Rw1G011110

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0002394
Physical Location & Seq
Reverse (-)
25049281 .. 25058212
8932 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G011110.1

Sequence Viewer

Length: 5622 bp
ATGTCGACGACTGAGAAGCACTGGTTGCAGATCTTCGAGGGAAAGCAATGGATTATCAACAAAGCCAAGCAGCAGGCCTTCCTCTTCGACCAAGACCTCGCCTCCAAACTCATCATTGCCGGAATTGCCCCTCCTCCTTCTCTTTTCTCCTCCCACCCCGACGTGTTGAACCATCATGAGCTGATTTCAGAAGTTCCACTCCCTCGCACACGCCGTGTGATCCCTATCACTGGTAGTCATTGCTGTGGGTTCGACAAGCCAGTTGCTACAGCTCACTACGGGCAGCTAACAAATGATTTGTGCACGGAACATTGTGGGGTAAAGAAAGTTTTTGATGTAGGAGGCAATGTATTGGATTTGCCACCGTGCCCAATCAGTAATGGTGGATGTGCCTCCAATGGTGTTCCTCAAGATCATCAAGAAGAGGATCCTAGTGTTACGTCTCCTGAAGATGAGAGGGATGCAAGAGTCTCAGATGCTTACCATGATTCAGCTTTACAACTGGCTAGAGGTGCAGGACAGGAGGTATCAGCTTTGCCACAGTGCCCCATTAGTAATGCTGGGTGTGCTTCTATTGGTGTTCTACAAGACCAGAAAGAAGAGAATTCTGGTGGTACATCTCCTGAAGATCAGAGGAATGCAAAAATGGTACGTATCCCGAAGATCAGTAATGGTGGATGTGCCTCCAATGGTGTCACTCAACATCATAGAGAAGAGGATCCTAGTGTTACGTCTCCTGAAGATGAGAGGGATGAAAGAGTCTCAGACGTTTACCATGACTCAGCTTTAACACCGGCTAGAGGTACAGGAGAGGAGGTATCAATTTTGCCACAGGGCCACATTAGTGATGCTGGGTGTGCCTTTGATGGTGTTTTACAATATCACAAAGAAGAAAATCCTGGTGGTACGTCTCCTGAAGATCAGGGGGATACAAGAATGTTAGACATTTACCTCAACCCAGCTCTTTCACTGGCTAGAGTTCAAAGATCTAAGTCAAGACAAAGGGCTCTGGCAATTCGAAACAGTACTCAAAAAAGCTGTTCACAGGATAAGGGCAATGTTGATGGTTATGCTGGTGAAACTATCAAGTGTCTGATATCTACCCCACAGTCTGAAAATGTCGATGAATTGAATGAGAATCATTCTGAAGTGGAAAAACCAAAAATAGGAGAATTTCAAAGCAAGGAAAAAGGTTCTGACATCTACTCTGGTAGAATTACTAGATCTAGAAGCTCTGGCCATCAGGAGAACTCTTTAGATGTCAGTGGCTCTCCATGTATTTACAAGTCAAGTGATGGTATAGCTGTTGATTCTATTGGTACATTGACACATCTGTCTAACCAAGTAATTCACCCACTGGAATTGGTTAGCTACTGTCATATAACAAATGACGGTTGTCGAGAGGGGGAATTAAAGCTGGGAGACTACTCAAGCAAGAAGCGAACCGTTGAGAAAGATTCTATGTGTGCAAACTTGGAAAGAGATGAACACACTCCAAAGGCTTTACAAGAGTTCAAAGGTCCTTTAATTTCTAAGGATGTTGCTGGGAACTCAATTGTCTGTAGGAGTCCTGTTGAAGAAACACATCTAAATGAGGATCATCACATGGTAAACACGTCCAAATCTTCACCTGAGGCACAGAAGAAAGAGGGCGAAATGGGTTTAGAAGGGGTGGGCAGTAGTCCAAATGCTCTGTTTACTTTTTTGCATGAAGAAAAAGAAGCATCAGTATTCCCATTTCTGATCAAGCAGGCTGCTGGTCATCCTCAAGATTCTTTGCTTGAGGAAACAGGAGTAGCACATCCAACTAGCACTAACATTGATGAAGGAAGCCAATGCTTAAAAGAGAACCCAGTTTCTCTTCTTCCGGACAATTTAAGACTAGAAAATGCTGAAGATTTATCTTGTGCTGGAAAAACCATGCAGGCACAGAGATTTGATTTTGGAGGGCCCAGCAAATTTTTAGAATTGTCATGTGGTGCTCCACATGTTGAATGCTCACAGTCTGAAAATGTTGGTGAATTGAATGATAATCATTCTGAAGTGGAAAAACAAAAGATAGGAGAATTTCAAAGCAAGGAAGAAGGCACTGACATCTACTCTGGTAGAATTACTAGATCTAGAAGCTCTGGCCATCAGGAGAACTCTTTAAATGCCAGTGGCTCGCCCTGCATTGGCAGGACAAGTAGTGGTATAGCAGTTGATATTGATACTGTGGAAGAGTCCTGTTTAAAAGGACTTTCTACTCCTGCTAGTAAGAAGGGAATGCAGGGCAGTTCATCAGTAGAAATGCCTCTTCCCTTCATGCACGGGGAAAAGAAACTAGAAGAAGGAATGAGCATCACTACCCGTGAAAACTGTAACTCATCCTTGGAAATGGATTTTCTGGGCAATTGTGAAGTTTCAGCCAAAGAAATGGATGTCCAGAGCGGTTTAGTTGAAGCCCTTGAAGAGGAGTTCAGTAAATCTAGAAGAGCGTCACATGACAATGTGATCTCATCAGTAAAAGAATCATTTGATGCTTATACGGATGCAGTTCCCAAAACTTTGCTAGAAAGTGGGAGAACTCCGAAACAGAAATGTTCTATGATAGATGATCAAACTCCCTTGCGAGTAGACTGGGATGGATTGGTTTGTAGCCTGGTAAAAGAAGTAAAGGGGTCTAATTTGTCTACTGCTAATGCTGCAACACCAACGGAGTTGGTATCAGAAGACTGTGCAGTGGATTCTAATGCCCTTTGTAATTCTCATAAATCAACAGATGTTGATTTTGCTATGGTGTCTGGGCCTGGTTCTCATAGAATCCTAGATGCAGAGATTCTTGAAGTTGAGAACCCTTATGCTGCTTTTAGGGATGAGCTGAGGAGTAACTTGGCTCAGTCGACTGTAAATGCACACATTTCTCCAGAATATGTACAAAGGAGTGCCAGTGATGACAGAGAGGTAGGTTGTTCAGAATCAACTGAATGCCAGATTGCAGAGAACTCCAAAGGAAGAAGTTTTTGGTATTCAATGGAGGGTACATCACCTACGCTTAAACGAAGAAAGATTGACGATAAAAGAGTTCATGACCTGTCTGCTTCAGTAGCCTTGAGAGAAGAGGTTCTTCACGCTGTCAAAAAAGATTCTATGTGTGCAAACTTGGAAAGAGAAGAACACAGTCCAAAGGCTTTACAACAATCTCAAGGGCGTTGCATTTCTCAGGAGGATGATGGGAAATTAATTGTCAGTAAGAGTCCTGTTGAAGAAACACATCTAAATGAGAATCACATGGTAGAGAGGTCTGAATCTTTACCCGAGGCACAGAAGAAAGAGGGTGGAATGGGTTTGGAAGGGGTGGATAGTAGTCCAAATGCTCCATTCACTTTTTTGCATGAAGAAAAAGAAGCATCAGTTTTCTCAAGATTGATCAAGCAGGCTGCTGGGCATCCTCTAGATTCTTTGCTTGAGGATACAGGAGTAGCAGTTCCAACTGACATTAACATTGATAGAGGAAGCCAATGCTTAAAAGAGGACCCAATTTGTCTTCATCTTCAGGATGATTTAAGAGTAGAAAATGTTGAAGATTGGCCTTGTGCTGGAAGAACCATGCTTGCAAAGAGATCTGATTCTGGAGGGACCAGCAACTTCAAAGAATTGTCAGGTGGTGCTCCACATGTTGAATCCTTGGATTTGACTAGTGCGGATGAAGCAATGCCTGTACTTGAGAGGTTTGTTATAAAGACAGATGATGAACCATGTAGCATTGCTGAAGAGGGAATTAGCTTTGATGAGTGGAACCTTCCAAACACTGCATTAGAGCGTGCCGGTATTCTGGAGCAGCTTTGCAAATCAGCTTGCATGCAAACTCCCGTAGCCTATTCTTTAGCTTCCTATAAGTTGCATAAAGTTCAAAACCTACAGCAATCTGTTCCGACTGGGGTTTTGGAGCATGTAGATCTGAGGACCACCTTTCCTATAAATGATAATGTTAAGCAATTAAAGGATGGTAATAGTTCTTGGAGTGAGGAAGTTGGCCCGGCCTTTTATGGAAGTTCCTATTCTGATTGCCTACCAAATTTTAGTGGTCAATCTAGTTGGGATATTAAGAAACCTTATTCCTCTCCTGTTGGTAATCTTTGGGATAGAATTGCTTCAAGCTCTAGTAGTTCGGGGAAGCGAGTGAGCTCAATTCCAGAGCTTGCCTGTATTAGTGAAGAAAATGAGAATACAGATGAGGTAGCTGATACTTTTCAAGATGGCATTGTTTCAGAAGTGTTAGATTGTTCTCCAAAAAGGCCACCGCTTGCAGACATTACAGAAATCCCTAAGCTTCCTGCTTCAGTTTCTGAAGCTGCAGCCTACACTGATAGATTTAGTCTAGATTCAGTAACCACCGAATTCAGTTTAACGGGGACTCATAAGAGCGTCAAAAAGAAGCCTGGAATCCAAAACAGCAGTAAGAGAAGATATAACAATAAGGAGAACCAGAGTGTATCACGAAAGAGAGCCACCGGATCACTTCAGAATAGGTTCAGCAAGCCAAAGTTTTCTGGAAAAACCAGTTTGAGAAAAGGAGGCCCTAGTTTGTCAGAGGTGGAGCCCAAGCGTAACAATATTGTTTCCAATATTACTTCCTTTATTCCACTTGTGCAACAGAAACAAGCAGCTGCAGTCTTACCAGGGAAGAGGGACATTAAAGTGAAGGCCCTGGAGGCTGCTGAAGCTGCAAAGCGTCTTGCAGAAAGGAAAGACAATGAACGTAAGATGAAGAAAGAAGCTATGAAGGTTGAGCGAGCAAGAGTGGAGCAAGAGAATCTGAGGAAGTTGGAGCTGCAGAGGAAAAGGAAACAAGAGGAGAGGAAAAAAATTGAGGCTGATATGGCAGCAAAGAAGAGAGAAAGGGAAGGGGAAGACAAAAAGGAGAAGCAAAGAAAAAGGATGCGTGTTGAAGAAGGGCGGAGGGAGCAGAGAGAACATGAGGACAAGTTACGTGCGGAAAAAGTAAAGAAAGAAATACAATGCCAAGCCAGTGATGGAAGAAGATGCGAGAGTAAGGACTCAAAATGCAATACCGTGCATAAGAAAATGGACAAAGAAGGGGAACATGACACTCTTAGGAGCATTTCAGAGACTGATCCTAGGAGTTCCTGTGTTTCAGCAAATAATGCCAGAAGTGGAACAGCTGTCCATGAAGAGAAGAGCTTCAGTAATTTTGAGCGTAAGGAAGAGGTACAGAGCAATTTAGACAAAGCCACAGAAAATGAGAAATTGGTTGTGCAGACGAGTCGAGAACAGTCTTATGATATCTCTCCATACAAAGAATCAGATGATGAAAACGACGAAGATGACGATAGTATACCAAATAATAAATTTATTCCTTCGTGGGCAAGCAAAAATTGCCTGGCTCTGGTTGCTTCTCGCCAGAGTAGAACTGACCCAGAGGTGTTCTTCCCTCCCAAAAGCTTTCCCTGTGTAGCTGAAGCCGATTTCTCCGTCATGCTGGCCTTACTGTATCAGTTTCAGAACTACAGATTGAGTTTTAGCTTTACAGTTTGGTGGATTCGGAGGAACCGCAATCTTTCTCGACACCAGAGAATGTTGTTAAAATGGCTCATGAGTTGGGAGTTGGGAATCACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
INCENP_ARK-bind

Protein Analysis

1873

Amino Acids

206.83

Weight (kDa)

5.37

Isoelectric Point (pI)

54.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
INCENP_ARK-bind PF03941 1769 - 1815 5.9e-06 Inner centromere protein, ARK binding region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3719
AasI GACNNNNNNGTC 1 cut(s) 1333
AccBSI CCGCTC 1 cut(s) 2430
AccI GTMKAC 5 cut(s) 5, 2616, 2672, 2882, 5338
AccIII TCCGGA 1 cut(s) 1867
AciI CCGC 6 cut(s) 2430, 3683, 4283, 4939, 4976, 5554
AclWI GGATC 8 cut(s) 214, 422, 435, 713, 726, 1605, 4504, 5111
AcoI YGGCCR 2 cut(s) 1237, 2131
AcsI RAATTY 7 cut(s) 604, 1172, 1958, 2066, 4057, 4379, 5351
AdeI CACNNNGTG 1 cut(s) 215
AfiI CCNNNNNNNGG 7 cut(s) 230, 800, 1166, 2174, 2452, 3578, 5389
AflIII ACRYGT 4 cut(s) 162, 1614, 1987, 3655
AhlI ACTAGT 1 cut(s) 3677
AjiI CACGTC 2 cut(s) 163, 1617
AjnI CCWGG 7 cut(s) 898, 2640, 2788, 4420, 4660, 4689, 5382
AjuI GAANNNNNNNTTGG 2 cut(s) 2986, 3018
AleI CACNNNNGTG 1 cut(s) 843
AloI GAACNNNNNNTCC 2 cut(s) 3450, 3482
Alw21I GWGCWC 4 cut(s) 305, 1984, 3652, 4169
