Rw6G019280

Small RNA degrading nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0003406
Physical Location & Seq
Forward (+)
38493457 .. 38501067
7611 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G019280.1

Sequence Viewer

Length: 1713 bp
ATGCCTTCGTCCCACAGTAAATGCGAAGAAGAAGTTTTAGCTACCGAGGAAGACGACTCAAACAGAGACCAGAGCAATGGCAAAAGCTCGAATTTCTATGACGTTTATGGGCCTCAAGCTAAGGCAGGTGTGGAATTTAAGACTCCAGAAGCTACTTCAACTCTGAATTTCGAAGAGGTACAACAACTAGTGACGTGGGTGCTTGCTGATGGATTCATGCCGTCGTGGGTTTTTGTAAAGAACAAGCCCCTCATTCCCAAAGTGGTTATGCTTTATGTTCCAGGCCTAGATGCAGCTCTATACTTATCACAGCATCGACTACTTAAGGGTCTCGAAAAATGTTGTGGAAAACCAAGGCCTGTTTTAGCACTCAGCTATATGTCACATGAAAATCAGACGATTGATGCGCTCCTAACATGCAAACTGAAAAGAAAGAGGGAAGAGAGTGATCCAGCTAGGACAAAATCTAACCTGGAAACTCAACAAGATGATCCATCCTCTGTGGAACTTCTGAAAGATCTACCATTCCCAGTTACATTTTACACTCTTACAACAAAACAACTGGAAGACAATGGATATGTCTTCAATCAACCAGGTTTTCTTTCTACTCTTCCTGCTCCTTCTGGATCATCTCCTTATGAAATGTTAGCACTTGATTGTGAGATGTGTGTCACAGAAGAGGGTTTTGAGCTAACGAGAGCGACGCTTGTGACTATAAAAGGAGAGGTTGTGCTTGATAAATTGGTTAAACCAACAAATGCTATCAAAGATTATAACACCAGGTTTAGTGGAATCACACCTGAGATGTTAGATGGGGTGACTACAAGCCTCAAAGATATTCAGGAGGACTTCTTAAAGCTGGTTTACAAAGAGACCGTCCTAGTTGGCCATTCGTTGGAGAATGACTTATTAGCATTGAAAATTGCTCACAATCTCGTGATTGATACAGCAGTATTGTACAAGCACCCCGGTCAACGATCTTACAAATCTGCTCTTAGAGTTCTTGCCAAGAGATATCTTTCTAAAGAGATCCAGCAGTCAGGGAATGGTCATGACAGTACTGAAGATGCAAGAGCTGCTATGGAACTGGCGCTTTTAAAGATTAGACATGGACCTGACTTTGGCACAGCACCATCAGTTTTCCGGAAAAAGCTGCTAAAAGTTTTGAGCGATAATGGAAAGACCACCTCTATCATTGATGATGTTACCATTGTGAAGCGCTATGCCTCTGAATCATCCCATGCATTTCCTGTGGCCTCTGATGAGGAAGCTCTATCTAAAGCTGTAAAGGAGGCAAGGAACGAGAAAGTGCAGTTTGTCTGGACACAATTCTCAGAATTATATCGCTTTTTAAAGAATCAAGCAGAGGATGCTGAGAAATTAGATGTAAGACTTGCTCAGATGATAAGCTTGCTTACATGCCATAAGAAGTCAGGAGATAAAAAGGGTATTAGATGGAGTTTGACGTCTGAACTAAAGGAAATTCTTATCCGCGTGGACACTAGAATACGTGCTCTCTATGCTGCACTACCCACTAATGCCATGCTTATCATTTGTACCGGTCATGGAGATATAGCAACTGTAGATAGATTAAACAAAATGCTTTCGGAGCGTGAGGAAACCACAATGTGTCGGGATAGCATTGTAAAGGTCTTAGCGGAGCTCAAGGCCCAAGCTGAGGTAGCTTTATGTTTTGTGGGTGTGAAGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
RNase_T

Protein Analysis

570

Amino Acids

63.67

