Rw2G015970

NEMP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001753
Physical Location & Seq
Forward (+)
18673967 .. 18678374
4408 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G015970.1

Sequence Viewer

Length: 936 bp
ATGGCTTCTTCTAAGCCGGTTCGGGTCGCTGCGTGCATTCTATGGCTTCTGTTCCTCACGCTTCCCTCTTCTTCTTCTTCCTCTGAATCGCCGACTGAGTATTCTCTAGTTATTGGTGAGAATGCTACTCTGCAATTATCTAATGGTGTGCTGGTGCAAAATTCTCCTGGTGCTAAGCCTGGAACTTCACTGTTGTGTGGAAGAGTTCATGTATGTGGATTGTCAAGGCTTAAATATCTGGAGAAGTTTGCCGACACGGTGAAGGTGAAGATATCAACAAATTCGAGCTTTCGGATACGGCCTTTTGAGGTTTGCTTCCACAGAAATACTTCTCGTGGGATAGGAATGTGCCCTCACAGCCAGTGGGAGAAAGTTTCCAAGGGGTCCTGGTCTGGATCCATGTCACCTTATGAGCGCAAACTCTTAGATATACGGACAGTGGGTTCATCCTTAGAGAGTTTTGAGGTGTCCCTTGAAGAAGAATTTTTTATATCTCGCCTAGTATTTTTTGTGTCTGGTGTAATCTTGATGAGTTTGGCACCTTCTCTGAGCAATTCATTGGTATTCTATTACGGAAGTGGAATGGCAATAGGGGTGGTCCTTGTACTATTGATCGTCCTTTTTCAGGGGATGAAGCTTCTCCCAACAGGTCGGAAGAGTTCGCTCGGATTTTTTTTGTTTTCATCTGTGGTTGGTTTGGGATCTTTTCTCCTCAGCTACATCCCAGGGCTATTGCGTTCAGTACTAACAGAGCTTGGAATTAGCGAAGAGATGTATAATCCTAGTTTGCTGCATGGTGGCTTTCAAGGAATATATTTGTGCTTGAGTAATCACTCGCTTGGGATACTAACTAAAAGTTTTGGAGCCTTAGCAGTTAATGTTTTGATTATACTTTGGTTGCAGGGAAGAAAATGGTGGAGTACATGGTGCCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NEMP

Protein Analysis

311

Amino Acids

34.08

Weight (kDa)

9.28

Isoelectric Point (pI)

