Rorug02G0152100

NEMP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001753
Physical Location & Seq
Reverse (-)
13439126 .. 13440669
1544 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0152100.1

Sequence Viewer

Length: 1485 bp
ATGGAGATCACAAACCAACATGCTGCACTACTCTGCAGCCCAGGTATGGGCCACATCATCCCTGTCCTTGAGCTCGGGAAACGACTCGTCACCCACCAAAATTTCACTGCCACAATCTTCGTCGTCCCATTGCACACTTCCAAGGTCGAATCTGAACTCCTCGACGCATCTGCCTTCCCACGTAGTCTGGACATCATCGAGCTCCCATCACCTGATGTTTCTGGCCTTGTCGACCCCGATGCTGTCGTCGTCACACGCCTCGCAGAAATGATGCGACAAGTACGCCCTGCGTTTCGTGCCGCCTTACACGACATGATCAAGTTTCGTCATCGTCGCCCCACCATGCTCATCGTGGACCTATTCGGCACCGAATCTTTGCCCATTGCCGAGGAGTTTGGGATTCCAAAGTATGTGTACATTGCTTCCAATGCATGGTTCCTTTCTGTAATGATTTATTCTCCAACACTAGATGACCAAGTCAAGGGTAAATTTGTTGACCTCGAAGATCCACTAGAAATTCCTGGATGTAGACAATTATTACCAGAACTTGATATCGTTGACGCTTTGCTGGACCGGAGTAATCAGCAGTACGACGAGCACATGCTGATGGGAAGAGGAATCCCAAAGGGTGATGGAATTTTGATAAACATATGGGAGGAACTAGAGCCCAAGTCCCTAGCAGCACTAAGAGATGAGAAATTCTTGGGCCGTTTTGCGAATGTGCCTGTCTTTCCAATTGGGCCGTTAATGAGGCCCAATGTATCTTCCGGCTTGAGGGGTGATGTGTTTGATTGGCTAGATGAGCAGCCCAATGAATCTGTGATTTACGTGTCCTTTGGAAGCGGTGGGGCCTTGTCGTATGAACAAATGGTCGAGCTAGCTTGGGGTTTGGAGATGAGTCAACAGAGGTTCGTTTGGGTCATTCGGCCGCCTATCATATCATCTGCCGACGAAATATTTTTCACGGCAGGGAATCACAATGGTGAGAACAACAATCTAACTAAATACTTGCCCAAGGGGTTTTTGGGCCGAACCAAGGACATTGGGTTTGTCATCCCTTTGTGGGCTCCACAAGTGGATATCTTGGCCCATCCATCGGTCGGAGGGTTTTTGACACATTGCGGGTGGAACTCAACCCTTGAAAGCATCACCAATGGAGTACCAATGATCGCTTGGCCGCTCTACGCTGAGCAAAGGTTGAATGCTACGTTGTTGACAGAGGAGCTAGGGGTGGCGGTTCGGTCAAAGATACCCCCATGGAAGAATGTGGTGGAGAGAGAAGAGATAGAGGAAATGGTGAGGAAAATTATGGAGGACGAAGAAGGTTTTGCATTGAGAGATAGAGCCAAGAATCTTAAATTCAGTGGGGCAAAAGCTTTGGAACAAGGTGGTTCGTCTTATGATGCTCTTTCTCAGTTCACTAAACATGCTGACATGTTAGTGCAATTTGCAAATGGTGTTGATTCTTCTTCCCAAGTTCCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NEMP

Protein Analysis

494

Amino Acids

55.11

Weight (kDa)

5.0

Isoelectric Point (pI)

