Rh6BG224100

Small RNA degrading nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0003406
Physical Location & Seq
Forward (+)
42367602 .. 42379417
11816 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG224100.1

Sequence Viewer

Length: 1713 bp
ATGCCTTCGTCCCACAGTAAATGCGAAGAAGAAGTTTTAGCTACCGAGGAAGACGACTCAAACAGAGACCAGAGCAATGCCAAAAGCTCGAATTTCTATGACGTTTATGGGCCTCAAGCTAAAGCAGGTGTGGAATTTAAGACTCCAGAAGCTACTTCAACTCTGAATTTCGAAGAGGTACAACAACTAGTGACGTGGGTGCTTGCTGATGGATTCATGCCGTCGTGGGTGTTTGTAAAGAACAAGCCCCTCATTCCCAAAGTGGTTATGCTTTATGTCCCAGGCCTAGATGCAGCTCTATACTTATCACAGCATCGACTACTTAAGGGTCTCGAAAAATGTTGTGGAAAACCAAGGCCTGTTTTAGCACTCAGCTATATGTCACATGAAAATCAGACGATTGATGCGCTCCTAACATGCAAACTGAAAAGAAAGAGGGAAGAGAGTGATCCAGCTAGGACAAAATCTAACCAGGAAACTCAACAAGATGATCCATCCTCTGTGGAACTTCTGAAAGATCTACCATTCCCAGTTACATTTTACACTCTTACAACAAAACAACTGGAAGACAATGGATATGTCTTCAATCAACCAGGTTTTCTTTCTACTCTTCCTGCTCCTTCTGGATCATCTCCTTATGAAATGTTAGCACTTGATTGTGAGATGTGTGTCACAGAAGAGGGTTTTGAGCTAACGAGAGCGACGCTTGTGACTATAAAAGGAGAGGTTGTGCTTGATAAATTGGTTAAACCAACAAATGCTATCAAAGATTATAACACCAGGTTTAGTGGAATCACACCTGAGATGTTAGATGGGGTGACTACAAGCCTCAAAGATATTCAGGAGGACTTCTTAAAGCTGGTTTACAAAGAGACCGTCCTAGTTGGCCATTCATTGGAGAATGACTTATTAGCATTGAAAATTGCTCACAATCTCGTGATTGATACAGCAGTATTGTACAAGCACCCTGGTCAACGATCTTACAAATCTGCTCTTAGAGTTCTTGCCAAGAGATATCTTTCTAAAGAGATCCAGCAGTCAGGGAATGGTCATGACAGTACTGAAGATGCAAGAGCTGCTATGGAACTGGCGCTTTTAAAGATTAGACATGGACCTGACTTTGGCACAGCACCATCAGTTTTCCGGAAAAAGCTGCTAAAAGTTTTGAGCGATAATGGAAAGACCACCTCTATCATTGATGATGTTACCATTGTGAAGCGCTATGCCTCTGAATCATCCCATGCATTTCCTGTGGCCTCTGATGAGGAAGCTCTACCTAAAGCTGTAAAGGAGGCAAGGAACGAGAAAGTGCAGTTTGTCTGGACGCAATTCTCAGAATTATATCGCTTTTTAAAGAATCAAGCAGAGGATGCTGAGAAATTAGATGTAAGACTTGCTCAGATGATAAGCTTGCTTACGTGCCATAAGAAGTCTGGAGATAAAAAGGGTATTAGATGGAGTTTGACGTCTGAACTAAAGGAAATTCTTATCCGCGTGGACACTAGAATACGTGCTCTCTATGCTGCACTACCCACTAATGCCATGCTTATCATTTGTACCGGTCATGGAGATATAGCAACTGTAGATAGATTAAACAAAATGCTTTCGGAGCGTGAGGAAACCACAATGTGTCGGGATAGCATTGTAAAGGTCTTAGCGGAGCTCAAGGCCCAAGCTGAGGTAGCTTTATGTTTTGTGGGTGTGAAGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
RNase_T

Protein Analysis

570

Amino Acids

63.71

