AT5G43090

Encodes a member of the Arabidopsis Pumilio (APUM) proteins containing PUF domain (eight repeats of approximately 36 amino acids each). PUF proteins regulate both mRNA stability and translation through sequence-specific binding to the 3' UTR of target mRNA transcripts

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0004567
Physical Location & Seq
Forward (+)
17295933 .. 17298214
2282 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G43090.1

Sequence Viewer

Length: 1584 bp
ATGGATAACAAGTTTTATGTGAACACTAACGGCGAACGCAACATCTGGTCCACGGCGGCGGAGGAGAATATGACAACAGCGGCGTCATCGTCTCAGTCACAACCGCCGCAGATGCAGTCGTCTAAGTTTCATCAACCGGAGAACCACATCCATATAAATGATGGTTTCTCGAGTGGTGCTTTTGATTTGCAAACCCTAGAATCCTCTTTTCGTGGACTTTCTTTTGCAGATTCAAATGTTCATCAGAACGGTAATCCGATACTCCCCGCCCATCAATACAATCAGGTGTTTAACGGTGGAGGTGGTAGTTATGGTGATGGGTACTTATTTCCTCCAAGTGGTTCTTATCACCACTATGAATTAAAAGACTTGCAAAGGTTTAACCAGAATCAACAGAGATTGTCATATCAGAACGATTATGTTAATCAATCTTATAGGTATGATACTATTGGTGGTGGTAATGGGATGCTTAACAATAGTTTCTTGAATGGAGTTCCTTGTGCTTCAAGAAACAATGTTTCAGATTACTACACTAATATTTTTGGGTATGGAGTTAACAATTCTTGGAGGAGTAATGAAGGTTGTACCTATAATCAAGATCAGGCGGCCTCGTCGATGGAGAATGGGAGGGGCTCGTACTTTTCCATTGCTACAGACCGAGTATGGAGCAAGGAATTGGAGAAGACTATCTTTGTGGGTACTAAAGAGACGATTGATATGATTTTCGATGGTCTCATTGTCGGTATTTGTGAGTTGATGGTTGATCCTTTTGGTAATGATGTTGTTAAGCTGCTTATCGGAAAATGCAGCTCTGAACAGATCATTCTGATTGTTGATGTTGTCACTCGGCACATTTCTAAGTTTGTTAACATATGCTTTAATCCGATCGGAACTTTGGCAATACAAGTTCTGTTAACTAGTATTCATGAGCGTGCTAACAATCAAATCCCGCGCATTATGGATGCTATAAGCTCGGTAGCACTTCAATTGACAAGGAACACCAATGCCAAATATGTGATTCTAGCATGTTTCCGCATGTTTACTTCGTCGCAGTGTAGGCGTCTTCTTGAAGTTGTCTCTCAACATTGTTACCAGATAGCGATTGATCAAAACGGATGCTGCTTGCTTCAGCAATGTTTTGACAAGGAACGTGTTCCGAATCATGAGATAAGGCAACGTTTGATTTCGGAAGTTATCGAACATGCGTTGAAACTCTGCTTGAACTGTCATGGAAACTATGTGGTTCAATATGTCGTGGAGCTCGATAATCAACATGAAACAGATTTGCTCGTGAATAAGCTACTTAGGAACTATGCGCATCTAGCCCGCAACAAATATGGAAGTCATGTTGTGCAGAAGTTGTTGAAACTAAGGGGTATCGACTCTAAGCTGATTGTTGTTGACTTGCTTAGAGGGATTGATACACTTCTTCTTGACCCGTTTGGTAACTATGTCATTCAAACCGCATGGTTTGTGTCTAAGGAAGATGTGCGCCAGATGTTGAGGTATTATATAGAGCGTAACATTCGATTGATGAGGTGCAACAAATTTGGGAACAAAATTCTGGAGAAACTCAATATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUF

Protein Analysis

527

Amino Acids

59.94

Weight (kDa)

6.96

Isoelectric Point (pI)

