AT5G67610

NEMP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0001753
Physical Location & Seq
Reverse (-)
26960904 .. 26963736
2833 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G67610.1

Sequence Viewer

Length: 1461 bp
ATGGGTGATTATCGTCTTCTACGAAACTCCGGCGTCGTTATCGGAATAATCTTGCTACATCTCTTTACTTTCGCCTCTCTTGTTTCATCCGATGAACTTCAGTTTGTTGTGGGAGAAAGCGGAGAGCTTCAAGTGACTCCTAGTTTAGAGGTAAAAGGTTCTCCTGGATTGAAGCCAGATAGAACCTCTCTTTGTGAGAGGATTCATATCCATGGACTTGGAAGGTTTAAACATCTAGACAAGTATGCACATTCGCTCAAGTTGATAGTTAACGCGTCAATCTCTGGGAAAACAAATAACATTGACGTTTGCTTTCACCGGAATTTGTCGCGTGGAATTGGAATGTGCCCTCATAGTCGATGGGAGAAAGCCTCTAAAGGTTCATGGGTTCAGACAATGTCACCCTTCGACCATAAAATCCTTGATGTTAGAGTACCTAGCTCTAACAAGGTCTCATTGGAAGTGTCTGCTGTAGAAGAATTATTTATGCATCGCATAGTATTCTTACTCCTTGGTGCTGTATTACTGGCTTCGGCTTCTACACTGAGCCAATCTTTAGCGTTTTACTACAGTAGTGCAATGGCTGTTGGTATTATCCTTGTCGTGCTGCTTGTTCTCTTTCAGGGAATGAAGCTTCTACCTACTGGACGGAGTTCGTTTGCATTGTTCATATACTCAACCCTGCTTGGTTTGGGTGGTTTTCTTCTTCGCTACTTACCCGGATTGTTTGAAAGTCTGCTAACAGAGATGGGAATCGACGAAGAAATGTACACACCTGCGGCGATTTTTGTGGGGGCGTTTCTGTCTTTGGGTGGAGCATTTTTTGGATTTTGGACTGTGAGGAAACTTATTCTGACGGAAGATGGCTCCATTGATGTTAGCACATCACTATTTGTTTCTTGGTCCATCCGAATTATGGCTGCTGTTTTGATCCTTCAGAGCTCTGTAGATCCTTTACTTGCTGGAGGAGCCCTGATATCTGTAATTCTTATGTCATCGACTTTAAAGAAGATCACCAGATTGAAGTTCCTTCTACGCTTATTCGAGATTCCATTGAATTTACTGCTAGGAATCTGGGAGGCAATTCGTGATACTGATATACCATCCGTCCCGGGATATCTCCATGACTTTATGCAAAAGAGTCCTGATGCTTCTGGATTTCGCAACCGTGTAACTTCTGCTTCTCCATCAGGAGGAATCAACAACGGGATGCGAGAATCTCCACCGTCTGAATCAGATACATTTCCTTCCTCTTTCCATAAAACTCCTGAAAGGAGCCAACTAACAAAAGAAGAATGGAAAAAGCTCACTAAGGATAGCACGACAAAGGCTGTTCAAGAATTGGTTTCTTCACCCGATTTTGGCAAATGGGCAGCAGTTAATGCGGATAGGATCAATGTAACACCAAGAAAAGGATCTTCTAGCAAAAACCAGCCACGGAAATGGATGCGTTGGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NEMP

Protein Analysis

486

Amino Acids

53.64

Weight (kDa)

9.4

Isoelectric Point (pI)