44.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NEMP PF10225 155 - 252 8.4e-24 NEMP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 538, 927
AclWI GGATC 3 cut(s) 390, 403, 709
AcsI RAATTY 3 cut(s) 160, 280, 482
AfaI GTAC 3 cut(s) 606, 744, 922
AfiI CCNNNNNNNGG 1 cut(s) 625
AgsI TTSAA 2 cut(s) 476, 806
AjnI CCWGG 4 cut(s) 166, 178, 386, 724
AleI CACNNNNGTG 1 cut(s) 193
AloI GAACNNNNNNTCC 2 cut(s) 427, 459
AluBI AGCT 4 cut(s) 288, 637, 717, 754
AluI AGCT 4 cut(s) 288, 637, 717, 754
AlwI GGATC 3 cut(s) 390, 403, 709
AoxI GGCC 1 cut(s) 299
ApeKI GCWGC 2 cut(s) 29, 790
ApoI RAATTY 3 cut(s) 160, 280, 482
Asp700I GAANNNNTTC 1 cut(s) 328
AspLEI GCGC 1 cut(s) 417
AspS9I GGNCC 2 cut(s) 384, 598
AsuHPI GGTGA 4 cut(s) 128, 271, 277, 396
AvaII GGWCC 2 cut(s) 384, 598
BaeGI GKGCMC 1 cut(s) 353
BamHI GGATCC 1 cut(s) 395
BanI GGYRCC 2 cut(s) 538, 927
BauI CACGAG 1 cut(s) 333
BbvCI CCTCAGC 1 cut(s) 713
BbvI GCAGC 2 cut(s) 16, 777
BceAI ACGGC 1 cut(s) 314
BciT130I CCWGG 4 cut(s) 168, 180, 388, 726
BciVI GTATCC 2 cut(s) 288, 837
BfaI CTAG 3 cut(s) 107, 500, 783
BfuI GTATCC 2 cut(s) 288, 837
BisI GCNGC 2 cut(s) 30, 791
BlpI GCTNAGC 1 cut(s) 174
BlsI GCNGC 2 cut(s) 31, 792
BmcAI AGTACT 1 cut(s) 744
Bme1390I CCNGG 4 cut(s) 168, 180, 388, 726
Bme18I GGWCC 2 cut(s) 384, 598
BmgT120I GGNCC 2 cut(s) 384, 598
BmiI GGNNCC 5 cut(s) 385, 397, 540, 865, 929
BmrFI CCNGG 4 cut(s) 168, 180, 388, 726
BpmI CTGGAG 1 cut(s) 260
Bpu10I CCTNAGC 2 cut(s) 713, 868
Bpu1102I GCTNAGC 1 cut(s) 174
BpuEI CTTGAG 1 cut(s) 844
BsaJI CCNNGG 3 cut(s) 378, 724, 725
Bsc4I CCNNNNNNNGG 1 cut(s) 625
Bse118I RCCGGY 1 cut(s) 16
Bse1I ACTGG 1 cut(s) 361
BseBI CCWGG 4 cut(s) 168, 180, 388, 726
BseDI CCNNGG 3 cut(s) 378, 724, 725
BseGI GGATG 3 cut(s) 446, 636, 720
BseLI CCNNNNNNNGG 1 cut(s) 625
BseMII CTCAG 3 cut(s) 87, 539, 727
BseNI ACTGG 1 cut(s) 361
BseRI GAGGAG 1 cut(s) 701
BseSI GKGCMC 1 cut(s) 353
BseXI GCAGC 2 cut(s) 16, 777
BshFI GGCC 1 cut(s) 301
BshNI GGYRCC 2 cut(s) 538, 927
BsiSI CCGG 1 cut(s) 17
BslFI GGGAC 1 cut(s) 454
BslI CCNNNNNNNGG 1 cut(s) 625
BsmFI GGGAC 1 cut(s) 454
BsmI GAATGC 2 cut(s) 36, 127
BsnI GGCC 1 cut(s) 301
Bsp1286I GDGCHC 1 cut(s) 353
Bsp143I GATC 3 cut(s) 395, 612, 701
Bsp1720I GCTNAGC 1 cut(s) 174
BspANI GGCC 1 cut(s) 301
BspCNI CTCAG 3 cut(s) 88, 540, 726
BspLI GGNNCC 5 cut(s) 385, 397, 540, 865, 929
BspPI GGATC 3 cut(s) 390, 403, 709
BspT107I GGYRCC 2 cut(s) 538, 927
BsrFI RCCGGY 1 cut(s) 16
BsrI ACTGG 1 cut(s) 361
BssAI RCCGGY 1 cut(s) 16
BssECI CCNNGG 3 cut(s) 378, 724, 725
