AT5G43110

Encodes a member of the Arabidopsis Pumilio (APUM) proteins containing PUF domain (eight repeats of approximately 36 amino acids each). PUF proteins regulate both mRNA stability and translation through sequence-specific binding to the 3' UTR of target mRNA transcripts

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0004567
Physical Location & Seq
Forward (+)
17309842 .. 17311937
2096 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G43110.1

Sequence Viewer

Length: 1557 bp
ATGGACAAGAATTTTCGTGTCAACACTAACGACGAACGTAACATCTGGTTGTCCACGGCGGTGAATGAGAATCTGACAATGGCGTCGTCGTCATCTCAGCCACAACCAATATCGTCTCCGTTTCATCAACCAGAGAATCAGGTCAACAGAGTTAATCCTGGAAGTCATTATTATGATTTGGAAACCCTAGAATCTTCTTTTGGTGGGCTTTCTTTCAACGATTCAAGTGTTGGTCAGAATGGTGATTCGATTCACCTCCCTCGTCGAACCAATCAGGTATTCACTGGCAGTAGTTCTGGTGGTGCTGGTGATGACAATGGCTACTTGTTACCTCCGATGGGTTCTCATCACCACCGTGAATTAGAGGAGTTGCAAAGGCATAATTATCTGAATCAACTGAGAATGTCGTATCAGAACGATTATGCTCATCAATCTTATTGGTATAATACTGATGGTGATGGTAATGGGATGCTTAATAATGGTTTCTTGAATGATGTTCCTTCTTCTTCAAGAGACCGTGTCTCAGATTACTACACTAATCGTTTTGGGTATGAAGGTTACAACTATTGGAGGGGTAATGAAGGTTTTGACTATAATCAATGTCAGGCCTCTTTCTCTGCCTTTGCTAAAGACAAAGAAATGAGTGAGAGGTTGGGGATGAGCATCTTTCAGGGTACTAAAGAGACAGTTGATGCGATTTACAACGGTCTCATTGGCGATATTTGTGAGTTGATGGTGGATCCTTATGGTAGTGATGTTGTTCAGTTGCTTATGAGAAGATGCAGCTCTGAACAAATCGTTCAGCTCGTTGATATTGTCACTCAGCAAATGTTCCAGTTTGTTAACATATGCATTGATTCTCTCGGAACCAATGCTATACAAGTTCTTTTGACATGTATTAATGAGCGTGCTAAAGATCAAATCCCGCGCATCGTGGATGTTGTAAGAACGGTAGCACTTCAATTGTCAAAGAGCAACCATGCCATCTTTGTGATTCTCGCATGCTTCCGTCTATTTCCTTTGCACTGTAGGTTACTTCTTGAACTTATCGTTCAAAATTGCCACCAGATAGCCATTGATCAACACGGGTGCTGCCTGCTTCAGCTATGTTTTAACAAGGACCGGGTTCCAAATCTAGAGATAAGGCAACGTTTGATTATGGAAGCAATCGCTAATGCATTGAGACTCTGCTTAAACTGCTACGGAAACTATGTGGTTCAGTATATCGTGGAGCTCAATAATCGTTATTTAATAGACGCGCTCGTGAGACAGCTAATAGGGAACTATGCACACCTTGCCCGCAACAAATATGGAAGTCATGCTGTGCAGAAGTTGTTGAAACTAAGGTGGATCGACTCTAGGGTTATTGTAATTGACTTGCTTAGAGAGATTGATACACTTCTTCTTGACCCGTTTGGAAACTATGTCATTCAAACCGCATGGTTTGTGTCTAAGGACGATGTGCGCCGGATGTTGAGGTATCATATAGAGCGTAACATTCCAATGATGAGGTGCAACAAATTTGGGAACAAAGTTCTGGAGAAACTCAATATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUF

Protein Analysis

518

Amino Acids

59.45

Weight (kDa)

6.21

Isoelectric Point (pI)

