MD05G1011300.v1.1

Small RNA degrading nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0003406
Physical Location & Seq
Reverse (-)
2346389 .. 2353465
7077 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1011300.v1.1.491

Sequence Viewer

Length: 1710 bp
ATGCCTTCTTCCCCCATTAAAGTCGAAACTTTAGCTTCGGAGGAAGAAGTGAGCTCAAGCAGCAACGCAAATGTTAGCAACCGCTCCACTTTTTTCGATGTCTACGGCCCTCAAAGTAAAGCAGAGGTGATTTTTAAGACGCTGGAAACCGACACAACTCTGAATTTGCAAGAGGTTCAAGAACTGGTGACATGGGTGCTTGCTGAAGGTTTCATGCCATCATGGGTTTTTATTAAGAACAAGCCCCTCATTCCCAAAGTGGTTATGCTTTATGTCCCAGGACTGGATGCAGCATTGTACTTAGCACATCGTCGACTTCTTAAGAGTCTCAAAGCATTGTGTAGCAAGCCAAGGCCAGTTTTAGCTCTCAGCTGTATGTCAGATGGGATGCAGACGATTGATGCACTCCTGACGAGCAAAGTGAAAAGAAAGAGGGAAGAAAGTAGTTTAACTGTGGAGAAATCTAACCTGGCATCCAAACAAGATGATCTATCCTCAGTGGACCTCATGAAAGATCTACCTTTCCCTATTACATTTTACACACTTACAACAAAACAACTTGAAGACAATGGATATTGCTTCAATTTGCCAGGTTTTCTTTCTACTCTTCCGGCCCCTTCAGGATCATCCCACTATCAAATGCTGGCACTTGATTGCGAGATGTGTATCACAAGTGAGGGTTTTGAGCTAACAAGAGCAACGCTGGTGACTATGAAAGGAGATGTTGTGCTAGATAAATTGGTTAAACCAACAAATCCTATCACTGATTATAATACCAGGTTTAGCGGAATCACAAGTGAGATGTTAGATGGGGTGACTACAATTCTTAAAGAAATTCAGGAGGACTTTCTAAAGCTGGTTTACAAGGAGACCGTGCTAGTGGGGCATTCCTTGGAAAATGACTTGTTAGCATTAAAAATCTCCCACAATCTAGTGATCGATACTGCAGTGTTGTACAAGTACCCCCGTGGTAGGTCTTACAAATCTAAACTTCGAGATCTTGCCCAGAAATTTCTTTCTAAAGAGATACAGCAGTCAGGGCTTGGCCATGACAGTATTGAAGATGCAAGAGCTGCTATGGACCTGGCACTTTTAAAGATTAGACATGGACCTGACTTTGGTACAGCACCATCACTGGTACGGAAAAGGTTCCTAACTGTTTTGAGTGAGAGCGGAAAGACCTCCTCTATCATTGATGATATTTCCATTGTGAAGCGTCATGCCTCTGAATTATCCCATGCAGTGCCTGTGTTCTCTGATGACGAAGCTCTTTCAAAAGCTCAAAAGGAGGCAAGGAATGAGAAAGTGCAATTCATCTGGACCCAGTTTTCTGAATTGAATCGTTATTTCACTAAACAAGCAGAGGATGCTGAGAAATTGAATGGAAAAATTGCGGAGTTGATATCCTTGCTTACATGTCATAAGAATTCTGGAAATAAAAAAGGTATTAGATGCAGCTTAACGTCTGAACTAAAGGAAATTCTTATGCGACTGGATACTAGAATACATACCTTGTATGCCTCATTGCCCACTAATGCCATGCTTATTGTCTGCACTGGTCATGGAGATATAGCTACTGTCTCTAGATTAAAGAAAATGCTTATGGATCGTAGTGAAACAACAATGTGCCGTGAGAGCATTGTAAAGGTCTTGGAAGAGCTCCAAGCCCAAGCTGAGGTAGCACTGTGTTTTGTTGGTGTGAAGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
RNase_T

Protein Analysis

570

Amino Acids

63.38

Weight (kDa)

