MD00G1168400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0002394
Physical Location & Seq
Reverse (-)
38663201 .. 38666842
3642 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1168400.v1.1.491

Sequence Viewer

Length: 1539 bp
ATGAGGACCATGAATGATGCTGCCAAGCAATTAAGGGATGATCATAGTTGCTCGAGTGAGGAAGTTGGCCAGGCGTTTTATGCAAGGTCCTATTCTGATTGCCTATTAAATCATAGTTGTCAATCTAGTTGGGATACTAAGAAACCTTATCTGTCTCCGGTAGGGAAACTTTGGGATATAACTGGTTCAAGGACTAGCAGTTCAAGGAAGCGAGGAAGCTTAAATCCGGAGCTTCCCTGCATCAGTGAAGAAAATGAGAATGCAGATGAGGTAGCTGATATTTTCCAAGATGGCATTGTTTCAGATGCATTAAATAGCTCAATACAAAGAGTACCCCTTGCTGACATTCCTGAGATTCCAAACCCTCCTGCATCAGTTTCTAAAGCTGAACCATATGCAGATAGACTTAGTCTAGATTCCGTGAACACTGAAATCAGCTTTACAGGCACTCATAAGAGCTTCAAACAGAGGCTTGGAATCCAAAACAGCAGCAAGAGAAGATATAACAATAAGGAGACAAACGATATTAAGAGGACCACTGGATCACTTCATAACATGTTCAGTAAACCAAAATTATCTGGAAAAACCAGTTTGAGAAAAGGTGGTCCAAGTTTGTCAGAGCAGGAGCCGAAACGTAACAATATCGTGTCCAGCATGACTTCTTTCATTCCACTTGTTCAACAGAAACAAGCAGCTGCAGTTATAACAGGGAATGTTTTGTTTGGAGCTTTAAATTGCTACAAGCGTCTTGCAGAAAAGAAAGAGAATGAACGCAAGATGAAGAAGGAAGCCTTGAAACTTCACCAGTCAAGAATGGAGCAGGAGAATATGAAGCAGTTGGAGCTGCAGAAAAAAAAGAAAGAAGAAGAGAGGAAGAAAAAAGAAGCTGATATGGCATCAAAGAAGAGACAAAGGGAAGAGGAAGAAAGGAAGGAGAAGGAAAGAAAAAGAATGCGTGTTGAAGCCCGTAGACAGCAGAGAGAACATGAATACAAGTTACCTGCTGAAAAAGTAGAGAATGAAATGAAAAGCCAGGCCATTGATGGAAGCGGCCCTGGCGGTAAGAAATCTAAGGATGAAACGCCACATAAGAAAATGGAGGCAGAAAGGGGATTTGACAATTCCAGAAATATTTCGGAGACTGAGCCTAGTACTTCCAGGATTTCAATAAGCAACGCTGGAAGAGAAAGTATTGTCCATGAAGAGTTCCATGAGGCCTGGAGTAATTATGGGTATAAAGCAGAGGTACCGAGCGATTTAGACAAAGAAATGGAGAATTCAATTGGCATTACAAGTCAAGAGCAGTCTTATGACATCTCTCCATACAAAGAATCAGATGATGAAAATGACGATGATGACAATATCATACCGAATAGTAAATTTATTCCTTCATGGTCAAGTAAAAACTGCCTGGCTTTTGCTGTTTCTTGCCAGAATAGAGCAGACCCAGGGACGATCTTCCCCCCAGAAAGCTTTTGCTGCATCTCTGAAGTCCTCTTGCCTCGAAACCATCAATTAAACAACAGCAGAATGCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
INCENP_ARK-bind

Protein Analysis

513

Amino Acids

58.21

Weight (kDa)

8.37

Isoelectric Point (pI)