43.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NEMP PF10225 155 - 334 1.3e-37 NEMP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 784
Acc36I ACCTGC 1 cut(s) 784
AccII CGCG 2 cut(s) 275, 331
AciI CCGC 3 cut(s) 120, 779, 1385
AclWI GGATC 4 cut(s) 925, 944, 1400, 1423
AcsI RAATTY 2 cut(s) 322, 1057
AcuI CTGAAG 2 cut(s) 83, 920
AcyI GRCGYC 1 cut(s) 33
AfaI GTAC 2 cut(s) 435, 770
AfiI CCNNNNNNNGG 4 cut(s) 916, 1193, 1361, 1412
AflIII ACRYGT 1 cut(s) 273
AgsI TTSAA 6 cut(s) 131, 172, 731, 1024, 1057, 1337
AjnI CCWGG 1 cut(s) 163
AluBI AGCT 5 cut(s) 127, 441, 634, 942, 1306
AluI AGCT 5 cut(s) 127, 441, 634, 942, 1306
Alw21I GWGCWC 1 cut(s) 944
Alw26I GTCTC 1 cut(s) 457
AlwI GGATC 4 cut(s) 925, 944, 1400, 1423
Ama87I CYCGRG 1 cut(s) 1111
ApeKI GCWGC 3 cut(s) 607, 920, 1373
ApoI RAATTY 2 cut(s) 322, 1057
AspS9I GGNCC 1 cut(s) 903
AsuC2I CCSGG 3 cut(s) 720, 1112, 1113
AsuHPI GGTGA 5 cut(s) 17, 308, 393, 1006, 1344
AvaI CYCGRG 1 cut(s) 1111
AvaII GGWCC 1 cut(s) 903
BaeGI GKGCMC 1 cut(s) 350
BanII GRGCYC 2 cut(s) 944, 973
BbsI GAAGAC 1 cut(s) 8
Bbv12I GWGCWC 1 cut(s) 944
BbvI GCAGC 3 cut(s) 594, 907, 1385
BccI CCATC 6 cut(s) 354, 742, 857, 914, 1111, 1195
BciT130I CCWGG 1 cut(s) 165
BcnI CCSGG 3 cut(s) 720, 1112, 1113
BcoDI GTCTC 1 cut(s) 457
BfaI CTAG 5 cut(s) 141, 236, 438, 1067, 1422
BfmI CTRYAG 3 cut(s) 471, 568, 945
BfuAI ACCTGC 1 cut(s) 784
BisI GCNGC 4 cut(s) 608, 780, 921, 1374
BlsI GCNGC 4 cut(s) 609, 781, 922, 1375
Bme1390I CCNGG 4 cut(s) 165, 720, 1112, 1113
Bme18I GGWCC 1 cut(s) 903
BmeT110I CYCGRG 1 cut(s) 1111
BmgT120I GGNCC 1 cut(s) 903
BmiI GGNNCC 3 cut(s) 868, 970, 1277
BmrFI CCNGG 4 cut(s) 165, 720, 1112, 1113
BmsI GCATC 4 cut(s) 499, 1138, 1200, 1437
BpiI GAAGAC 1 cut(s) 8
BplI GAGNNNNNCTC 2 cut(s) 356, 388
BpmI CTGGAG 1 cut(s) 984
BpuEI CTTGAG 1 cut(s) 242
BpuMI CCSGG 3 cut(s) 720, 1112, 1113
BsaBI GATNNNNATC 2 cut(s) 206, 752
BsaHI GRCGYC 1 cut(s) 33
BsaI GGTCTC 1 cut(s) 457
BsaJI CCNNGG 4 cut(s) 211, 511, 1111, 1436
BsaWI WCCGGW 1 cut(s) 318
Bsc4I CCNNNNNNNGG 4 cut(s) 916, 1193, 1361, 1412
Bse1I ACTGG 2 cut(s) 531, 649
Bse3DI GCAATG 1 cut(s) 585
Bse8I GATNNNNATC 2 cut(s) 206, 752
BseBI CCWGG 1 cut(s) 165
BseDI CCNNGG 4 cut(s) 211, 511, 1111, 1436
BseGI GGATG 5 cut(s) 86, 906, 1103, 1215, 1452
BseJI GATNNNNATC 2 cut(s) 206, 752
BseLI CCNNNNNNNGG 4 cut(s) 916, 1193, 1361, 1412
BseMI GCAATG 1 cut(s) 585
BseMII CTCAG 1 cut(s) 536
BseNI ACTGG 2 cut(s) 531, 649
BseRI GAGGAG 1 cut(s) 981
BseSI GKGCMC 1 cut(s) 350