40.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UDPGT PF00201 270 - 414 2.5e-21 UDP-glucoronosyl and UDP-glucosyl transferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 365
AccB7I CCANNNNNTGG 1 cut(s) 432
AccBSI CCGCTC 1 cut(s) 1180
AccI GTMKAC 2 cut(s) 231, 529
AciI CCGC 6 cut(s) 300, 843, 929, 1122, 1178, 1235
AclWI GGATC 1 cut(s) 500
AcoI YGGCCR 2 cut(s) 926, 1175
AcsI RAATTY 6 cut(s) 100, 488, 516, 636, 698, 1358
AfaI GTAC 4 cut(s) 282, 416, 590, 1161
AfiI CCNNNNNNNGG 9 cut(s) 46, 47, 432, 481, 774, 1036, 1063, 1096, 1100
AflIII ACRYGT 2 cut(s) 828, 1434
AgsI TTSAA 2 cut(s) 1142, 1201
AjnI CCWGG 2 cut(s) 40, 520
AjuI GAANNNNNNNTTGG 2 cut(s) 419, 451
AleI CACNNNNGTG 1 cut(s) 981
AluBI AGCT 6 cut(s) 73, 202, 877, 881, 1225, 1376
AluI AGCT 6 cut(s) 73, 202, 877, 881, 1225, 1376
Alw21I GWGCWC 3 cut(s) 75, 204, 600
AlwI GGATC 1 cut(s) 500
Ama87I CYCGRG 1 cut(s) 74
ApeKI GCWGC 4 cut(s) 23, 36, 680, 805
ApoI RAATTY 6 cut(s) 100, 488, 516, 636, 698, 1358
ArsI GACNNNNNNTTYG 2 cut(s) 1031, 1063
AspS9I GGNCC 9 cut(s) 49, 355, 571, 706, 740, 753, 849, 1027, 1087
AsuHPI GGTGA 7 cut(s) 82, 201, 641, 791, 995, 1141, 1309
AsuNHI GCTAGC 1 cut(s) 877
AvaI CYCGRG 1 cut(s) 74
AvaII GGWCC 2 cut(s) 355, 571
BanI GGYRCC 1 cut(s) 365
BanII GRGCYC 4 cut(s) 75, 204, 669, 1069
Bbv12I GWGCWC 3 cut(s) 75, 204, 600
BbvI GCAGC 4 cut(s) 10, 48, 692, 817
BccI CCATC 5 cut(s) 214, 601, 626, 1098, 1102
BceAI ACGGC 3 cut(s) 693, 727, 981
BcgI CGANNNNNNTGC 4 cut(s) 152, 186, 221, 255
BciT130I CCWGG 2 cut(s) 42, 522
BclI TGATCA 1 cut(s) 315
BfaI CTAG 7 cut(s) 467, 512, 662, 677, 797, 878, 1226
BfmI CTRYAG 1 cut(s) 34
BisI GCNGC 7 cut(s) 24, 37, 300, 681, 806, 929, 1178
BlpI GCTNAGC 1 cut(s) 1188
BlsI GCNGC 7 cut(s) 25, 38, 301, 682, 807, 930, 1179
Bme1390I CCNGG 2 cut(s) 42, 522
Bme18I GGWCC 2 cut(s) 355, 571
BmeT110I CYCGRG 1 cut(s) 74
BmgT120I GGNCC 9 cut(s) 49, 355, 571, 706, 740, 753, 849, 1027, 1087
BmiI GGNNCC 4 cut(s) 367, 437, 850, 1068
BmrFI CCNGG 2 cut(s) 42, 522
BmsI GCATC 5 cut(s) 176, 229, 261, 1155, 1393
BmtI GCTAGC 1 cut(s) 881
Bpu1102I GCTNAGC 1 cut(s) 1188
BpuEI CTTGAG 2 cut(s) 89, 793
BsaAI YACGTR 2 cut(s) 182, 829
