RLG00000029543

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0002394
Physical Location & Seq
Forward (+)
40159699 .. 40167369
7671 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000029543

Sequence Viewer

Length: 5448 bp
ATGTCGACGACTGAGAAGCACTGGTTGCAGATCTTCGAGGGAAAGCAATGGATAATCAACAAAGCCAAGCAGCAGGCCTTCCTCTTCGACCAAGACCTCGCCTCCAAACTCATCATTGCCGGAATTGCCCCTCCTCCTTCTCTTTTCTCCTCCCACCCCGACGTGTTGAATCATCATGAGCTGATTTCAGAAGTTCCACTCCCTTGCACACACCGTGTGATCCCTTTCACTGGTAGTCATTGCTGTGGGTTCGACAACCCAGTTGCTACAGCTCACTACGGGCAGCTAACAAATGATTTGTGCACGGAACATTGTGGGGTAAAGAAAGGTTTTGATATAGGAGGCAATGTATTGGATTTGCCACAGTGCCCAATCAGTAATGGAGGATGCGCCTCCAATGTTCCTCAAGATCATCAAGAAGAGGATCCTAGTGTTACGTCTCCTGAAGATGAGAGGGATGCAAGAGTTTCAGACGCTTACCATGATTCAGCTTTACAACTGGCTAAAGGTGCAGCAAAGGAGGTATCAGCTTTGCCACAGTGCCACATTAGTAATGGTGGGTGTGCTTCTATTGGTGTTCTAAAAGATCAGAAAGAAGAGACATCTGGTGGTACATCTCCTGAAGATCAAAGGAATGCAAAAATGGAACGTATCCCAAAGATCAGTAATGGTGGATGTGCCTCCAATGGCGTTACTCAAGATCATAGAGAAGAGGATCCTAGTGTTACGTCTCCTGAAGATGAGAGGGATGAAAGAGTCTCAGACGTTTACCATGACTCAGCTTTAACACCAGCTAGAGGTACAGGAGAAGGGGTATCAATTTTGCCACAGGGCCACATTAGTGATGCTGGGTGTGCCTTTGATGGTGTTCTACAAGATCACAAAGAAGAAAATCCTGGTGGTACGTCTCCTGAAGATCAGAGGGATGCAAGAATGTTAGACATTTACCTCGACCCAGCTCTTTCACTGGCTAGAGTTCAAAGATCTAAGTCAAGACAAAAGGCTCTGGCAATTCGAAACAGTACACAAAAAAGCTTATCACAGGATAAGGGCAATGCTGGTGAAACTATCAAGTGTTTGATATCCACCCCACAGTCTGAAAATGTCGATGAATTGAATGACAATCATTCTCAAGTTGAAAAACCAAAGATAGGAGAATTTCAAAGCAAGGAAATAGGTTCTGACATCTACTCTGGTAGAATTACTAGATCTAGAAGCTCTGGCCAACAGGAGAACTCTTTAAATGTCAGTGGCTCTCCATGTATTCACAAGACAAGTGATGGTATAGCTGTTAATTCTATTTGCAAATTGACACATTTGTCTGACCATGTAATTCACCCATTGGAATTGGTTAGTTACTCTCATGTAACTAATGACAGTTGTCGAGTGGAGGAATTGAAGCCGGGAGACTACTCAAGCAAGGAGAAACGAACTGTTGAGGAAGATTCTATGTGTGCAAACTTGGAAAGAGAAGTATACACTCCAAAGGCTTTACAAGAATTCAAAGGTCTTTCAATTTCTAAGGATGTTGCTGGGAACTCAATTGTCTGTAGGAGTCCTGTTGAAGAAGCACATCTAAATGAGGATCATCACAAGACGTCTGAATCTTCACCTGAGGCACAGAAGAAAGAGGGTGGAATGGGTTTGGAAGTGGCGGACAATAGTCCAAATGCTCCGTTCACTTTTCTGCATGAAGAAAAAGAGGCATCAATTTTCCCAATTTTGATCAAGCAGGCTGCTGGACATCCTCAAGAGTCTTTGCTGGAGGAAACAGGAGTAGCACTTCCAACAAGCACTAACATTGATGAAGGAAGCCAATGCTTAAAAGAGAACCCAGTTGCTCTTCTTCAGGACAATTTAAGACTAGAAAACGCTGCAGATTTATCTTGGGCTGGAAAAACCATGCAGGCACAGAGATTTGATTTTGGAGGGCCCAGCGAATTTCAAGAATTGTCATGTGGTGCTCCACATGTTAAATCCTCACAGTCTGAAAATGTTGGTGAATTGAATGATAATCATTCTGTAGCGGAAAAACAAAAGATAGGAGAATTTCAAATCAAGGAAGAAGACACTGACATCTACTCTGGTAGAATTACTAGATCTAGAAGCTCTGGCCATCAGGAGAACAGTGGCTCGCCCTGCATTGGCAGGACAACTAGTGGTATAGCAGTTGATATTGATACTGTGGAACAGTCCTGTTTAAAAGGACTTTCTACTCCTGCTAGTAAGAAGGGGATGCAGGGCAGTTCATTAGTAAAATTGCCTCTTTCCTTCATGCATGGGGAAAAGAAACTAGAAGAAGGAATGACCATCACTACCCATGAAAACTGTAACTCATCCCTGGAAATGGATTTTCTGGGCAATTGTGAAGTTTCAGCCAAAGAAATGGAGGTCCAGAGCGGTTTGGTTGAAGCCCTTGAAGAGAAGTTCAGTAAATCTAGAAGAGTGTCACATGGCAATGTGATCTCATCTGTAAAAGAATCATTTGATGCTTATACAGATGCAGTTCCTAATACTTTGCTAGAAAGTGGGAAAACTCCGAGACAGAAATGTTCTATGATAGATGATCAAACTCCCTTGCGAGTGGACTGGGATGGTTTGGTTCATAGCCCGGTAAAGGAAGTAAAGGGGTCTAATTTGTCTACTGCTAATGCTGCAATACCAATGGAGTTGGTTTCAGAAGACTGTGCAGTGGATTCTAATGCTCTTTGTAGTCCTAATAAATCAACAGATGTTGATTTTGCTACGGTGTCTGGGCCTGGTTCTCATAGAATCCTAGATGCAGAGATTCTTGAAGTTCAGAACCCTTGTGCTGCTTTTAGGGATGAGCTGAGGAGTAACTTGGCTCAGTTGACTGTAAATGCACACATTTCTCCAGAATATGTACAAAGGAGTGCCAGTGATGACAGAGAGTTAGGTTGTTCAGAATCAACTGAATGCCAGATAGCAGAGAACTCCAAAGGAAGAAGTTTTTGGTATTCAATGGAGGGTACATCACCTAAGCTCAAACGAAGAAAGATTGACGATAGAAGAGTTCATTACCTGTCTGCTTCAGTAGCCTTGAGAGAAGAGGTTCTTCACGCTGTAAAAAAAGATTCTATGTGTGCAAACTTGGAAAGAGAAGAATACAGTCCAAAGGCTTTACAACAATTCCAAGGGCATTCAATTTCTCAGGAGTATGCTGAGAAATTAATTGTCAGTAAGAGTCCTGCTGAAGAAACACATCTAAATGAGAATCACATGGTAGAGAGGCCTGAATCTTTACCCGAGGCACAGAAGAAAGAGGGTGGAATGGGTTTGGAAGGGGTGGATAATAGTCCAAATGCTCCATTCACTTTTTTGCATGAAGAAAAAGAAGCATCAGTTTTCTCAAGATTGATCGAGCAGGCTGCTGGACATCCTCTAGATTCTTTGCTGGATGGTACAGGAGTATCACTTCCAACTAGCATTAACATTGATAGAGGAAGCCAATGCTTAAAAGAGGACCCACTTTGTCTTCATCTTCAGGATGATTTAAGAGTGGAAAATGTTGAAGATTGGCCTTGTGCTGGAAGAACCATGCTTGCAAAAAGATTTGATTTTGGAGGGACCAGCAACTTTAAAGAATTGTCAGGTGGTGCTCCATATGTTAAATCCTTGGATTTGACTAGTGCGGATGACGCAATGCCTGTACTTGAGAGGTTTGTTATAAAAACAGATGATGAACCATGTAGCATAGCTGACAAGGGAATTAGCTTTGACGAGTGGAACCTTCCAAACACTGCAGTAGAACGTGCCAGTATTCTGGAGCAGCTTTGCAAATCTGCTTGCATGCAAACTCCCGTAGCCTGTTCTTCAGCTTCCTATAAGTTGCATAAAATTCAAAATCTACAGCAATCTGTTCCGACTGGGGTTTTGAAGCATGTAGATCTGAGGACCACATTTCCTATAAATGATAATGTTAAGCAATTAAAGGATGGTAATAGTTGTTGGAGTGAGGGAGTTGGCCCAGCCTTTTATGGAGGATCCTATTCTGATTGCCTACCGAATTTTAGTGGTCAACCTAGTTGGGATATTAAGAAACCTTATTCCTCTCCTGTTGGGAAACTTTGGGATAGAATTGCTTCAAGCTCTAGTAGTTCAGGGAAGCGAGTGAGCTCAATTCCAGAGCTTGCCTGTATTAGTGAAGAAAATGAGAATACAGATGAGGTAGCTGATACTTTTCAAGATGGCATTGTATCAGAAGTGTTAGATTGTTCTCCAAAAAGGCCACTGCTTGCAGACATTACAGAAATCCCTAACCTTCCTGCTTCAGTTTCTAAAGCTGCACCCTACACTGATAGATTTAGTCTAGATTCAGTAACCACCGAATTCAGTTTAACGGGGACTCATAAGAGCGTCAAAAAGAAGCCTGGAATCCAAAACAGCAGTAAGAGAAGATATAACAATAAGGAGAACCAGAGTGTATCACGAAAGAGGGCCACCGGATCACTTCAGAGTAGGTTCAGTAAGCCAAAGTTTTCTGGAAAAACCAGTTTGAGAAAAGGAGGCCCTAGTTTGTCAGAGCTGGAGCCCAAGCGTAACAATATTGTTTCCAATATTACTTCTTTTATTCCACTTGTGCAACAGAAACAAGCAGCTGCAGCCTTACCAGGGAAAAGGGACATCAAAGTGAAGGCCCTGGAGGCTGCTGAAGCTGCAAAGCGTCTTGCAGAAAGGAAAGACAATGAACGTAAGATGAAGAAAGAAGCTATGAAGGTTGAGCGAGCAAGAGTGGAGCAAGAGAATCTGAGGAAGTTGGAGCTGCAGAGGAAAAGGAAAGAAGAGGAGAGGAAGAAAAATGAGGCTGATATGGCAGCAAAGAAGAGAGAAAGGGAAGAGGAAGATAAAAAGGAGAAGCAAAGAAAAAGAATGCGTGTTGAAGAAGGGCGGAGGGAGCAGAGAGAACATGAAGACAAGTTACGTGCGGAAAAAGTAAAGAAAGAAATAAAATGCCAAGCCAGTGATGGAAGAAGATGCGGGAGTAAGGACTCAAAATGCAATACCGTGCATAAGAAAATGGACAAAGAAAGGGAACATGACACTCTTAGGAGCATTTCAGAGACTGATCCTAGGAGTTCCCGTGTTTCAGAAAATAATGCCAGAAGCGGAACAGCTGTCCATGAGGAGTACTATAAGGGCTTCAGTAATTTTGAGCATAAGGAAGAGAGTGATTTAGACAAAGCCACAGAAAATGAGAAGTTGGTTGTGAAGACGAGTCGAGAACAGTCTTATGATATCTCTCCATACAAAGAATCAGATGATGAAAACGATGAAGATGATGATAGTGTACCAAATAATAAATTTATTCCTTTGTGGGCAAGCAAAAATTGCCTGGCTCAGGTTGCTTCTCGCCAGAGTAGAACTGACCCAGAGATGTTCTTCCCTCCCAAAAGCTTTCCCTGTGTATCTGAAGTTCTGTTGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
INCENP_ARK-bind

Protein Analysis

1816

Amino Acids

200.2

Weight (kDa)

5.49

Isoelectric Point (pI)

54.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
INCENP_ARK-bind PF03941 1759 - 1808 1e-06 Inner centromere protein, ARK binding region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3689
AasI GACNNNNNNGTC 1 cut(s) 1318
AatII GACGTC 1 cut(s) 1601
AccBSI CCGCTC 1 cut(s) 2400
AccI GTMKAC 3 cut(s) 5, 1476, 2642
AciI CCGC 8 cut(s) 1655, 2027, 2400, 3653, 4909, 4946, 4998, 5127
AcoI YGGCCR 2 cut(s) 1222, 2113
AcsI RAATTY 8 cut(s) 1157, 1499, 1940, 2048, 3858, 4027, 4349, 5321
AcyI GRCGYC 1 cut(s) 1598
AdeI CACNNNGTG 2 cut(s) 215, 217
AfiI CCNNNNNNNGG 9 cut(s) 230, 797, 1151, 2144, 2347, 2617, 3548, 4632, 5359
AflIII ACRYGT 2 cut(s) 162, 1969
AhlI ACTAGT 2 cut(s) 2156, 3647
AjiI CACGTC 1 cut(s) 163
AjnI CCWGG 7 cut(s) 895, 2340, 2758, 4390, 4630, 4659, 5352
AjuI GAANNNNNNNTTGG 2 cut(s) 2956, 2988
AleI CACNNNNGTG 1 cut(s) 840
