Rroxscaffold_7G00191200

Small RNA degrading nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0003406
Physical Location & Seq
Reverse (-)
31734851 .. 31743573
8723 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00191200.1

Sequence Viewer

Length: 1896 bp
ATGCCTTCGTCCCACAGTAAATGCGAAGAAGAAGTGTTAGCTACCGAGGAAGACGACTCAAACAGAGACGAAACCAATGCCAAAAGCTCGAATTTCTATGACGTTTACGGGCCTCAAGCTAAAGCAGGTGTGGAATTTAAGACTCCAGAAGCTACTTCAACGCTGAATTTCGAAGAAGTACAACAACTAGTGACGTGGGTGCTTGCTGATGGATTCATGCCGTCGTGGGTTTTTGTAAAGAACAAGCCCCTCATTCCCAAAGTGGTTATGCTTTATGTTCCAGGCCTAGATGCAGCTCTCTACTTATCACAGCATCGACTACTTAAGGGTCTCGAAAAATGTTGTGGAAAACCAAGGCCGGTTTTAGCACTCAGCTATATGTCACATGAAAATCAGACGATTGATGCGCTCCTTACATGCAAACTGAAAAGAAAGAGGGAAGAGAGTGATCCAGCTAGGACAAAATCTAACCTGGAAACTCAACAAGATGATCCATCCTCTGTGGAACTTCTGAAAGATCTACCATTCCCAGTTACATTTTACACTCTTACGACAAAACAACTGGAAGACAATGGATATGTCTTCAATCAACCAGGTTTTCTTTCTACTCTTCCTGCTCCTTCTGGATCATCTCCTTATGAAATGTTAGCACTTGATTGTGAGATGTGTGTCACCGAAGAGGGTTTTGAGCTAACGAGAGCGACGCTTGTGACTATAAAAGGAGAGGGAAGGAGAAAACATTATGGGGATGCTAGAGCTTCAACAACTTTTCTTATTCTCAATTCAGCATACCATCCTGCTGATGACACATCTGAATCTAGAAATCTCGGGTCTAATAGGGCAACACTTGATATGAAGGAAAGGGTTGTGCTTGATAAATTGGTTAAACCAACAAATGCTATCAAAGATTATAACACCAGGTTTAGTGGAATCACACCTGAGATGTTAGATGGGGTGACTACAAGCCTCAAAGATATTCAGGTTCATTACTTGGGTCTTGCTCTGCATAATGTACACATGCATGCAGAGGACTTCTTAAAGCTGGTTTACAAAGAGACCGTCTTAGTTGGCCATTCGTTGGAGAATGACTTATTAGCATTGAAAATTGCTCACAATCTCGTGATTGATACAGCAGTATTGTACAAGCACCCCGGTCAACGATCTTACAAATCTGCTCTTAGAGTTCTTGCCAAGAGATATCTTTCTAGAGAGATCCAGCAGTCAGGGAATGGTCATGACAGTACTGAAGATGCAAGAGCTGCTATGGAACTGGCGCTTTTAAAGATTAGACATGGACCTGACTTTGGCACAGCACCATCAGTTTTCCGGAAAAAGCTGCTAAAAGTTTTGAGTGATAATGGAAAGACCACCTCTATCATTGATGATGTTACCATTGTGAAGCGCTATGCTTCTGAATCATCCCATGCATTTCCTGTTGCCTCTGATGAGGAAGCTCTATCTAAAGCTGTAAAGGAGGCAAGGAACGAGAAAGTGCAGTTTGTCTGGACGCAATTCTCAGAATTATATCGCTTTTTAAAGAACCAAGCAGAGGATGCTGAGAAATTAGATGTAAGACTTGCTCAGATGATAAGCTTGCTTACATGCCTTAAGAAGTCTGGAGATAAAAAGGGTATTAGATGGAGTTTGACGTCTGAACTAAAGGAAATTCTTATCCGCATGGACACTAGAATACGTACTCTATATGCTGCACTACCCACTAATGCCATGCTTATCATTTGTACCGGTCATGGAGATATAGCAACTGTAGATAGATTAAACAAAATGCTTTCGGAGCGTGAGGAAACCACGATGTGTCGGGATAGCATTGTAAAGGTCTTAGCGGAGCTCAAGGCCCAAGCTGAGGTAGCTTTATGTTTTGTGGGTGTGAAGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
