MD11G1029700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0002394
Physical Location & Seq
Forward (+)
2603474 .. 2611889
8416 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1029700.v1.1.491

Sequence Viewer

Length: 5613 bp
ATGGAGAAGCTGTTGGTGCAGATCTTCGAGCGGAAGAAATGGATTGTTAACCAAGCAAAGCAGCAGACTTTACTCTTCGAGCAACACCTCGCCTCCAAATGCCTAATAGACGGAATCGCCCCTCCTCCCTGGCTTTGCTCCTCCACCGACCCCAACAATGTGTTGAAAAAACAAGAGCTGAATTCCGGAGTTCCACTCCGTGGCGCGCTGCCTGTGATCCCTTTTAGTAGTCACTGTTCTGTGTTTGACAAGCCAGCTCCCACAACTCACAGTGGGGAGCTACCAAATGGTTTGTGCACTGAACTTCATGGTTTGGACAAAGGTTTTGGTGCTGGAGATGAGGTGTCAGTTTTGCCACGGTGCTGCGTAGGTAATGCTTGGCGTGTTTCCAAAAGCGTTCCTCAAGATGTGAGGGAAGAGGATCCTAGTGTTACATCTCCAGAAGATGTGAGGGATGCAAGAATCTTAGACATTTACCATGATCCAGCTCTATCAGCAGCTAGAGGTGCAGGAGATGGGGTATCAATCTTGCCACAATGCCCTGTTAGTAATGCCGCATGTTCCTCCAACGGTGTTCCTCAAGATCCGAGAAAAGAGGATCTGAGTAATACATCTCCTGAAGATCACAGGGATGCAAGAACCTCTGACATTTACCATGACCCAGCTCTATCACTGGCTAGAGTTCAGAGATCCAAATCCAGACAAAAAGCTTTGGAAATTCGCAACAGTGCCAAAAAATGCTTTTCACTGGACAAGAACAATGGTATTGGGTATGCTAGTGGAACAATTGGGTGTGCGATATCTTCCCTACAGTCTGACCATGTTGATGAACTGAACTTATTCAAACCTCCTGAGACAAGTGATTATGCTTGTGAAGTGGAAGAAGCAAAAATAGGCGAGTTTCAAAGCAAGGAAAGAGGTACTACCATCTCCTCTGGTAGAATTACAAGATCTAGAAGCTCTGGTCAACAGGAGAGGTCTTTGAATGTCAGTGGCTCGGTTTGTATTGACCAAAAAAGTGGCGGTGCACTTGCAGATTCTATTGGCATATTACCTCAGCAGTCCAACCATGTAAATAAACAGTTGGAATTGGTTAATTTCTCTCATATCACTAATGATTGTCGCCCAGAGAATGAAGCAAAAGTAGGTGATTATTTTATCAAGAAAAAGGGAAGCAGCATGTACTCTGGGAGAACAACAAGATCTAGAAGTGCTAGTCAAGAACCTAATCGTCTTGATGAAATGTCCAAGTTGGAGCATTTATCTAACAGCATAAGTGAAGATGGTGTCAGAAATTCATTGCAACAACCGAAACATGTTGATGAAATGGTGGAAGTGGTGAAATCTTGTGATGTTATTGATGGAAGCTGTGAAGCGAAAACAAAAGTGGGGGAGTGCTGGGGCAAGGGAACCAGTGATTACAGTGGCAGAATAACAAGATCCAGAAGTTCTTCCCGAAACCCAAATGATGTTAACAATTCTCCGAAGATAGATACTTCCTGTGTTTCTGTGAAGGGTGATGGCAGTATAATCCATCATTCCAGCGGTACGTCAACACAGCCACCTCAGCCTTTAAGTTGGACTACAGGAGATCCTGATTTCCATGGTGCACATGGGTCTCAAGTGTCCATTTTAAGGTCTCTACCTGCTCAAAGTAATCTTGAGTTTTGTGCAGTAAATGGGGAAAAGCATTCAAGTAAGGAGGATTCTTCTGGGGAGTATGTCTTCAGAAGTACTGGGTCACAAGCTGTGTGTAGGACTGAAAAAATAGTGAAAGGTGCCATCGCCAATACATCCAGTCAAAGAATTAATCAGTCAACATATGCTGCATCTAAAAAGTCTCATGATTCACAGAGAACTCAAATATCTGCTGGAAGGTCTGTTCCAATTCAATGTGATCTAGATCTGTGGGCAGAGAATTCAACAGATTCTTCAGACAAGGAAAATGATGCAGATCACTATGCTGGTAGAAAGACAGGTAACAACAACAACAGCAAGCATTTGTCAGAGAAAATGACTCGTGGGTCTGCAGGAATCAGGCACCAGGATGTGTCAAGTACTATGGTAAAAGAGGTACCTTGTACGCAAACCAGTGAATTTGTTAAAGCTGCAACTGGTGGAGAAACTGAAATTGATCCTGATGTATTGGTTGAAGTTTCTGGATCAAAATTGGATGGTACTGGGCTTAAAGTTGGAGTGGAAGTTTTAGCCTCAAGGCAGCCAGCTGATTGTAATGTGTTTGTGAATCCCAAGCAACTTAATTTTGATGATGTGGAAGAGTCCTGTAGGAATGGATGTTATACTCCTCTTAAAGAGGGGATGCAGCGAAGGTCATCAGAGAAAAGTTCTCTTTCCGTGATGCACACTGAAGATATACGGGACGAAGGAATGACTGTTGATTACCAAGAAAACTGTAACTCATCCTTGCCGATGAATTTTCTCGGGGATCCAGAAGTTTCAGTCAAAGGAAAGGACCTCCAGAGTGCTTTATCTGAATCCCCTGCAGAGGATTCTAAAAGTATCTCTAATGGAAATGCTGTCATATCAGTAAAAGAGGCATCCTATGATCATAATGATGGAGGTACTAGTACTCTGCTAGAAAGTGGCAATAGAGAGTCTCTGTGTGGAAGCACAGGAAGCGTAGAGCACAGTCCAAAGGCCTCACTAGAATCTCAAGGCCCACTTTCTCGGGAGGATGTTGCAAAATCAATTCTTAGTAGAAGTCCAGTTGAAGAAAAGCCTCGACATGAGGACCAAGACATGATAGAGAGCTCTGAATCTTCACCCAAGGCACAGATTAAAGAGGGTGAAACTAGTGATGAACTGAACGTAGTCAAACCTCCTGATACAAGTGCTTGCGAAGTGGAAGAGGCAAAAATAGGTGATTTTCAAAGCAAGGAAAGAGGCACTGCTATCTCCTCTAGTAGAACTGCAAGATCTAGAAGCTCTGGCCAACAGGAGAGCTCTTTGAATGTCAGTGATTCAGCCAGTAATGCCAGAAAAACTGGCGGTACACTTGCAGGTTCTATTGGCATATCACCTCGGCTGTCTAACCATGCAAATCAACCCTTGGAATCGGTTAATTTTTCTCATATCACTAATGAAAGTTGCCAAAAGAAGGAAGCAAAGGCAAGAGATCACTTTCTTTTGGAGGGTTCATGCCCTCAGCATAAACGAAGAAAGATTGGGCATAAAACAGTTGATTGCTTGTCTGCTTCCTTGGACTTGAGAGAACATGGCTTTTACACTGTCAGTAGAGATTCTGCGTGTGGGAACTTGGGAAGTGTAGAACATCGTCCAAAGGCCATGCTAGAATCTCAAGGACTTTCAGTTTCCCAGGAGGATGTTGTGAAATCAAATGTCAGCAGAATCTCAGTCGAAGAAACACATCAAAATGAGGATCACCACATGATAGATTGGTCTGAATCTTCACCTAAGGCACAGATGACAGAGGGTAGAATCAGCTTGGGATGCGGGAATACAAGTGGGAATGCACCTTTCACTTTCATGGATAAAGAATTTGAAGAATCAATCTTAAGTTTGATGAAGCAGACTGGTGGCCGGTCTCAAAATTCTTTGATGGAGGAAACAGGAGTAGCACATCCGCCAAGCATTATCGTTGATTCACGAAGTCAATGCATAGAAAAGAACACTGTTTCATTAACTCTTGAGAATCATCTTACAATAGGACATGCTGAACATATGACTTGTGCTCGAAGAGAAATGCAGGAAATGAGATTTGATCTTGAAGGGACCAGTAATTTTGGATCTCCACGTAGTCAATCCTTGGATTTGATTGGTTCCGATGATACAAAGCCTGAGCTTGAGGGGTTCGTTATGCAAACAGATGATGAACCAACAAGCATTGCTGGAAAGGGAGTTGGCTTTGACGAGTGGAACCTTCCAAGCACTACAATTGAACATGCCAGCATTCTGGAGCAGCTGTGCCAATCTGCTTGCATGCAAACTCCAGTTGCATGCTCTTCTGCTTCATATAAGTTGCATAATATTCCAAATCTCTACCAGTCTGTTCCAACTGGGCTTCTAGAGGGTGTGGATATGAGGACCATGAATGATGCTGTCAAGCAATTAAGGGATGGTCATAGTTGCTCGAGTGAGGAAGTTGGCCAGGCGTTTTATGCAAGGTCCTATTCTGATTGCCTATTAAATCATAGTGGTCAATCTAGTTGGGATACTAAGAAACCTTATCTGTCTCCAGTTGGGAAACTTTGGGATATAACTGGTTCAAGGACTAGCAGTTCAAGGAAGCGAGGAAGCTTAAATCCGGAGCTTCCCTGCATCAGTGAAGAAAATGAGAATGCAGATGAGGTAGCTGATATTTTCCAAGATGGCATTGTTTCAGATGCATTAAATAGCTCAATACAAAGAGTACCCCTTGCTGACATTACTGAGATTCCAAACCCTCCTGCATCAGTTTCTAAAGCTGAACCATATGCAGATAGACTTAGTCTAGATTCCGTGAACACTGAAATCAGCTTTACAGGCACTCATAAGAGCTTCAAACAGAGGCTTCGAATCCAAAACAGCAGCAAGAGAAGATATAACAATAAGGAGACAAACGATATTAAGAGGACCACTGGATCACTTCATAACAGGTTCAGTAAACCAAAATTATCTGGAAAAACCAGTTTGAGAAAAGGTGGTCCAAGTTTGTCAGAGCAGGAGCCGAAACGTAACAATATCGTGTCTAGCATGACTTCTTTCATTCCACTTGTTCAACAGAAACAAGCAGCTGCAGTTATAACAGGAAAGAGGGACATCAAAGTGAAGGCCCTGGAGGCAGCTGAAGCTACAAAGCGTCTTGCAGAAAAGAAAGAGAATGAACGCAAGATGAAGAAGGAAGCCTTGAAACTTCACCGGTCAAGAATGGAGCAGGAGAATATGAAGCAGTTGGAGCTGCAGAAAAAAAAGAAAGAAGAAGAGAGGAAGAAAAAAGAAGCTGATATGGCATCAAAGAAGAGACAAAGGGAAGAGGAAGAAAGGAAGGAGAAGGAAAGAAAAAGAATGCGTGTTGAAGCCCGTAGACAGCAGAGAGAACATGAATACAAGTTACCTGCTGAAAAAGTAGAGAATGAAATGAAAAGCCAGGCCATTGATGGAAGAGGCCCTGGCGGTAAGAAATCTAAGGATGAAATACCACATAAGAAAATGGAGGCAGAAAGGGGATTTGACAATTCCAGAAATATTTCGGAGACTGAGCCTAGTACTTCCAGGATTTCAATAAGCAATGCCGGAAGAGAAAGTATTGTCCACGAAGAGTTCCATGAGGCCTTGAGTAATTATGGGTATAAAGCAGAGGTACCGAGCGATTTAGACAAAGAAATGGAGAATTCAATTGGCATTACAGGTCAAGAGCAGTCTTATGACATCTCTCCATACAAAGAATCAGATGATGAAAATGACGATGATGACAATGTCATACCAAATAGGAAATTTATTCCTTCATGGTCAAGTAAAAACTGCCTGGCTTTTGCCGTTTCTTGCCAGAATAGAGCAGACCCAGCGACAATCTTCCCCCCAGAAAGCTTTTGCTGCATCTCTGAAGTCCTCTTGCCCCGAAACCATCAATTAAACAACAGCAGAATGCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
INCENP_ARK-bind

Protein Analysis

1871

Amino Acids

205.44

Weight (kDa)

5.92

Isoelectric Point (pI)

54.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
INCENP_ARK-bind PF03941 1803 - 1855 6.9e-07 Inner centromere protein, ARK binding region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 4763
AasI GACNNNNNNGTC 1 cut(s) 2023
Acc36I ACCTGC 3 cut(s) 1654, 3011, 5083
Acc65I GGTACC 2 cut(s) 2074, 5320
AccB1I GGYRCC 4 cut(s) 1778, 2040, 2074, 5320
AccB7I CCANNNNNTGG 1 cut(s) 200
AccBSI CCGCTC 1 cut(s) 31
AccI GTMKAC 1 cut(s) 5044
AccII CGCG 1 cut(s) 206
AccIII TCCGGA 2 cut(s) 185, 4285
AciI CCGC 8 cut(s) 31, 555, 1023, 1545, 3009, 3474, 3605, 5133
AcoI YGGCCR 3 cut(s) 2950, 3559, 4126
AcuI CTGAAG 6 cut(s) 639, 1711, 1917, 2388, 4827, 5583
AdeI CACNNNGTG 1 cut(s) 200
AfiI CCNNNNNNNGG 4 cut(s) 200, 1635, 2506, 3118
AflII CTTAAG 1 cut(s) 3535
AflIII ACRYGT 1 cut(s) 1315
AgeI ACCGGT 1 cut(s) 4878
AhlI ACTAGT 2 cut(s) 2585, 2813
AjnI CCWGG 9 cut(s) 128, 2043, 3336, 4128, 4794, 5106, 5128, 5231, 5484
AloI GAACNNNNNNTCC 4 cut(s) 1866, 1898, 4243, 4275
Alw21I GWGCWC 7 cut(s) 299, 1030, 1612, 2649, 2774, 2966, 3715