Alw21I GWGCWC 4 cut(s) 305, 1966, 3622, 4139
Alw26I GTCTC 8 cut(s) 444, 593, 735, 763, 912, 1401, 2536, 5075
Alw44I GTGCAC 1 cut(s) 301
AlwNI CAGNNNCTG 1 cut(s) 5084
Ama87I CYCGRG 1 cut(s) 3266
ApaI GGGCCC 1 cut(s) 1935
ApaLI GTGCAC 1 cut(s) 301
ApoI RAATTY 8 cut(s) 1157, 1499, 1940, 2048, 3858, 4027, 4349, 5321
ArsI GACNNNNNNTTYG 2 cut(s) 3877, 3909
AseI ATTAAT 1 cut(s) 3191
Asp700I GAANNNNTTC 2 cut(s) 3072, 4102
AspA2I CCTAGG 1 cut(s) 5090
AspLEI GCGC 1 cut(s) 392
AsuC2I CCSGG 2 cut(s) 1404, 2612
AsuHPI GGTGA 5 cut(s) 1073, 1328, 1602, 2012, 2988
AsuII TTCGAA 1 cut(s) 1015
AvaI CYCGRG 1 cut(s) 3266
AvaII GGWCC 4 cut(s) 2392, 3484, 3588, 3915
AvrII CCTAGG 1 cut(s) 5090
AxyI CCTNAGG 1 cut(s) 1614
BaeGI GKGCMC 3 cut(s) 305, 371, 1935
BaeI ACNNNNGTAYC 4 cut(s) 3414, 3414, 3447, 3447
BalI TGGCCA 2 cut(s) 1224, 2115
BamHI GGATCC 3 cut(s) 424, 715, 4004
BanII GRGCYC 3 cut(s) 1935, 4139, 4554
BarI GAAGNNNNNNTAC 2 cut(s) 4923, 4955
BbsI GAAGAC 5 cut(s) 2073, 2688, 3488, 4938, 5237
Bbv12I GWGCWC 4 cut(s) 305, 1966, 3622, 4139
BbvCI CCTCAGC 1 cut(s) 2831
BccI CCATC 9 cut(s) 857, 1274, 2124, 2318, 2588, 3413, 3950, 4202, 4979
BcgI CGANNNNNNTGC 2 cut(s) 3371, 3405
BciT130I CCWGG 7 cut(s) 897, 2342, 2760, 4392, 4632, 4661, 5354
BciVI GTATCC 1 cut(s) 662
BclI TGATCA 2 cut(s) 1725, 2566
BcnI CCSGG 2 cut(s) 1404, 2612
BcoDI GTCTC 8 cut(s) 444, 593, 735, 763, 912, 1401, 2536, 5075
BcuI ACTAGT 2 cut(s) 2156, 3647
BfmI CTRYAG 8 cut(s) 267, 1549, 1875, 2022, 3762, 3869, 4620, 4784
BfuI GTATCC 1 cut(s) 662
BglI GCCNNNNNGGC 1 cut(s) 4664
BglII AGATCT 5 cut(s) 30, 983, 1208, 2099, 3907
BlnI CCTAGG 1 cut(s) 5090
BmcAI AGTACT 1 cut(s) 5150
Bme1390I CCNGG 9 cut(s) 897, 1404, 2342, 2612, 2760, 4392, 4632, 4661, 5354
Bme18I GGWCC 4 cut(s) 2392, 3484, 3588, 3915
BmeT110I CYCGRG 1 cut(s) 3266
BmgBI CACGTC 1 cut(s) 163
BmiI GGNNCC 8 cut(s) 426, 717, 1933, 3486, 3589, 3749, 4006, 4551
BmrFI CCNGG 9 cut(s) 897, 1404, 2342, 2612, 2760, 4392, 4632, 4661, 5354
BmrI ACTGGG 4 cut(s) 254, 1829, 2599, 3897
BmuI ACTGGG 4 cut(s) 254, 1829, 2599, 3897
BoxI GACNNNNGTC 2 cut(s) 1380, 1662
BpiI GAAGAC 5 cut(s) 2073, 2688, 3488, 4938, 5237
BpmI CTGGAG 5 cut(s) 1784, 2859, 3806, 4568, 4682
Bpu10I CCTNAGC 3 cut(s) 2831, 3000, 5358
