(s) 1169
Fnu4HI GCNGC 5 cut(s) 25, 39, 282, 1077, 1515
FokI GGATG 6 cut(s) 290, 951, 1079, 1379, 1552, 1619
FriOI GRGCYC 1 cut(s) 510
Fsp4HI GCNGC 5 cut(s) 25, 39, 282, 1077, 1515
FspBI CTAG 6 cut(s) 315, 435, 465, 1004, 1023, 1394
GlaI GCGC 4 cut(s) 23, 28, 348, 1060
GluI GCNGC 5 cut(s) 25, 39, 282, 1077, 1515
GsuI CTGGAG 1 cut(s) 1372
HaeII RGCGCY 2 cut(s) 30, 350
HaeIII GGCC 2 cut(s) 1283, 1437
HapII CCGG 5 cut(s) 78, 1047, 1133, 1434, 1551
HgaI GACGC 1 cut(s) 712
HhaI GCGC 4 cut(s) 24, 29, 349, 1061
Hin1I GRCGYC 1 cut(s) 27
Hin6I GCGC 4 cut(s) 22, 27, 347, 1059
HinP1I GCGC 4 cut(s) 22, 27, 347, 1059
HincII GTYRAC 2 cut(s) 600, 693
HindII GTYRAC 2 cut(s) 600, 693
HinfI GANTC 4 cut(s) 1037, 1043, 1232, 1497
HpaII CCGG 5 cut(s) 78, 1047, 1133, 1434, 1551
HphI GGTGA 3 cut(s) 949, 1119, 1449
Hpy166II GTNNAC 4 cut(s) 600, 693, 1127, 1576
Hpy188I TCNGA 6 cut(s) 486, 887, 1042, 1231, 1261, 1336
Hpy188III TCNNGA 9 cut(s) 511, 624, 653, 842, 1047, 1213, 1354, 1389, 1646
Hpy8I GTNNAC 4 cut(s) 600, 693, 1127, 1576
Hpy99I CGWCG 2 cut(s) 59, 1268
HpyAV CCTTC 4 cut(s) 191, 219, 275, 1282
HpyCH4III ACNGT 5 cut(s) 59, 86, 430, 1329, 1625
HpyF10VI GCNNNNNNNGC 6 cut(s) 35, 353, 1067, 1076, 1591, 1673
HpyF3I CTNAG 3 cut(s) 548, 1230, 1333
Hsp92I GRCGYC 1 cut(s) 27
HspAI GCGC 4 cut(s) 22, 27, 347, 1059
KasI GGCGCC 1 cut(s) 26
Kpn2I TCCGGA 1 cut(s) 1046
KspI CCGCGG 1 cut(s) 42
Kzo9I GATC 5 cut(s) 626, 921, 977, 1369, 1404
LguI GCTCTTC 1 cut(s) 1644
LmnI GCTCC 1 cut(s) 1158
Lsp1109I GCAGC 3 cut(s) 293, 1088, 1501
LweI GCATC 3 cut(s) 268, 973, 1057
MaeI CTAG 6 cut(s) 315, 435, 465, 1004, 1023, 1394
MaeIII GTNAC 8 cut(s) 50, 178, 430, 536, 575, 700, 817, 1421
MalI GATC 5 cut(s) 628, 923, 979, 1371, 1406
MbiI CCGCTC 2 cut(s) 1176, 1364
MboI GATC 5 cut(s) 626, 921, 977, 1369, 1404
MboII GAAGA 7 cut(s) 56, 82, 857, 931, 987, 1111, 1661
MflI RGATCY 2 cut(s) 921, 977
MhlI GDGCHC 1 cut(s) 510
MluCI AATT 6 cut(s) 740, 999, 1194, 1245, 1313, 1337
Mly113I GGCGCC 1 cut(s) 27
MlyI GAGTC 3 cut(s) 1046, 1241, 1506
MmeI TCCRAC 1 cut(s) 865
MnlI CCTC 9 cut(s) 246, 375, 462, 673, 805, 1057, 1166, 1285, 1597
MroI TCCGGA 1 cut(s) 1046
MroXI GAANNNNTTC 2 cut(s) 497, 1653
MseI TTAA 7 cut(s) 225, 831, 854, 1166, 1440, 1478, 1596
MslI CAYNNNNRTG 1 cut(s) 969
MspA1I CMGCKG 2 cut(s) 41, 800
MspCI CTTAAG 1 cut(s) 1439
MspI CCGG 5 cut(s) 78, 1047, 1133, 1434, 1551
MspR9I CCNGG 1 cut(s) 270
MteI GCGCNGCGC 1 cut(s) 25
Mva1269I GAATGC 1 cut(s) 1348
MvaI CCWGG 1 cut(s) 270
MvnI CGCG 2 cut(s) 24, 41
MwoI GCNNNNNNNGC 6 cut(s) 35, 353, 1067, 1076, 1591, 1673
NarI GGCGCC 1 cut(s) 27
NcoI CCATGG 1 cut(s) 1080
NdeII GATC 5 cut(s) 626, 921, 977, 1369, 1404
NlaIV GGNNCC 3 cut(s) 28, 106, 447
NmeAIII GCCGAG 1 cut(s) 1423
NmuCI GTSAC 3 cut(s) 50, 178, 700