Weight (kDa)

6.2

Isoelectric Point (pI)

34.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_T PF00929 217 - 363 1.4e-11 Exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 774
AarI CACCTGC 1 cut(s) 116
AatII GACGTC 1 cut(s) 1469
Acc36I ACCTGC 1 cut(s) 116
AccB7I CCANNNNNTGG 1 cut(s) 895
AccII CGCG 1 cut(s) 1494
AccIII TCCGGA 1 cut(s) 1143
AciI CCGC 2 cut(s) 1492, 1658
AclWI GGATC 4 cut(s) 443, 485, 634, 1024
AcoI YGGCCR 1 cut(s) 886
AcsI RAATTY 4 cut(s) 91, 134, 166, 1482
AcuI CTGAAG 1 cut(s) 1083
AcyI GRCGYC 1 cut(s) 1466
AdeI CACNNNGTG 1 cut(s) 1629
AfaI GTAC 4 cut(s) 180, 959, 1060, 1558
AfeI AGCGCT 1 cut(s) 1220
AfiI CCNNNNNNNGG 3 cut(s) 895, 1121, 1678
AflII CTTAAG 1 cut(s) 323
AgeI ACCGGT 1 cut(s) 1559
AgsI TTSAA 3 cut(s) 159, 586, 919
AhlI ACTAGT 1 cut(s) 187
AjiI CACGTC 1 cut(s) 195
AjnI CCWGG 5 cut(s) 280, 471, 592, 779, 967
Alw21I GWGCWC 2 cut(s) 1516, 1665
Alw26I GTCTC 3 cut(s) 60, 335, 866
AlwI GGATC 4 cut(s) 443, 485, 634, 1024
Aor13HI TCCGGA 1 cut(s) 1143
Aor51HI AGCGCT 1 cut(s) 1220
AoxI GGCC 6 cut(s) 110, 283, 356, 886, 1254, 1668
ApeKI GCWGC 4 cut(s) 293, 1076, 1153, 1523
ApoI RAATTY 4 cut(s) 91, 134, 166, 1482
ArsI GACNNNNNNTTYG 2 cut(s) 861, 893
AsiGI ACCGGT 1 cut(s) 1559
AspLEI GCGC 3 cut(s) 409, 1093, 1221
AspS9I GGNCC 3 cut(s) 110, 1112, 1669
AsuHPI GGTGA 1 cut(s) 829
AsuII TTCGAA 1 cut(s) 171
AvaII GGWCC 1 cut(s) 1112
BaeI ACNNNNGTAYC 2 cut(s) 936, 969
BalI TGGCCA 1 cut(s) 888
BanII GRGCYC 1 cut(s) 1665
BauI CACGAG 1 cut(s) 935
BbsI GAAGAC 3 cut(s) 57, 573, 574
Bbv12I GWGCWC 2 cut(s) 1516, 1665
BbvCI CCTCAGC 1 cut(s) 1677
BbvI GCAGC 4 cut(s) 305, 1063, 1140, 1510
BccI CCATC 5 cut(s) 203, 502, 806, 1141, 1449
BceAI ACGGC 1 cut(s) 205
BciT130I CCWGG 5 cut(s) 282, 473, 594, 781, 969
BcoDI GTCTC 3 cut(s) 60, 335, 866
BcuI ACTAGT 1 cut(s) 187
BfaI CTAG 5 cut(s) 188, 287, 456, 881, 1503
BfmI CTRYAG 1 cut(s) 1581
BfoI RGCGCY 2 cut(s) 1094, 1222
BfrI CTTAAG 1 cut(s) 323
BfuAI ACCTGC 1 cut(s) 116
BglII AGATCT 1 cut(s) 517
BisI GCNGC 4 cut(s) 294, 1077, 1154, 1524
BlsI GCNGC 4 cut(s) 295, 1078, 1155, 1525