Bpu14I TTCGAA 1 cut(s) 1015
BpuEI CTTGAG 8 cut(s) 390, 681, 1116, 1399, 1734, 3082, 3355, 3695
BpuMI CCSGG 2 cut(s) 1404, 2612
BsaAI YACGTR 1 cut(s) 4943
BsaHI GRCGYC 1 cut(s) 1598
BsaJI CCNNGG 7 cut(s) 2340, 3154, 3267, 3636, 4631, 4659, 5090
BsaWI WCCGGW 1 cut(s) 4463
Bsc4I CCNNNNNNNGG 9 cut(s) 230, 797, 1151, 2144, 2347, 2617, 3548, 4632, 5359
Bse21I CCTNAGG 1 cut(s) 1614
Bse3DI GCAATG 7 cut(s) 53, 114, 238, 352, 1060, 2464, 3669
BseBI CCWGG 7 cut(s) 897, 2342, 2760, 4392, 4632, 4661, 5354
BseDI CCNNGG 7 cut(s) 2340, 3154, 3267, 3636, 4631, 4659, 5090
BseLI CCNNNNNNNGG 9 cut(s) 230, 797, 1151, 2144, 2347, 2617, 3548, 4632, 5359
BseMI GCAATG 7 cut(s) 53, 114, 238, 352, 1060, 2464, 3669
BseRI GAGGAG 5 cut(s) 123, 139, 2848, 4820, 5159
BseSI GKGCMC 3 cut(s) 305, 371, 1935
BseYI CCCAGC 5 cut(s) 848, 955, 1532, 1934, 3988
BsgI GTGCAG 3 cut(s) 531, 2709, 4290
BsiHKAI GWGCWC 4 cut(s) 305, 1966, 3622, 4139
BsiHKCI CYCGRG 1 cut(s) 3266
BsiSI CCGG 4 cut(s) 120, 1403, 2612, 4464
BslFI GGGAC 3 cut(s) 3601, 4378, 4655
BslI CCNNNNNNNGG 9 cut(s) 230, 797, 1151, 2144, 2347, 2617, 3548, 4632, 5359
BsmAI GTCTC 8 cut(s) 444, 593, 735, 763, 912, 1401, 2536, 5075
BsmBI CGTCTC 3 cut(s) 444, 735, 912
BsmFI GGGAC 3 cut(s) 3601, 4378, 4655
BsmI GAATGC 4 cut(s) 640, 2942, 3160, 4896
BsoBI CYCGRG 1 cut(s) 3266
Bsp119I TTCGAA 1 cut(s) 1015
Bsp120I GGGCCC 1 cut(s) 1931
Bsp1286I GDGCHC 7 cut(s) 305, 371, 1935, 1966, 3622, 4139, 4554
Bsp1407I TGTACA 1 cut(s) 2884
BspACI CCGC 8 cut(s) 1655, 2027, 2400, 3653, 4909, 4946, 4998, 5127
BspHI TCATGA 1 cut(s) 175
BspLI GGNNCC 8 cut(s) 426, 717, 1933, 3486, 3589, 3749, 4006, 4551
BspMAI CTGCAG 4 cut(s) 1879, 3766, 4624, 4788
BspQI GCTCTTC 1 cut(s) 1848
BspT104I TTCGAA 1 cut(s) 1015
BsrBI CCGCTC 1 cut(s) 2400
BsrDI GCAATG 7 cut(s) 53, 114, 238, 352, 1060, 2464, 3669
BsrGI TGTACA 1 cut(s) 2884
BssECI CCNNGG 7 cut(s) 2340, 3154, 3267, 3636, 4631, 4659, 5090
BssNAI GTATAC 1 cut(s) 1477
BssNI GRCGYC 1 cut(s) 1598
BssT1I CCWWGG 3 cut(s) 3154, 3636, 5090
Bst1107I GTATAC 1 cut(s) 1477
Bst2UI CCWGG 7 cut(s) 897, 2342, 2760, 4392, 4632, 4661, 5354
BstACI GRCGYC 1 cut(s) 1598
BstAPI GCANNNNNTGC 2 cut(s) 25, 5349
BstAUI TGTACA 1 cut(s) 2884
BstBAI YACGTR 1 cut(s) 4943
BstBI TTCGAA 1 cut(s) 1015