RNase_T

Protein Analysis

631

Amino Acids

70.62

Weight (kDa)

6.5

Isoelectric Point (pI)

35.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_T PF00929 286 - 424 3.2e-08 Exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 912
AarI CACCTGC 1 cut(s) 116
AatII GACGTC 1 cut(s) 1652
Acc36I ACCTGC 1 cut(s) 116
AccB7I CCANNNNNTGG 1 cut(s) 1078
AccIII TCCGGA 1 cut(s) 1326
AciI CCGC 2 cut(s) 1675, 1841
AclWI GGATC 4 cut(s) 443, 485, 634, 1207
AcoI YGGCCR 1 cut(s) 1069
AcsI RAATTY 4 cut(s) 91, 134, 166, 1665
AcuI CTGAAG 1 cut(s) 1266
AcyI GRCGYC 1 cut(s) 1649
AdeI CACNNNGTG 1 cut(s) 1812
AfaI GTAC 6 cut(s) 180, 1014, 1142, 1243, 1696, 1741
AfeI AGCGCT 1 cut(s) 1403
AfiI CCNNNNNNNGG 4 cut(s) 1078, 1304, 1549, 1861
AflII CTTAAG 2 cut(s) 323, 1607
AgeI ACCGGT 1 cut(s) 1742
AgsI TTSAA 4 cut(s) 159, 586, 762, 1102
AhlI ACTAGT 1 cut(s) 187
AjiI CACGTC 1 cut(s) 195
AjnI CCWGG 4 cut(s) 280, 471, 592, 917
Alw21I GWGCWC 1 cut(s) 1848
Alw26I GTCTC 3 cut(s) 60, 335, 1049
AlwI GGATC 4 cut(s) 443, 485, 634, 1207
Ama87I CYCGRG 1 cut(s) 827
Aor13HI TCCGGA 1 cut(s) 1326
Aor51HI AGCGCT 1 cut(s) 1403
AoxI GGCC 5 cut(s) 110, 283, 356, 1069, 1851
ApeKI GCWGC 4 cut(s) 293, 1259, 1336, 1706
ApoI RAATTY 4 cut(s) 91, 134, 166, 1665
ArsI GACNNNNNNTTYG 2 cut(s) 1044, 1076
AsiGI ACCGGT 1 cut(s) 1742
AspLEI GCGC 3 cut(s) 409, 1276, 1404
AspS9I GGNCC 3 cut(s) 110, 1295, 1852
AsuC2I CCSGG 1 cut(s) 1152
AsuHPI GGTGA 2 cut(s) 664, 967
AsuII TTCGAA 1 cut(s) 171
AvaI CYCGRG 1 cut(s) 827
AvaII GGWCC 1 cut(s) 1295
BaeI ACNNNNGTAYC 2 cut(s) 1119, 1152
BalI TGGCCA 1 cut(s) 1071
BanII GRGCYC 1 cut(s) 1848
BauI CACGAG 1 cut(s) 1118
BbsI GAAGAC 3 cut(s) 57, 573, 574
Bbv12I GWGCWC 1 cut(s) 1848
BbvCI CCTCAGC 1 cut(s) 1860
BbvI GCAGC 4 cut(s) 305, 1246, 1323, 1693
BccI CCATC 6 cut(s) 203, 502, 801, 944, 1324, 1632
BceAI ACGGC 1 cut(s) 205
BcgI CGANNNNNNTGC 2 cut(s) 59, 93
BciT130I CCWGG 4 cut(s) 282, 473, 594, 919
BcnI CCSGG 1 cut(s) 1152
BcoDI GTCTC 3 cut(s) 60, 335, 1049
BcuI ACTAGT 1 cut(s) 187