7.15

Isoelectric Point (pI)

39.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_T PF00929 216 - 362 2.3e-13 Exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 771
AccBSI CCGCTC 2 cut(s) 84, 1173
AccI GTMKAC 2 cut(s) 102, 313
AciI CCGC 4 cut(s) 82, 786, 1173, 1394
AclWI GGATC 2 cut(s) 631, 1614
AcoI YGGCCR 1 cut(s) 1045
AcsI RAATTY 5 cut(s) 163, 834, 1010, 1426, 1479
AcuI CTGAAG 2 cut(s) 225, 603
AfaI GTAC 5 cut(s) 299, 956, 962, 1123, 1140
AfiI CCNNNNNNNGG 4 cut(s) 283, 972, 1118, 1675
AflII CTTAAG 1 cut(s) 320
AflIII ACRYGT 1 cut(s) 1415
AgsI TTSAA 7 cut(s) 179, 563, 583, 1061, 1275, 1339, 1381
AjnI CCWGG 5 cut(s) 277, 468, 589, 776, 1083
Alw21I GWGCWC 2 cut(s) 56, 1662
Alw26I GTCTC 3 cut(s) 332, 863, 1585
AlwI GGATC 2 cut(s) 631, 1614
AlwNI CAGNNNCTG 1 cut(s) 1247
AoxI GGCC 4 cut(s) 106, 353, 612, 1045
ApeKI GCWGC 4 cut(s) 60, 290, 1073, 1455
ApoI RAATTY 5 cut(s) 163, 834, 1010, 1426, 1479
Asp700I GAANNNNTTC 1 cut(s) 1148
AspS9I GGNCC 6 cut(s) 107, 502, 613, 1081, 1109, 1320
AsuHPI GGTGA 4 cut(s) 139, 199, 718, 826
AvaII GGWCC 4 cut(s) 502, 1081, 1109, 1320
BaeI ACNNNNGTAYC 4 cut(s) 933, 966, 1019, 1052
BalI TGGCCA 1 cut(s) 1047
BanII GRGCYC 2 cut(s) 56, 1662
BbsI GAAGAC 1 cut(s) 570
Bbv12I GWGCWC 2 cut(s) 56, 1662
BbvCI CCTCAGC 1 cut(s) 1674
BbvI GCAGC 4 cut(s) 72, 302, 1060, 1467
BccI CCATC 4 cut(s) 226, 377, 803, 1138
BceAI ACGGC 2 cut(s) 121, 1614
BciT130I CCWGG 5 cut(s) 279, 470, 591, 778, 1085
BciVI GTATCC 1 cut(s) 1489
BcoDI GTCTC 3 cut(s) 332, 863, 1585
BfaI CTAG 5 cut(s) 731, 878, 932, 1500, 1584
BfmI CTRYAG 1 cut(s) 945
BfrI CTTAAG 1 cut(s) 320
BfuI GTATCC 1 cut(s) 1489
BglII AGATCT 2 cut(s) 514, 997
BisI GCNGC 4 cut(s) 61, 291, 1074, 1456
BlsI GCNGC 4 cut(s) 62, 292, 1075, 1457
Bme1390I CCNGG 5 cut(s) 279, 470, 591, 778, 1085
Bme18I GGWCC 4 cut(s) 502, 1081, 1109, 1320
BmgT120I GGNCC 6 cut(s) 107, 502, 613, 1081, 1109, 1320
BmiI GGNNCC 3 cut(s) 615, 1151, 1322
BmrFI CCNGG 5 cut(s) 279, 470, 591, 778, 1085
BmrI ACTGGG 1 cut(s) 1318
BmsI GCATC 7 cut(s) 277, 378, 391, 482, 1054, 1357, 1442
BmuI ACTGGG 1 cut(s) 1318
BpiI GAAGAC 1 cut(s) 570
Bpu10I CCTNAGC 1 cut(s) 1674
BpuEI CTTGAG 1 cut(s) 40
