FvH4_2g15020

Small RNA degrading nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0003406
Physical Location & Seq
Forward (+)
13184350 .. 13191289
6940 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g15020.t2

Sequence Viewer

Length: 1713 bp
ATGCCTTCGTCGCACAGTAAACGCGAAGAAGAAGAAGATATAGCTCCCGAGGAAGACGACTCAAACAGAGACGAAGCCAATGCCAAAACCTCCAATTTCTACGACGTTTACGGCCCTCAAGCTAAAGCAGGTGTGGAGTTCAAGACTCCAGAAGCTAGCTCTACTCTGAATTTCGAAGAAGTTCAACAACTAGTGACGTGGGTGCTTGCTGATGGATTCATGCCGTCTTGGGTTTTTGTTAAGAACAAGCCCCTCATCCCCAAAGTGGTTATGCTTTATGTTCCAGGCCTGGATGCAGCTCTATATTTATCGCAGCATCGACTATTTAAGGGTCTCGAAAAATGCTGTGGCAAACCTAGGCCAGTTTTAGCACTCAGATATATGTCGCATGAAAATCAGACAATTGATGCGCTCCTCACAAGCAAACTAAAAAGAAAGAGGGAAGAAAGTGATCCAGCTAGAACAAAATCCAACCCGGAAAATCAACATGATGATCCATCCCCTGGGGAGCTTCTGAAAGATCTACCTTTCCCAATTACATTTTACACTCTTACAACAAAACAATTGGAAGACAATAATTATGTCCTCAATCAACCAGGTTTTCTTTCTACTCTTCCTGCTCCTTCTGGATCATCTCCTAATGAAATGTTAGCACTTGATTGTGAGATGTGTATCACAGAAGAGGGGTTTGAGCTAACAAGAGCCACGCTGGTGACTATAAAAGGAGAGGTTATACTCGATAAATTGGTTAAACCTACAAATGCCATCAAAGATTATAACACCAGGTATAGTGGAATCACACCTGAGATGTTAGACGGGGTGACTACAAGCCTCAAAGATATTCAGGAGGACTTCCTAAAGCAGGTTTACAAAGAGACTGTCCTAGTTGGACATTCGTTGGAGAATGACTTATTAGCATTAAAAATCAGTCACAATCTCGTGATTGATACAGCAGTATTGTACCAGCACCCTGGTCCGCGATCTTACAAAGCTGCTCTTAGAGTTCTTGCCAAGAGATATCTTAAAAAAGAGATACAGCAGTCAGGGAATGGTCATGACAGTACTGAAGATGCAAGAGCTGCTATGGAACTGGCGCTTTTAAAGATTAGACATGGACCTGACTTTGGTACAGGACCATCAGTTTTCCGGAAAAAGCTACTAAAAGTTTTGAGTGATAATGGAAAGACCACCTCTATCATTGATGATGTTGCAATTGTAAAGCGCTATGCCTCTGAATCATCCCATGCATTTCCTGTGGCCTCTGATGAGGAAGCTCTATCTAAAGCTGTAAAGGAGGCAAGGAATGAGAAAGTGCACTTTGTCTGGACTCAATTCTCAGAATTACATCGTTTTTTAAAGAATCAAGCAGAGGATGCTGAGAAATTAAATGCAAGACTTGCTCAGATGATAAGCATGCTTACATGCCATAAGAAGTCTGGAGATAAAAAAGGTATCAGATGGAGTTTGACTTCTGAACTAAAGGAAATTCTTATTTGCCTGGACACTAGAATACGTTCTCTTTATGCTGCACTGCCCACTAGTGCCATGCTTATCATTTGTACCGGTCATGGAGATATAGCAACTGTAGACAGATTAAACAAAATGCTTTCGGAACCTGTGGAAACCACGATGTGTCGGGATAGCATTGTAAAGGTTTTAGCGGAGCTCAAGGCCCAAGCTGAGGTAGCTTTATGTTTTGTGGGTGTGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
RNase_T

Protein Analysis

571

Amino Acids

63.68

Weight (kDa)

6.21

Isoelectric Point (pI)