Alw44I GTGCAC 3 cut(s) 295, 1026, 1608
Ama87I CYCGRG 3 cut(s) 2441, 2688, 4111
Aor13HI TCCGGA 2 cut(s) 185, 4285
ApaLI GTGCAC 3 cut(s) 295, 1026, 1608
ArsI GACNNNNNNTTYG 2 cut(s) 308, 340
AseI ATTAAT 1 cut(s) 1809
AsiGI ACCGGT 1 cut(s) 4878
Asp700I GAANNNNTTC 3 cut(s) 839, 1450, 5206
Asp718I GGTACC 2 cut(s) 2074, 5320
AspLEI GCGC 2 cut(s) 206, 208
AsuII TTCGAA 1 cut(s) 4534
AvaI CYCGRG 3 cut(s) 2441, 2688, 4111
AvaII GGWCC 7 cut(s) 2473, 2752, 3753, 4065, 4146, 4593, 4664
AxyI CCTNAGG 1 cut(s) 3435
BaeGI GKGCMC 3 cut(s) 299, 1030, 1612
BalI TGGCCA 2 cut(s) 2952, 4128
BamHI GGATCC 2 cut(s) 421, 2446
BanI GGYRCC 4 cut(s) 1778, 2040, 2074, 5320
BanII GRGCYC 2 cut(s) 2774, 2966
BauI CACGAG 1 cut(s) 2019
BbsI GAAGAC 1 cut(s) 1717
Bbv12I GWGCWC 7 cut(s) 299, 1030, 1612, 2649, 2774, 2966, 3715
BbvCI CCTCAGC 3 cut(s) 1056, 1566, 3165
BceAI ACGGC 1 cut(s) 5480
BcgI CGANNNNNNTGC 2 cut(s) 3618, 3652
BciT130I CCWGG 9 cut(s) 130, 2045, 3338, 4130, 4796, 5108, 5130, 5233, 5486
BciVI GTATCC 1 cut(s) 4186
BclI TGATCA 1 cut(s) 2566
BcuI ACTAGT 2 cut(s) 2585, 2813
BfmI CTRYAG 7 cut(s) 809, 1584, 2028, 2284, 2502, 4755, 4919
BfrI CTTAAG 1 cut(s) 3535
BfuAI ACCTGC 3 cut(s) 1654, 3011, 5083
BfuI GTATCC 1 cut(s) 4186
BglI GCCNNNNNGGC 1 cut(s) 4799
BglII AGATCT 5 cut(s) 21, 950, 1202, 1903, 2936
BmcAI AGTACT 4 cut(s) 1735, 2059, 2590, 5227
Bme1390I CCNGG 9 cut(s) 130, 2045, 3338, 4130, 4796, 5108, 5130, 5233, 5486
Bme18I GGWCC 7 cut(s) 2473, 2752, 3753, 4065, 4146, 4593, 4664
BmeT110I CYCGRG 3 cut(s) 2441, 2688, 4111
BmrFI CCNGG 9 cut(s) 130, 2045, 3338, 4130, 4796, 5108, 5130, 5233, 5486
BmrI ACTGGG 3 cut(s) 1746, 2190, 4047
BmuI ACTGGG 3 cut(s) 1746, 2190, 4047
BpiI GAAGAC 1 cut(s) 1717
BplI GAGNNNNNCTC 2 cut(s) 180, 212
BpmI CTGGAG 7 cut(s) 354, 423, 2462, 3954, 3956, 4200, 4817
Bpu10I CCTNAGC 4 cut(s) 1056, 1566, 3165, 3819
Bpu14I TTCGAA 1 cut(s) 4534
BsaAI YACGTR 1 cut(s) 3776
BsaBI GATNNNNATC 3 cut(s) 694, 1902, 1953
BsaI GGTCTC 3 cut(s) 1623, 1644, 3570
BsaWI WCCGGW 3 cut(s) 185, 4285, 4878
Bsc4I CCNNNNNNNGG 4 cut(s) 200, 1635, 2506, 3118
Bse118I RCCGGY 2 cut(s) 3561, 4878
Bse21I CCTNAGG 1 cut(s) 3435
Bse3DI GCAATG 3 cut(s) 1298, 3864, 5254
Bse8I GATNNNNATC 3 cut(s) 694, 1902, 1953
BseAI TCCGGA 2 cut(s) 185, 4285
BseBI CCWGG 9 cut(s) 130, 2045, 3338, 4130, 4796, 5108, 5130, 5233, 5486