38.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUF PF00806 241 - 273 8e-06 Pumilio-family RNA binding repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 82
AccII CGCG 2 cut(s) 928, 1259
AciI CCGC 4 cut(s) 59, 926, 1300, 1437
AclI AACGTT 1 cut(s) 1150
AclWI GGATC 3 cut(s) 734, 747, 1358
AcsI RAATTY 2 cut(s) 10, 1520
AcuI CTGAAG 1 cut(s) 1085
AcyI GRCGYC 1 cut(s) 83
AfaI GTAC 1 cut(s) 676
AfiI CCNNNNNNNGG 1 cut(s) 338
AflIII ACRYGT 1 cut(s) 893
AgsI TTSAA 9 cut(s) 217, 225, 490, 510, 962, 1043, 1055, 1339, 1433
AjnI CCWGG 1 cut(s) 157
AjuI GAANNNNNNNTTGG 4 cut(s) 183, 215, 263, 295
AleI CACNNNNGTG 2 cut(s) 59, 354
AluBI AGCT 5 cut(s) 786, 805, 1105, 1234, 1273
AluI AGCT 5 cut(s) 786, 805, 1105, 1234, 1273
Alw21I GWGCWC 1 cut(s) 1236
Alw26I GTCTC 7 cut(s) 120, 507, 526, 677, 713, 1177, 1261
AlwI GGATC 3 cut(s) 734, 747, 1358
AoxI GGCC 1 cut(s) 606
ApeKI GCWGC 2 cut(s) 783, 1092
ApoI RAATTY 2 cut(s) 10, 1520
AseI ATTAAT 1 cut(s) 900
AspLEI GCGC 3 cut(s) 930, 1261, 1467
AspS9I GGNCC 1 cut(s) 1120
AsuC2I CCSGG 1 cut(s) 1124
AsuHPI GGTGA 6 cut(s) 73, 245, 254, 320, 341, 467
AvaII GGWCC 1 cut(s) 1120
BamHI GGATCC 1 cut(s) 739
BanII GRGCYC 1 cut(s) 1236
BauI CACGAG 1 cut(s) 1262
Bbv12I GWGCWC 1 cut(s) 1236
BbvI GCAGC 2 cut(s) 795, 1079
BccI CCATC 5 cut(s) 331, 446, 452, 727, 992
BceAI ACGGC 1 cut(s) 72
BciT130I CCWGG 1 cut(s) 159
BclI TGATCA 1 cut(s) 1078
BcnI CCSGG 1 cut(s) 1124
BcoDI GTCTC 7 cut(s) 120, 507, 526, 677, 713, 1177, 1261
BfaI CTAG 4 cut(s) 188, 1136, 1359, 1555
BfmI CTRYAG 1 cut(s) 1027
BisI GCNGC 2 cut(s) 784, 1093
BlsI GCNGC 2 cut(s) 785, 1094
Bme1390I CCNGG 2 cut(s) 159, 1124
Bme18I GGWCC 1 cut(s) 1120
BmgT120I GGNCC 1 cut(s) 1120
BmiI GGNNCC 3 cut(s) 741, 868, 1128
BmrFI CCNGG 2 cut(s) 159, 1124
BmsI GCATC 5 cut(s) 459, 672, 682, 770, 939
BpuMI CCSGG 1 cut(s) 1124
BsaBI GATNNNNATC 1 cut(s) 662
BsaHI GRCGYC 1 cut(s) 83
BsaI GGTCTC 2 cut(s) 507, 713
BsaJI CCNNGG 1 cut(s) 54
Bsc4I CCNNNNNNNGG 1 cut(s) 338
Bse1I ACTGG 2 cut(s) 289, 835
Bse8I GATNNNNATC 1 cut(s) 662
BseBI CCWGG 1 cut(s) 159
BseDI CCNNGG 1 cut(s) 54
BseGI GGATG 4 cut(s) 474, 663, 943, 1476
BseJI GATNNNNATC 1 cut(s) 662
BseLI CCNNNNNNNGG 1 cut(s) 338
BseMII CTCAG 4 cut(s) 110, 389, 537, 836
BseNI ACTGG 2 cut(s) 289, 835
BseRI GAGGAG 1 cut(s) 380
BseXI GCAGC 2 cut(s) 795, 1079
BsgI GTGCAG 1 cut(s) 1346
Bsh1236I CGCG 2 cut(s) 928, 1259
BshFI GGCC 1 cut(s) 608
BsiHKAI GWGCWC 1 cut(s) 1236
BsiSI CCGG 2 cut(s) 1123, 1468
BslI CCNNNNNNNGG 1 cut(s) 338