37.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_T PF00929 218 - 364 1.4e-10 Exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 777
AarI CACCTGC 1 cut(s) 119
Acc36I ACCTGC 2 cut(s) 119, 853
AccB7I CCANNNNNTGG 1 cut(s) 503
AccI GTMKAC 1 cut(s) 1587
AccII CGCG 2 cut(s) 24, 979
AccIII TCCGGA 1 cut(s) 1146
AciI CCGC 2 cut(s) 977, 1661
AclWI GGATC 3 cut(s) 446, 488, 637
AcsI RAATTY 2 cut(s) 169, 1485
AcuI CTGAAG 1 cut(s) 1086
AdeI CACNNNGTG 1 cut(s) 1632
AfaI GTAC 4 cut(s) 962, 1063, 1129, 1561
AfeI AGCGCT 1 cut(s) 1223
AfiI CCNNNNNNNGG 5 cut(s) 265, 503, 862, 1124, 1681
AgeI ACCGGT 1 cut(s) 1562
AgsI TTSAA 2 cut(s) 142, 185
AhlI ACTAGT 2 cut(s) 190, 1538
AjiI CACGTC 1 cut(s) 198
AjnI CCWGG 7 cut(s) 283, 288, 502, 595, 782, 970, 1497
AleI CACNNNNGTG 1 cut(s) 710
Alw21I GWGCWC 2 cut(s) 1317, 1668
Alw26I GTCTC 3 cut(s) 63, 338, 869
Alw44I GTGCAC 1 cut(s) 1313
AlwI GGATC 3 cut(s) 446, 488, 637
Ama87I CYCGRG 1 cut(s) 47
Aor13HI TCCGGA 1 cut(s) 1146
Aor51HI AGCGCT 1 cut(s) 1223
AoxI GGCC 5 cut(s) 112, 286, 359, 1257, 1671
ApaLI GTGCAC 1 cut(s) 1313
ApeKI GCWGC 5 cut(s) 296, 313, 992, 1079, 1526
ApoI RAATTY 2 cut(s) 169, 1485
ArsI GACNNNNNNTTYG 2 cut(s) 864, 896
AsiGI ACCGGT 1 cut(s) 1562
Asp700I GAANNNNTTC 2 cut(s) 180, 1513
AspA2I CCTAGG 1 cut(s) 356
AspLEI GCGC 3 cut(s) 412, 1096, 1224
AspS9I GGNCC 5 cut(s) 113, 974, 1115, 1133, 1672
AsuC2I CCSGG 1 cut(s) 476
AsuHPI GGTGA 2 cut(s) 724, 832
AsuII TTCGAA 1 cut(s) 174
AsuNHI GCTAGC 1 cut(s) 155
AvaI CYCGRG 1 cut(s) 47
AvaII GGWCC 3 cut(s) 974, 1115, 1133
AvrII CCTAGG 1 cut(s) 356
BaeGI GKGCMC 1 cut(s) 1317
BaeI ACNNNNGTAYC 4 cut(s) 939, 972, 1025, 1058
BanII GRGCYC 1 cut(s) 1668
BauI CACGAG 1 cut(s) 938
BbsI GAAGAC 2 cut(s) 60, 576
Bbv12I GWGCWC 2 cut(s) 1317, 1668
BbvCI CCTCAGC 1 cut(s) 1680
BbvI GCAGC 5 cut(s) 308, 325, 979, 1066, 1513
BccI CCATC 5 cut(s) 206, 505, 773, 1144, 1452
BceAI ACGGC 2 cut(s) 127, 208
BcgI CGANNNNNNTGC 2 cut(s) 62, 96
BciT130I CCWGG 7 cut(s) 285, 290, 504, 597, 784, 972, 1499
BcnI CCSGG 1 cut(s) 476
BcoDI GTCTC 3 cut(s) 63, 338, 869
BcuI ACTAGT 2 cut(s) 190, 1538