BssMI GATC 3 cut(s) 395, 612, 701
BssSI CACGAG 1 cut(s) 333
BssT1I CCWWGG 1 cut(s) 378
Bst2BI CACGAG 1 cut(s) 333
Bst2UI CCWGG 4 cut(s) 168, 180, 388, 726
Bst4CI ACNGT 3 cut(s) 192, 259, 439
Bst6I CTCTTC 4 cut(s) 73, 196, 650, 762
BstC8I GCNNGC 1 cut(s) 34
BstDEI CTNAG 8 cut(s) 12, 96, 174, 424, 451, 548, 713, 868
BstENI CCTNNNNNAGG 1 cut(s) 623
BstF5I GGATG 3 cut(s) 446, 636, 720
BstHHI GCGC 1 cut(s) 417
BstKTI GATC 3 cut(s) 398, 615, 704
BstMBI GATC 3 cut(s) 395, 612, 701
BstMWI GCNNNNNNNGC 1 cut(s) 357
BstNI CCWGG 4 cut(s) 168, 180, 388, 726
BstSCI CCNGG 4 cut(s) 166, 178, 386, 724
BstSLI GKGCMC 1 cut(s) 353
BstV1I GCAGC 2 cut(s) 16, 777
BstX2I RGATCY 2 cut(s) 395, 701
BstYI RGATCY 2 cut(s) 395, 701
BsuI GTATCC 2 cut(s) 288, 837
BsuRI GGCC 1 cut(s) 301
BtsCI GGATG 3 cut(s) 446, 636, 720
BtsIMutI CAGTG 3 cut(s) 188, 368, 444
Cac8I GCNNGC 1 cut(s) 34
CfoI GCGC 1 cut(s) 417
Cfr10I RCCGGY 1 cut(s) 16
Cfr13I GGNCC 2 cut(s) 384, 598
Csp6I GTAC 3 cut(s) 605, 743, 921
CspCI CAANNNNNGTGG 2 cut(s) 576, 611
CviAII CATG 4 cut(s) 209, 400, 794, 924
CviQI GTAC 3 cut(s) 605, 743, 921
DdeI CTNAG 8 cut(s) 12, 96, 174, 424, 451, 548, 713, 868
DpnI GATC 3 cut(s) 397, 614, 703
DpnII GATC 3 cut(s) 395, 612, 701
Eam1104I CTCTTC 4 cut(s) 73, 196, 650, 762
EarI CTCTTC 4 cut(s) 73, 196, 650, 762
Eco130I CCWWGG 1 cut(s) 378
Eco32I GATATC 1 cut(s) 273
Eco47I GGWCC 2 cut(s) 384, 598
EcoNI CCTNNNNNAGG 1 cut(s) 623
EcoO109I RGGNCCY 1 cut(s) 384
EcoRII CCWGG 4 cut(s) 166, 178, 386, 724
EcoRV GATATC 1 cut(s) 273
EcoT14I CCWWGG 1 cut(s) 378
ErhI CCWWGG 1 cut(s) 378
FaeI CATG 4 cut(s) 212, 403, 797, 927
FaqI GGGAC 1 cut(s) 454
FatI CATG 4 cut(s) 208, 399, 793, 923
Fnu4HI GCNGC 2 cut(s) 30, 791
FokI GGATG 3 cut(s) 433, 643, 707
Fsp4HI GCNGC 2 cut(s) 30, 791
FspBI CTAG 3 cut(s) 107, 500, 783
GlaI GCGC 1 cut(s) 416
GluI GCNGC 2 cut(s) 30, 791
GsuI CTGGAG 1 cut(s) 260
HaeIII GGCC 1 cut(s) 301
HapII CCGG 1 cut(s) 17
HhaI GCGC 1 cut(s) 417
Hin1II CATG 4 cut(s) 212, 403, 797, 927
Hin6I GCGC 1 cut(s) 415
HinP1I GCGC 1 cut(s) 415
HindIII AAGCTT 1 cut(s) 635
HinfI GANTC 1 cut(s) 86
HpaII CCGG 1 cut(s) 17
HphI GGTGA 4 cut(s) 128, 271, 277, 396
Hpy188I TCNGA 5 cut(s) 85, 294, 549, 654, 668
Hpy188III TCNNGA 3 cut(s) 239, 393, 526
HpyAV CCTTC 2 cut(s) 256, 552
HpyCH4III ACNGT 3 cut(s) 192, 259, 439
HpyCH4V TGCA 5 cut(s) 36, 133, 157, 793, 901
HpyF10VI GCNNNNNNNGC 1 cut(s) 357
HpyF3I CTNAG 8 cut(s) 12, 96, 174, 424, 451, 548, 713, 868
Hsp92II CATG 4 cut(s) 212, 403, 797, 927
HspAI GCGC 1 cut(s) 415