BmcAI AGTACT 1 cut(s) 1060
Bme1390I CCNGG 5 cut(s) 282, 473, 594, 781, 969
Bme18I GGWCC 1 cut(s) 1112
BmgBI CACGTC 1 cut(s) 195
BmgT120I GGNCC 3 cut(s) 110, 1112, 1669
BmrFI CCNGG 5 cut(s) 282, 473, 594, 781, 969
BmrI ACTGGG 1 cut(s) 524
BmsI GCATC 5 cut(s) 280, 322, 394, 1057, 1360
BmuI ACTGGG 1 cut(s) 524
BpiI GAAGAC 3 cut(s) 57, 573, 574
BpmI CTGGAG 2 cut(s) 129, 1455
Bpu10I CCTNAGC 1 cut(s) 1677
Bpu14I TTCGAA 1 cut(s) 171
BpuEI CTTGAG 2 cut(s) 99, 1649
BsaAI YACGTR 2 cut(s) 1419, 1511
BsaHI GRCGYC 1 cut(s) 1466
BsaI GGTCTC 3 cut(s) 60, 335, 866
BsaJI CCNNGG 4 cut(s) 45, 280, 353, 967
BsaWI WCCGGW 2 cut(s) 1143, 1559
BsaXI ACNNNNNCTCC 2 cut(s) 714, 744
Bsc4I CCNNNNNNNGG 3 cut(s) 895, 1121, 1678
Bse118I RCCGGY 1 cut(s) 1559
Bse1I ACTGG 3 cut(s) 530, 567, 1092
Bse3DI GCAATG 1 cut(s) 82
BseAI TCCGGA 1 cut(s) 1143
BseBI CCWGG 5 cut(s) 282, 473, 594, 781, 969
BseDI CCNNGG 4 cut(s) 45, 280, 353, 967
BseGI GGATG 3 cut(s) 494, 1235, 1375
BseLI CCNNNNNNNGG 3 cut(s) 895, 1121, 1678
BseMI GCAATG 1 cut(s) 82
BseMII CTCAG 6 cut(s) 385, 792, 1347, 1365, 1412, 1668
BseNI ACTGG 3 cut(s) 530, 567, 1092
BseXI GCAGC 4 cut(s) 305, 1063, 1140, 1510
BsgI GTGCAG 2 cut(s) 1331, 1509
Bsh1236I CGCG 1 cut(s) 1494
BshFI GGCC 6 cut(s) 112, 285, 358, 888, 1256, 1670
BshTI ACCGGT 1 cut(s) 1559
BsiHKAI GWGCWC 2 cut(s) 1516, 1665
BsiSI CCGG 2 cut(s) 1144, 1560
BslFI GGGAC 1 cut(s) 263
BslI CCNNNNNNNGG 3 cut(s) 895, 1121, 1678
BsmAI GTCTC 3 cut(s) 60, 335, 866
BsmFI GGGAC 1 cut(s) 263
BsnI GGCC 6 cut(s) 112, 285, 358, 888, 1256, 1670
Bso31I GGTCTC 3 cut(s) 60, 335, 866
Bsp119I TTCGAA 1 cut(s) 171
Bsp1286I GDGCHC 2 cut(s) 1516, 1665
Bsp13I TCCGGA 1 cut(s) 1143
Bsp1407I TGTACA 1 cut(s) 957
Bsp143I GATC 6 cut(s) 448, 490, 517, 626, 977, 1029
BspACI CCGC 2 cut(s) 1492, 1658
BspANI GGCC 6 cut(s) 112, 285, 358, 888, 1256, 1670
BspCNI CTCAG 6 cut(s) 384, 793, 1346, 1366, 1411, 1669
BspEI TCCGGA 1 cut(s) 1143
BspFNI CGCG 1 cut(s) 1494
BspHI TCATGA 1 cut(s) 1051
BspMI ACCTGC 1 cut(s) 116
BspPI GGATC 4 cut(s) 443, 485, 634, 1024
BspT104I TTCGAA 1 cut(s) 171