NspI RCATGY 2 cut(s) 480, 1422
NspV TTCGAA 2 cut(s) 11, 997
PaeI GCATGC 1 cut(s) 480
PaeR7I CTCGAG 1 cut(s) 593
PciI ACATGT 1 cut(s) 1418
PciSI GCTCTTC 1 cut(s) 1644
PctI GAATGC 1 cut(s) 1348
PdmI GAANNNNTTC 2 cut(s) 497, 1653
PfeI GAWTC 1 cut(s) 1043
PfoI TCCNGGA 1 cut(s) 268
PkrI GCNGC 5 cut(s) 26, 40, 283, 1078, 1516
PleI GAGTC 3 cut(s) 1045, 1240, 1505
PluTI GGCGCC 1 cut(s) 30
PpsI GAGTC 3 cut(s) 1045, 1240, 1505
PscI ACATGT 1 cut(s) 1418
PsiI TTATAA 1 cut(s) 984
Psp124BI GAGCTC 1 cut(s) 510
Psp6I CCWGG 1 cut(s) 268
PspGI CCWGG 1 cut(s) 268
PspN4I GGNNCC 3 cut(s) 28, 106, 447
PspPI GGNCC 2 cut(s) 104, 1112
PspXI VCTCGAGB 1 cut(s) 593
PstI CTGCAG 1 cut(s) 952
PsuI RGATCY 2 cut(s) 921, 977
PvuII CAGCTG 1 cut(s) 800
RsaI GTAC 4 cut(s) 61, 255, 954, 1575
RsaNI GTAC 4 cut(s) 60, 254, 953, 1574
RseI CAYNNNNRTG 1 cut(s) 969
SacI GAGCTC 1 cut(s) 510
SacII CCGCGG 1 cut(s) 42
SapI GCTCTTC 1 cut(s) 1644
SaqAI TTAA 7 cut(s) 225, 831, 854, 1166, 1440, 1478, 1596
SatI GCNGC 5 cut(s) 25, 39, 282, 1077, 1515
Sau3AI GATC 5 cut(s) 626, 921, 977, 1369, 1404
Sau96I GGNCC 2 cut(s) 104, 1112
ScaI AGTACT 1 cut(s) 954
SchI GAGTC 3 cut(s) 1046, 1241, 1506
ScrFI CCNGG 1 cut(s) 270
SduI GDGCHC 1 cut(s) 510
SfaNI GCATC 3 cut(s) 268, 973, 1057
SfcI CTRYAG 1 cut(s) 948
SfoI GGCGCC 1 cut(s) 28
Sfr274I CTCGAG 1 cut(s) 593
Sfr303I CCGCGG 1 cut(s) 42
SfuI TTCGAA 2 cut(s) 11, 997
SgrBI CCGCGG 1 cut(s) 42
SinI GGWCC 2 cut(s) 104, 1112
SlaI CTCGAG 1 cut(s) 593
SmiMI CAYNNNNRTG 1 cut(s) 969
SmlI CTYRAG 2 cut(s) 593, 1439
SmoI CTYRAG 2 cut(s) 593, 1439
SpeI ACTAGT 1 cut(s) 434
SphI GCATGC 1 cut(s) 480
Sse9I AATT 6 cut(s) 740, 999, 1194, 1245, 1313, 1337
SsiI CCGC 9 cut(s) 16, 24, 36, 39, 41, 1176, 1306, 1364, 1585
SspDI GGCGCC 1 cut(s) 26
SspMI CTAG 6 cut(s) 315, 435, 465, 1004, 1023, 1394
SstI GAGCTC 1 cut(s) 510
StyD4I CCNGG 1 cut(s) 268
StyI CCWWGG 3 cut(s) 718, 894, 1080
TaaI ACNGT 5 cut(s) 59, 86, 430, 1329, 1625
TaqI TCGA 6 cut(s) 11, 93, 126, 390, 594, 997
TasI AATT 6 cut(s) 740, 999, 1194, 1245, 1313, 1337
TatI WGTACW 3 cut(s) 253, 952, 1573
TauI GCSGC 2 cut(s) 27, 41
TfiI GAWTC 1 cut(s) 1043
Tru1I TTAA 7 cut(s) 225, 831, 854, 1166, 1440, 1478, 1596
Tru9I TTAA 7 cut(s) 225, 831, 854, 1166, 1440, 1478, 1596
TscAI CASTG 2 cut(s) 447, 952
TseFI GTSAC 3 cut(s) 50, 178, 700
TseI GCWGC 3 cut(s) 281, 1076, 1514
Tsp45I GTSAC 3 cut(s) 50, 178, 700
TspDTI ATGAA 4 cut(s) 201, 1502, 1506, 1623
TspGWI ACGGA 1 cut(s) 1572
TspRI CASTG 2 cut(s) 447, 952
Vha464I CTTAAG 1 cut(s) 1439
VpaK11BI GGWCC 2 cut(s) 104, 1112
XapI RAATTY 1 cut(s) 999
XceI RCATGY 2 cut(s) 480, 1422
XhoI CTCGAG 1 cut(s) 593
XmnI GAANNNNTTC 2 cut(s) 497, 1653
XspI CTAG 6 cut(s) 315, 435, 465, 1004, 1023, 1394
ZrmI AGTACT 1 cut(s) 954
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.