BfaI CTAG 7 cut(s) 156, 191, 357, 459, 884, 1506, 1539
BfmI CTRYAG 1 cut(s) 1584
BfoI RGCGCY 2 cut(s) 1097, 1225
BfuAI ACCTGC 2 cut(s) 119, 853
BglII AGATCT 1 cut(s) 520
BisI GCNGC 5 cut(s) 297, 314, 993, 1080, 1527
BlnI CCTAGG 1 cut(s) 356
BlsI GCNGC 5 cut(s) 298, 315, 994, 1081, 1528
BmcAI AGTACT 1 cut(s) 1063
Bme1390I CCNGG 8 cut(s) 285, 290, 476, 504, 597, 784, 972, 1499
Bme18I GGWCC 3 cut(s) 974, 1115, 1133
BmeT110I CYCGRG 1 cut(s) 47
BmgBI CACGTC 1 cut(s) 198
BmgT120I GGNCC 5 cut(s) 113, 974, 1115, 1133, 1672
BmiI GGNNCC 1 cut(s) 1614
BmrFI CCNGG 8 cut(s) 285, 290, 476, 504, 597, 784, 972, 1499
BmsI GCATC 5 cut(s) 283, 325, 397, 1060, 1363
BmtI GCTAGC 1 cut(s) 159
BpiI GAAGAC 2 cut(s) 60, 576
BpmI CTGGAG 2 cut(s) 132, 1458
Bpu10I CCTNAGC 1 cut(s) 1680
Bpu14I TTCGAA 1 cut(s) 174
BpuEI CTTGAG 2 cut(s) 102, 1652
BpuMI CCSGG 1 cut(s) 476
BsaBI GATNNNNATC 1 cut(s) 671
BsaI GGTCTC 1 cut(s) 338
BsaJI CCNNGG 5 cut(s) 48, 356, 502, 503, 970
BsaWI WCCGGW 2 cut(s) 1146, 1562
Bsc4I CCNNNNNNNGG 5 cut(s) 265, 503, 862, 1124, 1681
Bse118I RCCGGY 1 cut(s) 1562
Bse1I ACTGG 2 cut(s) 362, 1095
Bse8I GATNNNNATC 1 cut(s) 671
BseAI TCCGGA 1 cut(s) 1146
BseBI CCWGG 7 cut(s) 285, 290, 504, 597, 784, 972, 1499
BseDI CCNNGG 5 cut(s) 48, 356, 502, 503, 970
BseGI GGATG 5 cut(s) 255, 298, 497, 1238, 1378
BseJI GATNNNNATC 1 cut(s) 671
BseLI CCNNNNNNNGG 5 cut(s) 265, 503, 862, 1124, 1681
BseMII CTCAG 6 cut(s) 388, 795, 1350, 1368, 1415, 1671
BseNI ACTGG 2 cut(s) 362, 1095
BseRI GAGGAG 1 cut(s) 404
BseSI GKGCMC 1 cut(s) 1317
BseXI GCAGC 5 cut(s) 308, 325, 979, 1066, 1513
BsgI GTGCAG 1 cut(s) 1512
Bsh1236I CGCG 2 cut(s) 24, 979
BshFI GGCC 5 cut(s) 114, 288, 361, 1259, 1673
BshTI ACCGGT 1 cut(s) 1562
BsiHKAI GWGCWC 2 cut(s) 1317, 1668
BsiHKCI CYCGRG 1 cut(s) 47
BsiSI CCGG 3 cut(s) 476, 1147, 1563
BslI CCNNNNNNNGG 5 cut(s) 265, 503, 862, 1124, 1681
BsmAI GTCTC 3 cut(s) 63, 338, 869
BsmBI CGTCTC 1 cut(s) 63
BsnI GGCC 5 cut(s) 114, 288, 361, 1259, 1673
Bso31I GGTCTC 1 cut(s) 338
BsoBI CYCGRG 1 cut(s) 47
Bsp119I TTCGAA 1 cut(s) 174
Bsp1286I GDGCHC 2 cut(s) 1317, 1668
Bsp13I TCCGGA 1 cut(s) 1146
Bsp143I GATC 5 cut(s) 451, 493, 520, 629, 980