BseJI GATNNNNATC 3 cut(s) 694, 1902, 1953
BseLI CCNNNNNNNGG 4 cut(s) 200, 1635, 2506, 3118
BseMI GCAATG 3 cut(s) 1298, 3864, 5254
BseMII CTCAG 9 cut(s) 593, 843, 1070, 1580, 3179, 3387, 3810, 4401, 5208
BsePI GCGCGC 1 cut(s) 204
BseRI GAGGAG 5 cut(s) 114, 130, 922, 2295, 2908
BseSI GKGCMC 3 cut(s) 299, 1030, 1612
BseYI CCCAGC 3 cut(s) 661, 1398, 5521
BsgI GTGCAG 3 cut(s) 38, 528, 1692
Bsh1236I CGCG 1 cut(s) 206
BshNI GGYRCC 4 cut(s) 1778, 2040, 2074, 5320
BshTI ACCGGT 1 cut(s) 4878
BsiHKAI GWGCWC 7 cut(s) 299, 1030, 1612, 2649, 2774, 2966, 3715
BsiHKCI CYCGRG 3 cut(s) 2441, 2688, 4111
BsiSI CCGG 5 cut(s) 186, 3562, 4286, 4879, 5253
BslFI GGGAC 3 cut(s) 2393, 3766, 4790
BslI CCNNNNNNNGG 4 cut(s) 200, 1635, 2506, 3118
BsmFI GGGAC 3 cut(s) 2393, 3766, 4790
BsmI GAATGC 6 cut(s) 1690, 3496, 3930, 4324, 5031, 5610
Bso31I GGTCTC 3 cut(s) 1623, 1644, 3570
BsoBI CYCGRG 3 cut(s) 2441, 2688, 4111
Bsp119I TTCGAA 1 cut(s) 4534
Bsp1286I GDGCHC 7 cut(s) 299, 1030, 1612, 2649, 2774, 2966, 3715
Bsp13I TCCGGA 2 cut(s) 185, 4285
Bsp19I CCATGG 1 cut(s) 1603
BspACI CCGC 8 cut(s) 31, 555, 1023, 1545, 3009, 3474, 3605, 5133
BspCNI CTCAG 9 cut(s) 594, 844, 1069, 1579, 3178, 3386, 3811, 4402, 5209
BspEI TCCGGA 2 cut(s) 185, 4285
BspFNI CGCG 1 cut(s) 206
BspHI TCATGA 1 cut(s) 1843
BspMAI CTGCAG 4 cut(s) 2032, 2506, 4759, 4923
BspMI ACCTGC 3 cut(s) 1654, 3011, 5083
BspQI GCTCTTC 1 cut(s) 3988
BspT104I TTCGAA 1 cut(s) 4534
BspT107I GGYRCC 4 cut(s) 1778, 2040, 2074, 5320
BspTI CTTAAG 1 cut(s) 3535
BspTNI GGTCTC 3 cut(s) 1623, 1644, 3570
BsrBI CCGCTC 1 cut(s) 31
BsrDI GCAATG 3 cut(s) 1298, 3864, 5254
BsrFI RCCGGY 2 cut(s) 3561, 4878
BssAI RCCGGY 2 cut(s) 3561, 4878
BssHII GCGCGC 1 cut(s) 204
BssSI CACGAG 1 cut(s) 2019
BssT1I CCWWGG 5 cut(s) 1603, 2787, 3069, 3219, 3786
Bst2BI CACGAG 1 cut(s) 2019
Bst2UI CCWGG 9 cut(s) 130, 2045, 3338, 4130, 4796, 5108, 5130, 5233, 5486
BstAFI CTTAAG 1 cut(s) 3535
BstBAI YACGTR 1 cut(s) 3776
BstBI TTCGAA 1 cut(s) 4534
BstC8I GCNNGC 9 cut(s) 206, 255, 1997, 2223, 2857, 3928, 3958, 3962, 3979
BstDSI CCRYGG 3 cut(s) 199, 356, 1603
BstFNI CGCG 1 cut(s) 206
BstHHI GCGC 2 cut(s) 206, 208
BstNI CCWGG 9 cut(s) 130, 2045, 3338, 4130, 4796, 5108, 5130, 5233, 5486
BstNSI RCATGY 7 cut(s) 561, 1183, 1319, 3695, 3926, 3964, 3981
BstSCI CCNGG 9 cut(s) 128, 2043, 3336, 4128, 4794, 5106, 5128, 5231, 5484