BseXI GCAGC 3 cut(s) 594, 907, 1385
Bsh1236I CGCG 2 cut(s) 275, 331
BsiHKAI GWGCWC 1 cut(s) 944
BsiHKCI CYCGRG 1 cut(s) 1111
BsiSI CCGG 4 cut(s) 30, 319, 720, 1112
BslFI GGGAC 1 cut(s) 1094
BslI CCNNNNNNNGG 4 cut(s) 916, 1193, 1361, 1412
BsmAI GTCTC 1 cut(s) 457
BsmFI GGGAC 1 cut(s) 1094
Bso31I GGTCTC 1 cut(s) 457
BsoBI CYCGRG 1 cut(s) 1111
Bsp1286I GDGCHC 3 cut(s) 350, 944, 973
Bsp1407I TGTACA 1 cut(s) 768
Bsp143I GATC 5 cut(s) 930, 949, 1011, 1392, 1415
Bsp19I CCATGG 1 cut(s) 211
BspACI CCGC 3 cut(s) 120, 779, 1385
BspCNI CTCAG 1 cut(s) 537
BspFNI CGCG 2 cut(s) 275, 331
BspLI GGNNCC 3 cut(s) 868, 970, 1277
BspMI ACCTGC 1 cut(s) 784
BspPI GGATC 4 cut(s) 925, 944, 1400, 1423
BspTNI GGTCTC 1 cut(s) 457
BsrDI GCAATG 1 cut(s) 585
BsrGI TGTACA 1 cut(s) 768
BsrI ACTGG 2 cut(s) 531, 649
BssECI CCNNGG 4 cut(s) 211, 511, 1111, 1436
BssMI GATC 5 cut(s) 930, 949, 1011, 1392, 1415
BssNI GRCGYC 1 cut(s) 33
BssT1I CCWWGG 2 cut(s) 211, 511
Bst2UI CCWGG 1 cut(s) 165
Bst4CI ACNGT 4 cut(s) 572, 838, 1169, 1227
BstACI GRCGYC 1 cut(s) 33
BstAPI GCANNNNNTGC 1 cut(s) 1382
BstAUI TGTACA 1 cut(s) 768
BstDEI CTNAG 2 cut(s) 545, 1311
BstDSI CCRYGG 2 cut(s) 211, 1436
BstF5I GGATG 5 cut(s) 86, 906, 1103, 1215, 1452
BstFNI CGCG 2 cut(s) 275, 331
BstKTI GATC 5 cut(s) 933, 952, 1014, 1395, 1418
BstMAI GTCTC 1 cut(s) 457
BstMBI GATC 5 cut(s) 930, 949, 1011, 1392, 1415
BstMWI GCNNNNNNNGC 2 cut(s) 968, 1382
BstNI CCWGG 1 cut(s) 165
BstSCI CCNGG 4 cut(s) 163, 718, 1110, 1111
BstSFI CTRYAG 3 cut(s) 471, 568, 945
BstSLI GKGCMC 1 cut(s) 350
BstUI CGCG 2 cut(s) 275, 331
BstV1I GCAGC 3 cut(s) 594, 907, 1385
BstV2I GAAGAC 1 cut(s) 8
BstX2I RGATCY 2 cut(s) 949, 1415
BstXI CCANNNNNNTGG 2 cut(s) 218, 1443
BstYI RGATCY 2 cut(s) 949, 1415
BtgI CCRYGG 2 cut(s) 211, 1436
BtgZI GCGATG 1 cut(s) 476
BtsCI GGATG 5 cut(s) 86, 906, 1103, 1215, 1452
BtsIMutI CAGTG 1 cut(s) 542
BveI ACCTGC 1 cut(s) 784
Cfr13I GGNCC 1 cut(s) 903
Cfr9I CCCGGG 1 cut(s) 1111
CseI GACGC 2 cut(s) 22, 264
Csp6I GTAC 2 cut(s) 434, 769
CviAII CATG 3 cut(s) 212, 384, 1124
CviQI GTAC 2 cut(s) 434, 769
DdeI CTNAG 2 cut(s) 545, 1311
DpnI GATC 5 cut(s) 932, 951, 1013, 1394, 1417
DpnII GATC 5 cut(s) 930, 949, 1011, 1392, 1415
DraI TTTAAA 2 cut(s) 229, 1005
Ecl136II GAGCTC 1 cut(s) 942
Eco130I CCWWGG 2 cut(s) 211, 511
Eco24I GRGCYC 2 cut(s) 944, 973
Eco31I GGTCTC 1 cut(s) 457
Eco32I GATATC 2 cut(s) 978, 1118
Eco47I GGWCC 1 cut(s) 903
Eco53kI GAGCTC 1 cut(s) 942
Eco57I CTGAAG 2 cut(s) 83, 920
