MD00G1168200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0002394
Physical Location & Seq
Reverse (-)
38649107 .. 38651570
2464 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1168200.v1.1.491

Sequence Viewer

Length: 582 bp
ATGCATGTTGAAGCTCGTAGACAGCGGAGAGAACATGAAGACAAGTTACCTGCTGAAAAACTAGAGAAAGAAATGAAAAACCACTCCACTGATGGAAGAAGCCCTGGCGGTAAGAAATTTAAAATGGACAAAGAAAGGGGATTTGACAATTCCAGAAATAGTTTGGAGACTGAGCCTAGTACTTCAAGGATTTCAATAAGCAATGCCGGAAGAGAAAATATTATCCACGAAAAGTTCCATGAGGCCTCGAGTAGTTATGGATATAAAGCAGAGGTACCTAGCAATTTAGACAGAGAAATGGAGAATTCACTTGGCATTACAAGTCAAGTGCAGTATTATGACATCTCTGCATACAAAGAATCTGATGATGAAAATGACGATGACGATAATGTCATACCAAATAGTAAATTTATTCCTTCATGGTTAAGTAAAAACTGCCTGGCTTTTGCCGTTTCTAGCCAGAATAGAGCAGATCCAAAGACGATATTCGCCCCCCCCCCCCCCCCCCCAGAAAGCTTTTGCTGCATCTCTGAAGTCCTCTTGCCCGAAAAGCATCAATTAAACAACAGCGGAATGTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
INCENP_ARK-bind

Protein Analysis

194

Amino Acids

21.88

Weight (kDa)

5.44

Isoelectric Point (pI)