Alw26I GTCTC 7 cut(s) 447, 475, 738, 766, 915, 1416, 5105
Alw44I GTGCAC 1 cut(s) 301
AlwI GGATC 8 cut(s) 214, 422, 435, 713, 726, 1605, 4504, 5111
AlwNI CAGNNNCTG 2 cut(s) 4328, 5114
Ama87I CYCGRG 1 cut(s) 3296
Aor13HI TCCGGA 1 cut(s) 1867
ApaI GGGCCC 1 cut(s) 1953
ApaLI GTGCAC 1 cut(s) 301
ApoI RAATTY 7 cut(s) 604, 1172, 1958, 2066, 4057, 4379, 5351
ArsI GACNNNNNNTTYG 2 cut(s) 3907, 3939
AseI ATTAAT 1 cut(s) 3221
Asp700I GAANNNNTTC 4 cut(s) 3102, 4132, 4511, 5183
AspA2I CCTAGG 1 cut(s) 5120
AsuC2I CCSGG 1 cut(s) 4019
AsuHPI GGTGA 6 cut(s) 1088, 1343, 1620, 2030, 3018, 5608
AsuII TTCGAA 1 cut(s) 1018
AvaI CYCGRG 1 cut(s) 3296
AvaII GGWCC 4 cut(s) 1520, 3514, 3618, 3945
AvrII CCTAGG 1 cut(s) 5120
AxyI CCTNAGG 1 cut(s) 1632
BaeGI GKGCMC 4 cut(s) 305, 371, 548, 1953
BaeI ACNNNNGTAYC 2 cut(s) 3444, 3477
BalI TGGCCA 2 cut(s) 1239, 2133
BamHI GGATCC 2 cut(s) 427, 718
BanII GRGCYC 4 cut(s) 1009, 1953, 4169, 4584
BbsI GAAGAC 3 cut(s) 2718, 3518, 4899
Bbv12I GWGCWC 4 cut(s) 305, 1984, 3652, 4169
BbvCI CCTCAGC 1 cut(s) 2861
BceAI ACGGC 1 cut(s) 198
BcgI CGANNNNNNTGC 2 cut(s) 5249, 5283
BciT130I CCWGG 7 cut(s) 900, 2642, 2790, 4422, 4662, 4691, 5384
BciVI GTATCC 3 cut(s) 665, 922, 3445
BclI TGATCA 3 cut(s) 1743, 2596, 3408
BcnI CCSGG 1 cut(s) 4019
BcoDI GTCTC 7 cut(s) 447, 475, 738, 766, 915, 1416, 5105
BcuI ACTAGT 1 cut(s) 3677
BfmI CTRYAG 7 cut(s) 267, 1561, 3899, 4335, 4650, 4814, 5509
BfuI GTATCC 3 cut(s) 665, 922, 3445
BglI GCCNNNNNGGC 1 cut(s) 4694
BglII AGATCT 6 cut(s) 30, 986, 1223, 2117, 3602, 3937
BlnI CCTAGG 1 cut(s) 5120
BmcAI AGTACT 1 cut(s) 1027
Bme1390I CCNGG 8 cut(s) 900, 2642, 2790, 4019, 4422, 4662, 4691, 5384
Bme18I GGWCC 4 cut(s) 1520, 3514, 3618, 3945
BmeT110I CYCGRG 1 cut(s) 3296
BmgBI CACGTC 2 cut(s) 163, 1617
BmiI GGNNCC 8 cut(s) 429, 720, 1951, 3516, 3619, 3779, 4581, 5552
BmrFI CCNGG 8 cut(s) 900, 2642, 2790, 4019, 4422, 4662, 4691, 5384
BmrI ACTGGG 3 cut(s) 1847, 2629, 3927
BmuI ACTGGG 3 cut(s) 1847, 2629, 3927
BoxI GACNNNNGTC 1 cut(s) 1395
BpiI GAAGAC 3 cut(s) 2718, 3518, 4899
BpmI CTGGAG 4 cut(s) 2889, 3633, 3836, 4712
Bpu10I CCTNAGC 2 cut(s) 2861, 4308
Bpu14I TTCGAA 1 cut(s) 1018
BpuEI CTTGAG 9 cut(s) 393, 1414, 1752, 1802, 3112, 3168, 3385, 3467, 3725
BpuMI CCSGG 1 cut(s) 4019
BsaAI YACGTR 2 cut(s) 653, 4973
BsaBI GATNNNNATC 1 cut(s) 224
BsaJI CCNNGG 6 cut(s) 2370, 3297, 3666, 4661, 4689, 5120