Weight (kDa)

6.2

Isoelectric Point (pI)

33.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_T PF00929 217 - 363 1.4e-11 Exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 774
AarI CACCTGC 1 cut(s) 116
AatII GACGTC 1 cut(s) 1469
Acc36I ACCTGC 1 cut(s) 116
AccB7I CCANNNNNTGG 1 cut(s) 895
AccII CGCG 1 cut(s) 1494
AccIII TCCGGA 1 cut(s) 1143
AciI CCGC 2 cut(s) 1492, 1658
AclWI GGATC 4 cut(s) 443, 485, 634, 1024
AcoI YGGCCR 1 cut(s) 886
AcsI RAATTY 4 cut(s) 91, 134, 166, 1482
AcuI CTGAAG 1 cut(s) 1083
AcyI GRCGYC 1 cut(s) 1466
AdeI CACNNNGTG 1 cut(s) 1629
AfaI GTAC 4 cut(s) 180, 959, 1060, 1558
AfeI AGCGCT 1 cut(s) 1220
AfiI CCNNNNNNNGG 3 cut(s) 895, 1121, 1678
AflII CTTAAG 1 cut(s) 323
AgeI ACCGGT 1 cut(s) 1559
AgsI TTSAA 3 cut(s) 159, 586, 919
AhlI ACTAGT 1 cut(s) 187
AjiI CACGTC 1 cut(s) 195
AjnI CCWGG 4 cut(s) 280, 471, 592, 779
Alw21I GWGCWC 2 cut(s) 1516, 1665
Alw26I GTCTC 3 cut(s) 60, 335, 866
AlwI GGATC 4 cut(s) 443, 485, 634, 1024
Aor13HI TCCGGA 1 cut(s) 1143
Aor51HI AGCGCT 1 cut(s) 1220
AoxI GGCC 6 cut(s) 110, 283, 356, 886, 1254, 1668
ApeKI GCWGC 4 cut(s) 293, 1076, 1153, 1523
ApoI RAATTY 4 cut(s) 91, 134, 166, 1482
ArsI GACNNNNNNTTYG 2 cut(s) 861, 893
AsiGI ACCGGT 1 cut(s) 1559
AspLEI GCGC 3 cut(s) 409, 1093, 1221
AspS9I GGNCC 3 cut(s) 110, 1112, 1669
AsuC2I CCSGG 1 cut(s) 969
AsuHPI GGTGA 1 cut(s) 829
AsuII TTCGAA 1 cut(s) 171
AvaII GGWCC 1 cut(s) 1112
BaeI ACNNNNGTAYC 2 cut(s) 936, 969
BalI TGGCCA 1 cut(s) 888
BanII GRGCYC 1 cut(s) 1665
BauI CACGAG 1 cut(s) 935
BbsI GAAGAC 3 cut(s) 57, 573, 574
Bbv12I GWGCWC 2 cut(s) 1516, 1665
BbvCI CCTCAGC 1 cut(s) 1677
BbvI GCAGC 4 cut(s) 305, 1063, 1140, 1510
BccI CCATC 5 cut(s) 203, 502, 806, 1141, 1449
BceAI ACGGC 1 cut(s) 205
BciT130I CCWGG 4 cut(s) 282, 473, 594, 781
BcnI CCSGG 1 cut(s) 969
BcoDI GTCTC 3 cut(s) 60, 335, 866
BcuI ACTAGT 1 cut(s) 187
BfaI CTAG 5 cut(s) 188, 287, 456, 881, 1503
BfmI CTRYAG 1 cut(s) 1581
BfoI RGCGCY 2 cut(s) 1094, 1222
BfrI CTTAAG 1 cut(s) 323
BfuAI ACCTGC 1 cut(s) 116
BglII AGATCT 1 cut(s) 517
BisI GCNGC 4 cut(s) 294, 1077, 1154, 1524