Bsa29I ATCGAT 1 cut(s) 939
BsaBI GATNNNNATC 1 cut(s) 665
BsaI GGTCTC 1 cut(s) 863
BsaJI CCNNGG 4 cut(s) 277, 350, 891, 967
BsaXI ACNNNNNCTCC 2 cut(s) 711, 741
Bsc4I CCNNNNNNNGG 4 cut(s) 283, 972, 1118, 1675
Bse1I ACTGG 7 cut(s) 189, 288, 356, 1140, 1324, 1497, 1561
Bse3DI GCAATG 1 cut(s) 1523
Bse8I GATNNNNATC 1 cut(s) 665
BseBI CCWGG 5 cut(s) 279, 470, 591, 778, 1085
BseCI ATCGAT 1 cut(s) 939
BseDI CCNNGG 4 cut(s) 277, 350, 891, 967
BseGI GGATG 5 cut(s) 292, 393, 473, 626, 1372
BseJI GATNNNNATC 1 cut(s) 665
BseLI CCNNNNNNNGG 4 cut(s) 283, 972, 1118, 1675
BseMI GCAATG 1 cut(s) 1523
BseMII CTCAG 4 cut(s) 382, 510, 1362, 1665
BseNI ACTGG 7 cut(s) 189, 288, 356, 1140, 1324, 1497, 1561
BseRI GAGGAG 1 cut(s) 1174
BseXI GCAGC 4 cut(s) 72, 302, 1060, 1467
BsgI GTGCAG 1 cut(s) 1537
BshFI GGCC 4 cut(s) 108, 355, 614, 1047
BshVI ATCGAT 1 cut(s) 939
BsiHKAI GWGCWC 2 cut(s) 56, 1662
BsiSI CCGG 1 cut(s) 611
BslFI GGGAC 1 cut(s) 260
BslI CCNNNNNNNGG 4 cut(s) 283, 972, 1118, 1675
BsmAI GTCTC 3 cut(s) 332, 863, 1585
BsmFI GGGAC 1 cut(s) 260
BsmI GAATGC 1 cut(s) 886
BsnI GGCC 4 cut(s) 108, 355, 614, 1047
Bso31I GGTCTC 1 cut(s) 863
Bsp1286I GDGCHC 2 cut(s) 56, 1662
Bsp1407I TGTACA 1 cut(s) 954
Bsp143I GATC 6 cut(s) 487, 514, 623, 936, 997, 1606
BspACI CCGC 4 cut(s) 82, 786, 1173, 1394
BspANI GGCC 4 cut(s) 108, 355, 614, 1047
BspCNI CTCAG 4 cut(s) 381, 509, 1363, 1666
BspDI ATCGAT 1 cut(s) 939
BspHI TCATGA 1 cut(s) 507
BspLI GGNNCC 3 cut(s) 615, 1151, 1322
BspMAI CTGCAG 1 cut(s) 949
BspPI GGATC 2 cut(s) 631, 1614
BspQI GCTCTTC 1 cut(s) 1650
BspTI CTTAAG 1 cut(s) 320
BspTNI GGTCTC 1 cut(s) 863
BsrBI CCGCTC 2 cut(s) 84, 1173
BsrDI GCAATG 1 cut(s) 1523
BsrGI TGTACA 1 cut(s) 954
BsrI ACTGG 7 cut(s) 189, 288, 356, 1140, 1324, 1497, 1561
BssECI CCNNGG 4 cut(s) 277, 350, 891, 967
BssMI GATC 6 cut(s) 487, 514, 623, 936, 997, 1606
BssT1I CCWWGG 2 cut(s) 350, 891
Bst2UI CCWGG 5 cut(s) 279, 470, 591, 778, 1085
Bst4CI ACNGT 6 cut(s) 454, 874, 1055, 1159, 1579, 1686
Bst6I CTCTTC 3 cut(s) 612, 1650, 1697
BstAFI CTTAAG 1 cut(s) 320
BstAPI GCANNNNNTGC 2 cut(s) 1073, 1367
BstAUI TGTACA 1 cut(s) 954
BstC8I GCNNGC 3 cut(s) 201, 347, 645