BsaWI WCCGGW 2 cut(s) 1867, 4493
Bsc4I CCNNNNNNNGG 7 cut(s) 230, 800, 1166, 2174, 2452, 3578, 5389
Bse118I RCCGGY 2 cut(s) 793, 3806
Bse21I CCTNAGG 1 cut(s) 1632
Bse3DI GCAATG 7 cut(s) 53, 114, 238, 352, 1063, 3699, 3744
Bse8I GATNNNNATC 1 cut(s) 224
BseAI TCCGGA 1 cut(s) 1867
BseBI CCWGG 7 cut(s) 900, 2642, 2790, 4422, 4662, 4691, 5384
BseDI CCNNGG 6 cut(s) 2370, 3297, 3666, 4661, 4689, 5120
BseJI GATNNNNATC 1 cut(s) 224
BseLI CCNNNNNNNGG 7 cut(s) 230, 800, 1166, 2174, 2452, 3578, 5389
BseMI GCAATG 7 cut(s) 53, 114, 238, 352, 1063, 3699, 3744
BseMII CTCAG 9 cut(s) 486, 777, 795, 1623, 2852, 2891, 3215, 3932, 4790
BseRI GAGGAG 6 cut(s) 123, 139, 827, 2468, 2878, 4850
BseSI GKGCMC 4 cut(s) 305, 371, 548, 1953
BseYI CCCAGC 7 cut(s) 560, 851, 958, 1417, 1544, 1952, 3422
BsgI GTGCAG 3 cut(s) 534, 2739, 5279
BsiHKAI GWGCWC 4 cut(s) 305, 1984, 3652, 4169
BsiHKCI CYCGRG 1 cut(s) 3296
BsiSI CCGG 6 cut(s) 120, 794, 1868, 3807, 4019, 4494
BslFI GGGAC 3 cut(s) 3631, 4408, 4685
BslI CCNNNNNNNGG 7 cut(s) 230, 800, 1166, 2174, 2452, 3578, 5389
BsmAI GTCTC 7 cut(s) 447, 475, 738, 766, 915, 1416, 5105
BsmBI CGTCTC 3 cut(s) 447, 738, 915
BsmFI GGGAC 3 cut(s) 3631, 4408, 4685
BsmI GAATGC 4 cut(s) 643, 2000, 2271, 2972
BsoBI CYCGRG 1 cut(s) 3296
Bsp119I TTCGAA 1 cut(s) 1018
Bsp120I GGGCCC 1 cut(s) 1949
Bsp1286I GDGCHC 9 cut(s) 305, 371, 548, 1009, 1953, 1984, 3652, 4169, 4584
Bsp13I TCCGGA 1 cut(s) 1867
Bsp1407I TGTACA 1 cut(s) 2914
BspACI CCGC 6 cut(s) 2430, 3683, 4283, 4939, 4976, 5554
BspEI TCCGGA 1 cut(s) 1867
BspHI TCATGA 3 cut(s) 175, 3067, 5595
BspLI GGNNCC 8 cut(s) 429, 720, 1951, 3516, 3619, 3779, 4581, 5552
BspMAI CTGCAG 3 cut(s) 4339, 4654, 4818
BspPI GGATC 8 cut(s) 214, 422, 435, 713, 726, 1605, 4504, 5111
BspQI GCTCTTC 2 cut(s) 2467, 5174
BspT104I TTCGAA 1 cut(s) 1018
BsrBI CCGCTC 1 cut(s) 2430
BsrDI GCAATG 7 cut(s) 53, 114, 238, 352, 1063, 3699, 3744
BsrFI RCCGGY 2 cut(s) 793, 3806
BsrGI TGTACA 1 cut(s) 2914
BssAI RCCGGY 2 cut(s) 793, 3806
BssECI CCNNGG 6 cut(s) 2370, 3297, 3666, 4661, 4689, 5120
BssNAI GTATAC 1 cut(s) 5339
BssT1I CCWWGG 3 cut(s) 2370, 3666, 5120
Bst1107I GTATAC 1 cut(s) 5339
Bst2UI CCWGG 7 cut(s) 900, 2642, 2790, 4422, 4662, 4691, 5384
BstAPI GCANNNNNTGC 3 cut(s) 25, 5147, 5379
BstAUI TGTACA 1 cut(s) 2914
BstBAI YACGTR 2 cut(s) 653, 4973
BstBI TTCGAA 1 cut(s) 1018