BfaI CTAG 7 cut(s) 188, 287, 456, 753, 819, 1206, 1686
BfmI CTRYAG 1 cut(s) 1764
BfoI RGCGCY 2 cut(s) 1277, 1405
BfrI CTTAAG 2 cut(s) 323, 1607
BfuAI ACCTGC 1 cut(s) 116
BglII AGATCT 1 cut(s) 517
BisI GCNGC 4 cut(s) 294, 1260, 1337, 1707
BlsI GCNGC 4 cut(s) 295, 1261, 1338, 1708
BmcAI AGTACT 1 cut(s) 1243
Bme1390I CCNGG 5 cut(s) 282, 473, 594, 919, 1152
Bme18I GGWCC 1 cut(s) 1295
BmeT110I CYCGRG 1 cut(s) 827
BmgBI CACGTC 1 cut(s) 195
BmgT120I GGNCC 3 cut(s) 110, 1295, 1852
BmrFI CCNGG 5 cut(s) 282, 473, 594, 919, 1152
BmrI ACTGGG 1 cut(s) 524
BmsI GCATC 6 cut(s) 280, 322, 394, 739, 1240, 1543
BmuI ACTGGG 1 cut(s) 524
BpiI GAAGAC 3 cut(s) 57, 573, 574
BpmI CTGGAG 2 cut(s) 129, 1638
Bpu10I CCTNAGC 1 cut(s) 1860
Bpu14I TTCGAA 1 cut(s) 171
BpuEI CTTGAG 2 cut(s) 99, 1832
BpuMI CCSGG 1 cut(s) 1152
BsaAI YACGTR 1 cut(s) 1694
BsaHI GRCGYC 1 cut(s) 1649
BsaI GGTCTC 2 cut(s) 335, 1049
BsaJI CCNNGG 3 cut(s) 45, 353, 1150
BsaWI WCCGGW 2 cut(s) 1326, 1742
Bsc4I CCNNNNNNNGG 4 cut(s) 1078, 1304, 1549, 1861
Bse118I RCCGGY 2 cut(s) 358, 1742
Bse1I ACTGG 3 cut(s) 530, 567, 1275
BseAI TCCGGA 1 cut(s) 1326
BseBI CCWGG 4 cut(s) 282, 473, 594, 919
BseDI CCNNGG 3 cut(s) 45, 353, 1150
BseGI GGATG 5 cut(s) 494, 754, 793, 1418, 1558
BseLI CCNNNNNNNGG 4 cut(s) 1078, 1304, 1549, 1861
BseMII CTCAG 6 cut(s) 385, 930, 1530, 1548, 1595, 1851
BseNI ACTGG 3 cut(s) 530, 567, 1275
BseXI GCAGC 4 cut(s) 305, 1246, 1323, 1693
BsgI GTGCAG 2 cut(s) 1514, 1692
BshFI GGCC 5 cut(s) 112, 285, 358, 1071, 1853
BshTI ACCGGT 1 cut(s) 1742
BsiHKAI GWGCWC 1 cut(s) 1848
BsiHKCI CYCGRG 1 cut(s) 827
BsiSI CCGG 4 cut(s) 359, 1152, 1327, 1743
BslI CCNNNNNNNGG 4 cut(s) 1078, 1304, 1549, 1861
BsmAI GTCTC 3 cut(s) 60, 335, 1049
BsmBI CGTCTC 1 cut(s) 60
BsnI GGCC 5 cut(s) 112, 285, 358, 1071, 1853
Bso31I GGTCTC 2 cut(s) 335, 1049
BsoBI CYCGRG 1 cut(s) 827
Bsp119I TTCGAA 1 cut(s) 171
Bsp1286I GDGCHC 1 cut(s) 1848
Bsp13I TCCGGA 1 cut(s) 1326
Bsp1407I TGTACA 2 cut(s) 1012, 1140
Bsp143I GATC 6 cut(s) 448, 490, 517, 626, 1160, 1212
BspACI CCGC 2 cut(s) 1675, 1841
BspANI GGCC 5 cut(s) 112, 285, 358, 1071, 1853
