AT5G55820

Inner centromere protein, ARK binding region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0002394
Physical Location & Seq
Forward (+)
22586287 .. 22594135
7849 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G55820.8

Sequence Viewer

Length: 5481 bp
ATGATCAGGGACAGCGAAAATAAGACGACGACGGCGAAAGTATTAAAAGATAGAGAGAAACGACATCGTATGAAAAAGAGTAGGTCGTCGGCGATGTTTTCCGTCAAGGAGAATCCGAGGGGGAAGACGGCGAATGTGAAGATTGAGAATCTTTTCGTTCAGATCTTTGAGAGGAAGAGGCGAATCGTCGAGCAGGTTCAGCAACAAGTAGATCTCTATGACCAGCATTTAGCTTCCAAATGCCTACTCGCCGGAGTATCTCCTCCGTCGTGGCTCTGGTCTCCGTCTCTACCTTCCCAAACTTCCGAGTTAAATAAGGAGGAGATTATATCAGAACTTCTATTTCCTTCATCAAGACCTTCCATCGTTTGTCCTAGCAGTCGTCCCTTTTCATACCAACGGCCTGTCAGGTTTCTAGCTGACAATGTAGTAAGACAAGACCTGACCTCTGTGGTAAATAACCCGCTAGAAGAGCAGTTGCTTGAAGAGGAACCGCAACACAACCTCTCACACAACTTAGTCAGACAAGTTTCGAATCATTCTCATGAGCAGGATGTTAATATTGCATCTCCTAGAGATGTACATGAGAAAGAGAGATTGCCAGAAAGTGTCTCAATCGATTGCAGAGAGAATCAAAGTTGTTCATCTCCCGAACACTCCAAGAATCAGAGAGTTGAAACTAATCTTGATGCTACATCTCCTGGATGTAGCCAAGGGGAAAAGGTTCCCAAATGTGTCTCAACTACTGGTTGTAAGCGGAAATCTTCATCTCTTGGTTATTGTCAAGAGGAAATTGAACCAGACACTTGCATTGACCCTGGATTATCACTTGCTAAGATGCAGAGATCAAGGTCACGTCAAAAAGCTTTGGAGCTTCGTAGTAGTGCAAAAGCGTCAAAAAGCCGTTCAAACAGTAGAAATGAGCTCAAACCTTCTCCGGGTGGTGATATAGGCTTTGGGATTGCTTCATTAAGGTCTGATAGTGTTAGTGAGATAAAGTTATTTAAGCATGATGAAAATGATGAAGAGTGTCGAGAAGAAGTGGAGAACAGTAATTCTCAAGGTAAAAGAGGAGATCAATGTATTAAGATTAGTGTACCTACAGAGTCTTTTACCTTGCATCATGAAGTGGATTCAGTGTCAATATCTTCAAGTGGTGATGCTTATGCTTCTATTGTACCAGAATGTCTACTCGAGTCTGGTCATGTGAATGACATTGATATATTACAGTCCATTGAGACAATTGATGAAGCGTCTGGCAAAGTAGATGAGCAAGTGGATGATCCCAAAAGCAGAAGTTGCTATGAAACAGCTTATCTCGATGGAAGTACAAGATCTAAAAGCTCAATTCAAGATAACTCCAAGAGGAAACATCAAAAATCAAGCAACTCCTTTTCTGGTAATTTTCTGTTAACAAATTCAAATCCCTCTCACTGGGCTGATCATGAAGTAGAATTACCTCAAGCAATAACTACGACTAGTGAAGTTTCTATGGTGACAGATGCGGGAACGAGCATCTTTCAGTCTGAAATCATTGCAAGATCTAGAAGTAATGCTCGAGAAAATAGATCCAAGACCGAGCATTCAGGCTCTGTTGAGTCTTCTTCAATTAACTTGGAGCCAAGAGATTCAATTCCAGTACTGCAAGGTAGCCATGTAAAAGATTCACTGAATCCCTCTAGTGTTGATGCTGAAGGTTTAGTAGTTGAAAATATTACTAGCAGCGATCAATCAAAAGAAACGGGTGAATGTGTTGACACTAACAGATGTTCAAGTGCTGAAAGGGTAAGCCAAACTGGTATCTCCCCAGATGAGACCACATTTGCGGGTGCAATCCAAGACTCTATATCCCAGATCGAGCTTTTGAGCTTTGTTGAGTCCTCTTCAATTGAACTGCAGTCGAGACACTCAGTCAAGCAATCAGACGATGAAAGTGTATTGTTGAAGCCCGTTACTGTTAATGGCGAAGCTTTATTAGTGGAGGAAGATAACAATGGTGAGTCAACTGAAATTAGCGGTATTTCAAAATCTAGAAGTTTAAGCCAAACTGACATCACGGTAGTTTTGCCAGTGGTGGTGGAATCTATTCTTAATGAAAGTGGTACTCCGGAAAAATTGATTGACCATTCTAAAAGATGTGATATCAGTTGTGGGTCCAAGGAAGTACAGCCACTGGGTTCATTGACCGAAGTGGGGAGTAACCAAAGCCATGGAATAATTAGTAGGGCAAGAAGCTCACTCATAGAAGAGGAATCAGCAAATGACTATAAGGCTCTTTCTGATGGGTCTAATCATAAATCGGCTGACAAACAACTTGAAGTTAGAGAAGGAAATTCATTGCTGAGAACCCCTGATCGCCCTGTTTTTGTGGACAACTTCGATGAGGTTCCAGAGAATAGTCGAGAAAAATCAAGCATGGAGAAGGTCCCCACCCCAGCACCCACCGCAAGGGTGTTTGATGTCCCATCTCTCACTGATTCTGGAGTAAATTTATCGGCAAACAATGAAATGAATGACATTGAAGATCACAATGGGTTAAACATAGAAATGGTAGCAGAAATGGAATCGTATGCAAGCCACCCTGGCTTAAAAGTGGGAGAGAATGAACCTACAGAGTCAAATACATTCACTGGCCATATAGATGCATTGACAAAGAGACCTCAACATGAAACATCCTCTGAAAAAGCTGTTCCCCCAATTAAAAGAGATGTAACATGTACAGAAGCAGATGAATGTCATGATCTAGAGAGCCCGATTCAAGAATTTTTCTGCTCTAGTTCCCCCATGGGGGGTTCCATGCGGCAGAATAAGCGGAGAAGAATCCTGGAAAAACCAACTAGAAGAGAGCTTTCGTCAAGTCCAGGGGGAGACATTCTCGAGTCAGATTATGTTAGGGAAGCAGTACATCATAGGGAGGAAGCCGCATGTCACAACGTCGATAACTATGACGTTGAGTTACAGAAGTTGATTGGATCTGCATCTTCACATCACTATAGTGTTGAGTTACAAAAAATGATTGGATCTGCATCGTCAGCTGAGTTACGATTTGAAGAGGGAGACATTCTCGAGTCAGATTATGTTAGAGAAGCAGTACATCATAGGGAGGAAGCCGCATGTCACAACGTCGATAACTATGACGTTGAGTTACAGAAGTTGATTGGATCTGCATCTTCACATCACTATAGTGTTGAGTTACAAAAAATGATTGGATCTGCATCGTCAGCTGAGTTACGATTTGAAGAGAGTTATTTACTCAAGGAAGCTGGATTGATGAGTCCTGCCTCGCTTTCCTACAGAACAGAACAGCTAAGTGTACAGAGGAGTCAAATTGCTCCAGATCACAGAGTTGGATCAGAAAATATTAACTTTTTTCCATATGCTGGTGAAACCTCACATGGATTAGCTAGTTGTATTGTTCGCGACTCAGATAGTTCTCCTTGCTTAACACCCTTGGGTTTGATAAGCTCAGACGATGGAAGCCCCCCTGTCTTGGAGGGTTTTATTATCCAGACTGATGATGAAAATCAAAGCGGCTCCAAAAACCAGTTAAATCATGACAGCTTCCAACTTCCAAGAACTACAGCAGAAAGTGCAGCCATGATAGAGCAGATTTGCAAGTCTGCTTGCATGAACACTCCGTCATTACATCTGGCTAAAACATTTAAGTTCGATGAAAAACTAGACTTGGATCAGTCTGTTTCAACCGAGCTGTTTGATGGCATGTTTTTCAGTCAGAATCTCGAGGGTAGCTCTGTCTTTGATAACTTGGGGATTAACCATGATTATACAGGAAGATCGTACACTGACTCTTTGCCTGGTACTGGCTCATCTGCTGAGGCTAGGAATCCTTGCATGTCACCAACTGAGAAGCTGTGGTATAGAAGTTTGCAAAAGTCTTCCAGTTCAGAGAAACGAAGCACTCAGACACCAGACCTACCTTGCATTAGCGAAGAGAATGAGAACATAGAAGAGGAAGCTGAGAACTTATGTACGAACACTCCAAAGTCTATGAGGTCAGAGAAGCGAGGAAGTTCAATTCCGGAACTTCCTTGCATAGCTGAAGAGAACGAAAACATAGATGAGATATCTGATGCTGTCAATGAAGCATCTGGTTCTGAAAGGGAGAATGTGTCTGCTGAAAGGAAACCTCTTGGTGATGTTAATGAAGATCCTATGAAGCTTCTTCCATCTGTTTCTGAAGCCAAGATTCCTGCCGATAGACAGAGTCTAGACTCTGTCAGTACTGCATTCAGCTTTTCAGCTAAGTGCAACAGTGTCAAAAGTAAAGTGGGAAAGCTGAGTAACCGAAGATTCACGGGTAAAGGTAAAGAGAACCAAGGTGGAGCAGGTGCTAAAAGAAATGTTAAACCGCCTAGTAGCAGGTTCAGTAAGCCTAAGTTGTCTTGCAACTCGAGTTTGACAACTGTAGGTCCACGGTTACAAGAAAAAGAACCTAGGCACAACAACATTGTCTCAAACATCACTTCGTTCGTTCCACTAGTGCAGCAGCAAAAACCAGCACCTGCACTAATTACAGGGAAAAGGGATGTCAAAGTAAAGGCCCTGGAGGCTGCTGAGGCTTCAAAACGTATTGCTGAACAGAAAGAGAATGATCGTAAGCTGAAGAAGGAAGCTATGAAGCTTGAACGGGCAAAACAGGAACAGGAAAATCTGAAAAAGCAAGAGATAGAGAAGAAAAAGAAAGAAGAAGATCGAAAGAAAAAGGAGGCAGAAATGGCTTGGAAGCAGGAGATGGAAAAGAAAAAGAAAGAAGAAGAAAGGAAGAGAAAGGAGTTTGAAATGGCTGATAGGAAAAGGCAGAGGGAAGAAGAAGACAAAAGGTTGAAGGAAGCTAAAAAAAGACAACGCATTGCAGATTTTCAAAGACAACAAAGAGAGGCTGATGAAAAGCTTCAAGCTGAAAAAGAATTGAAAAGACAAGCTATGGATGCGAGAATAAAAGCACAAAAGGAACTCAAAGAAGACCAAAATAATGCTGAGAAAACCAGGCAAGCGAATTCTAGGATCCCAGCGGTGAGATCAAAGAGTAATTCTAGTGATGATACCAATGCTTCAAGAAGCTCTAGAGAAAATGATTTCAAGGTGATAAGCAATCCAGGGAACATGTCTGAAGAAGCCAACATGGGAATTGAAGAAATGGAAGAGTCGTACAACATCTCTCCATACAAATGCTCAGATGACGAAGATGAAGAGGAAGACGACAATGACGACATGTCCAACAAAAAATTCGCTCCTACTTGGGCCAGCAAGAGCAATGTACGGCTCGCTGTCATTTCCCAACAAAACATTGATCCCGATGTTACTTTTCCTGCAAAAAGCGCCTGTGATATAAGTAACGGTTCTGTTGATTTTCCTAGTGGAAAGAGCCAGGTGCTGAAAGCAAGAGATTTGTTCTTTTTCAATATAACCAAATTCTCACTACTTTTCAATGTCGACTTCGTCAAAATAACTTTTGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
INCENP_ARK-bind

Protein Analysis

1826

Amino Acids

203.02

Weight (kDa)

5.29

Isoelectric Point (pI)

66.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
INCENP_ARK-bind PF03941 1638 - 1692 5.4e-10 Inner centromere protein, ARK binding region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 4337, 4531
Acc36I ACCTGC 4 cut(s) 184, 4337, 4371, 4531
AccB7I CCANNNNNTGG 1 cut(s) 3380
AccI GTMKAC 2 cut(s) 1189, 5454
AccII CGCG 1 cut(s) 3420
AccIII TCCGGA 2 cut(s) 2107, 4039
AcoI YGGCCR 1 cut(s) 2632
AcsI RAATTY 7 cut(s) 1417, 2332, 2488, 2762, 5017, 5246, 5432
AcuI CTGAAG 5 cut(s) 1713, 4080, 4218, 4643, 5151
AflIII ACRYGT 3 cut(s) 2714, 5124, 5232
AhdI GACNNNNNGTC 2 cut(s) 1910, 5233
AhlI ACTAGT 2 cut(s) 1478, 4498
AjiI CACGTC 1 cut(s) 857
AjuI GAANNNNNNNTTGG 2 cut(s) 5231, 5263
AleI CACNNNNGTG 2 cut(s) 2994, 3183
Alw21I GWGCWC 1 cut(s) 927
AlwNI CAGNNNCTG 4 cut(s) 1592, 2173, 4523, 5395
Ama87I CYCGRG 6 cut(s) 1193, 1557, 2876, 3065, 3740, 4411
Aor13HI TCCGGA 2 cut(s) 2107, 4039
AoxI GGCC 4 cut(s) 401, 2632, 4560, 5262
ApeKI GCWGC 5 cut(s) 1722, 3593, 4504, 4507, 4571
ApoI RAATTY 7 cut(s) 1417, 2332, 2488, 2762, 5017, 5246, 5432
Asp700I GAANNNNTTC 4 cut(s) 152, 723, 2085, 4911