BspTI CTTAAG 1 cut(s) 323
BspTNI GGTCTC 3 cut(s) 60, 335, 866
BsrDI GCAATG 1 cut(s) 82
BsrFI RCCGGY 1 cut(s) 1559
BsrGI TGTACA 1 cut(s) 957
BsrI ACTGG 3 cut(s) 530, 567, 1092
BssAI RCCGGY 1 cut(s) 1559
BssECI CCNNGG 4 cut(s) 45, 280, 353, 967
BssMI GATC 6 cut(s) 448, 490, 517, 626, 977, 1029
BssNI GRCGYC 1 cut(s) 1466
BssSI CACGAG 1 cut(s) 935
BssT1I CCWWGG 1 cut(s) 353
Bst2BI CACGAG 1 cut(s) 935
Bst2UI CCWGG 5 cut(s) 282, 473, 594, 781, 969
Bst4CI ACNGT 4 cut(s) 17, 877, 1058, 1582
Bst6I CTCTTC 5 cut(s) 168, 435, 615, 672, 1700
BstACI GRCGYC 1 cut(s) 1466
BstAFI CTTAAG 1 cut(s) 323
BstAPI GCANNNNNTGC 2 cut(s) 1076, 1370
BstAUI TGTACA 1 cut(s) 957
BstBAI YACGTR 2 cut(s) 1419, 1511
BstBI TTCGAA 1 cut(s) 171
BstC8I GCNNGC 2 cut(s) 204, 1412
BstDEI CTNAG 8 cut(s) 371, 801, 995, 1333, 1374, 1398, 1654, 1677
BstF5I GGATG 3 cut(s) 494, 1235, 1375
BstFNI CGCG 1 cut(s) 1494
BstH2I RGCGCY 2 cut(s) 1094, 1222
BstHHI GCGC 3 cut(s) 409, 1093, 1221
BstKTI GATC 6 cut(s) 451, 493, 520, 629, 980, 1032
BstMAI GTCTC 3 cut(s) 60, 335, 866
BstMBI GATC 6 cut(s) 448, 490, 517, 626, 977, 1029
BstMWI GCNNNNNNNGC 5 cut(s) 1076, 1370, 1520, 1609, 1682
BstNI CCWGG 5 cut(s) 282, 473, 594, 781, 969
BstNSI RCATGY 1 cut(s) 420
BstSCI CCNGG 5 cut(s) 280, 471, 592, 779, 967
BstSFI CTRYAG 1 cut(s) 1581
BstUI CGCG 1 cut(s) 1494
BstV1I GCAGC 4 cut(s) 305, 1063, 1140, 1510
BstV2I GAAGAC 3 cut(s) 57, 573, 574
BstX2I RGATCY 2 cut(s) 517, 1029
BstYI RGATCY 2 cut(s) 517, 1029
BsuRI GGCC 6 cut(s) 112, 285, 358, 888, 1256, 1670
BtrI CACGTC 1 cut(s) 195
BtsCI GGATG 3 cut(s) 494, 1235, 1375
BveI ACCTGC 1 cut(s) 116
Cac8I GCNNGC 2 cut(s) 204, 1412
CciI TCATGA 1 cut(s) 1051
CfoI GCGC 3 cut(s) 409, 1093, 1221
Cfr10I RCCGGY 1 cut(s) 1559
Cfr13I GGNCC 3 cut(s) 110, 1112, 1669
CseI GACGC 2 cut(s) 712, 1333
CsiI ACCWGGT 2 cut(s) 592, 779
Csp6I GTAC 4 cut(s) 179, 958, 1059, 1557
CspAI ACCGGT 1 cut(s) 1559
CviAII CATG 8 cut(s) 217, 386, 417, 1052, 1109, 1241, 1543, 1565
CviQI GTAC 4 cut(s) 179, 958, 1059, 1557
DdeI CTNAG 8 cut(s) 371, 801, 995, 1333, 1374, 1398, 1654, 1677