40.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUF_NOP9 PF22493 216 - 457 2.8e-10 NOP9-like PUF repeat domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1317
AccII CGCG 1 cut(s) 952
AclI AACGTT 1 cut(s) 1177
AclWI GGATC 1 cut(s) 758
AcsI RAATTY 2 cut(s) 1547, 1560
AcuI CTGAAG 1 cut(s) 1112
AcyI GRCGYC 2 cut(s) 83, 1060
AfaI GTAC 4 cut(s) 323, 586, 638, 700
AfiI CCNNNNNNNGG 1 cut(s) 338
AflIII ACRYGT 1 cut(s) 1150
AhlI ACTAGT 1 cut(s) 917
AluBI AGCT 6 cut(s) 790, 810, 972, 1261, 1300, 1390
AluI AGCT 6 cut(s) 790, 810, 972, 1261, 1300, 1390
Alw21I GWGCWC 1 cut(s) 1263
Alw26I GTCTC 4 cut(s) 96, 701, 737, 1081
AlwI GGATC 1 cut(s) 758
Ama87I CYCGRG 1 cut(s) 169
AoxI GGCC 1 cut(s) 606
ApeKI GCWGC 3 cut(s) 790, 807, 1119
ApoI RAATTY 2 cut(s) 1547, 1560
Asp700I GAANNNNTTC 1 cut(s) 1152
AspLEI GCGC 3 cut(s) 954, 1318, 1494
AspS9I GGNCC 1 cut(s) 48
AsuHPI GGTGA 2 cut(s) 326, 341
AvaI CYCGRG 1 cut(s) 169
AvaII GGWCC 1 cut(s) 48
BanII GRGCYC 2 cut(s) 635, 1263
BauI CACGAG 1 cut(s) 1289
BbsI GAAGAC 2 cut(s) 689, 1055
Bbv12I GWGCWC 1 cut(s) 1263
BbvI GCAGC 3 cut(s) 777, 819, 1106
BccI CCATC 6 cut(s) 155, 279, 311, 610, 722, 751
BceAI ACGGC 2 cut(s) 46, 69
BclI TGATCA 1 cut(s) 1105
BcoDI GTCTC 4 cut(s) 96, 701, 737, 1081
BcuI ACTAGT 1 cut(s) 917
BfaI CTAG 5 cut(s) 197, 918, 1022, 1322, 1582
BfmI CTRYAG 1 cut(s) 651
BisI GCNGC 7 cut(s) 57, 81, 107, 606, 791, 808, 1120
BlsI GCNGC 7 cut(s) 58, 82, 108, 607, 792, 809, 1121
Bme18I GGWCC 1 cut(s) 48
BmeT110I CYCGRG 1 cut(s) 169
BmgT120I GGNCC 1 cut(s) 48
BmsI GCATC 5 cut(s) 102, 456, 952, 1106, 1327
BpiI GAAGAC 2 cut(s) 689, 1055
BsaHI GRCGYC 2 cut(s) 83, 1060
BsaI GGTCTC 1 cut(s) 737
BsaJI CCNNGG 1 cut(s) 51
BsaWI WCCGGW 1 cut(s) 136
Bsc4I CCNNNNNNNGG 1 cut(s) 338
Bse3DI GCAATG 2 cut(s) 645, 1139
BseDI CCNNGG 1 cut(s) 51
BseGI GGATG 4 cut(s) 147, 471, 967, 1121
BseLI CCNNNNNNNGG 1 cut(s) 338
BseMI GCAATG 2 cut(s) 645, 1139
BseMII CTCAG 1 cut(s) 107
BseRI GAGGAG 2 cut(s) 77, 583
BseXI GCAGC 3 cut(s) 777, 819, 1106
BsgI GTGCAG 1 cut(s) 1373
Bsh1236I CGCG 1 cut(s) 952
Bsh1285I CGRYCG 1 cut(s) 888
BshFI GGCC 1 cut(s) 608
BsiEI CGRYCG 1 cut(s) 888
BsiHKAI GWGCWC 1 cut(s) 1263
BsiHKCI CYCGRG 1 cut(s) 169
BsiSI CCGG 1 cut(s) 137
BslI CCNNNNNNNGG 1 cut(s) 338
BsmAI GTCTC 4 cut(s) 96, 701, 737, 1081
BsmBI CGTCTC 2 cut(s) 96, 701
BsnI GGCC 1 cut(s) 608
Bso31I GGTCTC 1 cut(s) 737