AspA2I CCTAGG 1 cut(s) 4454
AspLEI GCGC 1 cut(s) 5342
AspS9I GGNCC 5 cut(s) 2154, 2425, 4430, 4561, 5262
AsuC2I CCSGG 1 cut(s) 939
AsuII TTCGAA 1 cut(s) 533
AvaI CYCGRG 6 cut(s) 1193, 1557, 2876, 3065, 3740, 4411
AvaII GGWCC 3 cut(s) 2154, 2425, 4430
AvrII CCTAGG 1 cut(s) 4454
BalI TGGCCA 1 cut(s) 2634
BamHI GGATCC 1 cut(s) 5025
BanII GRGCYC 2 cut(s) 927, 2753
BarI GAAGNNNNNNTAC 2 cut(s) 1440, 1472
BbsI GAAGAC 6 cut(s) 131, 1593, 3888, 4839, 4989, 5223
Bbv12I GWGCWC 1 cut(s) 927
BbvCI CCTCAGC 2 cut(s) 3834, 4575
BbvI GCAGC 5 cut(s) 1734, 3605, 4516, 4519, 4558
BccI CCATC 8 cut(s) 371, 1316, 2276, 2473, 3467, 3710, 4195, 4747
BceAI ACGGC 5 cut(s) 48, 144, 416, 888, 5297
BclI TGATCA 2 cut(s) 3, 1441
BcnI CCSGG 1 cut(s) 939
BcuI ACTAGT 2 cut(s) 1478, 4498
BfmI CTRYAG 8 cut(s) 1101, 1895, 2610, 2992, 3181, 3292, 3579, 4425
BfoI RGCGCY 1 cut(s) 5343
BfuAI ACCTGC 4 cut(s) 184, 4337, 4371, 4531
BglI GCCNNNNNGGC 2 cut(s) 2583, 4568
BglII AGATCT 4 cut(s) 162, 211, 1334, 1541
BisI GCNGC 9 cut(s) 1723, 2801, 2922, 3111, 3532, 3594, 4505, 4508, 4572
BlnI CCTAGG 1 cut(s) 4454
BlsI GCNGC 9 cut(s) 1724, 2802, 2923, 3112, 3533, 3595, 4506, 4509, 4573
BmcAI AGTACT 2 cut(s) 1641, 4243
Bme18I GGWCC 3 cut(s) 2154, 2425, 4430
BmeRI GACNNNNNGTC 2 cut(s) 1910, 5233
BmeT110I CYCGRG 6 cut(s) 1193, 1557, 2876, 3065, 3740, 4411
BmgBI CACGTC 1 cut(s) 857
BmgT120I GGNCC 5 cut(s) 2154, 2425, 4430, 4561, 5262
BmiI GGNNCC 9 cut(s) 492, 726, 1620, 2155, 2388, 2427, 2794, 3535, 5027
BmrI ACTGGG 2 cut(s) 1444, 2183
BmuI ACTGGG 2 cut(s) 1444, 2183
BpiI GAAGAC 6 cut(s) 131, 1593, 3888, 4839, 4989, 5223
BplI GAGNNNNNCTC 6 cut(s) 2859, 2891, 3048, 3080, 3734, 3766
BpmI CTGGAG 3 cut(s) 2502, 3318, 4586
Bpu10I CCTNAGC 2 cut(s) 3834, 4575
Bpu14I TTCGAA 1 cut(s) 533
BpuEI CTTGAG 3 cut(s) 1044, 1446, 3239
BpuMI CCSGG 1 cut(s) 939
Bsa29I ATCGAT 1 cut(s) 618
BsaBI GATNNNNATC 5 cut(s) 2977, 3025, 3166, 3214, 3522
BsaI GGTCTC 3 cut(s) 285, 1808, 2650
BsaWI WCCGGW 2 cut(s) 2107, 4039
BsaXI ACNNNNNCTCC 4 cut(s) 3982, 4012, 4115, 4145
Bse3DI GCAATG 4 cut(s) 1533, 2336, 4869, 5281
Bse8I GATNNNNATC 5 cut(s) 2977, 3025, 3166, 3214, 3522
BseAI TCCGGA 2 cut(s) 2107, 4039
BseCI ATCGAT 1 cut(s) 618
BseGI GGATG 6 cut(s) 559, 710, 1285, 2672, 4552, 4954
BseJI GATNNNNATC 5 cut(s) 2977, 3025, 3166, 3214, 3522
BseMI GCAATG 4 cut(s) 1533, 2336, 4869, 5281
