MD10G1011100.v1.1

Small RNA degrading nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0003406
Physical Location & Seq
Reverse (-)
1550894 .. 1558914
8021 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1011100.v1.1.491

Sequence Viewer

Length: 1710 bp
ATGCCTTCTTCCCCCATTAAAGTCGAAACTTTAGCCCCGGAGGAAGAAGTGAGCTCAAGCAGCAGCAAAAATGATAGCAACTGCTCCACTTTCTTCGATGTCCACGGCCCTCAAAGTAAAGCAGAGGTGGTTTTTAAGACATCGGAAGCCAACACAACTCTGAATTTGCAAGAGGTTCAAGAACTAGTGACGTGGGTGCTTGCTGAAGGATTCATGCCTTCATGGGTTTTTATTAAGAACAAGCCCCTCATTCCCAAAGTGGTTATGCTTTATGTCCCAGGCCTGGACGCAGCATTGTATTTATCACATCATCGACTTCTTAAGAGTCTCAAGGCATTATGTGGCAAGCCAAGGCCAGTTTTAGCTCTCAGTTGTATGTCAGATGGGATGCAGACGATTGATGCACTCCTGATGAGCAAAGTGAAAAGAAAGAGGGAAGAGAGTAGTTCAACTGTGGAGAAATCTAACCTGGCGTCTAAACAAGATAATCTATCTTCTGTGGAACTCATGAAAGATATACCTTTCCCTATTACATTTTACACTCTTACAACAAAACAACTTGAAGAGAATGGATATTGCTTCAATTTGCCAGGTTTTCTTTCTACTCTTCTGGCCCCTTCAGGATCGTCTCACTATCAAATGTTGGCACTTGATTGTGAGATGTGCGTCACAAGTGAGGGTTTTGAGCTAACAAGAGCAACGCTGGTGACTATGAAAGGAGATGTTGTGCTAGATAAATTGGTCAAACCAACAAATCCTATCACTGATTATAATACCAGGTTTAGTGGAATTACAAGTGAGATGTTAGAGGGGGTGACTACAAGTCTTAAGGACATCCAGGAGGACTTTCTAAAGCTGGTTTACAAGGAGACCATCGTAGTGGGCCATTCGTTGGAAAATGACTTGTTAGCATTAAAAATCTCCCACAATCTAGTGATCGATACTGCAGTGTTGTACAAGTACCCCCGTGGTAGGTCTTACAAACCTAAACTTCGTGATCTTGCCCGGAAGTTTCTTTCTAAAGAGATACAGCAGTCAGGGCTTGGCCATGACAGTATTGAAGATGCAAGAGCTACTATGGACCTGGCACTTTTAAAGATTAGACATGGACCCGACTTTGGTACAGCACCATCACTGATACGGAAAAGGCTCCTAACTGTTTTGAGTGAAAGCGGAAAGACATCCTCTATCATTGATGATATTTCCATTGTGAAGCGTCATGCCTCTGAATTATCCCATGCAGTTCCTGTGTTCTCGGATGATGAAGCTCTTTCCAAAGCTCAAAAGGAGGCAAGGAATGAGAAAGTGCATTTCGTCTGGACCCAGTTTTCTGAATTGAATCGTTATTTCACGAAACAAGCAGAAGATGCGGAGAAATTAGATGGAAAAATTGCTGAGTTGATATCCTTGCTTACATGCCATAAGAACTCTGGAAATAAAAAAAGTATCAGCTGCAGCTTAACGTCCGAACTAAAGGAAATTCTTATGCGACTGGATACTAGAATACGTACCTTGTATGCCGCATTGCCTGCTAATGCCATGCTTATTGTCTGCACTGGTCATGGAGATATAGCAACTGTCTCTAGATTAAGGAAAATGCTATTGGATCGTACTGAAACAACAATGTGCCGGGAGAGCATTGTAAAGGTATTGGAAGAGCTCCAAGCCCAAGCTGAGGTAGCATTGTGTTTTGTTGGTGTGAAGAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
RNase_T

Protein Analysis

570

Amino Acids

63.27

Weight (kDa)

7.19

Isoelectric Point (pI)