BspACI CCGC 2 cut(s) 977, 1661
BspANI GGCC 5 cut(s) 114, 288, 361, 1259, 1673
BspCNI CTCAG 6 cut(s) 387, 796, 1349, 1369, 1414, 1672
BspEI TCCGGA 1 cut(s) 1146
BspFNI CGCG 2 cut(s) 24, 979
BspHI TCATGA 1 cut(s) 1054
BspLI GGNNCC 1 cut(s) 1614
BspMI ACCTGC 2 cut(s) 119, 853
BspOI GCTAGC 1 cut(s) 159
BspPI GGATC 3 cut(s) 446, 488, 637
BspT104I TTCGAA 1 cut(s) 174
BspTNI GGTCTC 1 cut(s) 338
BsrFI RCCGGY 1 cut(s) 1562
BsrI ACTGG 2 cut(s) 362, 1095
BssAI RCCGGY 1 cut(s) 1562
BssECI CCNNGG 5 cut(s) 48, 356, 502, 503, 970
BssMI GATC 5 cut(s) 451, 493, 520, 629, 980
BssSI CACGAG 1 cut(s) 938
BssT1I CCWWGG 1 cut(s) 356
Bst2BI CACGAG 1 cut(s) 938
Bst2UI CCWGG 7 cut(s) 285, 290, 504, 597, 784, 972, 1499
Bst4CI ACNGT 4 cut(s) 17, 880, 1061, 1585
Bst6I CTCTTC 2 cut(s) 618, 675
BstAPI GCANNNNNTGC 3 cut(s) 1079, 1373, 1397
BstBI TTCGAA 1 cut(s) 174
BstC8I GCNNGC 3 cut(s) 157, 207, 1415
BstDEI CTNAG 7 cut(s) 374, 804, 998, 1336, 1377, 1401, 1680
BstENI CCTNNNNNAGG 1 cut(s) 860
BstF5I GGATG 5 cut(s) 255, 298, 497, 1238, 1378
BstFNI CGCG 2 cut(s) 24, 979
BstH2I RGCGCY 2 cut(s) 1097, 1225
BstHHI GCGC 3 cut(s) 412, 1096, 1224
BstKTI GATC 5 cut(s) 454, 496, 523, 632, 983
BstMAI GTCTC 3 cut(s) 63, 338, 869
BstMBI GATC 5 cut(s) 451, 493, 520, 629, 980
BstMWI GCNNNNNNNGC 5 cut(s) 10, 1079, 1373, 1397, 1685
BstNI CCWGG 7 cut(s) 285, 290, 504, 597, 784, 972, 1499
BstNSI RCATGY 2 cut(s) 1417, 1425
BstSCI CCNGG 8 cut(s) 283, 288, 474, 502, 595, 782, 970, 1497
BstSFI CTRYAG 1 cut(s) 1584
BstSLI GKGCMC 1 cut(s) 1317
BstUI CGCG 2 cut(s) 24, 979
BstV1I GCAGC 5 cut(s) 308, 325, 979, 1066, 1513
BstV2I GAAGAC 2 cut(s) 60, 576
BstX2I RGATCY 1 cut(s) 520
BstXI CCANNNNNNTGG 1 cut(s) 971
BstYI RGATCY 1 cut(s) 520
BsuRI GGCC 5 cut(s) 114, 288, 361, 1259, 1673
BtrI CACGTC 1 cut(s) 198
BtsCI GGATG 5 cut(s) 255, 298, 497, 1238, 1378
BtsI GCAGTG 1 cut(s) 1529
BtsIMutI CAGTG 1 cut(s) 1529
BveI ACCTGC 2 cut(s) 119, 853
Cac8I GCNNGC 3 cut(s) 157, 207, 1415
CciI TCATGA 1 cut(s) 1054
CfoI GCGC 3 cut(s) 412, 1096, 1224
Cfr10I RCCGGY 1 cut(s) 1562
Cfr13I GGNCC 5 cut(s) 113, 974, 1115, 1133, 1672