BseRI GAGGAG 4 cut(s) 252, 335, 1086, 3334
BseXI GCAGC 5 cut(s) 1734, 3605, 4516, 4519, 4558
BseYI CCCAGC 2 cut(s) 2434, 5029
BsgI GTGCAG 3 cut(s) 3612, 4509, 4523
Bsh1236I CGCG 1 cut(s) 3420
BshFI GGCC 4 cut(s) 403, 2634, 4562, 5264
BshVI ATCGAT 1 cut(s) 618
BsiHKAI GWGCWC 1 cut(s) 927
BsiHKCI CYCGRG 6 cut(s) 1193, 1557, 2876, 3065, 3740, 4411
BsiSI CCGG 4 cut(s) 252, 938, 2108, 4040
BslFI GGGAC 4 cut(s) 23, 369, 2411, 2447
BsmBI CGTCTC 1 cut(s) 291
BsmFI GGGAC 4 cut(s) 23, 369, 2411, 2447
BsmI GAATGC 2 cut(s) 1582, 4247
BsnI GGCC 4 cut(s) 403, 2634, 4562, 5264
Bso31I GGTCTC 3 cut(s) 285, 1808, 2650
BsoBI CYCGRG 6 cut(s) 1193, 1557, 2876, 3065, 3740, 4411
Bsp119I TTCGAA 1 cut(s) 533
Bsp1286I GDGCHC 2 cut(s) 927, 2753
Bsp13I TCCGGA 2 cut(s) 2107, 4039
Bsp1407I TGTACA 3 cut(s) 580, 2717, 3313
Bsp19I CCATGG 2 cut(s) 2209, 2784
Bsp68I TCGCGA 1 cut(s) 3420
BspANI GGCC 4 cut(s) 403, 2634, 4562, 5264
BspDI ATCGAT 1 cut(s) 618
BspEI TCCGGA 2 cut(s) 2107, 4039
BspFNI CGCG 1 cut(s) 3420
BspHI TCATGA 5 cut(s) 544, 1123, 1444, 2737, 3553
BspLI GGNNCC 9 cut(s) 492, 726, 1620, 2155, 2388, 2427, 2794, 3535, 5027
BspMAI CTGCAG 1 cut(s) 1899
BspMI ACCTGC 4 cut(s) 184, 4337, 4371, 4531
BspQI GCTCTTC 1 cut(s) 465
BspT104I TTCGAA 1 cut(s) 533
BspTNI GGTCTC 3 cut(s) 285, 1808, 2650
BsrDI GCAATG 4 cut(s) 1533, 2336, 4869, 5281
BsrGI TGTACA 3 cut(s) 580, 2717, 3313
BssT1I CCWWGG 7 cut(s) 712, 2157, 2209, 2784, 3450, 4336, 4454
Bst4CI ACNGT 9 cut(s) 914, 1052, 1230, 1957, 2059, 4274, 4426, 4437, 5360
BstAPI GCANNNNNTGC 2 cut(s) 1299, 3590
BstAUI TGTACA 3 cut(s) 580, 2717, 3313
BstBI TTCGAA 1 cut(s) 533
BstC8I GCNNGC 5 cut(s) 2575, 3625, 5013, 5266, 5286
BstDSI CCRYGG 3 cut(s) 2209, 2784, 4433
BstF5I GGATG 6 cut(s) 559, 710, 1285, 2672, 4552, 4954
BstFNI CGCG 1 cut(s) 3420
BstH2I RGCGCY 1 cut(s) 5343
BstHHI GCGC 1 cut(s) 5342
BstNSI RCATGY 7 cut(s) 2718, 2928, 3117, 3724, 3856, 5128, 5236
BstSFI CTRYAG 8 cut(s) 1101, 1895, 2610, 2992, 3181, 3292, 3579, 4425
BstUI CGCG 1 cut(s) 3420
BstV1I GCAGC 5 cut(s) 1734, 3605, 4516, 4519, 4558
BstV2I GAAGAC 6 cut(s) 131, 1593, 3888, 4839, 4989, 5223
BstXI CCANNNNNNTGG 1 cut(s) 2210
Bsu15I ATCGAT 1 cut(s) 618
BsuRI GGCC 4 cut(s) 403, 2634, 4562, 5264
BsuTUI ATCGAT 1 cut(s) 618
BtgI CCRYGG 3 cut(s) 2209, 2784, 4433
BtgZI GCGATG 1 cut(s) 107
BtrI CACGTC 1 cut(s) 857