BlsI GCNGC 4 cut(s) 295, 1078, 1155, 1525
BmcAI AGTACT 1 cut(s) 1060
Bme1390I CCNGG 5 cut(s) 282, 473, 594, 781, 969
Bme18I GGWCC 1 cut(s) 1112
BmgBI CACGTC 1 cut(s) 195
BmgT120I GGNCC 3 cut(s) 110, 1112, 1669
BmrFI CCNGG 5 cut(s) 282, 473, 594, 781, 969
BmrI ACTGGG 1 cut(s) 524
BmsI GCATC 5 cut(s) 280, 322, 394, 1057, 1360
BmuI ACTGGG 1 cut(s) 524
BpiI GAAGAC 3 cut(s) 57, 573, 574
BpmI CTGGAG 1 cut(s) 129
Bpu10I CCTNAGC 2 cut(s) 120, 1677
Bpu14I TTCGAA 1 cut(s) 171
BpuEI CTTGAG 2 cut(s) 99, 1649
BpuMI CCSGG 1 cut(s) 969
BsaAI YACGTR 1 cut(s) 1511
BsaHI GRCGYC 1 cut(s) 1466
BsaI GGTCTC 3 cut(s) 60, 335, 866
BsaJI CCNNGG 3 cut(s) 45, 353, 967
BsaWI WCCGGW 2 cut(s) 1143, 1559
BsaXI ACNNNNNCTCC 2 cut(s) 714, 744
Bsc4I CCNNNNNNNGG 3 cut(s) 895, 1121, 1678
Bse118I RCCGGY 1 cut(s) 1559
Bse1I ACTGG 3 cut(s) 530, 567, 1092
Bse3DI GCAATG 1 cut(s) 82
BseAI TCCGGA 1 cut(s) 1143
BseBI CCWGG 4 cut(s) 282, 473, 594, 781
BseDI CCNNGG 3 cut(s) 45, 353, 967
BseGI GGATG 3 cut(s) 494, 1235, 1375
BseLI CCNNNNNNNGG 3 cut(s) 895, 1121, 1678
BseMI GCAATG 1 cut(s) 82
BseMII CTCAG 6 cut(s) 385, 792, 1347, 1365, 1412, 1668
BseNI ACTGG 3 cut(s) 530, 567, 1092
BseXI GCAGC 4 cut(s) 305, 1063, 1140, 1510
BsgI GTGCAG 2 cut(s) 1331, 1509
Bsh1236I CGCG 1 cut(s) 1494
BshFI GGCC 6 cut(s) 112, 285, 358, 888, 1256, 1670
BshTI ACCGGT 1 cut(s) 1559
BsiHKAI GWGCWC 2 cut(s) 1516, 1665
BsiSI CCGG 3 cut(s) 969, 1144, 1560
BslI CCNNNNNNNGG 3 cut(s) 895, 1121, 1678
BsmAI GTCTC 3 cut(s) 60, 335, 866
BsnI GGCC 6 cut(s) 112, 285, 358, 888, 1256, 1670
Bso31I GGTCTC 3 cut(s) 60, 335, 866
Bsp119I TTCGAA 1 cut(s) 171
Bsp1286I GDGCHC 2 cut(s) 1516, 1665
Bsp13I TCCGGA 1 cut(s) 1143
Bsp1407I TGTACA 1 cut(s) 957
Bsp143I GATC 6 cut(s) 448, 490, 517, 626, 977, 1029
BspACI CCGC 2 cut(s) 1492, 1658
BspANI GGCC 6 cut(s) 112, 285, 358, 888, 1256, 1670
BspCNI CTCAG 6 cut(s) 384, 793, 1346, 1366, 1411, 1669
BspEI TCCGGA 1 cut(s) 1143
BspFNI CGCG 1 cut(s) 1494
BspHI TCATGA 1 cut(s) 1051
BspMI ACCTGC 1 cut(s) 116
BspPI GGATC 4 cut(s) 443, 485, 634, 1024
BspT104I TTCGAA 1 cut(s) 171
BspTI CTTAAG 1 cut(s) 323