Eco88I CYCGRG 1 cut(s) 1111
EcoICRI GAGCTC 1 cut(s) 942
EcoRII CCWGG 1 cut(s) 163
EcoRV GATATC 2 cut(s) 978, 1118
EcoT14I CCWWGG 2 cut(s) 211, 511
EcoT22I ATGCAT 1 cut(s) 492
EcoT38I GRGCYC 2 cut(s) 944, 973
ErhI CCWWGG 2 cut(s) 211, 511
FaeI CATG 3 cut(s) 215, 387, 1127
FaqI GGGAC 1 cut(s) 1094
FatI CATG 3 cut(s) 211, 383, 1123
Fnu4HI GCNGC 4 cut(s) 608, 780, 921, 1374
FokI GGATG 4 cut(s) 73, 893, 1090, 1222
FriOI GRGCYC 2 cut(s) 944, 973
Fsp4HI GCNGC 4 cut(s) 608, 780, 921, 1374
FspBI CTAG 5 cut(s) 141, 236, 438, 1067, 1422
GluI GCNGC 4 cut(s) 608, 780, 921, 1374
GsuI CTGGAG 1 cut(s) 984
HapII CCGG 4 cut(s) 30, 319, 720, 1112
HgaI GACGC 2 cut(s) 22, 264
Hin1I GRCGYC 1 cut(s) 33
Hin1II CATG 3 cut(s) 215, 387, 1127
HincII GTYRAC 1 cut(s) 271
HindII GTYRAC 1 cut(s) 271
HindIII AAGCTT 1 cut(s) 632
HinfI GANTC 9 cut(s) 136, 202, 753, 1048, 1071, 1141, 1197, 1217, 1232
HpaI GTTAAC 1 cut(s) 271
HpaII CCGG 4 cut(s) 30, 319, 720, 1112
HphI GGTGA 5 cut(s) 17, 308, 393, 1006, 1344
Hpy166II GTNNAC 2 cut(s) 271, 771
Hpy188I TCNGA 9 cut(s) 44, 91, 393, 855, 911, 939, 1231, 1237, 1460
Hpy188III TCNNGA 8 cut(s) 236, 1045, 1088, 1145, 1155, 1191, 1268, 1337
Hpy8I GTNNAC 2 cut(s) 271, 771
Hpy99I CGWCG 2 cut(s) 38, 761
HpyAV CCTTC 5 cut(s) 216, 415, 944, 1040, 1257
HpyCH4III ACNGT 4 cut(s) 572, 838, 1169, 1227
HpyCH4IV ACGT 1 cut(s) 306
HpyCH4V TGCA 5 cut(s) 248, 490, 578, 662, 1135
HpyF10VI GCNNNNNNNGC 2 cut(s) 968, 1382
HpyF3I CTNAG 2 cut(s) 545, 1311
HpySE526I ACGT 1 cut(s) 306
Hsp92I GRCGYC 1 cut(s) 33
Hsp92II CATG 3 cut(s) 215, 387, 1127
KspAI GTTAAC 1 cut(s) 271
Kzo9I GATC 5 cut(s) 930, 949, 1011, 1392, 1415
LmnI GCTCC 4 cut(s) 815, 872, 968, 1275
Lsp1109I GCAGC 3 cut(s) 594, 907, 1385
LweI GCATC 4 cut(s) 499, 1138, 1200, 1437
MaeI CTAG 5 cut(s) 141, 236, 438, 1067, 1422
MaeII ACGT 1 cut(s) 306
MaeIII GTNAC 4 cut(s) 133, 399, 1171, 1399
MalI GATC 5 cut(s) 932, 951, 1013, 1394, 1417
MboI GATC 5 cut(s) 930, 949, 1011, 1392, 1415
MflI RGATCY 2 cut(s) 949, 1415
MhlI GDGCHC 3 cut(s) 350, 944, 973
MluCI AATT 8 cut(s) 322, 336, 479, 912, 984, 1057, 1083, 1340
MluI ACGCGT 1 cut(s) 273
MlyI GAGTC 2 cut(s) 130, 1150
Mph1103I ATGCAT 1 cut(s) 492
MseI TTAA 4 cut(s) 228, 270, 1004, 1380
MslI CAYNNNNRTG 2 cut(s) 210, 1441
MspI CCGG 4 cut(s) 30, 319, 720, 1112
MspR9I CCNGG 4 cut(s) 165, 720, 1112, 1113
MssI GTTTAAAC 1 cut(s) 229
MvaI CCWGG 1 cut(s) 165
MvnI CGCG 2 cut(s) 275, 331
MwoI GCNNNNNNNGC 2 cut(s) 968, 1382
NciI CCSGG 3 cut(s) 720, 1112, 1113