BspCNI CTCAG 6 cut(s) 384, 931, 1529, 1549, 1594, 1852
BspEI TCCGGA 1 cut(s) 1326
BspHI TCATGA 1 cut(s) 1234
BspMI ACCTGC 1 cut(s) 116
BspPI GGATC 4 cut(s) 443, 485, 634, 1207
BspT104I TTCGAA 1 cut(s) 171
BspTI CTTAAG 2 cut(s) 323, 1607
BspTNI GGTCTC 2 cut(s) 335, 1049
BsrFI RCCGGY 2 cut(s) 358, 1742
BsrGI TGTACA 2 cut(s) 1012, 1140
BsrI ACTGG 3 cut(s) 530, 567, 1275
BssAI RCCGGY 2 cut(s) 358, 1742
BssECI CCNNGG 3 cut(s) 45, 353, 1150
BssMI GATC 6 cut(s) 448, 490, 517, 626, 1160, 1212
BssNI GRCGYC 1 cut(s) 1649
BssSI CACGAG 1 cut(s) 1118
BssT1I CCWWGG 1 cut(s) 353
Bst2BI CACGAG 1 cut(s) 1118
Bst2UI CCWGG 4 cut(s) 282, 473, 594, 919
Bst4CI ACNGT 4 cut(s) 17, 1060, 1241, 1765
Bst6I CTCTTC 4 cut(s) 435, 615, 672, 1883
BstACI GRCGYC 1 cut(s) 1649
BstAFI CTTAAG 2 cut(s) 323, 1607
BstAPI GCANNNNNTGC 2 cut(s) 1259, 1553
BstAUI TGTACA 2 cut(s) 1012, 1140
BstBAI YACGTR 1 cut(s) 1694
BstBI TTCGAA 1 cut(s) 171
BstC8I GCNNGC 3 cut(s) 204, 1023, 1595
BstDEI CTNAG 9 cut(s) 371, 939, 1063, 1178, 1516, 1557, 1581, 1837, 1860
BstF5I GGATG 5 cut(s) 494, 754, 793, 1418, 1558
BstH2I RGCGCY 2 cut(s) 1277, 1405
BstHHI GCGC 3 cut(s) 409, 1276, 1404
BstKTI GATC 6 cut(s) 451, 493, 520, 629, 1163, 1215
BstMAI GTCTC 3 cut(s) 60, 335, 1049
BstMBI GATC 6 cut(s) 448, 490, 517, 626, 1160, 1212
BstMWI GCNNNNNNNGC 4 cut(s) 1259, 1553, 1792, 1865
BstNI CCWGG 4 cut(s) 282, 473, 594, 919
BstNSI RCATGY 4 cut(s) 420, 1021, 1025, 1605
BstSCI CCNGG 5 cut(s) 280, 471, 592, 917, 1150
BstSFI CTRYAG 1 cut(s) 1764
BstSNI TACGTA 1 cut(s) 1694
BstV1I GCAGC 4 cut(s) 305, 1246, 1323, 1693
BstV2I GAAGAC 3 cut(s) 57, 573, 574
BstX2I RGATCY 2 cut(s) 517, 1212
BstYI RGATCY 2 cut(s) 517, 1212
BsuRI GGCC 5 cut(s) 112, 285, 358, 1071, 1853
BtrI CACGTC 1 cut(s) 195
BtsCI GGATG 5 cut(s) 494, 754, 793, 1418, 1558
BveI ACCTGC 1 cut(s) 116
Cac8I GCNNGC 3 cut(s) 204, 1023, 1595
CciI TCATGA 1 cut(s) 1234
CfoI GCGC 3 cut(s) 409, 1276, 1404
Cfr10I RCCGGY 2 cut(s) 358, 1742
Cfr13I GGNCC 3 cut(s) 110, 1295, 1852
CseI GACGC 2 cut(s) 712, 1516
CsiI ACCWGGT 2 cut(s) 592, 917
Csp6I GTAC 6 cut(s) 179, 1013, 1141, 1242, 1695, 1740