55.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
INCENP_ARK-bind PF03941 445 - 497 1.8e-06 Inner centromere protein, ARK binding region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 704
Acc36I ACCTGC 1 cut(s) 1009
Acc65I GGTACC 1 cut(s) 1246
AccB1I GGYRCC 1 cut(s) 1246
AccI GTMKAC 1 cut(s) 970
AccIII TCCGGA 1 cut(s) 226
AciI CCGC 2 cut(s) 1050, 1059
AclWI GGATC 1 cut(s) 550
AcoI YGGCCR 1 cut(s) 67
AcsI RAATTY 2 cut(s) 1276, 1379
AcuI CTGAAG 1 cut(s) 1509
AfaI GTAC 3 cut(s) 333, 1153, 1248
AflIII ACRYGT 1 cut(s) 555
AgsI TTSAA 8 cut(s) 189, 204, 463, 680, 796, 962, 1167, 1281
AjnI CCWGG 7 cut(s) 69, 1032, 1054, 1157, 1217, 1410, 1447
AloI GAACNNNNNNTCC 2 cut(s) 184, 216
Alw26I GTCTC 4 cut(s) 159, 509, 901, 1133
AlwI GGATC 1 cut(s) 550
Ama87I CYCGRG 1 cut(s) 52
Aor13HI TCCGGA 1 cut(s) 226
AoxI GGCC 4 cut(s) 67, 1035, 1051, 1215
ApeKI GCWGC 6 cut(s) 20, 489, 692, 695, 844, 1479
ApoI RAATTY 2 cut(s) 1276, 1379
Asp700I GAANNNNTTC 1 cut(s) 1132
Asp718I GGTACC 1 cut(s) 1246
AspS9I GGNCC 5 cut(s) 6, 87, 534, 605, 1052
AsuHPI GGTGA 1 cut(s) 794
AvaI CYCGRG 1 cut(s) 52
AvaII GGWCC 4 cut(s) 6, 87, 534, 605
BalI TGGCCA 1 cut(s) 69
BanI GGYRCC 1 cut(s) 1246
BbvI GCAGC 6 cut(s) 7, 501, 682, 704, 831, 1466
BccI CCATC 3 cut(s) 284, 1037, 1518
BciT130I CCWGG 7 cut(s) 71, 1034, 1056, 1159, 1219, 1412, 1449
BciVI GTATCC 1 cut(s) 127
BclI TGATCA 1 cut(s) 40
BcoDI GTCTC 4 cut(s) 159, 509, 901, 1133
BfaI CTAG 4 cut(s) 126, 195, 413, 1149
BfmI CTRYAG 2 cut(s) 696, 845
BfuAI ACCTGC 1 cut(s) 1009
BfuI GTATCC 1 cut(s) 127
BisI GCNGC 7 cut(s) 21, 490, 693, 696, 845, 1051, 1480
BlsI GCNGC 7 cut(s) 22, 491, 694, 697, 846, 1052, 1481
BmcAI AGTACT 1 cut(s) 1153
Bme1390I CCNGG 7 cut(s) 71, 1034, 1056, 1159, 1219, 1412, 1449
Bme18I GGWCC 4 cut(s) 6, 87, 534, 605
BmeT110I CYCGRG 1 cut(s) 52
BmgT120I GGNCC 5 cut(s) 6, 87, 534, 605, 1052
BmiI GGNNCC 2 cut(s) 627, 1248
BmrFI CCNGG 7 cut(s) 71, 1034, 1056, 1159, 1219, 1412, 1449
BmsI GCATC 6 cut(s) 7, 249, 295, 380, 905, 1491
BpmI CTGGAG 1 cut(s) 1240
BsaJI CCNNGG 3 cut(s) 1054, 1447, 1448
BsaWI WCCGGW 2 cut(s) 157, 226
Bse1I ACTGG 4 cut(s) 187, 544, 588, 805
BseAI TCCGGA 1 cut(s) 226
BseBI CCWGG 7 cut(s) 71, 1034, 1056, 1159, 1219, 1412, 1449
BseDI CCNNGG 3 cut(s) 1054, 1447, 1448
BseGI GGATG 2 cut(s) 43, 1081
BseMII CTCAG 2 cut(s) 342, 1134
BseNI ACTGG 4 cut(s) 187, 544, 588, 805
BseXI GCAGC 6 cut(s) 7, 501, 682, 704, 831, 1466