BstDEI CTNAG 5 cut(s) 301, 368, 496, 1371, 1674
BstDSI CCRYGG 1 cut(s) 967
BstF5I GGATG 5 cut(s) 292, 393, 473, 626, 1372
BstKTI GATC 6 cut(s) 490, 517, 626, 939, 1000, 1609
BstMAI GTCTC 3 cut(s) 332, 863, 1585
BstMBI GATC 6 cut(s) 487, 514, 623, 936, 997, 1606
BstMWI GCNNNNNNNGC 7 cut(s) 60, 883, 1039, 1073, 1367, 1635, 1679
BstNI CCWGG 5 cut(s) 279, 470, 591, 778, 1085
BstNSI RCATGY 1 cut(s) 1419
BstSCI CCNGG 5 cut(s) 277, 468, 589, 776, 1083
BstSFI CTRYAG 1 cut(s) 945
BstV1I GCAGC 4 cut(s) 72, 302, 1060, 1467
BstV2I GAAGAC 1 cut(s) 570
BstX2I RGATCY 2 cut(s) 514, 997
BstYI RGATCY 2 cut(s) 514, 997
Bsu15I ATCGAT 1 cut(s) 939
BsuI GTATCC 1 cut(s) 1489
BsuRI GGCC 4 cut(s) 108, 355, 614, 1047
BsuTUI ATCGAT 1 cut(s) 939
BtgI CCRYGG 1 cut(s) 967
BtsCI GGATG 5 cut(s) 292, 393, 473, 626, 1372
BtsI GCAGTG 2 cut(s) 954, 1248
BtsIMutI CAGTG 7 cut(s) 504, 762, 954, 1133, 1248, 1554, 1682
Cac8I GCNNGC 3 cut(s) 201, 347, 645
CaiI CAGNNNCTG 1 cut(s) 1247
CciI TCATGA 1 cut(s) 507
Cfr13I GGNCC 6 cut(s) 107, 502, 613, 1081, 1109, 1320
ClaI ATCGAT 1 cut(s) 939
CseI GACGC 2 cut(s) 148, 1205
CsiI ACCWGGT 1 cut(s) 776
Csp6I GTAC 5 cut(s) 298, 955, 961, 1122, 1139
CviQI GTAC 5 cut(s) 298, 955, 961, 1122, 1139
DdeI CTNAG 5 cut(s) 301, 368, 496, 1371, 1674
DpnI GATC 6 cut(s) 489, 516, 625, 938, 999, 1608
DpnII GATC 6 cut(s) 487, 514, 623, 936, 997, 1606
DraI TTTAAA 1 cut(s) 1095
EaeI YGGCCR 1 cut(s) 1045
Eam1104I CTCTTC 3 cut(s) 612, 1650, 1697
EarI CTCTTC 3 cut(s) 612, 1650, 1697
Ecl136II GAGCTC 2 cut(s) 54, 1660
Eco130I CCWWGG 2 cut(s) 350, 891
Eco24I GRGCYC 2 cut(s) 56, 1662
Eco31I GGTCTC 1 cut(s) 863
Eco32I GATATC 1 cut(s) 1404
Eco47I GGWCC 4 cut(s) 502, 1081, 1109, 1320
Eco53kI GAGCTC 2 cut(s) 54, 1660
Eco57I CTGAAG 2 cut(s) 225, 603
EcoICRI GAGCTC 2 cut(s) 54, 1660
EcoRI GAATTC 1 cut(s) 1426
EcoRII CCWGG 5 cut(s) 277, 468, 589, 776, 1083
EcoRV GATATC 1 cut(s) 1404
EcoT14I CCWWGG 2 cut(s) 350, 891
EcoT38I GRGCYC 2 cut(s) 56, 1662
ErhI CCWWGG 2 cut(s) 350, 891
FaqI GGGAC 1 cut(s) 260
FblI GTMKAC 2 cut(s) 102, 313
Fnu4HI GCNGC 4 cut(s) 61, 291, 1074, 1456
FokI GGATG 5 cut(s) 299, 400, 460, 613, 1379
FriOI GRGCYC 2 cut(s) 56, 1662