BsaJI CCNNGG 6 cut(s) 40, 141, 387, 1014, 1035, 1256
BsaWI WCCGGW 1 cut(s) 573
Bsc4I CCNNNNNNNGG 9 cut(s) 46, 47, 432, 481, 774, 1036, 1063, 1096, 1100
Bse3DI GCAATG 4 cut(s) 128, 381, 417, 1117
BseBI CCWGG 2 cut(s) 42, 522
BseDI CCNNGG 6 cut(s) 40, 141, 387, 1014, 1035, 1256
BseGI GGATG 4 cut(s) 57, 530, 1053, 1090
BseLI CCNNNNNNNGG 9 cut(s) 46, 47, 432, 481, 774, 1036, 1063, 1096, 1100
BseMI GCAATG 4 cut(s) 128, 381, 417, 1117
BseMII CTCAG 2 cut(s) 1179, 1427
BseRI GAGGAG 3 cut(s) 149, 404, 1235
BseX3I CGGCCG 1 cut(s) 926
BseXI GCAGC 4 cut(s) 10, 48, 692, 817
BsgI GTGCAG 1 cut(s) 9
Bsh1285I CGRYCG 2 cut(s) 929, 1101
BshNI GGYRCC 1 cut(s) 365
BsiEI CGRYCG 2 cut(s) 929, 1101
BsiHKAI GWGCWC 3 cut(s) 75, 204, 600
BsiHKCI CYCGRG 1 cut(s) 74
BsiSI CCGG 2 cut(s) 574, 768
BslFI GGGAC 2 cut(s) 110, 658
BslI CCNNNNNNNGG 9 cut(s) 46, 47, 432, 481, 774, 1036, 1063, 1096, 1100
BsmFI GGGAC 2 cut(s) 110, 658
BsmI GAATGC 1 cut(s) 1207
BsoBI CYCGRG 1 cut(s) 74
Bsp1286I GDGCHC 5 cut(s) 75, 204, 600, 669, 1069
Bsp1407I TGTACA 1 cut(s) 414
Bsp143I GATC 4 cut(s) 6, 315, 505, 1167
Bsp1720I GCTNAGC 1 cut(s) 1188
Bsp19I CCATGG 1 cut(s) 1256
BspACI CCGC 6 cut(s) 300, 843, 929, 1122, 1178, 1235
BspCNI CTCAG 2 cut(s) 1180, 1426
BspLI GGNNCC 4 cut(s) 367, 437, 850, 1068
BspMAI CTGCAG 1 cut(s) 38
BspOI GCTAGC 1 cut(s) 881
BspPI GGATC 1 cut(s) 500
BspT107I GGYRCC 1 cut(s) 365
BsrBI CCGCTC 1 cut(s) 1180
BsrDI GCAATG 4 cut(s) 128, 381, 417, 1117
BsrGI TGTACA 1 cut(s) 414
BssECI CCNNGG 6 cut(s) 40, 141, 387, 1014, 1035, 1256
BssMI GATC 4 cut(s) 6, 315, 505, 1167
BssT1I CCWWGG 4 cut(s) 141, 1014, 1035, 1256
Bst2UI CCWGG 2 cut(s) 42, 522
Bst6I CTCTTC 2 cut(s) 607, 1275
BstAUI TGTACA 1 cut(s) 414
BstBAI YACGTR 2 cut(s) 182, 829
BstC8I GCNNGC 1 cut(s) 879
BstDEI CTNAG 3 cut(s) 686, 1188, 1413
BstDSI CCRYGG 1 cut(s) 1256
BstF5I GGATG 4 cut(s) 57, 530, 1053, 1090
BstKTI GATC 4 cut(s) 9, 318, 508, 1170
BstMBI GATC 4 cut(s) 6, 315, 505, 1167
BstMCI CGRYCG 2 cut(s) 929, 1101
BstMWI GCNNNNNNNGC 3 cut(s) 296, 428, 802
BstNI CCWGG 2 cut(s) 42, 522
BstNSI RCATGY 4 cut(s) 23, 604, 1430, 1438
BstSCI CCNGG 2 cut(s) 40, 520
BstSFI CTRYAG 1 cut(s) 34
BstV1I GCAGC 4 cut(s) 10, 48, 692, 817