BsmAI GTCTC 7 cut(s) 120, 507, 526, 677, 713, 1177, 1261
BsmBI CGTCTC 1 cut(s) 120
BsnI GGCC 1 cut(s) 608
Bso31I GGTCTC 2 cut(s) 507, 713
Bsp1286I GDGCHC 1 cut(s) 1236
Bsp143I GATC 4 cut(s) 739, 916, 1078, 1350
BspACI CCGC 4 cut(s) 59, 926, 1300, 1437
BspANI GGCC 1 cut(s) 608
BspCNI CTCAG 4 cut(s) 109, 390, 536, 835
BspFNI CGCG 2 cut(s) 928, 1259
BspLI GGNNCC 3 cut(s) 741, 868, 1128
BspPI GGATC 3 cut(s) 734, 747, 1358
BspTNI GGTCTC 2 cut(s) 507, 713
BsrI ACTGG 2 cut(s) 289, 835
BssECI CCNNGG 1 cut(s) 54
BssMI GATC 4 cut(s) 739, 916, 1078, 1350
BssNI GRCGYC 1 cut(s) 83
BssSI CACGAG 1 cut(s) 1262
Bst2BI CACGAG 1 cut(s) 1262
Bst2UI CCWGG 1 cut(s) 159
Bst4CI ACNGT 6 cut(s) 356, 518, 688, 707, 952, 1028
BstACI GRCGYC 1 cut(s) 83
BstAPI GCANNNNNTGC 1 cut(s) 1295
BstC8I GCNNGC 4 cut(s) 909, 1003, 1097, 1300
BstDEI CTNAG 7 cut(s) 96, 398, 523, 822, 1343, 1382, 1452
BstDSI CCRYGG 1 cut(s) 54
BstF5I GGATG 4 cut(s) 474, 663, 943, 1476
BstFNI CGCG 2 cut(s) 928, 1259
BstHHI GCGC 3 cut(s) 930, 1261, 1467
BstKTI GATC 4 cut(s) 742, 919, 1081, 1353
BstMAI GTCTC 7 cut(s) 120, 507, 526, 677, 713, 1177, 1261
BstMBI GATC 4 cut(s) 739, 916, 1078, 1350
BstMWI GCNNNNNNNGC 2 cut(s) 1197, 1295
BstNI CCWGG 1 cut(s) 159
BstNSI RCATGY 2 cut(s) 897, 1005
BstSCI CCNGG 2 cut(s) 157, 1122
BstSFI CTRYAG 1 cut(s) 1027
BstUI CGCG 2 cut(s) 928, 1259
BstV1I GCAGC 2 cut(s) 795, 1079
BstX2I RGATCY 1 cut(s) 739
BstYI RGATCY 1 cut(s) 739
BsuRI GGCC 1 cut(s) 608
BtgI CCRYGG 1 cut(s) 54
BtsCI GGATG 4 cut(s) 474, 663, 943, 1476
BtsIMutI CAGTG 2 cut(s) 282, 1024
Cac8I GCNNGC 4 cut(s) 909, 1003, 1097, 1300
CfoI GCGC 3 cut(s) 930, 1261, 1467
Cfr13I GGNCC 1 cut(s) 1120
CseI GACGC 2 cut(s) 72, 1265
Csp6I GTAC 1 cut(s) 675
CviAII CATG 5 cut(s) 894, 980, 1002, 1319, 1440
CviQI GTAC 1 cut(s) 675
DdeI CTNAG 7 cut(s) 96, 398, 523, 822, 1343, 1382, 1452
DpnI GATC 4 cut(s) 741, 918, 1080, 1352
DpnII GATC 4 cut(s) 739, 916, 1078, 1350
DrdI GACNNNNNNGTC 1 cut(s) 82
DseDI GACNNNNNNGTC 1 cut(s) 82
Ecl136II GAGCTC 1 cut(s) 1234
Eco147I AGGCCT 1 cut(s) 608
Eco24I GRGCYC 1 cut(s) 1236
Eco31I GGTCTC 2 cut(s) 507, 713
Eco47I GGWCC 1 cut(s) 1120
Eco53kI GAGCTC 1 cut(s) 1234
Eco57I CTGAAG 1 cut(s) 1085
EcoICRI GAGCTC 1 cut(s) 1234
EcoRII CCWGG 1 cut(s) 157
EcoT22I ATGCAT 2 cut(s) 854, 1180
EcoT38I GRGCYC 1 cut(s) 1236
Esp3I CGTCTC 1 cut(s) 120
FaeI CATG 5 cut(s) 897, 983, 1005, 1322, 1443
FatI CATG 5 cut(s) 893, 979, 1001, 1318, 1439
FauI CCCGC 2 cut(s) 933, 1307