BsoBI CYCGRG 1 cut(s) 169
Bsp1286I GDGCHC 2 cut(s) 635, 1263
Bsp143I GATC 5 cut(s) 598, 763, 819, 885, 1105
BspANI GGCC 1 cut(s) 608
BspCNI CTCAG 1 cut(s) 106
BspFNI CGCG 1 cut(s) 952
BspHI TCATGA 2 cut(s) 925, 1162
BspPI GGATC 1 cut(s) 758
BspTNI GGTCTC 1 cut(s) 737
BsrDI GCAATG 2 cut(s) 645, 1139
BssECI CCNNGG 1 cut(s) 51
BssMI GATC 5 cut(s) 598, 763, 819, 885, 1105
BssNI GRCGYC 2 cut(s) 83, 1060
BssSI CACGAG 1 cut(s) 1289
Bst2BI CACGAG 1 cut(s) 1289
Bst4CI ACNGT 3 cut(s) 251, 296, 1226
BstACI GRCGYC 2 cut(s) 83, 1060
BstC8I GCNNGC 3 cut(s) 933, 1124, 1327
BstDEI CTNAG 8 cut(s) 93, 123, 858, 1304, 1370, 1386, 1409, 1479
BstDSI CCRYGG 1 cut(s) 51
BstF5I GGATG 4 cut(s) 147, 471, 967, 1121
BstFNI CGCG 1 cut(s) 952
BstHHI GCGC 3 cut(s) 954, 1318, 1494
BstKTI GATC 5 cut(s) 601, 766, 822, 888, 1108
BstMAI GTCTC 4 cut(s) 96, 701, 737, 1081
BstMBI GATC 5 cut(s) 598, 763, 819, 885, 1105
BstMCI CGRYCG 1 cut(s) 888
BstMWI GCNNNNNNNGC 3 cut(s) 112, 1057, 1322
BstNSI RCATGY 3 cut(s) 1029, 1039, 1205
BstSFI CTRYAG 1 cut(s) 651
BstUI CGCG 1 cut(s) 952
BstV1I GCAGC 3 cut(s) 777, 819, 1106
BstV2I GAAGAC 2 cut(s) 689, 1055
BsuRI GGCC 1 cut(s) 608
BtgI CCRYGG 1 cut(s) 51
BtsCI GGATG 4 cut(s) 147, 471, 967, 1121
BtsI GCAGTG 1 cut(s) 1058
BtsIMutI CAGTG 1 cut(s) 1058
Cac8I GCNNGC 3 cut(s) 933, 1124, 1327
CciI TCATGA 2 cut(s) 925, 1162
CfoI GCGC 3 cut(s) 954, 1318, 1494
Cfr13I GGNCC 1 cut(s) 48
CseI GACGC 2 cut(s) 72, 1049
Csp6I GTAC 4 cut(s) 322, 585, 637, 699
CviAII CATG 9 cut(s) 926, 1026, 1036, 1163, 1202, 1229, 1274, 1346, 1467
CviJI RGCY 9 cut(s) 608, 633, 790, 810, 972, 1261, 1300, 1325, 1390
CviKI_1 RGCY 9 cut(s) 608, 633, 790, 810, 972, 1261, 1300, 1325, 1390
CviQI GTAC 4 cut(s) 322, 585, 637, 699
DdeI CTNAG 8 cut(s) 93, 123, 858, 1304, 1370, 1386, 1409, 1479
DpnI GATC 5 cut(s) 600, 765, 821, 887, 1107
DpnII GATC 5 cut(s) 598, 763, 819, 885, 1105
EciI GGCGGA 1 cut(s) 74
Ecl136II GAGCTC 1 cut(s) 1261
Eco24I GRGCYC 2 cut(s) 635, 1263
Eco31I GGTCTC 1 cut(s) 737
Eco47I GGWCC 1 cut(s) 48
Eco53kI GAGCTC 1 cut(s) 1261
Eco57I CTGAAG 1 cut(s) 1112
Eco88I CYCGRG 1 cut(s) 169
EcoICRI GAGCTC 1 cut(s) 1261
EcoT38I GRGCYC 2 cut(s) 635, 1263
Esp3I CGTCTC 2 cut(s) 96, 701
FaeI CATG 9 cut(s) 929, 1029, 1039, 1166, 1205, 1232, 1277, 1349, 1470
FalI AAGNNNNNCTT 4 cut(s) 328, 360, 674, 706
FatI CATG 9 cut(s) 925, 1025, 1035, 1162, 1201, 1228, 1273, 1345, 1466