CspAI ACCGGT 1 cut(s) 1742
CviQI GTAC 6 cut(s) 179, 1013, 1141, 1242, 1695, 1740
DdeI CTNAG 9 cut(s) 371, 939, 1063, 1178, 1516, 1557, 1581, 1837, 1860
DpnI GATC 6 cut(s) 450, 492, 519, 628, 1162, 1214
DpnII GATC 6 cut(s) 448, 490, 517, 626, 1160, 1212
DraI TTTAAA 2 cut(s) 1281, 1536
DraIII CACNNNGTG 1 cut(s) 1812
EaeI YGGCCR 1 cut(s) 1069
Eam1104I CTCTTC 4 cut(s) 435, 615, 672, 1883
EarI CTCTTC 4 cut(s) 435, 615, 672, 1883
Ecl136II GAGCTC 1 cut(s) 1846
Eco105I TACGTA 1 cut(s) 1694
Eco130I CCWWGG 1 cut(s) 353
Eco147I AGGCCT 1 cut(s) 285
Eco24I GRGCYC 1 cut(s) 1848
Eco31I GGTCTC 2 cut(s) 335, 1049
Eco32I GATATC 1 cut(s) 1199
Eco47I GGWCC 1 cut(s) 1295
Eco47III AGCGCT 1 cut(s) 1403
Eco53kI GAGCTC 1 cut(s) 1846
Eco57I CTGAAG 1 cut(s) 1266
Eco88I CYCGRG 1 cut(s) 827
EcoICRI GAGCTC 1 cut(s) 1846
EcoRII CCWGG 4 cut(s) 280, 471, 592, 917
EcoRV GATATC 1 cut(s) 1199
EcoT14I CCWWGG 1 cut(s) 353
EcoT22I ATGCAT 2 cut(s) 1023, 1429
EcoT38I GRGCYC 1 cut(s) 1848
ErhI CCWWGG 1 cut(s) 353
Esp3I CGTCTC 1 cut(s) 60
Fnu4HI GCNGC 4 cut(s) 294, 1260, 1337, 1707
FokI GGATG 5 cut(s) 481, 761, 780, 1405, 1565
FriOI GRGCYC 1 cut(s) 1848
Fsp4HI GCNGC 4 cut(s) 294, 1260, 1337, 1707
FspBI CTAG 7 cut(s) 188, 287, 456, 753, 819, 1206, 1686
GlaI GCGC 3 cut(s) 408, 1275, 1403
GluI GCNGC 4 cut(s) 294, 1260, 1337, 1707
GsuI CTGGAG 2 cut(s) 129, 1638
HaeII RGCGCY 2 cut(s) 1277, 1405
HaeIII GGCC 5 cut(s) 112, 285, 358, 1071, 1853
HapII CCGG 4 cut(s) 359, 1152, 1327, 1743
HgaI GACGC 2 cut(s) 712, 1516
HhaI GCGC 3 cut(s) 409, 1276, 1404
Hin1I GRCGYC 1 cut(s) 1649
Hin6I GCGC 3 cut(s) 407, 1274, 1402
HinP1I GCGC 3 cut(s) 407, 1274, 1402
HincII GTYRAC 1 cut(s) 1157
HindII GTYRAC 1 cut(s) 1157
HindIII AAGCTT 1 cut(s) 1591
HinfI GANTC 6 cut(s) 56, 142, 213, 815, 930, 1415
HpaII CCGG 4 cut(s) 359, 1152, 1327, 1743
HphI GGTGA 2 cut(s) 664, 967
Hpy166II GTNNAC 4 cut(s) 106, 1015, 1048, 1157
Hpy188I TCNGA 9 cut(s) 396, 513, 814, 1414, 1444, 1519, 1584, 1654, 1792
Hpy8I GTNNAC 4 cut(s) 106, 1015, 1048, 1157
Hpy99I CGWCG 2 cut(s) 226, 706
HpyAV CCTTC 4 cut(s) 15, 630, 723, 850
HpyCH4III ACNGT 4 cut(s) 17, 1060, 1241, 1765