Kzo9I GATC 3 cut(s) 395, 612, 701
LmnI GCTCC 1 cut(s) 863
Lsp1109I GCAGC 2 cut(s) 16, 777
MaeI CTAG 3 cut(s) 107, 500, 783
MaeIII GTNAC 1 cut(s) 402
MalI GATC 3 cut(s) 397, 614, 703
MboI GATC 3 cut(s) 395, 612, 701
MflI RGATCY 2 cut(s) 395, 701
MhlI GDGCHC 1 cut(s) 353
MluCI AATT 6 cut(s) 134, 160, 280, 482, 553, 759
MmeI TCCRAC 1 cut(s) 632
MnlI CCTC 7 cut(s) 65, 76, 91, 301, 363, 457, 722
MroXI GAANNNNTTC 1 cut(s) 328
MseI TTAA 2 cut(s) 231, 876
MslI CAYNNNNRTG 2 cut(s) 193, 213
MspI CCGG 1 cut(s) 17
MspR9I CCNGG 4 cut(s) 168, 180, 388, 726
Mva1269I GAATGC 2 cut(s) 36, 127
MvaI CCWGG 4 cut(s) 168, 180, 388, 726
MwoI GCNNNNNNNGC 1 cut(s) 357
NdeII GATC 3 cut(s) 395, 612, 701
NlaIII CATG 4 cut(s) 212, 403, 797, 927
NlaIV GGNNCC 5 cut(s) 385, 397, 540, 865, 929
NmuCI GTSAC 1 cut(s) 402
OliI CACNNNNGTG 1 cut(s) 193
PasI CCCWGGG 1 cut(s) 725
PctI GAATGC 2 cut(s) 36, 127
PdmI GAANNNNTTC 1 cut(s) 328
PfeI GAWTC 1 cut(s) 86
PkrI GCNGC 2 cut(s) 31, 792
PpuMI RGGWCCY 1 cut(s) 384
Psp5II RGGWCCY 1 cut(s) 384
Psp6I CCWGG 4 cut(s) 166, 178, 386, 724
PspGI CCWGG 4 cut(s) 166, 178, 386, 724
PspN4I GGNNCC 5 cut(s) 385, 397, 540, 865, 929
PspPI GGNCC 2 cut(s) 384, 598
PspPPI RGGWCCY 1 cut(s) 384
PsuI RGATCY 2 cut(s) 395, 701
RsaI GTAC 3 cut(s) 606, 744, 922
RsaNI GTAC 3 cut(s) 605, 743, 921
RseI CAYNNNNRTG 2 cut(s) 193, 213
SaqAI TTAA 2 cut(s) 231, 876
SatI GCNGC 2 cut(s) 30, 791
Sau3AI GATC 3 cut(s) 395, 612, 701
Sau96I GGNCC 2 cut(s) 384, 598
ScaI AGTACT 1 cut(s) 744
ScrFI CCNGG 4 cut(s) 168, 180, 388, 726
SduI GDGCHC 1 cut(s) 353
SinI GGWCC 2 cut(s) 384, 598
SmiMI CAYNNNNRTG 2 cut(s) 193, 213
SmlI CTYRAG 1 cut(s) 823
SmoI CTYRAG 1 cut(s) 823
Sse9I AATT 6 cut(s) 134, 160, 280, 482, 553, 759
SspMI CTAG 3 cut(s) 107, 500, 783
StyD4I CCNGG 4 cut(s) 166, 178, 386, 724
StyI CCWWGG 1 cut(s) 378
TaaI ACNGT 3 cut(s) 192, 259, 439
TaqI TCGA 1 cut(s) 284
TasI AATT 6 cut(s) 134, 160, 280, 482, 553, 759
TatI WGTACW 3 cut(s) 604, 742, 920
TfiI GAWTC 1 cut(s) 86
Tru1I TTAA 2 cut(s) 231, 876
Tru9I TTAA 2 cut(s) 231, 876
TscAI CASTG 3 cut(s) 195, 368, 444
TseFI GTSAC 1 cut(s) 402
TseI GCWGC 2 cut(s) 29, 790
Tsp45I GTSAC 1 cut(s) 402
TspDTI ATGAA 5 cut(s) 197, 435, 546, 647, 672
TspGWI ACGGA 2 cut(s) 448, 588
TspRI CASTG 3 cut(s) 195, 368, 444
VpaK11BI GGWCC 2 cut(s) 384, 598
XagI CCTNNNNNAGG 1 cut(s) 623
XapI RAATTY 3 cut(s) 160, 280, 482
XmnI GAANNNNTTC 1 cut(s) 328
XspI CTAG 3 cut(s) 107, 500, 783
ZrmI AGTACT 1 cut(s) 744
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.