39.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_T PF00929 216 - 362 3.7e-13 Exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 771
AccB7I CCANNNNNTGG 1 cut(s) 892
AciI CCGC 3 cut(s) 1173, 1370, 1521
AclWI GGATC 2 cut(s) 631, 1614
AcoI YGGCCR 1 cut(s) 1045
AcsI RAATTY 2 cut(s) 163, 1479
AcuI CTGAAG 2 cut(s) 225, 603
AcyI GRCGYC 1 cut(s) 473
AfaI GTAC 5 cut(s) 956, 962, 1123, 1510, 1612
AfiI CCNNNNNNNGG 5 cut(s) 283, 892, 972, 1118, 1675
AflII CTTAAG 2 cut(s) 320, 827
AgsI TTSAA 6 cut(s) 179, 450, 563, 583, 1061, 1339
AhdI GACNNNNNGTC 1 cut(s) 822
AhlI ACTAGT 1 cut(s) 184
AjiI CACGTC 1 cut(s) 192
AjnI CCWGG 7 cut(s) 277, 282, 468, 589, 776, 837, 1083
AjuI GAANNNNNNNTTGG 2 cut(s) 1586, 1618
Alw21I GWGCWC 2 cut(s) 56, 1662
Alw26I GTCTC 4 cut(s) 332, 633, 863, 1585
AlwI GGATC 2 cut(s) 631, 1614
AlwNI CAGNNNCTG 1 cut(s) 1247
AoxI GGCC 6 cut(s) 106, 280, 353, 612, 883, 1045
ApeKI GCWGC 5 cut(s) 60, 63, 290, 1452, 1455
ApoI RAATTY 2 cut(s) 163, 1479
AspS9I GGNCC 6 cut(s) 107, 613, 883, 1081, 1109, 1320
AsuC2I CCSGG 3 cut(s) 38, 1006, 1631
AsuHPI GGTGA 2 cut(s) 718, 826
AvaII GGWCC 3 cut(s) 1081, 1109, 1320
BaeI ACNNNNGTAYC 4 cut(s) 933, 966, 1019, 1052
BalI TGGCCA 1 cut(s) 1047
BanII GRGCYC 2 cut(s) 56, 1662
Bbv12I GWGCWC 2 cut(s) 56, 1662
BbvCI CCTCAGC 1 cut(s) 1674
BbvI GCAGC 5 cut(s) 72, 75, 302, 1439, 1467
BccI CCATC 4 cut(s) 377, 881, 1138, 1376
BceAI ACGGC 1 cut(s) 121
BciT130I CCWGG 7 cut(s) 279, 284, 470, 591, 778, 839, 1085
BciVI GTATCC 1 cut(s) 1489
BcnI CCSGG 3 cut(s) 38, 1006, 1631
BcoDI GTCTC 4 cut(s) 332, 633, 863, 1585
BcuI ACTAGT 1 cut(s) 184
BfaI CTAG 5 cut(s) 185, 731, 932, 1500, 1584
BfmI CTRYAG 2 cut(s) 945, 1453
BfrI CTTAAG 2 cut(s) 320, 827
BfuI GTATCC 1 cut(s) 1489
BisI GCNGC 6 cut(s) 61, 64, 291, 1453, 1456, 1521
BlsI GCNGC 6 cut(s) 62, 65, 292, 1454, 1457, 1522
Bme18I GGWCC 3 cut(s) 1081, 1109, 1320
BmeRI GACNNNNNGTC 1 cut(s) 822
BmgBI CACGTC 1 cut(s) 192
BmgT120I GGNCC 6 cut(s) 107, 613, 883, 1081, 1109, 1320
BmiI GGNNCC 4 cut(s) 615, 1111, 1151, 1322
BmrI ACTGGG 1 cut(s) 1318
BmsI GCATC 4 cut(s) 378, 391, 1054, 1357
BmuI ACTGGG 1 cut(s) 1318
Bpu10I CCTNAGC 1 cut(s) 1674
BpuEI CTTGAG 2 cut(s) 40, 314
BpuMI CCSGG 3 cut(s) 38, 1006, 1631
Bsa29I ATCGAT 1 cut(s) 939
BsaAI YACGTR 1 cut(s) 1508
BsaHI GRCGYC 1 cut(s) 473