FauNDI CATATG 1 cut(s) 848
FbaI TGATCA 1 cut(s) 1078
Fnu4HI GCNGC 2 cut(s) 784, 1093
FokI GGATG 4 cut(s) 481, 670, 950, 1483
FriOI GRGCYC 1 cut(s) 1236
Fsp4HI GCNGC 2 cut(s) 784, 1093
FspBI CTAG 4 cut(s) 188, 1136, 1359, 1555
GlaI GCGC 3 cut(s) 929, 1260, 1466
GluI GCNGC 2 cut(s) 784, 1093
HaeIII GGCC 1 cut(s) 608
HapII CCGG 2 cut(s) 1123, 1468
HgaI GACGC 2 cut(s) 72, 1265
HhaI GCGC 3 cut(s) 930, 1261, 1467
Hin1I GRCGYC 1 cut(s) 83
Hin1II CATG 5 cut(s) 897, 983, 1005, 1322, 1443
Hin6I GCGC 3 cut(s) 928, 1259, 1465
HinP1I GCGC 3 cut(s) 928, 1259, 1465
HincII GTYRAC 3 cut(s) 22, 145, 844
HindII GTYRAC 3 cut(s) 22, 145, 844
HpaI GTTAAC 1 cut(s) 844
HpaII CCGG 2 cut(s) 1123, 1468
HphI GGTGA 6 cut(s) 73, 245, 254, 320, 341, 467
Hpy166II GTNNAC 4 cut(s) 22, 54, 145, 844
Hpy188I TCNGA 8 cut(s) 75, 237, 336, 390, 414, 526, 790, 866
Hpy188III TCNNGA 7 cut(s) 487, 510, 1040, 1136, 1264, 1406, 1538
Hpy8I GTNNAC 4 cut(s) 22, 54, 145, 844
Hpy99I CGWCG 4 cut(s) 35, 88, 91, 267
HpyAV CCTTC 3 cut(s) 510, 548, 575
HpyCH4III ACNGT 6 cut(s) 356, 518, 688, 707, 952, 1028
HpyCH4IV ACGT 2 cut(s) 37, 1150
HpyCH4V TGCA 8 cut(s) 373, 783, 852, 1024, 1178, 1289, 1327, 1515
HpyF10VI GCNNNNNNNGC 2 cut(s) 1197, 1295
HpyF3I CTNAG 7 cut(s) 96, 398, 523, 822, 1343, 1382, 1452
HpySE526I ACGT 2 cut(s) 37, 1150
Hsp92I GRCGYC 1 cut(s) 83
Hsp92II CATG 5 cut(s) 897, 983, 1005, 1322, 1443
HspAI GCGC 3 cut(s) 928, 1259, 1465
Ksp22I TGATCA 1 cut(s) 1078
KspAI GTTAAC 1 cut(s) 844
Kzo9I GATC 4 cut(s) 739, 916, 1078, 1350
LmnI GCTCC 1 cut(s) 1231
Lsp1109I GCAGC 2 cut(s) 795, 1079
LweI GCATC 5 cut(s) 459, 672, 682, 770, 939
MaeI CTAG 4 cut(s) 188, 1136, 1359, 1555
MaeII ACGT 2 cut(s) 37, 1150
MaeIII GTNAC 6 cut(s) 38, 327, 557, 817, 1032, 1493
MalI GATC 4 cut(s) 741, 918, 1080, 1352
MboI GATC 4 cut(s) 739, 916, 1078, 1350
MboII GAAGA 5 cut(s) 186, 495, 498, 789, 1394
MfeI CAATTG 1 cut(s) 962
MflI RGATCY 1 cut(s) 739
MhlI GDGCHC 1 cut(s) 1236
MluCI AATT 7 cut(s) 10, 359, 382, 962, 1057, 1371, 1520
MlyI GAGTC 2 cut(s) 1179, 1349
MnlI CCTC 9 cut(s) 266, 270, 342, 358, 564, 619, 642, 1470, 1503
Mph1103I ATGCAT 2 cut(s) 854, 1180
MseI TTAA 7 cut(s) 153, 474, 843, 900, 1113, 1193, 1250
MslI CAYNNNNRTG 5 cut(s) 59, 171, 354, 989, 1502
MspI CCGG 2 cut(s) 1123, 1468
MspR9I CCNGG 2 cut(s) 159, 1124
MunI CAATTG 1 cut(s) 962
MvaI CCWGG 1 cut(s) 159
MvnI CGCG 2 cut(s) 928, 1259
MwoI GCNNNNNNNGC 2 cut(s) 1197, 1295
NciI CCSGG 1 cut(s) 1124
NdeI CATATG 1 cut(s) 848
NdeII GATC 4 cut(s) 739, 916, 1078, 1350