BshFI GGCC 4 cut(s) 69, 1037, 1053, 1217
BshNI GGYRCC 1 cut(s) 1246
BsiHKCI CYCGRG 1 cut(s) 52
BsiSI CCGG 2 cut(s) 158, 227
BslFI GGGAC 1 cut(s) 1465
BsmAI GTCTC 4 cut(s) 159, 509, 901, 1133
BsmFI GGGAC 1 cut(s) 1465
BsmI GAATGC 3 cut(s) 265, 957, 1536
BsnI GGCC 4 cut(s) 69, 1037, 1053, 1217
BsoBI CYCGRG 1 cut(s) 52
Bsp13I TCCGGA 1 cut(s) 226
Bsp143I GATC 3 cut(s) 40, 542, 1455
BspACI CCGC 2 cut(s) 1050, 1059
BspANI GGCC 4 cut(s) 69, 1037, 1053, 1217
BspCNI CTCAG 2 cut(s) 343, 1135
BspEI TCCGGA 1 cut(s) 226
BspLI GGNNCC 2 cut(s) 627, 1248
BspMAI CTGCAG 2 cut(s) 700, 849
BspMI ACCTGC 1 cut(s) 1009
BspPI GGATC 1 cut(s) 550
BspT107I GGYRCC 1 cut(s) 1246
BsrI ACTGG 4 cut(s) 187, 544, 588, 805
BssECI CCNNGG 3 cut(s) 1054, 1447, 1448
BssMI GATC 3 cut(s) 40, 542, 1455
Bst2UI CCWGG 7 cut(s) 71, 1034, 1056, 1159, 1219, 1412, 1449
Bst6I CTCTTC 5 cut(s) 861, 899, 912, 1177, 1197
BstDEI CTNAG 5 cut(s) 138, 351, 407, 1071, 1143
BstF5I GGATG 2 cut(s) 43, 1081
BstKTI GATC 3 cut(s) 43, 545, 1458
BstMAI GTCTC 4 cut(s) 159, 509, 901, 1133
BstMBI GATC 3 cut(s) 40, 542, 1455
BstMWI GCNNNNNNNGC 6 cut(s) 80, 444, 841, 893, 1056, 1479
BstNI CCWGG 7 cut(s) 71, 1034, 1056, 1159, 1219, 1412, 1449
BstNSI RCATGY 1 cut(s) 559
BstSCI CCNGG 7 cut(s) 69, 1032, 1054, 1157, 1217, 1410, 1447
BstSFI CTRYAG 2 cut(s) 696, 845
BstV1I GCAGC 6 cut(s) 7, 501, 682, 704, 831, 1466
BsuI GTATCC 1 cut(s) 127
BsuRI GGCC 4 cut(s) 69, 1037, 1053, 1217
BtsCI GGATG 2 cut(s) 43, 1081
BtsIMutI CAGTG 3 cut(s) 250, 426, 537
BveI ACCTGC 1 cut(s) 1009
Cfr13I GGNCC 5 cut(s) 6, 87, 534, 605, 1052
CseI GACGC 1 cut(s) 734
Csp6I GTAC 3 cut(s) 332, 1152, 1247
CviAII CATG 7 cut(s) 10, 556, 655, 986, 1199, 1211, 1392
CviQI GTAC 3 cut(s) 332, 1152, 1247
DdeI CTNAG 5 cut(s) 138, 351, 407, 1071, 1143
DpnI GATC 3 cut(s) 42, 544, 1457
DpnII GATC 3 cut(s) 40, 542, 1455
DraI TTTAAA 1 cut(s) 732
EaeI YGGCCR 1 cut(s) 67
Eam1104I CTCTTC 5 cut(s) 861, 899, 912, 1177, 1197
EarI CTCTTC 5 cut(s) 861, 899, 912, 1177, 1197
Eco147I AGGCCT 1 cut(s) 1217
Eco47I GGWCC 4 cut(s) 6, 87, 534, 605
Eco57I CTGAAG 1 cut(s) 1509
Eco88I CYCGRG 1 cut(s) 52
EcoO109I RGGNCCY 1 cut(s) 87
EcoRI GAATTC 1 cut(s) 1276
EcoRII CCWGG 7 cut(s) 69, 1032, 1054, 1157, 1217, 1410, 1447
EcoT22I ATGCAT 1 cut(s) 310
FaeI CATG 7 cut(s) 13, 559, 658, 989, 1202, 1214, 1395
FalI AAGNNNNNCTT 2 cut(s) 776, 808
FaqI GGGAC 1 cut(s) 1465
FatI CATG 7 cut(s) 9, 555, 654, 985, 1198, 1210, 1391
FauNDI CATATG 1 cut(s) 394