54.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
INCENP_ARK-bind PF03941 121 - 169 2.3e-08 Inner centromere protein, ARK binding region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 389
Acc36I ACCTGC 1 cut(s) 58
Acc65I GGTACC 1 cut(s) 274
AccB1I GGYRCC 1 cut(s) 274
AccI GTMKAC 1 cut(s) 19
AciI CCGC 3 cut(s) 25, 108, 570
AclWI GGATC 1 cut(s) 467
AcsI RAATTY 3 cut(s) 116, 304, 407
AcuI CTGAAG 1 cut(s) 552
AfaI GTAC 2 cut(s) 181, 276
AgsI TTSAA 3 cut(s) 11, 186, 195
AjnI CCWGG 2 cut(s) 103, 438
AluBI AGCT 2 cut(s) 14, 516
AluI AGCT 2 cut(s) 14, 516
Alw26I GTCTC 1 cut(s) 161
AlwI GGATC 1 cut(s) 467
Ama87I CYCGRG 1 cut(s) 247
AoxI GGCC 1 cut(s) 243
ApeKI GCWGC 1 cut(s) 522
ApoI RAATTY 3 cut(s) 116, 304, 407
Asp718I GGTACC 1 cut(s) 274
AvaI CYCGRG 1 cut(s) 247
BanI GGYRCC 1 cut(s) 274
BarI GAAGNNNNNNTAC 2 cut(s) 30, 62
BbsI GAAGAC 1 cut(s) 45
BbvI GCAGC 1 cut(s) 509
BccI CCATC 1 cut(s) 86
BceAI ACGGC 1 cut(s) 434
BciT130I CCWGG 2 cut(s) 105, 440
BcoDI GTCTC 1 cut(s) 161
BfaI CTAG 4 cut(s) 62, 177, 279, 456
BfuAI ACCTGC 1 cut(s) 58
BisI GCNGC 1 cut(s) 523
BlsI GCNGC 1 cut(s) 524
BmcAI AGTACT 1 cut(s) 181
Bme1390I CCNGG 2 cut(s) 105, 440
BmeT110I CYCGRG 1 cut(s) 247
BmiI GGNNCC 1 cut(s) 276
BmrFI CCNGG 2 cut(s) 105, 440
BmsI GCATC 2 cut(s) 534, 562
BpiI GAAGAC 1 cut(s) 45
BsaJI CCNNGG 1 cut(s) 103
Bse3DI GCAATG 1 cut(s) 208
BseBI CCWGG 2 cut(s) 105, 440
BseDI CCNNGG 1 cut(s) 103
BseMI GCAATG 1 cut(s) 208
BseMII CTCAG 1 cut(s) 162
BseXI GCAGC 1 cut(s) 509
BsgI GTGCAG 1 cut(s) 350
BshFI GGCC 1 cut(s) 245
BshNI GGYRCC 1 cut(s) 274
BsiHKCI CYCGRG 1 cut(s) 247
BsiSI CCGG 1 cut(s) 207
BsmAI GTCTC 1 cut(s) 161
BsnI GGCC 1 cut(s) 245
BsoBI CYCGRG 1 cut(s) 247
Bsp143I GATC 1 cut(s) 472
BspACI CCGC 3 cut(s) 25, 108, 570
BspANI GGCC 1 cut(s) 245
BspCNI CTCAG 1 cut(s) 163
BspLI GGNNCC 1 cut(s) 276
BspMI ACCTGC 1 cut(s) 58
BspPI GGATC 1 cut(s) 467
BspT107I GGYRCC 1 cut(s) 274
BsrDI GCAATG 1 cut(s) 208
BssECI CCNNGG 1 cut(s) 103
BssMI GATC 1 cut(s) 472
Bst2UI CCWGG 2 cut(s) 105, 440
Bst6I CTCTTC 1 cut(s) 205
BstDEI CTNAG 1 cut(s) 171
BstKTI GATC 1 cut(s) 475
BstMAI GTCTC 1 cut(s) 161
BstMBI GATC 1 cut(s) 472
BstMWI GCNNNNNNNGC 3 cut(s) 522, 550, 576
BstNI CCWGG 2 cut(s) 105, 440
BstNSI RCATGY 1 cut(s) 8
BstSCI CCNGG 2 cut(s) 103, 438
BstV1I GCAGC 1 cut(s) 509
BstV2I GAAGAC 1 cut(s) 45
BstX2I RGATCY 1 cut(s) 472
BstYI RGATCY 1 cut(s) 472
BsuRI GGCC 1 cut(s) 245
BtsIMutI CAGTG 1 cut(s) 87
BveI ACCTGC 1 cut(s) 58
Csp6I GTAC 2 cut(s) 180, 275
CviAII CATG 4 cut(s) 5, 35, 239, 420
CviJI RGCY 7 cut(s) 14, 102, 175, 245, 443, 459, 516
CviKI_1 RGCY 7 cut(s) 14, 102, 175, 245, 443, 459, 516
CviQI GTAC 2 cut(s) 180, 275
DdeI CTNAG 1 cut(s) 171
DpnI GATC 1 cut(s) 474
DpnII GATC 1 cut(s) 472
DraI TTTAAA 1 cut(s) 121
DrdI GACNNNNNNGTC 1 cut(s) 389
DseDI GACNNNNNNGTC 1 cut(s) 389
Eam1104I CTCTTC 1 cut(s) 205
EarI CTCTTC 1 cut(s) 205
Eco147I AGGCCT 1 cut(s) 245
Eco57I CTGAAG 1 cut(s) 552
Eco88I CYCGRG 1 cut(s) 247
EcoRI GAATTC 1 cut(s) 304