CsiI ACCWGGT 2 cut(s) 595, 782
Csp6I GTAC 4 cut(s) 961, 1062, 1128, 1560
CspAI ACCGGT 1 cut(s) 1562
CviQI GTAC 4 cut(s) 961, 1062, 1128, 1560
DdeI CTNAG 7 cut(s) 374, 804, 998, 1336, 1377, 1401, 1680
DpnI GATC 5 cut(s) 453, 495, 522, 631, 982
DpnII GATC 5 cut(s) 451, 493, 520, 629, 980
DraI TTTAAA 2 cut(s) 1101, 1356
DraIII CACNNNGTG 1 cut(s) 1632
Eam1104I CTCTTC 2 cut(s) 618, 675
EarI CTCTTC 2 cut(s) 618, 675
Ecl136II GAGCTC 1 cut(s) 1666
Eco130I CCWWGG 1 cut(s) 356
Eco147I AGGCCT 1 cut(s) 288
Eco24I GRGCYC 1 cut(s) 1668
Eco31I GGTCTC 1 cut(s) 338
Eco32I GATATC 1 cut(s) 1019
Eco47I GGWCC 3 cut(s) 974, 1115, 1133
Eco47III AGCGCT 1 cut(s) 1223
Eco53kI GAGCTC 1 cut(s) 1666
Eco57I CTGAAG 1 cut(s) 1086
Eco88I CYCGRG 1 cut(s) 47
EcoICRI GAGCTC 1 cut(s) 1666
EcoNI CCTNNNNNAGG 1 cut(s) 860
EcoRII CCWGG 7 cut(s) 283, 288, 502, 595, 782, 970, 1497
EcoRV GATATC 1 cut(s) 1019
EcoT14I CCWWGG 1 cut(s) 356
EcoT22I ATGCAT 1 cut(s) 1249
EcoT38I GRGCYC 1 cut(s) 1668
ErhI CCWWGG 1 cut(s) 356
Esp3I CGTCTC 1 cut(s) 63
FalI AAGNNNNNCTT 2 cut(s) 981, 1013
FblI GTMKAC 1 cut(s) 1587
Fnu4HI GCNGC 5 cut(s) 297, 314, 993, 1080, 1527
FokI GGATG 5 cut(s) 242, 305, 484, 1225, 1385
FriOI GRGCYC 1 cut(s) 1668
Fsp4HI GCNGC 5 cut(s) 297, 314, 993, 1080, 1527
FspBI CTAG 7 cut(s) 156, 191, 357, 459, 884, 1506, 1539
GlaI GCGC 3 cut(s) 411, 1095, 1223
GluI GCNGC 5 cut(s) 297, 314, 993, 1080, 1527
GsuI CTGGAG 2 cut(s) 132, 1458
HaeII RGCGCY 2 cut(s) 1097, 1225
HaeIII GGCC 5 cut(s) 114, 288, 361, 1259, 1673
HapII CCGG 3 cut(s) 476, 1147, 1563
HhaI GCGC 3 cut(s) 412, 1096, 1224
Hin6I GCGC 3 cut(s) 410, 1094, 1222
HinP1I GCGC 3 cut(s) 410, 1094, 1222
HinfI GANTC 7 cut(s) 59, 145, 216, 795, 1235, 1327, 1360
HpaII CCGG 3 cut(s) 476, 1147, 1563
HphI GGTGA 2 cut(s) 724, 832
Hpy166II GTNNAC 5 cut(s) 20, 109, 868, 1315, 1588
Hpy8I GTNNAC 5 cut(s) 20, 109, 868, 1315, 1588
Hpy99I CGWCG 2 cut(s) 13, 107
HpyAV CCTTC 2 cut(s) 15, 633
HpyCH4III ACNGT 4 cut(s) 17, 880, 1061, 1585
HpyCH4IV ACGT 3 cut(s) 105, 197, 1513
HpyCH4V TGCA 7 cut(s) 296, 1073, 1211, 1247, 1315, 1391, 1529
HpyF10VI GCNNNNNNNGC 5 cut(s) 10, 1079, 1373, 1397, 1685