NcoI CCATGG 1 cut(s) 211
NdeII GATC 5 cut(s) 930, 949, 1011, 1392, 1415
NlaIII CATG 3 cut(s) 215, 387, 1127
NlaIV GGNNCC 3 cut(s) 868, 970, 1277
NmuCI GTSAC 2 cut(s) 133, 399
NsiI ATGCAT 1 cut(s) 492
PaqCI CACCTGC 1 cut(s) 784
PcsI WCGNNNNNNNCGW 1 cut(s) 19
PfeI GAWTC 7 cut(s) 202, 753, 1048, 1071, 1197, 1217, 1232
PflFI GACNNNGTC 1 cut(s) 397
PfoI TCCNGGA 1 cut(s) 163
PkrI GCNGC 4 cut(s) 609, 781, 922, 1375
PleI GAGTC 2 cut(s) 130, 1149
PmeI GTTTAAAC 1 cut(s) 229
PpsI GAGTC 2 cut(s) 130, 1149
Psp124BI GAGCTC 1 cut(s) 944
Psp6I CCWGG 1 cut(s) 163
PspGI CCWGG 1 cut(s) 163
PspN4I GGNNCC 3 cut(s) 868, 970, 1277
PspPI GGNCC 1 cut(s) 903
PsuI RGATCY 2 cut(s) 949, 1415
PsyI GACNNNGTC 1 cut(s) 397
RsaI GTAC 2 cut(s) 435, 770
RsaNI GTAC 2 cut(s) 434, 769
RseI CAYNNNNRTG 2 cut(s) 210, 1441
SacI GAGCTC 1 cut(s) 944
SaqAI TTAA 4 cut(s) 228, 270, 1004, 1380
SatI GCNGC 4 cut(s) 608, 780, 921, 1374
Sau3AI GATC 5 cut(s) 930, 949, 1011, 1392, 1415
Sau96I GGNCC 1 cut(s) 903
SchI GAGTC 2 cut(s) 130, 1150
ScrFI CCNGG 4 cut(s) 165, 720, 1112, 1113
SduI GDGCHC 3 cut(s) 350, 944, 973
SfaNI GCATC 4 cut(s) 499, 1138, 1200, 1437
SfcI CTRYAG 3 cut(s) 471, 568, 945
SinI GGWCC 1 cut(s) 903
SmaI CCCGGG 1 cut(s) 1113
SmiMI CAYNNNNRTG 2 cut(s) 210, 1441
SmlI CTYRAG 1 cut(s) 257
SmoI CTYRAG 1 cut(s) 257
Sse9I AATT 8 cut(s) 322, 336, 479, 912, 984, 1057, 1083, 1340
SsiI CCGC 3 cut(s) 120, 779, 1385
SspMI CTAG 5 cut(s) 141, 236, 438, 1067, 1422
SstI GAGCTC 1 cut(s) 944
StyD4I CCNGG 4 cut(s) 163, 718, 1110, 1111
StyI CCWWGG 2 cut(s) 211, 511
TaaI ACNGT 4 cut(s) 572, 838, 1169, 1227
TaiI ACGT 1 cut(s) 309
TaqI TCGA 5 cut(s) 358, 408, 756, 998, 1044
TasI AATT 8 cut(s) 322, 336, 479, 912, 984, 1057, 1083, 1340
TatI WGTACW 1 cut(s) 768
TauI GCSGC 1 cut(s) 782
TfiI GAWTC 7 cut(s) 202, 753, 1048, 1071, 1197, 1217, 1232
Tru1I TTAA 4 cut(s) 228, 270, 1004, 1380
Tru9I TTAA 4 cut(s) 228, 270, 1004, 1380
TscAI CASTG 1 cut(s) 549
TseFI GTSAC 2 cut(s) 133, 399
TseI GCWGC 3 cut(s) 607, 920, 1373
Tsp45I GTSAC 2 cut(s) 133, 399
TspDTI ATGAA 6 cut(s) 75, 108, 194, 372, 644, 658
TspGWI ACGGA 4 cut(s) 664, 872, 1096, 1453
TspMI CCCGGG 1 cut(s) 1111
TspRI CASTG 1 cut(s) 549
Tth111I GACNNNGTC 1 cut(s) 397
VpaK11BI GGWCC 1 cut(s) 903
XapI RAATTY 2 cut(s) 322, 1057
XbaI TCTAGA 1 cut(s) 235
XcmI CCANNNNNNNNNTGG 1 cut(s) 913
XmaI CCCGGG 1 cut(s) 1111
XspI CTAG 5 cut(s) 141, 236, 438, 1067, 1422
Zsp2I ATGCAT 1 cut(s) 492
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.