NlaIII CATG 5 cut(s) 897, 983, 1005, 1322, 1443
NlaIV GGNNCC 3 cut(s) 741, 868, 1128
NmuCI GTSAC 1 cut(s) 817
NsiI ATGCAT 2 cut(s) 854, 1180
NspI RCATGY 2 cut(s) 897, 1005
OliI CACNNNNGTG 2 cut(s) 59, 354
PaeI GCATGC 1 cut(s) 1005
PceI AGGCCT 1 cut(s) 608
PciI ACATGT 1 cut(s) 893
PcsI WCGNNNNNNNCGW 1 cut(s) 804
PfeI GAWTC 9 cut(s) 70, 136, 191, 221, 245, 250, 391, 857, 994
PflFI GACNNNGTC 1 cut(s) 518
PfoI TCCNGGA 1 cut(s) 157
PkrI GCNGC 2 cut(s) 785, 1094
PleI GAGTC 2 cut(s) 1179, 1349
PpsI GAGTC 2 cut(s) 1179, 1349
PscI ACATGT 1 cut(s) 893
PshBI ATTAAT 1 cut(s) 900
Psp124BI GAGCTC 1 cut(s) 1236
Psp1406I AACGTT 1 cut(s) 1150
Psp6I CCWGG 1 cut(s) 157
PspGI CCWGG 1 cut(s) 157
PspN4I GGNNCC 3 cut(s) 741, 868, 1128
PspPI GGNCC 1 cut(s) 1120
PsuI RGATCY 1 cut(s) 739
PsyI GACNNNGTC 1 cut(s) 518
RsaI GTAC 1 cut(s) 676
RsaNI GTAC 1 cut(s) 675
RseI CAYNNNNRTG 5 cut(s) 59, 171, 354, 989, 1502
SacI GAGCTC 1 cut(s) 1236
SaqAI TTAA 7 cut(s) 153, 474, 843, 900, 1113, 1193, 1250
SatI GCNGC 2 cut(s) 784, 1093
Sau3AI GATC 4 cut(s) 739, 916, 1078, 1350
Sau96I GGNCC 1 cut(s) 1120
SchI GAGTC 2 cut(s) 1179, 1349
ScrFI CCNGG 2 cut(s) 159, 1124
SduI GDGCHC 1 cut(s) 1236
SfaNI GCATC 5 cut(s) 459, 672, 682, 770, 939
SfcI CTRYAG 1 cut(s) 1027
SinI GGWCC 1 cut(s) 1120
SmiMI CAYNNNNRTG 5 cut(s) 59, 171, 354, 989, 1502
SphI GCATGC 1 cut(s) 1005
Sse9I AATT 7 cut(s) 10, 359, 382, 962, 1057, 1371, 1520
SseBI AGGCCT 1 cut(s) 608
SsiI CCGC 4 cut(s) 59, 926, 1300, 1437
SspMI CTAG 4 cut(s) 188, 1136, 1359, 1555
SstI GAGCTC 1 cut(s) 1236
StuI AGGCCT 1 cut(s) 608
StyD4I CCNGG 2 cut(s) 157, 1122
TaaI ACNGT 6 cut(s) 356, 518, 688, 707, 952, 1028
TaiI ACGT 2 cut(s) 40, 1153
TaqI TCGA 3 cut(s) 248, 265, 1353
TasI AATT 7 cut(s) 10, 359, 382, 962, 1057, 1371, 1520
TfiI GAWTC 9 cut(s) 70, 136, 191, 221, 245, 250, 391, 857, 994
Tru1I TTAA 7 cut(s) 153, 474, 843, 900, 1113, 1193, 1250
Tru9I TTAA 7 cut(s) 153, 474, 843, 900, 1113, 1193, 1250
TscAI CASTG 2 cut(s) 289, 1031
TseFI GTSAC 1 cut(s) 817
TseI GCWGC 2 cut(s) 783, 1092
Tsp45I GTSAC 1 cut(s) 817
TspDTI ATGAA 3 cut(s) 113, 567, 594
TspGWI ACGGA 3 cut(s) 108, 998, 1218
TspRI CASTG 2 cut(s) 289, 1031
Tth111I GACNNNGTC 1 cut(s) 518
VpaK11BI GGWCC 1 cut(s) 1120
VspI ATTAAT 1 cut(s) 900
XapI RAATTY 2 cut(s) 10, 1520
XbaI TCTAGA 1 cut(s) 1135
XceI RCATGY 2 cut(s) 897, 1005
XspI CTAG 4 cut(s) 188, 1136, 1359, 1555
Zsp2I ATGCAT 2 cut(s) 854, 1180
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.