Fsp4HI GCNGC 4 cut(s) 61, 291, 1074, 1456
FspBI CTAG 5 cut(s) 731, 878, 932, 1500, 1584
GluI GCNGC 4 cut(s) 61, 291, 1074, 1456
HaeIII GGCC 4 cut(s) 108, 355, 614, 1047
HapII CCGG 1 cut(s) 611
HgaI GACGC 2 cut(s) 148, 1205
HincII GTYRAC 1 cut(s) 314
HindII GTYRAC 1 cut(s) 314
HinfI GANTC 3 cut(s) 325, 789, 1339
HpaII CCGG 1 cut(s) 611
HphI GGTGA 4 cut(s) 139, 199, 718, 826
Hpy166II GTNNAC 4 cut(s) 103, 314, 502, 862
Hpy188I TCNGA 7 cut(s) 40, 162, 382, 1228, 1258, 1333, 1468
Hpy188III TCNNGA 9 cut(s) 179, 409, 508, 621, 839, 995, 1318, 1431, 1584
Hpy8I GTNNAC 4 cut(s) 103, 314, 502, 862
Hpy99I CGWCG 1 cut(s) 315
HpyAV CCTTC 3 cut(s) 15, 200, 627
HpyCH4III ACNGT 6 cut(s) 454, 874, 1055, 1159, 1579, 1686
HpyCH4IV ACGT 1 cut(s) 1463
HpyF10VI GCNNNNNNNGC 7 cut(s) 60, 883, 1039, 1073, 1367, 1635, 1679
HpyF3I CTNAG 5 cut(s) 301, 368, 496, 1371, 1674
HpySE526I ACGT 1 cut(s) 1463
Kzo9I GATC 6 cut(s) 487, 514, 623, 936, 997, 1606
LguI GCTCTTC 1 cut(s) 1650
LmnI GCTCC 2 cut(s) 89, 1665
Lsp1109I GCAGC 4 cut(s) 72, 302, 1060, 1467
LweI GCATC 7 cut(s) 277, 378, 391, 482, 1054, 1357, 1442
MabI ACCWGGT 1 cut(s) 776
MaeI CTAG 5 cut(s) 731, 878, 932, 1500, 1584
MaeII ACGT 1 cut(s) 1463
MaeIII GTNAC 3 cut(s) 187, 706, 814
MalI GATC 6 cut(s) 489, 516, 625, 938, 999, 1608
MbiI CCGCTC 2 cut(s) 84, 1173
MboI GATC 6 cut(s) 487, 514, 623, 936, 997, 1606
MboII GAAGA 6 cut(s) 56, 449, 575, 599, 1073, 1667
MflI RGATCY 2 cut(s) 514, 997
MhlI GDGCHC 2 cut(s) 56, 1662
MlsI TGGCCA 1 cut(s) 1047
MluNI TGGCCA 1 cut(s) 1047
MlyI GAGTC 1 cut(s) 334
Mox20I TGGCCA 1 cut(s) 1047
MroXI GAANNNNTTC 1 cut(s) 1148
MscI TGGCCA 1 cut(s) 1047
Msp20I TGGCCA 1 cut(s) 1047
MspA1I CMGCKG 1 cut(s) 372
MspCI CTTAAG 1 cut(s) 320
MspI CCGG 1 cut(s) 611
MspR9I CCNGG 5 cut(s) 279, 470, 591, 778, 1085
Mva1269I GAATGC 1 cut(s) 886
MvaI CCWGG 5 cut(s) 279, 470, 591, 778, 1085
MwoI GCNNNNNNNGC 7 cut(s) 60, 883, 1039, 1073, 1367, 1635, 1679
NdeII GATC 6 cut(s) 487, 514, 623, 936, 997, 1606
NlaIV GGNNCC 3 cut(s) 615, 1151, 1322
NmuCI GTSAC 3 cut(s) 187, 706, 814
NspI RCATGY 1 cut(s) 1419
PagI TCATGA 1 cut(s) 507
PciI ACATGT 1 cut(s) 1415
PciSI GCTCTTC 1 cut(s) 1650
PctI GAATGC 1 cut(s) 886