DpnI GATC 6 cut(s) 450, 492, 519, 628, 979, 1031
DpnII GATC 6 cut(s) 448, 490, 517, 626, 977, 1029
DraI TTTAAA 2 cut(s) 1098, 1353
DraIII CACNNNGTG 1 cut(s) 1629
EaeI YGGCCR 1 cut(s) 886
Eam1104I CTCTTC 5 cut(s) 168, 435, 615, 672, 1700
EarI CTCTTC 5 cut(s) 168, 435, 615, 672, 1700
Ecl136II GAGCTC 1 cut(s) 1663
Eco130I CCWWGG 1 cut(s) 353
Eco147I AGGCCT 2 cut(s) 285, 358
Eco24I GRGCYC 1 cut(s) 1665
Eco31I GGTCTC 3 cut(s) 60, 335, 866
Eco32I GATATC 1 cut(s) 1016
Eco47I GGWCC 1 cut(s) 1112
Eco47III AGCGCT 1 cut(s) 1220
Eco53kI GAGCTC 1 cut(s) 1663
Eco57I CTGAAG 1 cut(s) 1083
EcoICRI GAGCTC 1 cut(s) 1663
EcoRII CCWGG 5 cut(s) 280, 471, 592, 779, 967
EcoRV GATATC 1 cut(s) 1016
EcoT14I CCWWGG 1 cut(s) 353
EcoT22I ATGCAT 1 cut(s) 1246
EcoT38I GRGCYC 1 cut(s) 1665
ErhI CCWWGG 1 cut(s) 353
FaeI CATG 8 cut(s) 220, 389, 420, 1055, 1112, 1244, 1546, 1568
FaqI GGGAC 1 cut(s) 263
FatI CATG 8 cut(s) 216, 385, 416, 1051, 1108, 1240, 1542, 1564
Fnu4HI GCNGC 4 cut(s) 294, 1077, 1154, 1524
FokI GGATG 3 cut(s) 481, 1222, 1382
FriOI GRGCYC 1 cut(s) 1665
Fsp4HI GCNGC 4 cut(s) 294, 1077, 1154, 1524
FspBI CTAG 5 cut(s) 188, 287, 456, 881, 1503
GlaI GCGC 3 cut(s) 408, 1092, 1220
GluI GCNGC 4 cut(s) 294, 1077, 1154, 1524
GsuI CTGGAG 2 cut(s) 129, 1455
HaeII RGCGCY 2 cut(s) 1094, 1222
HaeIII GGCC 6 cut(s) 112, 285, 358, 888, 1256, 1670
HapII CCGG 2 cut(s) 1144, 1560
HgaI GACGC 2 cut(s) 712, 1333
HhaI GCGC 3 cut(s) 409, 1093, 1221
Hin1I GRCGYC 1 cut(s) 1466
Hin1II CATG 8 cut(s) 220, 389, 420, 1055, 1112, 1244, 1546, 1568
Hin6I GCGC 3 cut(s) 407, 1091, 1219
HinP1I GCGC 3 cut(s) 407, 1091, 1219
HincII GTYRAC 1 cut(s) 974
HindII GTYRAC 1 cut(s) 974
HindIII AAGCTT 1 cut(s) 1408
HinfI GANTC 6 cut(s) 56, 142, 213, 792, 1232, 1357
HpaII CCGG 2 cut(s) 1144, 1560
HphI GGTGA 1 cut(s) 829
Hpy166II GTNNAC 3 cut(s) 865, 974, 1498
Hpy188I TCNGA 9 cut(s) 165, 396, 513, 1231, 1261, 1336, 1401, 1471, 1609
Hpy8I GTNNAC 3 cut(s) 865, 974, 1498
Hpy99I CGWCG 2 cut(s) 226, 706
HpyAV CCTTC 2 cut(s) 15, 630
HpyCH4III ACNGT 4 cut(s) 17, 877, 1058, 1582
HpyCH4IV ACGT 5 cut(s) 102, 194, 1418, 1466, 1510