FauI CCCGC 3 cut(s) 274, 957, 1334
FauNDI CATATG 1 cut(s) 872
FbaI TGATCA 1 cut(s) 1105
Fnu4HI GCNGC 7 cut(s) 57, 81, 107, 606, 791, 808, 1120
FokI GGATG 4 cut(s) 134, 478, 974, 1128
FriOI GRGCYC 2 cut(s) 635, 1263
Fsp4HI GCNGC 7 cut(s) 57, 81, 107, 606, 791, 808, 1120
FspAI RTGCGCAY 1 cut(s) 1317
FspBI CTAG 5 cut(s) 197, 918, 1022, 1322, 1582
FspI TGCGCA 1 cut(s) 1317
GlaI GCGC 3 cut(s) 953, 1317, 1493
GluI GCNGC 7 cut(s) 57, 81, 107, 606, 791, 808, 1120
HaeIII GGCC 1 cut(s) 608
HapII CCGG 1 cut(s) 137
HgaI GACGC 2 cut(s) 72, 1049
HhaI GCGC 3 cut(s) 954, 1318, 1494
Hin1I GRCGYC 2 cut(s) 83, 1060
Hin1II CATG 9 cut(s) 929, 1029, 1039, 1166, 1205, 1232, 1277, 1349, 1470
Hin6I GCGC 3 cut(s) 952, 1316, 1492
HinP1I GCGC 3 cut(s) 952, 1316, 1492
HincII GTYRAC 4 cut(s) 556, 868, 915, 1402
HindII GTYRAC 4 cut(s) 556, 868, 915, 1402
HinfI GANTC 6 cut(s) 200, 230, 388, 1018, 1159, 1382
HpaI GTTAAC 3 cut(s) 556, 868, 915
HpaII CCGG 1 cut(s) 137
HphI GGTGA 2 cut(s) 326, 341
Hpy166II GTNNAC 8 cut(s) 22, 51, 215, 556, 868, 915, 1041, 1402
Hpy8I GTNNAC 8 cut(s) 22, 51, 215, 556, 868, 915, 1041, 1402
Hpy99I CGWCG 2 cut(s) 616, 1051
HpyAV CCTTC 1 cut(s) 572
HpyCH4III ACNGT 3 cut(s) 251, 296, 1226
HpyCH4IV ACGT 2 cut(s) 1150, 1177
HpyCH4V TGCA 7 cut(s) 115, 190, 227, 373, 807, 1354, 1542
HpyF10VI GCNNNNNNNGC 3 cut(s) 112, 1057, 1322
HpyF3I CTNAG 8 cut(s) 93, 123, 858, 1304, 1370, 1386, 1409, 1479
HpySE526I ACGT 2 cut(s) 1150, 1177
Hsp92I GRCGYC 2 cut(s) 83, 1060
Hsp92II CATG 9 cut(s) 929, 1029, 1039, 1166, 1205, 1232, 1277, 1349, 1470
HspAI GCGC 3 cut(s) 952, 1316, 1492
Ksp22I TGATCA 1 cut(s) 1105
KspAI GTTAAC 3 cut(s) 556, 868, 915
Kzo9I GATC 5 cut(s) 598, 763, 819, 885, 1105
LmnI GCTCC 2 cut(s) 666, 1258
LpnPI CCDG 8 cut(s) 31, 150, 269, 398, 587, 1106, 1508, 1550
Lsp1109I GCAGC 3 cut(s) 777, 819, 1106
LweI GCATC 5 cut(s) 102, 456, 952, 1106, 1327
MaeI CTAG 5 cut(s) 197, 918, 1022, 1322, 1582
MaeII ACGT 2 cut(s) 1150, 1177
MaeIII GTNAC 5 cut(s) 96, 841, 1088, 1445, 1520
MalI GATC 5 cut(s) 600, 765, 821, 887, 1107
MboI GATC 5 cut(s) 598, 763, 819, 885, 1105
MboII GAAGA 4 cut(s) 694, 1055, 1421, 1496
MfeI CAATTG 1 cut(s) 986
MhlI GDGCHC 2 cut(s) 635, 1263
MluCI AATT 6 cut(s) 359, 559, 674, 986, 1547, 1560
MlyI GAGTC 1 cut(s) 1376
MroXI GAANNNNTTC 1 cut(s) 1152
MslI CAYNNNNRTG 3 cut(s) 156, 354, 930