BsaI GGTCTC 1 cut(s) 863
BsaJI CCNNGG 5 cut(s) 36, 103, 277, 350, 967
BsaXI ACNNNNNCTCC 2 cut(s) 711, 741
Bsc4I CCNNNNNNNGG 5 cut(s) 283, 892, 972, 1118, 1675
Bse1I ACTGG 4 cut(s) 356, 1324, 1497, 1561
Bse3DI GCAATG 1 cut(s) 1523
BseBI CCWGG 7 cut(s) 279, 284, 470, 591, 778, 839, 1085
BseCI ATCGAT 1 cut(s) 939
BseDI CCNNGG 5 cut(s) 36, 103, 277, 350, 967
BseGI GGATG 4 cut(s) 393, 834, 1181, 1264
BseLI CCNNNNNNNGG 5 cut(s) 283, 892, 972, 1118, 1675
BseMI GCAATG 1 cut(s) 1523
BseMII CTCAG 3 cut(s) 382, 1386, 1665
BseNI ACTGG 4 cut(s) 356, 1324, 1497, 1561
BseXI GCAGC 5 cut(s) 72, 75, 302, 1439, 1467
BsgI GTGCAG 1 cut(s) 1537
BshFI GGCC 6 cut(s) 108, 282, 355, 614, 885, 1047
BshVI ATCGAT 1 cut(s) 939
BsiHKAI GWGCWC 2 cut(s) 56, 1662
BsiSI CCGG 3 cut(s) 38, 1006, 1630
BslFI GGGAC 1 cut(s) 260
BslI CCNNNNNNNGG 5 cut(s) 283, 892, 972, 1118, 1675
BsmAI GTCTC 4 cut(s) 332, 633, 863, 1585
BsmBI CGTCTC 1 cut(s) 633
BsmFI GGGAC 1 cut(s) 260
BsnI GGCC 6 cut(s) 108, 282, 355, 614, 885, 1047
Bso31I GGTCTC 1 cut(s) 863
Bsp1286I GDGCHC 2 cut(s) 56, 1662
Bsp1407I TGTACA 1 cut(s) 954
Bsp143I GATC 4 cut(s) 623, 936, 997, 1606
BspACI CCGC 3 cut(s) 1173, 1370, 1521
BspANI GGCC 6 cut(s) 108, 282, 355, 614, 885, 1047
BspCNI CTCAG 3 cut(s) 381, 1387, 1666
BspDI ATCGAT 1 cut(s) 939
BspHI TCATGA 1 cut(s) 507
BspLI GGNNCC 4 cut(s) 615, 1111, 1151, 1322
BspMAI CTGCAG 2 cut(s) 949, 1457
BspPI GGATC 2 cut(s) 631, 1614
BspQI GCTCTTC 2 cut(s) 1650, 1697
BspTI CTTAAG 2 cut(s) 320, 827
BspTNI GGTCTC 1 cut(s) 863
BsrDI GCAATG 1 cut(s) 1523
BsrGI TGTACA 1 cut(s) 954
BsrI ACTGG 4 cut(s) 356, 1324, 1497, 1561
BssECI CCNNGG 5 cut(s) 36, 103, 277, 350, 967
BssMI GATC 4 cut(s) 623, 936, 997, 1606
BssNI GRCGYC 1 cut(s) 473
BssT1I CCWWGG 1 cut(s) 350
Bst2UI CCWGG 7 cut(s) 279, 284, 470, 591, 778, 839, 1085
Bst4CI ACNGT 4 cut(s) 454, 1055, 1159, 1579
Bst6I CTCTTC 5 cut(s) 432, 558, 612, 1650, 1697
BstACI GRCGYC 1 cut(s) 473
BstAFI CTTAAG 2 cut(s) 320, 827
BstAPI GCANNNNNTGC 2 cut(s) 1367, 1529
BstAUI TGTACA 1 cut(s) 954
BstBAI YACGTR 1 cut(s) 1508
BstC8I GCNNGC 3 cut(s) 201, 347, 1530
BstDEI CTNAG 3 cut(s) 368, 1395, 1674
BstDSI CCRYGG 2 cut(s) 103, 967
BstF5I GGATG 4 cut(s) 393, 834, 1181, 1264
BstKTI GATC 4 cut(s) 626, 939, 1000, 1609
BstMAI GTCTC 4 cut(s) 332, 633, 863, 1585