BstENI CCTNNNNNAGG 1 cut(s) 2450
BstMAI GTCTC 7 cut(s) 447, 475, 738, 766, 915, 1416, 5105
BstNI CCWGG 7 cut(s) 900, 2642, 2790, 4422, 4662, 4691, 5384
BstNSI RCATGY 4 cut(s) 1991, 3659, 3844, 3935
BstPAI GACNNNNGTC 1 cut(s) 1395
BstSCI CCNGG 8 cut(s) 898, 2640, 2788, 4017, 4420, 4660, 4689, 5382
BstSFI CTRYAG 7 cut(s) 267, 1561, 3899, 4335, 4650, 4814, 5509
BstSLI GKGCMC 4 cut(s) 305, 371, 548, 1953
BstSNI TACGTA 1 cut(s) 653
BstV2I GAAGAC 3 cut(s) 2718, 3518, 4899
BstX2I RGATCY 8 cut(s) 30, 427, 718, 986, 1223, 2117, 3602, 3937
BstXI CCANNNNNNTGG 1 cut(s) 2416
BstYI RGATCY 8 cut(s) 30, 427, 718, 986, 1223, 2117, 3602, 3937
BstZ17I GTATAC 1 cut(s) 5339
Bsu36I CCTNAGG 1 cut(s) 1632
BsuI GTATCC 3 cut(s) 665, 922, 3445
BtrI CACGTC 2 cut(s) 163, 1617
BtsI GCAGTG 2 cut(s) 2727, 3789
CaiI CAGNNNCTG 2 cut(s) 4328, 5114
CciI TCATGA 3 cut(s) 175, 3067, 5595
Cfr10I RCCGGY 2 cut(s) 793, 3806
CseI GACGC 3 cut(s) 2466, 4396, 4703
CspCI CAANNNNNGTGG 2 cut(s) 1344, 1379
DraI TTTAAA 2 cut(s) 2151, 2232
DraIII CACNNNGTG 1 cut(s) 215
DrdI GACNNNNNNGTC 1 cut(s) 1333
DseDI GACNNNNNNGTC 1 cut(s) 1333
EaeI YGGCCR 2 cut(s) 1237, 2131
EciI GGCGGA 1 cut(s) 4954
Ecl136II GAGCTC 1 cut(s) 4167
Eco105I TACGTA 1 cut(s) 653
Eco130I CCWWGG 3 cut(s) 2370, 3666, 5120
Eco147I AGGCCT 1 cut(s) 77
Eco24I GRGCYC 4 cut(s) 1009, 1953, 4169, 4584
Eco32I GATATC 2 cut(s) 1098, 5287
Eco47I GGWCC 4 cut(s) 1520, 3514, 3618, 3945
Eco53kI GAGCTC 1 cut(s) 4167
Eco81I CCTNAGG 1 cut(s) 1632
Eco88I CYCGRG 1 cut(s) 3296
EcoICRI GAGCTC 1 cut(s) 4167
EcoNI CCTNNNNNAGG 1 cut(s) 2450
EcoO109I RGGNCCY 5 cut(s) 1520, 1949, 3514, 4559, 4687
EcoRI GAATTC 2 cut(s) 604, 4379
EcoRII CCWGG 7 cut(s) 898, 2640, 2788, 4420, 4660, 4689, 5382
EcoRV GATATC 2 cut(s) 1098, 5287
EcoT14I CCWWGG 3 cut(s) 2370, 3666, 5120
EcoT38I GRGCYC 4 cut(s) 1009, 1953, 4169, 4584
ErhI CCWWGG 3 cut(s) 2370, 3666, 5120
Esp3I CGTCTC 3 cut(s) 447, 738, 915
FalI AAGNNNNNCTT 2 cut(s) 3090, 3122
FaqI GGGAC 3 cut(s) 3631, 4408, 4685
FbaI TGATCA 3 cut(s) 1743, 2596, 3408
FblI GTMKAC 5 cut(s) 5, 2616, 2672, 2882, 5338
FriOI GRGCYC 4 cut(s) 1009, 1953, 4169, 4584
GsaI CCCAGC 7 cut(s) 564, 855, 962, 1421, 1548, 1956, 3426
GsuI CTGGAG 4 cut(s) 2889, 3633, 3836, 4712
HapII CCGG 6 cut(s) 120, 794, 1868, 3807, 4019, 4494
HgaI GACGC 3 cut(s) 2466, 4396, 4703
HincII GTYRAC 2 cut(s) 6, 2883