BstSFI CTRYAG 7 cut(s) 809, 1584, 2028, 2284, 2502, 4755, 4919
BstSLI GKGCMC 3 cut(s) 299, 1030, 1612
BstUI CGCG 1 cut(s) 206
BstV2I GAAGAC 1 cut(s) 1717
BstXI CCANNNNNNTGG 2 cut(s) 1019, 3934
Bsu36I CCTNAGG 1 cut(s) 3435
BsuI GTATCC 1 cut(s) 4186
BtgI CCRYGG 3 cut(s) 199, 356, 1603
BtgZI GCGATG 1 cut(s) 1768
BtsI GCAGTG 1 cut(s) 2907
BveI ACCTGC 3 cut(s) 1654, 3011, 5083
Cac8I GCNNGC 9 cut(s) 206, 255, 1997, 2223, 2857, 3928, 3958, 3962, 3979
CciI TCATGA 1 cut(s) 1843
CfoI GCGC 2 cut(s) 206, 208
Cfr10I RCCGGY 2 cut(s) 3561, 4878
CseI GACGC 1 cut(s) 4808
CspAI ACCGGT 1 cut(s) 4878
DraIII CACNNNGTG 1 cut(s) 200
DrdI GACNNNNNNGTC 1 cut(s) 2023
DseDI GACNNNNNNGTC 1 cut(s) 2023
EaeI YGGCCR 3 cut(s) 2950, 3559, 4126
EciI GGCGGA 1 cut(s) 3594
Ecl136II GAGCTC 2 cut(s) 2772, 2964
Eco130I CCWWGG 5 cut(s) 1603, 2787, 3069, 3219, 3786
Eco147I AGGCCT 2 cut(s) 2660, 5291
Eco24I GRGCYC 2 cut(s) 2774, 2966
Eco31I GGTCTC 3 cut(s) 1623, 1644, 3570
Eco32I GATATC 1 cut(s) 801
Eco47I GGWCC 7 cut(s) 2473, 2752, 3753, 4065, 4146, 4593, 4664
Eco53kI GAGCTC 2 cut(s) 2772, 2964
Eco57I CTGAAG 6 cut(s) 639, 1711, 1917, 2388, 4827, 5583
Eco81I CCTNAGG 1 cut(s) 3435
Eco88I CYCGRG 3 cut(s) 2441, 2688, 4111
EcoICRI GAGCTC 2 cut(s) 2772, 2964
EcoO109I RGGNCCY 4 cut(s) 2473, 4146, 4792, 5126
EcoRI GAATTC 3 cut(s) 181, 1918, 5350
EcoRII CCWGG 9 cut(s) 128, 2043, 3336, 4128, 4794, 5106, 5128, 5231, 5484
EcoRV GATATC 1 cut(s) 801
EcoT14I CCWWGG 5 cut(s) 1603, 2787, 3069, 3219, 3786
EcoT22I ATGCAT 2 cut(s) 3641, 4369
EcoT38I GRGCYC 2 cut(s) 2774, 2966
ErhI CCWWGG 5 cut(s) 1603, 2787, 3069, 3219, 3786
FalI AAGNNNNNCTT 4 cut(s) 2667, 2699, 4850, 4882
FaqI GGGAC 3 cut(s) 2393, 3766, 4790
FauI CCCGC 1 cut(s) 3467
FauNDI CATATG 3 cut(s) 1822, 3702, 4453
FbaI TGATCA 1 cut(s) 2566
FblI GTMKAC 1 cut(s) 5044
FriOI GRGCYC 2 cut(s) 2774, 2966
GlaI GCGC 2 cut(s) 205, 207
GsaI CCCAGC 3 cut(s) 665, 1402, 5525
GsuI CTGGAG 7 cut(s) 354, 423, 2462, 3954, 3956, 4200, 4817
HapII CCGG 5 cut(s) 186, 3562, 4286, 4879, 5253
HgaI GACGC 1 cut(s) 4808
HhaI GCGC 2 cut(s) 206, 208
Hin6I GCGC 2 cut(s) 204, 206
HinP1I GCGC 2 cut(s) 204, 206
HincII GTYRAC 5 cut(s) 49, 968, 1474, 1554, 1818
HindII GTYRAC 5 cut(s) 49, 968, 1474, 1554, 1818
HindIII AAGCTT 3 cut(s) 708, 4276, 5545
HpaI GTTAAC 2 cut(s) 49, 1474
HpaII CCGG 5 cut(s) 186, 3562, 4286, 4879, 5253