HpyF3I CTNAG 7 cut(s) 374, 804, 998, 1336, 1377, 1401, 1680
HpySE526I ACGT 3 cut(s) 105, 197, 1513
HspAI GCGC 3 cut(s) 410, 1094, 1222
Kpn2I TCCGGA 1 cut(s) 1146
Kzo9I GATC 5 cut(s) 451, 493, 520, 629, 980
LmnI GCTCC 5 cut(s) 49, 417, 508, 625, 1663
Lsp1109I GCAGC 5 cut(s) 308, 325, 979, 1066, 1513
LweI GCATC 5 cut(s) 283, 325, 397, 1060, 1363
MabI ACCWGGT 2 cut(s) 595, 782
MaeI CTAG 7 cut(s) 156, 191, 357, 459, 884, 1506, 1539
MaeII ACGT 3 cut(s) 105, 197, 1513
MaeIII GTNAC 4 cut(s) 193, 712, 820, 929
MalI GATC 5 cut(s) 453, 495, 522, 631, 982
MboI GATC 5 cut(s) 451, 493, 520, 629, 980
MfeI CAATTG 3 cut(s) 402, 563, 1212
MflI RGATCY 1 cut(s) 520
MhlI GDGCHC 2 cut(s) 1317, 1668
MlyI GAGTC 3 cut(s) 53, 139, 1321
MmeI TCCRAC 3 cut(s) 495, 868, 879
Mph1103I ATGCAT 1 cut(s) 1249
MroI TCCGGA 1 cut(s) 1146
MroXI GAANNNNTTC 2 cut(s) 180, 1513
MseI TTAA 9 cut(s) 240, 327, 750, 920, 1023, 1100, 1355, 1385, 1595
MslI CAYNNNNRTG 1 cut(s) 710
MspI CCGG 3 cut(s) 476, 1147, 1563
MspR9I CCNGG 8 cut(s) 285, 290, 476, 504, 597, 784, 972, 1499
MunI CAATTG 3 cut(s) 402, 563, 1212
MvaI CCWGG 7 cut(s) 285, 290, 504, 597, 784, 972, 1499
MvnI CGCG 2 cut(s) 24, 979
MwoI GCNNNNNNNGC 5 cut(s) 10, 1079, 1373, 1397, 1685
NciI CCSGG 1 cut(s) 476
NdeII GATC 5 cut(s) 451, 493, 520, 629, 980
NheI GCTAGC 1 cut(s) 155
NlaIV GGNNCC 1 cut(s) 1614
NmuCI GTSAC 4 cut(s) 193, 712, 820, 929
NsiI ATGCAT 1 cut(s) 1249
NspI RCATGY 2 cut(s) 1417, 1425
NspV TTCGAA 1 cut(s) 174
OliI CACNNNNGTG 1 cut(s) 710
PaeI GCATGC 1 cut(s) 1417
PagI TCATGA 1 cut(s) 1054
PaqCI CACCTGC 1 cut(s) 119
PasI CCCWGGG 1 cut(s) 503
PceI AGGCCT 1 cut(s) 288
PdmI GAANNNNTTC 2 cut(s) 180, 1513
PfeI GAWTC 4 cut(s) 216, 795, 1235, 1360
PflMI CCANNNNNTGG 1 cut(s) 503
PinAI ACCGGT 1 cut(s) 1562
PkrI GCNGC 5 cut(s) 298, 315, 994, 1081, 1528
PleI GAGTC 3 cut(s) 53, 139, 1321
PpsI GAGTC 3 cut(s) 53, 139, 1321
PsiI TTATAA 1 cut(s) 777
Psp124BI GAGCTC 1 cut(s) 1668
Psp6I CCWGG 7 cut(s) 283, 288, 502, 595, 782, 970, 1497
PspGI CCWGG 7 cut(s) 283, 288, 502, 595, 782, 970, 1497
PspN4I GGNNCC 1 cut(s) 1614
PspPI GGNCC 5 cut(s) 113, 974, 1115, 1133, 1672