HindII GTYRAC 2 cut(s) 6, 2883
HindIII AAGCTT 2 cut(s) 4310, 5443
HpaII CCGG 6 cut(s) 120, 794, 1868, 3807, 4019, 4494
HphI GGTGA 6 cut(s) 1088, 1343, 1620, 2030, 3018, 5608
Hpy99I CGWCG 3 cut(s) 10, 164, 5324
HpyCH4IV ACGT 9 cut(s) 162, 440, 652, 731, 768, 908, 1616, 4741, 4972
HpySE526I ACGT 9 cut(s) 162, 440, 652, 731, 768, 908, 1616, 4741, 4972
Kpn2I TCCGGA 1 cut(s) 1867
Ksp22I TGATCA 3 cut(s) 1743, 2596, 3408
LguI GCTCTTC 2 cut(s) 2467, 5174
MaeII ACGT 9 cut(s) 162, 440, 652, 731, 768, 908, 1616, 4741, 4972
MaeIII GTNAC 9 cut(s) 436, 694, 727, 2360, 2478, 2867, 4369, 4589, 4968
MbiI CCGCTC 1 cut(s) 2430
MfeI CAATTG 2 cut(s) 1554, 2392
MflI RGATCY 8 cut(s) 30, 427, 718, 986, 1223, 2117, 3602, 3937
MhlI GDGCHC 9 cut(s) 305, 371, 548, 1009, 1953, 1984, 3652, 4169, 4584
MlsI TGGCCA 2 cut(s) 1239, 2133
MluNI TGGCCA 2 cut(s) 1239, 2133
MlyI GAGTC 9 cut(s) 477, 768, 773, 1576, 2231, 3244, 4390, 5033, 5275
MmeI TCCRAC 4 cut(s) 1829, 3494, 3938, 4788
Mox20I TGGCCA 2 cut(s) 1239, 2133
MroI TCCGGA 1 cut(s) 1867
MroXI GAANNNNTTC 4 cut(s) 3102, 4132, 4511, 5183
MscI TGGCCA 2 cut(s) 1239, 2133
MslI CAYNNNNRTG 6 cut(s) 243, 843, 1590, 2487, 3258, 4679
Msp20I TGGCCA 2 cut(s) 1239, 2133
MspA1I CMGCKG 2 cut(s) 4649, 5165
MspI CCGG 6 cut(s) 120, 794, 1868, 3807, 4019, 4494
MspR9I CCNGG 8 cut(s) 900, 2642, 2790, 4019, 4422, 4662, 4691, 5384
MunI CAATTG 2 cut(s) 1554, 2392
Mva1269I GAATGC 4 cut(s) 643, 2000, 2271, 2972
MvaI CCWGG 7 cut(s) 900, 2642, 2790, 4422, 4662, 4691, 5384
NciI CCSGG 1 cut(s) 4019
NlaIV GGNNCC 8 cut(s) 429, 720, 1951, 3516, 3619, 3779, 4581, 5552
NmuCI GTSAC 2 cut(s) 694, 2478
NspI RCATGY 4 cut(s) 1991, 3659, 3844, 3935
NspV TTCGAA 1 cut(s) 1018
OliI CACNNNNGTG 1 cut(s) 843
PaeI GCATGC 1 cut(s) 3844
PagI TCATGA 3 cut(s) 175, 3067, 5595
PceI AGGCCT 1 cut(s) 77
PciI ACATGT 2 cut(s) 1987, 3655
PciSI GCTCTTC 2 cut(s) 2467, 5174
PcsI WCGNNNNNNNCGW 2 cut(s) 211, 5328
PctI GAATGC 4 cut(s) 643, 2000, 2271, 2972
PdmI GAANNNNTTC 4 cut(s) 3102, 4132, 4511, 5183
PleI GAGTC 9 cut(s) 476, 767, 773, 1575, 2230, 3243, 4390, 5033, 5274
PpsI GAGTC 9 cut(s) 476, 767, 773, 1575, 2230, 3243, 4390, 5033, 5274
Ppu21I YACGTR 2 cut(s) 653, 4973
PpuMI RGGWCCY 2 cut(s) 1520, 3514
PscI ACATGT 2 cut(s) 1987, 3655
PshAI GACNNNNGTC 1 cut(s) 1395
PshBI ATTAAT 1 cut(s) 3221
PsiI TTATAA 1 cut(s) 3719