HpyCH4V TGCA 6 cut(s) 293, 420, 1070, 1244, 1312, 1526
HpyF10VI GCNNNNNNNGC 5 cut(s) 1076, 1370, 1520, 1609, 1682
HpyF3I CTNAG 8 cut(s) 371, 801, 995, 1333, 1374, 1398, 1654, 1677
HpySE526I ACGT 5 cut(s) 102, 194, 1418, 1466, 1510
Hsp92I GRCGYC 1 cut(s) 1466
Hsp92II CATG 8 cut(s) 220, 389, 420, 1055, 1112, 1244, 1546, 1568
HspAI GCGC 3 cut(s) 407, 1091, 1219
Kpn2I TCCGGA 1 cut(s) 1143
Kzo9I GATC 6 cut(s) 448, 490, 517, 626, 977, 1029
LmnI GCTCC 4 cut(s) 414, 622, 1609, 1660
Lsp1109I GCAGC 4 cut(s) 305, 1063, 1140, 1510
LweI GCATC 5 cut(s) 280, 322, 394, 1057, 1360
MabI ACCWGGT 2 cut(s) 592, 779
MaeI CTAG 5 cut(s) 188, 287, 456, 881, 1503
MaeII ACGT 5 cut(s) 102, 194, 1418, 1466, 1510
MaeIII GTNAC 7 cut(s) 190, 381, 532, 670, 709, 817, 1204
MalI GATC 6 cut(s) 450, 492, 519, 628, 979, 1031
MboI GATC 6 cut(s) 448, 490, 517, 626, 977, 1029
MflI RGATCY 2 cut(s) 517, 1029
MhlI GDGCHC 2 cut(s) 1516, 1665
MlsI TGGCCA 1 cut(s) 888
MluCI AATT 9 cut(s) 91, 134, 166, 740, 921, 1328, 1337, 1379, 1482
MluNI TGGCCA 1 cut(s) 888
MlyI GAGTC 2 cut(s) 50, 136
Mox20I TGGCCA 1 cut(s) 888
Mph1103I ATGCAT 1 cut(s) 1246
MroI TCCGGA 1 cut(s) 1143
MscI TGGCCA 1 cut(s) 888
MseI TTAA 7 cut(s) 138, 324, 747, 854, 1097, 1352, 1592
Msp20I TGGCCA 1 cut(s) 888
MspCI CTTAAG 1 cut(s) 323
MspI CCGG 2 cut(s) 1144, 1560
MspR9I CCNGG 5 cut(s) 282, 473, 594, 781, 969
MvaI CCWGG 5 cut(s) 282, 473, 594, 781, 969
MvnI CGCG 1 cut(s) 1494
MwoI GCNNNNNNNGC 5 cut(s) 1076, 1370, 1520, 1609, 1682
NdeII GATC 6 cut(s) 448, 490, 517, 626, 977, 1029
NlaIII CATG 8 cut(s) 220, 389, 420, 1055, 1112, 1244, 1546, 1568
NmuCI GTSAC 5 cut(s) 190, 381, 670, 709, 817
NsiI ATGCAT 1 cut(s) 1246
NspI RCATGY 1 cut(s) 420
NspV TTCGAA 1 cut(s) 171
PagI TCATGA 1 cut(s) 1051
PaqCI CACCTGC 1 cut(s) 116
PceI AGGCCT 2 cut(s) 285, 358
PfeI GAWTC 4 cut(s) 213, 792, 1232, 1357
PflMI CCANNNNNTGG 1 cut(s) 895
PinAI ACCGGT 1 cut(s) 1559
PkrI GCNGC 4 cut(s) 295, 1078, 1155, 1525
PleI GAGTC 2 cut(s) 50, 136
PpsI GAGTC 2 cut(s) 50, 136
Ppu21I YACGTR 2 cut(s) 1419, 1511
PsiI TTATAA 1 cut(s) 774
Psp124BI GAGCTC 1 cut(s) 1665