BstX2I RGATCY 1 cut(s) 505
BstYI RGATCY 1 cut(s) 505
BstZI CGGCCG 1 cut(s) 926
BtgI CCRYGG 1 cut(s) 1256
BtsCI GGATG 4 cut(s) 57, 530, 1053, 1090
BtsI GCAGTG 1 cut(s) 105
BtsIMutI CAGTG 2 cut(s) 105, 1369
Cac8I GCNNGC 1 cut(s) 879
Cfr13I GGNCC 9 cut(s) 49, 355, 571, 706, 740, 753, 849, 1027, 1087
CseI GACGC 2 cut(s) 173, 569
Csp6I GTAC 4 cut(s) 281, 415, 589, 1160
CviAII CATG 8 cut(s) 20, 313, 343, 432, 601, 1257, 1427, 1435
CviQI GTAC 4 cut(s) 281, 415, 589, 1160
DdeI CTNAG 3 cut(s) 686, 1188, 1413
DpnI GATC 4 cut(s) 8, 317, 507, 1169
DpnII GATC 4 cut(s) 6, 315, 505, 1167
EaeI YGGCCR 2 cut(s) 926, 1175
EagI CGGCCG 1 cut(s) 926
Eam1104I CTCTTC 2 cut(s) 607, 1275
EarI CTCTTC 2 cut(s) 607, 1275
Ecl136II GAGCTC 2 cut(s) 73, 202
EclXI CGGCCG 1 cut(s) 926
Eco130I CCWWGG 4 cut(s) 141, 1014, 1035, 1256
Eco24I GRGCYC 4 cut(s) 75, 204, 669, 1069
Eco32I GATATC 2 cut(s) 553, 1081
Eco47I GGWCC 2 cut(s) 355, 571
Eco52I CGGCCG 1 cut(s) 926
Eco53kI GAGCTC 2 cut(s) 73, 202
Eco88I CYCGRG 1 cut(s) 74
EcoICRI GAGCTC 2 cut(s) 73, 202
EcoO109I RGGNCCY 1 cut(s) 849
EcoRII CCWGG 2 cut(s) 40, 520
EcoRV GATATC 2 cut(s) 553, 1081
EcoT14I CCWWGG 4 cut(s) 141, 1014, 1035, 1256
EcoT22I ATGCAT 1 cut(s) 433
EcoT38I GRGCYC 4 cut(s) 75, 204, 669, 1069
ErhI CCWWGG 4 cut(s) 141, 1014, 1035, 1256
FaeI CATG 8 cut(s) 23, 316, 346, 435, 604, 1260, 1430, 1438
FaqI GGGAC 2 cut(s) 110, 658
FatI CATG 8 cut(s) 19, 312, 342, 431, 600, 1256, 1426, 1434
FauI CCCGC 1 cut(s) 1115
FauNDI CATATG 1 cut(s) 650
FbaI TGATCA 1 cut(s) 315
FblI GTMKAC 2 cut(s) 231, 529
Fnu4HI GCNGC 7 cut(s) 24, 37, 300, 681, 806, 929, 1178
FokI GGATG 4 cut(s) 44, 537, 1040, 1077
FriOI GRGCYC 4 cut(s) 75, 204, 669, 1069
Fsp4HI GCNGC 7 cut(s) 24, 37, 300, 681, 806, 929, 1178
FspBI CTAG 7 cut(s) 467, 512, 662, 677, 797, 878, 1226
GluI GCNGC 7 cut(s) 24, 37, 300, 681, 806, 929, 1178
HapII CCGG 2 cut(s) 574, 768
HgaI GACGC 2 cut(s) 173, 569
Hin1II CATG 8 cut(s) 23, 316, 346, 435, 604, 1260, 1430, 1438
HincII GTYRAC 5 cut(s) 232, 496, 559, 902, 1215
HindII GTYRAC 5 cut(s) 232, 496, 559, 902, 1215
HindIII AAGCTT 1 cut(s) 1374
HpaII CCGG 2 cut(s) 574, 768
HphI GGTGA 7 cut(s) 82, 201, 641, 791, 995, 1141, 1309