BtsCI GGATG 6 cut(s) 559, 710, 1285, 2672, 4552, 4954
BtsIMutI CAGTG 9 cut(s) 1143, 1432, 1667, 2076, 2171, 2472, 2628, 3801, 4279
BtuMI TCGCGA 1 cut(s) 3420
BveI ACCTGC 4 cut(s) 184, 4337, 4371, 4531
Cac8I GCNNGC 5 cut(s) 2575, 3625, 5013, 5266, 5286
CaiI CAGNNNCTG 4 cut(s) 1592, 2173, 4523, 5395
CciI TCATGA 5 cut(s) 544, 1123, 1444, 2737, 3553
CfoI GCGC 1 cut(s) 5342
Cfr13I GGNCC 5 cut(s) 2154, 2425, 4430, 4561, 5262
ClaI ATCGAT 1 cut(s) 618
CseI GACGC 2 cut(s) 882, 1242
DriI GACNNNNNGTC 2 cut(s) 1910, 5233
EaeI YGGCCR 1 cut(s) 2632
Eam1105I GACNNNNNGTC 2 cut(s) 1910, 5233
Ecl136II GAGCTC 1 cut(s) 925
Eco130I CCWWGG 7 cut(s) 712, 2157, 2209, 2784, 3450, 4336, 4454
Eco24I GRGCYC 2 cut(s) 927, 2753
Eco31I GGTCTC 3 cut(s) 285, 1808, 2650
Eco32I GATATC 2 cut(s) 2143, 4086
Eco47I GGWCC 3 cut(s) 2154, 2425, 4430
Eco53kI GAGCTC 1 cut(s) 925
Eco57I CTGAAG 5 cut(s) 1713, 4080, 4218, 4643, 5151
Eco88I CYCGRG 6 cut(s) 1193, 1557, 2876, 3065, 3740, 4411
EcoICRI GAGCTC 1 cut(s) 925
EcoO109I RGGNCCY 2 cut(s) 2425, 4561
EcoRI GAATTC 1 cut(s) 5017
EcoRV GATATC 2 cut(s) 2143, 4086
EcoT14I CCWWGG 7 cut(s) 712, 2157, 2209, 2784, 3450, 4336, 4454
EcoT22I ATGCAT 1 cut(s) 2647
EcoT38I GRGCYC 2 cut(s) 927, 2753
ErhI CCWWGG 7 cut(s) 712, 2157, 2209, 2784, 3450, 4336, 4454
Esp3I CGTCTC 1 cut(s) 291
FaqI GGGAC 4 cut(s) 23, 369, 2411, 2447
FauI CCCGC 3 cut(s) 471, 1498, 1819
FauNDI CATATG 1 cut(s) 3376
FbaI TGATCA 2 cut(s) 3, 1441
FblI GTMKAC 2 cut(s) 1189, 5454
Fnu4HI GCNGC 9 cut(s) 1723, 2801, 2922, 3111, 3532, 3594, 4505, 4508, 4572
FokI GGATG 6 cut(s) 566, 717, 1292, 2659, 4559, 4961
FriOI GRGCYC 2 cut(s) 927, 2753
Fsp4HI GCNGC 9 cut(s) 1723, 2801, 2922, 3111, 3532, 3594, 4505, 4508, 4572
GlaI GCGC 1 cut(s) 5341
GluI GCNGC 9 cut(s) 1723, 2801, 2922, 3111, 3532, 3594, 4505, 4508, 4572
GsaI CCCAGC 2 cut(s) 2438, 5033
GsuI CTGGAG 3 cut(s) 2502, 3318, 4586
HaeII RGCGCY 1 cut(s) 5343
HaeIII GGCC 4 cut(s) 403, 2634, 4562, 5264
HapII CCGG 4 cut(s) 252, 938, 2108, 4040
HgaI GACGC 2 cut(s) 882, 1242
HhaI GCGC 1 cut(s) 5342
Hin6I GCGC 1 cut(s) 5340
HinP1I GCGC 1 cut(s) 5340
HincII GTYRAC 4 cut(s) 1413, 1756, 2004, 5455
HindII GTYRAC 4 cut(s) 1413, 1756, 2004, 5455
HindIII AAGCTT 5 cut(s) 864, 1968, 4178, 4640, 4910
HpaI GTTAAC 1 cut(s) 1413
HpaII CCGG 4 cut(s) 252, 938, 2108, 4040
Hpy99I CGWCG 7 cut(s) 31, 34, 91, 191, 271, 2939, 3128