HpyCH4IV ACGT 4 cut(s) 1550, 2829, 3775, 4693
HpySE526I ACGT 4 cut(s) 1550, 2829, 3775, 4693
HspAI GCGC 2 cut(s) 204, 206
Kpn2I TCCGGA 2 cut(s) 185, 4285
KpnI GGTACC 2 cut(s) 2078, 5324
Ksp22I TGATCA 1 cut(s) 2566
KspAI GTTAAC 2 cut(s) 49, 1474
LguI GCTCTTC 1 cut(s) 3988
LmnI GCTCC 9 cut(s) 143, 262, 277, 1255, 3937, 4288, 4684, 4891, 4915
MaeII ACGT 4 cut(s) 1550, 2829, 3775, 4693
MaeIII GTNAC 7 cut(s) 230, 430, 1740, 1979, 2414, 4694, 5070
MbiI CCGCTC 1 cut(s) 31
MfeI CAATTG 3 cut(s) 786, 3915, 5355
MhlI GDGCHC 7 cut(s) 299, 1030, 1612, 2649, 2774, 2966, 3715
MlsI TGGCCA 2 cut(s) 2952, 4128
MluNI TGGCCA 2 cut(s) 2952, 4128
MlyI GAGTC 3 cut(s) 2011, 2288, 2624
MmeI TCCRAC 8 cut(s) 591, 1065, 1089, 1233, 1559, 2173, 4058, 4893
Mox20I TGGCCA 2 cut(s) 2952, 4128
Mph1103I ATGCAT 2 cut(s) 3641, 4369
MroI TCCGGA 2 cut(s) 185, 4285
MroXI GAANNNNTTC 3 cut(s) 839, 1450, 5206
MscI TGGCCA 2 cut(s) 2952, 4128
MslI CAYNNNNRTG 8 cut(s) 630, 825, 1320, 2046, 2574, 3393, 3506, 4784
Msp20I TGGCCA 2 cut(s) 2952, 4128
MspA1I CMGCKG 5 cut(s) 1545, 2225, 3943, 4754, 4805
MspCI CTTAAG 1 cut(s) 3535
MspI CCGG 5 cut(s) 186, 3562, 4286, 4879, 5253
MspR9I CCNGG 9 cut(s) 130, 2045, 3338, 4130, 4796, 5108, 5130, 5233, 5486
MunI CAATTG 3 cut(s) 786, 3915, 5355
Mva1269I GAATGC 6 cut(s) 1690, 3496, 3930, 4324, 5031, 5610
MvaI CCWGG 9 cut(s) 130, 2045, 3338, 4130, 4796, 5108, 5130, 5233, 5486
MvnI CGCG 1 cut(s) 206
NcoI CCATGG 1 cut(s) 1603
NdeI CATATG 3 cut(s) 1822, 3702, 4453
NmeAIII GCCGAG 1 cut(s) 3022
NmuCI GTSAC 2 cut(s) 230, 1740
NsiI ATGCAT 2 cut(s) 3641, 4369
NspI RCATGY 7 cut(s) 561, 1183, 1319, 3695, 3926, 3964, 3981
NspV TTCGAA 1 cut(s) 4534
PaeI GCATGC 2 cut(s) 3964, 3981
PaeR7I CTCGAG 1 cut(s) 4111
PagI TCATGA 1 cut(s) 1843
PauI GCGCGC 1 cut(s) 204
PceI AGGCCT 2 cut(s) 2660, 5291
PciI ACATGT 1 cut(s) 1315
PciSI GCTCTTC 1 cut(s) 3988
PctI GAATGC 6 cut(s) 1690, 3496, 3930, 4324, 5031, 5610
PdmI GAANNNNTTC 3 cut(s) 839, 1450, 5206
PflFI GACNNNGTC 2 cut(s) 4467, 5435
PflMI CCANNNNNTGG 1 cut(s) 200
PfoI TCCNGGA 1 cut(s) 5231
PinAI ACCGGT 1 cut(s) 4878
PleI GAGTC 3 cut(s) 2011, 2287, 2623
PpsI GAGTC 3 cut(s) 2011, 2287, 2623
Ppu21I YACGTR 1 cut(s) 3776
PpuMI RGGWCCY 2 cut(s) 2473, 4146
PscI ACATGT 1 cut(s) 1315
PshBI ATTAAT 1 cut(s) 1809
PsiI TTATAA 1 cut(s) 4763
Psp124BI GAGCTC 2 cut(s) 2774, 2966
Psp5II RGGWCCY 2 cut(s) 2473, 4146