BstENI CCTNNNNNAGG 1 cut(s) 5357
BstHHI GCGC 1 cut(s) 392
BstMAI GTCTC 8 cut(s) 444, 593, 735, 763, 912, 1401, 2536, 5075
BstNI CCWGG 7 cut(s) 897, 2342, 2760, 4392, 4632, 4661, 5354
BstNSI RCATGY 3 cut(s) 1973, 3814, 3905
BstPAI GACNNNNGTC 2 cut(s) 1380, 1662
BstSCI CCNGG 9 cut(s) 895, 1402, 2340, 2610, 2758, 4390, 4630, 4659, 5352
BstSFI CTRYAG 8 cut(s) 267, 1549, 1875, 2022, 3762, 3869, 4620, 4784
BstSLI GKGCMC 3 cut(s) 305, 371, 1935
BstV2I GAAGAC 5 cut(s) 2073, 2688, 3488, 4938, 5237
BstX2I RGATCY 8 cut(s) 30, 424, 715, 983, 1208, 2099, 3907, 4004
BstXI CCANNNNNNTGG 2 cut(s) 2386, 3784
BstYI RGATCY 8 cut(s) 30, 424, 715, 983, 1208, 2099, 3907, 4004
BstZ17I GTATAC 1 cut(s) 1477
Bsu36I CCTNAGG 1 cut(s) 1614
BsuI GTATCC 1 cut(s) 662
BtrI CACGTC 1 cut(s) 163
BtsI GCAGTG 3 cut(s) 2697, 3759, 4250
CaiI CAGNNNCTG 1 cut(s) 5084
CciI TCATGA 1 cut(s) 175
CfoI GCGC 1 cut(s) 392
CseI GACGC 4 cut(s) 482, 3668, 4366, 4673
DraI TTTAAA 3 cut(s) 1242, 2202, 3601
DraIII CACNNNGTG 2 cut(s) 215, 217
DrdI GACNNNNNNGTC 1 cut(s) 1318
DseDI GACNNNNNNGTC 1 cut(s) 1318
EaeI YGGCCR 2 cut(s) 1222, 2113
EciI GGCGGA 2 cut(s) 1670, 4924
Ecl136II GAGCTC 1 cut(s) 4137
Eco130I CCWWGG 3 cut(s) 3154, 3636, 5090
Eco147I AGGCCT 2 cut(s) 77, 3253
Eco24I GRGCYC 3 cut(s) 1935, 4139, 4554
Eco32I GATATC 2 cut(s) 1083, 5257
Eco47I GGWCC 4 cut(s) 2392, 3484, 3588, 3915
Eco53kI GAGCTC 1 cut(s) 4137
Eco81I CCTNAGG 1 cut(s) 1614
Eco88I CYCGRG 1 cut(s) 3266
EcoICRI GAGCTC 1 cut(s) 4137
EcoNI CCTNNNNNAGG 1 cut(s) 5357
EcoO109I RGGNCCY 4 cut(s) 1931, 3484, 4529, 4657
EcoRI GAATTC 2 cut(s) 1499, 4349
EcoRII CCWGG 7 cut(s) 895, 2340, 2758, 4390, 4630, 4659, 5352
EcoRV GATATC 2 cut(s) 1083, 5257
EcoT14I CCWWGG 3 cut(s) 3154, 3636, 5090
EcoT22I ATGCAT 1 cut(s) 2280
EcoT38I GRGCYC 3 cut(s) 1935, 4139, 4554
ErhI CCWWGG 3 cut(s) 3154, 3636, 5090
Esp3I CGTCTC 3 cut(s) 444, 735, 912
FalI AAGNNNNNCTT 2 cut(s) 3060, 3092
FaqI GGGAC 3 cut(s) 3601, 4378, 4655
FauI CCCGC 1 cut(s) 4991
FauNDI CATATG 1 cut(s) 3625
FbaI TGATCA 2 cut(s) 1725, 2566
FblI GTMKAC 3 cut(s) 5, 1476, 2642
FriOI GRGCYC 3 cut(s) 1935, 4139, 4554
GlaI GCGC 1 cut(s) 391
GsaI CCCAGC 5 cut(s) 852, 959, 1536, 1938, 3992
GsuI CTGGAG 5 cut(s) 1784, 2859, 3806, 4568, 4682