HpyAV CCTTC 9 cut(s) 303, 357, 369, 942, 1688, 2321, 2416, 4621, 4840
HpyCH4III ACNGT 9 cut(s) 914, 1052, 1230, 1957, 2059, 4274, 4426, 4437, 5360
HpyCH4IV ACGT 6 cut(s) 856, 2934, 2949, 3123, 3138, 4588
HpySE526I ACGT 6 cut(s) 856, 2934, 2949, 3123, 3138, 4588
HspAI GCGC 1 cut(s) 5340
Kpn2I TCCGGA 2 cut(s) 2107, 4039
Ksp22I TGATCA 2 cut(s) 3, 1441
KspAI GTTAAC 1 cut(s) 1413
LguI GCTCTTC 1 cut(s) 465
LmnI GCTCC 6 cut(s) 871, 1618, 3337, 3539, 4343, 5257
Lsp1109I GCAGC 5 cut(s) 1734, 3605, 4516, 4519, 4558
MaeII ACGT 6 cut(s) 856, 2934, 2949, 3123, 3138, 4588
MfeI CAATTG 2 cut(s) 1242, 1887
MhlI GDGCHC 2 cut(s) 927, 2753
MlsI TGGCCA 1 cut(s) 2634
MluNI TGGCCA 1 cut(s) 2634
MmeI TCCRAC 3 cut(s) 3328, 3589, 5262
Mox20I TGGCCA 1 cut(s) 2634
Mph1103I ATGCAT 1 cut(s) 2647
MroI TCCGGA 2 cut(s) 2107, 4039
MroXI GAANNNNTTC 4 cut(s) 152, 723, 2085, 4911
MscI TGGCCA 1 cut(s) 2634
MslI CAYNNNNRTG 7 cut(s) 543, 1209, 2546, 2640, 2994, 3183, 5188
Msp20I TGGCCA 1 cut(s) 2634
MspA1I CMGCKG 3 cut(s) 3035, 3224, 5033
MspI CCGG 4 cut(s) 252, 938, 2108, 4040
MunI CAATTG 2 cut(s) 1242, 1887
Mva1269I GAATGC 2 cut(s) 1582, 4247
MvnI CGCG 1 cut(s) 3420
NciI CCSGG 1 cut(s) 939
NcoI CCATGG 2 cut(s) 2209, 2784
NdeI CATATG 1 cut(s) 3376
NlaIV GGNNCC 9 cut(s) 492, 726, 1620, 2155, 2388, 2427, 2794, 3535, 5027
NmuCI GTSAC 5 cut(s) 852, 1495, 2927, 3116, 3855
NruI TCGCGA 1 cut(s) 3420
NsiI ATGCAT 1 cut(s) 2647
NspI RCATGY 7 cut(s) 2718, 2928, 3117, 3724, 3856, 5128, 5236
NspV TTCGAA 1 cut(s) 533
OliI CACNNNNGTG 2 cut(s) 2994, 3183
PaeR7I CTCGAG 6 cut(s) 1193, 1557, 2876, 3065, 3740, 4411
PagI TCATGA 5 cut(s) 544, 1123, 1444, 2737, 3553
PaqCI CACCTGC 2 cut(s) 4337, 4531
PciI ACATGT 3 cut(s) 2714, 5124, 5232
PciSI GCTCTTC 1 cut(s) 465
PcsI WCGNNNNNNNCGW 2 cut(s) 32, 5226
PctI GAATGC 2 cut(s) 1582, 4247
PdmI GAANNNNTTC 4 cut(s) 152, 723, 2085, 4911
PflFI GACNNNGTC 3 cut(s) 4224, 4235, 5459
PflMI CCANNNNNTGG 1 cut(s) 3380
PfoI TCCNGGA 2 cut(s) 700, 2823
PkrI GCNGC 9 cut(s) 1724, 2802, 2923, 3112, 3533, 3595, 4506, 4509, 4573
PpuMI RGGWCCY 1 cut(s) 2425
PscI ACATGT 3 cut(s) 2714, 5124, 5232
Psp124BI GAGCTC 1 cut(s) 927
Psp5II RGGWCCY 1 cut(s) 2425
PspFI CCCAGC 2 cut(s) 2434, 5029
PspN4I GGNNCC 9 cut(s) 492, 726, 1620, 2155, 2388, 2427, 2794, 3535, 5027
PspPI GGNCC 5 cut(s) 2154, 2425, 4430, 4561, 5262
PspPPI RGGWCCY 1 cut(s) 2425