BstMBI GATC 4 cut(s) 623, 936, 997, 1606
BstMWI GCNNNNNNNGC 6 cut(s) 60, 1039, 1367, 1529, 1635, 1679
BstNI CCWGG 7 cut(s) 279, 284, 470, 591, 778, 839, 1085
BstNSI RCATGY 1 cut(s) 1419
BstSFI CTRYAG 2 cut(s) 945, 1453
BstSNI TACGTA 1 cut(s) 1508
BstV1I GCAGC 5 cut(s) 72, 75, 302, 1439, 1467
BstXI CCANNNNNNTGG 1 cut(s) 880
Bsu15I ATCGAT 1 cut(s) 939
BsuI GTATCC 1 cut(s) 1489
BsuRI GGCC 6 cut(s) 108, 282, 355, 614, 885, 1047
BsuTUI ATCGAT 1 cut(s) 939
BtgI CCRYGG 2 cut(s) 103, 967
BtrI CACGTC 1 cut(s) 192
BtsCI GGATG 4 cut(s) 393, 834, 1181, 1264
BtsI GCAGTG 1 cut(s) 954
BtsIMutI CAGTG 4 cut(s) 762, 954, 1133, 1554
Cac8I GCNNGC 3 cut(s) 201, 347, 1530
CaiI CAGNNNCTG 1 cut(s) 1247
CciI TCATGA 1 cut(s) 507
Cfr13I GGNCC 6 cut(s) 107, 613, 883, 1081, 1109, 1320
ClaI ATCGAT 1 cut(s) 939
CseI GACGC 4 cut(s) 296, 462, 655, 1205
CsiI ACCWGGT 1 cut(s) 776
Csp6I GTAC 5 cut(s) 955, 961, 1122, 1509, 1611
CviQI GTAC 5 cut(s) 955, 961, 1122, 1509, 1611
DdeI CTNAG 3 cut(s) 368, 1395, 1674
DpnI GATC 4 cut(s) 625, 938, 999, 1608
DpnII GATC 4 cut(s) 623, 936, 997, 1606
DraI TTTAAA 1 cut(s) 1095
DriI GACNNNNNGTC 1 cut(s) 822
EaeI YGGCCR 1 cut(s) 1045
Eam1104I CTCTTC 5 cut(s) 432, 558, 612, 1650, 1697
Eam1105I GACNNNNNGTC 1 cut(s) 822
EarI CTCTTC 5 cut(s) 432, 558, 612, 1650, 1697
Ecl136II GAGCTC 2 cut(s) 54, 1660
Eco105I TACGTA 1 cut(s) 1508
Eco130I CCWWGG 1 cut(s) 350
Eco147I AGGCCT 1 cut(s) 282
Eco24I GRGCYC 2 cut(s) 56, 1662
Eco31I GGTCTC 1 cut(s) 863
Eco32I GATATC 1 cut(s) 1404
Eco47I GGWCC 3 cut(s) 1081, 1109, 1320
Eco53kI GAGCTC 2 cut(s) 54, 1660
Eco57I CTGAAG 2 cut(s) 225, 603
EcoICRI GAGCTC 2 cut(s) 54, 1660
EcoRII CCWGG 7 cut(s) 277, 282, 468, 589, 776, 837, 1083
EcoRV GATATC 1 cut(s) 1404
EcoT14I CCWWGG 1 cut(s) 350
EcoT38I GRGCYC 2 cut(s) 56, 1662
ErhI CCWWGG 1 cut(s) 350
Esp3I CGTCTC 1 cut(s) 633
FaqI GGGAC 1 cut(s) 260
Fnu4HI GCNGC 6 cut(s) 61, 64, 291, 1453, 1456, 1521
FokI GGATG 4 cut(s) 400, 821, 1168, 1271
FriOI GRGCYC 2 cut(s) 56, 1662
Fsp4HI GCNGC 6 cut(s) 61, 64, 291, 1453, 1456, 1521
FspBI CTAG 5 cut(s) 185, 731, 932, 1500, 1584
GluI GCNGC 6 cut(s) 61, 64, 291, 1453, 1456, 1521
HaeIII GGCC 6 cut(s) 108, 282, 355, 614, 885, 1047
HapII CCGG 3 cut(s) 38, 1006, 1630
HgaI GACGC 4 cut(s) 296, 462, 655, 1205
Hin1I GRCGYC 1 cut(s) 473