Hpy166II GTNNAC 9 cut(s) 232, 355, 415, 496, 530, 559, 902, 1215, 1419
Hpy188I TCNGA 2 cut(s) 154, 1103
Hpy188III TCNNGA 2 cut(s) 76, 188
Hpy8I GTNNAC 9 cut(s) 232, 355, 415, 496, 530, 559, 902, 1215, 1419
Hpy99I CGWCG 6 cut(s) 125, 167, 251, 336, 596, 953
HpyAV CCTTC 2 cut(s) 184, 1316
HpyCH4IV ACGT 3 cut(s) 181, 828, 1208
HpyCH4V TGCA 7 cut(s) 26, 36, 133, 431, 1331, 1444, 1451
HpyF10VI GCNNNNNNNGC 3 cut(s) 296, 428, 802
HpyF3I CTNAG 3 cut(s) 686, 1188, 1413
HpySE526I ACGT 3 cut(s) 181, 828, 1208
Hsp92II CATG 8 cut(s) 23, 316, 346, 435, 604, 1260, 1430, 1438
Ksp22I TGATCA 1 cut(s) 315
Kzo9I GATC 4 cut(s) 6, 315, 505, 1167
LmnI GCTCC 3 cut(s) 207, 1072, 1222
Lsp1109I GCAGC 4 cut(s) 10, 48, 692, 817
LweI GCATC 5 cut(s) 176, 229, 261, 1155, 1393
MaeI CTAG 7 cut(s) 467, 512, 662, 677, 797, 878, 1226
MaeII ACGT 3 cut(s) 181, 828, 1208
MaeIII GTNAC 2 cut(s) 88, 250
MalI GATC 4 cut(s) 8, 317, 507, 1169
MbiI CCGCTC 1 cut(s) 1180
MboI GATC 4 cut(s) 6, 315, 505, 1167
MboII GAAGA 9 cut(s) 109, 515, 624, 756, 1273, 1292, 1331, 1458, 1461
MfeI CAATTG 1 cut(s) 735
MflI RGATCY 1 cut(s) 505
MhlI GDGCHC 5 cut(s) 75, 204, 600, 669, 1069
MlyI GAGTC 2 cut(s) 78, 907
MmeI TCCRAC 2 cut(s) 485, 1081
Mph1103I ATGCAT 1 cut(s) 433
MseI TTAA 2 cut(s) 746, 1356
MslI CAYNNNNRTG 3 cut(s) 605, 981, 1439
MspI CCGG 2 cut(s) 574, 768
MspR9I CCNGG 2 cut(s) 42, 522
MunI CAATTG 1 cut(s) 735
Mva1269I GAATGC 1 cut(s) 1207
MvaI CCWGG 2 cut(s) 42, 522
MwoI GCNNNNNNNGC 3 cut(s) 296, 428, 802
NcoI CCATGG 1 cut(s) 1256
NdeI CATATG 1 cut(s) 650
NdeII GATC 4 cut(s) 6, 315, 505, 1167
NheI GCTAGC 1 cut(s) 877
NlaIII CATG 8 cut(s) 23, 316, 346, 435, 604, 1260, 1430, 1438
NlaIV GGNNCC 4 cut(s) 367, 437, 850, 1068
NmeAIII GCCGAG 1 cut(s) 412
NmuCI GTSAC 2 cut(s) 88, 250
NsiI ATGCAT 1 cut(s) 433
NspI RCATGY 4 cut(s) 23, 604, 1430, 1438
OliI CACNNNNGTG 1 cut(s) 981
PciI ACATGT 1 cut(s) 1434
PctI GAATGC 1 cut(s) 1207
PfeI GAWTC 8 cut(s) 149, 371, 400, 618, 815, 973, 1351, 1463
PflFI GACNNNGTC 1 cut(s) 476
PflMI CCANNNNNTGG 1 cut(s) 432
PfoI TCCNGGA 1 cut(s) 520
PkrI GCNGC 7 cut(s) 25, 38, 301, 682, 807, 930, 1179
PleI GAGTC 2 cut(s) 78, 906