FbaI TGATCA 1 cut(s) 40
FblI GTMKAC 1 cut(s) 970
Fnu4HI GCNGC 7 cut(s) 21, 490, 693, 696, 845, 1051, 1480
FokI GGATG 2 cut(s) 50, 1088
Fsp4HI GCNGC 7 cut(s) 21, 490, 693, 696, 845, 1051, 1480
FspBI CTAG 4 cut(s) 126, 195, 413, 1149
GluI GCNGC 7 cut(s) 21, 490, 693, 696, 845, 1051, 1480
GsuI CTGGAG 1 cut(s) 1240
HaeIII GGCC 4 cut(s) 69, 1037, 1053, 1217
HapII CCGG 2 cut(s) 158, 227
HgaI GACGC 1 cut(s) 734
Hin1II CATG 7 cut(s) 13, 559, 658, 989, 1202, 1214, 1395
HindIII AAGCTT 2 cut(s) 217, 1471
HinfI GANTC 4 cut(s) 355, 416, 477, 1331
HpaII CCGG 2 cut(s) 158, 227
HphI GGTGA 1 cut(s) 794
Hpy166II GTNNAC 3 cut(s) 424, 566, 971
Hpy188I TCNGA 6 cut(s) 97, 304, 619, 1138, 1336, 1489
Hpy188III TCNNGA 7 cut(s) 227, 350, 413, 579, 810, 1125, 1298
Hpy8I GTNNAC 3 cut(s) 424, 566, 971
HpyAV CCTTC 4 cut(s) 778, 925, 931, 1398
HpyCH4IV ACGT 1 cut(s) 634
HpyF10VI GCNNNNNNNGC 6 cut(s) 80, 444, 841, 893, 1056, 1479
HpyF3I CTNAG 5 cut(s) 138, 351, 407, 1071, 1143
HpySE526I ACGT 1 cut(s) 634
Hsp92II CATG 7 cut(s) 13, 559, 658, 989, 1202, 1214, 1395
Kpn2I TCCGGA 1 cut(s) 226
KpnI GGTACC 1 cut(s) 1250
Ksp22I TGATCA 1 cut(s) 40
Kzo9I GATC 3 cut(s) 40, 542, 1455
LmnI GCTCC 5 cut(s) 229, 625, 725, 817, 841
Lsp1109I GCAGC 6 cut(s) 7, 501, 682, 704, 831, 1466
LweI GCATC 6 cut(s) 7, 249, 295, 380, 905, 1491
MaeI CTAG 4 cut(s) 126, 195, 413, 1149
MaeII ACGT 1 cut(s) 634
MaeIII GTNAC 2 cut(s) 635, 996
MalI GATC 3 cut(s) 42, 544, 1457
MboI GATC 3 cut(s) 40, 542, 1455
MfeI CAATTG 1 cut(s) 1281
MlsI TGGCCA 1 cut(s) 69
MluCI AATT 9 cut(s) 29, 572, 733, 1120, 1225, 1276, 1281, 1379, 1514
MluNI TGGCCA 1 cut(s) 69
MmeI TCCRAC 1 cut(s) 819
Mox20I TGGCCA 1 cut(s) 69
Mph1103I ATGCAT 1 cut(s) 310
MroI TCCGGA 1 cut(s) 226
MroXI GAANNNNTTC 1 cut(s) 1132
MscI TGGCCA 1 cut(s) 69
MseI TTAA 8 cut(s) 32, 107, 221, 311, 528, 731, 1517, 1537
Msp20I TGGCCA 1 cut(s) 69
MspA1I CMGCKG 1 cut(s) 695
MspI CCGG 2 cut(s) 158, 227
MspR9I CCNGG 7 cut(s) 71, 1034, 1056, 1159, 1219, 1412, 1449
MunI CAATTG 1 cut(s) 1281
Mva1269I GAATGC 3 cut(s) 265, 957, 1536
MvaI CCWGG 7 cut(s) 71, 1034, 1056, 1159, 1219, 1412, 1449
MwoI GCNNNNNNNGC 6 cut(s) 80, 444, 841, 893, 1056, 1479
NdeI CATATG 1 cut(s) 394
NdeII GATC 3 cut(s) 40, 542, 1455
NlaIII CATG 7 cut(s) 13, 559, 658, 989, 1202, 1214, 1395
NlaIV GGNNCC 2 cut(s) 627, 1248
NsiI ATGCAT 1 cut(s) 310
NspI RCATGY 1 cut(s) 559
PaeR7I CTCGAG 1 cut(s) 52
PasI CCCWGGG 1 cut(s) 1448
PceI AGGCCT 1 cut(s) 1217