HapII CCGG 4 cut(s) 120, 1403, 2612, 4464
HgaI GACGC 4 cut(s) 482, 3668, 4366, 4673
HhaI GCGC 1 cut(s) 392
Hin1I GRCGYC 1 cut(s) 1598
Hin6I GCGC 1 cut(s) 390
HinP1I GCGC 1 cut(s) 390
HincII GTYRAC 3 cut(s) 6, 2853, 4040
HindII GTYRAC 3 cut(s) 6, 2853, 4040
HindIII AAGCTT 2 cut(s) 1033, 5413
HpaII CCGG 4 cut(s) 120, 1403, 2612, 4464
HphI GGTGA 5 cut(s) 1073, 1328, 1602, 2012, 2988
Hpy99I CGWCG 2 cut(s) 10, 164
Hsp92I GRCGYC 1 cut(s) 1598
HspAI GCGC 1 cut(s) 390
Ksp22I TGATCA 2 cut(s) 1725, 2566
LguI GCTCTTC 1 cut(s) 1848
MbiI CCGCTC 1 cut(s) 2400
MfeI CAATTG 2 cut(s) 1542, 2362
MflI RGATCY 8 cut(s) 30, 424, 715, 983, 1208, 2099, 3907, 4004
MhlI GDGCHC 7 cut(s) 305, 371, 1935, 1966, 3622, 4139, 4554
MlsI TGGCCA 2 cut(s) 1224, 2115
MluNI TGGCCA 2 cut(s) 1224, 2115
MlyI GAGTC 8 cut(s) 765, 770, 1564, 1763, 3214, 4360, 5003, 5245
MmeI TCCRAC 5 cut(s) 1811, 3464, 3908, 3950, 4758
Mox20I TGGCCA 2 cut(s) 1224, 2115
Mph1103I ATGCAT 1 cut(s) 2280
MroXI GAANNNNTTC 2 cut(s) 3072, 4102
MscI TGGCCA 2 cut(s) 1224, 2115
MslI CAYNNNNRTG 6 cut(s) 243, 840, 1578, 2457, 3228, 4649
Msp20I TGGCCA 2 cut(s) 1224, 2115
MspA1I CMGCKG 2 cut(s) 4619, 5135
MspI CCGG 4 cut(s) 120, 1403, 2612, 4464
MspR9I CCNGG 9 cut(s) 897, 1404, 2342, 2612, 2760, 4392, 4632, 4661, 5354
MunI CAATTG 2 cut(s) 1542, 2362
Mva1269I GAATGC 4 cut(s) 640, 2942, 3160, 4896
MvaI CCWGG 7 cut(s) 897, 2342, 2760, 4392, 4632, 4661, 5354
NciI CCSGG 2 cut(s) 1404, 2612
NdeI CATATG 1 cut(s) 3625
NlaIV GGNNCC 8 cut(s) 426, 717, 1933, 3486, 3589, 3749, 4006, 4551
NmuCI GTSAC 1 cut(s) 2448
NsiI ATGCAT 1 cut(s) 2280
NspI RCATGY 3 cut(s) 1973, 3814, 3905
NspV TTCGAA 1 cut(s) 1015
OliI CACNNNNGTG 1 cut(s) 840
PaeI GCATGC 1 cut(s) 3814
PagI TCATGA 1 cut(s) 175
PceI AGGCCT 2 cut(s) 77, 3253
PciI ACATGT 1 cut(s) 1969
PciSI GCTCTTC 1 cut(s) 1848
PctI GAATGC 4 cut(s) 640, 2942, 3160, 4896
PdmI GAANNNNTTC 2 cut(s) 3072, 4102
PleI GAGTC 8 cut(s) 764, 770, 1563, 1762, 3213, 4360, 5003, 5244
PpsI GAGTC 8 cut(s) 764, 770, 1563, 1762, 3213, 4360, 5003, 5244
Ppu21I YACGTR 1 cut(s) 4943
PpuMI RGGWCCY 1 cut(s) 3484
PscI ACATGT 1 cut(s) 1969
PshAI GACNNNNGTC 2 cut(s) 1380, 1662
PshBI ATTAAT 1 cut(s) 3191
PsiI TTATAA 1 cut(s) 3689
Psp124BI GAGCTC 1 cut(s) 4139
Psp5II RGGWCCY 1 cut(s) 3484