PpsI GAGTC 2 cut(s) 78, 906
Ppu21I YACGTR 2 cut(s) 182, 829
PscI ACATGT 1 cut(s) 1434
Psp124BI GAGCTC 2 cut(s) 75, 204
Psp6I CCWGG 2 cut(s) 40, 520
PspGI CCWGG 2 cut(s) 40, 520
PspN4I GGNNCC 4 cut(s) 367, 437, 850, 1068
PspPI GGNCC 9 cut(s) 49, 355, 571, 706, 740, 753, 849, 1027, 1087
PstI CTGCAG 1 cut(s) 38
PsuI RGATCY 1 cut(s) 505
PsyI GACNNNGTC 1 cut(s) 476
RsaI GTAC 4 cut(s) 282, 416, 590, 1161
RsaNI GTAC 4 cut(s) 281, 415, 589, 1160
RseI CAYNNNNRTG 3 cut(s) 605, 981, 1439
SacI GAGCTC 2 cut(s) 75, 204
SalI GTCGAC 1 cut(s) 230
SaqAI TTAA 2 cut(s) 746, 1356
SatI GCNGC 7 cut(s) 24, 37, 300, 681, 806, 929, 1178
Sau3AI GATC 4 cut(s) 6, 315, 505, 1167
Sau96I GGNCC 9 cut(s) 49, 355, 571, 706, 740, 753, 849, 1027, 1087
SchI GAGTC 2 cut(s) 78, 907
ScrFI CCNGG 2 cut(s) 42, 522
SduI GDGCHC 5 cut(s) 75, 204, 600, 669, 1069
SfaNI GCATC 5 cut(s) 176, 229, 261, 1155, 1393
SfcI CTRYAG 1 cut(s) 34
SinI GGWCC 2 cut(s) 355, 571
SmiMI CAYNNNNRTG 3 cut(s) 605, 981, 1439
SmlI CTYRAG 2 cut(s) 68, 772
SmoI CTYRAG 2 cut(s) 68, 772
SsiI CCGC 6 cut(s) 300, 843, 929, 1122, 1178, 1235
SspI AATATT 1 cut(s) 957
SspMI CTAG 7 cut(s) 467, 512, 662, 677, 797, 878, 1226
SstI GAGCTC 2 cut(s) 75, 204
StyD4I CCNGG 2 cut(s) 40, 520
StyI CCWWGG 4 cut(s) 141, 1014, 1035, 1256
TaiI ACGT 3 cut(s) 184, 831, 1211
TaqI TCGA 6 cut(s) 147, 162, 198, 231, 501, 873
TaqII GACCGA 2 cut(s) 1087, 1230
TatI WGTACW 1 cut(s) 414
TauI GCSGC 3 cut(s) 302, 931, 1180
TfiI GAWTC 8 cut(s) 149, 371, 400, 618, 815, 973, 1351, 1463
Tru1I TTAA 2 cut(s) 746, 1356
Tru9I TTAA 2 cut(s) 746, 1356
TscAI CASTG 2 cut(s) 112, 1369
TseFI GTSAC 2 cut(s) 88, 250
TseI GCWGC 4 cut(s) 23, 36, 680, 805
Tsp45I GTSAC 2 cut(s) 88, 250
TspDTI ATGAA 2 cut(s) 828, 876
TspGWI ACGGA 1 cut(s) 1470
TspRI CASTG 2 cut(s) 112, 1369
Tth111I GACNNNGTC 1 cut(s) 476
Van91I CCANNNNNTGG 1 cut(s) 432
VpaK11BI GGWCC 2 cut(s) 355, 571
XapI RAATTY 6 cut(s) 100, 488, 516, 636, 698, 1358
XceI RCATGY 4 cut(s) 23, 604, 1430, 1438
XcmI CCANNNNNNNNNTGG 3 cut(s) 349, 1021, 1170
XmiI GTMKAC 2 cut(s) 231, 529
XspI CTAG 7 cut(s) 467, 512, 662, 677, 797, 878, 1226
Zsp2I ATGCAT 1 cut(s) 433
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.