PdmI GAANNNNTTC 1 cut(s) 1148
PfeI GAWTC 2 cut(s) 789, 1339
PkrI GCNGC 4 cut(s) 62, 292, 1075, 1457
PleI GAGTC 1 cut(s) 333
PpsI GAGTC 1 cut(s) 333
PscI ACATGT 1 cut(s) 1415
PsiI TTATAA 1 cut(s) 771
Psp124BI GAGCTC 2 cut(s) 56, 1662
Psp6I CCWGG 5 cut(s) 277, 468, 589, 776, 1083
PspGI CCWGG 5 cut(s) 277, 468, 589, 776, 1083
PspN4I GGNNCC 3 cut(s) 615, 1151, 1322
PspPI GGNCC 6 cut(s) 107, 502, 613, 1081, 1109, 1320
PstI CTGCAG 1 cut(s) 949
PstNI CAGNNNCTG 1 cut(s) 1247
PsuI RGATCY 2 cut(s) 514, 997
PvuII CAGCTG 1 cut(s) 372
RsaI GTAC 5 cut(s) 299, 956, 962, 1123, 1140
RsaNI GTAC 5 cut(s) 298, 955, 961, 1122, 1139
SacI GAGCTC 2 cut(s) 56, 1662
SalI GTCGAC 1 cut(s) 312
SapI GCTCTTC 1 cut(s) 1650
SatI GCNGC 4 cut(s) 61, 291, 1074, 1456
Sau3AI GATC 6 cut(s) 487, 514, 623, 936, 997, 1606
Sau96I GGNCC 6 cut(s) 107, 502, 613, 1081, 1109, 1320
SchI GAGTC 1 cut(s) 334
ScrFI CCNGG 5 cut(s) 279, 470, 591, 778, 1085
SduI GDGCHC 2 cut(s) 56, 1662
SexAI ACCWGGT 1 cut(s) 776
SfaNI GCATC 7 cut(s) 277, 378, 391, 482, 1054, 1357, 1442
SfcI CTRYAG 1 cut(s) 945
SinI GGWCC 4 cut(s) 502, 1081, 1109, 1320
SmlI CTYRAG 2 cut(s) 55, 320
SmoI CTYRAG 2 cut(s) 55, 320
SsiI CCGC 4 cut(s) 82, 786, 1173, 1394
SspMI CTAG 5 cut(s) 731, 878, 932, 1500, 1584
SstI GAGCTC 2 cut(s) 56, 1662
StyD4I CCNGG 5 cut(s) 277, 468, 589, 776, 1083
StyI CCWWGG 2 cut(s) 350, 891
TaaI ACNGT 6 cut(s) 454, 874, 1055, 1159, 1579, 1686
TaiI ACGT 1 cut(s) 1466
TaqI TCGA 5 cut(s) 24, 96, 313, 939, 994
TatI WGTACW 2 cut(s) 297, 954
TfiI GAWTC 2 cut(s) 789, 1339
TscAI CASTG 7 cut(s) 504, 769, 954, 1140, 1248, 1561, 1689
TseFI GTSAC 3 cut(s) 187, 706, 814
TseI GCWGC 4 cut(s) 60, 290, 1073, 1455
Tsp45I GTSAC 3 cut(s) 187, 706, 814
TspDTI ATGAA 4 cut(s) 202, 524, 728, 1303
TspGWI ACGGA 1 cut(s) 1156
TspRI CASTG 7 cut(s) 504, 769, 954, 1140, 1248, 1561, 1689
Vha464I CTTAAG 1 cut(s) 320
VpaK11BI GGWCC 4 cut(s) 502, 1081, 1109, 1320
XapI RAATTY 5 cut(s) 163, 834, 1010, 1426, 1479
XbaI TCTAGA 1 cut(s) 1583
XceI RCATGY 1 cut(s) 1419
XmiI GTMKAC 2 cut(s) 102, 313
XmnI GAANNNNTTC 1 cut(s) 1148
XspI CTAG 5 cut(s) 731, 878, 932, 1500, 1584
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.