HpyCH4IV ACGT 4 cut(s) 102, 194, 1649, 1693
HpyCH4V TGCA 9 cut(s) 293, 420, 1006, 1021, 1025, 1253, 1427, 1495, 1709
HpyF10VI GCNNNNNNNGC 4 cut(s) 1259, 1553, 1792, 1865
HpyF3I CTNAG 9 cut(s) 371, 939, 1063, 1178, 1516, 1557, 1581, 1837, 1860
HpySE526I ACGT 4 cut(s) 102, 194, 1649, 1693
Hsp92I GRCGYC 1 cut(s) 1649
HspAI GCGC 3 cut(s) 407, 1274, 1402
Kpn2I TCCGGA 1 cut(s) 1326
Kzo9I GATC 6 cut(s) 448, 490, 517, 626, 1160, 1212
LmnI GCTCC 4 cut(s) 414, 622, 1792, 1843
Lsp1109I GCAGC 4 cut(s) 305, 1246, 1323, 1693
LweI GCATC 6 cut(s) 280, 322, 394, 739, 1240, 1543
MabI ACCWGGT 2 cut(s) 592, 917
MaeI CTAG 7 cut(s) 188, 287, 456, 753, 819, 1206, 1686
MaeII ACGT 4 cut(s) 102, 194, 1649, 1693
MaeIII GTNAC 7 cut(s) 190, 381, 532, 670, 709, 955, 1387
MalI GATC 6 cut(s) 450, 492, 519, 628, 1162, 1214
MboI GATC 6 cut(s) 448, 490, 517, 626, 1160, 1212
MflI RGATCY 2 cut(s) 517, 1212
MhlI GDGCHC 1 cut(s) 1848
MlsI TGGCCA 1 cut(s) 1071
MluNI TGGCCA 1 cut(s) 1071
MlyI GAGTC 2 cut(s) 50, 136
MmeI TCCRAC 1 cut(s) 1059
Mox20I TGGCCA 1 cut(s) 1071
Mph1103I ATGCAT 2 cut(s) 1023, 1429
MroI TCCGGA 1 cut(s) 1326
MscI TGGCCA 1 cut(s) 1071
MseI TTAA 8 cut(s) 138, 324, 885, 1037, 1280, 1535, 1608, 1775
MslI CAYNNNNRTG 1 cut(s) 1020
Msp20I TGGCCA 1 cut(s) 1071
MspCI CTTAAG 2 cut(s) 323, 1607
MspI CCGG 4 cut(s) 359, 1152, 1327, 1743
MspR9I CCNGG 5 cut(s) 282, 473, 594, 919, 1152
MvaI CCWGG 4 cut(s) 282, 473, 594, 919
MwoI GCNNNNNNNGC 4 cut(s) 1259, 1553, 1792, 1865
NciI CCSGG 1 cut(s) 1152
NdeII GATC 6 cut(s) 448, 490, 517, 626, 1160, 1212
NmuCI GTSAC 5 cut(s) 190, 381, 670, 709, 955
NsiI ATGCAT 2 cut(s) 1023, 1429
NspI RCATGY 4 cut(s) 420, 1021, 1025, 1605
NspV TTCGAA 1 cut(s) 171
PaeI GCATGC 1 cut(s) 1025
PagI TCATGA 1 cut(s) 1234
PaqCI CACCTGC 1 cut(s) 116
PceI AGGCCT 1 cut(s) 285
PfeI GAWTC 4 cut(s) 213, 815, 930, 1415
PflMI CCANNNNNTGG 1 cut(s) 1078
PinAI ACCGGT 1 cut(s) 1742
PkrI GCNGC 4 cut(s) 295, 1261, 1338, 1708
PleI GAGTC 2 cut(s) 50, 136
PpsI GAGTC 2 cut(s) 50, 136
Ppu21I YACGTR 1 cut(s) 1694
PsiI TTATAA 1 cut(s) 912
Psp124BI GAGCTC 1 cut(s) 1848
Psp6I CCWGG 4 cut(s) 280, 471, 592, 917