HinfI GANTC 3 cut(s) 210, 325, 1339
HpaII CCGG 3 cut(s) 38, 1006, 1630
HphI GGTGA 2 cut(s) 718, 826
Hpy166II GTNNAC 2 cut(s) 103, 862
Hpy188I TCNGA 7 cut(s) 145, 162, 382, 1228, 1258, 1333, 1468
Hpy188III TCNNGA 9 cut(s) 179, 409, 508, 621, 995, 1318, 1351, 1431, 1584
Hpy8I GTNNAC 2 cut(s) 103, 862
HpyAV CCTTC 4 cut(s) 15, 200, 228, 627
HpyCH4III ACNGT 4 cut(s) 454, 1055, 1159, 1579
HpyCH4IV ACGT 3 cut(s) 191, 1463, 1507
HpyCH4V TGCA 9 cut(s) 169, 391, 404, 947, 1067, 1241, 1309, 1455, 1554
HpyF10VI GCNNNNNNNGC 6 cut(s) 60, 1039, 1367, 1529, 1635, 1679
HpyF3I CTNAG 3 cut(s) 368, 1395, 1674
HpySE526I ACGT 3 cut(s) 191, 1463, 1507
Hsp92I GRCGYC 1 cut(s) 473
Kzo9I GATC 4 cut(s) 623, 936, 997, 1606
LguI GCTCTTC 2 cut(s) 1650, 1697
LmnI GCTCC 3 cut(s) 89, 1155, 1665
Lsp1109I GCAGC 5 cut(s) 72, 75, 302, 1439, 1467
LweI GCATC 4 cut(s) 378, 391, 1054, 1357
MabI ACCWGGT 1 cut(s) 776
MaeI CTAG 5 cut(s) 185, 731, 932, 1500, 1584
MaeII ACGT 3 cut(s) 191, 1463, 1507
MaeIII GTNAC 4 cut(s) 187, 667, 706, 814
MalI GATC 4 cut(s) 625, 938, 999, 1608
MboI GATC 4 cut(s) 623, 936, 997, 1606
MboII GAAGA 9 cut(s) 56, 85, 449, 486, 575, 599, 1073, 1376, 1667
MhlI GDGCHC 2 cut(s) 56, 1662
MlsI TGGCCA 1 cut(s) 1047
MluCI AATT 9 cut(s) 163, 583, 737, 789, 1229, 1334, 1376, 1389, 1479
MluNI TGGCCA 1 cut(s) 1047
MlyI GAGTC 1 cut(s) 334
MmeI TCCRAC 1 cut(s) 873
Mox20I TGGCCA 1 cut(s) 1047
MscI TGGCCA 1 cut(s) 1047
MseI TTAA 9 cut(s) 18, 135, 234, 321, 828, 914, 1094, 1460, 1589
MslI CAYNNNNRTG 1 cut(s) 878
Msp20I TGGCCA 1 cut(s) 1047
MspA1I CMGCKG 1 cut(s) 1452
MspCI CTTAAG 2 cut(s) 320, 827
MspI CCGG 3 cut(s) 38, 1006, 1630
MvaI CCWGG 7 cut(s) 279, 284, 470, 591, 778, 839, 1085
MwoI GCNNNNNNNGC 6 cut(s) 60, 1039, 1367, 1529, 1635, 1679
NciI CCSGG 3 cut(s) 38, 1006, 1631
NdeII GATC 4 cut(s) 623, 936, 997, 1606
NlaIV GGNNCC 4 cut(s) 615, 1111, 1151, 1322
NmuCI GTSAC 4 cut(s) 187, 667, 706, 814
NspI RCATGY 1 cut(s) 1419
PagI TCATGA 1 cut(s) 507
PceI AGGCCT 1 cut(s) 282
PciSI GCTCTTC 2 cut(s) 1650, 1697
PfeI GAWTC 2 cut(s) 210, 1339
PflMI CCANNNNNTGG 1 cut(s) 892
PfoI TCCNGGA 1 cut(s) 837
PkrI GCNGC 6 cut(s) 62, 65, 292, 1454, 1457, 1522
PleI GAGTC 1 cut(s) 333
PpsI GAGTC 1 cut(s) 333
Ppu21I YACGTR 1 cut(s) 1508
PsiI TTATAA 1 cut(s) 771
Psp124BI GAGCTC 2 cut(s) 56, 1662