BspTNI GGTCTC 3 cut(s) 60, 335, 866
BsrDI GCAATG 1 cut(s) 82
BsrFI RCCGGY 1 cut(s) 1559
BsrGI TGTACA 1 cut(s) 957
BsrI ACTGG 3 cut(s) 530, 567, 1092
BssAI RCCGGY 1 cut(s) 1559
BssECI CCNNGG 3 cut(s) 45, 353, 967
BssMI GATC 6 cut(s) 448, 490, 517, 626, 977, 1029
BssNI GRCGYC 1 cut(s) 1466
BssSI CACGAG 1 cut(s) 935
BssT1I CCWWGG 1 cut(s) 353
Bst2BI CACGAG 1 cut(s) 935
Bst2UI CCWGG 4 cut(s) 282, 473, 594, 781
Bst4CI ACNGT 4 cut(s) 17, 877, 1058, 1582
Bst6I CTCTTC 5 cut(s) 168, 435, 615, 672, 1700
BstACI GRCGYC 1 cut(s) 1466
BstAFI CTTAAG 1 cut(s) 323
BstAPI GCANNNNNTGC 2 cut(s) 1076, 1370
BstAUI TGTACA 1 cut(s) 957
BstBAI YACGTR 1 cut(s) 1511
BstBI TTCGAA 1 cut(s) 171
BstC8I GCNNGC 2 cut(s) 204, 1412
BstDEI CTNAG 9 cut(s) 120, 371, 801, 995, 1333, 1374, 1398, 1654, 1677
BstF5I GGATG 3 cut(s) 494, 1235, 1375
BstFNI CGCG 1 cut(s) 1494
BstH2I RGCGCY 2 cut(s) 1094, 1222
BstHHI GCGC 3 cut(s) 409, 1093, 1221
BstKTI GATC 6 cut(s) 451, 493, 520, 629, 980, 1032
BstMAI GTCTC 3 cut(s) 60, 335, 866
BstMBI GATC 6 cut(s) 448, 490, 517, 626, 977, 1029
BstMWI GCNNNNNNNGC 5 cut(s) 1076, 1370, 1520, 1609, 1682
BstNI CCWGG 4 cut(s) 282, 473, 594, 781
BstNSI RCATGY 2 cut(s) 420, 1422
BstSCI CCNGG 5 cut(s) 280, 471, 592, 779, 967
BstSFI CTRYAG 1 cut(s) 1581
BstUI CGCG 1 cut(s) 1494
BstV1I GCAGC 4 cut(s) 305, 1063, 1140, 1510
BstV2I GAAGAC 3 cut(s) 57, 573, 574
BstX2I RGATCY 2 cut(s) 517, 1029
BstXI CCANNNNNNTGG 1 cut(s) 77
BstYI RGATCY 2 cut(s) 517, 1029
BsuRI GGCC 6 cut(s) 112, 285, 358, 888, 1256, 1670
BtrI CACGTC 1 cut(s) 195
BtsCI GGATG 3 cut(s) 494, 1235, 1375
BveI ACCTGC 1 cut(s) 116
Cac8I GCNNGC 2 cut(s) 204, 1412
CciI TCATGA 1 cut(s) 1051
CfoI GCGC 3 cut(s) 409, 1093, 1221
Cfr10I RCCGGY 1 cut(s) 1559
Cfr13I GGNCC 3 cut(s) 110, 1112, 1669
CseI GACGC 1 cut(s) 712
CsiI ACCWGGT 2 cut(s) 592, 779
Csp6I GTAC 4 cut(s) 179, 958, 1059, 1557
CspAI ACCGGT 1 cut(s) 1559
CviAII CATG 9 cut(s) 217, 386, 417, 1052, 1109, 1241, 1419, 1543, 1565
CviQI GTAC 4 cut(s) 179, 958, 1059, 1557
DdeI CTNAG 9 cut(s) 120, 371, 801, 995, 1333, 1374, 1398, 1654, 1677