PspGI CCWGG 4 cut(s) 280, 471, 592, 917
PspPI GGNCC 3 cut(s) 110, 1295, 1852
PsuI RGATCY 2 cut(s) 517, 1212
RsaI GTAC 6 cut(s) 180, 1014, 1142, 1243, 1696, 1741
RsaNI GTAC 6 cut(s) 179, 1013, 1141, 1242, 1695, 1740
RseI CAYNNNNRTG 1 cut(s) 1020
SacI GAGCTC 1 cut(s) 1848
SaqAI TTAA 8 cut(s) 138, 324, 885, 1037, 1280, 1535, 1608, 1775
SatI GCNGC 4 cut(s) 294, 1260, 1337, 1707
Sau3AI GATC 6 cut(s) 448, 490, 517, 626, 1160, 1212
Sau96I GGNCC 3 cut(s) 110, 1295, 1852
ScaI AGTACT 1 cut(s) 1243
SchI GAGTC 2 cut(s) 50, 136
ScrFI CCNGG 5 cut(s) 282, 473, 594, 919, 1152
SduI GDGCHC 1 cut(s) 1848
SexAI ACCWGGT 2 cut(s) 592, 917
SfaNI GCATC 6 cut(s) 280, 322, 394, 739, 1240, 1543
SfcI CTRYAG 1 cut(s) 1764
SfuI TTCGAA 1 cut(s) 171
SinI GGWCC 1 cut(s) 1295
SmiMI CAYNNNNRTG 1 cut(s) 1020
SmlI CTYRAG 4 cut(s) 114, 323, 1607, 1847
SmoI CTYRAG 4 cut(s) 114, 323, 1607, 1847
SnaBI TACGTA 1 cut(s) 1694
SpeI ACTAGT 1 cut(s) 187
SphI GCATGC 1 cut(s) 1025
SseBI AGGCCT 1 cut(s) 285
SsiI CCGC 2 cut(s) 1675, 1841
SspMI CTAG 7 cut(s) 188, 287, 456, 753, 819, 1206, 1686
SstI GAGCTC 1 cut(s) 1848
StuI AGGCCT 1 cut(s) 285
StyD4I CCNGG 5 cut(s) 280, 471, 592, 917, 1150
StyI CCWWGG 1 cut(s) 353
TaaI ACNGT 4 cut(s) 17, 1060, 1241, 1765
TaiI ACGT 4 cut(s) 105, 197, 1652, 1696
TaqI TCGA 4 cut(s) 89, 171, 316, 333
TatI WGTACW 4 cut(s) 178, 1012, 1140, 1241
TfiI GAWTC 4 cut(s) 213, 815, 930, 1415
Tru1I TTAA 8 cut(s) 138, 324, 885, 1037, 1280, 1535, 1608, 1775
Tru9I TTAA 8 cut(s) 138, 324, 885, 1037, 1280, 1535, 1608, 1775
TseFI GTSAC 5 cut(s) 190, 381, 670, 709, 955
TseI GCWGC 4 cut(s) 293, 1259, 1336, 1706
Tsp45I GTSAC 5 cut(s) 190, 381, 670, 709, 955
TspDTI ATGAA 5 cut(s) 205, 402, 654, 869, 974
Van91I CCANNNNNTGG 1 cut(s) 1078
Vha464I CTTAAG 2 cut(s) 323, 1607
VpaK11BI GGWCC 1 cut(s) 1295
XapI RAATTY 4 cut(s) 91, 134, 166, 1665
XbaI TCTAGA 2 cut(s) 818, 1205
XceI RCATGY 4 cut(s) 420, 1021, 1025, 1605
XspI CTAG 7 cut(s) 188, 287, 456, 753, 819, 1206, 1686
ZraI GACGTC 1 cut(s) 1650
ZrmI AGTACT 1 cut(s) 1243
Zsp2I ATGCAT 2 cut(s) 1023, 1429
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.