Psp6I CCWGG 9 cut(s) 128, 2043, 3336, 4128, 4794, 5106, 5128, 5231, 5484
PspFI CCCAGC 3 cut(s) 661, 1398, 5521
PspGI CCWGG 9 cut(s) 128, 2043, 3336, 4128, 4794, 5106, 5128, 5231, 5484
PspPPI RGGWCCY 2 cut(s) 2473, 4146
PspXI VCTCGAGB 1 cut(s) 4111
PsrI GAACNNNNNNTAC 2 cut(s) 220, 252
PstI CTGCAG 4 cut(s) 2032, 2506, 4759, 4923
PsyI GACNNNGTC 2 cut(s) 4467, 5435
PteI GCGCGC 1 cut(s) 204
PvuII CAGCTG 4 cut(s) 2225, 3943, 4754, 4805
RseI CAYNNNNRTG 8 cut(s) 630, 825, 1320, 2046, 2574, 3393, 3506, 4784
SacI GAGCTC 2 cut(s) 2774, 2966
SapI GCTCTTC 1 cut(s) 3988
ScaI AGTACT 4 cut(s) 1735, 2059, 2590, 5227
SchI GAGTC 3 cut(s) 2011, 2288, 2624
ScrFI CCNGG 9 cut(s) 130, 2045, 3338, 4130, 4796, 5108, 5130, 5233, 5486
SduI GDGCHC 7 cut(s) 299, 1030, 1612, 2649, 2774, 2966, 3715
SfcI CTRYAG 7 cut(s) 809, 1584, 2028, 2284, 2502, 4755, 4919
Sfr274I CTCGAG 1 cut(s) 4111
SfuI TTCGAA 1 cut(s) 4534
SinI GGWCC 7 cut(s) 2473, 2752, 3753, 4065, 4146, 4593, 4664
SlaI CTCGAG 1 cut(s) 4111
SmiMI CAYNNNNRTG 8 cut(s) 630, 825, 1320, 2046, 2574, 3393, 3506, 4784
SpeI ACTAGT 2 cut(s) 2585, 2813
SphI GCATGC 2 cut(s) 3964, 3981
SseBI AGGCCT 2 cut(s) 2660, 5291
SsiI CCGC 8 cut(s) 31, 555, 1023, 1545, 3009, 3474, 3605, 5133
SspI AATATT 2 cut(s) 4009, 5206
SstI GAGCTC 2 cut(s) 2774, 2966
StuI AGGCCT 2 cut(s) 2660, 5291
StyD4I CCNGG 9 cut(s) 128, 2043, 3336, 4128, 4794, 5106, 5128, 5231, 5484
StyI CCWWGG 5 cut(s) 1603, 2787, 3069, 3219, 3786
TaiI ACGT 4 cut(s) 1553, 2832, 3778, 4696
TaqI TCGA 7 cut(s) 27, 78, 2743, 3378, 3715, 4112, 4534
TatI WGTACW 5 cut(s) 1182, 1733, 2057, 2588, 5225
TauI GCSGC 1 cut(s) 557
TseFI GTSAC 2 cut(s) 230, 1740
Tsp45I GTSAC 2 cut(s) 230, 1740
TspGWI ACGGA 4 cut(s) 126, 188, 2344, 4468
Tth111I GACNNNGTC 2 cut(s) 4467, 5435
Van91I CCANNNNNTGG 1 cut(s) 200
Vha464I CTTAAG 1 cut(s) 3535
VneI GTGCAC 3 cut(s) 295, 1026, 1608
VpaK11BI GGWCC 7 cut(s) 2473, 2752, 3753, 4065, 4146, 4593, 4664
VspI ATTAAT 1 cut(s) 1809
XbaI TCTAGA 6 cut(s) 953, 1205, 1900, 2939, 4045, 4471
XceI RCATGY 7 cut(s) 561, 1183, 1319, 3695, 3926, 3964, 3981
XcmI CCANNNNNNNNNTGG 2 cut(s) 1610, 5114
XhoI CTCGAG 1 cut(s) 4111
XmiI GTMKAC 1 cut(s) 5044
XmnI GAANNNNTTC 3 cut(s) 839, 1450, 5206
ZrmI AGTACT 4 cut(s) 1735, 2059, 2590, 5227
Zsp2I ATGCAT 2 cut(s) 3641, 4369
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.