Psp6I CCWGG 7 cut(s) 895, 2340, 2758, 4390, 4630, 4659, 5352
PspFI CCCAGC 5 cut(s) 848, 955, 1532, 1934, 3988
PspGI CCWGG 7 cut(s) 895, 2340, 2758, 4390, 4630, 4659, 5352
PspN4I GGNNCC 8 cut(s) 426, 717, 1933, 3486, 3589, 3749, 4006, 4551
PspOMI GGGCCC 1 cut(s) 1931
PspPPI RGGWCCY 1 cut(s) 3484
PsrI GAACNNNNNNTAC 1 cut(s) 5418
PstI CTGCAG 4 cut(s) 1879, 3766, 4624, 4788
PstNI CAGNNNCTG 1 cut(s) 5084
PsuI RGATCY 8 cut(s) 30, 424, 715, 983, 1208, 2099, 3907, 4004
PvuII CAGCTG 2 cut(s) 4619, 5135
RseI CAYNNNNRTG 6 cut(s) 243, 840, 1578, 2457, 3228, 4649
SacI GAGCTC 1 cut(s) 4139
SalI GTCGAC 1 cut(s) 4
SapI GCTCTTC 1 cut(s) 1848
ScaI AGTACT 1 cut(s) 5150
SchI GAGTC 8 cut(s) 765, 770, 1564, 1763, 3214, 4360, 5003, 5245
ScrFI CCNGG 9 cut(s) 897, 1404, 2342, 2612, 2760, 4392, 4632, 4661, 5354
SduI GDGCHC 7 cut(s) 305, 371, 1935, 1966, 3622, 4139, 4554
SfcI CTRYAG 8 cut(s) 267, 1549, 1875, 2022, 3762, 3869, 4620, 4784
SfuI TTCGAA 1 cut(s) 1015
SinI GGWCC 4 cut(s) 2392, 3484, 3588, 3915
SmiMI CAYNNNNRTG 6 cut(s) 243, 840, 1578, 2457, 3228, 4649
SmlI CTYRAG 8 cut(s) 405, 696, 1131, 1414, 1749, 3061, 3370, 3674
SmoI CTYRAG 8 cut(s) 405, 696, 1131, 1414, 1749, 3061, 3370, 3674
SpeI ACTAGT 2 cut(s) 2156, 3647
SphI GCATGC 1 cut(s) 3814
SseBI AGGCCT 2 cut(s) 77, 3253
SsiI CCGC 8 cut(s) 1655, 2027, 2400, 3653, 4909, 4946, 4998, 5127
SspI AATATT 2 cut(s) 4567, 4579
SstI GAGCTC 1 cut(s) 4139
StuI AGGCCT 2 cut(s) 77, 3253
StyD4I CCNGG 9 cut(s) 895, 1402, 2340, 2610, 2758, 4390, 4630, 4659, 5352
StyI CCWWGG 3 cut(s) 3154, 3636, 5090
TatI WGTACW 4 cut(s) 1022, 2884, 3670, 5148
TseFI GTSAC 1 cut(s) 2448
Tsp45I GTSAC 1 cut(s) 2448
TspGWI ACGGA 2 cut(s) 320, 1665
VneI GTGCAC 1 cut(s) 301
VpaK11BI GGWCC 4 cut(s) 2392, 3484, 3588, 3915
VspI ATTAAT 1 cut(s) 3191
XagI CCTNNNNNAGG 1 cut(s) 5357
XapI RAATTY 8 cut(s) 1157, 1499, 1940, 2048, 3858, 4027, 4349, 5321
XbaI TCTAGA 5 cut(s) 1211, 2102, 2438, 3403, 4330
XceI RCATGY 3 cut(s) 1973, 3814, 3905
XcmI CCANNNNNNNNNTGG 2 cut(s) 551, 4982
XmaJI CCTAGG 1 cut(s) 5090
XmiI GTMKAC 3 cut(s) 5, 1476, 2642
XmnI GAANNNNTTC 2 cut(s) 3072, 4102
ZraI GACGTC 1 cut(s) 1599
ZrmI AGTACT 1 cut(s) 5150
Zsp2I ATGCAT 1 cut(s) 2280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.