DpnI GATC 6 cut(s) 450, 492, 519, 628, 979, 1031
DpnII GATC 6 cut(s) 448, 490, 517, 626, 977, 1029
DraI TTTAAA 2 cut(s) 1098, 1353
DraIII CACNNNGTG 1 cut(s) 1629
EaeI YGGCCR 1 cut(s) 886
Eam1104I CTCTTC 5 cut(s) 168, 435, 615, 672, 1700
EarI CTCTTC 5 cut(s) 168, 435, 615, 672, 1700
Ecl136II GAGCTC 1 cut(s) 1663
Eco130I CCWWGG 1 cut(s) 353
Eco147I AGGCCT 2 cut(s) 285, 358
Eco24I GRGCYC 1 cut(s) 1665
Eco31I GGTCTC 3 cut(s) 60, 335, 866
Eco32I GATATC 1 cut(s) 1016
Eco47I GGWCC 1 cut(s) 1112
Eco47III AGCGCT 1 cut(s) 1220
Eco53kI GAGCTC 1 cut(s) 1663
Eco57I CTGAAG 1 cut(s) 1083
EcoICRI GAGCTC 1 cut(s) 1663
EcoRII CCWGG 4 cut(s) 280, 471, 592, 779
EcoRV GATATC 1 cut(s) 1016
EcoT14I CCWWGG 1 cut(s) 353
EcoT22I ATGCAT 1 cut(s) 1246
EcoT38I GRGCYC 1 cut(s) 1665
ErhI CCWWGG 1 cut(s) 353
FaeI CATG 9 cut(s) 220, 389, 420, 1055, 1112, 1244, 1422, 1546, 1568
FatI CATG 9 cut(s) 216, 385, 416, 1051, 1108, 1240, 1418, 1542, 1564
Fnu4HI GCNGC 4 cut(s) 294, 1077, 1154, 1524
FokI GGATG 3 cut(s) 481, 1222, 1382
FriOI GRGCYC 1 cut(s) 1665
Fsp4HI GCNGC 4 cut(s) 294, 1077, 1154, 1524
FspBI CTAG 5 cut(s) 188, 287, 456, 881, 1503
GlaI GCGC 3 cut(s) 408, 1092, 1220
GluI GCNGC 4 cut(s) 294, 1077, 1154, 1524
GsuI CTGGAG 1 cut(s) 129
HaeII RGCGCY 2 cut(s) 1094, 1222
HaeIII GGCC 6 cut(s) 112, 285, 358, 888, 1256, 1670
HapII CCGG 3 cut(s) 969, 1144, 1560
HgaI GACGC 1 cut(s) 712
HhaI GCGC 3 cut(s) 409, 1093, 1221
Hin1I GRCGYC 1 cut(s) 1466
Hin1II CATG 9 cut(s) 220, 389, 420, 1055, 1112, 1244, 1422, 1546, 1568
Hin6I GCGC 3 cut(s) 407, 1091, 1219
HinP1I GCGC 3 cut(s) 407, 1091, 1219
HincII GTYRAC 1 cut(s) 974
HindII GTYRAC 1 cut(s) 974
HindIII AAGCTT 1 cut(s) 1408
HinfI GANTC 6 cut(s) 56, 142, 213, 792, 1232, 1357
HpaII CCGG 3 cut(s) 969, 1144, 1560
HphI GGTGA 1 cut(s) 829
Hpy166II GTNNAC 3 cut(s) 865, 974, 1498
Hpy188I TCNGA 9 cut(s) 165, 396, 513, 1231, 1261, 1336, 1401, 1471, 1609
Hpy8I GTNNAC 3 cut(s) 865, 974, 1498
Hpy99I CGWCG 2 cut(s) 226, 706
HpyAV CCTTC 2 cut(s) 15, 630
HpyCH4III ACNGT 4 cut(s) 17, 877, 1058, 1582
HpyCH4IV ACGT 4 cut(s) 102, 194, 1466, 1510