Psp124BI GAGCTC 1 cut(s) 4169
Psp5II RGGWCCY 2 cut(s) 1520, 3514
Psp6I CCWGG 7 cut(s) 898, 2640, 2788, 4420, 4660, 4689, 5382
PspFI CCCAGC 7 cut(s) 560, 851, 958, 1417, 1544, 1952, 3422
PspGI CCWGG 7 cut(s) 898, 2640, 2788, 4420, 4660, 4689, 5382
PspN4I GGNNCC 8 cut(s) 429, 720, 1951, 3516, 3619, 3779, 4581, 5552
PspOMI GGGCCC 1 cut(s) 1949
PspPPI RGGWCCY 2 cut(s) 1520, 3514
PstI CTGCAG 3 cut(s) 4339, 4654, 4818
PstNI CAGNNNCTG 2 cut(s) 4328, 5114
PsuI RGATCY 8 cut(s) 30, 427, 718, 986, 1223, 2117, 3602, 3937
PvuII CAGCTG 2 cut(s) 4649, 5165
RseI CAYNNNNRTG 6 cut(s) 243, 843, 1590, 2487, 3258, 4679
SacI GAGCTC 1 cut(s) 4169
SalI GTCGAC 2 cut(s) 4, 2881
SapI GCTCTTC 2 cut(s) 2467, 5174
ScaI AGTACT 1 cut(s) 1027
SchI GAGTC 9 cut(s) 477, 768, 773, 1576, 2231, 3244, 4390, 5033, 5275
ScrFI CCNGG 8 cut(s) 900, 2642, 2790, 4019, 4422, 4662, 4691, 5384
SduI GDGCHC 9 cut(s) 305, 371, 548, 1009, 1953, 1984, 3652, 4169, 4584
SfcI CTRYAG 7 cut(s) 267, 1561, 3899, 4335, 4650, 4814, 5509
SfuI TTCGAA 1 cut(s) 1018
SinI GGWCC 4 cut(s) 1520, 3514, 3618, 3945
SmiMI CAYNNNNRTG 6 cut(s) 243, 843, 1590, 2487, 3258, 4679
SmlI CTYRAG 9 cut(s) 408, 1429, 1767, 1781, 3091, 3183, 3400, 3446, 3704
SmoI CTYRAG 9 cut(s) 408, 1429, 1767, 1781, 3091, 3183, 3400, 3446, 3704
SnaBI TACGTA 1 cut(s) 653
SpeI ACTAGT 1 cut(s) 3677
SphI GCATGC 1 cut(s) 3844
SseBI AGGCCT 1 cut(s) 77
SsiI CCGC 6 cut(s) 2430, 3683, 4283, 4939, 4976, 5554
SspI AATATT 2 cut(s) 4597, 4609
SstI GAGCTC 1 cut(s) 4169
StuI AGGCCT 1 cut(s) 77
StyD4I CCNGG 8 cut(s) 898, 2640, 2788, 4017, 4420, 4660, 4689, 5382
StyI CCWWGG 3 cut(s) 2370, 3666, 5120
TaiI ACGT 9 cut(s) 165, 443, 655, 734, 771, 911, 1619, 4744, 4975
TatI WGTACW 3 cut(s) 1025, 2914, 3700
TseFI GTSAC 2 cut(s) 694, 2478
Tsp45I GTSAC 2 cut(s) 694, 2478
TspGWI ACGGA 4 cut(s) 320, 2543, 2711, 5464
VneI GTGCAC 1 cut(s) 301
VpaK11BI GGWCC 4 cut(s) 1520, 3514, 3618, 3945
VspI ATTAAT 1 cut(s) 3221
XagI CCTNNNNNAGG 1 cut(s) 2450
XapI RAATTY 7 cut(s) 604, 1172, 1958, 2066, 4057, 4379, 5351
XbaI TCTAGA 5 cut(s) 1226, 2120, 2468, 3433, 4360
XceI RCATGY 4 cut(s) 1991, 3659, 3844, 3935
XcmI CCANNNNNNNNNTGG 1 cut(s) 5012
XmaJI CCTAGG 1 cut(s) 5120
XmiI GTMKAC 5 cut(s) 5, 2616, 2672, 2882, 5338
XmnI GAANNNNTTC 4 cut(s) 3102, 4132, 4511, 5183
ZrmI AGTACT 1 cut(s) 1027
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.