Psp6I CCWGG 7 cut(s) 277, 282, 468, 589, 776, 837, 1083
PspGI CCWGG 7 cut(s) 277, 282, 468, 589, 776, 837, 1083
PspN4I GGNNCC 4 cut(s) 615, 1111, 1151, 1322
PspPI GGNCC 6 cut(s) 107, 613, 883, 1081, 1109, 1320
PstI CTGCAG 2 cut(s) 949, 1457
PstNI CAGNNNCTG 1 cut(s) 1247
PvuII CAGCTG 1 cut(s) 1452
RsaI GTAC 5 cut(s) 956, 962, 1123, 1510, 1612
RsaNI GTAC 5 cut(s) 955, 961, 1122, 1509, 1611
RseI CAYNNNNRTG 1 cut(s) 878
SacI GAGCTC 2 cut(s) 56, 1662
SapI GCTCTTC 2 cut(s) 1650, 1697
SaqAI TTAA 9 cut(s) 18, 135, 234, 321, 828, 914, 1094, 1460, 1589
SatI GCNGC 6 cut(s) 61, 64, 291, 1453, 1456, 1521
Sau3AI GATC 4 cut(s) 623, 936, 997, 1606
Sau96I GGNCC 6 cut(s) 107, 613, 883, 1081, 1109, 1320
SchI GAGTC 1 cut(s) 334
SduI GDGCHC 2 cut(s) 56, 1662
SexAI ACCWGGT 1 cut(s) 776
SfaNI GCATC 4 cut(s) 378, 391, 1054, 1357
SfcI CTRYAG 2 cut(s) 945, 1453
SinI GGWCC 3 cut(s) 1081, 1109, 1320
SmiMI CAYNNNNRTG 1 cut(s) 878
SmlI CTYRAG 4 cut(s) 55, 320, 329, 827
SmoI CTYRAG 4 cut(s) 55, 320, 329, 827
SnaBI TACGTA 1 cut(s) 1508
SpeI ACTAGT 1 cut(s) 184
Sse9I AATT 9 cut(s) 163, 583, 737, 789, 1229, 1334, 1376, 1389, 1479
SseBI AGGCCT 1 cut(s) 282
SsiI CCGC 3 cut(s) 1173, 1370, 1521
SspMI CTAG 5 cut(s) 185, 731, 932, 1500, 1584
SstI GAGCTC 2 cut(s) 56, 1662
StuI AGGCCT 1 cut(s) 282
StyI CCWWGG 1 cut(s) 350
TaaI ACNGT 4 cut(s) 454, 1055, 1159, 1579
TaiI ACGT 3 cut(s) 194, 1466, 1510
TaqI TCGA 4 cut(s) 24, 96, 313, 939
TasI AATT 9 cut(s) 163, 583, 737, 789, 1229, 1334, 1376, 1389, 1479
TatI WGTACW 1 cut(s) 954
TauI GCSGC 1 cut(s) 1523
TfiI GAWTC 2 cut(s) 210, 1339
Tru1I TTAA 9 cut(s) 18, 135, 234, 321, 828, 914, 1094, 1460, 1589
Tru9I TTAA 9 cut(s) 18, 135, 234, 321, 828, 914, 1094, 1460, 1589
TscAI CASTG 4 cut(s) 769, 954, 1140, 1561
TseFI GTSAC 4 cut(s) 187, 667, 706, 814
TseI GCWGC 5 cut(s) 60, 63, 290, 1452, 1455
Tsp45I GTSAC 4 cut(s) 187, 667, 706, 814
TspDTI ATGAA 5 cut(s) 202, 210, 524, 728, 1278
TspGWI ACGGA 1 cut(s) 1156
TspRI CASTG 4 cut(s) 769, 954, 1140, 1561
Van91I CCANNNNNTGG 1 cut(s) 892
Vha464I CTTAAG 2 cut(s) 320, 827
VpaK11BI GGWCC 3 cut(s) 1081, 1109, 1320
XapI RAATTY 2 cut(s) 163, 1479
XbaI TCTAGA 1 cut(s) 1583
XceI RCATGY 1 cut(s) 1419
XcmI CCANNNNNNNNNTGG 1 cut(s) 1427
XspI CTAG 5 cut(s) 185, 731, 932, 1500, 1584
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.