HpyCH4V TGCA 6 cut(s) 293, 420, 1070, 1244, 1312, 1526
HpyF10VI GCNNNNNNNGC 5 cut(s) 1076, 1370, 1520, 1609, 1682
HpyF3I CTNAG 9 cut(s) 120, 371, 801, 995, 1333, 1374, 1398, 1654, 1677
HpySE526I ACGT 4 cut(s) 102, 194, 1466, 1510
Hsp92I GRCGYC 1 cut(s) 1466
Hsp92II CATG 9 cut(s) 220, 389, 420, 1055, 1112, 1244, 1422, 1546, 1568
HspAI GCGC 3 cut(s) 407, 1091, 1219
Kpn2I TCCGGA 1 cut(s) 1143
Kzo9I GATC 6 cut(s) 448, 490, 517, 626, 977, 1029
LmnI GCTCC 4 cut(s) 414, 622, 1609, 1660
Lsp1109I GCAGC 4 cut(s) 305, 1063, 1140, 1510
LweI GCATC 5 cut(s) 280, 322, 394, 1057, 1360
MabI ACCWGGT 2 cut(s) 592, 779
MaeI CTAG 5 cut(s) 188, 287, 456, 881, 1503
MaeII ACGT 4 cut(s) 102, 194, 1466, 1510
MaeIII GTNAC 7 cut(s) 190, 381, 532, 670, 709, 817, 1204
MalI GATC 6 cut(s) 450, 492, 519, 628, 979, 1031
MboI GATC 6 cut(s) 448, 490, 517, 626, 977, 1029
MflI RGATCY 2 cut(s) 517, 1029
MhlI GDGCHC 2 cut(s) 1516, 1665
MlsI TGGCCA 1 cut(s) 888
MluCI AATT 9 cut(s) 91, 134, 166, 740, 921, 1328, 1337, 1379, 1482
MluNI TGGCCA 1 cut(s) 888
MlyI GAGTC 2 cut(s) 50, 136
MmeI TCCRAC 1 cut(s) 876
Mox20I TGGCCA 1 cut(s) 888
Mph1103I ATGCAT 1 cut(s) 1246
MroI TCCGGA 1 cut(s) 1143
MscI TGGCCA 1 cut(s) 888
MseI TTAA 7 cut(s) 138, 324, 747, 854, 1097, 1352, 1592
Msp20I TGGCCA 1 cut(s) 888
MspCI CTTAAG 1 cut(s) 323
MspI CCGG 3 cut(s) 969, 1144, 1560
MspR9I CCNGG 5 cut(s) 282, 473, 594, 781, 969
MvaI CCWGG 4 cut(s) 282, 473, 594, 781
MvnI CGCG 1 cut(s) 1494
MwoI GCNNNNNNNGC 5 cut(s) 1076, 1370, 1520, 1609, 1682
NciI CCSGG 1 cut(s) 969
NdeII GATC 6 cut(s) 448, 490, 517, 626, 977, 1029
NlaIII CATG 9 cut(s) 220, 389, 420, 1055, 1112, 1244, 1422, 1546, 1568
NmuCI GTSAC 5 cut(s) 190, 381, 670, 709, 817
NsiI ATGCAT 1 cut(s) 1246
NspI RCATGY 2 cut(s) 420, 1422
NspV TTCGAA 1 cut(s) 171
PagI TCATGA 1 cut(s) 1051
PaqCI CACCTGC 1 cut(s) 116
PceI AGGCCT 2 cut(s) 285, 358
PfeI GAWTC 4 cut(s) 213, 792, 1232, 1357
PflMI CCANNNNNTGG 1 cut(s) 895
PinAI ACCGGT 1 cut(s) 1559
PkrI GCNGC 4 cut(s) 295, 1078, 1155, 1525
PleI GAGTC 2 cut(s) 50, 136
PpsI GAGTC 2 cut(s) 50, 136
Ppu21I YACGTR 1 cut(s) 1511