PspXI VCTCGAGB 2 cut(s) 1193, 4411
PstI CTGCAG 1 cut(s) 1899
PstNI CAGNNNCTG 4 cut(s) 1592, 2173, 4523, 5395
PsyI GACNNNGTC 3 cut(s) 4224, 4235, 5459
PvuII CAGCTG 2 cut(s) 3035, 3224
RruI TCGCGA 1 cut(s) 3420
RseI CAYNNNNRTG 7 cut(s) 543, 1209, 2546, 2640, 2994, 3183, 5188
SacI GAGCTC 1 cut(s) 927
SalI GTCGAC 1 cut(s) 5453
SapI GCTCTTC 1 cut(s) 465
SatI GCNGC 9 cut(s) 1723, 2801, 2922, 3111, 3532, 3594, 4505, 4508, 4572
Sau96I GGNCC 5 cut(s) 2154, 2425, 4430, 4561, 5262
ScaI AGTACT 2 cut(s) 1641, 4243
SduI GDGCHC 2 cut(s) 927, 2753
SfcI CTRYAG 8 cut(s) 1101, 1895, 2610, 2992, 3181, 3292, 3579, 4425
Sfr274I CTCGAG 6 cut(s) 1193, 1557, 2876, 3065, 3740, 4411
SfuI TTCGAA 1 cut(s) 533
SinI GGWCC 3 cut(s) 2154, 2425, 4430
SlaI CTCGAG 6 cut(s) 1193, 1557, 2876, 3065, 3740, 4411
SmiMI CAYNNNNRTG 7 cut(s) 543, 1209, 2546, 2640, 2994, 3183, 5188
SmlI CTYRAG 9 cut(s) 1059, 1193, 1461, 1557, 2876, 3065, 3254, 3740, 4411
SmoI CTYRAG 9 cut(s) 1059, 1193, 1461, 1557, 2876, 3065, 3254, 3740, 4411
SpeI ACTAGT 2 cut(s) 1478, 4498
SspI AATATT 3 cut(s) 562, 1714, 3361
SstI GAGCTC 1 cut(s) 927
StyI CCWWGG 7 cut(s) 712, 2157, 2209, 2784, 3450, 4336, 4454
TaaI ACNGT 9 cut(s) 914, 1052, 1230, 1957, 2059, 4274, 4426, 4437, 5360
TaiI ACGT 6 cut(s) 859, 2937, 2952, 3126, 3141, 4591
TaqII GACCGA 2 cut(s) 1592, 2201
TatI WGTACW 9 cut(s) 580, 1328, 1639, 2164, 2717, 2902, 3091, 3313, 4241
TauI GCSGC 4 cut(s) 2803, 2924, 3113, 3534
TscAI CASTG 9 cut(s) 1143, 1439, 1674, 2076, 2178, 2479, 2635, 3808, 4279
TseFI GTSAC 5 cut(s) 852, 1495, 2927, 3116, 3855
TseI GCWGC 5 cut(s) 1722, 3593, 4504, 4507, 4571
Tsp45I GTSAC 5 cut(s) 852, 1495, 2927, 3116, 3855
TspGWI ACGGA 4 cut(s) 91, 255, 273, 3627
TspRI CASTG 9 cut(s) 1143, 1439, 1674, 2076, 2178, 2479, 2635, 3808, 4279
Tth111I GACNNNGTC 3 cut(s) 4224, 4235, 5459
Van91I CCANNNNNTGG 1 cut(s) 3380
VpaK11BI GGWCC 3 cut(s) 2154, 2425, 4430
XapI RAATTY 7 cut(s) 1417, 2332, 2488, 2762, 5017, 5246, 5432
XbaI TCTAGA 5 cut(s) 1544, 2030, 2743, 4228, 5084
XceI RCATGY 7 cut(s) 2718, 2928, 3117, 3724, 3856, 5128, 5236
XhoI CTCGAG 6 cut(s) 1193, 1557, 2876, 3065, 3740, 4411
XmaJI CCTAGG 1 cut(s) 4454
XmiI GTMKAC 2 cut(s) 1189, 5454
XmnI GAANNNNTTC 4 cut(s) 152, 723, 2085, 4911
ZrmI AGTACT 2 cut(s) 1641, 4243
Zsp2I ATGCAT 1 cut(s) 2647
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.