Psp6I CCWGG 5 cut(s) 280, 471, 592, 779, 967
PspGI CCWGG 5 cut(s) 280, 471, 592, 779, 967
PspPI GGNCC 3 cut(s) 110, 1112, 1669
PsuI RGATCY 2 cut(s) 517, 1029
RsaI GTAC 4 cut(s) 180, 959, 1060, 1558
RsaNI GTAC 4 cut(s) 179, 958, 1059, 1557
SacI GAGCTC 1 cut(s) 1665
SaqAI TTAA 7 cut(s) 138, 324, 747, 854, 1097, 1352, 1592
SatI GCNGC 4 cut(s) 294, 1077, 1154, 1524
Sau3AI GATC 6 cut(s) 448, 490, 517, 626, 977, 1029
Sau96I GGNCC 3 cut(s) 110, 1112, 1669
ScaI AGTACT 1 cut(s) 1060
SchI GAGTC 2 cut(s) 50, 136
ScrFI CCNGG 5 cut(s) 282, 473, 594, 781, 969
SduI GDGCHC 2 cut(s) 1516, 1665
SexAI ACCWGGT 2 cut(s) 592, 779
SfaNI GCATC 5 cut(s) 280, 322, 394, 1057, 1360
SfcI CTRYAG 1 cut(s) 1581
SfuI TTCGAA 1 cut(s) 171
SinI GGWCC 1 cut(s) 1112
SmlI CTYRAG 3 cut(s) 114, 323, 1664
SmoI CTYRAG 3 cut(s) 114, 323, 1664
SpeI ACTAGT 1 cut(s) 187
Sse9I AATT 9 cut(s) 91, 134, 166, 740, 921, 1328, 1337, 1379, 1482
SseBI AGGCCT 2 cut(s) 285, 358
SsiI CCGC 2 cut(s) 1492, 1658
SspMI CTAG 5 cut(s) 188, 287, 456, 881, 1503
SstI GAGCTC 1 cut(s) 1665
StuI AGGCCT 2 cut(s) 285, 358
StyD4I CCNGG 5 cut(s) 280, 471, 592, 779, 967
StyI CCWWGG 1 cut(s) 353
TaaI ACNGT 4 cut(s) 17, 877, 1058, 1582
TaiI ACGT 5 cut(s) 105, 197, 1421, 1469, 1513
TaqI TCGA 4 cut(s) 89, 171, 316, 333
TasI AATT 9 cut(s) 91, 134, 166, 740, 921, 1328, 1337, 1379, 1482
TatI WGTACW 2 cut(s) 957, 1058
TfiI GAWTC 4 cut(s) 213, 792, 1232, 1357
Tru1I TTAA 7 cut(s) 138, 324, 747, 854, 1097, 1352, 1592
Tru9I TTAA 7 cut(s) 138, 324, 747, 854, 1097, 1352, 1592
TseFI GTSAC 5 cut(s) 190, 381, 670, 709, 817
TseI GCWGC 4 cut(s) 293, 1076, 1153, 1523
Tsp45I GTSAC 5 cut(s) 190, 381, 670, 709, 817
TspDTI ATGAA 4 cut(s) 205, 402, 654, 882
Van91I CCANNNNNTGG 1 cut(s) 895
Vha464I CTTAAG 1 cut(s) 323
VpaK11BI GGWCC 1 cut(s) 1112
XapI RAATTY 4 cut(s) 91, 134, 166, 1482
XceI RCATGY 1 cut(s) 420
XcmI CCANNNNNNNNNTGG 1 cut(s) 1430
XspI CTAG 5 cut(s) 188, 287, 456, 881, 1503
ZraI GACGTC 1 cut(s) 1467
ZrmI AGTACT 1 cut(s) 1060
Zsp2I ATGCAT 1 cut(s) 1246
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.