EcoRII CCWGG 2 cut(s) 103, 438
EcoT22I ATGCAT 1 cut(s) 6
FaeI CATG 4 cut(s) 8, 38, 242, 423
FaiI YATR 9 cut(s) 6, 36, 240, 258, 264, 339, 352, 395, 421
FatI CATG 4 cut(s) 4, 34, 238, 419
FblI GTMKAC 1 cut(s) 19
Fnu4HI GCNGC 1 cut(s) 523
Fsp4HI GCNGC 1 cut(s) 523
FspBI CTAG 4 cut(s) 62, 177, 279, 456
GluI GCNGC 1 cut(s) 523
HaeIII GGCC 1 cut(s) 245
HapII CCGG 1 cut(s) 207
Hin1II CATG 4 cut(s) 8, 38, 242, 423
HindIII AAGCTT 1 cut(s) 514
HinfI GANTC 1 cut(s) 359
HpaII CCGG 1 cut(s) 207
Hpy166II GTNNAC 1 cut(s) 20
Hpy188I TCNGA 2 cut(s) 364, 532
Hpy188III TCNNGA 1 cut(s) 153
Hpy8I GTNNAC 1 cut(s) 20
HpyAV CCTTC 1 cut(s) 426
HpyCH4V TGCA 4 cut(s) 4, 331, 350, 525
HpyF10VI GCNNNNNNNGC 3 cut(s) 522, 550, 576
HpyF3I CTNAG 1 cut(s) 171
Hsp92II CATG 4 cut(s) 8, 38, 242, 423
KpnI GGTACC 1 cut(s) 278
Kzo9I GATC 1 cut(s) 472
LpnPI CCDG 9 cut(s) 63, 90, 117, 166, 220, 425, 452, 473, 522
Lsp1109I GCAGC 1 cut(s) 509
LweI GCATC 2 cut(s) 534, 562
MaeI CTAG 4 cut(s) 62, 177, 279, 456
MaeIII GTNAC 1 cut(s) 45
MalI GATC 1 cut(s) 474
MboI GATC 1 cut(s) 472
MboII GAAGA 3 cut(s) 50, 108, 222
MflI RGATCY 1 cut(s) 472
MluCI AATT 6 cut(s) 116, 148, 283, 304, 407, 557
MnlI CCTC 4 cut(s) 235, 256, 265, 548
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 3 cut(s) 120, 425, 560
MspA1I CMGCKG 2 cut(s) 25, 570
MspI CCGG 1 cut(s) 207
MspR9I CCNGG 2 cut(s) 105, 440
MvaI CCWGG 2 cut(s) 105, 440
MwoI GCNNNNNNNGC 3 cut(s) 522, 550, 576
NdeII GATC 1 cut(s) 472
NlaIII CATG 4 cut(s) 8, 38, 242, 423
NlaIV GGNNCC 1 cut(s) 276
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 8
PaeR7I CTCGAG 1 cut(s) 247
PceI AGGCCT 1 cut(s) 245
PfeI GAWTC 1 cut(s) 359
PkrI GCNGC 1 cut(s) 524
Psp6I CCWGG 2 cut(s) 103, 438
PspGI CCWGG 2 cut(s) 103, 438
PspN4I GGNNCC 1 cut(s) 276
PspXI VCTCGAGB 1 cut(s) 247
PsuI RGATCY 1 cut(s) 472
RsaI GTAC 2 cut(s) 181, 276
RsaNI GTAC 2 cut(s) 180, 275
SaqAI TTAA 3 cut(s) 120, 425, 560
SatI GCNGC 1 cut(s) 523
Sau3AI GATC 1 cut(s) 472
ScaI AGTACT 1 cut(s) 181
ScrFI CCNGG 2 cut(s) 105, 440
SetI ASST 5 cut(s) 16, 52, 276, 280, 518
SfaNI GCATC 2 cut(s) 534, 562
Sfr274I CTCGAG 1 cut(s) 247
SlaI CTCGAG 1 cut(s) 247
SmlI CTYRAG 1 cut(s) 247
SmoI CTYRAG 1 cut(s) 247
Sse9I AATT 6 cut(s) 116, 148, 283, 304, 407, 557
SseBI AGGCCT 1 cut(s) 245
SsiI CCGC 3 cut(s) 25, 108, 570
SspI AATATT 1 cut(s) 220
SspMI CTAG 4 cut(s) 62, 177, 279, 456
StuI AGGCCT 1 cut(s) 245
StyD4I CCNGG 2 cut(s) 103, 438
TaqI TCGA 1 cut(s) 248
TasI AATT 6 cut(s) 116, 148, 283, 304, 407, 557
TatI WGTACW 1 cut(s) 179
TfiI GAWTC 1 cut(s) 359
Tru1I TTAA 3 cut(s) 120, 425, 560
Tru9I TTAA 3 cut(s) 120, 425, 560
TscAI CASTG 1 cut(s) 94
TseI GCWGC 1 cut(s) 522
TspDTI ATGAA 4 cut(s) 51, 89, 384, 408
TspRI CASTG 1 cut(s) 94
XapI RAATTY 3 cut(s) 116, 304, 407
XceI RCATGY 1 cut(s) 8
XcmI CCANNNNNNNNNTGG 2 cut(s) 89, 160
XhoI CTCGAG 1 cut(s) 247
XmiI GTMKAC 1 cut(s) 19
XspI CTAG 4 cut(s) 62, 177, 279, 456
ZrmI AGTACT 1 cut(s) 181
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.