PsuI RGATCY 1 cut(s) 520
RsaI GTAC 4 cut(s) 962, 1063, 1129, 1561
RsaNI GTAC 4 cut(s) 961, 1062, 1128, 1560
RseI CAYNNNNRTG 1 cut(s) 710
SacI GAGCTC 1 cut(s) 1668
SaqAI TTAA 9 cut(s) 240, 327, 750, 920, 1023, 1100, 1355, 1385, 1595
SatI GCNGC 5 cut(s) 297, 314, 993, 1080, 1527
Sau3AI GATC 5 cut(s) 451, 493, 520, 629, 980
Sau96I GGNCC 5 cut(s) 113, 974, 1115, 1133, 1672
ScaI AGTACT 1 cut(s) 1063
SchI GAGTC 3 cut(s) 53, 139, 1321
ScrFI CCNGG 8 cut(s) 285, 290, 476, 504, 597, 784, 972, 1499
SduI GDGCHC 2 cut(s) 1317, 1668
SexAI ACCWGGT 2 cut(s) 595, 782
SfaNI GCATC 5 cut(s) 283, 325, 397, 1060, 1363
SfcI CTRYAG 1 cut(s) 1584
SfuI TTCGAA 1 cut(s) 174
SinI GGWCC 3 cut(s) 974, 1115, 1133
SmiMI CAYNNNNRTG 1 cut(s) 710
SmlI CTYRAG 2 cut(s) 117, 1667
SmoI CTYRAG 2 cut(s) 117, 1667
SpeI ACTAGT 2 cut(s) 190, 1538
SphI GCATGC 1 cut(s) 1417
SseBI AGGCCT 1 cut(s) 288
SsiI CCGC 2 cut(s) 977, 1661
SspMI CTAG 7 cut(s) 156, 191, 357, 459, 884, 1506, 1539
SstI GAGCTC 1 cut(s) 1668
StuI AGGCCT 1 cut(s) 288
StyD4I CCNGG 8 cut(s) 283, 288, 474, 502, 595, 782, 970, 1497
StyI CCWWGG 1 cut(s) 356
TaaI ACNGT 4 cut(s) 17, 880, 1061, 1585
TaiI ACGT 3 cut(s) 108, 200, 1516
TaqI TCGA 4 cut(s) 174, 319, 336, 738
TatI WGTACW 1 cut(s) 1061
TfiI GAWTC 4 cut(s) 216, 795, 1235, 1360
Tru1I TTAA 9 cut(s) 240, 327, 750, 920, 1023, 1100, 1355, 1385, 1595
Tru9I TTAA 9 cut(s) 240, 327, 750, 920, 1023, 1100, 1355, 1385, 1595
TscAI CASTG 1 cut(s) 1536
TseFI GTSAC 4 cut(s) 193, 712, 820, 929
TseI GCWGC 5 cut(s) 296, 313, 992, 1079, 1526
Tsp45I GTSAC 4 cut(s) 193, 712, 820, 929
TspDTI ATGAA 3 cut(s) 208, 405, 657
TspRI CASTG 1 cut(s) 1536
Van91I CCANNNNNTGG 1 cut(s) 503
VneI GTGCAC 1 cut(s) 1313
VpaK11BI GGWCC 3 cut(s) 974, 1115, 1133
XagI CCTNNNNNAGG 1 cut(s) 860
XapI RAATTY 2 cut(s) 169, 1485
XceI RCATGY 2 cut(s) 1417, 1425
XcmI CCANNNNNNNNNTGG 1 cut(s) 1433
XmaJI CCTAGG 1 cut(s) 356
XmiI GTMKAC 1 cut(s) 1587
XmnI GAANNNNTTC 2 cut(s) 180, 1513
XspI CTAG 7 cut(s) 156, 191, 357, 459, 884, 1506, 1539
ZrmI AGTACT 1 cut(s) 1063
Zsp2I ATGCAT 1 cut(s) 1249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.