MspA1I CMGCKG 1 cut(s) 80
MspI CCGG 1 cut(s) 137
MunI CAATTG 1 cut(s) 986
MvnI CGCG 1 cut(s) 952
MwoI GCNNNNNNNGC 3 cut(s) 112, 1057, 1322
NdeI CATATG 1 cut(s) 872
NdeII GATC 5 cut(s) 598, 763, 819, 885, 1105
NlaIII CATG 9 cut(s) 929, 1029, 1039, 1166, 1205, 1232, 1277, 1349, 1470
NmeAIII GCCGAG 1 cut(s) 826
NmuCI GTSAC 2 cut(s) 96, 841
NsbI TGCGCA 1 cut(s) 1317
NspI RCATGY 3 cut(s) 1029, 1039, 1205
PaeR7I CTCGAG 1 cut(s) 169
PagI TCATGA 2 cut(s) 925, 1162
PcsI WCGNNNNNNNCGW 1 cut(s) 1260
PdmI GAANNNNTTC 1 cut(s) 1152
PfeI GAWTC 5 cut(s) 200, 230, 388, 1018, 1159
PkrI GCNGC 7 cut(s) 58, 82, 108, 607, 792, 809, 1121
Ple19I CGATCG 1 cut(s) 888
PleI GAGTC 1 cut(s) 1376
PpsI GAGTC 1 cut(s) 1376
Psp124BI GAGCTC 1 cut(s) 1263
Psp1406I AACGTT 1 cut(s) 1177
PspPI GGNCC 1 cut(s) 48
PvuI CGATCG 1 cut(s) 888
RsaI GTAC 4 cut(s) 323, 586, 638, 700
RsaNI GTAC 4 cut(s) 322, 585, 637, 699
RseI CAYNNNNRTG 3 cut(s) 156, 354, 930
SacI GAGCTC 1 cut(s) 1263
SatI GCNGC 7 cut(s) 57, 81, 107, 606, 791, 808, 1120
Sau3AI GATC 5 cut(s) 598, 763, 819, 885, 1105
Sau96I GGNCC 1 cut(s) 48
SchI GAGTC 1 cut(s) 1376
SduI GDGCHC 2 cut(s) 635, 1263
SfaNI GCATC 5 cut(s) 102, 456, 952, 1106, 1327
SfcI CTRYAG 1 cut(s) 651
Sfr274I CTCGAG 1 cut(s) 169
SinI GGWCC 1 cut(s) 48
SlaI CTCGAG 1 cut(s) 169
SmiMI CAYNNNNRTG 3 cut(s) 156, 354, 930
SmlI CTYRAG 1 cut(s) 169
SmoI CTYRAG 1 cut(s) 169
SpeI ACTAGT 1 cut(s) 917
Sse9I AATT 6 cut(s) 359, 559, 674, 986, 1547, 1560
SspI AATATT 1 cut(s) 538
SspMI CTAG 5 cut(s) 197, 918, 1022, 1322, 1582
SstI GAGCTC 1 cut(s) 1263
TaaI ACNGT 3 cut(s) 251, 296, 1226
TaiI ACGT 2 cut(s) 1153, 1180
TaqI TCGA 7 cut(s) 170, 614, 726, 1197, 1263, 1380, 1528
TaqII GACCGA 1 cut(s) 672
TasI AATT 6 cut(s) 359, 559, 674, 986, 1547, 1560
TauI GCSGC 4 cut(s) 59, 83, 109, 608
TfiI GAWTC 5 cut(s) 200, 230, 388, 1018, 1159
TscAI CASTG 1 cut(s) 1058
TseFI GTSAC 2 cut(s) 96, 841
TseI GCWGC 3 cut(s) 790, 807, 1119
Tsp45I GTSAC 2 cut(s) 96, 841
TspDTI ATGAA 6 cut(s) 119, 230, 372, 591, 914, 1290
TspGWI ACGGA 1 cut(s) 1128
TspRI CASTG 1 cut(s) 1058
VpaK11BI GGWCC 1 cut(s) 48
XapI RAATTY 2 cut(s) 1547, 1560
XceI RCATGY 3 cut(s) 1029, 1039, 1205
XcmI CCANNNNNNNNNTGG 1 cut(s) 158
XhoI CTCGAG 1 cut(s) 169
XmnI GAANNNNTTC 1 cut(s) 1152
XspI CTAG 5 cut(s) 197, 918, 1022, 1322, 1582
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.