PsiI TTATAA 1 cut(s) 774
Psp124BI GAGCTC 1 cut(s) 1665
Psp6I CCWGG 4 cut(s) 280, 471, 592, 779
PspGI CCWGG 4 cut(s) 280, 471, 592, 779
PspPI GGNCC 3 cut(s) 110, 1112, 1669
PsuI RGATCY 2 cut(s) 517, 1029
RsaI GTAC 4 cut(s) 180, 959, 1060, 1558
RsaNI GTAC 4 cut(s) 179, 958, 1059, 1557
SacI GAGCTC 1 cut(s) 1665
SaqAI TTAA 7 cut(s) 138, 324, 747, 854, 1097, 1352, 1592
SatI GCNGC 4 cut(s) 294, 1077, 1154, 1524
Sau3AI GATC 6 cut(s) 448, 490, 517, 626, 977, 1029
Sau96I GGNCC 3 cut(s) 110, 1112, 1669
ScaI AGTACT 1 cut(s) 1060
SchI GAGTC 2 cut(s) 50, 136
ScrFI CCNGG 5 cut(s) 282, 473, 594, 781, 969
SduI GDGCHC 2 cut(s) 1516, 1665
SexAI ACCWGGT 2 cut(s) 592, 779
SfaNI GCATC 5 cut(s) 280, 322, 394, 1057, 1360
SfcI CTRYAG 1 cut(s) 1581
SfuI TTCGAA 1 cut(s) 171
SinI GGWCC 1 cut(s) 1112
SmlI CTYRAG 3 cut(s) 114, 323, 1664
SmoI CTYRAG 3 cut(s) 114, 323, 1664
SpeI ACTAGT 1 cut(s) 187
Sse9I AATT 9 cut(s) 91, 134, 166, 740, 921, 1328, 1337, 1379, 1482
SseBI AGGCCT 2 cut(s) 285, 358
SsiI CCGC 2 cut(s) 1492, 1658
SspMI CTAG 5 cut(s) 188, 287, 456, 881, 1503
SstI GAGCTC 1 cut(s) 1665
StuI AGGCCT 2 cut(s) 285, 358
StyD4I CCNGG 5 cut(s) 280, 471, 592, 779, 967
StyI CCWWGG 1 cut(s) 353
TaaI ACNGT 4 cut(s) 17, 877, 1058, 1582
TaiI ACGT 4 cut(s) 105, 197, 1469, 1513
TaqI TCGA 4 cut(s) 89, 171, 316, 333
TasI AATT 9 cut(s) 91, 134, 166, 740, 921, 1328, 1337, 1379, 1482
TatI WGTACW 2 cut(s) 957, 1058
TfiI GAWTC 4 cut(s) 213, 792, 1232, 1357
Tru1I TTAA 7 cut(s) 138, 324, 747, 854, 1097, 1352, 1592
Tru9I TTAA 7 cut(s) 138, 324, 747, 854, 1097, 1352, 1592
TseFI GTSAC 5 cut(s) 190, 381, 670, 709, 817
TseI GCWGC 4 cut(s) 293, 1076, 1153, 1523
Tsp45I GTSAC 5 cut(s) 190, 381, 670, 709, 817
TspDTI ATGAA 3 cut(s) 205, 402, 654
Van91I CCANNNNNTGG 1 cut(s) 895
Vha464I CTTAAG 1 cut(s) 323
VpaK11BI GGWCC 1 cut(s) 1112
XapI RAATTY 4 cut(s) 91, 134, 166, 1482
XceI RCATGY 2 cut(s) 420, 1422
XspI CTAG 5 cut(s) 188, 287, 456, 881, 1503
ZraI GACGTC 1 cut(s) 1467
ZrmI AGTACT 1 cut(s) 1060
Zsp2I ATGCAT 1 cut(s) 1246
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.