PciI ACATGT 1 cut(s) 555
PctI GAATGC 3 cut(s) 265, 957, 1536
PdmI GAANNNNTTC 1 cut(s) 1132
PfeI GAWTC 4 cut(s) 355, 416, 477, 1331
PflFI GACNNNGTC 1 cut(s) 408
PfoI TCCNGGA 1 cut(s) 1157
PkrI GCNGC 7 cut(s) 22, 491, 694, 697, 846, 1052, 1481
PpuMI RGGWCCY 1 cut(s) 87
PscI ACATGT 1 cut(s) 555
PsiI TTATAA 1 cut(s) 704
Psp5II RGGWCCY 1 cut(s) 87
Psp6I CCWGG 7 cut(s) 69, 1032, 1054, 1157, 1217, 1410, 1447
PspGI CCWGG 7 cut(s) 69, 1032, 1054, 1157, 1217, 1410, 1447
PspN4I GGNNCC 2 cut(s) 627, 1248
PspPI GGNCC 5 cut(s) 6, 87, 534, 605, 1052
PspPPI RGGWCCY 1 cut(s) 87
PspXI VCTCGAGB 1 cut(s) 52
PstI CTGCAG 2 cut(s) 700, 849
PsyI GACNNNGTC 1 cut(s) 408
PvuII CAGCTG 1 cut(s) 695
RsaI GTAC 3 cut(s) 333, 1153, 1248
RsaNI GTAC 3 cut(s) 332, 1152, 1247
SaqAI TTAA 8 cut(s) 32, 107, 221, 311, 528, 731, 1517, 1537
SatI GCNGC 7 cut(s) 21, 490, 693, 696, 845, 1051, 1480
Sau3AI GATC 3 cut(s) 40, 542, 1455
Sau96I GGNCC 5 cut(s) 6, 87, 534, 605, 1052
ScaI AGTACT 1 cut(s) 1153
ScrFI CCNGG 7 cut(s) 71, 1034, 1056, 1159, 1219, 1412, 1449
SfaNI GCATC 6 cut(s) 7, 249, 295, 380, 905, 1491
SfcI CTRYAG 2 cut(s) 696, 845
Sfr274I CTCGAG 1 cut(s) 52
SinI GGWCC 4 cut(s) 6, 87, 534, 605
SlaI CTCGAG 1 cut(s) 52
SmlI CTYRAG 1 cut(s) 52
SmoI CTYRAG 1 cut(s) 52
Sse9I AATT 9 cut(s) 29, 572, 733, 1120, 1225, 1276, 1281, 1379, 1514
SseBI AGGCCT 1 cut(s) 1217
SsiI CCGC 2 cut(s) 1050, 1059
SspI AATATT 1 cut(s) 1132
SspMI CTAG 4 cut(s) 126, 195, 413, 1149
StuI AGGCCT 1 cut(s) 1217
StyD4I CCNGG 7 cut(s) 69, 1032, 1054, 1157, 1217, 1410, 1447
TaiI ACGT 1 cut(s) 637
TaqI TCGA 2 cut(s) 53, 1504
TasI AATT 9 cut(s) 29, 572, 733, 1120, 1225, 1276, 1281, 1379, 1514
TatI WGTACW 1 cut(s) 1151
TauI GCSGC 1 cut(s) 1053
TfiI GAWTC 4 cut(s) 355, 416, 477, 1331
Tru1I TTAA 8 cut(s) 32, 107, 221, 311, 528, 731, 1517, 1537
Tru9I TTAA 8 cut(s) 32, 107, 221, 311, 528, 731, 1517, 1537
TscAI CASTG 3 cut(s) 250, 433, 544
TseI GCWGC 6 cut(s) 20, 489, 692, 695, 844, 1479
TspGWI ACGGA 1 cut(s) 409
TspRI CASTG 3 cut(s) 250, 433, 544
Tth111I GACNNNGTC 1 cut(s) 408
VpaK11BI GGWCC 4 cut(s) 6, 87, 534, 605
XapI RAATTY 2 cut(s) 1276, 1379
XbaI TCTAGA 1 cut(s) 412
XceI RCATGY 1 cut(s) 559
XcmI CCANNNNNNNNNTGG 1 cut(s) 1040
XhoI CTCGAG 1 cut(s) 52
XmiI GTMKAC 1 cut(s) 970
XmnI GAANNNNTTC 1 cut(s) 1132
XspI CTAG 4 cut(s) 126, 195, 413, 1149
ZrmI AGTACT 1 cut(s) 1153
Zsp2I ATGCAT 1 cut(s) 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.